LOCUS       MH325886                5310 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter youngae strain O4_G3337 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325886
VERSION     MH325886.1
KEYWORDS    .
SOURCE      Citrobacter youngae
  ORGANISM  Citrobacter youngae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Citrobacter youngae"
                     /mol_type="genomic DNA"
                     /strain="O4_G3337"
                     /db_xref="taxon:133448"
                     /country="Poland"
     misc_feature    1..5310
                     /note="antigen biosynthesis gene cluster"
     gene            1280..2299
                     /gene="galE"
     CDS             1280..2299
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AWU66573.1"
                     /translation="MTLLVTGGTGYIGSHTVLQLIEGGYEVVVLDNLSNSSIESLRRV
                     ELLVSKNIPFVKADINDKDELKKILTSYNIEAVLHFAGLKSVGESVDKPLEYYINNVS
                     GSLALLEAMKEANVYKFIFSSSATVYGNCSPVPNLEDSPVGSASCPYGTTKVVLEEIL
                     SDYAKSNNKLKIIALRYFNPAGAHPSGLIGEDPNGIPNNLVPYISQVAIGKLEKLFVY
                     GGDYDTVDGTGVRDFIHVVDLAAGHVSALRHLDHVEGYEVFNLGTGQGVSVLQVIKAF
                     EKASNLKINYEIVVRRPGDIAASWADVSKAEKILNWKARFNIDDMMYHTWNWQKKNPN
                     GYKAG"
     gene            2537..3694
                     /gene="gt1"
     CDS             2537..3694
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66574.1"
                     /translation="MKIALIGTTAESLIQFRKELINSLCKNGHVVYAFAIDYDNSTKE
                     KVKSFGAIPVCYSFSRTGLNPFTDVVNTYKLYKTLKSLNLDLVFSYFSKPAIFGTLAA
                     KFAKIQKRYAMLEGLGFFFTNSGGHVSVRKKLLKKLLVSLYKISFKHIDSLVLLNVDD
                     KRDLLVNENIYVKDVHVLGGIGLNMSDYPYCPPPVDPVSFIFVARFLQEKGVYEFIEA
                     ARIVKHQFPQTQFCMLGSLDLHNPGSLTIERLNELKSNNIIELPGHVDNVQDWLAKAS
                     VFVLPSWREGFPRSTQEAMAMGRAVITSDVPGCRETVVDGVNGFLIEPRSPVALAEKM
                     LCFLYNPELIPQMGNASYQIAQQKFDSSIVNAKLMKILQLEIECLKTPEKE"
BASE COUNT     1520 a    999 c   1239 g   1552 t
ORIGIN
        1 atgattaatt taaaagcagt tattccggta gcgggcctcg ggatgcacat gttgcctgcc
       61 acaaaggcca tcccaaaaga gatgctaccg atcgttgata agccgatgat tcaatacatt
      121 gtcgacgaga ttgtagctgc aggtatcaaa gaaatcgttc tggtaacaca tgcgtctaag
      181 aacgcggttg agaaccactt cgacacctct tatgaacttg aatcgctgct tgaacagcgt
      241 gttaagcgcc agttactggc tgaagtgcag tccatctgcc ctcctggcgt gactatcatg
      301 aacgttcgtc aggcacaatt gcttggtctg gggcactcta ttctttgtgc ccgcccaatt
      361 atcggcgaca acccttttgt ggtggtgtta cctgacgtcg ttctggatac ggcgagtgcg
      421 gatccactgc gctacaacct ggctgcaatg gtggcccgct ttaatgagac cgggcgtagt
      481 caggtgctgg caaaacgtat ggcaggcgat ctttccgagt attccgttat tcagaccaaa
      541 gagcctttag ataacgaagg taaagtcagc cgcatcgttg aattcattga aaaacccgat
      601 cagccgcaaa cccttgattc agacctgatg gctgtagggc gctatgtgct ttctgctgat
