LOCUS       MH325891               15225 bp    DNA     linear   BCT 20-JUN-2018
DEFINITION  Citrobacter gillenii strain O9_G3533 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH325891
VERSION     MH325891.1
KEYWORDS    .
SOURCE      Citrobacter gillenii
  ORGANISM  Citrobacter gillenii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 15225)
  AUTHORS   Qian,C., Liu,B. and Guo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. Version 2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15225
                     /organism="Citrobacter gillenii"
                     /mol_type="genomic DNA"
                     /strain="O9_G3533"
                     /db_xref="taxon:67828"
                     /country="Poland"
     misc_feature    1..15225
                     /note="antigen biosynthesis gene cluster"
     gene            1313..2728
                     /gene="manC"
     CDS             1313..2728
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AWU66621.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLYGQRSMLQETVVRL
                     DGINAREPAVICNDEHRFLVAEQLRQINKLSHNIILEPVGRNTAPAIALAALSAIKNG
                     DDPILLVLAADHIINNKPAFHAAVKIAYEHAQKDSLVTFGIVPTGPETGYGYIHRGQK
                     IAAEENSPFKVNRFVEKPNREKAEEYISSGEYYWNSGMFMFRAKKYLAELEKYRPDIL
                     EACKLAILNAKDGDDFINVDKELFVACPDESVDYAVMEKTSDAVVVSLDADWSDVGSW
                     SALWEVSDKDHQGNVITGDTYLHNANNCYINTDEKLVAAIGVENLVIISTKDAVLVVN
                     KDQVQDVKKVVEHLKAQNRSEYKRHREIYRPWGRCDVIVQTERFNVNRITVKPGAAFS
                     MQMHHHRTEHWVILSGTGKVTIQDKVFLLTENQSTFIPIGSQHRLENPGKIPLELLEI
                     QSGSYLGDDDIIRIIDQYGRC"
     gene            2743..4128
                     /gene="manB"
     CDS             2743..4128
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AWU66622.1"
                     /translation="MTSLSCFKAYDIRGKLGTELNEDIAYRIGRAYGEFLKPKTIVVG
                     GDVRHTSEALKLALAKGLMDAGTDILDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVRENAKPISGDTGLRDVQRLAEENNFAAVDESKRGSYKQISVLDAYIDHLM
                     GYINLANFTRPLKLVINSGNGAAGHVVDALEKRFQEAGAAVSFVKIHNHPDGNFPNGI
                     PNPLLPECREDTANAVRKHNADMGVAFDGDFDRCFLFDEKAQFIEGYYIVGLLAEAFL
                     QKEKGAKIIHDPRLTWNTVDIVTRAGGTPVVSKTGHAFIKERMRKEDAIYGGEMSAHH
                     YFRDFSYCDSGMIPWLLVAELLATTDQTLSQKCTNMINNFPCSGEINYVVKDAKDLLE
                     KLDQEYGSICSKKDYLDGLSFEFEDYRFNVRISNTEPLLRLNVETKGNHDLLKSKLDS
                     IDSLIVSYNAK"
     gene            4142..5266
                     /gene="gmd"
     CDS             4142..5266
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 46-dehydratase"
                     /protein_id="AWU66623.1"
                     /translation="MSKKKVALITGVTGQDGSYLVEFLLEKGYEVHGIKRRASQFNTQ
                     RVDHIYEDPHESNVNFKLHYGDLTDASNLTRIISEVQPDEVYNLGAQSHVAVSFESPE
                     YTADVDAIGTLRLLEAIRFLGLAKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYA
                     VAKMYAYWITVNYREAYGMYACNGILFNHESPRRGETFVTRKITRGLANIAQGLSKKL
                     YMGNLDSLRDWGHARDYVKMQWLMLQQEQPEDFVIATGVQHSVREFITMAANELGIKL
                     RFEGTGVEEKGIIESIEGNKAPALKVGDVIVEVDPQYFRPAEVETLLGDPTNARVKLG
                     WTPETTLNEMVKEMVEHDFNNAQQHALLKQHGFDVNVSIE"
     gene            5282..6373
                     /gene="perA"
     CDS             5282..6373
                     /gene="perA"
                     /codon_start=1
                     /transl_table=11
