LOCUS       MH325897               17663 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter werkmanii strain O24_G3521 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325897
VERSION     MH325897.1
KEYWORDS    .
SOURCE      Citrobacter werkmanii
  ORGANISM  Citrobacter werkmanii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Citrobacter werkmanii"
                     /mol_type="genomic DNA"
                     /strain="O24_G3521"
                     /db_xref="taxon:67827"
                     /country="Poland"
     misc_feature    1..17663
                     /note="antigen biosynthesis gene cluster"
     gene            1278..2567
                     /gene="wzx"
     CDS             1278..2567
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AWU66678.1"
                     /translation="MLVKLTKKINVNFEIKKIISNAGWLLFDKFVRLMFGLLVGVWVA
                     RYLGPETYGQLAYVITLISFFQVIALLGMDTILVRDIAHNIDKAHQNLGTALILRFCV
                     GFLCLIVAVGGVAFFNGAHFALLTLLAGGGLVFQCADTIDIWFQSQSQSKRTVIAKLC
                     AYTITNIMRVVLILCHSPLWAFALLLSIDALLSALALTYAYRKFRTKHQWSATVEKCK
                     LLLSESWPYLVSGLSIILYMRIDQVMIKNLLGEESLGVYAAALQFSTLWNFVPVTLSV
                     SLAPYIARKKREGEHEYYKAIGNVFLMFSFIGWGITLIISVMSPFIIEIMLGTNYTNG
                     IGVLAIHVVTNLFICLGVAQSLWIVNERKGAFTLYKSLTGLVVCGIANLTLIPYYGIK
                     GAAISAVLSQFSASILFNLFFAKRIFLMQLKSLFFLR"
     gene            2584..3495
                     /gene="gt1"
     CDS             2584..3495
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66679.1"
                     /translation="MKKYSPVIAFVYSRPEHTKKMLTALSKNSLAEYTDLIIYSDGAK
                     TQKDDAGVQAVREYIDTITGFNSITIVKQDKNIGLAQSIIQGVTETVNKFGRVIVLED
                     DIETSPYFLKYMNDALDMYAEVKKVGSISGSSYPVKMANNNESTYLLRIPLCWGWATW
                     ADRWQSFNKDINTVKLTDKKIKDYINFDGAFDFFEQATLNADGKLNTWFIFWYITLAS
                     NKWLALFPTTVMAKNIGHDGSGENCNGDDIYKDTAIANKPIELNRKDVLIESKDYYLA
                     HIDYFRRNKKSLLARVISFVKRKIKGA"
     gene            3497..4177
                     /gene="orf3"
     CDS             3497..4177
                     /gene="orf3"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AWU66680.1"
                     /translation="MGFLNRFKSYLIKRDINKNGMGIYIDLLSYVDEYSTFEGNNRIT
                     GKSSIYNSHIGRYSYAVGASIGNAMVGRFCSIAMGSKIGGLGAHPTSLISTHPIFYSS
                     RKQCGVSFTNEDKFAEEKTTILGNDVWVGANAIIMDGVKIGDGAIIAAGAVVTKDVLP
                     YAIVAGVPAVVKRFRCSAQHVDVLKDIEWWNWSETVLKDYLHLFQGDIKDNIVELIRV
                     SKKLKEHN"
     gene            4178..5338
                     /gene="wzy"
     CDS             4178..5338
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AWU66681.1"
                     /translation="MQFKSNNVYKSILLLSFFFSGFSLYSVFIPLLMLSLFLTVYKNK
                     SLYAGSVATSWNVLFLVLFVLCVFIIGYSRLYLDSPIKYAMYIVFMIVCLTLILVNNI
                     ENAKEMLLFLILGIFVRAEFVVIYSFLEPSGVYGYGKLLDPFSHEEINSPGISNSLAI
                     VFMYLLVAFKANTFNKRIFLTLLYPLLLLSSIFLGGRTFFIIAIISLFYQYSRNINLK
                     ATIWFCLISIGALITFSLIFKDLPVFDRYFNLVFERFGSEGLKSARFELIQDGITKFL
                     MQPMGGFTPHASEYSGNWYHNIYLDSARVAGFIPLIMFLFSNFILFFSYLKYKKQNGK
                     FKSPYFFLSVLTLIVMCQDVVLEGNMMLLICYALFTTMHIQTGRYNKNGSIL"
     gene            5322..6464
                     /gene="gt2"
     CDS             5322..6464
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66682.1"
                     /translation="MEVYYRVNDYYPLGAEGSSAASSVRHRVDRVCRCLPDGSELKIY
                     SIIKSRNVSIVKREKHNISNTQQCITYCVASNFLGAILKGFISIFLMFYLIFKIMGRG
                     KYVLIYNLLPANILVYILPTYIFPWILKKTIFQVEEIYSVYDFSFLKRFLTQCSENFV
                     FKKGHNFIVVNENVRNLIKRKDANSIVDYGYNSRNGNTIKPSSTPGNIIYTGRLDFVG
                     GIEVLLQALTDLPTDIKNKLVITGNGGLKEHVVTCTNELGISYRGFLNESDYNSLLLD
                     AKISINPLRSQCKFSFYSFPSKVLQYLEYGCVVISSGISQPELEEYFADELLTYENDD
                     PVKLNSLIREANLLNFDKMTIINKYNVFMEMKEHKLRSFFTTVLGG"
     gene            6469..7128
                     /gene="orf6"
     CDS             6469..7128
