LOCUS       GU299791               10301 bp    DNA     linear   BCT 08-JUN-2010
DEFINITION  Escherichia coli strain G1632 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299791
VERSION     GU299791.1  GI:288816197
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and note,L.
  TITLE     A multiplex PCR method to detect 14 Escherichia coli serogroups
            associated with urinary tract infections
  JOURNAL   J. Microbiol. Methods 82 (1), 71-77 (2010)
   PUBMED   20434495
REFERENCE   2  (bases 1 to 10301)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and note,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1632"
                     /db_xref="taxon:562"
                     /note="serogroup: O1"
     misc_feature    1..10301
                     /note="O antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADC54922.1"
                     /db_xref="GI:288816198"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGQAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LANAKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADC54923.1"
                     /db_xref="GI:288816199"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLEMDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARNAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADC54924.1"
                     /db_xref="GI:288816200"
                     /translation="MKTRKGIILAGGSGTRLYPVTMVVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGVGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFVDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3033..3581
                     /gene="rmlC"
     CDS             3033..3581
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADC54925.1"
                     /db_xref="GI:288816201"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSSTFGKWVGVN
                     LSAENKRQLWIPEGYAHGFLVLSETAEFLYKTTNYYHPQSDRGIKWDDPSINISWPVD
                     SQVLLSAKDNKHPPLTKIEMYS"
     gene            3625..4851
                     /gene="wzx"
     CDS             3625..4851
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54926.1"
                     /db_xref="GI:288816202"
                     /translation="MSKSEIRNVIHNAGYMMITQIALYVAPLFILSYLLKTLGVAQFG
                     NYALILSIVAYLQIITDYGFSFSASRAISQNREDKEYISKIYLSTMTIKLAICAFLFL
                     LLMLFLNLLPVQAELKQGILYGYLLVIGNTFQPQWFFQGIEKLKIIALSNVISRCAAC
                     LLVFIYVRNSEDLQKALLVQSLPLVISAIGLNIFILKYINIIFPEKKLFKVILKEGKD
                     FFLASLYSVILNNSGIFLLGIFTNPVIVGVYAAAEKIVKAVLSLFTPLTQAIYPYNCR
                     KFSLSVFDGIEAAKKTGIPIIILAFIAAVIVAITLPVAIDYLNFPKETIFVGQILSAW
                     IFFGVLNNVFGIQILSASGRSKIYSRMVFVSALITLLLITLLLQFCNATGVACAILLG
                     EMFLSILLLKRYKKII"
     gene            4861..5988
                     /gene="mnaA"
     CDS             4861..5988
                     /gene="mnaA"
                     /note="UDP-N-acetyl glucosamine-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="MnaA"
                     /protein_id="ADC54927.1"
                     /db_xref="GI:288816203"
                     /translation="MKKLLLVFGTRPEAIKMASIIELLKKDCRFEYKICVTGQHKEML
