LOCUS       MF687362               17240 bp    DNA     linear   BCT 02-SEP-2018
DEFINITION  Klebsiella aerogenes strain 225_EAER polysaccharide gene cluster,
            complete sequence.
ACCESSION   MF687362
VERSION     MF687362.1
KEYWORDS    .
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella.
REFERENCE   1  (bases 1 to 17240)
  AUTHORS   Guo,X., Wang,M., Wang,L., Wang,Y., Chen,T., Wu,P., Chen,M., Liu,B.
            and Feng,L.
  TITLE     Establishment of a Molecular Serotyping Scheme and a Multiplexed
            Luminex-Based Array for Enterobacter aerogenes
  JOURNAL   Front Microbiol 9, 501 (2018)
   PUBMED   29616012
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17240)
  AUTHORS   Guo,X., Wang,M., Wang,L., Wang,Y., Chen,T., Wu,P., Liu,B. and
            Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2017) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
FEATURES             Location/Qualifiers
     source          1..17240
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="225_EAER"
                     /db_xref="taxon:548"
                     /country="USA"
                     /collected_by="UW clinical laboratory"
     CDS             1..630
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 superfamily protein"
                     /protein_id="AXR70525.1"
                     /translation="MNWQFVSFFGDSTVLLPSAVVLFAVLVLRKTSRTLAWQWALLFG
                     ITGTVVCISKLAFMGWGIGIREIDFTGFSGHTALSTAFWPILLWLLTSRASSSLRWGA
                     VLLGFILAGIVGYSRLMIHAHSVSEVVSGFILGACGSLTFLLLQWRTSAFRSAQISWM
                     GALSVVIIPLILLNTGVKAPTQSLLGEVATMIGPFDKPFTRADLHKHIY"
     misc_feature    1..17240
                     /note="polysaccharide gene cluster type 11"
     gene            1552..2991
                     /gene="wzi"
     CDS             1552..2991
                     /gene="wzi"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXR70526.1"
                     /translation="MIKIARIALALGLVASVSSQVYAAGLVQNDNELRNDLAWLSDRG
                     VIHLSLSTWPLSQEEITRALKKAKPSYSSEQVVLARINQRLSNLKADFRVSGYTSTDQ
                     PGTPQGFGQSQPADNSLSLAFNNSGEWWDVHLQGNVEGGERISNGSRFNANGAYGAVK
                     FWNQWLSFGQVPQWWGPGYEGSLIRGDAARPMIGFLMQRAEQAAPETWWLRWIGPWQY
                     QISASQMNQYTAVPHAKILGGRLTFTPFQSLELGASRIMQWGGEGRPESFSSFWDGFT
                     GKDNSSANDPNEPGNQLAGFDFKFKLEPTLGWPVSFYGQMIGEDESGFLPSANMYLGG
                     IEGHHGWGKDAINWYVEAHDTRTNMSRTNYSYTHHIYKDGYYQQGYPLGDAMGGDGQL
                     FAGKVELVTEDNQRWSTRLVYAKVNPENQTINKAFPNSDTLKGIQLGWSGDVYKSVRL
                     NTNLWYTNADNSDSDDVGASAGIEIPFSL"
     gene            3102..4238
                     /gene="wza"
     CDS             3102..4238
                     /gene="wza"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis export protein"
                     /protein_id="AXR70527.1"
                     /translation="MKKKIVRVSAVALAISFLTGCTIVPGQGLNSLRKNVVELPDSDY
                     DLDKLVNVYPMTPSLVEKLRPETILARPNPQLDQLLHSYEYRIGVGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSEVRHDIASRLTTYIESPQ
                     VDVSIAAFRSQKAYVTGEVVKSGQQPITNIPLTVMDAINNAGGLAPDADWRNVVLTHN
                     GKDTKISLFALMQKGDLTENHLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGM
                     TLAEALGNAEGMSQAFSDATGVFVVRQLKGDKEGKIANIYQLNAQDASAMVLGTEFQL
                     QPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETVRYIKTWP"
     gene            4240..4674
                     /gene="wzb"
     CDS             4240..4674
                     /gene="wzb"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="AXR70528.1"
                     /translation="MFDSILVICTGNICRSPIGERLLRKALPTKKIDSAGVGALIGHP
                     ADPSAIRIAESHGLSLEGHEGKQFSASLGRQYDLLLVMEQVHLEQVSRIAPEVRGKTM
                     LLGHWLSGKEIPDPYRKSDEAFESVYQLIDLSCRSWAEKLGQ"
     gene            4691..6856
                     /gene="wzc"
     CDS             4691..6856
                     /gene="wzc"
                     /note="in cps region"
                     /codon_start=1
                     /transl_table=11
                     /product="putative tyrosine-protein kinase"
                     /protein_id="AXR70529.1"
