LOCUS       MH325903               14435 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter freundii strain O41_G3915 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325903
VERSION     MH325903.1
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="O41_G3915"
                     /db_xref="taxon:546"
                     /country="Poland"
     misc_feature    1..14435
                     /note="antigen biosynthesis gene cluster"
     gene            2501..3586
                     /gene="rmlB"
     CDS             2501..3586
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 46-dehydratase"
                     /protein_id="AWU66747.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIKNTQDTVVNVDKLTYAGNLESLA
                     EVSENNRYYFEHADICDKAAMERIFATHQPDAVMHLAAESHVDRSITGPADFIETNII
                     GTYVLLEAARGYWNGLDADKKQAFRFHHISTDEVYGDLPHPDEVAVGTVLPLFTEKTA
                     YAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTKGVVGETYNIGGHNEKKNLDVVHTICDL
                     LDEIVPKEGSYREQITYVADRPGHDRRYAIDAAKISDELGWKPQETFDSGIRKTVQWY
                     LANQKWVKNVKSGAYASWLSKQYGENK"
     gene            3586..4455
                     /gene="rmlA"
     CDS             3586..4455
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AWU66748.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIKDILIITTPEDQSGFVRLLGDGKQFGINLSYAIQPSPDGLAQAFIIGENFIGNDSV
                     CLVLGDNIFFGQGFTPKLQMARNRDGGATVFGYQVMDPERFGVVEFDADFKALSIEEK
                     PAKPKSNWAVTGLYFYDNDVINIAKSIKPSARGELEITAVNEVYLNTNKLNVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNRWLTDDELTKIGLKLGKN
                     HYGQYLLKLVKGH"
     gene            4462..4860
                     /gene="fdtA"
     CDS             4462..4860
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AWU66749.1"
                     /translation="MKLIQLSKKGDDRGSLVVVEQGKDIPIEIKRVYYMFNTLKDVRR
                     GFHAHKKLTQIAIPIAGSCRFLLDDGINVENVLLDSPTIGLVIEPMVWHEMYDYSEDC
                     VLMVLADDFYDESDYIRSYEEFKMSASYEA"
     gene            4850..5383
                     /gene="orf4"
     CDS             4850..5383
                     /gene="orf4"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AWU66750.1"
                     /translation="MKHNKLEGRTIHFRLIEESDASFLYQLRMDETLNKYISKVDSSI
                     EMQKEWIRNYKEREKEHIEYYFIIERNDTNEKIGTIRLYGITDDNRFCWGSWILNSRK
                     TVTSAIESAYLIYEFAFERCNFISSYFQVDRLNSTVNSFHVKTGAIKVDEDVVNVNYI
                     FTKECFLEFKNKFRRVI"
     gene            5387..6493
                     /gene="fdtB"
     CDS             5387..6493
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta3 4-glucoseen reductase"
                     /protein_id="AWU66751.1"
                     /translation="MIKFLDLKKINEKYNAELIDAFTNVLNSGWYIMGKNLEQFESEF
                     AKYCNANHCIGVANGLDALILVLRAWKELGYLKDGDEVIVPSNTYIASILAISENNLV
                     PVFAEPDKATYNITLDSIKAVKTERTKVILPVHLYGKICPMNEIKEYAEKNKIFVLED
                     CAQAQGAELLSRKAGSWGDAGAFSFYPGKNLGALGDAGAITTDNDELAVTLKALRNYG
                     SHIKYKNQFKGVNSRLDELQAALLMVKLPGLDNENDRRREIAKIYQNYIVNKNIVLPT
                     HPENETEHVWHLYVIQTNHRQELVDYLDTKGIQTLVHYPIPPHQQNAYKELKNSSYPI
