LOCUS       MH325898               13299 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter werkmanii strain O26_G3522 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325898
VERSION     MH325898.1
KEYWORDS    .
SOURCE      Citrobacter werkmanii
  ORGANISM  Citrobacter werkmanii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Citrobacter werkmanii"
                     /mol_type="genomic DNA"
                     /strain="O26_G3522"
                     /db_xref="taxon:67827"
                     /country="Poland"
     misc_feature    1..13299
                     /note="antigen biosynthesis gene cluster"
     gene            1263..2219
                     /gene="rmlD"
     CDS             1263..2219
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AWU66693.1"
                     /translation="MKLYLGIKSFSSGCIDGIEMRILLFGKNGQVGWELQRSLALLGE
                     VLALGRSDYFPCGDLSNLKGIADTIQRFRPDVIVNAAAYTAVDKAEQEQDLSWLINVR
                     AVEVMAIESKKLGALLIHYSSDYVFSGDGSSWQKEEDIAAPINHYGLAKLASEEVITK
                     FNPKSLIFRTSWVHSSRGANFIKTILNIAKSKKEISVVDDQFGSPTSAELIADCTAHV
                     IRKVLPDNNHYGIYHLVSSGYTTWFDYAQFIMNHIDSANYLLNKINNITSSEYNMLAR
                     RPLNSRLNNRKFQEQFNLLLPDWQFGVKRTLSEIEKACKEGK"
     gene            2219..2773
                     /gene="rmlC"
     CDS             2219..2773
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AWU66694.1"
                     /translation="MKITKTELNDVVIIEPSIFGDERGYFYESYNKQKFDNAIGEDII
                     FVQDNISLSQKGVLRGLHYQASPYSQGKLLSCLEGEIFDVVVDIRRNSPTFKKWIGVI
                     LNDINKKSIWIPKGFAHGFLTMSDLAKVCYKVTDFYNPNSERIIIWNDTDINVAWPEM
                     EHKIISEKDLLGVSLKLSELYDEQ"
     gene            2763..3686
                     /gene="gt1"
     CDS             2763..3686
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66695.1"
                     /translation="MNSSVAILVCTYNGEKYLKSQIDSIRAQTHNDWVIYISDDGSTD
                     NTRKIIEDCISEIGTERCKLIEGPRKGFARNFINGIKLIPKKYQYYAFCDQDDIWLNN
                     KIEKSIANLSCIIANIPAVYCSSTLLVDENENYIGPSSKITKPLSFKNSLIQCVAGGN
                     TMLFNLSAKLIIDKTPEDKIIPSHDWWVYILITACDGKVIYDPDPTLKYRQHYNNLVG
                     ENKTLSAKFKRVKILFNGEFKKSVDANLILLNHMHEILSQENKITAQRFFCLRKVSFL
                     SRIKQIIQLRLYRQSNIESLLFAVALFLKRI"
     gene            3751..4581
                     /gene="wzm"
     CDS             3751..4581
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="AWU66696.1"
                     /translation="MSLATKKSKNPIVFLKTVKNNRELIYELIKRDIASRYQGSVIGF
                     MWSFINPIIMLSVYTFVFSVVFKARWSADSTGSKTEFALILFSGLLVYNLFSECLNKS
                     PGLILSNVNYVKKVIFPLEILPFVVLGTAFFHMLISFVVWVVFYLIFFGIPHITIFLF
                     PLTLIPLIFLIIGLSLFLSALGVYLRDIGQIILLLTTVLMFMSPIFYPIQALPDNFKI
                     IMEFNPLTFIIEQVRAVIIFGNSIQWQKWGVWTIVSMIVTCLGFAWFQKTRGGFSDVL
                     "
     gene            4571..5911
                     /gene="wzt"
     CDS             4571..5911