                     /translation="MTSVIDKKVTPQENNEIDFGRLFGELIDHRKLIISITTGFTVIA
                     VLYALFATPIYEANALIQVEQKQGNALIDSLSQMLPDSQPQSAPEIALLQSRMILGKT
                     VDDLNLQALVEQKHFPLLGKGVARLLGEKEGNLEINRLYLPKDAGGKVPEILVTVKDD
                     KHYTVDFDGIVLQGTVGVPLDEKGIALDIKSIDAKPGTQFEVKYYSRLKAITNLQESF
                     SALDQGKDTGMLNLTLTGEDPDFISRVLNSISQNYLAQNVARQAAQDAKSLEFLNNQL
                     PLVRGDLDSAENKLNQYRQQKDSVDLTMEAKSVLDQIVNVDNQLNELTFREAEISQLY
                     TKEHPTYKALMEKRKTLQDEKNKLNKRVSGMPSTQQEVLRLSRDVESGRAVYMQLLNR
                     QQELNIAKSSAIGNVRIIDDAVTDPKPVKPKKIIVILAGIVLGFIVSIAMVFVQVFLR
                     RGIESPEQLEELGVSVYASVPVSEWLTKHSAKNNIRNKNQSDRLLAVENPADLAVEAI
                     RSLRTSLHFAMMEAENNILMISGASPNAGKTFISTNLAAIIALTGKKVLFIDADLRKG
                     YVHKMLGHKQTKGLSDILSGQSSVESTIEKVTSGEFDYIGRGQVPPNPAELLMHSRFS
                     DLLKWASSNYDLVVIDTPPILAVTDAAIIGRYAGTTLLVARFESNTVKEIAVSVRRFE
                     QSGIDVKGAILNGVVKKASSYYGYGYSYYGYSYSDKEKS"
     gene            6958..8385
                     /gene="wbaP"
     CDS             6958..8385
                     /gene="wbaP"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase"
                     /protein_id="AXR70530.1"
                     /translation="MTRFARNVGCSVSLAVSDFLSFVLSLYLSLGILSLTMSHFDERV
                     PNEQIEGWVALHWLLGICCISWYGIRLRHYFYRKTFWFELKEILRTLVIFAVIEIAVI
                     AFAKWYFSRYVWLLTWVIAAILLPLTRMFTKWLLNRMGLWLRDTWIVGNGKNAIDAYN
                     AINSERNLGLDVIGFISCDGETRTVEHTNKTVVLDANKNWLAGIDKRTQFIVAVESNQ
                     SEIRNNWLREFMIKGYRFVSVIPTLRGVPLDSTDMSFIFSHEVMIFRVQQNLAKLSSR
                     IIKRGFDIFGSMGIILILSPLLFYIYNKVKKDGGPAIYGHERVGKDGKPFKCLKFRSM
                     VVNSKEVLDNLLASDPEARAEWDATFKLKNDPRITKIGQFLRSTSLDELPQLFNVLKG
                     EMSLVGPRPIITAELERYNEEVDYYLLSKPGMTGLWQVSGRSDVDYDTRVYLDAWYVK
                     NWSMWNDIAILFKTVGVVLKKDGAY"
     CDS             8498..9544
                     /codon_start=1
                     /transl_table=11
                     /gene="GT1"
                     /product="glycosyltransferase"
                     /protein_id="AXR70531.1"
                     /translation="MNKKEVIAVVFTAAIVGGHELMTIAHLNKFRNKGFRFACFVPSD
                     NIALKKILTDHNISYENHEVLHKKFEILHSTFNYPFRKKAKELLVSLKGKHKTIILVQ
                     GDIELGSVFVNTSKAAGVKNLVSYIPYAHSFRLMGSKLGWLKDILSKFVYKKCENYIT
                     ISKVFAEQISLKNKNAKVRVLHNFSSKLPIEYIRGKGYIYKKDENKFTILMAGRVSFR
                     QKGQDILLQALNSISTSKEIVLKVIGDGPDLEKLKTITECSRNNIRVNYYGWQPNVWE
                     YSKDVDLITLPSRFEGVPLLMLEAKQANIPIIAPARDGMIDFLPPTNLYEPGSRDSEA
                     AALAKKIQTIINNH"
     CDS             9562..10545
                     /codon_start=1
                     /transl_table=11
                     /gene="GT2"
                     /product="glycosyltransferase"
                     /protein_id="AXR70532.1"
                     /translation="MKNILIDDRWSGSGGIGTFAEEINKITNYKSAGFTGKPFSPVDC
                     IKTSLKMMKMRDGIVFFPGYIPPLFSRVPFVFTIHDLNHLERPENSSLLKKIFYKVVI
                     KRGCRKAKYIFTVSEFSRQKIIQWSGVPADKVINVGNGVSDDFRPNGQKKDFGYPYYL
                     CVSNRKSHKNEVGTIQAFARADIPKNVKLVFTGKKTDELATLIEINELTDRVVFTGFV
                     KTEELPELYRGAEGLIFVSFYEGFGLPVIEAMASGIPVITSKTTSLGEVAGDAALVVD
                     PENIQQIATAISKLHSDIELKTQLISKGSKQVGKYSWSATAELVNKYLTSS"
     gene            10607..12148
                     /gene="wzx"
     CDS             10607..12148
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AXR70533.1"
                     /translation="MSEKYATNKKIVAKNTFMLYGRMLLIMFATFYTSRLVLNALGVY
                     DFGLYSVIGSIIVLFSFIQNVSALATQRFLSVGLGRRDFSWTKRVFSTSILVHFIICG
                     AVLFLAETIGLWFLIYKLRVPVERMTEIFWVYQLSVISLLIQIMQTPFIAALIATEKM
                     DLFAKIGIFDAIQRWGMVYLFTLFEFDSKLIWYAAFLLFGYISVFFVYFVSCIKKIDI
                     CTVKVSFKENKELLKEILSFSSWSMMGSLSLVGLSQGIALLTYFFVGVVANGSVWLAE
                     QVLIAFNRVIGTLQTAFNPQLIKQHSTGNHEEVSSLLSLSCKLTGFIVLIAAVPAFVD
                     APFVINLWLKQVPEHLVVLVRIVILYVIIDSLSGPFVTVAYAVGKLRNYQLIASLIML
                     LTLVLTYALYSLGFALVVAVSSRILCAVAMLMFRVLYVEKVTGLNSKIFFLNIFPRLF