                     SELLHQTVLSLPISPVMTDEEVSYVIETINGWKS"
     gene            6481..7761
                     /gene="wzx"
     CDS             6481..7761
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AWU66752.1"
                     /translation="MEKLNFARVTILSGILTLLRLLCGFMISKVVAVYTGPAGMAGLG
                     QLQNLVTFINGFVSSQVSQGINRYSSEYKGDFNKAKYYWRASLQLSVIACAIIICIGG
                     VFSQSISSFLFTTKEFYWIIILALAVVPLNVINNIFLGVLNGLGDHYRFFLASGCAIV
                     SSVISMSILVYFLGMKGALIAAALNNAVAGLWLVFIVYKSPWFKKDFWIGRTTSFHLG
                     EMRNYFFMGVIGALTGPISLIIVRTILTKEYSVDYAGYWQSVSKLSEAYLAVLTTALT
                     VYYFPKTAAAKTVNEYISVLRSGCIIVVPFAIVFAASIFIGKEYILKILFSHDFIRAK
                     PLFLFQNIGDVFRISSWLFATILLAKGYFRINAILEITFSFMFPLLTFLCIKYFPFTG
                     VSIAYCITYVGYFFTTAIIYFYHIKKIKMQECKN"
     gene            7763..8752
                     /gene="gt1"
     CDS             7763..8752
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66753.1"
                     /translation="MQSKNKYSCSVITPVYNEEKNINKFLDVLGKVNSSSIQFIIIDD
                     GSSDKTSSIIADYLQTHNAKGNIKFISKDNGGAADARLLGIKNADSVFIAFWDCDDVI
                     EPGTIDRALEKFAEDSDIDFVLFDYYAINNGKKKRFDYSIKNWPVTGLIAFSNTIDAW
                     GIHGFGVYRKETILSGYYIAQKFTKDKENNINDDELIARAAILSARKVTMSEGRYLYS
                     DNILSTTRGINKNLYKMSSTAINLLHLIEQDSRTLDFIPAAHLYMLRVSTNLFLKKYR
                     WRRQLNNISEWDASLKKLTHQIVFKNIYIETRKKPKLFFWSVAKFLVLKSVCG"
     gene            8757..9626
                     /gene="gt2"
     CDS             8757..9626
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66754.1"
                     /translation="MNGLELVSIVIVTYNSEKFIEQTLDSCLKQTYRNIEIIISDDNS
                     EDNTLQICTEWKEKNNCEFELKIVTSNMRAGIPANCNRGAKASAGSWLKFLGADDILV
                     SDAIEKLMLSRKEHTGIIFSRFETFGDKIQFADIYPYSFTWNIIKERSCSVDDFSHWR
                     FLLGFSNVAPGVMISRETFYQNGTYDERYYLLEDLPLWYKVLSSTASVSFCESITVKY
                     RIHAHQATAAGISKILKSDLLLFNRSVRKKFAIPYYHNLLQILANSNKKYTFLKYIDI
                     FQWYISIYNRMFR"
     gene            9626..10783
                     /gene="wzy"
     CDS             9626..10783
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AWU66755.1"
                     /translation="MFVIMQCLPLLFFSLLWFFFGRTVRNTILFLCILTLSIAYTSFY
                     LNGEDWANYYVNFYTHNGSSWFEPGFWLFYSFLRLVAFGNFGIATLLFYIVTFSSLIL
                     VANKNKNCNVPAFLFFILLFFGNTLILEQLRQFIAVIIVFCALQSKMQGKSLKAYIYV
                     AISIICHASAVIALLSFVLADVKSKKTFILSTLLLSIGLSFVFVNDTVISLGVQLIPA
                     VFTKIQVYKEFAVMNFHVGPSIILAMTLLGYYLLSSYKLSYSYASEATFLQRQIFFGG
                     AVFFIGAYIPFMSRLTSYYIVFLFYDVACNHAPRYFKLYKKDVALLTFLFLFISSNFA
                     SYYKNDIAPVKFSQLTLNFVPFVSGNIDYNKRIFDIYYKNAEILSGSGKGK"
     gene            10786..11874
                     /gene="gt3"
     CDS             10786..11874
                     /gene="gt3"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66756.1"
                     /translation="MKIAMILPSLAKSGPGIQVESLCKEFLKKKYVVEVFYIRETDNV
                     SLEFYGIKKTKLHFNKGVVDSFRTFDIIHSNGFYPDLIGLLIGNYLSSTVHFTTMHNF
                     IKEDIASRYSKAKAKLYSSLWLAVIKKIKYKIVFTDIAKKYYQPMIGNTIYIAGSGID