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AWU66683.1"
                     /translation="MKLAVFDICGTLYHDNTTYSFLRWLSKKNIIKYNKSIMNAPYIL
                     RALNVIYSSFSRKDVYRILQVKQLSDFSFDDINKWAREFVDELEENRIYSTNILMEEY
                     KKQGYKIVIASATIEPVARAIAQKLGVEYYSSTIGFLNNKCTGEINKDLLFLKKDTLL
                     HLLKKAETTIVVTDNYTDIELVLVSDHSHIIVRNDRDIEKWNRCLKANARKVDFIKKD
                     A"
     gene            7128..7925
                     /gene="orf7"
     CDS             7128..7925
                     /gene="orf7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AWU66684.1"
                     /translation="MNKYIIYFPFGYTFLSRIKTSSKFISFLLTIPLPCFLFFYFGLL
                     DVEARTLSLGIVFILTYSILYIFYENGYVENDIKTVKKEVNPTLRIVGELYNYIDDNY
                     YRIIIIRVFFYALSLYFLSFFTIGDVIVKVVFFSIATSVIYFFHNRTRNAMKVFTFLL
                     LSSSRFVFPLMVFSSYITTEKIEYIPLSLFIYTIPAFFIYSAKTFTIMQFVIGHENKY
                     KLIYYASTCIVFLILYYLVPSIDVLLLMAAILFYMLLLISLKKILNK"
     gene            7937..8737
                     /gene="gt3"
     CDS             7937..8737
                     /gene="gt3"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66685.1"
                     /translation="MSKVCCRLAGGLGNQIFQYGASLLISKKSTVPLIALDDSELKKY
                     KAVRKNELPIYFKLVSSSIKNHSILKLRLPRMKAIAKYSDRFVGDYNFNRVANKIIPA
                     QNYYLDGYFQSCLDQSLFDEMLEVLKKDYRYTNLGIQKSICAVHIRGGDFLTAKYSGI
                     ADVNYYLEQLAKVKELNVAERFVIVTDDKKYASDIANKMAIKYSFSEGNMLDDFLLLA
                     QSETKILSNSTFSIWASALGYQPGCIVFAPKKLTVQDSRNFILPGELS"
     gene            8761..9876
                     /gene="gmd"
     CDS             8761..9876
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 46-dehydratase"
                     /protein_id="AWU66686.1"
                     /translation="MKVALITGVTGQDGSYLAELLLEKGYEVHGIKRRASSFNTERVD
                     HIYQDPHSSNPKFHLHYGDLTDSSNLTRILKEVQPDEVYNLGAMSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLG
                     NMDSLRDWGHAKDYVKMQWMMLQQDEPEDFVIATGVQYSVRQFVEMAAAQLGIELRFE
                     GKGVEEKGIVVSVTGDDAPGVKVGDVIIAVDPRYFRPAEVETLLGDPSKAHEKLGWKP
                     EITLSEMVSEMVAKDLEAAKKHSLLKSHGFNVSLSQE"
     gene            9880..10845
                     /gene="fcl"
     CDS             9880..10845
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AWU66687.1"
                     /translation="MNRQRIFIAGHRGMVGSAIVRQLEQRGDVDLVLRSRDELNLLDS
                     KAVLDFFAAERIDQVYLAAAKVGGIVANNTYPADFIFENMMIESNIIHAAHLHDVNKL
                     LFLGSSCIYPKMAKQPMAESELLQGSLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATMQNASEVIVWGSGTPMREFLHVDDMA
                     AASIHVMELDREVWQENTQPMLSHINVGTGVDCSIRELAQTIARVVGYRGQVLFDASK
                     PDGTPRKLLDVSRLKKLGWQFGISLEDGLGSTYHWFLENQHRFRG"
     gene            10848..11348
                     /gene="gmm"
     CDS             10848..11348
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AWU66688.1"
                     /translation="MFLSQDDFVTVVRSTPLVSIDLIVENTQGEFLLGKRLNRPAQGY
                     WFVPGGRVQKDEPLSAAFERLTEGELGLRLPLSAGKFYGVWQHFYNDNFANEEFSTHY
                     VVLGFRLQVQEKDLCLPDAQHEAYCWLAPAVLLSSENVHENSRAYFQNDANHVIGLET
                     KDVKYV"
     gene            11341..12789
                     /gene="manC"
     CDS             11341..12789
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AWU66689.1"
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSRELYPKQFLQLSGEHSLLQTT
                     LLRLSNLSCETPLVITNEQHRFIVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFSA
                     LKRNEQEDPLLLVLAADHIITKEKIFCDAIQKAIPIAENGSIVTFGIVPEYAETGYGY
                     IERDTDVLSLSNVAEGEFYRVKNFVEKPDRLTAEKYISSGNYFWNSGMFMFKASAYLE
                     ELKKYRPDIYSICEQVVSSSYRDLDFIRLPEEIFKDCPAESIDFAVMEKTDRCIVCPV
                     DIGWSDVGSWQSLWDISDKTHTGDVCKGDVLTYNTKNNYIYSESALVAAVGVEDVVIV
                     QTKDAILVSKKSDVQDVKKIVEMLKSQERSEYIAHREVFRPWGKFDAIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNDKTMLVTANESIYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYRKDD"