                     SVSVISFAISFYINTVLPVGFLGLLLLIGLNTIAVAILFFYLVLISSERKYLTSLIKK
                     KLGRK"
     CDS             12150..13139
                     /codon_start=1
                     /transl_table=11
                     /gene="PT"
                     /product="polysaccharide pyruvyl transferase"
                     /protein_id="AXR70534.1"
                     /translation="MQVNNTQYGVLTYESDIYVKRNWINLGDYVQSTAARQFLPCVNE
                     YIPRDHMNTYSGNKVKMIMNAWYMDIPENFPPSEDITPLYVSIHINSTIVDKIFTPAS
                     IEHFKKHAPIGCRDFYTRDLLISKGIDAYYSGCMTLTLGNKYKRDNVTDDIYFIDVMY
                     DSMSLPELMSQPLRFGKRLLNGRAFEFNHRSKVLNQFFDSELLKHAKHEKQIIPYLSA
                     DEGFKLADDFLRKLANAKLVVTSRIHTALPCLAMGTPVIFVNGGFKNKVDNCRFDGLF
                     DFFNRIDVDMNANATANFEFDGKKIGMNTIIENKTLHEQYANELADKCRSFVV"
     gene            13195..14616
                     /gene="manC"
     CDS             13195..14616
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AXR70535.1"
                     /translation="MILPVIMAGGTGSRLWPMSRELYPKQFLRLHGEQSMLQETITRL
                     NGLETKEPIVICNEQHRFLVAEQLRQIEKLSNNIILEPIGRNTAPAIALAALHAVAHD
                     DDDPLLLVLAADHVIENNAAFQAAINSAVVYANQGKLVTFGIVPTGPETGYGYIQRGN
                     EEHFDNHIAYDVKRFVEKPNLHTAQEYLDSGEYYWNSGMFMFRASRFLQELDEFRPDI
                     LSSCKKAMQTVHTDSLRDFIRVDKDAFIACPDESVDYAVMEKTQSASVVPLDAGWNDV
                     GSWSALWEVNDKDGQGNSLSGDVFVHDSSNCYINSEEQLVAAVGVNNLVVVSTKDAVL
                     VIDKSQVQDVKRVVEHLKMTSRREYRIHRESYSPWGHSDKVVNTTKYHINKVTVKPGG
                     KFSLQMHHHRAEHWIILSGTAKITLNEREFLLTENQSTFIPAGSTHMLENPGKIDLEI
                     LEIQSGSYLGEDDIVRLKDHYGI"
     gene            14633..16006
                     /gene="manB"
     CDS             14633..16006
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AXR70536.1"
                     /translation="MELLTCFKAYDIRGKLGSELTEDIAYRIGRAYGEYLKPKKMVVG
                     GDVRLTSESLKLALAKGLQDAGTDVLDIGLSGTEEIYFATSYLGVDGGVEVTASHNPI
                     DYNGMKLVRENSKPISGDTGLREIQRLAEENNFAINSSQPKGSYQSISVLDAYIDKLL
                     SYINLHNINRTLKLVVNSGNGAAGHVIDALEKRFIKHNVPVEFIKVHHEPDGTFPNGI
                     PNPLLPECRKDTTDAVIKNNADMGIAFDGDFDRCFLFDNMGNFIEGYYIVGLLADAFL
                     EKNPGSRIIHDPRLSWNTIDIVKNAGGIPVMSKTGHAFIKERMRAEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCIKDKTLNQLVSARMQAYPASGEINSSLSDPQSSIK
                     RVLERYQNEALDINYTDGISLEFADWRFNLRSSNTEPVVRLNVESRSDVALMKEKTAE
                     SLALLRA"
     CDS             16089..17240
                     /codon_start=1
                     /transl_table=11
                     /gene="GT3"
                     /product="glycosyltransferase"
                     /protein_id="AXR70537.1"
                     /translation="MRDLNVGIVADWFITYAGSEKVVAELIKSYPESHLYSVVDFLSS
                     ENKKHFQNKEITTTFIQRLPKAKKKYQTYLPLMPLAIEQLDVSSHDVILSSSHAVAKG
                     VLTGPDQLHISYVHSPIRYAWDLQHQYLREAGLDHGVKGMIAKLILHKMRMWDYRTAN
                     GVDHFIANSQFIARRIKKVYGREADVIYPPVDVNRFLLNEAKEDYYFTASRLVPYKRI
                     DLIVEAFSHMPNRKLVVVGDGPEMQKIKSKATANIEILGYQSNEVMQEHMRNAKAFVF
                     AAEEDFGITPVEAQSCGTPVIAFGKGGSLETVRPFGVDNPTGILFDKQDIDSVIDAVQ
                     KFETMQDQILPNDCRAHALKFSTERFNQEISEYVNSKYCEFVENKKIIY"
BASE COUNT     5177 a   3006 c   3806 g   5251 t
ORIGIN
        1 atgaactggc aatttgtctc gttttttggc gatagtaccg tgttgcttcc tagtgcagtg
       61 gtgctgttcg cggtgctggt gctgcgtaaa acatcccgga ctctggcctg gcaatgggcg
      121 ctgctctttg ggattacggg gactgtcgtg tgcatttcaa aactggcctt tatgggctgg
      181 ggaattggca tacgggaaat cgattttacc ggtttcagcg gacataccgc actctcaacc
      241 gccttttggc caatactctt atggctgtta acttctcgtg cttcatcatc cctgcgctgg
      301 ggggccgtgc ttctgggctt tattctggcg ggtattgtgg gctattcccg attgatgatt
      361 catgcacatt cggtatctga agtggtgagt ggatttattc tgggtgcttg tggaagtttg
      421 acttttctgc tgttgcaatg gcgtacgtcg gcttttcgtt ctgcgcagat ttcatggatg
      481 ggggcattga gcgtggtaat tatcccactc atattattaa acactggggt taaagcaccg
      541 acgcagtcct tgttaggcga ggttgcaacc atgatcgggc catttgacaa gccgtttacc
      601 agagccgatc tacataagca tatatattaa tcatactagt tcgatatgtt taagggttaa