                     IPTVEGKINDKSEITDEFTKAICHFKNRNYKIIGTTSILTSVKKIDTIINALVELKDY
                     AAIFVGGGSEENKLIKLATELNVIDRCLFIGFKENPCVYLNYFDIYAMPSSSESFGLS
                     LFEAIISRLPIICCDLPIYEELLNAHSLHKFDGTTSDFIRVISSIDDHDPDATFEAYN
                     EVKEKYSMSKIALNYYNCYQDALEHERI"
     gene            11861..12673
                     /gene="gt4"
     CDS             11861..12673
                     /gene="gt4"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66757.1"
                     /translation="MSEFSVLMSLYIKENPTFLDDCLESLYAQTVQADEVVIVLDGPI
                     NQSLKSVLDKWHLKLPIKLVPLPENVGLGEALNCGLDKCSFPLVARMDTDDICVAQRF
                     ELQLNEFSKNKELTLLGGAIAEFDGDVSNIIGKRTVASDFNQIKHLAKFRNPFNHMTV
                     MFKKDAVLNAGGYVHHHVMEDYNLWLRMLSRSNIVGNVPDILVNARAGMGMLSRRRGY
                     TYIKSELQLAKLKYRLGIQKLPGAIFIFILRAAPRVMPVFIFKKIYDLLRSK"
BASE COUNT     4397 a   2433 c   3113 g   4492 t
ORIGIN
        1 atgaacgata aagttttgct catcggtgcc tccggattcg tggggaccag actcctggag
       61 acggctgttg cggattttga tatcaagaat ctggataaac agcagagtca tttctatccg
      121 caaatcacgc aaatcggcga tgttcgcgac caacaagttc tggatcaggc gctttcaggc
      181 tatgatacgg tcgttttgct ggcggcagaa catcgtgatg atgtcagtcc tacttcactc
      241 tactatgatg tgaatgttca ggggacgcgc aatgtattgg ccgctatgga gaaaaatgat
      301 gttaagaaca tcattttcac cagttctgtt gcggtctatg gcctgaacaa aaccaaccct
      361 aatgaaaatc atcctcacga tcccttcaac cactatggta aaagcaagtg gcaagcggaa
      421 gaggttctgc gtgaatggca tgctaaagct ccagatgaac gttcgctgac catcatccgc
      481 ccgacggtta tctttggcga acgcaaccgc ggtaacgttt ataatctgct gaaacagatt
      541 gctggcggta agtttatgat ggttggggcg ggtacgaatt ataaatcaat ggcttatgtg
      601 ggtaatattg ttgagtttat caagtataaa ctgaagaatg tgactgctgg ttatgaggtt
      661 tataattacg ttgataaacc tgatctcaat atgaatcaac tggttaccga agttgaacat
      721 agcttaagta aaaaaatccc ctcagcacac ttaccttacg ctttaggcat gttaggtggc
      781 tattgctttg atattttggg taaagtgacc ggcaaaaaat atgccgtcag ctctgtgcgc
      841 gtgaagaagt tctgtgccac cacacagttt gatgcaacga aagtccactc atccggtttt
      901 gttgctcctt acacattatc tcagggactg gatcgcactt taaaatacga atttttgcac
      961 gataagaaag atgacattac gtttgtctct gagtgattgt tgtttgtaga gtgtctgggg
     1021 cttagttaac ttcgattttt cgggaatgct ataaaactga cgagatgagt ttgtttccga
     1081 ggaatccgag ttcacctcgg cgctggtgaa gtgtagagtt tgcattcggt ttacctcaac
     1141 ctggttatat cgtgttgagg atggagccag caatactggc taacgtacgg ctaaagtcta
     1201 tactttatca gccgtttgga ggtaggagag agaatgacta atttgaaagc agttatcccg
     1261 gtagcgggcc tggggatgca catgttgcca gccacgaagg caatccctaa agagatgctg
     1321 ccgatcgttg acaagccgat gattcaatac attgtcgacg aaattgtagc tgcaggtatc
     1381 aaagaaatcg ttctggtaac tcatgcgtct aagaacgcgg ttgagaacca cttcgacacc
     1441 tcttacgaac tcgaatcgtt gcttgagcag cgtgttaagc gccagttatt agccgaggtg
     1501 cagtctatct gtcctccggg cgtgactatc atgaacgttc gtcaggctca actgctgggg
     1561 ctgggtcatt caattctttg tgctcgccct atcattggtg ataacccgtt tgtcgtggta
     1621 ttacctgacg ttgttctgga taccgcaagt gcggatccgc tgcgctacaa cctcgctgct
     1681 atggttgctc gttttaatga aaccgggcgc agtcaggtgc tggctaagcg tatggaaggc
     1741 gatctttctg agtactctgt tattcagacc aaagagcctt tagacaacga aggcaaagtc
