LOCUS       AB812046               26407 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H510a.
ACCESSION   AB812046
VERSION     AB812046.1  GI:733166924
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 26407)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..26407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H510a"
                     /db_xref="taxon:562"
                     /note="serogroup:O101"
     gene            <1..866
                     /gene="wcaM"
     CDS             <1..866
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01466.1"
                     /db_xref="GI:733166925"
                     /translation="GANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGK
                     INWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARN
                     ITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVN
                     NIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINVMQE
                     SSVGSALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVE
                     KINFRLPQRRE"
     gene            1028..1933
                     /gene="galF"
     CDS             1028..1933
                     /gene="galF"
                     /note="O101_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01467.1"
                     /db_xref="GI:733166926"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVIIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWSELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLGK"
     CDS             2376..3392
                     /note="O101_02"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ01468.1"
                     /db_xref="GI:733166927"
                     /translation="MTILVTGGAGYIGSHTVLQLIEGGYDVVVLDNLSNSSQESLHRV
                     GSLVSKNIPFIKADINDKQKLEEVFKNYDIDAVIHFAGLKSVGESVKKPLEYYINNVS
                     GTLTLLEVMKQANVYKFIFSSSATVYGNHSPVPNLEEYPIGNASCPYGTTKVMLEQVL
                     SDYAKSDDKLKIIALRYFNPTGAHPSGLIGEDPNGIPNNLVPYISQVAIGKLEKLSVY
                     GGDYETIDGTGVRDFIHVVDLAAGHVSALRYIDKLQGYDIFNLGTGTGVSVLQVIDAF
                     EKASNQKVNYEIVARREGDIGSSWADVSKAERILGWKSKYNIEDMMVHTWNWQKQNPN
                     GYGS"
     CDS             3426..5642
                     /note="O101_03"
                     /gene="werR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01469.1"
                     /db_xref="GI:733166928"
                     /translation="MNLFFIVDKLDWAFGRIAQNIATELSSFNVKIVCTDDFKTVADF
                     AMHMDKNVKEKSILHFFWRDYLMEVMNFTQRNPQYKEILIRNIITTHIPDHLFTYDDI
                     ANYERRIPLMNFVDGYFVTSGKLKELYSELSDFPPPHSVIYDNPEIPMSEVKSYDNHD
                     RKKIVWIGNSKWGEYLGYQDYKGLNTIVLPALDKLKKSGYTFDYIEFDSSKGKTDREV
                     ILKTLSDADILLISSKCEGTPLPLLEAMANSCAVVSTDVGIANEVLPEKQLQYIVSRD
                     AEAFYYSLKGLLDDQRGLEELQRMNYIAYRQQFVDDSLIGDKWALFFKDMCSKSQNEF
                     KNIFLREQKIPSFKERLISKAIYNLSRLALRLNLMNTLKQYSLLRKIYYKVAGTTTNQ
                     VDYDLLDEFYSNAISDKEVLAFYSAYWSGVATSTASFFKDDSIQYPYYSYEFPQVSNH
                     VYLNRLAEKLAESKTLKSVIMSGGTQLQMELAKKIKALSPKIKIFFAWHGSPAQWVDS
                     SQYSTFDGWYELYRERIADGVISFKPELDEILNAYGIRAFSVSNYAVSGSSFSKKLAA
                     PDSANFRVGLFAAMFSWYKNPFPQLLAIGSLKGCELVTNLHLEHNMKWVTSNIKLTTL
                     SENLNNKRFIELLSRLHVICYVTNTECSPMIALESASVGTPCIVGPAGNIYKGFPELE