                     DQVMQVFDVKPDYNLRIMQPGQTLVSVATNILSRLSEVLIIEKPDIILVHGDTTTTLA
                     ATLAGYYHQIKVCHVEAGLRTGDIYSPWPEEGNRKVTGALACIHFAPTERSKDNLLRE
                     GVKVNNIFVTGNTVIDSLFIAKDIIDNDPNIKNALHNKFNFLDKSRRVVLITGHRREN
                     FGKGFEDICFAIKELAFIYPNVDFIYPVHLNPNVMEPVHRILDNICNIYLIEPLDYLP
                     FVYLMNESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVVLVGTSKIKIV
                     NKVTELLNNADIYNAMSLLHNPYGDGTAAQKILNVLAQELI"
     gene            6003..7067
                     /gene="wekM"
     CDS             6003..7067
                     /gene="wekM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuV"
                     /protein_id="ADC54928.1"
                     /db_xref="GI:288816204"
                     /translation="MLLIIADYPNEMNMREGAMQRIDAIDSLIRDRKRVYLNISFKKH
                     LVRSNSSFNNVIVENLNAIIHRNIIKQYMQKSTTIYVHSVYNLLKVITLIDLKKTILD
                     IHGVVPEELLADNKKLLSKVYNMVEKKGVLGCKKLIHVSTEMQKHYEAKYGVNLAERS
                     IVLPIFEYKNITQSQNKWTENKIRSIYLGGLQTWQNIDKMIQVCDDTVINNEAGKYEF
                     NFFIPQSNLEGFIDKYSLKLHNINANASTLSRDEVIPFLKECHIGFVLRDDIIVNRVA
                     CPTKLVEYLECGVVPVVLSPLIGDFYSMGYQYITTEEMANRSISLLDLEKMAAHNLQI
                     LTSYQKRTYKAQKELIAQLC"
     gene            7119..8123
                     /gene="wzy"
     CDS             7119..8123
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54929.1"
                     /db_xref="GI:288816205"
                     /translation="MRIKYKDNGYILCFLLCFILSYLVLLKSDYFPADFLPYTEIYDG
                     TYGEINNIEPAFLYLTRLFHYLNFPYIFFAMLVCALCLSWKIKYARKIIKDSYIYLFL
                     YVYVSFYVFLHEMTQLRIAIAVTMCYVSVYYYFYKNCVKHALPWMVLAILFHYSALLL
                     FMSLFIYSYRGLLIVIIGFVICMSFLNVYADTIALYLPNEKIVNYLYSISSSLDNRND
                     LAIFNLNNIIFLSIFILIFYLSRYIKLNDNEAKFIKYVQCSGILAFCIFFLASGVPVI
                     AYRTAELLRIFYPMALVLILSHIKNNNMRYFIAVIIVILSGLMLFITLRAVSIVGQGL
                     "
     gene            8125..9084
                     /gene="wekN"
     CDS             8125..9084
                     /gene="wekN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekN"
                     /protein_id="ADC54930.1"
                     /db_xref="GI:288816206"
                     /translation="MNVAILLSTYNGEKYLEEQLDSLLLQSYQDFVVYIRDDGSSDRT
                     VNIINQYVMKDNRFINVGNSENLGCAASFINLLRNASADIYMFCDQDDYWLPNKLQRA
                     VDYFSAIDPLQPTLYHCDLSVVDEKLNIIQNSFLQHQKMSAYDSMRKNNLFIQNFVVG
                     CSCAVNASLAEFVLSRIGEQHVKMIAMHDWWLAVTAKLFGRIHFDNTQTILYRQHQGN
                     VLGAKSSGMMRFIRLGLNGQGISRVVSFRKKVCAQNKLLLDVYDKDLNLEQKKSIRLV
                     IEGLKENSSIADLLKCFYHGSYMQGFKRNLALIYSVLYTKKRR"
     gene            9094..10158
                     /gene="wekO"
     CDS             9094..10158
                     /gene="wekO"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekO"
                     /protein_id="ADC54931.1"
                     /db_xref="GI:288816207"
                     /translation="MKKIAIIGTVGIPASYGGFETLVENLTRYNSSGVEYNVFCSSFH
                     YKSHQKKHNGARLIYIPLKANGWQSIAYDIISLAYSIFLKPDVILILGVSGCSFLPFF