     gene            12793..14160
                     /gene="manB"
     CDS             12793..14160
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AWU66690.1"
                     /translation="MKKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANNFPAVDESKRGRYQRINPRDAYIDHLL
                     GYINPGNLKPMKLVLNSGNGAAGPVVDAIEARFKALGVPVEFIKIHNTPDGNFPNGIP
                     NPLLPECRADTRNAVIEHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGKPVMSKTGHAFIKERMRHEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELLCLKGQSLGELVRDRMAAYPASGEINSTLAEPAAAIAR
                     VEQHFSREALTVDRTDGISMSFVDWRFNLRSSNTEPVVRLNVESRGDEQLMRSKTEEI
                     LRILK"
     gene            14237..15049
                     /gene="gt4"
     CDS             14237..15049
                     /gene="gt4"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66691.1"
                     /translation="MNHSIAIILSVYNSEKPDYLHQSLSSLFSQTMKADIYLYIDGRV
                     NKSLESVIESFETEPNFYVVRGEENKGLAFALNTLIDVALIRGYRYIARMDTDDVSRL
                     NRIEIQFRFMEERPDIDVLGGYCHEFGSEYALELKKVPLEHDDLKTYSIIRCPFIHPT
                     VMFRSSVFKDGIRYPLDTKFTEDMALWFCLLEKGYRFHNIPEVLLDYRLNDSTFSRRR
                     GIGKAISEFSVRFKYMFKLKEASFKRFIILIIKFFFHVLPLFLIRYAYKRHR"
     gene            15092..16135
                     /gene="orf15"
     CDS             15092..16135
                     /gene="orf15"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AWU66692.1"
                     /translation="MKSIEHRNNCFDIIRLFAAITVIISHQYSLSGMSEPIFLGMSTY
                     GGVAVIVFFAISGFLISKSCMRSKGFVSYMLKRARRIFPALLPCAIFMYLVLGVYLKW
                     GDLSSYINISILKNIISVVTIQGAPPHTGIFEGFIHPYSLNDSLWTLPLEFLCYLLLG
                     MVFFLFKNSLKAMLIAFVVLFIISIYLLFNKTAIVFFGVVLQAFPVRALSFFTGAIMA
                     LTINYWSDKKTKQYILIISFTILFSVAYKNEINVLGYVLLSTITIVIGCSFKDRIISG
                     RFDYSYGIYIYSFPVQQFIINKFHFEFYFGMFVSIIVSLFFGFLSWHFVESKFVKVNK
                     SYVKKHDVLGQST"
BASE COUNT     5091 a   3100 c   3961 g   5511 t
ORIGIN
        1 atgactaatt tgaaagcagt tattccggta gcgggcctcg ggatgcacat gttgcctgcc
       61 acgaaggcaa tcccgaaaga gatgctgcca atcgtagaca agccgatgat tcaatacatt
      121 gtcgacgaga ttgtagctgc tggcattaaa gaaattgttc tggtaacgca tgcgtccaag
      181 aacgcagttg agaaccactt cgacacctct tacgaactcg aatcgttgct tgagcagcgt
      241 gttaagcgcc agttattagc tgaggtgcag tccatctgtc ctcctggcgt cactatcatg
      301 aacgttcgtc aggcgcaatt actcggcctg ggccattcaa tcctttgcgc tcgcccgatt
      361 gttggggaca acccgttcgt cgtggtgctc ccggatgtcg ttcttgatac cgcaagtgcg
      421 gaccctctgc gttacaacct ggctgccatg gttgctcgtt ttaatgaaac cgggcgtagt
      481 caggtgctgg ccaagcgcat ggaaggcgat ctgtctgagt actcggttat tcagaccaaa
      541 gagcctttag acaacgaagg taaagttagc cgtattgttg aattcatcga gaaacccgat
      601 caaccacaaa cgctggattc cgatctgatg gccgtagggc gctatgtgct ttcggctgat
      661 atttgggctg aattagagca tacacaacct ggcgcgtggg gacgtattca gttaacggat
      721 gcgattgcag aactggcgaa aaaacagtct gttgacgcca tgctgatgac gggtgacagt
      781 tacgattgtg gtaaaaaaat gggctacatg caggcttttg tgaagtacgg gctgcgtaat
      841 ctgaaggaag gtgccaagtt ccgtaagggc atcgagaagc tactgagcga gtagagactt
      901 acaggtcttt gtgacggtaa gccagaaaaa atagcggcag ctaacatcct tgtgactatg
      961 ctttatgctg tggggtgtta ctgtcgtttt tttatgaaaa acaaccaata acaacgagtt
     1021 aacctactaa gtttaatcct ggattgggtt gatttttcct tgtttctgat agcatttggt
     1081 aagacaatta gcgttaaaaa tttcagagct ttgcgatagc gctaacggag ttgatcggac
     1141 cttttagatt ttaagcagtg cactggtagc tgtcgagcca ggggcggtag catgcctatt
     1201 agaatcagaa aacttataaa tctataaata ttgagtaggt ttattactta gattgcaaaa
     1261 tttcgcaggt gttttgaatg ctggttaaac ttacaaaaaa aataaatgtt aattttgaaa
     1321 ttaagaaaat aatttcaaat gccgggtggc ttttatttga taaattcgtc cgattaatgt
     1381 ttggtctgct tgtaggagta tgggtagctc gttatttggg gccagagaca tatggtcaac
     1441 tggcatatgt aattacgctc attagttttt ttcaagtgat agcattgctt ggaatggata
     1501 cgattttagt acgcgatatt gctcataata tagataaggc acatcaaaat ttaggaaccg