     1801 agccgcatcg ttgagttcat cgaaaaacca gaccaaccgc aaacgttaga ttccgatctg
     1861 atggctgtag gccgctacgt gctgtctgct gatatctggg cagagctgga aaaaactgct
     1921 ccaggtgcct ggggacgtat tcagctgact gatgccattg cagagttggc taaaaaacag
     1981 tctgttgatg ccatgctgat gacgggtgac agctacgact gcggtaaaaa gatgggctac
     2041 atgcaggcat tcgttaagta tgggctgcgt aacctgaaag aaggtccgaa gttccgtaag
     2101 agcatcgaga agctactgag cgagtagaga tttacaggtc tttgtgacgg taagccagaa
     2161 aaaatagcgg cagctaacat cccagcttct atgctttaag caatggaatg ttactgtcgc
     2221 ttttttatga aaaatgacca ataacaacaa gttaacctac caagttaaat catggctgtg
     2281 tcagattttt tcttgtttct ggtagcattt ggtaagacaa ttagcgtttg aatttaacag
     2341 agctttgcga tagctggcgg ggctgcttac atcttttgaa tttaggtagt gctctggtag
     2401 ctggaaggcc aggggcggta gcgtgcataa tgcccagatg cgtgcattat gtgccagcat
     2461 attaaatagt attcgtataa acgattttcg gaattaaaac gtgaaaatac ttgttactgg
     2521 tggggcgggt ttcattggtt ctgcagttgt tcgccatatt attaaaaata ctcaggatac
     2581 cgttgttaat gtcgataaac ttacctacgc cggtaacctg gagtcacttg ccgaagtcag
     2641 cgaaaataat cgctattatt ttgaacatgc ggatatctgt gataaagcgg caatggagcg
     2701 tatttttgcc actcatcaac cggatgcggt gatgcatctg gcagctgaga gtcatgttga
     2761 tcgctctatt acagggcctg cggattttat cgaaacgaat atcatcggta cttatgtatt
     2821 actggaagct gctcgtggct actggaacgg gctagatgca gataaaaaac aggcatttcg
     2881 tttccatcat atctccacag atgaagtata cggcgacctg ccacatccag atgaagtggc
     2941 tgtcggtact gtgttgcctc tgttcaccga aaagacggct tatgcgccga gtagcccata
     3001 ctctgcttca aaggcctcaa gcgatcatct ggttcgcgcg tggctgcgca cctatggctt
     3061 cccaacaatt gtcaccaact gttccaataa ctatgggccg tatcacttcc ctgaaaaact
     3121 aatcccgctg gtgattctga atgcacttga gggcaaagca ttaccaattt atggcaaagg
     3181 cgatcaaatt cgcgactggc tctatgttga agatcatgct cgagcgttat acaccgttgt
     3241 gactaagggc gttgtaggtg aaacctacaa tattggcggg cataacgaga agaaaaatct
     3301 ggacgtggtg catactattt gcgatctact agatgagatt gtgcctaaag aggggtctta
     3361 ccgcgagcaa atcacgtatg tagcggatag gcctgggcac gatcgtcgtt atgcgattga
     3421 tgcggcaaaa atcagcgatg aactgggctg gaagccgcag gaaacctttg atagtgggat
     3481 ccgtaagact gtacagtggt atttggcgaa tcagaaatgg gtaaaaaacg tgaaaagcgg
     3541 agcttatgcc tcttggttat ccaaacaata tggagagaat aaataatgaa agggattatt
     3601 ctggctgggg ggtcgggaac aaggctttat cctataacga tgggaatatc taaacaatta
     3661 cttcctgttt acgataagcc tatgatttat tacccgctgt ctgtcttaat gttagcaggt
     3721 attaaggata tattaattat tacaactcca gaagatcaat ctggattcgt tagattacta
     3781 ggtgatggaa aacagtttgg tataaatttg agttatgcta ttcaaccaag tccggatggt
     3841 ttggcacaag catttattat cggtgagaac ttcattggta atgattcggt ctgtttagtt
     3901 ttgggcgata atatattctt cggacaaggt tttacaccaa aattacagat ggcaagaaac
     3961 cgggatggcg gtgcaactgt ttttggatac caggttatgg atccagagcg gtttggtgtt
     4021 gttgagtttg atgctgattt taaagcactg agtattgaag aaaaacctgc aaaacctaaa
     4081 tcaaactggg cagttacagg tctctatttc tatgataacg acgtaataaa tattgctaag
     4141 agtattaaac cgtcggccag gggcgaatta gaaataacgg cagtgaatga agtctacctt
     4201 aacactaata aattgaatgt tgagttgtta gggagggggt ttgcatggct ggatacagga
     4261 actcacgata gtctgattga agctggcagc tttgttgaaa cggttcagaa acggcaggga