                     /product="perosamine synthetase"
                     /protein_id="AWU66624.1"
                     /translation="MNMIPVYQPSLNGREKEYVMRCLDTTWISSRGEFITEFEKAFAK
                     YTGAEYATTVANGTVALHLALEALGIKSGDEVIVPTLTYIASVNTIVQTGASVVYAES
                     VEDTWNVDISDIEKRITDKTKAIMVVHLYGLSCDMDAIVKLCQERGIYLIEDCAEAFG
                     SRYKGQHVGTFGDVATFSFFGNKTITTGEGGMVLCKDKDVFDKACHLKSQGVSKEREY
                     WHDVIAYNYRMTNICAAIGLAQLEQADDILEKKRAVAASYKANLKGLPLSFQEEPEGY
                     YHSYWMCSILLDNASDRQGLRAFLKEKGVETRPLFPPANTMPHVFSDQHFPVASTLSE
                     RGINLPSFPALTTQQITYICDLIRSYFDN"
     gene            6420..7202
                     /gene="wzm"
     CDS             6420..7202
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="AWU66625.1"
                     /translation="MNSYLKGVWESRYFWIHLSLSDLRSRWRRSFFGVLWSILQPLGI
                     TILISIVFGKMFNSPITTYAPYILSGILIWDYLITCVTGGSLSFVQADAYIKQCNHPL
                     AIYTLRTVCTATFILGLASVSLLGWVLVILPHNFGLCWIAALSIFPLLILLAWPLSTI
                     LAYIGVRFRDVPHALALVMQGLWFISPVYFETKIFRSGGLHVLIDYNPVYHLLQIVRA
                     PLLQGEWPTLVNYQYVLGTALVFMVIAVLMGRKMERKVIFYL"
     gene            7199..7945
                     /gene="wzt"
     CDS             7199..7945
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="AWU66626.1"
                     /translation="MKNSIKLQDVNLHYSSVAYQERSLKSLMANIVNKKKQHKVSDIH
                     ALKDITLEINEGERVGLLGHNGAGKSTFLKTVAGLYPVSSGHLEVTGKVRSLFDLSLG
                     FEPDATGRENILYRGLLLGLSPKFMRSIQDEIVAFADLGEFIDYPIKTYSAGMQVRLA
                     FAISTSVGGNILLLDEVIGAGDANFMIKAKKRITALIEQAEILVLASHDFGTLKEICN
                     RGLLFHKGSLVFDGPIAGAIEKYKEVNRIS"
     gene            7942..10257
                     /gene="gt1"
     CDS             7942..10257
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66627.1"
                     /translation="MSDKKRVMWLLNHTSARKFEIPMLKACGVDEIFLPKSFPADPTF
                     RSASIDWSEDENLTIPKEDLDILNACDWYTGATPEAWKIANKWFDVVFFIAYDARLII
                     NLTKYFHGIIIYRVYGLPSDINYGKLLNFITEGKIANYLRRLGNRFIFGMAYDNLSNQ
                     EPDFLKRRAVYLPLGMRDVSVEDKWTGKNKKILFVCPDIAINPYYNKTYKEFVDNFSE
                     FEYVISGAQPIKVMDKNVLGFVDFETHNENMRDSMVMFYHSSEPYHIHYHPFEAIKNG
                     MPLIFMANGMLDRLGGKDLPGRCTNIADARKKIKRLMEGDKKFALSVKHSQEKLLDAM
                     NFESLLPHWVSAFNSINGINLNNTVSLKEQYKEKKIAVILPVGYLGGSLRGAILIANE
                     LYENSVKNNEPCKVIFYHVDDKIYQDDMFEDLNPRIAIRPFKWKTLDHAEANRSMEYA
                     GYEGWEIKNHEYYIPDDGINYGFDCDLWFIISDRLEKPLLPVKPIVMMVYDYIQRRNN
                     HIKNELNNRFIDVARSADKVLVTTQFTYNDALQYAGIKKEKLSKVPMLIPNFLFDEKH
                     SEETAILEQNNSNKSLGDYFIWPTNSNPHKHMDKIFQALRIYYDVYNGSMNCIVTGVN
                     TEKLLSVEGEHAVVAKKHYNESRLLKKKVKMQGNLSDLEYKIFLKNAGFLLHSSHGDN
                     GTFSVIEAAMFNVPSLANSYPAIQEMDKNYELNLTYFDVFDAHDTAKKLKWMEVNFKD
                     LKAILPTQEKLSHLAYNNHGLKYWEVVRNLL"
     gene            10254..11894
                     /gene="gt2"
     CDS             10254..11894
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66628.1"
                     /translation="MNKYGIYICFPPGVDLRHEGLGRYLAAFIKGAEKKENVQFTLLC
                     PSWSVDDIYKLFESEGVPLKLVDIISPKGTPVILNVYKYITALKNKKKKNKKSLLNRF
                     INRLKALPPNILSDFEKRVSRAYSVTDLIPVMLYGILLVLLVLIFTPFYLVLLGASIL
                     LRKIKSSSSLSIFRRIKRRVSGIFFRPKDDSLVLNLYDGMIQAETERMHTIIESLKDI
                     KAWYSPTAFWDSFTAINAPKMMCVPDVVLTQFPTAFSQIGGERVRYTYNKIIQAINKN