      661 ctgctggcgt gattagtatg ataaatagcg tatccatagc tagctaaggg attttagaat
      721 cgtgaccttc ataatggtga aacataataa tgttaacagg atccttgaaa gcacggtccg
      781 gtatgttatt acggataaga tgattcttaa atgaaaagag aataacggaa atcagagtta
      841 tcgttctgga acccaaaaaa gaagtgggta atgatatcta tcatattttg tagttgacta
      901 ctgcatgggt atttttttac ttaaacatgg tatttatttt aatcgattta aaatcatata
      961 gttaccatgg atcattctta gggtggtaat aaagtggtca gctggtgaga gatgccatta
     1021 aataatctta ttaacagatg aacccttgcc ggtaaaaatg aaaaatatcg ttttatccgc
     1081 ttcgtggggg ggaatttata ctcccaatta acagaagaat ctcgtaaact cgaggcggta
     1141 aaaataattg ctgtggttaa ttcaccaaca gccgatgtaa agaaaattcc tgggtaagag
     1201 gtacagtgcg ttggtagctg ttaagccaag ggcggtagcg tatccagaca ctcacggtac
     1261 ccaatgtttt gaatactcac catacgcata gcgacgttgt cagacttctg atcatgtgca
     1321 cattattgag cccatgaaag tagccttggc aaccaatatc gcttacatta tgaatgcatt
     1381 aagttcacat ccgtttgcag caaaccgctc gtacctgagg atgttgtcca tcgaggcaat
     1441 ggatcgctgt tgtaaaaaat aggcttgtcg aactcataag ctcctgattc ttaaaaatcc
     1501 ccattgccgt acccacctgg tgggaaggga ctcgtaaatg gaacttgaac aatgataaaa
     1561 attgctcgta tcgcacttgc tttgggtctg gttgcatcag ttagctcgca ggtatatgca
     1621 gcagggttgg tgcaaaatga taatgaactt cgcaacgacc tggcgtggtt atccgatcgc
     1681 ggagtcattc atctaagtct ctctacctgg ccgctcagcc aggaagaaat tacccgagcg
     1741 ctgaaaaaag caaaaccttc atattcctct gaacaagttg tcctggcgcg catcaatcaa
     1801 cgcctttcta atttgaaggc ggattttcgc gtaagcggct atacgtcaac tgaccagcct
     1861 ggaacacctc agggattcgg ccaaagccaa ccagcagata attctctctc tcttgccttc
     1921 aataacagcg gtgaatggtg ggatgtgcat ctgcagggga atgtcgaagg cggtgagcgt
     1981 attagcaatg gatcacgttt taatgccaac ggcgcatatg gcgcagtcaa gttctggaat
     2041 caatggttat cattcggcca ggtgccccag tggtggggcc ctggttatga aggtagcctg
     2101 attcgtggcg atgcggccag accaatgatc ggttttttaa tgcaacgcgc tgaacaagct
     2161 gctcctgaga catggtggct gcgctggatt ggtccgtggc agtatcagat ttccgctagc
     2221 cagatgaacc aatatacggc cgtacctcat gctaagatcc ttggtgggcg tcttactttc
     2281 acaccttttc agtcacttga gcttggcgct tcacgtatta tgcagtgggg gggtgaaggg
     2341 cgtcctgaat cctttagcag tttttgggat ggctttactg gcaaagacaa ctcgtcagcc
     2401 aacgacccaa atgaaccggg taaccaactt gccggctttg attttaagtt taagctcgaa
     2461 cctaccctgg gctggccggt gagtttctat ggccaaatga ttggcgaaga tgagtcagga
     2521 ttcttacctt ccgcgaatat gtatcttggt ggtattgaag gtcaccacgg ctggggtaaa
     2581 gatgcgatta actggtatgt ggaagcgcat gatacccgta ccaatatgag tcgcactaat
     2641 tacagttata cccatcacat ctataaagat ggttattatc agcaaggcta tccactaggt
     2701 gatgcgatgg ggggagatgg ccagttgttt gcaggcaaag ttgagttggt gactgaagat
     2761 aatcagcgct ggagtacccg tctggtgtac gcgaaagtga acccggaaaa tcagactatc
     2821 aataaagcgt ttcctaattc agacacctta aaagggatac agttgggctg gagtggcgat
     2881 gtctataaat ctgttcgatt gaatactaat ctttggtaca ccaatgccga taacagcgat
     2941 agcgacgatg ttggtgccag tgcagggata gagattccat ttagtttata ggctggatgc
     3001 tgagaagagg gtagggatta ctgattgtga attgttaatc ggtaaaaact cagacggcaa
     3061 atcagtaatt gaaaaatttg gtttattgat gatgtgacat tatgaagaaa aaaattgtta
     3121 gggtttcagc agtggcattg gcaattagct ttttgacagg gtgtactatt gttccgggtc
     3181 aggggctgaa cagcttacgc aaaaatgtag ttgaacttcc ggatagcgat tatgacctgg
     3241 ataagttggt gaacgtttat ccaatgacgc cgagtttggt tgaaaagctt cgaccggaga
     3301 ccattcttgc tcgcccaaat ccgcaactcg accagttgct tcatagctat gaataccgaa
     3361 ttggtgttgg cgatgtactg atggtgactg tctgggatca ccctgaacta acgactcctg
     3421 caggtcagta tcgtagtgct agcgatacgg gtaactgggt aaactccgat ggaacaatct
     3481 tttatcctta tataggtaag gttcaggtcg caggtaaaac gttgagcgaa gtacgtcacg
     3541 acatagcgag tcgattaaca acctatattg aaagcccgca agtagacgtg agtattgctg
     3601 catttcgttc acaaaaagcc tatgtcaccg gagaggttgt taaatctgga caacagccga
     3661 tcacaaatat accgctaacc gtaatggatg caataaataa tgctggtgga ttagctcctg
     3721 atgcagactg gcgtaacgtt gttttaactc acaacggcaa agacacaaaa atatcgctgt
     3781 ttgcattaat gcaaaaaggt gatttgacag aaaaccattt actctacccg ggcgatatat
     3841 tgttcgtacc aagaaatgat gatctgaaag tgtttgtcat gggggaagtt ggtaaacaaa
     3901 gtaccatgaa aatggatcga agcggcatga cattagctga ggcattgggt aatgctgaag