                     KLLTRAKFITNIDGLEWRRDKWNSKVKRFLKFSEKIAVQYSDVVITDNEAISEYVFNE
                     YNKDSRVIAYGGDHAWLNTEDVFTTRNYKSDYYLSVCRIEPENNVELILKTFSKLKYK
                     IKFIGNWNGSEFGKKLRLHYSNYPNIEMIDPIYDLQQLFHLRNNCIGYIHGHSAGGTN
                     PSLVEAMHFSKPIFAYDCKFNRYTTENEACYFSNESDLAEKIIMHCELSLGVSGTKMK
                     EIANQKYTWRRIAEMYEDCY"
BASE COUNT     3237 a   1632 c   2102 g   3330 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aagatacttg
      121 ttactggggg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ctctgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct catcctgacg
      541 aagtaaataa taaagaacaa ttacccctct ttactgagac gacagcttac gcgcctagta
      601 gtccttattc cgcatcaaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gactattgtg actaactgtt cgaataacta cggtccttat cactttccgg
      721 aaaaattgat tccactagta attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtagttac tgaaggtcaa gcgggtgaaa cctataacat tggcggacac aacgaaaaga
      901 aaaacatcga tgttgtgctg actatttgtg atttgttgga cgagatagtc ccgaaagaga
      961 aatcttatcg tgagcaaatt acttatgttg ctgatcgccc agggcatgat cgccgttatg
     1021 cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gtgggattcg taaaacggtg gaatggtatt tggctaatgc aaaatgggtt gataatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     1261 gggtaatttg attgctcttg atgttcactc cactgattac tgtggtgatt ttagtaaccc
     1321 tgaaggtgtg gctgaaacag tcaaaagaat tcgacctgat gttattgtta atgctgcggc
     1381 tcacaccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 tagcgttgaa tcaattgcaa aagcggcaaa tgaagttggg gcttgggtaa ttcattactc
     1501 aactgactac gtattccctg gaaatggcga cacgccatgg ctggagatgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agctggagaa aaagcattac aagagcattg
     1621 tgcgaagcac ctaattttcc gtaccagctg ggtctatgca ggtaaaggaa ataatttcgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg tgaagaacta gccgttatta atgatcagtt
     1741 tggtgcgcca acaggtgctg aactgctggc tgattgtacg gcacatgcca ttcgtgtcgc
     1801 actgaataaa ccggatgtcg caggcttgta ccatttggta gccagtggta ccacaacctg
     1861 gtacgattat gctgcgctgg tttttgaaga ggcgcgcaat gcaggcattc ctcttgcact
     1921 caacaagctc aacgcagtac caacaactgc ctatcctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaattttgcg cttgtattgc ctgactggca
     2041 ggttggtgtg aaacgcatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tactcgtctt tatcctgtga ctatggtcgt
     2221 cagtaaacag ctattaccta tatatgataa accgatgatc tattatccgc tttctacact
     2281 gatgttagcg ggtattcgcg atattctgat tattagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgtcg gtagccagtg gggcctgaat cttcagtaca aagtgcaacc
     2401 gagtccggat ggtcttgcgc aggcatttat tatcggtgaa gagtttattg gtggtgatga
     2461 ttgtgctttg gtacttggtg ataatatctt ctacggtcac gacctgccta agttaatgga
     2521 tgccgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta atgatcctga
     2581 acgctatggt gtcgttgagt ttgataaaaa cggtacggcg atcagcctgg aagaaaaacc
     2641 gctacaacca aaaagtaatt atgcggtaac cgggctttat ttttatgata acgacgttgt
     2701 cgaaatggcg aaaaatctta agccttctgc ccgcggtgaa ctggaaatta ccgatattaa
     2761 ccgtatctat atggaacaag ggcgtttatc tgttgccatg atggggcgtg gttatgcgtg