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="AWU66697.1"
                     /translation="MSSEISISIENVSKVYEMYDNPRERLLQLFSSSKKYYKEFWALK
                     NINFHLRKGETVGILGRNGSGKSTLLQIIAGTLEATQGCVTTSGVVAALLELGSGFNP
                     DFTGRENIYLNGSILGFSREQMDNRIDEIIAFADIGQHLDQPVKTYSSGMAVRLAFAV
                     QACIEPQIFIVDEALAVGDEKFQRKCYDYIEKLRKKGCSILLVTHSTGTIEKFCQRAV
                     LLHKGEMHAIGSAKDVIDQYHALLYSDEQAYIRFVNSQKERESQSSLEDQTAETFKTE
                     TTDIVYGDSDSDSDGSSKKQSITRGLSAQIVKWGIFDSNGKQCELFNTGECITVTMQI
                     ESLSNINEIQAGLLLRTVEGVSVYGTSTRYHDSNLISVKCGDVWNCSFNIKLDLCPGS
                     YFITLAIAEVLEQSGMAYLDRKTDVMVIKVNQRTLKASGIASLETQVEVLKQKG"
     gene            5914..7491
                     /gene="gt2"
     CDS             5914..7491
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66698.1"
                     /translation="MNILVVTEVFLIGGLETNIAGEIRSLIKQGHSVYLAVGKEFDTS
                     LIPSEVTGIISNLDLNHNIGSEEFSNTINSLRKYIRHNNIELLHAQPFYSIIISAIVA
                     ELENKALVLTLHGPVSFANYLSPIFKFLLTDIILPNVNIAYVVSAEMKELSLAFSSES
                     NVQILPNGIELTQHDERSIKDERWVCVSRLDEQKIVGVLEFIKYAKAIGLPGVVIMGD
                     GHAKGTLEQEISAHNLSNYVEFAGYESDVRTAITSYSGVAGMGRVVLEGIAAKRRVIL
                     VGYDGVKAVLNPETFEKAAWCNFSGRNFDTITTEQLKCELSSISDKDLDSCYDIINDG
                     YNVDNIWLHFSSSVACILNNPFSSTDSFLRKIYSLITTGDVYFPRYENLYFSDAIYAL
                     ISQIIFNINNESVRQHFCLLEQKRNCEQLKQNNISIAEIQSIIELSQKNISINADRLY
                     GDFEKRINKLITYNDELIGVIDKANAQISNTKTLVLGLEEKILLLERKISEMDRKNDE
                     HMENSKGFLYRLTHREK"
     gene            7556..9547
                     /gene="orf7"
     CDS             7556..9547
                     /gene="orf7"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase"
                     /protein_id="AWU66699.1"
                     /translation="MNLQSISFYAKKSIKLIRDKGFKVFIKVAVAKVDRRLNGYNLIQ
                     QFPTIHSSDNYLNDSNFMEGELIIISGVPYDDIGGGQRAAQLARCALKTGKKVVYLYI
                     YKKYDFEKNIHVESDVSIHGLQHIYIGNTTPNIISKIVSANATLVIEHPHPDALPYLE
                     FFNNRGLKTVFELIDDWETSLGGDWFNLENYQRFVRESTVVVGTAKLLVKRLEDLGRE
                     DAIYLPNAANEYIFDKYRKYSKPFDMPSGYNRIALYFGSLYGEWFGWDYITAAAKANP
                     KIAFVLIGDKPTDRNISDNVFFLGAKDITELPAYLAHSDITLLPFVPGKISDAVSPIK
                     IFEYLFLGKPVIATQMPEIVDYPGVFIANDPVEFATLCKKVEVTAELISKNDIFISEN
                     SWFSRLDNILTSKTYERFHKKTSAVILIHNNANIIIRNLQSLIHHCDYFLKEIIVVDN
                     MSIDGGAELVASQFPNVKIIKNDVNGCSSGRNLGAKYATGQYLAFFDSDQWFTSFSSF
                     TEALNLLEKNANIGIIGWGAGWFDVNRSDLGGMISDYCPNRGMNAEAIHKGYRSDIGY
                     LGTCGVFMSRTIFEASKGFDTFYDPTCFEDTDLCFQIKALGFDVCYRDLTGIRHQPHQ
                     TTKADSGSDYYEKLFNRNANYFKEKWSSYPEFYVDYKGN"
     gene            9551..10651
                     /gene="mnaA"
     CDS             9551..10651
                     /gene="mnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="AWU66700.1"
                     /translation="MKKIICVVGTRPEAIKMAPLIQELKKSELFSVTVLATAQHRDLL