      661 atttgggcgg aaattgagcg cacgcaaccc ggcgcatggg gacgtatcca gcttacggat
      721 gccattgctg agatggcgaa aaaacagtct gttgatgcca tgttgatgac gggcgacagt
      781 tacgactgcg gtaaaaaaat gggttatatg caggcatttg tgaagtacgg gctgcgtaac
      841 ctgaaagagg gaccgaagtt ccgtaagagc atcgagaaat tgcttggtgt ttagctgtct
      901 tggtcgatat taattaggtt tagttgtttt aaaaagcatg gaagattctg ggtgatatct
      961 tagctggttg atgcttctct tggctttttt atgagtaaca acaagtatcg attggtgttt
     1021 aaccgcatct tttttccttg tttctttcta caattaatac gacaatattg aggagatcct
     1081 gctgttcagg atctccaaaa tttaagcgtg cagtgcattg gtagctgttg agcctagggc
     1141 ggtagcgtgt ttaaaaaaca tgcaggtaat tgtgttgaag taggttcgtt tattctgaca
     1201 atagttgaca agaacacgta catatttaaa caaatagatt tgcagttaga atgaaataaa
     1261 ttcattttat ggaaatggca tgactctact cgtgactggt ggtacaggtt atattggctc
     1321 acatactgtt ttacagttaa ttgagggcgg gtatgaagtt gtggtgctcg acaatctgag
     1381 caattcatct attgaatcgc ttcgtcgcgt tgagttatta gtttcaaaaa atattccatt
     1441 cgtcaaagca gacatcaatg ataaagatga gctcaaaaaa atcctaacga gctataatat
     1501 tgaggctgtt cttcattttg cggggctgaa gtcagtaggc gagtctgttg ataaaccatt
     1561 agagtattat attaataatg tcagcggttc acttgcgtta cttgaggcga tgaaggaagc
     1621 gaacgtttac aagtttatct ttagctcgtc tgcaactgtt tatggaaatt gttcgccagt
     1681 accaaatctg gaagattctc ctgttggtag tgcatcttgt ccatatggaa cgacaaaggt
     1741 ggtgctagag gagatattat cagattatgc aaaatcaaat aataaattaa aaattattgc
     1801 gttacgatat tttaatcccg ctggtgcaca tccaagtggt ttgattggtg aggatcctaa
     1861 tggcatccca aataatcttg tgccgtatat ttcgcaggtt gcaattggta agttagaaaa
     1921 attgttcgtc tacggcggtg attatgacac agtggatggt acgggagttc gggattttat
     1981 ccatgttgta gaccttgccg ctggacacgt aagtgcatta cgccatcttg atcatgttga
     2041 aggatatgaa gtattcaatc ttggaactgg acaaggtgta tcagttttac aagtcattaa
     2101 agcatttgaa aaagcaagta acctaaaaat aaattatgag attgtagtca ggagacctgg
     2161 cgacatcgca gcttcatggg ctgatgtttc aaaagcagag aaaatattaa actggaaagc
     2221 tagatttaac attgatgata tgatgtatca tacatggaac tggcaaaaga aaaatcctaa
     2281 tggttataag gcaggataga tctaaaatca tgaaactgtg tcttattacc cccgttttaa
     2341 tttgattact attgccctcc tgagtgagtt gcgacgattg tctcgttgtt attttgataa
     2401 aaccagtctt ttaggtcatt acaataaata actattacat ataggatagt gttgtttgta
     2461 aaattctaac ttaaaataat ttcctgatag gatattatct gaaagtacat gcagaaatag
     2521 agtttagaac ttgactatga aaatagcatt aatcggtact acagcagaaa gtctaatcca
     2581 attcagaaaa gaactgatta attcattgtg taagaatggg catgtggtct atgcatttgc
     2641 aattgactat gataatagca ctaaagaaaa agtgaaatca tttggcgcta ttcctgtttg
     2701 ttattctttt agtcgtactg gtttaaatcc atttaccgat gttgttaata cttacaagtt
     2761 atataaaact ctgaaatcat taaatttgga tttagtgttt tcatattttt caaagccagc
     2821 catttttggt acgctggcag caaagtttgc aaaaatacag aaacgttatg caatgcttga
     2881 aggtcttggt ttctttttta ccaattctgg agggcatgtc tctgttcgca aaaaattact
     2941 aaagaaatta cttgtttcgt tatacaaaat tagttttaaa catattgata gtttagtgtt