                     LYLVEKEVDNPTAIRNRLQLVRDNYEEITVLLNAFVLEYNSRLELIKEKMYKELKQ"
     gene            5639..6418
                     /gene="wzm"
     CDS             5639..6418
                     /gene="wzm"
                     /note="O101_04"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="BAQ01470.1"
                     /db_xref="GI:733166929"
                     /translation="MKESLTDIYLSIKSWHLWTTLGWLELRQRYKRSIVGPFWITLSM
                     AVLIIALGVIYGELFKLEVKEYLPMLAIGLVFWSFISGVINEGCVTFISSSSFISQLP
                     TKKFIYIMQMLWRNFMMLCHNIIIVIGVLIIFNVFSLRNFPLFILGFMIVAVNLIWIA
                     TLFAMISVRFRDFPQMVMSFMQVVFYITPILFSGTMLAKYSLLLKLNPFAWFIEIIRS
                     PLVGKMPELYCYYNSIIFACVGTIISLITFAKCRNRIAYWV"
     gene            6425..7177
                     /gene="wzt"
     CDS             6425..7177
                     /gene="wzt"
                     /note="O101_05"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="BAQ01471.1"
                     /db_xref="GI:733166930"
                     /translation="MSHLIDLKDVGVEFPIYDAKSRSFKSQVANLTRGVISSKKESST
                     VTVKSLDGLTFSLKEGDKLGLIGNNGAGKSTLLKLLAGIYEPTSGTIKRKGKIVPLLD
                     IALGMDDDSTGYQNIKLRGLLLGMTNAEIESKIEEIACFTELGDYLNLPIRVYSTGMR
                     VRLAFAVSTSVDPDVLLLDEIIGTGDASFLNKAKKRFEELQERAKVVVLSSHDNNVIL
                     KTCNKVLWLEGGKPKMIGDPEEVLNKYMDSVI"
     CDS             7194..8273
                     /note="O101_06"
                     /gene="wekV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01472.1"
                     /db_xref="GI:733166931"
                     /translation="MKIDYVITGMNVGGAEMQVVELLLELKRCGHSVRLISLTPPVDL
                     IDRLIRNNIPVVSLGMKSKLGLPFAAFKLARLMRQNSPDIVHSHMFHANILVRLVRIF
                     LVDSTSIICTAHSIREGGSARDWLYRLTNKLSDLNTVVSDAARLRFINDKVFPSEKTL
                     TVYNCINIDHFRIIDRQSRKRFRWVTVGRLVKVKNQLLILQAMMKLPLSELIIIGEGE
                     ERLSLQTFIDQNSLSERVTLLGKKDNVVDYYHDSDAFVLASDYEGFALVVAEAMACGL
                     PVVATNCGGPAEIIGNGQDFGITISVNDVHHLTAAMQQIESLDVEQRRGSGYRARERV
                     IEKFSTKKIVSQWEDIYLRIQKNRI"
     CDS             8283..9437
                     /note="O101_07"
                     /gene="werS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01473.1"
                     /db_xref="GI:733166932"
                     /translation="MKIALIGTTAESLIQFRKELINLLHKNGHVVYAFAIDYDDVTKE
                     KVKSFGAIPVHYCFSRTGLNPFSDIINTYKLYKILKSLKLDLVFSYFSKPAIFGTLAA
                     KFARIQKRYAMLEGLGFFFTNSGGQVSLRNKLLKKILVSLYKLSFRHIESLILLNADD
                     KRDLLVNAKIQVKKVHILGGIGLNMSDYPYCPPPVDPVSFIFVARFLQEKGVYEFIEA
                     AQVVKKKFPETHFCMLGHLDLHNPGSLTTERLNDLKCNNIIELPGHVDNVQEWLAKAS
                     VFVLPSWREGFPRSTQEAMAMGRAVITSDVPGCRDTVVDGVNGFLIKPRSSHALAEKM
                     LCFLHQPELITQMGNASHQIALQQFDSYIVNSKLMKILRVDDENTKSNDC"
     CDS             9486..10634
                     /note="O101_08"
                     /gene="werW"
                     /codon_start=1
                     /transl_table=11
                     /product="putative methyltransferase"
                     /protein_id="BAQ01474.1"
                     /db_xref="GI:733166933"
                     /translation="MERLNLSEKPAHSLQEAAIHCARYANILHLVKDKVVLDIACGEG
                     YGSALLMKAGAKRVVGVDISQESIEHAKKLFGGYNVEFIVSDACTISERYGEDFFDMV