                     DQALELFGIAADDDLNVMTPGQTLQELTARLMIGVDRILEERKPDMVIAQGDTTTVFV
                     TALACFYKKIPFAHLEAGLRTNDLYSPFPEEANRILAGHLAALHFSPTSVSRDNLLKE
                     NIGEEKVFVTGNTVIDALLDTAKKDFDFGIDIPPNHRLVLVTSHRRENFGKPIENICA
                     ALLELVNKYPDIYVLFPVHPNPNVRNVTNSVLKGHNRIALVDPLDYGPFVSAIKKSYL
                     ILTDSGGVQEEAPALGKPVLVLRRDTERPEAVTEGVVKLVGTETVDIIREASILLSDS
                     IEYFKMAKGASPYGDGKASSRILNIIKEYFKV"
     gene            10656..11729
                     /gene="gt4"
     CDS             10656..11729
                     /gene="gt4"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66701.1"
                     /translation="MKMVYLSPVPWSSITQRPHFFVDAALRNDVESVLWIDPYPTRFP
                     NIGDFHPGRHAPEPVGMGLPKGLNILKPDFILPCEPLPIVFKLLNSGAINKIFQNVGD
                     FIDSDTLLVVGKPSLLALKLIDSFKWKKKWFDAMDDFPQFYVGLSKFNMIKTENKIIC
                     SVDEIICSSHALLEKFSLPDKTKLILNACSDTIERSSDSNTDNKFVFGYIGTIAQWFD
                     WTWVVELSKLNPDSLIRIIGPIKTSVPTNLPKNIKLEMAIEHSKVMSTIATFDVAIIP
                     FMLNEITKCVDPVKFYEYLLARKDIVSTAFGEMNYHHHSYVKNDVTINYKLPSGSLFF
                     PFSSMSPAPSVPRWNDRFQTLFI"
BASE COUNT     4112 a   2142 c   2772 g   4273 t
ORIGIN
        1 atgattaatt tgaaagcagt tattccggta gcaggtctgg ggatgcacat gttgcctgcc
       61 acgaaggcaa tccctaaaga gatgctaccg atcgtagaca agccgatgat tcaatacatt
      121 gtcgacgaaa ttgtagctgc aggtatcaaa gaaatcgtgc tggtgactca tgcgtctaag
      181 aacgcggttg agaaccactt cgacacctct tacgaactcg aatcgttgct tgagcagcgt
      241 gttaagcgcc agctattagc cgaggtgcag tctatctgtc ctccaggcgt gactattatg
      301 aacgttcgtc aggctcagtt actgggtctg ggtcattcaa ttctttgtgc tcgcccaatt
      361 gttggggata accctttcgt cgtcgtatta cctgatgtgg ttcttgatac tgcgagcgcg
      421 gatccgctgc gctacaacct ggctgccatg gttgctcgtt ttaatgaaac cgggcgtagt
      481 caggtgctgg ctaagcgtat ggaaggcgat ctgtctgagt actctgttat tcagaccaaa
      541 gagcctttag ataacgaagg taaagtcagc cgtatcgttg aattcattga aaaaccagac
      601 cagccgcaga ctctggattc cgatctgatg gcagtagggc gctacgtgct ttctgctgat
      661 atctgggcag aactggagca tacccagcct ggtgcctggg ggcgtattca attgacggat
      721 gctattgctg agctggctaa aaaacagtcg gtagatgcga tgctgatgac gggcgacagc
      781 tacgactgcg ggaagaaaat ggggtatatg caggcgtttg ttaagtacgg cctgcgtaac
      841 ctgaaagaag ggccgaagtt ccgtaagagc attgagaagt tgctcggaga atagaaacag
      901 gctgcagcaa cgacattgga aggaaagtcg cggtaactga ctacctcata tcgacatttg
      961 ataaggttgg gcgttagtgc cgctttttac ttagaacaag ctaacaatat agctttaacc
     1021 tactaagttt aatcttgctg ttgtcggatt ttttcttgtt tctgactaca tttggtaaga
     1081 caattagcga ttgattttta ggttttctgc gagtatttgc agaagctaac gtccaatttg
     1141 tttatacgta gtgcactggt agctgttgag ccaggggcgg tagcgttatc aaatgccata
     1201 gtttattcaa catatgaaaa gcattggaat tgattggttg acttttcaat agccttgatt
     1261 atatgaagct gtatcttggt attaaaagct tttcatctgg ttgtattgat gggatagaaa
     1321 tgagaatttt actatttggg aaaaatggtc aagtaggatg ggaactgcag cgctcacttg
