LOCUS       MH325893                8034 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter werkmanii strain O14_G3537 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325893
VERSION     MH325893.1
KEYWORDS    .
SOURCE      Citrobacter werkmanii
  ORGANISM  Citrobacter werkmanii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Citrobacter werkmanii"
                     /mol_type="genomic DNA"
                     /strain="O14_G3537"
                     /db_xref="taxon:67827"
                     /country="Poland"
     misc_feature    1..8034
                     /note="antigen biosynthesis gene cluster"
     gene            1186..1584
                     /gene="orf1"
     CDS             1186..1584
                     /gene="orf1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="AWU66640.1"
                     /translation="MMKKIITFGTFDVFHIGHVNILERAATYGDYLIVGVSSDKLNFK
                     KKGRNPIYNQSERCKIINSLKFVNEVFIEESLELKKEYIVKYNADVLIMGDDWEGRFD
                     WVKDVCDVVYLPRTPSISTTEIIEVVRSIR"
     gene            1686..2777
                     /gene="orf2"
     CDS             1686..2777
                     /gene="orf2"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="AWU66641.1"
                     /translation="MNIKNVSYAFISMILLPICLILRFFSKNNRCFFYSPLGLKGNIK
                     YLHEFYKRKGEECYFIDPIKLNLKGFLKLNIELSRAKVIFLTHGIGKLPLICFLIPRI
                     QLWHGFPLKKILLNSDVDTKKFKSELLNYIFKVIYRFRIYISYTYLVTSDSLLGHELI
                     DSFGFNKQKVLLLGSPSQERAEIVKCKKNNSSFRILYLPTWRDGSDRIFDILSEFISE
                     IDDCFCKENRIEMDIKLHPYDMQKNKYKALNNSECITIINNDIEDMIDFYSNYQCLIT
                     DYSSACFEFAPVSNKVIFYVPDFQQYINDRGFTVDINLLFNGREVTNIEQLKMALLEV
                     NNNDRYTFDYVNYVGKSNECNELIYEKFH"
     gene            2761..3543
                     /gene="gt1"
     CDS             2761..3543
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66642.1"
                     /translation="MKNFTNPMISIIMPAYNGGEYITQAIQSVIDQTYKNWELIIIDD
                     HSVDDSYSIAQHFTVNHPNIKLYQTEHKGSGAAVARNIGIAAATGRFIAFLDCDDFWL
                     PKKLSKQINLLLSTDAHFCYSAYAIFNAKDNKKTGEFKPSAKISYDKLLRGCDIGCLT
                     VIYDTEFLGKKFFPMVEKEDYALWLDIIKTEGVKFTVCNEITAYYRLSEQSISSNKWK
                     ELSRQLRIYRNVEQMSIFKSFFYLIRYALYGIQKHYFSYRRG"
     gene            3503..4852
                     /gene="orf4"
     CDS             3503..4852
                     /gene="orf4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AWU66643.1"
                     /translation="MVYKSIILAIEGGNVERVSFRSNKSFVFCIVLFFSIFCSFNFFP
                     FITAEVQPVIGVFILLYGVVYFSTTLKINYPSLFILIFCLALLPSLVLGISVKAVNGV
                     SYLFYLIGPAIFCYFYKYFYLLTRSHLKFFIFIFFIISLLQALSPETINSIINPYFEM
                     LIKRARFGFYGDMRGVGILYSEPAHAAKYIFVLTVLLISFKLTPFFFSEPNSGETKSS
                     FWLALMLFICILLNKSASLYGTYVLLMFFYLFFYVIKPTPIRLIIILSSIPVLAGLLY