     2821 gttagacacg gggacacatc agagcctgat tgaggcaagc aactttattg caacaattga
     2881 agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gcttaccgta aagggtttgt
     2941 tgatgctgag caggtgaaag tattagctga acctctgaaa aaaaatgctt atggtcagta
     3001 tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaaca gaaattcctg
     3061 atgtactgat ttttgaaccg aaagtttttg gtgatgagcg tggtttcttt tttgagagct
     3121 ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtt caggataacc
     3181 attcgaagtc tagtaaaggt gttttacgcg ggctgcatta tcagttggaa ccttatgcac
     3241 aaggaaaatt ggtgcgttgc gttgtcggtg aagtttttga cgtagctgtt gatattcgta
     3301 aatcgtcatc gacttttggc aaatgggttg gggtgaattt atctgctgag aataagcggc
     3361 aattgtggat tcctgaggga tatgcacatg gttttttagt gctgagtgag acggcggagt
     3421 ttttgtataa gacgacaaat tattatcatc ctcagagtga tagaggaata aaatgggatg
     3481 atccaagcat caatatttca tggccagtcg attcacaagt gctgctatca gctaaagata
     3541 ataagcatcc tccattaaca aagattgaaa tgtatagtta agatcacgat aaatcttgga
     3601 agggttgcaa aattgaataa aatagtgagc aaaagtgaaa taaggaacgt aatccacaat
     3661 gctggctata tgatgattac tcagatagct ttatatgttg caccattatt tatactgagt
     3721 tatctgttaa aaacactggg ggttgcacag tttggtaatt atgccttaat actatcaatc
     3781 gttgcatatt tacagattat aacggattat ggtttttctt ttagtgcaag tcgtgcgatc
     3841 tcacagaata gagaggacaa agaatatata tcaaaaattt atctgtcaac tatgactatc
     3901 aagttggcga tatgcgcttt cttattctta ttgctcatgc tatttttaaa tcttttgcct
     3961 gtgcaagctg aattaaaaca aggaatatta tatggatatc ttcttgtaat aggaaatact
     4021 ttccaaccac aatggttttt ccaaggtatc gaaaaattaa aaatcatagc cctttctaat
     4081 gttatatcaa gatgcgccgc gtgtttactt gtatttatct atgtgaggaa tagcgaggat
     4141 ttacaaaaag cacttttagt acagtcactt ccattagtaa tttctgcgat tggattaaat
     4201 atatttatat tgaaatatat caatattatt tttccggaaa aaaaattatt taaggtaatt
     4261 ttaaaagaag gtaaggattt ttttcttgca tcactttatt ctgttattct caataatagt
     4321 ggcatttttc tattagggat ttttactaat cctgttattg ttggtgtata tgccgccgct
     4381 gaaaagatag tcaaggccgt attgtcgcta tttacaccac tgacgcaagc tatatatcct
     4441 tataattgtc gtaagttttc actatccgta tttgacggca ttgaggcagc aaaaaaaact
     4501 ggtataccaa ttataatttt agcatttata gctgctgtta tcgttgcaat taccttacct
     4561 gttgcaatcg actatcttaa ttttccaaaa gaaacaattt ttgtaggtca aatattaagt
     4621 gcatggatct tttttggtgt tcttaataat gtattcggca ttcagatatt gagtgcatca
     4681 ggaagaagta aaatatatag taggatggta ttcgtatcag cgcttataac attacttttg
     4741 attactctat tattgcagtt ttgtaacgcc actggagtgg catgtgcaat attattgggt
     4801 gaaatgttct tatcaatatt gttacttaag cgatataaaa aaataattta aggaatagtt
     4861 atgaagaagt tattattagt gttcggtact aggcctgaag caataaagat ggcctctatc
     4921 attgaattat taaaaaaaga ttgtagattc gaatataaaa tatgtgtgac aggccaacat
     4981 aaagagatgc ttgatcaagt tatgcaagta tttgatgtta aacctgatta taatttacgg
     5041 attatgcagc ctgggcaaac attagtatct gtagcaacaa atatactctc acggttaagt
     5101 gaagttttaa ttatagaaaa gccagatatt atacttgtgc atggggatac aacgactacc
     5161 cttgctgcta ctttagctgg gtattaccac caaataaaag tttgtcatgt ggaagcagga
     5221 ttaagaacag gggatattta ctctccttgg cctgaagagg gcaatcgtaa agttacaggg