     1381 ctttattggg tgaagtatta gcattaggcc gaagtgacta ttttccgtgc ggtgatttga
     1441 gcaatttgaa aggcatagct gatacgatac aacgatttcg cccagatgtt attgttaatg
     1501 ctgccgcata tacagctgtc gataaagccg aacaagagca agatttaagc tggttgataa
     1561 atgttcgggc cgtagaagta atggcaatcg agtcgaaaaa gttaggcgca ttattgatac
     1621 actattcatc tgattatgtt ttttcgggtg atgggagttc atggcaaaaa gaagaagata
     1681 ttgctgcgcc aataaatcac tatggtttgg ctaaactagc cagtgaagag gtaattacta
     1741 aatttaaccc aaaaagtcta atatttcgaa caagttgggt tcatagctcg cgcggtgcta
     1801 attttattaa aacgatatta aatattgcta aaagtaaaaa agaaatatca gttgttgatg
     1861 atcaatttgg ttctccaact tctgctgaat taattgcaga ttgcactgca catgttattc
     1921 gtaaagtgtt accagataat aatcactatg ggatatacca tttggtgtcg agtggatata
     1981 caacatggtt tgattatgct cagtttatta tgaatcatat tgatagtgca aattatctac
     2041 tgaataaaat aaataacatt acttcaagtg aatataatat gttagcgaga agaccattaa
     2101 actctagatt aaataacaga aaattccaag aacagtttaa cttacttctt cccgattggc
     2161 agtttggtgt taaaagaaca ctttctgaaa ttgaaaaagc ttgtaaagaa ggcaaataat
     2221 gaaaattaca aaaacggaat tgaatgatgt agtaattatt gaacctagca tttttgggga
     2281 tgaacgtggg tatttttatg agagctataa taaacaaaaa tttgataatg caattggtga
     2341 agatattata tttgttcaag acaacatttc attgtcacag aaaggggtat tgaggggatt
     2401 acattatcaa gcatcaccgt attcgcaagg taagctttta agttgtctgg aaggtgagat
     2461 tttcgatgtg gttgtagata taagaagaaa ctcaccaaca tttaaaaagt ggattggcgt
     2521 aattctcaac gacattaaca aaaagagtat atggatccca aaagggtttg cccatggttt
     2581 tttaacaatg tcagatttag caaaagtttg ttataaagtt actgattttt acaatccaaa
     2641 ttccgaaaga attattattt ggaatgatac tgatatcaat gttgcttggc cagaaatgga
     2701 acataagata atctcagaga aagatttatt aggcgtttct ctgaaattat cggagttgta
     2761 tgatgaacag tagtgttgct attttagttt gcacatataa tggtgaaaaa tatttaaaat
     2821 cacaaattga ttctatccgt gctcaaactc acaatgattg ggttatatat atttctgacg
     2881 atggctcaac ggataatact agaaaaataa tagaagattg cataagtgag attggtactg
     2941 aaagatgtaa attaattgaa ggaccacgta agggatttgc taggaatttt attaatggga
     3001 taaagttgat cccaaaaaaa taccaatatt atgctttttg tgatcaagat gatatatggc
     3061 taaataataa aatagagaaa agtattgcaa atctctcttg tatcatagca aacataccgg
     3121 ccgtatattg ctctagcaca ttattagtgg atgagaatga aaactatatc ggtccatcct
     3181 caaaaataac gaaaccttta tcttttaaaa attccttaat tcaatgtgta gctggtggaa
     3241 acactatgct gttcaattta tcggctaagt taatcattga taaaacacct gaagataaaa
     3301 ttattccatc ccatgattgg tgggtatata tcttaattac agcatgtgat ggaaaagtta
     3361 tatatgatcc cgatcccaca ttaaaataca ggcagcatta caataactta gttggtgaaa
     3421 acaaaacatt atcagctaaa tttaagcgag ttaaaatttt atttaatggc gaatttaaga
     3481 aaagcgttga tgctaacctt attttactta atcatatgca tgaaatactt agccaagaga
     3541 ataaaattac agcgcagcga tttttttgcc ttaggaaggt aagttttctt agcagaataa
     3601 aacaaattat acaacttcga ttatacaggc aaagtaatat tgagtctctc ttgtttgcgg
     3661 ttgctttatt tttgaagcgt atctaaatga tttgatcagt tatttatgtt aagaaagaag
     3721 attatattga atgtgtccat cgaggggaaa atgagtttag caacaaagaa atctaagaat