                     DFFVTYSNDVKQKTLVEHFNINPDNVYVVPHAPNRLDQWLDVIGFPDTKASSDFYAQQ
                     LMLVALSKQKNAYIQGLGSDFKFIFYASQARPNKNIITLLKAYNYLLKEKFWGHKLIL
                     TCGSDHLSSISNFIKENRLEYDVLFLHGLSIQELAALYKRADLAVNPTLSEGGCPFTF
                     TEALSVGTPVIMSRIPVAEEVLTDPALQEMTFFDPLDWKCLADKILWAVDNLDELKTV
                     QMQTYNQLIQRNWEDVVNEHIDALDAIADRTRVTYEHS"
     gene            11881..12504
                     /gene="orf9"
     CDS             11881..12504
                     /gene="orf9"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AWU66629.1"
                     /translation="MSTVNGIVICGFGGHARSVADVACSLGIANLIFVDDNAADGETF
                     YGFPVYKNFPEQLPEGWAAFPASGNNATRLSQIAMLEEWHWPVYTIISATATLGINTT
                     VGAGTFIGHHSHVGPNTTIGKGCIMNTGSITEHDCNVGDFTHISIGARIAGKTRIGSH
                     CFIGAGSVVIDGIKVTDKVTLGANGCIVKDIDESGVYVGTPARIIKN"
     gene            12514..13620
                     /gene="gt3"
     CDS             12514..13620
                     /gene="gt3"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AWU66630.1"
                     /translation="MNVLHFYKTYYPDSFGGVEHVIFHLTELNHIKDVECQILSLSRD
                     PTKPYVSGNNVNVVKAKELVSIASTPFSYDVIYKFKKLAQKADIIHYHFPFPFMDMIH
                     FLTDIDKPTVVTYHSDIIKQKNLLKVYTPLMNRFLKSVSAIVATSPNYFNTSKTLQKF
                     ASKVKVIPIGIDANAYTFDDASRVEYWKQKLPAKFLLFVGALRYYKGLDILLDALKQS
                     AATVVIVGAGPSEQRLKEKARTLGLDNIMFLGGVSEKDKCIILSLCYGVVFPSHLRTE
                     AFGVSLLEGALFSKPIISCEIGTGTTYINIDRETGLVAKPNDPQSLCEVMDELWQDEE
                     QAREFGINAKKRFENLFTAKHMNEEYYRLYRSLL"
BASE COUNT     4304 a   3171 c   3479 g   4271 t
ORIGIN
        1 ttgaaagcag taattccggt tgccggcctc ggcatgcata tgttgcctgc caccaaagcg
       61 attccgaaag agatgctgcc tatcgtcgat aagccaatga ttcaatacat tgttgacgaa
      121 atcgtggccg ccggtatcaa agagatcgtg ctcgtcactc actcttctaa aaacgcggtt
      181 gaaaaccact tcgacacgtc ttacgaactc gaagcgctgc ttgaacaacg cgttaagcgc
      241 caacttctgg ctgaagtgaa atccatctgc ccgccgggcg tgaccatcat gaacgtgcgt
      301 caggcgcaac cgctgggcct gggccactcc attctgtgtg ctcagcctgt cgtcggtaat
      361 aatccgttcg ttgtcgtgct gccagatatc attctggatg atgcctccgc cgatcctctg
      421 cgttacaacc ttgccgctat ggtggcgcgt ttccaggaaa cgggccgcag tcaggttctg
      481 gctaaacgta tggaaggcga cctttccgag tactccgtga ttaccactca ggagccgctg
      541 gatacggaag gtaaagttgc gcgcatcgtc gacttcatcg aaaaacccga tcagcctcaa
      601 acgctggcct ccgacgtcat ggcggtaggg cgttatgtgc tgtctgcgga tatctggcca
      661 gagcttgagc gtaccgagcc aggcgcatgg gaccgtatac agcttaccga tgcgattgcg
      721 gcgctgaaca aaacccatcc ggttgaagcg atgctcatga cgggcgaaag ctatgactgc
      781 ggtaagaaaa tgggctacat gaaagcattt gtgaattatg gtcttcgtaa tctgaaagaa
      841 ggtcaaaaat tccgcgaaag tattaaaaag atgctgtcgt aatcggttag acgtgattaa
      901 aaaaagaacg gctgcggcga tttttgtgac gcagccgttt gttcattaat caggcctgac
      961 aaaaaacggt acataattat caatagttaa gttaacatta atcttttgta tatctaaccg
     1021 acgctttagt ggtctctaaa tgaaatttgt cacatcgccg ccacaacctg tttcatcctc
     1081 tcgctgttgc ttaacagcag gtgccgtgcc cttcgtaaaa aattcctgac gttaattctc
     1141 cccgccgttt cccctaacgg acaggatgat ttgccagaaa gttttaccga gagtaccaac
     1201 aaacaacgaa gccgtacgcg ctctggttgc gatagccagg ggcggtagcg tacttcaaag
     1261 gactattttt cgttttactg cttacaaaat cgttaatgat aaggattgca aaatgctact
     1321 tcctgtgatt atggccggtg ggaccggtag tcgtttatgg cccatgtcac gtgagctgta
     1381 tccgaaacag ttccttcgtt tgtatggaca gcgttcaatg ctgcaggaaa cagtagtgcg