     1561 cgctcatttt gcgtttctgt gttggttttt tatgtttaat cgtggcagtg ggtggggttg
     1621 ctttttttaa tggtgcccat tttgcattgt taaccttatt ggctggtggt ggactagtat
     1681 ttcaatgcgc tgatacaatt gatatttggt ttcaaagtca aagtcagagt aaaagaactg
     1741 ttatagcaaa gttgtgcgca tacactatta caaatataat gagagttgtt ctaatattat
     1801 gtcacagtcc gctctgggca ttcgcgttac tacttagcat agatgcgtta ttatctgcgt
     1861 tagcgctgac ctatgcgtat agaaaattta gaactaaaca tcagtggtct gcaactgtag
     1921 aaaaatgcaa attattactt agtgagtctt ggccttattt agtaagtgga ctatcgatca
     1981 tactatatat gaggatagat caggtcatga taaaaaatct attaggtgaa gaatcattag
     2041 gggtttatgc ggccgcattg caattttcga cattatggaa ttttgttccg gtaacattat
     2101 cagtgagttt ggcaccatat attgcaagaa agaagagaga gggtgagcat gagtattata
     2161 aagctatcgg aaatgttttt ctgatgtttt ccttcattgg ttggggaata acgttaataa
     2221 tttcagtaat gtctccattt attatagaaa ttatgctagg aaccaattat accaacggaa
     2281 ttggagtgct tgctatacat gtagtgacaa acttattcat ttgcttaggg gtagctcaaa
     2341 gcttatggat tgttaatgaa aggaaaggag catttactct atataagtcg cttacaggtc
     2401 ttgtcgtttg cggcatagca aatttaactt taattccata ctatggaatt aaaggtgccg
     2461 cgatatctgc agtattgtcc cagttttcgg catctatact atttaatttg ttttttgcta
     2521 aaagaatttt tttgatgcaa ctcaaaagtt tatttttttt aaggtaataa acaggaacgg
     2581 aaagtgaaaa aatattctcc tgtaatcgct tttgtctatt cacgacctga gcatacaaaa
     2641 aaaatgttaa cggcattaag taagaatagt cttgcagaat atactgacct tattatctat
     2701 tcagacggtg caaaaaccca gaaggatgat gccggtgtcc aggctgtgcg agaatatatt
     2761 gacactataa ctggattcaa tagtataacg atagttaagc aagataaaaa tattggactt
     2821 gctcaaagta taattcaggg cgtaacggaa acagtaaata agtttgggcg cgtaattgtt
     2881 cttgaggatg atatagagac atcaccttat ttcttaaagt atatgaatga cgcattagat
     2941 atgtacgcgg aagtaaaaaa ggtaggctct atttccggta gcagctatcc tgtcaagatg
     3001 gccaataata atgaatcaac ctacctacta agaattccgc tgtgctgggg atgggctaca
     3061 tgggcagatc gatggcagag ctttaataaa gatattaata cagttaaact aactgataag
     3121 aaaattaaag attacataaa ttttgatgga gcatttgatt tttttgaaca agctacgctt
     3181 aatgctgatg gtaaacttaa tacatggttt atcttctggt atataacgct agcatcaaat
     3241 aagtggttgg ctctttttcc gacaacagta atggcgaaga acataggtca cgatggttca
     3301 ggggaaaact gtaacggtga tgacatttat aaagatactg ctattgctaa taaacccata
     3361 gagttaaacc gaaaagatgt tctaattgaa agtaaagatt attatttggc acatatagac
     3421 tatttcagaa gaaacaaaaa aagcctactt gccagagtta tttcttttgt aaaaagaaag
     3481 ataaaagggg cttgatatgg gatttttgaa tagatttaaa tcatatctga taaaaagaga
     3541 cataaataaa aatggtatgg ggatttatat agacctgcta tcttatgttg atgaatattc
     3601 cacgtttgaa ggtaataata gaattactgg aaaatcctca atttataact cacatattgg
     3661 gagatattcc tatgccgttg gagcaagcat aggaaatgct atggtgggca gattttgcag
     3721 tattgctatg ggttcaaaaa ttggagggct tggcgctcat ccaacgtctt taatatcaac
     3781 acatccaatt ttttattctt caagaaaaca atgtggtgtc tcgtttacaa acgaagataa
     3841 atttgcagaa gaaaaaacaa ctattttagg taatgatgtt tgggttggtg ccaatgctat
     3901 tattatggat ggtgttaaaa taggcgatgg tgcaatcata gcagcaggtg ctgttgtaac
     3961 aaaagatgtg ctgccgtatg cgattgttgc tggtgttcct gctgtcgtta aacgattccg
     4021 ctgttctgca cagcatgtag atgtattaaa ggatatagaa tggtggaatt ggtctgagac
     4081 tgttttaaaa gattatttac atttattcca gggggatatt aaagataata tagttgaatt
     4141 aattagagta agtaaaaaac taaaggaaca taattgaatg caattcaaaa gtaataatgt
     4201 atataaaagc atactgttat taagtttttt cttctctggt tttagcttat attctgtatt
     4261 tataccttta ctaatgttgt cactattcct aacagtctat aaaaataaat cattatacgc
     4321 aggaagtgtc gcaacaagtt ggaatgtatt attccttgtt ttatttgtgt tatgtgtttt
     4381 tattattgga tactctaggc tatatcttga ctcgcccata aagtatgcaa tgtatattgt
     4441 ctttatgata gtctgtttaa cgctaatatt agttaataat attgagaatg ctaaggaaat
     4501 gttgcttttt cttattctcg gtatttttgt tagagcagaa tttgttgtta tttatagttt
     4561 tcttgaacct tctggagttt atggatacgg taaattgctg gatccattta gtcatgagga
     4621 aattaactcg cccggaataa gtaactcact agctattgtg tttatgtatt tattagttgc
     4681 gtttaaagct aatacgttta acaaaagaat ttttctgaca ttattatatc ctctgctttt
     4741 attatcatcg atattccttg gtggacgtac gttttttatt attgcaatta ttagtttgtt