     3781 cctatagtgt ttctgaagac agttaaaaac aatagagaac tgatctatga attaattaag
     3841 cgagatattg caagtagata tcaaggttca gttatcggtt ttatgtggtc atttataaat
     3901 ccaataatta tgttgtctgt atacactttt gtatttagtg ttgtgtttaa ggctcgttgg
     3961 tctgcagata gtacaggaag taaaactgaa tttgcattga tattattctc agggttgtta
     4021 gtttataatt tgttttcaga gtgtctgaat aaatctcctg ggcttattct ttcaaatgtg
     4081 aattatgtga agaaagtaat atttccattg gagatattac cttttgttgt attaggtact
     4141 gctttttttc atatgctaat tagctttgtc gtttgggttg ttttttattt aatattcttt
     4201 ggtatacctc acattacgat atttttattt ccgctaactt tgatcccgct tatttttttg
     4261 ataattggtc tttcattatt cctgtcagcg cttggtgtat acttacgaga tatcggacaa
     4321 ataatattat tattaacaac agtacttatg ttcatgtcac caatatttta tcctatacaa
     4381 gccttgcctg ataactttaa gattatcatg gaatttaatc cactaacatt cattatagaa
     4441 caggttcggg cagtgataat attcggcaat agtattcaat ggcaaaaatg gggtgtatgg
     4501 acaattgttt caatgattgt tacatgcctt ggattcgcgt ggttccaaaa aacaagagga
     4561 ggattctctg atgtcctctg aaatttctat ttcaattgaa aatgtctcaa aagtttatga
     4621 gatgtatgat aatcctcgcg aaagattact tcagctattt tcttcttcaa agaaatatta
     4681 caaagaattt tgggctctaa aaaatataaa tttccactta cgcaagggag aaacagtcgg
     4741 tattctggga cgaaatggta gtggtaaaag tacactacta cagataatcg ccggaacttt
     4801 agaggctact cagggttgcg tcactaccag tggggttgtt gctgcattat tggagttggg
     4861 gtcgggtttt aatccagatt ttaccggtcg tgaaaatatt tatctaaatg gatctatttt
     4921 aggtttttct cgtgagcaaa tggataaccg aatagatgag ataatagcct ttgctgatat
     4981 tggtcaacac ttagatcaac cggtaaaaac ttattctagt ggtatggccg ttaggttagc
     5041 atttgcagta caggcttgta ttgaacctca aatttttatt gtagatgaag cactggctgt
     5101 aggtgatgag aaatttcagc gtaaatgcta tgactatatt gaaaaattaa ggaaaaaagg
     5161 ttgtagtatt ttattagtta cgcattcaac tggtacaatt gaaaagtttt gccaaagagc
     5221 tgttctgttg cataaaggag aaatgcatgc aatagggtcg gcaaaagatg tgatcgatca
     5281 gtaccatgca ttactatatt cagatgaaca agcttatatt cgttttgtca actcgcaaaa
     5341 agagagagaa tctcaaagtt ctttagaaga tcaaacagca gaaacattta aaacagaaac
     5401 cactgacata gtttatggtg atagtgatag tgatagtgat ggttcgagta aaaagcagag
     5461 tattactcga ggattgagtg cgcaaattgt taaatgggga atatttgatt caaatgggaa
     5521 acagtgcgag ctttttaata caggtgaatg catcactgtc actatgcaaa ttgaatcttt
     5581 atcaaacatt aacgagattc aggctggact tttactaagg acggttgagg gggtaagcgt
     5641 ttatggaact agtacccgct atcacgacag taatttaatt agcgtcaaat gtggtgatgt
     5701 ttggaattgc tcatttaata taaaattaga tttatgtcct ggctcatatt ttatcactct
     5761 tgcaatagcg gaggtattgg aacaatctgg catggcatac ttggatcgta aaactgacgt
     5821 tatggtcata aaagttaatc aacgaactct taaagctagc ggcattgctt cattagaaac
     5881 tcaagttgag gtattaaaac agaaaggata agtatgaata tactagttgt tactgaagtc
     5941 tttcttattg gtggacttga aactaatatt gctggagaaa ttcgctccct tattaaacaa
     6001 ggtcattcag tttacttggc tgttggtaaa gagttcgata caagtttaat tccttcagaa
     6061 gttactggca ttatctcaaa cttagacttg aaccataata tagggtcgga agagttttca
     6121 aacactataa atagcttacg caagtatatt agacataaca atattgaact gctgcatgct