                     VSIETIEHINTPEVFLSAIKKTAKENAIFYITCPNDYWYYPNDEQSNPYHVRKYTFQE
                     FTELSTVILGKNVQWAYGCSAFGFCTVSANNRGFEKIGSSWMEISDSENSITVLNTDI
                     DAVSENNCSFFVGLWNAPETNFSNSTFPISMNAYSRMTEEFESNVSLVLREEQAENKK
                     AISILKQDMKKLTMDLRKSNLLLFALKIENDALRTNISELQQQLARKIAMYDEVTNTN
                     NNLVTTNSSLMERVSQMEIPYYRYVRLSALLPDFLKKMILKIMRLIRK"
     CDS             10637..12718
                     /note="O101_09"
                     /codon_start=1
                     /gene="werT"
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01475.1"
                     /db_xref="GI:733166934"
                     /translation="MSARLKLLPGVKSTEYVYSDLHALLDSNAESSFGPNIGSDGSNL
                     TITCLSMSRVFLTEKLVNSIYQHIPNFKGDILIVDNGSTLEELSVLQTLSDRIPLHIR
                     VIELGNNFGVSGGRNKALEHIKTEWAMFLDNDIYFINNPFPRLQKDISKLGCHFINMP
                     LLDSDGETLFANGGNIFLHIDNDSVHVGAGSACVQEKITTYDGEGFLSTFLFGGASVI
                     KIDSLKSLGKYDENMFIGFEDIDLSLRLYQSGMKVGTCGAISLIHDHPKPSSNKDKDY
                     EKIRFTKSILKESADYLENKWGMVFWSTPVDDWLEEKQRSFELITQKESNYSQIDDNK
                     QNVICDFPVAKPRIALIIDTENWAFSNIANQIVNHLSDSYDFTIIPTEIIDNISQVIM
                     MTKNHDITHFFWRESLRLIYDECYINYNRAIGFDELSFKKQYLDGRIITSSVYDHLFL
                     DEQAIKLRKTFYNDLITAYTVSSSKLYDIYSSIAEYPNPSVLAEDGVDLKLFYPLDLE
                     RFDNIESRTLRVGWAGNSKWAGEVEDFKGYHSLLKPAVEQLQSEGLNIELVLADRQLG
                     FIPHDEMVNYYSQIDVYVCPSKIEGTPNPVLESMACGVPVISTDVGVVNDAFGDMQKE
                     WILPVRSKDVLIDKLRDFYNRRNTVVKCLSSENLQQIKKWDWKVKSENFRIFFDKILE
                     SKKHSKINTEM"
     gene            12808..14214
                     /gene="gnd"
     CDS             12808..14214
                     /gene="gnd"
                     /note="O101_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01476.1"
                     /db_xref="GI:733166935"
                     /translation="MSKQQIGVIGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAVIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AIAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELADT
                     FTKWNEGELSSYLIDITKDIFTQKDEEGKFLIDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQLAGDKVEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYKWDLQYGEIAKIFRAGCIIRAQFLQKITDAYANNDKIENLLLDPY
                     FKNIADEYQQALRDVVTYAVQNGIPVPTFGAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLN"
     CDS             14467..15795
                     /note="O101_11"
                     /gene="IS4"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS4"
                     /protein_id="BAQ01477.1"
                     /db_xref="GI:733166936"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     CDS             15836..16909
                     /note="O101_12; oxidoreductase"
                     /gene="wbxC"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             16931..17941
                     /note="O101_13"
                     /gene="werU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01478.1"
                     /db_xref="GI:733166937"
                     /translation="MKSESFVSVVLSTHDLSSELTNKLKQIQSLLDDNYTDYEILIVS
                     KGPYRDVPRTSAIDNILKTIPSVRFLQLSGCVHDDVVRAAGLENAIGDFVVLYDHQTD
                     PVYVIHQAVEECKSGYDVVVGVSTQSLPCAYRIMRDTASHILKAIDYHIPKNSTGLRC