     4801 ttatcaatac agtagaaata taaatctgaa agcaaccatt tggttttgtt taataagcat
     4861 tggtgcccta attacgtttt ctctaatatt taaagatttg ccagtttttg ataggtattt
     4921 caatttggtg tttgaacggt ttggaagtga agggctgaaa tcagctagat ttgagcttat
     4981 acaagatgga atcacaaaat tcctgatgca acctatgggt ggatttacac cgcatgcaag
     5041 tgagtattct ggaaattggt atcataacat ttatttagat agcgccagag ttgccggatt
     5101 tatacccctg atcatgttcc ttttttctaa ctttattctt ttctttagct atttaaaata
     5161 taaaaagcaa aatgggaaat ttaagagtcc gtatttcttt ttgtcggttt taacactaat
     5221 agtaatgtgt caggatgtag ttcttgaggg taatatgatg ttgctgatct gttatgctct
     5281 atttacgact atgcatattc agaccgggag gtataataaa aatggaagta tattatagag
     5341 taaatgatta ttatcctttg ggtgctgaag ggagctctgc tgcgagttcg gtgcggcata
     5401 gagttgatag agtttgccga tgtcttccag atggaagtga acttaaaata tattcaatta
     5461 ttaaatctag aaatgtatca atcgtaaaac gcgaaaaaca taatatttcg aatacgcaac
     5521 agtgtataac atattgtgtt gcctctaatt ttttaggtgc aatattaaaa gggtttattt
     5581 cgattttttt aatgttctat cttatattta aaataatggg tagagggaag tatgtcctga
     5641 tctacaattt attacctgct aatatccttg tttatatatt acccacatat attttccctt
     5701 ggatattgaa gaaaactata tttcaagttg aggaaatata cagtgtctat gatttttctt
     5761 ttcttaagcg gtttttaacg cagtgttcgg aaaattttgt tttcaaaaaa gggcataatt
     5821 tcattgtcgt taatgaaaat gtcagaaatc taattaagag aaaagatgca aattcaatag
     5881 ttgactatgg atacaattct agaaatggta atacaattaa acctagctca acaccaggaa
     5941 atattattta tactgggaga ttagatttcg ttggtggtat agaagtactc ttacaggctc
     6001 tgactgattt acctacggat ataaaaaata agttagtaat taccggtaat gggggcttaa
     6061 aagaacatgt tgttacctgt acaaatgagc taggaatttc ttatcggggt tttcttaatg
     6121 aatctgatta taattcattg ctgttagatg caaaaataag cattaaccca cttcggtcgc
     6181 aatgtaaatt ctctttttat tcatttccat caaaagtact acagtacttg gagtatggtt
     6241 gtgttgttat ttcgagtggg atttcacaac ctgaattgga agaatatttt gcggatgagt
     6301 tattaactta tgaaaatgat gaccctgtaa aacttaactc tttaattaga gaagcaaatc
     6361 tgctgaattt tgataaaatg acaataataa ataagtataa tgtcttcatg gagatgaaag
     6421 aacataagct aaggtccttt ttcacgacag ttttaggagg atgatatttt gaaattggct
     6481 gtttttgata tatgcggtac gttataccat gacaatacta catattcatt tttgcgttgg
     6541 ctttctaaaa aaaacataat aaagtataac aagagtataa tgaatgcccc atatatattg
     6601 cgtgcgctga atgtaatata ttcatccttc agtaggaagg atgtttatag gattttacag
     6661 gtaaaacaat tatctgattt ctcattcgat gatatcaata aatgggcaag ggaattcgta
     6721 gatgaactag aagagaatcg catatatagc acaaatattt tgatggagga atataagaaa
     6781 caagggtata aaattgtgat cgcaagtgca actatcgaac ctgtagcaag ggcaattgct
     6841 caaaaactag gtgtcgagta ttattcttca acaattggtt ttttaaataa taagtgcaca
     6901 ggtgaaatta ataaggatct gttattttta aagaaagaca ctttgttgca tttattaaaa
     6961 aaagcagaaa ccacaatagt cgttactgat aactacacag atatagagtt agttttagtg
     7021 agcgatcatt ctcatattat tgtacgcaat gatagagata tagaaaaatg gaataggtgt
     7081 cttaaagcaa atgctcgcaa agttgatttt attaagaagg atgcttaatg aataaatata
     7141 ttatatattt tccattcgga tatactttct tatctcgtat taagacctct tctaaattta
     7201 ttagtttttt actcacaata cctttacctt gttttttgtt cttttatttt gggttgctag
     7261 atgttgaggc gagaacctta tctctgggga tcgtttttat tttgacatac tccattttat
     7321 atatttttta tgagaatgga tatgtagaaa atgatattaa aacggtaaaa aaggaagtga
     7381 atccaacatt aagaattgtt ggtgagttgt ataattacat agacgataac tattacagaa
     7441 ttatcatcat tagagttttt ttctatgctc tatcacttta ctttttgtct ttttttacta
     7501 ttggcgatgt cattgtaaaa gttgtttttt tctctattgc aaccagtgtg atttattttt
     7561 ttcacaatag aactcgaaat gcgatgaagg tatttacttt cttgttatta tcctcatcgc
     7621 gatttgtttt tccactgatg gtcttttcat cttatataac cacggaaaag atcgaatata
     7681 taccattgtc actttttata tatacaatcc cagcattctt tatatattca gccaagacat
     7741 ttacaataat gcaattcgta attgggcacg aaaataaata caaattaata tactatgcat
     7801 ctacgtgtat tgtttttttg attttgtatt atttggtgcc aagtattgac gttttattgt
     7861 taatggctgc tatacttttt tatatgttat tacttatttc tttaaaaaag atattaaata
     7921 aataaatgga gtgtcaatgt ctaaagtatg ttgcagatta gcaggtgggc taggcaatca
     7981 gatctttcaa tatggcgctt cacttcttat tagcaaaaaa agtacggttc cattgattgc