     6181 caacctttct attctataat aatctctgct attgttgccg agttagagaa taaggcatta
     6241 gtattgactt tacatggtcc tgtgtcgttt gctaattatt tgagtccaat ttttaaattt
     6301 ttactaacag atattattct tccaaatgtc aacattgcat atgtagtttc tgctgagatg
     6361 aaagaattaa gtttggcctt ttcatcagaa agtaacgtgc agatattacc gaatggtatt
     6421 gaattaacgc agcatgatga acgttcaatc aaggatgaac gctgggtttg cgttagtcgt
     6481 ttggatgaac aaaagattgt cggcgttctt gaatttataa agtatgcaaa agctattggg
     6541 ttacctggtg ttgtcatcat gggagatgga cacgctaaag gaacattaga gcaagagata
     6601 tctgcgcata atctatcaaa ttatgttgag tttgccggat acgaatcaga tgtcagaaca
     6661 gctattactt catattctgg agtggctgga atggggagag ttgttcttga aggtattgca
     6721 gcgaaacgac gtgttatttt agttgggtat gatggtgtta aagccgtatt gaatccggaa
     6781 acttttgaaa aagctgcatg gtgtaatttc tcagggagaa atttcgatac tattactact
     6841 gaacagttaa aatgtgaatt atcatctatt tctgataaag atttagactc ttgttatgac
     6901 attatcaatg atggttataa tgtagataat atttggttgc atttctcatc ttctgtagcc
     6961 tgtattttaa acaatccttt ttcaagtacc gattcatttt tgagaaaaat ttacagtctt
     7021 attacaacag gagatgttta ttttcccaga tatgagaatt tatatttttc agatgcaatt
     7081 tatgcactga tatctcaaat catatttaat attaataatg aaagtgttcg gcaacatttt
     7141 tgtctcttag agcagaagag aaattgtgaa caattaaaac aaaataatat tagtatagct
     7201 gaaattcaaa gtattattga gttaagccaa aaaaatatct ctattaatgc tgataggtta
     7261 tatggtgatt ttgaaaagcg gatcaataaa cttattacgt ataatgatga gttaattgga
     7321 gttattgata aagcaaatgc tcagatatct aatactaaaa ctcttgtttt aggattagag
     7381 gaaaaaatac tactccttga gcgtaagata tcagaaatgg atcggaagaa tgatgaacat
     7441 atggaaaatt ccaaggggtt tttatatcga ctaactcatc gtgaaaaata aattattggt
     7501 atatctcaaa agatagataa ttttaaatgc taatttttag ttaacagagg taaaaatgaa
     7561 tttgcagagt atttctttct atgcgaagaa atctattaag ttgataagag ataaagggtt
     7621 taaggtattt attaaggttg cagtcgctaa agtcgatcga aggctgaacg gctataattt
     7681 gatacagcaa tttcctacca ttcactcctc tgataattat ctgaatgaca gtaatttcat
     7741 ggaaggtgag ctaatcatca tctctggcgt tccttatgat gatatcggag gtggccaacg
     7801 tgctgctcag cttgctaggt gtgctctgaa aaccggtaag aaagtagttt atttatacat
     7861 ttataaaaaa tatgattttg aaaaaaatat acacgttgaa tcagatgttt ctattcatgg
     7921 tttgcagcat atatatatag gaaatactac ccctaatatt atttctaaga tagtatccgc
     7981 caatgctaca ttagtcattg aacatcccca tccagatgcg cttccttatc tggagttttt
     8041 taataatcgt ggtttaaaga ctgtgtttga gcttattgat gattgggaaa ctagtcttgg
     8101 tggggactgg tttaatttgg aaaattacca acgttttgtt agagaatcaa ccgtagttgt
     8161 gggaactgcg aaattgctag taaaacgatt agaagattta ggcagagagg atgccatcta
     8221 tctaccaaat gctgcaaatg agtatatttt cgacaagtac aggaaatact ccaagccatt
     8281 tgatatgcct tctggatata atcgaatagc tctttatttt ggttcattat acggtgagtg
     8341 gtttggatgg gattatatta ccgcagctgc aaaggcaaat ccaaaaattg cctttgtact
     8401 gataggtgat aaaccaactg atagaaatat ttcagataat gtcttctttt taggtgccaa
     8461 ggatataact gaattaccag catacctggc tcattctgat ataaccttgt tgccttttgt