     4321 atgatggtgg cctgccctga agaaattgct tggcggaatc gttggttaac agatgatgaa
     4381 cttacgaaga ttggactgaa actaggcaag aaccattatg ggcaatactt attaaaatta
     4441 gtgaagggcc attaatttat aatgaaatta atacagctaa gtaaaaaagg cgatgatcgt
     4501 ggttctcttg ttgttgtaga acaagggaaa gatataccaa ttgagatcaa gcgtgtttat
     4561 tatatgttta acacattaaa ggatgtcaga cggggttttc atgcgcataa aaaattgacg
     4621 cagattgcaa taccaatagc tgggagttgt cgttttttgc tagatgatgg aataaatgta
     4681 gaaaatgtct tgcttgattc cccaacaatt ggactggtaa ttgaaccaat ggtttggcat
     4741 gaaatgtatg attatagtga agattgtgtt ctgatggtgt tggctgacga tttctatgat
     4801 gagagtgact atatacgtag ttatgaagaa tttaaaatga gtgccagtta tgaagcataa
     4861 taaactcgaa gggcgcacta tccattttcg tttaattgaa gaatcagatg caagtttcct
     4921 ctatcagcta cgaatggatg aaacacttaa taaatacatc agtaaagtcg atagttctat
     4981 agaaatgcag aaagagtgga taaggaatta caaggaaaga gagaaagaac atatagaata
     5041 ttattttatt attgaaagaa atgacaccaa tgaaaaaata ggcacaataa gattatatgg
     5101 cattaccgat gataacaggt tctgctgggg gagttggata ttaaatagta gaaaaactgt
     5161 aacttccgca atagaaagtg cttatctgat atacgagttt gcatttgaaa gatgtaattt
     5221 tatatcatca tatttccagg ttgatcgatt aaattcgaca gtaaatagtt ttcatgttaa
     5281 aactggtgca ataaaagttg atgaggatgt agttaacgtc aactatatct ttactaaaga
     5341 atgtttttta gaatttaaaa ataaattcag aagagtgata taaaatatga ttaaattttt
     5401 ggatctgaaa aaaataaatg aaaaatacaa tgctgagcta atagatgcgt ttacaaatgt
     5461 actaaattca ggatggtaca ttatggggaa aaatttagaa caatttgaga gtgagtttgc
     5521 aaaatattgc aatgcaaacc attgcatcgg agtcgcaaat ggattggatg cgttaatctt
     5581 agtcttaagg gcctggaagg aactaggata tttaaaagat ggtgatgaag taattgttcc
     5641 atcaaatact tacattgcgt ctattcttgc aatatcagag aataacttag ttccagtgtt
     5701 tgcggagcct gataaagcaa cctataatat cactttagat agtataaaag cagtaaaaac
     5761 agaaagaaca aaagtgattc ttccagttca tttatatggg aaaatttgcc ctatgaatga
     5821 aatcaaggag tatgcagaaa agaataaaat ttttgtactt gaagattgcg cacaagctca
     5881 gggggcagaa ctactgtctc gcaaggctgg aagttggggg gatgcaggtg cttttagttt
     5941 ttatcccggg aaaaatttag gtgcgctcgg cgatgcaggt gcaataacta ctgataatga
     6001 tgagctcgct gtcacattaa aagctttaag aaattatggc tcgcatatta aatataaaaa
     6061 tcaatttaag ggagtaaaca gtcgattaga cgaattgcag gctgctttgc tgatggtcaa
     6121 attgcctggt ctggataatg agaacgatcg tagaagagaa atagctaaaa tatatcaaaa
     6181 ttacattgtc aataaaaata ttgtgttacc aactcaccct gaaaatgaga ctgaacatgt
     6241 ctggcatcta tatgtaattc agactaatca tcgtcaagaa ttagtggatt atttagatac
     6301 taagggtatt caaacattag ttcactatcc aattcctcct caccagcaaa atgcctataa
     6361 agaattgaaa aattcttcat atcctatttc tgagctgtta catcaaactg ttttatcatt
     6421 acctatttca cctgtaatga ctgatgagga agtttcatac gttatagaaa caattaatgg
     6481 atggaaaagt tgaattttgc cagagtgacg atactaagtg ggattttgac gctcttaaga
     6541 ctgctatgtg ggtttatgat ctctaaagta gttgctgtat atactggacc tgccggaatg
     6601 gcagggcttg ggcagttaca aaatttagtg acctttatta atggatttgt atcgtctcaa
     6661 gtatctcaag gaattaacag gtactcgtca gaatataagg gtgactttaa taaggcaaaa
     6721 tattactggc gtgcttctct tcaactttct gtaatcgcat gtgctatcat tatttgtatt
     6781 ggcggggtat tctcacaatc gatatcttca tttctattca ctaccaaaga attttattgg