                     IGALLELYTYLPERLASLVSSYNSVKDNFTLNDLQYFGSIRLISVIAGYAGPFIQPLG
                     VGIGNGGTEIFNIMQVVGFDLNSINFLTKQDVEFLKPNAYGAQIAIEAGVLGLLAIIV
                     FLIYFLFCIRVKTKIECFYSSLLALSLFQILFFSTTTVTAPWMTLALSLYSLKRLKS"
     gene            4849..5592
                     /gene="wzm"
     CDS             4849..5592
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="AWU66644.1"
                     /translation="MKFLMANWYIFSIPFLYGMMLLIEFISQYSRVAGYFLDRNAIAY
                     SLQNTTFTLTRFFSVLLMPLIGLMVDSNYTPGIFIACVLISYFFVSILGIMVFINRMH
                     ICAFYIRFIRSYINGGGIIKSFINTIRNKHEELGCDIQEVKSYKIDFKYFYISSFVYS
                     IHAIGVFMTFYLALISPTNKIMITQMSGVINAFATLLLTFKIDPALSVAIERRYNFIS
                     AFMSIFYARIFVFLFIAPFIFIMLYITTA"
     gene            6628..8034
                     /gene="gt2"
     CDS             6628..8034
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66645.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNTESRGYTVSIFNRSREKTEEVVA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELATT
                     FTEWNEGELSSYLIDITKDIFTKKDEDGKYLIDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAKLAGDKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENAGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
BASE COUNT     2359 a   1369 c   1613 g   2693 t
ORIGIN
        1 atgactaatt tgaaagcagt tattccggta gcgggcctgg ggatgcacat gttgcctgcc
       61 acgaaggcaa tcccgaaaga gatgctgcca atcgtagaca agccgatgat tcaatacatt
      121 gtcgacgaga ttgtagctgc tggcattaaa gaaatcgttc tggtaacgca tgcttccaag
      181 aacgcagttg agaaccattt cgacacctct tacgaactcg aatcgttgct tgagcagcgt
      241 gttaagcgcc agttattagc tgaggtgcag tctatctgtc ctcctggcgt aactatcatg
      301 aacgttcgtc aggcgcaatt actcggcctg ggtcattcaa tcctttgtgc tcgcccgatt
      361 gttggggaca acccgttcgt cgtggtgctc ccggatgtcg ttcttgatac cgcaagtgcg
      421 gaccctctgc gctacaacct tgcagccatg gtagctcgtt ttaatgaaac cgggcgtagt
      481 caagtgctgg caaaacgtat ggaaggcgat ctgtctgagt actcggttat tcagaccaaa
      541 gagcctttag acaacgaagg taaagttagc cgtattgttg aattcatcga gaaacccgat
      601 caaccacaaa cgctggattc cgatctgatg gccgtagggc gttatgtgct gtctgctgat
      661 atttgggccg aactcgagca tacacaacct ggtgcctggg gccgtatcca gttaacggat
      721 gctattgcag aactggcgaa aaaacagtct gtcgatgcta tgctgatgac gggagacagt
      781 tacgattgtg gtaaaaaaat gggctatatg caggcttttg tgaagtacgg gctgcgtaat
      841 ctgaaagagg gtgccaagtt ccgtaagagc attgagaatt tgcttagcga atagattaac
      901 acacttgaca tacatagcat tttaataaaa tggacggtga tctatatcga aaacgtagag
      961 cattatagtc gcgatatggt tgccgtttgt tataaatttt cagaaaaaaa cgctaaccca
     1021 ataaaaacct cggaattttc atgtttttct tgtttctctt ctcatttggt aagagaatta
     1081 gtaatacaat tcgaggcttg tgatgggggc agtaaaattc ttttgtatta agtgcactgg
     1141 tagctgttga gccaggggcg gtagctttca taaccacgag atttaatgat gaaaaagatt