     3961 ggatgtcaca ggcgtttagt gatgccacag gggtctttgt cgtgcggcag ttaaaaggcg
     4021 ataaagaggg taaaatcgcc aatatttacc agcttaatgc acaggatgca tcggccatgg
     4081 tgttaggtac agaattccag ctacagccgt atgacatcgt atatgtcaca acagccccat
     4141 tggtgcgatg gaatcgcgtg atatctcagc tagttccgac aattactggg gtacatgata
     4201 tgaccgaaac tgtccgatat atcaaaacgt ggccataata tgtttgactc aatattagtt
     4261 atttgtaccg gtaatatttg tcgctctccg attggagagc gattattacg taaggcatta
     4321 ccgactaaga aaatagactc agcgggtgtt ggtgccttaa tcggtcatcc tgcagatccc
     4381 tcagcgatac gaattgctga aagtcatggt ctttctttgg aaggacatga aggtaagcaa
     4441 tttagcgcaa gtcttgggcg ccaatatgat ttgctcttag taatggagca agttcattta
     4501 gaacaagtca gtcggattgc tccagaagtt agggggaaaa caatgcttct tgggcattgg
     4561 ctatcaggta aagagatacc agacccttac aggaagagcg atgaagcatt cgaatcagtt
     4621 tatcagttga ttgacttaag ttgtcgttct tgggctgaga agttagggca ataatatcgg
     4681 gaaaaaccgc atgacatcgg taattgataa aaaagtaaca ccacaagaaa ataatgagat
     4741 tgattttgga cgtttgttcg gtgaattaat tgaccatcgc aaactcatta tttctataac
     4801 cactggcttt acagttatag cagttttata tgctctgttt gccaccccaa tttatgaagc
     4861 taatgcatta atacaggttg aacaaaagca gggtaacgca ttaatagata gcctcagcca
     4921 aatgttgccg gatagtcaac ctcaatctgc gccagaaata gcattactgc agtcacgtat
     4981 gattctagga aagactgttg atgatcttaa tcttcaggct cttgttgaac agaaacattt
     5041 tccactatta ggaaaggggg ttgctagatt attaggtgag aaagaaggta atctggagat
     5101 taatcgcctt tatttgccga aagatgctgg gggaaaagta cctgaaattt tggtaacagt
     5161 aaaagatgat aaacattaca ctgtcgattt tgacggtatc gttttacagg ggaccgttgg
     5221 tgtaccactg gatgagaaag gtattgctct cgatataaaa tcgattgatg caaaacctgg
     5281 aacgcaattt gaggttaaat attattctcg actgaaagca atcacgaatc tgcaggagtc
     5341 atttagcgct ttagatcagg gcaaagatac gggaatgctt aacttaactt tgaccggtga
     5401 agaccctgat ttcatttcac gagtattaaa cagtatcagt caaaattatc tggctcaaaa
     5461 cgttgcgcgt caagccgcgc aggatgctaa aagcctggag ttccttaata atcaattacc
     5521 cttggtacgt ggagatttag attcagctga aaacaagttg aatcaatatc gacaacaaaa
     5581 agactctgtt gaccttacca tggaggcaaa atcagttctg gatcagattg ttaacgttga
     5641 taatcaattg aacgaattaa catttcgtga agcagaaatt tctcaacttt atacaaaaga
     5701 acatcctact tacaaagcac tgatggaaaa aaggaaaaca cttcaagatg aaaaaaataa
     5761 gctaaataaa cgtgtcagtg gaatgccctc gactcaacaa gaagtgttgc gccttagtag
     5821 ggatgttgaa tctggtcggg ctgtatatat gcaattactc aaccgtcagc aagagttgaa
     5881 tattgctaaa tcaagcgcaa ttggtaacgt cagaatcatt gatgatgctg taaccgatcc
     5941 gaaaccagtt aaacctaaaa aaataattgt aatattagct ggcattgtac ttggttttat
     6001 cgtgtcaata gctatggtct ttgtacaagt attcctgcgt cgtggtattg aatctccaga
     6061 acagttagag gagctgggag taagcgtgta tgcgagcgtt ccagtatctg aatggttaac
     6121 taaacattct gcgaagaaca atatacgaaa caaaaatcaa tcggatagac tgctggctgt
     6181 agaaaaccct gcagaccttg cggtagaggc aattaggagt cttcggacga gcttacattt
     6241 tgcgatgatg gaagccgaaa ataatatact gatgatttcc ggagcaagcc caaatgctgg
     6301 taagacattc ataagtacta acttggctgc gatcattgct ctgactggga agaaagttct
     6361 ctttatagat gctgacctca gaaaaggtta tgtgcataag atgctcggcc ataaacagac
     6421 aaaaggatta tcagatatac tttctggcca aagttctgta gaaagtacaa tagagaaggt
     6481 taccagtggc gaatttgact atattggacg tggtcaagtt ccaccaaatc ctgccgaact
     6541 gctaatgcat tcaagattct cagatttgct taagtgggct tcatctaatt atgacttggt
     6601 cgttatagat actcctccta ttttagcagt caccgatgct gcaattattg gtcgttatgc
     6661 agggacaaca ttactagtag caagatttga atcaaatact gtcaaagaaa ttgcagttag
     6721 tgtaagacgt tttgagcaaa gtggcattga tgttaagggt gctattttga acggagtcgt
     6781 taaaaaagca agtagctatt atggctatgg ctatagttat tacggttatt cgtattcaga
     6841 taaagaaaaa agttaaaaat ttgaaaggtt aaaacaaaac gggctctatt tatagagtcc
     6901 gtattctatt ccatagtaac tttctccatg ttaagcgtgg tataagcaga gggaatcatg
     6961 acccgttttg ctagaaatgt tgggtgcagt gtatccttag cggtatctga ttttttaagc
     7021 tttgtattat ctctatattt atctctcgga attctttcgc ttacaatgag tcattttgac
     7081 gaaagagttc ctaatgagca gattgaaggg tgggttgctt tacattggct tttaggtatt
     7141 tgttgcatta gttggtacgg aattcgtctg cggcattatt tttatcgtaa aactttttgg
     7201 tttgagttaa aagaaattct ccgtactttg gtaatatttg ctgttattga aattgctgta