                     LSRRAVNAVTDTGRFHHQFYMRIQKTGYPVSELTYQPVSETHIKRSITYGFRNLVRLM
                     VFNSSRPLRWMSIVGTVGSLSALLFAVYSILVNLINGHVVEGWTTTVLFMSTLFMLQF
                     IMLAFFGEYLSRLLDDRSEQAAYSVVFEKNSAVMINKDRVNVLNDATVEEANLVQTGR
                     DR"
     CDS             17956..18738
                     /note="O101_14"
                     /gene="wbxD"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01479.1"
                     /db_xref="GI:733166938"
                     /translation="MTKALIGYTGFVGTTLLRQTKFDDFYRSTNINDIKGKKFDLVVC
                     AGAPAKKWIANADPKGDQETIDSLIECLNNIECKTLILISTVDVFKSPNGVDENSVID
                     TQGLHAYGLNRYRLERFVAKRFQQHLIVRLPGLVGPGLRKNIIFDFLNDNNVEKIESR
                     SIFQFYPMVNLWFDIKVALENKINLIHLTAAPVSVAEIALDGFGINFDMQQAAQPVFY
                     DMRSVYGALFGGSAHYQYSVKDTLQAVRHYAQSEPKKRAGIQ"
     CDS             18739..19551
                     /note="O101_15"
                     /gene="wbxE"
                     /codon_start=1
                     /transl_table=11
                     /product="putative xylose isomerase"
                     /protein_id="BAQ01480.1"
                     /db_xref="GI:733166939"
                     /translation="MRVSISNLAWEVTEDVEVANLLSAFNIDAIDIAPGKYFPEPTNA
                     TIADIQTVKEWWQERGIDIIGMQALLFGTTGLNVFGSKSVQKKLLNHLVAVCRIGAGL
                     GATRLVFGSPKNRDCSGLSVSQVQNISQKFFRQLGDIAADHGVIVCLEPNPSVYGANF
                     MTTSFETCRVVHEIDHPAIKMQLDTGAVIINQEDVSDVVTQAKDVIGHVHISEAQLAP
                     PGDSYFRHDDIAAVILSQLPAHTATIEMLATRNEPHLVSIRRALQVVTSLYR"
     CDS             19854..20738
                     /note="O101_16"
                     /gene="werV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01481.1"
                     /db_xref="GI:733166940"
                     /translation="MDDNCWRGVCHRRCCINYVKSCLIMDLSMKHEKNWEVPSYDVTL
                     WTEKKHDACVVIPVINEGSRIISLLTKMSNLKINNIADIIIVDGGSTDGSLEKDRLLS
                     LGVSGLVLKIGPGKLSAQLRCGYAFVLEQGYKNIITIDGNDKDDPSPIPEFLNALEKG
                     YDFVQASRFINGGVAVNTPKLRDFAIKYIHAPALSIASGFHWTDTTQGFRAYSSKLLL
                     DGRVAPFRNIFMTYELLAYLSYIAPKLGFRCIELPTSRIYPNGDVPTKISGFNGNLSV
                     LIILFKTCFGRYNSNRCN"
     CDS             20791..22161
                     /note="O101_17"
                     /gene="wexK"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01482.1"
                     /db_xref="GI:733166941"
                     /translation="MGVVMNIFGFDHERVKIEVVLLIICVGGMLCSIYAFYPGWMSPD
                     SIFQYNNAVTGYYNSWHPVIMAWWWRVLGYITDGPGPFLVQNLMLFWIGFYFIAKTLS
                     RYIGWGSIVVLLLAATPSVLIIMAQLWKDAVFTSLAIFGLGIIFRSIDTGRFYKSVFL
                     LFVFVLSLAVGCKPNGLFVAIILIGWWFIANPKVSTKRSKLILSLTSVFLVMAIPFSI
                     SKCLPVYKISPIQYIQSYDLLGISVIENKTLLPDYITKKVGLSKDNAREFYFPGGNNL
                     MFYNSPAGNIATTDPEYLKDLQSRWISAIKDYPMAYIKVRFDNFIELLRWGAIHPASV
                     AADVIVNNPWGYVFIPNKLSDNYIDSIDKYPFLYFPWIYLVTSALLFLLTKVLPRELR
                     FFNVVLVLVVFSFSLPHFFIAPAADYRYLHFSVVCAVVQLGMFLGFFFMRLYKRVYFG
                     AIKLLG"
     gene            22484..23650
                     /gene="ugd"
     CDS             22484..23650
                     /gene="ugd"
                     /note="O101_18"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01483.1"
                     /db_xref="GI:733166942"