     8041 acttgatgat tctgagttaa aaaaatataa ggcagtaagg aaaaatgaat taccaattta
     8101 ttttaaattg gttagttcat cgataaagaa tcatagtatt ctcaagttgc gtttaccccg
     8161 aatgaaagca attgcaaaat acagtgatag gtttgttggt gactataatt ttaatcgagt
     8221 ggctaataaa ataatccctg cacaaaacta ttacttggat ggttatttcc aaagttgtct
     8281 tgaccaaagt cttttcgatg aaatgcttga ggttctgaaa aaagactacc gatacaccaa
     8341 tcttggaatt caaaagagca tttgcgcagt tcatattcgt ggtggtgatt ttttaactgc
     8401 aaaatacagt gggattgctg atgtaaatta ttatctggaa cagttggcta aggtcaaaga
     8461 gttgaatgtt gcagagcgat ttgttatcgt aacagatgat aaaaaatatg cttctgatat
     8521 agccaataaa atggctataa aatattcatt ttccgaaggc aatatgcttg atgatttttt
     8581 attgttagca caatctgaaa ctaagatatt atctaatagt acgttttcta tttgggcatc
     8641 agctttaggc taccaacctg ggtgcattgt ttttgccccc aagaagttaa cggttcagga
     8701 ctctagaaat ttcattcttc ctggtgagtt aagttaataa tataaggagt tatttaaaaa
     8761 atgaaagttg ctttaattac tggcgtgact gggcaggatg gttcatattt ggcggagctt
     8821 ttgctggaaa aaggttatga agttcatggc attaagcgtc gtgcttcttc gtttaatact
     8881 gaacgcgtgg atcacattta tcaagaccca cattccagca acccgaaatt tcatttgcat
     8941 tacggcgatc tgacagactc ttctaacctt actcgtattc tgaaagaagt tcagccggat
     9001 gaagtctata accttggtgc aatgagtcac gtagcagttt cctttgagtc tccggaatac
     9061 actgctgatg tcgatgctat ggggacattg cgtctattgg aagccattcg tttccttggt
     9121 ctggaaaaga agacccgttt ctatcaagca tccacttctg aactttacgg cctagtgcaa
     9181 gaaattccgc aaaaagagac caccccgttc tacccgcgct ctccgtatgc tgttgctaaa
     9241 ctgtatgcct actggattac agtgaattac cgtgaatcat atggcattta tgcctgtaac
     9301 ggtattctat tcaaccacga gtcgccgcgt cgtggcgaaa cctttgtgac ccgtaaaatt
     9361 acccgtgcta ttgctaacat cgcacaaggt ctagagtcct gcttgtatct tggcaatatg
     9421 gattctctgc gcgactgggg acatgcgaag gactacgtaa agatgcagtg gatgatgctg
     9481 caacaggatg aaccggaaga ctttgttatt gctactggtg tgcagtactc cgtacgtcag
     9541 tttgttgaaa tggccgcagc ccagttggga atcgaattac gttttgaagg taaaggtgtc
     9601 gaggagaagg ggatcgtggt ttcagttacc ggcgatgatg ctcccggcgt gaaggtggga
     9661 gatgtaatta ttgctgtaga tccgcgctat ttccgtcctg cagaagtaga aactctgcta
     9721 ggtgatccga gcaaagcaca cgaaaaacta ggctggaaac cggaaatcac actttcggag
     9781 atggtatctg aaatggttgc gaaagatctg gaggcagcga agaaacactc tctgttaaag
     9841 tctcacggat ttaatgtcag tttatctcag gaataatcga tgaacagaca acgtattttt
     9901 attgctggtc atcgcggaat ggtgggttcc gcgattgttc gccagttaga acaacgcggt
     9961 gatgtggatc tggtgctgcg tagccgagat gaactgaact tgctcgacag taaggctgtt
    10021 ttagactttt ttgccgccga gcgtatagat caggtctatc tggctgcggc taaggtggga
    10081 ggcattgttg ccaacaacac ttacccagcc gatttcattt tcgaaaatat gatgatagag
    10141 agcaacatta ttcatgcggc gcatctgcac gacgtgaaca aattgttatt tctcggctct
    10201 tcctgtattt accctaagat ggcgaaacag ccaatggcgg agagtgaact gctgcagggc
    10261 tcgctcgaac caactaatga accatatgct attgctaaga tcgccggaat aaagctgtgt
    10321 gagtcctata accgccaata cggtcgtgat tatcgctcgg tgatgccgac aaacctatac
    10381 gggccccacg acaacttcca cccgagtaac tcgcatgtta tcccggcgct gttgcgtcgt
    10441 tttcatgaag cgacgatgca aaatgcctca gaggtgattg tgtggggaag cggcacgcca
    10501 atgcgtgagt tcttgcatgt agatgatatg gcagcagcca gtattcacgt tatggaattg
    10561 gaccgcgaag tttggcagga aaacacccag ccaatgctat ctcacattaa tgttggtaca
    10621 ggtgtggact gttccattcg tgagctagct caaacaatcg cacgagtggt gggctacaga
    10681 ggacaggtgc tatttgatgc gagcaaaccg gacggcactc cccgcaaatt acttgacgtc
    10741 tcgagactaa aaaaattggg ctggcagttc ggcatttcac tcgaagatgg cctgggcagc
    10801 acatatcatt ggttccttga gaatcaacat cgattccggg ggtaagtatg tttttgtctc
    10861 aggacgattt tgtcaccgtc gttcgttcta ctccgcttgt ctccatcgat ttgattgtgg
    10921 aaaacacgca gggcgaattt cttttaggta aacggctgaa ccgtcccgcg caaggctatt
    10981 ggtttgtacc tggtggccga gtacagaagg atgagccatt gagtgcggcc tttgaacggc
    11041 taactgaagg cgaattggga ttgcgattac cgttgtccgc tggcaagttt tatggcgtct
    11101 ggcaacactt ctataacgac aatttcgcca atgaggagtt ttcgacccat tacgtggtgc
    11161 tcggttttcg tctgcaagta caggaaaaag acctgtgttt gcccgacgcg cagcacgaag