     1201 ataacttttg gcacatttga tgttttccat attggccatg ttaatatttt agagcgggca
     1261 gctacttatg gtgattactt aatagtaggt gtgagctctg ataagcttaa ctttaagaaa
     1321 aaaggacgta atccaattta taatcaaagt gagcgatgca aaattataaa ctctcttaaa
     1381 tttgttaatg aggtttttat tgaagaatca ttggaactaa aaaaagagta tattgtaaaa
     1441 tataatgcag atgttctaat tatgggcgat gattgggaag ggcgatttga ctgggtaaaa
     1501 gatgtatgtg atgttgttta tcttccaaga accccatcaa tatctacaac tgaaataatt
     1561 gaagttgtac gttctatcag ataataactt gttgagatat atatggttta tatattcttt
     1621 aacaactatt aagtacttta atctatatgt tataatgcaa aattctttta tttaccagtg
     1681 tttttatgaa tattaaaaat gtttcttatg cttttatttc aatgatctta ttgccgattt
     1741 gtttaatact ccgttttttt tctaaaaata atcgttgttt tttttattcc cctctgggat
     1801 tgaaagggaa cataaaatat cttcatgaat tttataaaag gaaaggtgaa gaatgttatt
     1861 ttatagatcc gataaaatta aacctcaagg ggtttttaaa gcttaacatt gaactttcac
     1921 gagctaaagt aatattttta acccatggta taggaaaact accattaatt tgttttttaa
     1981 ttcctcgaat tcagttatgg catgggtttc ctctaaaaaa aatactttta aacagtgatg
     2041 tagatactaa gaaattcaaa agcgagctgc ttaattatat ttttaaagtg atttatcgat
     2101 tccgaatata catcagttat acttacttgg tcacgtctga ttcgttgctc ggccatgaac
     2161 tgatagatag ctttggtttt aataaacaaa aggttttact tttaggtagt cctagccagg
     2221 aacgtgctga gattgtaaag tgtaaaaaaa ataactccag cttcaggatt ctttatttac
     2281 caacatggag ggatggaagt gatagaattt ttgatatctt atctgaattt atttctgaga
     2341 ttgatgactg tttttgtaag gaaaatagaa tagaaatgga tatcaaacta catccttatg
     2401 atatgcaaaa aaataaatac aaggctctta ataatagtga atgtattact attattaata
     2461 acgatattga agatatgata gatttttact ccaattatca atgtcttatt accgactatt
     2521 ctagcgcatg ttttgaattt gctcccgtca gtaataaggt tatattttac gttcctgatt
     2581 ttcagcaata cataaatgat agaggtttta ctgttgatat aaatttatta tttaatggta
     2641 gggaggttac aaatattgaa cagcttaaaa tggcattgct agaagtaaat aataatgata
     2701 gatatacatt cgattatgtt aattacgttg gcaaaagtaa tgaatgcaac gagttgattt
     2761 atgaaaaatt tcactaaccc catgattagt attataatgc ctgcatataa tgggggggag
     2821 tatataaccc aggctataca atcagtaatt gatcaaacct ataaaaattg ggaacttatc
     2881 attatagatg accattctgt agatgatagc tattctattg cccagcattt cacggtcaat
     2941 cacccaaata tcaaacttta tcagacagaa cataaaggta gtggggctgc tgtagcgagg
     3001 aatataggta tagctgcggc aactggacgg tttattgctt ttttggattg tgatgatttc
     3061 tggttgccta aaaaattaag caagcaaata aatttgttac tctctactga tgctcatttc
     3121 tgctacagtg cttatgctat ctttaatgct aaagataata aaaaaacagg agaatttaaa
     3181 ccatcagcaa aaatcagcta tgataaattg cttagaggtt gtgatatagg ttgtttaact
     3241 gttatatatg atactgaatt tctagggaaa aaatttttcc cgatggttga aaaagaagat
     3301 tatgctttat ggttagacat aataaaaacg gaaggtgtga agttcactgt ttgcaatgaa
     3361 attactgcgt attatcgtct ttcagaacag tcaatttcat ctaacaaatg gaaggagtta
     3421 agtagacaat tacgaattta cagaaatgtt gaacaaatga gtatatttaa atcttttttt