     7261 atagcatttg caaaatggta tttttcacgg tatgtatggc ttttaacatg ggtaattgca
     7321 gctattttgc taccattaac caggatgttt actaaatggc tgttgaatcg aatgggcctt
     7381 tggttgcgag atacatggat agtgggaaat ggtaaaaacg ctatcgatgc ctacaacgca
     7441 ataaatagcg aacgaaatct cggtttagac gtcattggtt ttatttcttg cgatggtgaa
     7501 actagaactg ttgaacatac taataaaaca gtagtgctag atgctaataa aaattggtta
     7561 gctggcattg ataagcgtac gcagttcatt gttgcagtcg aatcaaatca aagtgaaatc
     7621 cgaaataact ggcttcgcga gtttatgatc aaagggtatc gttttgtctc ggtaattcca
     7681 accctacggg gcgtaccatt agatagtacg gatatgtcat tcatatttag ccatgaagtt
     7741 atgatattta gagtacagca gaatctggct aaattatctt cgcgaattat taaacgaggt
     7801 tttgatatat ttggctcgat gggtattatt ctaattttat ctccactttt attttatata
     7861 tataataaag ttaaaaagga tggcggtcct gctatttatg gtcatgagcg cgttgggaaa
     7921 gatggtaagc ctttcaaatg cctgaagttt agatcaatgg tagttaactc aaaagaggtc
     7981 ctggataact tactggcgag cgatccagaa gctcgagccg aatgggatgc aacatttaaa
     8041 ttgaaaaatg atcccagaat caccaaaatt ggccaatttt tacgtagtac aagtcttgat
     8101 gagctccctc aattgtttaa tgtattaaaa ggggagatga gtctggttgg cccacgccct
     8161 attattaccg cagagttaga aagatataat gaagaagtcg attattattt acttagtaaa
     8221 cctggaatga caggactatg gcaagtgagt ggcaggagtg acgttgatta tgatactcgt
     8281 gtctatttgg atgcatggta tgttaaaaac tggtctatgt ggaacgatat agctatcctt
     8341 tttaaaactg taggagttgt attaaaaaaa gatggcgcat attaaatcat cttaaaggtg
     8401 gaaaataaaa aagtttcgca tattacttat agattaaata gtgttttaat tattaacaaa
     8461 tgggtggtat gcgaactgca ttgtccatgg actatctatg aataaaaaag aagtaatcgc
     8521 tgttgtattt actgcagcaa tcgtcggtgg tcatgaattg atgacgattg ctcatttaaa
     8581 taaatttaga aataaagggt ttaggtttgc atgctttgtc ccttcagata atatagcgct
     8641 aaaaaaaatt ctcactgatc ataatatttc atatgagaat catgaagtgt tacataaaaa
     8701 atttgaaata cttcattcta cttttaatta tccttttcga aaaaaagcta aagaactact
     8761 agtatctctg aaggggaaac ataaaactat tatccttgtg caaggtgata ttgagttagg
     8821 ctctgtattt gttaatactt cgaaggctgc gggagtcaaa aacttagtta gttatatacc
     8881 ttatgcgcat agttttcgtt tgatgggtag caagttaggg tggttaaaag acatattatc
     8941 gaaatttgtt tacaagaagt gcgagaatta tattacgatt agcaaagttt ttgccgaaca
     9001 aatttccctt aaaaacaaaa atgccaaagt tagagtatta cataactttt cctctaagtt
     9061 accaatagaa tacattagag gtaagggata tatctataag aaagatgaga ataaatttac
     9121 tatcttaatg gcagggagag tttcatttag acaaaaagga caggatattt tacttcaggc
     9181 gttaaattct atttcgacct ctaaagaaat agtgcttaag gttattggag atggtcctga
     9241 tcttgagaag ctgaaaacta taacagaatg ttcacgtaat aatattagag taaattatta
     9301 tggctggcag ccaaatgtat gggagtatag caaggatgtt gatcttatta ccttgccatc
     9361 tcggtttgaa ggggtgccgc ttctgatgct agaagcaaag caagccaata tcccgattat
     9421 cgcccctgca cgtgatggaa tgattgattt tcttcctcca acaaatttat atgagccagg
     9481 tagtcgtgac tctgaagctg cggcactggc taaaaagatc cagactataa taaataacca
     9541 ctgaaatgta tcaaggaata tatgaaaaac attctaattg atgaccgatg gtcaggttca
     9601 ggcggaatcg gaacatttgc tgaagaaatt aataaaataa caaattataa gagtgcgggt
     9661 ttcaccggca aaccattcag ccctgttgat tgcataaaaa catcattaaa aatgatgaag
     9721 atgcgtgatg gtatagtttt ttttccaggc tatattcctc cactattttc cagagtacca
     9781 tttgtgttta caatccatga tttgaatcat ttggagcgtc ctgaaaattc aagtctatta
     9841 aaaaaaatat tctataaggt tgttataaaa agaggatgtc gaaaagcaaa gtatattttt
     9901 actgtttccg aattttctcg acaaaaaatc atccaatggt ccggggttcc tgctgataaa
     9961 gttataaatg tagggaatgg agtatccgac gattttcgac caaatggaca aaagaaagat
    10021 tttggatatc cttattattt atgtgtcagt aacaggaaaa gtcataagaa tgaggttgga
    10081 acaattcagg cgtttgccag agcagatatc cctaaaaacg taaaacttgt ttttaccggc
    10141 aagaaaaccg atgagttagc gactctcatc gaaatcaatg agcttactga cagggttgta
    10201 tttactggtt ttgtcaaaac cgaagaatta cctgagcttt atcgtggtgc agaaggattg
    10261 attttcgtct ccttctatga aggatttggt ctccctgtta ttgaggcaat ggcaagtggt
    10321 atacctgtaa ttacctccaa aacaacctcc ctcggtgagg ttgctggcga cgctgcactt
    10381 gtagttgacc ctgagaatat acagcaaata gcaactgcaa tatctaagtt acattctgat
    10441 attgaactta aaacccaact cattagtaaa ggctcaaaac aggtcggtaa atattcctgg
    10501 agtgctaccg cagaacttgt taataagtat ctaacaagta gttgagagta tagaaatcgt
    10561 aaaacaagaa tgaaattttt ctgtgttgac gataatgttg gtaaaaatga gtgaaaaata