     5281 gcattagcat gtattcattt cgccccaaca gagagatcaa aagataatct cctgagggag
     5341 ggggtcaaag taaataatat atttgtaacg ggtaataccg tcatcgactc tttatttatt
     5401 gcaaaagata tcatagataa tgaccctaat ataaagaacg ctttacataa taaatttaat
     5461 tttcttgata aaagccgacg agtagtactt ataacaggtc atcgaagaga aaatttcggg
     5521 aaaggttttg aagatatatg ctttgcaata aaggaattag ctttcattta tcctaatgta
     5581 gattttattt atccggtgca tcttaatccc aatgtaatgg aaccagtaca tcgtatatta
     5641 gataatatat gtaatattta ccttattgag cccttggatt atttgccttt tgtttattta
     5701 atgaatgagt catatttaat attgactgat tcagggggga tacaagaaga agcgccttcg
     5761 ttaggtaaac cggttttggt tatgcgtgat actactgaac gccctgaggc ggttgaggct
     5821 ggtactgttg tattagtggg gacttctaag ataaaaatag taaataaagt aacggagcta
     5881 ttaaacaatg ctgatatcta caatgctatg tctctgttac ataatccata tggcgatgga
     5941 acagctgctc aaaaaattct taatgtgctc gcccaagagc taatttaatt taagctaaaa
     6001 atatgttatt aattattgct gattatccaa acgaaatgaa tatgcgcgag ggagctatgc
     6061 aacgaataga tgcgatagac tctctcattc gagatcgcaa gcgagtgtat ttgaatattt
     6121 cattcaaaaa gcatctagtt cgctcaaata gttcctttaa taatgttata gttgaaaatc
     6181 taaatgcaat tattcacaga aacatcataa aacagtacat gcaaaaatca acaactatat
     6241 atgttcattc tgtttataat ttattaaagg ttataacgct cattgatcta aaaaaaacaa
     6301 ttcttgatat acatggtgtt gtaccggaag aacttttggc agataataaa aaattactta
     6361 gtaaagtata taacatggtg gaaaaaaaag gtgtccttgg atgcaaaaaa ttaatacacg
     6421 tcagtacaga aatgcaaaaa cactatgaag caaaatatgg agtaaacttg gctgaaaggt
     6481 caatagtgct cccgattttt gaatataaaa atataaccca atcgcaaaac aaatggacag
     6541 aaaataaaat acgaagtatc tatcttggag gattacaaac atggcaaaat attgataaaa
     6601 tgattcaagt ttgtgatgac acagtgataa acaatgaagc aggtaagtat gaattcaact
     6661 ttttcatccc acagagtaac ttggaagggt ttatagataa atattcgtta aaattacata
     6721 atatcaatgc taatgcatct acgctatcac gtgatgaagt aattcccttt ctaaaagaat
     6781 gtcatattgg ttttgtattg cgcgatgata taatagtaaa cagagttgcg tgccctacaa
     6841 aattggttga atatttagag tgtggtgtcg ttccagttgt gctctcccca cttataggtg
     6901 atttttattc gatgggatat caatacatta ctacagagga aatggctaac agaagtataa
     6961 gtttgttgga tcttgaaaaa atggctgcac ataatttaca aattttgact tcttatcaga
     7021 agagaaccta caaggcacag aaagaactta ttgctcaact gtgctgaatt ttttacatat
     7081 ataaaattat gtaagcatat cgcgggtcag gtaattgtat gcgtatcaaa tataaagata
     7141 acggttatat attatgtttt ctattatgtt tcattttgag ctacttagtt ttactcaaat
     7201 ctgactactt tcctgctgat tttctgccat atacagaaat atacgatggg acatacggag
     7261 aaatcaataa tattgagcct gcctttttat atttaacacg gttgtttcat tatttaaatt
     7321 tcccctatat attttttgca atgttagttt gtgccttatg tttaagttgg aaaataaaat
     7381 atgcaagaaa aataattaaa gatagttata tatatttgtt cttgtatgta tatgtatcat
     7441 tttatgtgtt tttgcatgaa atgactcaat tgcgcatagc aattgcagtc actatgtgct
     7501 atgtgtcggt ttattattac ttttataaaa attgtgttaa acatgcactg ccatggatgg
     7561 tgttggctat tttgtttcat tacagcgcct tgcttttatt tatgtcatta tttatataca
     7621 gttatagggg gttattaata gtaattatag ggtttgtaat atgtatgagc tttttaaacg
     7681 tgtatgcaga tacaattgca ctatatttgc caaatgaaaa aatagtaaat tatttatata
     7741 gtatttcatc atcattagac aatagaaatg atttggcaat attcaacctg aataatataa