     3481 tatcttattc gatacgccct ttatggtata caaaagcatt attttagcta tagaaggggg
     3541 taatgtggaa cgtgtaagct tcaggtctaa taaaagtttt gttttttgta ttgttttatt
     3601 tttttcaatt ttttgctcat ttaacttttt cccttttata acagcagaag tccagcctgt
     3661 aattggtgta tttattttac tatatggggt ggtgtatttt tctaccacgc tgaaaattaa
     3721 ttatccaagt ttatttattc ttattttttg cctggcgtta ttaccatctt tagttttggg
     3781 tatttcggtt aaggccgtaa atggggtttc ttatttattc tatttaattg gtccagccat
     3841 attttgttat ttctataagt atttttatct actgactaga tcgcatttga aatttttcat
     3901 ttttattttt tttataatat ctttattgca agctctctca ccggaaacaa taaatagtat
     3961 tatcaaccca tactttgaaa tgctaattaa acgagctcga tttggttttt atggagacat
     4021 gagaggtgtt gggatacttt actctgaacc agctcatgct gctaaatata tatttgtact
     4081 tacggtatta ttaatttctt ttaaattgac accgtttttc tttagcgaac caaattcggg
     4141 agaaacaaaa tcttcttttt ggttagctct catgttattt atctgcattc tgttaaataa
     4201 gagtgcttca ttatatggaa cttatgtttt attaatgttt ttttatttgt ttttttatgt
     4261 tataaagccc acgccgatac gattgattat aattctaagt agcatcccag tattggctgg
     4321 cctattatat attggtgcat tgcttgaatt atatacttac cttccagaac gcttagctag
     4381 tttagtatca tcctataatt cagtgaaaga caatttcact ttaaatgatc tacaatattt
     4441 tggcagtatt agacttatct ctgtaatagc aggttatgcg ggaccattta tacaaccctt
     4501 aggagttggc attggtaatg gaggaactga aatattcaat ataatgcaag tggttggttt
     4561 tgacctcaat agcatcaact ttctgactaa acaagatgtg gaattcctga aacctaatgc
     4621 ctatggggct caaattgcta tcgaggcagg agttttgggg ttgttggcga ttattgtatt
     4681 tttaatatat tttcttttct gtataagagt taagactaaa attgaatgtt tctatagctc
     4741 tttactagca ctttctttat tccaaatatt attttttagt acaactacag tgactgctcc
     4801 ttggatgaca ttggcattaa gtttatattc tttaaaaagg ttaaaatcat gaaatttctt
     4861 atggcaaatt ggtatatttt ttcgattcca tttctatacg gaatgatgct tttaattgaa
     4921 tttatttcac agtatagcag agttgctggt tattttttag ataggaatgc tattgcatat
     4981 tctttgcaaa atacaacttt tacattgact cgtttttttt ctgtattgtt aatgcctttg
     5041 attggtctta tggttgacag taattacacg ccagggatat ttattgcatg tgtattaata
     5101 tcatacttct ttgtttctat tctaggtatc atggtattta ttaatcgaat gcatatttgt
     5161 gccttttata taagatttat tcgctcttat ataaacggcg gtgggataat aaaatcattt
     5221 attaacacca ttcgaaataa gcatgaagag ttaggttgtg atatacaaga agtaaagagt
     5281 tataagattg attttaaata tttttacata tcttcctttg tttattctat ccatgccata
     5341 ggcgttttta tgacgtttta tcttgcactt atatcaccaa ctaataagat aatgatcacc
     5401 caaatgtcag gtgtaataaa tgcgtttgcg acattgctat taacttttaa gatcgaccca
     5461 gccttatctg ttgcgattga aagaaggtac aattttatct cggcatttat gtccattttt
     5521 tacgcaagaa tttttgtttt cctctttatc gcacctttta tatttataat gttatatatt
     5581 actacggcgt agcttatgag attctcaata attatacctc tttataataa aggtcaatat
     5641 atttcaaaaa cattggagtc cctattcaag cagacattcc aagattttga agtaatagtt
     5701 gttaatgatg gttctacaga caatggacct tcattggtta atcaggttgc tgatgaacgt