     1441 cctggatgga attaatgcca gagagccggc ggtgatctgt aatgatgaac accgttttct
     1501 ggtggcggaa cagctgaggc aaatcaataa gctctcgcat aatatcattc tggagccggt
     1561 agggcggaat accgcgcctg ccatcgccct ggctgcgttg agcgccatta aaaatggtga
     1621 tgatcccata ttactggtgc tggccgccga tcacatcatc aataataaac ccgcctttca
     1681 tgccgcggtt aaaatcgcgt atgagcatgc ccagaaagac agtttagtga cgttcggtat
     1741 tgtcccgacc ggcccggaaa caggctacgg ttatatccat cgtggacaaa aaattgccgc
     1801 agaagaaaac tctcctttca aagtcaaccg tttcgttgaa aaacccaatc gtgaaaaggc
     1861 ggaagaatat atcagttccg gcgagtatta ctggaacagc ggtatgttca tgttccgggc
     1921 taaaaaatac ttagctgaac tggaaaaata tcgccccgat attcttgaag cgtgcaaatt
     1981 ggctattctc aatgccaaag acggtgacga ctttattaat gttgataaag agctgtttgt
     2041 tgcctgcccg gacgagtctg ttgactatgc agtgatggaa aaaacctccg atgccgtagt
     2101 ggtaagcctt gacgcggact ggagcgatgt cggttcatgg tcagcgcttt gggaagtcag
     2161 cgataaggat catcagggta acgtgattac gggtgatacc tatctgcata acgcgaataa
     2221 ctgctacatc aataccgatg aaaagttagt tgccgcgatt ggtgttgaaa atctggtcat
     2281 tattagcacc aaagatgccg tcctcgtggt gaataaagac caggtacagg atgttaaaaa
     2341 agtcgttgag catttaaaag cgcaaaacag aagtgaatat aagcgtcaca gggaaattta
     2401 tcgtccgtgg ggacgatgcg acgtaattgt gcaaacggag cgattcaatg ttaatcgaat
     2461 aacagtgaag ccgggtgccg cattctcaat gcaaatgcac catcatcgca ccgagcactg
     2521 ggtaattctt tcaggaactg gcaaggtgac tattcaggat aaggttttct tattaactga
     2581 aaaccagtcg acctttattc ctattggctc acagcatcgc ctggaaaacc cagggaaaat
     2641 tcctcttgag ttattagaaa ttcagtctgg ttcctatttg ggcgatgacg atatcattag
     2701 aattattgac caatatggtc gttgctgatt ttaaggattt ttatgacatc actatcttgt
     2761 tttaaagcat atgacattcg gggcaaatta ggtactgagc ttaatgaaga tatcgcctat
     2821 cgaattggcc gggcttatgg tgaattttta aaacctaaaa ccatcgtggt aggcggcgac
     2881 gttcgtcaca ccagcgaagc cttaaaatta gcccttgcga aaggcctgat ggatgccggt
     2941 accgatatcc tggacattgg tttatccggt acggaagaaa tctatttcgc gacgttccat
     3001 ttaggcgtag acggcggtat tgaagtaacc gccagccata atccaatgga ttataacgga
     3061 atgaagctgg tgcgtgaaaa tgcgaagcca atcagtggcg acactggctt acgcgatgtt
     3121 cagcgtcttg cggaagagaa taacttcgct gcggttgatg aatcaaaacg gggttcttat
     3181 aagcaaattt cggtgctgga tgcctacatc gatcatctga tgggctacat taacctggcg
     3241 aatttcaccc gaccgttgaa attagtgatt aactccggta acggcgcggc agggcacgtt
     3301 gttgatgccc tcgaaaaacg attccaggaa gccggtgctg cggtcagttt tgttaagatc
     3361 cacaatcatc ctgacggtaa tttcccgaac gggatcccta acccgttatt gcctgaatgc
     3421 cgcgaagata cggcaaacgc cgtacgtaaa cataacgctg atatgggcgt tgcctttgac
     3481 ggtgatttcg atcgctgctt cctgtttgat gaaaaagcgc agtttatcga agggtattac
     3541 atcgttggcc tgctggcaga agcgttctta caaaaagaga aaggcgcgaa aattattcac
     3601 gaccctcgct tgacctggaa cactgtcgat atcgtgacca gagcgggcgg aacgccggtt
     3661 gtctccaaaa cgggacacgc gttcattaaa gagcggatgc gtaaagaaga tgcgatttat
     3721 ggcggcgaga tgagcgccca ccattatttc cgtgattttt cctattgcga cagcggcatg
     3781 attccgtggt tgttggttgc tgaattatta gcgaccaccg accagacgct ttcgcaaaaa
     3841 tgcaccaaca tgataaataa tttcccctgc agcggtgaaa ttaattatgt cgtcaaagac
     3901 gcaaaagatc tgctggaaaa actcgaccag gagtatgggt cgatttgttc gaaaaaagat
     3961 tacctggatg gattaagttt cgagtttgag gactatcgct ttaatgtaag gatttctaat
     4021 acagagcctt tattaagact caacgtagaa acaaaaggta atcatgattt gttgaaatca
     4081 aaacttgatt caatcgattc attaattgtt agttacaatg ctaagtgagt atgaggaaat
     4141 tatgagtaag aaaaaagtag cgcttattac tggtgtcact ggtcaagatg gttcttacct