     7801 tatttttatc aatatttatt ttgatctttt atcttagccg atatataaaa ttaaatgata
     7861 atgaggcgaa gtttattaag tatgtgcaat gttcaggaat attagccttt tgtattttct
     7921 ttctggctag tggagtcccg gtcattgctt atcgaactgc agagttgctg cgaatatttt
     7981 atccgatggc tttagtatta atcctttcgc atataaaaaa taataatatg cgttatttta
     8041 ttgcagtcat tatagttatc ctttcaggct taatgttgtt tataacacta agggctgtat
     8101 caatagttgg tcaaggatta taaaatgaat gttgctattt tgttgtctac gtataatggc
     8161 gaaaaatatt tagaggaaca actggattca ttgctgcttc aaagttatca ggattttgta
     8221 gtgtatatcc gtgatgacgg atcatctgat agaactgtaa atataataaa ccaatacgta
     8281 atgaaagata acagatttat taacgtgggt aattcagaaa atcttggttg tgctgcttcg
     8341 tttattaatt tattaagaaa tgcttcagcc gatatttata tgttttgtga ccaagatgat
     8401 tattggcttc cgaataaatt acagcgtgct gtggattatt tttcggctat tgatccttta
     8461 caacctacct tgtatcattg cgatctaagc gttgttgatg aaaaacttaa tattatacaa
     8521 aattcatttt tgcagcatca gaaaatgtca gcgtatgatt caatgagaaa aaataatctt
     8581 ttcatacaaa attttgttgt tggttgttca tgtgctgtta atgcttcact tgcggaattt
     8641 gttctttcgc gaattggaga gcagcatgta aaaatgatag ctatgcatga ctggtggtta
     8701 gccgtgactg caaaactttt tggtcgaatc cattttgata atactcaaac gattctttat
     8761 cgacaacatc agggcaatgt attaggtgca aaatcatcag gtatgatgcg ttttattcga
     8821 ttaggattaa atgggcaagg gatttcgcga gtagtatctt ttagaaaaaa agtttgtgcg
     8881 caaaataagc ttcttttaga tgtctatgat aaagatttaa atcttgagca aaaaaaatct
     8941 atcaggcttg taattgaggg ccttaaagag aactcttcaa ttgctgacct tttaaaatgt
     9001 ttctatcatg gtagctatat gcaaggtttt aaacgtaatc ttgccttaat atattcagtt
     9061 ctttacacaa aaaaaagaag atagtgtatc cttatgaaaa aaattgctat tatcggtact
     9121 gttggcatac cagcatcata tggcggattt gaaacattag ttgaaaattt aacaagatac
     9181 aattcctcgg gagttgaata taatgttttt tgttcatcgt ttcactacaa atcccaccaa
     9241 aaaaaacata atggggcccg tttaatttat attccgctta aagccaatgg atggcagagc
     9301 attgcgtatg acataatttc gttagcatat tctatttttt tgaagcctga tgtgattctg
     9361 attttagggg tttctggttg ttcatttttg cctttcttca aactcttaac acgcgctaag
     9421 tttattacta atattgatgg cctggaatgg cgaagagata aatggaattc aaaagtgaaa
     9481 cgtttcttaa aattttcaga aaaaatcgca gttcaatatt cggatgtcgt tattacggat
     9541 aatgaggcaa tttctgagta cgtttttaac gagtataata aagatagccg agttattgcc
     9601 tatggagggg atcatgcatg gttaaatact gaggatgtat ttacaacaag aaattataaa
     9661 agcgattact acctttctgt atgtcgtatc gaacccgaaa acaatgtaga attaatttta
     9721 aaaacatttt caaagctaaa atataaaata aaatttattg gaaattggaa tggcagcgag
     9781 tttggaaaga aacttaggct gcattattct aactatccaa atattgaaat gattgatccg
     9841 atttatgatc ttcaacaatt atttcactta cgaaataatt gcataggata tatacatggt
     9901 cattcggctg gaggaacaaa cccttcttta gtcgaggcaa tgcattttag taaacctata
     9961 tttgcatatg attgtaagtt taataggtac actactgaaa atgaagcatg ttatttttct
    10021 aatgaatctg acctcgcaga gaaaatcata atgcattgtg agctatcatt aggtgtctct
    10081 ggcacgaaaa tgaaagaaat tgctaaccag aaatacactt ggagacgaat agcagaaatg
    10141 tatgaggatt gctattaact ctgttaaact tcaaatcttt tacaatatat ggcatgacta
    10201 taagcgcatt aattgttttt caagccgctc tcgcggtgac caccccctga caggagtaaa
    10261 caatgtccaa gcaacagatc ggcgtcgtcg gtatggcagt g
//