    10621 cgctacaaat aaaaaaatag ttgcaaagaa cacattcatg ctttatggca ggatgttact
    10681 aataatgttt gcaacttttt atacctcacg cttagtttta aatgcgcttg gggtttatga
    10741 ctttgggctt tacagtgtca ttggcagtat tatcgtttta ttttcgttca ttcaaaacgt
    10801 atcagctttg gcaactcaac ggttcctgtc agttggttta ggtagaaggg acttttcctg
    10861 gactaaaaga gtcttttcga caagtatatt ggtccatttt ataatatgtg gcgctgtttt
    10921 attcctcgct gaaacaattg gtttatggtt cttaatctat aaattaagag ttcccgttga
    10981 gcgtatgaca gaaatatttt gggtgtacca gttgtctgtc atatctctgc ttattcagat
    11041 catgcaaaca ccatttatcg ctgcattaat tgcaactgaa aagatggatt tgtttgctaa
    11101 aatagggata tttgatgcta ttcagcgttg gggcatggtg tatttattta ccttgttcga
    11161 attcgattcg aaattaatat ggtatgctgc attcttgctt tttggatata tatcagtgtt
    11221 ttttgtctat tttgttagtt gcattaagaa aatagatata tgtactgtaa aggtaagttt
    11281 caaagaaaat aaagaactgc tcaaggaaat actatccttc tcgagttggt ccatgatggg
    11341 ctcattgagt ttagtcggac tttctcaagg tattgctctc ctgacatatt tctttgtcgg
    11401 agttgtggca aatggttctg tttggttagc ggagcaggtg ctgattgcat ttaaccgtgt
    11461 cataggtact ttgcaaaccg cattcaatcc tcaactaata aagcagcatt caactggaaa
    11521 tcacgaagag gtttcgtctc ttctatcttt atcctgcaag ttgactggct ttatcgtact
    11581 gattgcagca gtacccgcct ttgtagatgc gccttttgtt attaacttat ggttgaaaca
    11641 agttccagaa catttggtgg tgttggtaag aattgtcata ctgtatgtca tcattgattc
    11701 tctatcagga ccttttgtta ccgtagcata tgcagtagga aagttgcgga actatcagtt
    11761 aatagcatca ttaataatgc ttttaacttt agtattaaca tatgctcttt actctttggg
    11821 ttttgcattg gttgtggcgg tgtcgagtcg tatactttgt gcagttgcaa tgcttatgtt
    11881 cagagttttg tatgtggaaa aagtaactgg attaaatagt aaaatatttt tccttaatat
    11941 tttcccgcgc ttattttctg tttctgtaat atcatttgca atttcatttt atattaacac
    12001 cgtacttcct gtaggttttt taggattact gcttctaatc ggcttaaaca ctatcgcagt
    12061 ggctatactc tttttctatt tggtgctaat tagttcagaa agaaaatatt taacatcttt
    12121 aataaagaaa aaattaggta ggaaataaca tgcaagtaaa caatactcaa tatggcgtcc
    12181 tgacgtacga aagtgacatc tatgtcaaaa gaaactggat caatctgggt gattatgtac
    12241 aaagtacagc agcacgccag tttttacctt gcgtgaatga gtatatacca cgagatcata
    12301 tgaatactta tagtggtaat aaagtaaaaa tgatcatgaa tgcgtggtat atggatatac
    12361 cagaaaactt tccgccatct gaagatataa ctcctttata tgtgtcaatc catattaact
    12421 ctactattgt agataaaatt tttacacctg cgagtattga gcactttaaa aaacatgcgc
    12481 cgataggttg ccgtgatttt tacactcgtg atttgctgat ctcaaaaggg attgatgcgt
    12541 attactcagg ctgtatgaca cttacgttgg ggaataaata taaacgggat aatgttacgg
    12601 atgatattta ttttatcgat gtaatgtatg actcaatgtc attaccagag ttaatgagcc
    12661 aaccattacg tttcggaaaa cgcttgctaa atggtcgggc atttgaattt aatcatagaa
    12721 gcaaagtact aaatcagttt tttgatagtg aattactcaa gcatgctaaa catgaaaaac
    12781 agatcattcc gtacctttcc gctgacgaag gttttaaatt agctgatgat tttctgagga
    12841 aattggctaa tgcaaaactg gtagtaacct ccaggattca tactgctctt ccatgcctgg
    12901 ctatgggcac tccagttatt tttgtaaatg gtggttttaa aaataaagtt gataactgcc
    12961 gtttcgatgg attatttgac ttctttaata gaattgacgt cgatatgaat gccaatgcga
    13021 ctgctaattt cgaatttgat ggtaagaaaa taggtatgaa tactattatt gaaaataaaa
    13081 cattgcatga acaatatgct aatgaattag cggataaatg cagaagtttt gttgtgtaat
    13141 tttattctaa atgtctctat atcgaactgt aaatatatta aatgaggtta agtcatgatt
    13201 ctcccagtga taatggctgg aggtactggt agtcgtttat ggccgatgtc gcgagagcta
    13261 tatccaaaac agtttttacg tttacacggc gagcaatcaa tgcttcagga gacaattact
    13321 cgccttaatg gtttagagac taaagaacct attgtgatat gtaacgaaca acatcgtttt
    13381 ttagtcgcag aacaactacg tcaaatcgaa aagctatcta ataacattat ccttgaacca
    13441 attggacgta atacggctcc agcaattgca cttgctgcat tacacgcagt agcacatgat
    13501 gatgatgatc cattgctact tgttttggcc gcggaccacg ttattgagaa taacgctgct
    13561 tttcaagctg caatcaacag cgctgtagtt tatgcaaatc aagggaagct tgtaacgttt
    13621 ggtattgtgc ctactggtcc tgaaacggga tatggttata tccagcgtgg taacgaagag
    13681 catttcgata atcatatagc ttatgatgta aaacgtttcg ttgaaaaacc taacttgcat
    13741 acagcacaag aatatttgga cagcggtgaa tattactgga atagcggtat gttcatgttt
    13801 cgagcaagcc gctttctgca agagttggat gagttccgtc cagatattct tagctcatgt
    13861 aaaaaggcaa tgcagactgt gcataccgat tctttacgtg attttattcg tgttgataaa