                     /translation="MKITISGTGYVGLSNGILIAQSHEVVALDIIQTKIEMLNQKQSP
                     IVDKEIEEYLATRSLNFRATTDKCDAYLDADYVIIATPTDYDPKTNYFNTSSVESVIR
                     DVVAINPDAVMVIKSTIPVGFTNSIKEKLGTENIFFSPEFLREGKALYDNLYPSRIVI
                     GERSERAERFAALLQEGALKKDIPTLFTDSTEAEAIKLFANTYLALRIAYFNELDSYA
                     ETLGLNSLQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLSNYESVPNNLIGAI
                     VEANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGITVII
                     YEPVMQEDEFFNSRVVRDFEAFKLEADVIISNRMAEELVDVGDKVYTRDLFGND"
     CDS             23738..24241
                     /note="O101_19; transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             24497..25132
                     /note="O101_20; transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     gene            complement(25468..26079)
                     /gene="hisI"
     CDS             complement(25468..26079)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01484.1"
                     /db_xref="GI:733166943"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQNLDLTTVIENLRKRHQ"
BASE COUNT     7711 a   4846 c   5953 g   7897 t
ORIGIN
        1 tcggtgccaa catcaccaac tgtaagttca gcgacttaca aggcgacgcc attgaatgga
       61 acgtggcgat taacgacagt gatattttga tatctgacca tgtcatcgag cgcatcaact
      121 gtaccaacgg caaaatcaac tggggcattg gcataggtct tgcgggaagc acttatgata
      181 acaactaccc ggaagaccag gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg
      241 attgtcggca gttgatccat gttgaaaatg gtaaacattt tgttatccgt aatatcaaag
      301 cccgcaatat cacgccggat ttcagtaaga aagcaggcat tgataacgcg acagttgcta
      361 tttacggttg tgacaatttc gtgattgata atattgaaat gattaatagc gccgggatgt
      421 taatcggcta tggggtaatt aaaggcaaat atctctcgat accgcaaaat ttccgagtga
      481 ataatattca actggataac acccatcttg cttataaatt gcgcggcatc caaatctccg
      541 ccgggaatgc tgtctccttt gtggcactga ctaacattga gatgaagcgt gcgtcgctgg
      601 agttacacaa caaaccgcaa catcttttta tgcgtaatat caatgtgatg caggaatcct
      661 cagttggatc agcattgagc atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta
      721 tggcgaaaaa agaaacactg ctgtcacttg caaatgttca tgcggtgaat gaaagagggc
      781 aaagctccgt cgatatcgac aggattaatc accatattgt taatgtggaa aagattaact
      841 ttagattgcc gcaacggaga gagtagattt gcgaccattc ctggaaaaat ggagccatac
      901 ttaggaacaa tgctactgca atccacaacg aagcggcgta acatcacaag taattcagta
      961 atcaattcag gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat
     1021 tttacaggtg gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtcttgg
     1081 gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa
     1141 accaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
     1201 ggtaactcac gcgtccaaga atgcggtcga aaaccacttc gacacctctt atgaattaga
     1261 atctctcctt gagcagcgcg tgaagcgtca actgcttgcg gaagtgcagt ccatctgtcc
     1321 accgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttgg gccactccat
     1381 tttatgtgca cgacctgcta ttggtgacaa cccgtttgtc gtggtgctgc cggacgttat
     1441 tatcgatgac gccagtgccg atccgctgcg ttacaacctg gctgcaatga ttgcgcgctt
     1501 taacgaaacg ggccgcagcc aggtcctggc aaaacgtatg ccgggcgatc tctctgaata
     1561 ctccgtcatc cagactaaag aaccgctgga tcgtgaaggc aaagtcagcc gcattgttga
     1621 atttatcgaa aaaccggatc agccgcagac cctggactca gacatcatgg ccgttggtcg