     6841 ataattatcc ttgcattagc ggtagtacca cttaatgtca tcaataacat atttctaggt
     6901 gttttgaatg gtctggggga ccattataga ttctttctgg ctagtggttg tgcaattgtt
     6961 tcatccgtta ttagtatgag tattttggtt tattttctcg ggatgaaagg ggcattgata
     7021 gcagcagccc tcaacaatgc tgttgctggc ctctggttag tatttattgt atataaatcc
     7081 ccttggttta aaaaagattt ttggataggt cgcacgactt catttcatct tggcgaaatg
     7141 agaaattatt tttttatggg agtaataggg gctctaacgg gtccgatctc actaataata
     7201 gtcaggacta tcttaacaaa agaatactcc gtcgattatg caggatactg gcaatcagtt
     7261 tctaaattgt cagaagccta tttggctgtt ttaacgactg cattaacggt ctattacttt
     7321 ccgaaaacag cggctgccaa gaccgtaaat gagtatatct cagttcttag gtcaggctgt
     7381 attatcgttg ttccttttgc aattgttttt gctgcaagta tcttcatagg gaaagaatat
     7441 atattaaaga tactctttag tcatgatttt ataagagcaa aaccgctatt tttgttccag
     7501 aatattggtg atgttttcag aattagcagc tggctttttg caacaattct tttggctaaa
     7561 ggatattttc ggattaatgc catattggaa attacgttct cattcatgtt tccattgcta
     7621 acatttttgt gtattaagta ttttccgttc acaggtgtta gtatcgcata ttgtataact
     7681 tacgtggggt attttttcac tactgcgata atttattttt atcatatcaa aaaaatcaaa
     7741 atgcaagagt gtaaaaacta aaatgcaaag caaaaataaa tattcatgtt ctgtgattac
     7801 acctgtttac aatgaagaga aaaacatcaa caaatttttg gatgttctag gtaaggttaa
     7861 ttcttcatct attcaattta taattatcga tgatgggtct agtgataaaa cttcaagtat
     7921 tattgcagat taccttcaga ctcataatgc caaagggaat atcaaattta tcagcaaaga
     7981 caatggaggt gctgctgatg ccaggttatt aggcatcaaa aatgctgata gtgttttcat
     8041 tgcattttgg gattgtgacg atgttatcga acctggtact atagatagag cattagaaaa
     8101 atttgctgaa gactctgata ttgattttgt cttgtttgat tattatgcca tcaataatgg
     8161 aaagaaaaaa agatttgatt attcaataaa aaattggccg gttactggtt tgatcgcttt
     8221 ttcgaatacc attgatgctt ggggcatcca tggttttggt gtttatagga aagaaactat
     8281 tctttcaggg tattatatag ctcaaaaatt cacaaaggat aaagaaaata atatcaatga
     8341 tgatgagtta atcgccagag ctgcaatatt aagtgccaga aaagtgacta tgtcagaagg
     8401 tagatatttg tatagcgaca atatcctttc gacgacaagg ggtataaata aaaatctata
     8461 caagatgtct tcaactgcaa taaatttact tcatttaatt gaacaagatt cacggacgct
     8521 tgattttatt ccagctgcac atttatatat gttaagagta tctaccaatc tgtttctaaa
     8581 aaaatacaga tggcgacgac agctgaataa tatcagtgaa tgggatgcta gcttaaaaaa
     8641 attgactcac cagatagttt ttaaaaatat atatattgaa acacgaaaaa aaccaaaatt
     8701 atttttctgg tcagttgcga aatttttagt tctgaagagt gtttgtggat aaaaaaatga
     8761 atggtctgga attagtatcg atcgttatag tgacatataa ctcagaaaaa tttattgagc
     8821 aaacattaga tagttgctta aaacaaactt atcgtaatat tgaaattatt atatcggatg
     8881 ataattctga agataatact ctgcagatat gtactgaatg gaaagaaaag aataattgtg
     8941 aatttgagct aaaaatagtg accagtaata tgagagccgg tattcctgcg aattgtaatc
     9001 gtggtgcgaa agcctcagca gggagctggc ttaaatttct cggtgctgat gatatcttag
     9061 tttccgatgc aattgaaaaa ttaatgttat ctcgcaagga acatactggg attatcttct
     9121 ctcggtttga aacttttggt gataagatcc aatttgcgga tatttatcct tattcgttta
     9181 cttggaatat aattaaagaa agaagttgca gcgtagatga tttctcccat tggcggtttt
     9241 tgctgggttt ttctaatgtt gccccaggtg ttatgatatc acgagagaca ttttatcaaa
     9301 atggcactta tgatgaacgt tactatcttt tggaagactt gccattatgg tataaagtat