    11221 catactgctg gctggcgcct gcggtattgt tgagtagcga gaacgttcat gaaaacagtc
    11281 gtgcctattt tcaaaatgat gccaatcatg tcattggctt agaaacgaag gatgtcaaat
    11341 atgtctgatg taccgttaat cgctgtcgta atggctggtg gtacggggag tcgtctttgg
    11401 cctctatcta gggagcttta tccgaaacaa tttttacaac tttcgggtga acactcccta
    11461 ttacagacaa ccttacttcg gctgtcgaat ctttcttgtg aaacgccatt agttatcacg
    11521 aatgaacaac atcgctttat tgttgctgag cagttaagac agatagatca gttacgcgat
    11581 aatatcattc tggaaccgtg tggacgcaat actgcacctg caattgcgct ttcagctttt
    11641 tctgcgctga agcgtaatga gcaagaggat ccattgctat tggtacttgc tgctgaccac
    11701 attatcacta aagaaaaaat cttttgtgat gcaattcaga aagcaatacc aattgcagaa
    11761 aatggaagta ttgtgacctt cggtattgtg cctgaatatg ctgaaactgg ttatggctat
    11821 atagaaagag atactgatgt actatctctt tccaatgttg ctgaaggtga gttttatcgt
    11881 gttaagaatt ttgttgaaaa acccgatcgc ctaaccgctg aaaaatatat ttcatctgga
    11941 aattattttt ggaatagcgg tatgtttatg tttaaagcat cggcttattt agaagagtta
    12001 aagaagtata gaccagatat ctacagtatc tgtgagcagg tagtctcttc ctcttatcgt
    12061 gatcttgatt ttatacgttt accagaagaa atattcaagg attgccctgc tgaatctatt
    12121 gattttgccg ttatggaaaa aacggatcgt tgtattgttt gtccagttga tatcggttgg
    12181 agcgatgttg gctcttggca atcattatgg gatattagtg ataaaacaca tactggcgat
    12241 gtttgcaaag gagatgtcct gacatataac accaagaata attatattta ttcagagtcc
    12301 gcactggttg cagctgttgg tgttgaggat gtcgttatag ttcagacgaa agatgcaata
    12361 cttgtttcta agaaatctga cgttcaggat gtcaaaaaga tagttgaaat gctcaagtca
    12421 caagaacggt cagaatacat cgcacatcgt gaggtatttc gaccctgggg taaatttgat
    12481 gcaattgatc aaggcgaacg atataaagtt aagaaaatca tagtcaaacc gggtgaaggg
    12541 ctttctttac gtatgcatca tcatcggtct gaacactgga ttgtgctttc tgggacggcg
    12601 aaagttactc ttaatgataa aacgatgttg gtcactgcaa atgaatcaat ttatatccct
    12661 cttggggcca cgtatagtct agagaatcca ggaattatcc cccttaatct cattgaagta
    12721 agttcaggag attatttagg agaggatgac atcgttcggc aaaaagaacg ttatagaaaa
    12781 gatgattagg gtatgaaaaa attaacctgt ttcaaagcct acgatattcg cggcaggctg
    12841 ggcgaagagc tgaatgaaga tattgcgtgg cgcattggcc gcgcttacgg cgagttcctg
    12901 aagccaaaaa cgattgtcct cggcggggat gtgcgcctga ccagcgagac gctaaagctg
    12961 gcgctggcga aaggactgca ggatgcaggg gtggatgtgc tggatattgg tatgtcgggc
    13021 accgaagaga tttatttcgc cactttccat cttggcgttg atggcggtat cgaagtgacc
    13081 gccagccata atccaatgga ttacaacggc atgaagctgg tgcgggaagg ggcgcggccc
    13141 atcagtggcg ataccggtct gcgcgacgtg cagcgtctgg cggaagcgaa taacttcccg
    13201 gcggttgatg agagcaaacg tggccgttac cagcggatta atcctcgcga tgcctacatc
    13261 gaccatctgc tgggttacat caatcccggc aacctgaaac cgatgaagct ggtgctcaat
    13321 tccggcaacg gtgcggcagg tccggtggtt gacgccattg aggcccgctt taaggcgctg
    13381 ggcgtgccgg tcgagttcat caaaattcac aacacgccgg atggcaattt ccccaacggt
    13441 attcccaacc ctctgctgcc ggaatgccgg gctgacacgc gcaatgcggt gattgaacac
    13501 ggcgcggata tggggattgc cttcgacggg gattttgacc gctgcttcct gttcgatgaa
    13561 aaaggccagt tcattgaggg gtactacatt gtcggcctgc tggcggaagc atttctggag
    13621 aagaatccgg gggcgaaaat tatccacgac ccgcgcctgt cctggaatac cgtggatgtg
    13681 gtgacggcgg ctggcggtaa accggtgatg tcaaaaaccg gacacgcgtt tatcaaagag
    13741 cgtatgcgcc atgaagatgc catttacggc ggcgagatga gcgcgcatca ctattttcgc
    13801 gactttgctt actgcgacag cgggatgatc ccgtggctgc tggtggcgga actgctgtgc
    13861 ctgaaaggac agtcgctggg tgaactggtc cgtgaccgga tggcggcata cccggcgagt
    13921 ggagaaataa acagtacgct ggcagagcct gcagcagcga ttgcacgcgt ggagcagcac
    13981 ttttcccgtg aggcgctgac ggtggacaga acggatggca tcagcatgtc gtttgtcgac
    14041 tggcgtttca atttacgctc ttccaatacc gaaccggtgg tgcgcctgaa cgtggaatcg
    14101 cgcggcgatg agcaacttat gcgaagtaaa acagaagaaa tactcagaat tttaaagtaa
    14161 tgttcaagta taaggctgtg tcgaaatcat agcctttgtt ttttttaagg tttcatcaga
    14221 tggattattt cttaggatga atcatagcat agcaataatt ctgagtgttt ataattcaga
    14281 aaaaccagat tatttgcatc aaagtctttc tagccttttc tctcaaacta tgaaagcaga
    14341 tatttacctc tatattgatg ggagggttaa taagtctctt gaaagtgtga tcgagagttt
    14401 tgaaactgaa cctaatttct atgttgtgcg tggagaagaa aataaggggc tggcttttgc