     8521 tccaggtaaa atttcagacg cagtatctcc tatcaaaata tttgaatatc ttttcttggg
     8581 taaaccggta attgctaccc aaatgccaga gattgtggat taccccgggg ttttcattgc
     8641 aaatgatcct gttgaatttg caacattatg taaaaaggtt gaagttacag ctgaattaat
     8701 cagtaaaaat gatatcttta tatcggaaaa ctcatggttt agtcgtctgg ataatatttt
     8761 gaccagtaaa acttatgaga gattccacaa gaaaactagt gctgttatct taatacacaa
     8821 taatgcaaat attattatcc gtaacttgca atcattgatc catcattgtg attatttcct
     8881 caaggaaata attgttgtgg ataatatgag tattgatggt ggtgctgagt tagttgcctc
     8941 tcaattccca aacgttaaga ttataaaaaa tgatgttaat ggctgctcat caggccgtaa
     9001 cttaggtgct aagtatgcta cgggccaata tttggcattt tttgattctg accagtggtt
     9061 tacatcattc tccagtttta ctgaagcttt aaatctctta gaaaaaaatg caaatattgg
     9121 cattattgga tggggtgctg gttggtttga tgtaaatcgt tctgatttag gtggtatgat
     9181 ttctgattat tgtcctaata gagggatgaa cgctgaagct atacataaag gttacagaag
     9241 tgatattggt tatcttggta cgtgcggggt atttatgtcg cgtactatat ttgaagcatc
     9301 taagggattt gatacttttt atgatcccac ttgttttgaa gatacagatt tatgtttcca
     9361 aattaaagct cttggctttg atgtttgtta tcgtgatcta actggtataa gacaccaacc
     9421 gcatcaaaca actaaagctg attctggcag tgattattac gaaaagttat ttaatcgtaa
     9481 tgctaattat ttcaaagaaa aatggtcttc ctatccagaa ttttatgtag attataaagg
     9541 aaactaaaat atgaaaaaga ttatctgtgt tgtgggcact agacctgaag ccataaaaat
     9601 ggcaccttta attcaagaat tgaaaaagtc agagttattt tctgtgactg ttttggccac
     9661 ggctcaacat cgtgaccttt tagatcaagc tctggagcta tttggaattg ctgctgatga
     9721 tgatcttaat gtaatgaccc caggtcagac attacaggaa ttaacagcaa ggttaatgat
     9781 tggagttgat aggattcttg aggaaagaaa accagatatg gttattgctc aaggtgatac
     9841 tacaacggtc ttcgttacgg ctttagcatg tttctataaa aaaataccat ttgctcactt
     9901 agaagcagga ttaaggacaa atgacttgta ttcacctttc ccggaggaag caaatcgtat
     9961 tttagcaggt catttggctg ctttacattt ttctcctacc agtgtgtccc gggataattt
    10021 gctaaaagaa aacattggtg aagagaaagt atttgttact ggaaataccg ttattgatgc
    10081 tttattggat acagccaaga aagactttga ttttgggatc gatattccac caaatcatcg
    10141 cttggttctt gttacatcgc atcgccgcga aaattttggt aagcctatag agaatatttg
    10201 cgccgcatta ctagaattgg taaataaata tcctgatatt tacgtgttgt tccctgttca
    10261 ccctaatcct aacgttcgca atgtgactaa ttctgtatta aaggggcata atcgtatcgc
    10321 tttggtggat ccactagatt atggtccttt tgtaagtgct atcaaaaaat catatctgat
    10381 tttaacagac tcaggcggag tgcaggaaga ggctccagcg ttaggaaaac cagttttagt
    10441 cttacgtcgt gatactgaaa gaccagaggc agtaactgag ggggttgtca aattagttgg
    10501 tacagagaca gttgatatta tacgcgaagc ttctattctc ctttcagata gtattgaata
    10561 tttcaaaatg gctaaaggcg catcacctta tggtgatgga aaagccagtt cccggatatt
    10621 aaatatcatt aaagaatact tcaaagttta acatcatgaa aatggtctat ctatccccgg
    10681 ttccatggtc aagcattact cagcgtccac atttttttgt ggatgctgct ttaagaaatg
    10741 atgtggaatc tgttctttgg atagacccat acccaactcg atttcctaac atcggagatt
    10801 ttcatccggg gcggcatgct ccggaacctg tgggcatggg gctacctaaa ggtcttaata
    10861 tattaaaacc tgattttatt ttgccatgtg aacccttgcc tatagtattc aaattgctta