     9361 tatccagtac ggcgagtgtt tccttttgtg aatctataac ggttaagtac agaattcatg
     9421 ctcatcaggc aactgctgcg ggtattagta aaattttaaa aagtgatctt ttgctattta
     9481 atagatctgt cagaaaaaaa tttgctattc catattatca taatttattg caaatactgg
     9541 caaattctaa caaaaaatat acttttttaa agtatattga tatctttcaa tggtatatat
     9601 caatatacaa taggatgttt agataatgtt tgtcattatg caatgcctgc cactattatt
     9661 cttttcactg ttatggtttt tttttggaag aacagtaagg aatactattc tttttttgtg
     9721 tatattaacc ctgtcaatag cgtatacctc tttctatctc aatggcgaag actgggcaaa
     9781 ctattatgtt aacttttata ctcataatgg ttctagctgg ttcgaaccgg gtttttggtt
     9841 attttattca tttttgcgat tagttgcgtt cgggaatttt ggaatagcga cattgctttt
     9901 ttatattgta acgttttcaa gcttaatact tgtggctaat aaaaataaaa actgcaatgt
     9961 tcctgccttt ttgtttttta tacttttatt tttcggtaac acacttatac tggagcagct
    10021 aagacaattt atcgctgtca ttattgtttt ttgtgcattg cagtctaaaa tgcaaggtaa
    10081 aagtctgaag gcttatatat atgttgctat ctctataata tgccatgcat cagctgtaat
    10141 cgcactatta tcttttgttt tagctgatgt aaaatctaaa aaaacattca tactttctac
    10201 attgctccta agtattgggc tttcttttgt ttttgttaat gacacggtta tttcattagg
    10261 agtgcagtta atccctgccg tttttactaa gattcaagtt tataaagaat ttgcagttat
    10321 gaatttccat gttggccctt caataattct cgcaatgaca ttgctgggtt attatttact
    10381 ttcatcatat aaattatcat acagttatgc atctgaagct actttccttc aacggcagat
    10441 tttcttcggt ggggctgttt tttttattgg tgcctatata ccctttatgt ccagattaac
    10501 tagctattat attgtttttc ttttctacga cgttgcatgt aatcatgcac cgagatattt
    10561 taagctatat aagaaagatg ttgcattgtt gacttttctt tttttattta tatcatctaa
    10621 ctttgcttcg tattataaaa atgatatagc tccggtgaag tttagccaac taacattaaa
    10681 ttttgttcca tttgtttcag gaaatattga ttataataaa cgtatctttg atatttatta
    10741 taaaaatgct gaaattctat ctggttctgg aaaagggaag tgatcatgaa aatagcaatg
    10801 atcttaccta gtttggcgaa aagtggtcca ggaatacagg tcgaatcttt atgtaaagaa
    10861 tttcttaaaa aaaaatatgt tgtagaggtt ttctatatac gagagaccga taatgtttca
    10921 ttggaatttt atggtatcaa aaaaactaaa cttcatttta ataagggtgt agttgattca
    10981 tttagaactt ttgatattat acattccaat ggtttttacc ccgatctgat agggttactc
    11041 ataggaaact atttaagcag tactgttcac tttactacca tgcataattt tataaaagaa
    11101 gatattgcta gcaggtatag caaggcaaaa gcaaaacttt attctagttt atggctggcg
    11161 gtaataaaga aaataaaata taaaatagta tttacggata tagctaaaaa atattatcaa
    11221 cccatgattg gaaacactat ctatattgca ggaagcggta ttgatatacc gacagtcgaa
    11281 gggaaaatta atgataaaag cgagataaca gatgagttta ctaaagctat ttgtcatttt
    11341 aaaaatagaa attataaaat catagggacg acatcgatac ttacttcagt aaaaaaaatt
    11401 gatactatta ttaatgctct tgtggagctc aaggactatg cagccatatt cgtaggcgga
    11461 gggagtgagg aaaataaact tatcaagctt gctaccgaat taaacgtaat agacagatgc
    11521 ttgttcattg ggttcaaaga aaatccatgt gtatatttga actattttga tatttatgcc
    11581 atgccaagct cgagtgaatc atttggtctt agcttatttg aggctatcat atctcgtttg
    11641 ccaataattt gttgcgactt acctatctac gaggaacttt taaatgctca tagtttacat
    11701 aaatttgatg gtacgacttc agatttcatt agagttataa gttcaattga tgatcatgat
    11761 cctgatgcga cttttgaagc atataatgaa gttaaagaaa aatatagtat gagcaagatt
    11821 gcccttaatt attataactg ctatcaggat gccttggaac atgagcgaat ttagtgtttt