     4201 ggtcgagttt ttgcttgaaa aaggctatga agttcatggc atcaagcgta gagcgtcaca
     4261 atttaatact cagcgtgttg atcatatcta tgaagatcct catgaaagta acgttaactt
     4321 caaattacat tatggtgatc tgaccgatgc gtctaacctg acccgcatca tctccgaagt
     4381 tcagcctgat gaagtgtata acctgggcgc gcaatcgcac gttgcggtga gtttcgaatc
     4441 acctgaatac accgctgacg tagatgccat cggtactctt cgtttactcg aagcgatccg
     4501 tttcctgggt ctcgccaaga aaacccgttt ctatcaggca tccacttctg agctgtatgg
     4561 cttagtgcag gaaatccctc agaaagaaac cacgccgttc tatccgcgca gcccgtatgc
     4621 cgtggcaaaa atgtacgcgt actggatcac cgtgaactac cgcgaagctt acggcatgta
     4681 tgcctgtaac ggtattctgt ttaaccacga atctccgcgt cgtggcgaaa ccttcgtaac
     4741 ccgtaaaatt acccgtggtt tagcgaatat cgctcagggc ctatccaaaa aactgtatat
     4801 gggtaacctg gattccttac gcgattgggg ccatgcgcgc gattacgtta aaatgcagtg
     4861 gttaatgctg cagcaggagc aaccggaaga cttcgttatc gcgacgggcg ttcagcacag
     4921 cgttcgcgaa tttattacca tggctgccaa cgagctgggt attaaattgc gcttcgaagg
     4981 caccggcgta gaagagaaag ggattattga atcaatcgaa ggcaataaag cacctgcgct
     5041 gaaagttggc gatgtgattg ttgaagttga tccgcaatat ttccgccctg ccgaagtaga
     5101 aacattactg ggcgacccga caaatgcacg tgtcaaactg ggttggacgc cggaaaccac
     5161 tctgaatgag atggtcaaag aaatggttga gcatgacttc aacaatgctc agcagcatgc
     5221 tctgttaaaa caacatggat tcgatgttaa cgtaagtatc gaataagacg agatcagtta
     5281 tatgaatatg ataccggttt atcaaccaag tcttaatggg cgcgaaaaag agtacgtcat
     5341 gcgctgcctg gacacgactt ggatttcttc gcgtggagaa tttataaccg aatttgaaaa
     5401 agcttttgcc aaatataccg gtgctgaata cgcaacaact gttgctaacg gcaccgtagc
     5461 tttgcactta gctttggaag cgcttggaat caaaagcggt gatgaagtta ttgttccgac
     5521 attaacctat atcgcttccg taaacaccat cgtgcagact ggcgcttctg tggtttacgc
     5581 tgagtcagta gaagacacct ggaacgtgga tatcagtgat attgaaaaac gtatcactga
     5641 taaaaccaaa gccatcatgg tggttcattt atatggcttg tcatgtgata tggacgccat
     5701 tgttaagctc tgccaggaac gtggtattta tctgatcgaa gattgtgccg aggcgtttgg
     5761 ttcccgttat aaagggcaac acgtcggcac ctttggcgat gtcgcgacgt tcagtttctt
     5821 cggcaacaaa accatcacca ccggcgaagg tggtatggtg ctgtgcaaag ataaagacgt
     5881 ctttgataaa gcctgccatc tgaagagcca gggcgtttcc aaagagcgtg aatattggca
     5941 cgacgttatc gcgtataact accgtatgac caatatctgc gcggcgattg gtctggcgca
     6001 gttagaacag gctgatgaca ttcttgagaa gaaaagagcg gttgccgcta gctataaagc
     6061 gaatcttaaa ggcttacctt tatcattcca ggaagaaccg gaaggttatt accatagcta
     6121 ctggatgtgc tctattctgt tagacaacgc aagcgatcga caaggactgc gggcgtttct
     6181 aaaagagaaa ggtgtcgaaa cccgaccact tttcccaccc gcaaatacaa tgccacatgt
     6241 tttcagcgac caacattttc cggtagctag caccttgagt gagcgtggga tcaacttacc
     6301 gagcttccct gctcttacta cacagcaaat tacttacatt tgtgatttga ttagaagtta
     6361 ttttgataat taatcaagcg cagcgcccac atttgtgggc gctaaattaa aggttttaca
     6421 tgaatagtta tcttaaaggt gtctgggaat cacgttattt ttggattcac ctgtcattat
     6481 cagatttacg ttctcgctgg cggcgttcgt tctttggagt attatggtca atactccagc
     6541 ctttagggat taccatatta atatcgatcg tttttggtaa aatgtttaat tcaccaatta
     6601 cgacatatgc gccgtacatc ctgtctggta ttttgatttg ggattatctg atcacatgtg
     6661 ttaccggtgg ttctttatcc tttgttcaag ccgatgctta cattaaacag tgtaatcatc
     6721 ctttagctat ttatacgctc agaacagttt gtactgcgac gtttatcctg ggtttggcta
     6781 gcgtatcgtt attaggttgg gtattagtca tcctgccgca taactttggc ctgtgctgga
     6841 tcgcagcatt gtctatcttc cctttgctta tcctcttagc ctggcctctg tctaccatac
     6901 tggcttatat tggcgtcagg ttccgcgatg tcccccatgc attagcgctt gtcatgcaag