    13921 gatgctttta ttgcttgccc tgatgagtca gttgattatg ctgtaatgga aaaaacgcaa
    13981 tctgcatctg tagtaccact ggatgcaggt tggaatgatg ttggttcatg gtcagcactg
    14041 tgggaagtca acgataagga tggtcaaggt aattcactat ccggggatgt ttttgttcat
    14101 gattcttcta actgttatat aaatagcgaa gaacaactag tagctgccgt tggggttaac
    14161 aatctggtgg tggtcagcac caaagatgct gtgctcgtta tagataaatc acaggtgcaa
    14221 gacgttaaac gtgtagttga acatcttaaa atgacttcac gtagagagta taggatccac
    14281 cgtgaatcct atagcccttg ggggcatagt gataaagtgg ttaatacaac taaatatcac
    14341 attaacaaag ttacagttaa accgggcggt aagttttctt tgcaaatgca ccaccaccgg
    14401 gccgagcact ggattatatt atcaggaaca gcgaagatta ccttaaacga acgtgagttt
    14461 ttgcttactg aaaaccaatc aacatttatt cctgctggtt cgactcatat gctggaaaat
    14521 ccagggaaaa tagatcttga aatccttgag attcaatctg gttcatattt aggtgaagat
    14581 gatattgttc gtctaaaaga tcattatgga atttgattaa acggggaaaa tgatggaatt
    14641 attaacctgc tttaaagcgt atgacattcg cggcaagtta ggctcggaac ttacagagga
    14701 catcgcatac cgtattggtc gtgcatatgg tgagtatctt aagcctaaaa aaatggttgt
    14761 cggaggcgat gttagactaa ctagtgaatc ccttaaactt gcccttgcca aaggattgca
    14821 ggatgctggt actgatgttt tagacattgg tttatcgggt actgaggaga tttattttgc
    14881 tacatcttac ctcggcgttg atggcggagt ggaagtaaca gccagtcata acccaattga
    14941 ttataatgga atgaaactcg tccgcgagaa ctcaaagcca atcagtggtg atactgggct
    15001 aagggagatc cagcgtcttg ccgaagagaa taattttgct ataaattcat cacagcctaa
    15061 aggtagttat caatccattt ctgtattaga tgcatatatt gataagttat tgagctatat
    15121 caatcttcat aatatcaata gaacgctaaa attagttgtt aactctggta atggcgccgc
    15181 tggtcatgtt attgatgccc ttgagaagcg ctttataaaa cataatgtcc ctgttgaatt
    15241 tataaaggtg caccatgaac ccgatgggac cttcccaaat ggtataccaa atccattatt
    15301 accagagtgt agaaaagata ccactgacgc tgtcataaaa aataatgctg atatgggtat
    15361 tgcattcgat ggtgatttcg atcgatgctt tctgttcgat aatatgggta actttatcga
    15421 aggctactat attgttggtc ttttggctga cgcatttctt gaaaagaatc ctggttctcg
    15481 tattattcat gacccgcgtt taagctggaa tacaattgat attgtaaaaa atgctggcgg
    15541 tattcctgtg atgtctaaaa caggtcatgc tttcattaaa gaacgtatgc gtgcagaaga
    15601 tgcaatatac ggcggcgaaa tgagcgccca ccattatttc cgcgattttg cctattgcga
    15661 tagcggaatg ataccatggc tccttgtcgc tgagttactc tgtatcaagg ataaaacgct
    15721 aaaccaatta gttagtgcac ggatgcaagc ttatcctgcc tctggagaaa tcaacagctc
    15781 ccttagcgat ccacaatcct cgatcaaacg ggttcttgag cgctatcaga atgaagctct
    15841 tgatattaat tacacggatg gtataagcct tgaatttgcg gactggcgct tcaatctacg
    15901 tagttctaac actgagcctg tcgttcgctt aaatgtggaa tccagaagtg atgttgcttt
    15961 aatgaaagaa aaaactgcag agagtttagc cttgttaagg gcttaaattt aaattactaa
    16021 aggtgctttt caaatctgga gcacctttat attttgcatg ttaaattata aatgtacgat
    16081 ggatttttat gcgcgattta aatgttggta ttgttgctga ttggtttatt acttatgctg
    16141 gttctgaaaa agtagtagcg gaacttatta aatcatatcc agaatcacac ttgtattctg
    16201 ttgtggattt tctttcctct gaaaataaaa aacactttca gaataaagaa ataaccacaa
    16261 cattcattca acgcctccca aaagcgaaga aaaaatatca aacatatctt cctttaatgc
    16321 cattagcgat tgagcaactt gatgtatcca gccatgatgt cattctttct agcagccatg
    16381 ctgttgctaa aggggttttg acgggacccg atcaactgca tattagttat gttcattccc
    16441 ctataagata tgcatgggat ctccagcatc aatatctgcg ggaagcaggt ttagatcatg
    16501 gagtcaaagg aatgattgca aaattgattt tgcataaaat gcgcatgtgg gactaccgta
    16561 ctgcaaatgg cgttgatcat tttattgcta actcacaatt tatcgcacgg cggattaaaa
    16621 aagtttatgg gcgagaggct gatgtcattt atccgcctgt tgatgtcaat cgctttcttc
    16681 tgaatgaagc taaagaagat tattacttta ccgcatcacg ccttgttcca tataagcgta
    16741 ttgatttgat agttgaggct ttcagtcaca tgccaaaccg taaacttgtt gttgttggtg
    16801 atgggccgga aatgcaaaag ataaagtcta aagccacggc taacattgaa atcctgggct
    16861 atcaatcaaa tgaagtcatg caagagcata tgagaaatgc taaagcattt gtttttgcgg
    16921 cagaagaaga ttttggtatt actcctgttg aagcgcagtc ttgtgggacg cctgtaattg
    16981 cgtttggtaa aggtggttca cttgaaacag taagaccatt tggtgtagat aatccaacag
    17041 gtatcctttt tgacaaacag gatatagatt ctgtcattga tgctgtacaa aagtttgaaa
    17101 ctatgcagga tcaaattctt cctaatgatt gtcgtgctca tgcgttaaaa ttttcaaccg
    17161 agcggtttaa tcaggaaatt tctgaatacg tcaattctaa atattgcgaa tttgtggaaa
    17221 ataaaaaaat catctattaa
//