     5761 gtcaaattaa tatgccaagc aaaccaaggt gtttcagttg ctaggaatac tggagcgaaa
     5821 catgctgttg gtgatttttt ggtgtttctt gatgccgatg atgtttatct ggataatgct
     5881 ttagcgcatt tctccttttt aattgatcgc ttccccgaac atgactactt ttgtgcaaac
     5941 tattatcgtg tttcaaatgg ggttagaaaa acagccatcg atgtagacaa gattctagga
     6001 aaagacttta aatatggtat cgtagacaat tttttctata tcgcatcatt tcatccggga
     6061 tcctttccat gtaatgtttc gacttttact attagaagag aaaggttgat tgagagaaat
     6121 atttatttcc ctgaaggtgt ttcacatact gaagatgtct atttttgctc tgttttggct
     6181 ttaaatttct ttcctgtatt ttcaagggaa tgtgttcatg agtattatat aaataatata
     6241 ggtaattcac gatcttctcg acctactgat actcgttttg ttattgatga attaagaaag
     6301 aaaactgatg aggacaagta tattagctca ttcattgcaa agaacattat ccacttatta
     6361 tataattgta ttgagtttga cgatttttct aattataaga agcataagag tgatagtttt
     6421 tttttgttgc aatatatgtt gggaaaatat aagtatcatt atcttattct taaatttctt
     6481 ccctatgcag tagcaaagaa attatattct ttggctaaga aaaaaagata aaaattattg
     6541 ttcatcaatt taattcgaat aagctatcat aaggtattta aagccgcgca tacatcgcgt
     6601 ggtgaccaca cctgacagga gtatgtaatg tccaagcaac agatcggcgt cgtcggaatg
     6661 gcagtgatgg ggcgcaacct ggcgctcaat accgaaagcc gtggttatac cgtctccatc
     6721 ttcaaccgtt cccgtgaaaa aaccgaagaa gttgtagccg agaatccagg taagaaactg
     6781 gttccttatt acacggtgaa agagttcgtc gagtctcttg aaaccccgcg tcgtatcctg
     6841 ttaatggtga aagcgggcgc tggcacagat gctgccatcg attccctgaa gccgtacctt
     6901 gataaaggcg acatcatcat tgatggcggc aacaccttct tccaggacac cattcgtcgt
     6961 aatcgcgaac tgtctgcaga aggctttaac ttcatcggta ccggtgtttc cggcggtgaa
     7021 gagggggcgc tgaaaggccc gtctatcatg cctggtggcc agaaggaagc ctatgaactg
     7081 gttgctccga tcctgaccaa aatcgctgct gtggcggaag acggtgaacc gtgcgtgacc
     7141 tatatcggcg ctgacggtgc gggccattac gtgaagatgg tccacaacgg tatcgaatac
     7201 ggcgacatgc agctgattgc tgaagcctat tctctgctga aaggcggtct gaacctgtcc
     7261 aatgaagagc tggccaccac cttcaccgag tggaacgaag gcgagttgag cagctacctg
     7321 atcgacatca ccaaagacat cttcaccaaa aaagatgaag atggtaaata cctgattgac
     7381 gtgattctgg acgaagcggc gaacaaaggc accggtaaat ggaccagcca gagctctctg
     7441 gatctgggcg aaccgctgtc gctgatcact gagtctgtct tcgcgcgtta tatctcttct
     7501 ctgaaagatc agcgcgttgc tgcatctaaa gtgctgtccg gcccgcaggc caaactggct
     7561 ggcgataaag ctgagtttgt tgagaaagtg cgtcgcgcgc tgtatctggg caaaatcgtt
     7621 tcctacgcgc agggcttctc tcaactgcgt gctgcgtctg acgagtataa ctgggatctg
     7681 aactacggtg aaatcgcgaa gatcttccgc gcgggttgca tcattcgtgc gcagttcctg
     7741 cagaaaatca ccgacgctta cgctgaaaat gcgggcatcg ccaacctgct gctggcgccg
     7801 tacttcaaaa atatcgctga tgaataccag caggcgctgc gtgacgtcgt ggcttacgcg
     7861 gtacagaacg gtattccggt accgaccttc tctgctgcag tggcctacta cgacagctac
     7921 cgcgcagcgg tactgccggc gaacctgatt caggctcagc gtgactactt cggtgcgcac
     7981 acctataaac gcactgataa agaaggtgtg ttccataccg aatggctgga ttaa
//