     6961 gtttatggtt tatctcacct gtgtatttcg agaccaaaat attccgctca ggcggcctgc
     7021 atgtgttgat tgactataac cctgtatatc atctgttaca gattgtcagg gcgcctttgt
     7081 tacaaggcga atggcctaca ttagttaact atcagtatgt tcttggtaca gcacttgttt
     7141 tcatggtcat tgctgttttg atgggtagaa agatggaaag aaaggtgatt ttttatttat
     7201 gaaaaactct atcaagctgc aagatgtcaa tcttcattat tcctctgttg cctatcagga
     7261 acgttcctta aagtctttaa tggcgaatat cgttaataaa aaaaaacaac ataaagtttc
     7321 tgatattcat gcgttaaaag atatcactct ggaaattaac gagggcgaaa gggtagggtt
     7381 acttggccac aacggtgcgg gtaaaagtac ttttttgaaa accgtagccg gtctgtatcc
     7441 tgtttccagt ggtcatctgg aagtaacagg taaagtgcgt tcgttattcg atctcagtct
     7501 gggatttgag cctgatgcaa ccggtcggga aaacatcctg taccgtgggc ttcttttagg
     7561 cttgtcgccg aaattcatgc gcagcattca ggatgaaatt gttgcgtttg cggacttagg
     7621 cgagtttatc gattacccaa tcaaaacgta ttcagccggt atgcaagtgc ggttggcctt
     7681 cgccatttcc acgtcagtag gcggcaacat tttgctgctc gacgaggtta ttggcgctgg
     7741 tgatgccaac ttcatgatca aagccaaaaa gagaattact gcactgatcg aacaagcgga
     7801 gatccttgta ctggcatcgc atgattttgg gacacttaag gaaatctgca atcgcggttt
     7861 gcttttccat aaaggaagcc ttgtatttga tggccctatt gctggtgcaa ttgaaaaata
     7921 taaagaagtg aatcgtatat catgagtgat aaaaaaagag tgatgtggtt gttaaaccac
     7981 acatcggcaa ggaaatttga aatccccatg ttaaaggcat gcggagtaga tgaaatattt
     8041 ttgccgaagt cttttcctgc cgatcccaca ttcagaagcg catccattga ctggtccgaa
     8101 gatgaaaacc tgaccatacc aaaagaggat ttagatattc taaatgcttg tgattggtat
     8161 acaggcgcaa cgccagaagc ctggaaaatt gccaataaat ggtttgatgt cgtatttttc
     8221 attgcctatg acgcaaggtt gataattaat cttacaaaat actttcatgg cataattata
     8281 tatcgtgttt atggtctgcc ttcagatata aattacggga agttacttaa ttttattacc
     8341 gaaggtaaga tcgccaatta tctaagaaga ttaggtaaca gatttatctt tggcatggct
     8401 tatgataatt tatccaacca agagcccgat ttcctgaaaa gaagggcggt atatttgccg
     8461 ctcggtatga gagatgtttc tgtagaagat aaatggacag ggaaaaataa aaaaattctt
     8521 tttgtctgtc cagatattgc tataaatccc tattataata aaacgtataa agagttcgtt
     8581 gataacttct cagaattcga gtatgtcatt tctggtgccc agcctatcaa ggtaatggat
     8641 aaaaatgtac tgggttttgt agattttgaa acgcataatg aaaatatgcg tgactcaatg
     8701 gtcatgtttt atcattccag cgaaccatat catattcatt accatccttt cgaggccata
     8761 aaaaatggca tgccattaat ttttatggct aacggtatgc ttgacaggct tggaggaaag
     8821 gatttgccgg gtcgttgtac aaatattgcc gatgcacgaa agaaaattaa aagattaatg
     8881 gagggtgaca aaaagtttgc gttgtcagtt aagcactctc aggaaaaact tctcgatgca
     8941 atgaatttcg aaagcttgtt accgcattgg gttagtgctt ttaattccat caatggcatt
     9001 aatttaaata ataccgtttc gctaaaagaa caatataaag aaaaaaagat tgctgttatt
     9061 ttacccgtag gctatctggg cggtagttta agaggtgcca ttttaatcgc taatgaatta
     9121 tatgaaaata gcgttaaaaa taatgaaccg tgcaaagtaa tattttatca tgtcgatgat
     9181 aaaatttacc aggacgatat gtttgaggat ttaaatcctc gtatcgctat ccgacctttc
     9241 aaatggaaaa cgttagacca tgcggaagcc aatcgtagca tggagtatgc tggctatgag
     9301 gggtgggaaa ttaaaaacca tgagtattat atacctgatg atggtattaa ctatggtttc
     9361 gactgcgatc tatggttcat aatttctgat cgattggaaa agcctcttct cccggttaag
     9421 cctattgtta tgatggttta tgattatatt cagagaagaa ataatcatat caaaaatgaa
     9481 ctcaataacc gcttcattga tgtcgcccgt tctgctgata aagtacttgt aaccactcag
     9541 tttacttata atgatgcatt acaatatgca ggtataaaaa aagaaaaact gtccaaagtg
     9601 cctatgctta ttccaaactt cctcttcgat gaaaagcata gtgaagaaac ggctattctt
     9661 gagcaaaata attctaacaa atctttaggt gactatttta tttggccaac aaattctaat