    11881 aatgtcactt tatattaaag aaaatccgac atttttagat gattgtttag aaagtttgta
    11941 tgcacaaact gtgcaagctg atgaagttgt gattgtactt gatggtccaa ttaatcagtc
    12001 gttaaaaagt gttttagata aatggcattt aaagttaccg attaaacttg tgccactacc
    12061 agaaaatgtt ggattaggag aggcacttaa ctgtggctta gataaatgtt cttttccact
    12121 agttgcaaga atggatactg atgatatttg tgttgcgcaa cgatttgaac tgcaacttaa
    12181 tgagttctca aaaaataaag agttaacttt acttggcgga gccattgctg agtttgatgg
    12241 tgatgtttct aatataattg ggaaaagaac ggtagcaagt gattttaatc aaataaagca
    12301 tcttgcgaaa ttcagaaacc catttaatca tatgacagta atgtttaaaa aagatgctgt
    12361 gttaaatgcc ggtggttatg ttcatcacca tgttatggaa gattataatt tatggttaag
    12421 gatgttatcc agaagcaaca ttgttggcaa tgtaccagat atactggtta atgcaagagc
    12481 tggcatgggg atgttatcaa gacgccgtgg ctatacttat attaagagtg agttacaatt
    12541 agctaaactc aaatatagat taggtataca aaaattacct ggagcgatct ttatatttat
    12601 cttgagagcc gcaccaagag taatgcctgt atttatattt aaaaaaatat atgatctttt
    12661 gagaagtaaa taagaaacgt ttctgaatgg atagcatgta atgaataaaa acataattaa
    12721 agtattgcct ctctttttct gcaattgttg ttttgttcat tatttggctg tgttaaaaac
    12781 tatcggtatt ttagacacta agagcatatt gacattatct gtgctatttt catttctgtt
    12841 attcaatttt ttaaattacc tgtaaacaaa aaactaaaaa gtttatggtt gaaatttaga
    12901 tattagacta gcaaatggca tttaggttta tattaatgat agcaaattga tttttaaaaa
    12961 gttacgctat catttacgta gtgatatcta tcattcataa aagaaaacac acctgacagg
    13021 agtatgtaat gtccaagcaa cagatcggcg tcgtcggtat ggcagtgatg gggcgcaacc
    13081 tggcgctcaa catcgaaagc cgtggttata ccgtctccat cttcaaccgc tcacgcgaaa
    13141 aaactgaaga agttgttgcc gagaacccgg gtaagaaact ggttccttat tacacggtga
    13201 aagagttcgt cgagtctctt gaaaccccac gtcgtatcct gttaatggtg aaagcgggtg
    13261 ctggcaccga tgctgccatt gattccctga agccgtatct ggataaaggt gacatcatca
    13321 ttgatggtgg taataccttc ttccaggata ccattcgtcg taaccgcgaa ctgtctgctg
    13381 aaggcttcaa ctttatcggt accggtgttt ccggcggtga agagggcgcc ctgaaaggcc
    13441 catctatcat gcctggcggc cagaaagaag cgtacgaact ggttgcgccg attctgacta
    13501 aaatcgccgc tgttgctgaa gatggcgaac cgtgcgtgac ctatatcggt gcggatggtg
    13561 caggtcacta tgtgaagatg gtgcacaacg ggatcgaata cggcgatatg cagctgattg
    13621 ctgaagccta ttccctgctg aaaggcggcc ttaacctgtc taacgaagag ctggcaacta
    13681 ctttcaccga gtggaatgaa ggcgagctga gcagctatct gatcgacatc accaaagata
    13741 tcttcaccaa gaaagacgaa gagggtaaat accttgttga tgtgatcctg gacgaagcgg
    13801 cgaacaaagg caccggtaaa tggaccagcc agagctctct ggatctgggc gaaccactgt
    13861 cgctgatcac cgaatccgta tttgctcgct acatctcttc tctgaaagac cagcgcgttg
    13921 cggcatctaa agtgctgtct ggcccgcagg ctaaactggc tggtgataaa gcagagtttg
    13981 ttgagaaggt ccgtcgcgcg ctatatttgg gcaaaatcgt ctcttatgct cagggcttct
    14041 ctcaactgcg tgccgcatct gacgaataca actgggatct gaactacggc gaaatcgcga
    14101 agatcttccg cgcgggctgc atcattcgtg cgcagttcct gcagaaaatc accgacgcct
    14161 atgctgaaaa caaaggcatc gcgaacctgc tgctggcccc atacttcaaa aatatcgctg
    14221 acgaatatca gcaagcgctg cgtgatgtag tggcttatgc tgtgcagaac ggtattccgg
    14281 taccgacctt ctctgcagcg gtagcctact acgacagcta ccgctctgcg gtactgccgg
    14341 ctaacctgat ccaggcccag cgtgactact tcggtgcgca cacgtataaa cgtactgata
    14401 aagaaggtgt gttccacacc gaatggctgg attaa
//