    10921 actctggtgc tattaataaa attttccaga atgtaggtga tttcattgat tctgatactt
    10981 tattagttgt tggtaaaccg agtttgctcg cgttaaagtt aatagatagt tttaaatgga
    11041 aaaaaaaatg gtttgatgca atggatgatt ttcctcaatt ttatgtagga ctatcaaaat
    11101 ttaatatgat aaagaccgaa aataaaatca tatgttctgt tgatgaaatt atttgctcaa
    11161 gccatgcgtt attagagaag ttttctctac ctgataaaac aaaacttata ttgaatgctt
    11221 gctcggatac aattgagcgc tccagtgatt ctaacacgga taataaattt gtatttggct
    11281 atatcggtac aattgctcaa tggtttgatt ggacatgggt tgttgaatta tctaagctaa
    11341 atcctgattc tttgataaga attattggac caataaagac aagtgttcct actaatcttc
    11401 ccaaaaatat caagcttgag atggcaattg aacatagtaa ggtaatgagt acgattgcaa
    11461 cattcgatgt tgcaattata cctttcatgc tcaatgaaat cacaaaatgc gtggatcctg
    11521 taaaatttta tgaatattta ttggcccgaa aagatattgt ttcaacagcg ttcggagaaa
    11581 tgaattatca tcatcattca tatgtgaaaa atgatgttac tatcaattat aaattgcctt
    11641 ctggttcttt gttttttcca ttttcttcaa tgagtccagc accaagtgta cctcgttgga
    11701 acgatcgatt ccagactcta tttatctaat gaattctttt ggaatgtcat tttttttcga
    11761 ggcttcgaag tattcttgct tctattaatt gttaataata tttagtttgt ttttttaaat
    11821 tttatctaat ttgttttata taactatgat atattcatga tgcaaaattt ccctgcgtat
    11881 taggagcaca taatgtctaa gcaacaaatc ggcgtcgttg gtatggctgt gatggggcgc
    11941 aacctggcgc tcaacatcga aagccgtggt tataccgtgt ccgttttcaa ccgctcgcgt
    12001 gaaaaaacgg aagaggttat cgccgagaat ccggggaaaa aactggttcc ttactatacg
    12061 gtaaaagagt ttgttgaatc ccttgaaacg cctcgtcgta tcctgttaat ggtaaaagca
    12121 ggcgctggca ctgatgctgc tatcgactcc ctgaagccat atctggataa aggtgacatc
    12181 attattgatg gcggtaatac gttcttccag gacactatcc gtcgtaaccg tgagctctca
    12241 gctgaaggat ttaacttcat cggtaccggc gtctccggtg gcgaagaagg tgcgctgaag
    12301 ggaccatcta ttatgcctgg tggccagaaa gaagcttacg agctggttgc cccaatcctg
    12361 accaaaatcg ccgctgttgc agaagatggt gaaccgtgtg tgacgtatat cggtgccgac
    12421 ggtgctggtc actatgtgaa gatggtacac aacggtattg agtacggtga tatgcaactg
    12481 attgctgaag cctactcatt attgaaaggt ggtttgaacc taagcaacga agaactggct
    12541 agcaccttca ccgagtggaa taacggcgaa ctgagtagct acctgatcga tatcaccaaa
    12601 gatatcttca ctaaaaaaga tgaagatggt aaatatttgg tggatttcat cctggatgaa
    12661 gcggcgaaca aaggtacggg taaatggacc agccagagct ccctggatct cggtgaacca
    12721 ctgtctctga ttactgagtc tgtgtttgca cgttacatct cttcactgaa agatcagcgt
    12781 gttgctgcct ctaaagtcct gtccggtcct aaaactcagc ttgcgggtga taaggcagaa
    12841 tttattgaga aagtacgtcg tgcgctgtac ctcggtaaga tcgtgtctta tgctcagggc
    12901 ttctcacagc tgcgtgccgc atctgacgag cataattggg atctgaacta cggcgagatt
    12961 gcaaaaatct tccgtgcggg ctgcatcatc cgcgcgcagt tcctgcagaa aatcaccgat
    13021 gcctatgcta aaaaccctgg tatcgcaaac ctgctgctcg acccgtactt caagaacatt
    13081 gccgatgaat accaacaggc gctacgtgat gttgttgcgt atgctgtgca gaatggtatc
    13141 ccggtaccga cattctctgc tgctgtcgct tattacgaca gctatcgtgc agaggtgctc
    13201 cctgcaaatc tgattcaggc ccagcgtgac tacttcggtg cgcatactta taaacgtact
    13261 gataaagaag gtgtcttcca tactgaatgg atggaataa
//