     9721 ccacataaac atatggataa gatattccag gccttgagaa tctattatga cgtttataat
     9781 ggttcaatga actgtatcgt tactggcgtt aatactgaga aattgctttc ggttgaaggc
     9841 gagcacgctg tagttgccaa aaagcattac aatgaatcgc ggttgctcaa aaagaaagtc
     9901 aagatgcaag gtaacctttc tgatcttgaa tacaaaattt tcttaaagaa tgctggcttt
     9961 ttgttgcatt catctcatgg cgataacggt accttctctg ttatcgaagc agcgatgttt
    10021 aatgtcccat ctttagcaaa ttcttatcca gccattcaag aaatggataa aaattatgag
    10081 ctgaatctga catactttga tgtgtttgat gcgcatgata ctgctaaaaa attaaaatgg
    10141 atggaagtta acttcaaaga tctcaaagcc attttaccaa cacaagaaaa attgagccac
    10201 ctggcttaca ataatcatgg cttgaaatat tgggaagtgg tgagaaactt attatgaata
    10261 aatatggaat ttatatatgt tttcctccag gagtggattt acgtcatgaa ggcctgggaa
    10321 gatatctggc cgcctttatt aagggcgcag aaaaaaagga aaatgtacag tttacattgt
    10381 tatgccctag ctggtctgtc gatgatatat acaaattatt tgagagtgaa ggggtacctt
    10441 taaaattggt ggatataata tcccctaaag gaacgcctgt tattttaaat gtttataaat
    10501 atattacggc attaaaaaat aagaaaaaaa agaataaaaa gagtcttctt aacagattta
    10561 ttaatcgtct aaaggcgcta ccgcctaata ttttaagcga ttttgagaaa cgagtttcca
    10621 gagcttactc tgtaactgat ctcattcccg ttatgcttta tgggattttg ttagtcctgt
    10681 tagtgttaat ttttactcca ttttatcttg tgctccttgg agccagtatc ctgttaagaa
    10741 agataaaaag ctcgagtagc ctgtctatct ttaggagaat taaaagacgt gtctctggta
    10801 tctttttcag accaaaggat gattctttag tattgaatct atatgatgga atgattcagg
    10861 cagagacgga gcgtatgcat accataattg aatctctgaa agatattaag gcctggtact
    10921 ctccaactgc gttttgggat agttttactg ctataaatgc ccccaaaatg atgtgcgtac
    10981 ccgacgtggt actaacccaa ttcccgactg ctttttcaca aattggcggc gaaagggtac
    11041 gatacactta taataaaatc attcaggcaa tcaacaagaa tgattttttc gttacgtaca
    11101 gtaatgatgt gaagcaaaaa actctcgtag aacatttcaa tatcaatcct gataatgttt
    11161 atgttgtgcc gcatgcgcct aatcgacttg atcaatggct ggatgttatt ggtttcccgg
    11221 ataccaaagc gagttctgat ttttacgctc aacaattgat gctggtagcg ttatctaaac
    11281 aaaaaaacgc ttatatccag ggcctgggct ctgactttaa atttatattt tatgccagtc
    11341 aggcacgtcc taataagaat attattacgt tattaaaagc atataattat cttttaaaag
    11401 aaaaattctg ggggcataag ttaatcttaa cttgcggttc agaccatctt tcaagcattt
    11461 ctaattttat taaagagaac cgtcttgagt atgatgttct tttcctccac ggattatcta
    11521 tccaggagtt agccgcgctt tataaaagag cggatttagc cgttaaccca accttgagtg
    11581 aaggtggttg cccgttcacc tttaccgaag ccctatccgt gggcacccca gtcattatga
    11641 gtcgcattcc ggttgcggaa gaggtattaa ccgatcctgc cttacaggaa atgactttct
    11701 ttgatccgtt ggactggaaa tgtctggccg ataagatcct ttgggctgtg gataatttag
    11761 atgaattaaa aacggtgcaa atgcagactt ataaccagct tattcaacgt aactgggaag
    11821 atgtggttaa tgagcatatc gatgcgctgg acgcaattgc tgacagaaca cgggtaacct
    11881 atgagcacag ttaacggcat tgttatctgc ggcttcggag gccacgccag aagcgtggct
    11941 gacgttgcct gctctctggg catcgcgaat ctcatttttg tcgatgataa tgcagctgat
    12001 ggggaaacct tttatggttt ccctgtttat aaaaattttc ctgagcagct gccagaaggt
    12061 tgggcagcat ttcccgcctc aggaaataac gcgacgcggt tgtcccaaat cgcgatgctg
    12121 gaagaatggc actggcctgt gtatacgatt atttcagcaa ccgcgaccct gggtatcaat
    12181 actacagttg gtgcaggcac ttttatcgga caccattctc atgtcggccc taatacaaca
    12241 attggtaaag gctgcatcat gaataccggc agcatcaccg agcatgattg taatgtgggt
    12301 gatttcaccc atatttcgat tggtgcgcgt attgctggta aaacccggat tggctcacac
    12361 tgttttatcg gcgcaggctc tgtcgtgatt gatggtatta aagtcactga caaagtgact
    12421 ctgggcgcta acggttgtat cgtcaaagat atcgatgagt ctggcgtcta tgtgggaaca