    14461 gctgaataca cttatcgatg tggctttaat tcgaggttat cgatatattg caaggatgga
    14521 tacggatgat gtctcacgct taaaccgtat cgaaatacag tttaggttca tggaggaaag
    14581 acctgatatt gacgtactgg gcggatattg ccatgaattt ggtagtgagt acgcacttga
    14641 attgaagaaa gtaccgctcg aacatgatga tcttaaaaca tacagtataa tccgttgccc
    14701 atttatacat ccaacggtga tgtttagaag ttcagttttc aaagatggga ttcgttaccc
    14761 tttagataca aaatttactg aggatatggc gttgtggttt tgcctccttg aaaaaggata
    14821 tcgttttcat aatatccctg aggtgttgct ggactacagg cttaatgata gcactttttc
    14881 aagacgaaga ggaattggga aagcaataag tgagttctct gttcgcttca agtatatgtt
    14941 taaactaaaa gaggctagtt ttaagaggtt tattattctg attattaaat tcttttttca
    15001 tgttttacct ttgtttttaa ttcgctatgc ttataaaaga catcgctgag tctgtgatgg
    15061 atataaaaat ttataaaatg aagattgtaa aatgaaaagt atagaacatc gaaataattg
    15121 ttttgatata attagattgt ttgcagcaat tactgtcatt atctcccatc aatactctct
    15181 ttctgggatg tctgagccaa tttttcttgg catgagtacc tacggaggtg tggcggttat
    15241 tgtttttttt gctatctcgg ggttcttgat ttccaaatct tgcatgagaa gtaaagggtt
    15301 tgtttcatat atgttaaagc gtgcaaggag aatattccca gcgttattgc catgcgcgat
    15361 ttttatgtat ctagtgttag gtgtgtactt aaagtgggga gacttatctt cttatattaa
    15421 tatatcaatt ctgaaaaata ttattagcgt tgtaaccatc cagggtgcac cgcctcacac
    15481 aggtatattt gagggtttta ttcacccata ctctcttaac gatagtttat ggacattacc
    15541 acttgagttt ttatgttatc ttcttttggg gatggtgttt tttttattta aaaattcttt
    15601 aaaagcaatg ttgattgctt tcgtagtgct ctttattata tctatttatt tacttttcaa
    15661 caaaacagca atagtttttt ttggtgtggt tttacaagcg ttcccggtga gagctttatc
    15721 cttttttact ggagctataa tggctctcac gataaattat tggagtgata aaaaaacaaa
    15781 gcaatatata ttaataatat catttactat tcttttttct gtcgcttata aaaatgaaat
    15841 taatgtgttg gggtatgttt tattatcaac aattacgata gttattggct gttcatttaa
    15901 agatagaata atttctggtc gatttgatta ttcatatggg atatatatat attctttccc
    15961 tgtccaacaa tttattataa ataaatttca ctttgaattt tatttcggga tgtttgttag
    16021 tatcattgtg tcactttttt ttggattcct ttcatggcat tttgttgagt cgaaatttgt
    16081 taaagtgaat aaatcatatg tgaaaaaaca tgatgtgttg gggcagagca cttaatactt
    16141 acttaatata gtatttagtt tgcttctatc attttatatc tgagttgagt taacatctaa
    16201 gccacattca agccgcgcat atgtcgcgcg gtgaccacac ctgacaggag tatgtaatgt
    16261 ccaagcaaca gatcggcgtc gtcggtatgg cagtgatggg gcgcaacctg gcgctcaaca
    16321 tcgaaagccg tggttatacc gtctccatct tcaaccgttc ccgcgaaaaa actgaagaag
    16381 ttgttgccga gaacccgggt aagaaactgg ttccttatta cacggtgaaa gagttcgtcg
    16441 agtctcttga aaccccgcgt cgtatcctgt taatggtgaa agcgggcgct ggcaccgatg
    16501 ctgctatcga ttccctgaag ccgtaccttg ataaaggcga catcatcatt gatggcggca
    16561 acaccttctt ccaggacacc attcgtcgta atcgcgaact gtctgctgaa ggctttaact
    16621 tcatcggtac cggtgtttcc ggcggtgaag agggggccct taaaggtcct tccatcatgc
    16681 ctggtggcca gaaagaagcg tatgagctgg ttgcgccgat cctgactaaa atcgcagccg
    16741 ttgccgaaga tggtgagcct tgtgtgacct atatcggcgc tgacggcgca ggtcattacg
    16801 tgaaaatggt tcacaacggt attgaatacg gtgacatgca gctgatcgct gaagcctatt
    16861 ctctgctgaa aggcggtctg aacctgtcta acgaagaact ggctaccacc ttcaccgagt
    16921 ggaacgaagg tgagctgagc agctacctga tcgacatcac caaagacatc ttcaccaaaa
    16981 aagatgaaga aggtaaatac ctggttgacg tgattttgga tgaagcagcg aacaaaggca
    17041 ccggtaaatg gaccagccag agctctctgg atctgggcga accgctgtcg ctgatcaccg
    17101 agtctgtatt cgcgcgctac atctcttctc tgaaagacca gcgtgttgca gcatctaaag
    17161 tactgtctgg cccgcagact aagctggctg gcgataaagc ggagtttgtt gaaaaagtac
    17221 gtcgcgcgct gtatctgggc aaaatcgtct cctacgcgca gggcttctct caactgcgtg
    17281 ctgcgtctga tgagtacaac tgggatctga actacggcga aatcgcgaag atcttccgcg
    17341 caggatgcat cattcgtgca cagttcctgc agaaaatcac agacgcgtac gctgaaaaca
    17401 aaggcattgc caacctgctg ctggcaccgt actttaaaaa catcgccgac gagtaccagc
    17461 aggcgttgcg tgatgtcgtg gcctacgcgg tgcagaatgg tattccggta ccgacattct
    17521 ccgctgctgt agcctactac gacagctacc gttctgaggt actgccggcg aacctgattc
    17581 aggctcagcg tgactacttc ggtgcgcaca cctataaacg tactgataaa gaaggtgtgt
    17641 tccataccga atggctggat taa
//