     3001 attgaatgtc gatgataaaa gagatctttt agtaaatgaa aatatttatg tcaaagacgt
     3061 tcatgtatta ggcggcattg gtttaaatat gtcggattat ccgtattgtc ctccgccagt
     3121 tgatccagtc tcttttatat ttgttgccag atttttacaa gaaaaaggtg tctacgaatt
     3181 tatagaagca gcaaggatag tcaaacatca gtttccacaa acacaatttt gtatgctggg
     3241 aagtcttgat ctgcataatc ctggtagcct cacgatagaa aggctcaatg aattaaaaag
     3301 taataacatc attgagttac ctggtcatgt ggataatgtt caggattggc tcgctaaagc
     3361 gagtgttttt gttttgcctt catggcgtga gggttttcct cgcagtactc aggaagcgat
     3421 ggccatggga cgtgctgtaa taaccagcga cgtacctggg tgtcgggaga ccgtagttga
     3481 tggtgttaat ggttttttaa ttgagcctcg gtcaccagta gcattggctg aaaaaatgct
     3541 ttgcttttta tataaccctg agttgattcc ccagatggga aatgcaagtt atcaaattgc
     3601 ccaacaaaaa tttgattcga gcatagtaaa tgctaagttg atgaagatac tccagctgga
     3661 aattgaatgc ttgaaaacgc ccgagaaaga ataatatatt ccaggatggc aaaattgaat
     3721 acatatgtta actccaacaa ttccccaatt cattcctggc aagtagctct tatataagag
     3781 acatactgag gtaaatatgg ggctgtgaca gtttactgaa caatgctaca tttgagttaa
     3841 tatactttca tcaagagtgc gagaaaatta actcgagata ctcacacctg acaggagtac
     3901 ataatgtcta agcaacaaat cggcgtcgtt ggtatggctg tgatggggcg caacctggcg
     3961 ctcaacatcg aaagccgtgg ttacaccgtg tccgttttca accgctcacg tgaaaaaacg
     4021 gaagaggtta tcgccgagaa tccggggaaa aaactggttc cttactatac ggtaaaagag
     4081 tttgttgaat cccttgaaac acctcgtcgt atcctgttaa tggtgaaagc gggtgctggc
     4141 actgatgctg ctatcgactc cctgaaacct tatctggata aaggtgacat catcattgat
     4201 ggcggtaata cgttcttcca ggacactatc cgtcgtaacc gtgagctctc cgctgaaggt
     4261 tttaacttca tcggaaccgg cgtctctggt ggtgaagaag gtgcgctgaa aggcccatcc
     4321 atcatgccgg gtggccagaa agaagcttat gaactggtcg cgccaatcct gaccaaaatc
     4381 gcagcggttg ctgaagatgg tgagccgtgt gtgacgtaca ttggtgccga cggtgcaggc
     4441 cactatgtga agatggtaca caacggcatt gaatacggtg atatgcaact gattgccgaa
     4501 gcctattcat tgttgaaagg gggtctgaac ctgagcaacg aagaactggc cagcaccttt
     4561 accgagtgga ataacggtga gctgagcagc tatctgatcg acatcaccaa agatatcttc
     4621 accaaaaaag atgaagatgg taaatatctg gtggatttca ttctggatga agcggcgaac
     4681 aaaggtaccg gtaaatggac cagtcaaagc tctctggatc ttggtgaacc gctgtctctg
     4741 atcactgaat ctgtgtttgc acgttacatc tcttcgctga aagatcagcg tgtcgctgcc
     4801 tctaaagtcc tgtctggacc taaagctcag cttgcgggtg ataaggcaga atttattgag
     4861 aaagtacgtc gtgcactgta cctcggtaag atcgtttctt atgctcaggg cttctcacag
     4921 ctgcgtgccg catcggacga gcataactgg gatctgaatt acggcgagat tgcaaaaatc
     4981 ttccgtgcgg gctgtattat tcgcgcgcag ttcctgcaga aaatcaccga tgcctacgct
     5041 aaaaatccgg gtatcgcgaa cctgttgctc gacccgtact tcaagaacat tgccgatgaa
     5101 tatcaacagg cactgcgtga tgttgtagcg tatgccgtgc agaatggtat cccggtaccg
     5161 acattctctg ctgctgtcgc ttattacgac agttatcgtg cagaagtatt gcccgctaac
     5221 ctgattcagg ctcagcgtga ttacttcggt gcgcatacct acaaacgtac tgataaagaa
     5281 ggtgttttcc ataccgaatg gatggaataa
//