    12481 cccgccagaa tcattaaaaa ttaagataat tacatgaatg tattgcattt ttataaaacg
    12541 tactatccag attctttcgg gggcgttgag catgttattt ttcatcttac cgaacttaat
    12601 catataaaag atgttgaatg tcagatttta tctttaagcc gtgaccctac aaaaccatac
    12661 gtcagcggta ataatgtcaa tgtggtaaaa gctaaagagc ttgtttctat cgcctccacg
    12721 ccattttctt atgatgtaat ttataaattt aaaaaactgg cgcaaaaagc agacattatt
    12781 cactatcact ttcctttccc ctttatggac atgatccatt ttcttacgga tattgataag
    12841 ccgacggtag tgacctatca ctcggatatt attaagcaaa aaaatttatt aaaggtatat
    12901 acgcctttga tgaacagatt tcttaaatcc gtatctgcga ttgtggccac ttcaccaaat
    12961 tattttaata ccagcaaaac gcttcaaaaa ttcgcatcca aagtgaaggt tatccctatt
    13021 gggatcgatg caaatgcata tacgtttgac gatgcatcgc gagtggaata ctggaagcaa
    13081 aagctgccgg cgaagtttct cctgtttgtc ggcgcgctac gttactataa aggccttgat
    13141 attcttctgg atgcgttaaa gcagagcgct gctaccgttg tcattgtcgg agcggggcca
    13201 agcgagcaaa gattaaaaga gaaagccaga acgttgggcc ttgataacat catgttcctc
    13261 ggcggcgttt ctgaaaaaga taaatgcatc attcttagtc tgtgttatgg cgtggttttc
    13321 ccgtcccatc tcagaacaga agctttcggc gtttcactac tcgaaggagc gcttttttcg
    13381 aaacctatta tttcctgtga gatcggtacg ggcacaacgt atattaatat cgatcgggaa
    13441 accggtttag tagctaagcc taatgatccg caaagccttt gtgaagtgat ggatgaatta
    13501 tggcaggatg aagagcaggc cagagagttt ggtattaatg ccaagaaacg atttgaaaat
    13561 ttgtttacag caaaacacat gaacgaagaa tattatcggt tatatcgttc attactttaa
    13621 ttttggctgc ttacctttta cgtataatta taagccatta gtatcattgt ctttatttgt
    13681 tttatttaaa tgaatctgga tgtttattta ccaacataaa gactatccct acaggcgaag
    13741 ccaggttaat atttggtaaa taactcgtca aaaatttcgt tactgataaa aaacacctga
    13801 caggagtaaa taatgtccaa gcaacagatc ggcgttgtcg gtatggcagt gatggggcgc
    13861 aacctggcgc tcaacatcga aagccgtggc tataccgtat ccattttcaa ccgctctcgt
    13921 gaaaaaaccg aagaagtcgt tgccgagaac ccgggcaaaa aactcgttcc ttactatacc
    13981 gttcaggagt tcgttgagtc gctcgaaacg ccgcgtcgta tcctgctgat ggttaaagcg
    14041 ggcgctggca ctgatgctgc tatcgactcc ctgaagccat atctcgataa gggtgacatt
    14101 attattgatg gcggtaatac ctattaccac gacaccattc gccgtaaccg tgagctgtct
    14161 gctgaaggct ttaacttcat cggcaccggc gtttccggtg gtgaagaagg cgcgctgaaa
    14221 ggcccgtcca tcatgcctgg cggccagaaa gaagcgtatg aactcgttgc cccgattctg
    14281 aaacaaatcg ctgcggttgc ggaagatggc gaaccttgtg tgacttatat cggtgctgat
    14341 ggcgcgggtc actatgtgaa aatggttcac aacggcattg aatacggcga tatgcagctt
    14401 atcgctgaag cctactccct gcttaagcac ggcctgaacc tttctaaccc ggaactggca
    14461 gataccttta ccgaatggaa taaaggtgaa ctgagcagct acctgatcga catcaccaaa
    14521 gatatcttca ccaaaaaaga tgaaaacggt atctacctgg tggatgtgat cctggacgaa
    14581 gccgctaaca aaggtaccgg taaatggacc agccagagct ccctggatct cggcgagccg
    14641 ctgtctctga tcaccgaatc ggtattcgca cgttacattt cttctctgaa agaacagcgc
    14701 gttgcggctt ctaaagtgct gagcggccct gaagttaagc cgttcaccgg tgataaagcg
    14761 gaattcgtcg aaaaagtgcg ccgcgcactg tatctgggca agattgtttc ttatgcacag
    14821 ggcttctctc agctgcgtgc cgcatcggaa gaaaacaact ggaacctgaa ctacggcgag
    14881 attgctaaaa tcttccgtgc tggctgcatc attcgcgccc agttcctgca gaaaatcacc
    14941 gatgcctatg cagaaaaccc gtcaatcgcg aacctgctgt tggccccgta cttcaaaaaa
    15001 gcggcggacg attaccagca agcactgcgt gatgtggttg cctacgctgt acagaatggc
    15061 atcccgaccc cgacgttctc agccgctatc gcttactacg acagctaccg tgcagccgta
    15121 ctgccggcta acctgatcca ggcgcagcgc gactactttg gcgcacacac gtacaagcgt
    15181 accgataaag aaggtgtgtt ccacaccgaa tggctgcaag attaa
//