     1681 ctatgtcctt tctgccgata tttggtcgga gctggaacgt actcaacccg gtgcatgggg
     1741 acgtatacaa ctgactgatg ccattgcaga actggcgaaa aaacagtccg ttgatgcaat
     1801 gctgatgacc ggcgacagct atgactgcgg taaaaaaatg ggctatatgc aggcgtttgt
     1861 gaagtatggg ctacgtaacc tgaaggaagg ggcgaagttc cgcaaaggta ttgagaagtt
     1921 gctcgggaaa taatcaagac aacagtttat ttgaattggc aatacttcat attaaatggc
     1981 aggagaagtc tcgtggtgct atcggtaggc agcatgactc tactgccttt tttgtgataa
     2041 aaatcaagca gtaaaaaacc aatattctac cttttcttgt agcaaaaatc gatttttcct
     2101 tgtttcttgg tgagattaat acgacaatat ctggaatgtt ctggggctaa atgtgtcaaa
     2161 atgtaatcga taattggtag attgagcccc gcaaaaattg gagtgtatag tatgctggta
     2221 gctgttgagc caggggcggt agcgtgcctg aataaattaa catttcggtg ttaaaataag
     2281 gtaacgttat gttaaaggct aacttacaac aaatataaga ccaaataatt gaatacgtaa
     2341 ttagcataaa agattaatac aatttcggag acgtcatgac tatacttgta acaggcggcg
     2401 ctggttatat tggttcacat accgttctac aattaattga gggcgggtat gatgttgttg
     2461 tgcttgataa cctgagtaat tcatcccaag agtcactaca tcgcgttggg tctttggttt
     2521 cgaaaaatat tccattcatt aaggctgata tcaacgataa acaaaagctt gaagaagttt
     2581 ttaaaaacta cgatattgat gcagtgattc attttgcagg tttaaaatct gttggtgaat
     2641 cagtaaagaa accactggaa tattacatta ataatgtcag tggaaccctt accctgcttg
     2701 aagttatgaa gcaagcaaat gtttacaaat tcatttttag ttcgtctgcg actgtttatg
     2761 gtaatcattc tccggttccc aatctggagg aatatcctat tggaaatgct tcatgcccct
     2821 atggaactac taaagtcatg ctggagcagg ttttatcaga ctatgcgaag tcagatgata
     2881 aattaaaaat tattgctctg cgttatttta atcctacagg tgcgcatcct agtggtctta
     2941 ttggggagga cccgaacggc attccaaaca atcttgttcc ttatatttct caggttgcta
     3001 ttggcaaatt agaaaaactg tccgtttatg gtggcgatta tgaaactatt gatggaacag
     3061 gagtacgcga ctttatccat gtggtagatc ttgccgcagg ccatgttagt gctttaaggt
     3121 atattgataa attacaagga tatgacattt ttaatcttgg tactggcact ggtgtatcag
     3181 ttttacaagt aatcgatgcc tttgaaaaag caagtaatca gaaagttaat tatgaaattg
     3241 tcgccagacg tgagggcgat ataggatctt cttgggctga tgtatctaaa gcagaaagga
     3301 ttttaggctg gaaatccaag tacaatattg aagatatgat ggtccatacc tggaattggc
     3361 aaaaacaaaa ccctaatggc tatggaagtt agtacctatt gatttaatat tataatggcg
     3421 tatcaatgaa tttatttttc atagtcgata agttggattg ggcattcggt agaatagctc
     3481 aaaatatagc aacagaatta agtagcttta atgtaaaaat agtttgcacc gatgatttta
     3541 aaactgtggc agactttgct atgcacatgg ataaaaacgt aaaagaaaag agtatattac
     3601 acttcttttg gcgtgattat cttatggaag tgatgaactt cactcagcgg aatccccaat
     3661 ataaagaaat tttgatcaga aatattatca caacgcatat tcctgatcat ctttttactt
     3721 atgatgatat cgcaaactat gagagaagaa ttcctcttat gaattttgtg gatgggtatt
     3781 ttgttacatc cggaaaattg aaagagctat attctgaact gtctgatttt ccgcctcctc
     3841 atagcgttat ctatgataat ccagaaattc ctatgagcga ggtaaaaagc tatgataatc
     3901 atgatagaaa aaaaatcgta tggattggca atagtaaatg gggagaatac ctcggttacc
     3961 aagattataa aggcctgaat acaatagttt tgccggctct agataaatta aaaaaatctg
     4021 gatatacatt tgattatatt gagtttgata gttctaaggg caagaccgat cgtgaggtaa
     4081 ttcttaaaac actttctgat gcagatattc tactgatcag ttcgaaatgt gaaggtacac
     4141 cattgccact tcttgaagca atggcaaaca gttgtgctgt tgtgtcgaca gatgttggta
     4201 tagcaaatga agtattacct gaaaagcagt tacagtatat tgtgtctcgg gatgcagagg
     4261 cattttatta ttcattgaaa ggtcttcttg acgatcaacg cggtcttgaa gagttacaaa
     4321 gaatgaatta tatagcgtat cgtcagcaat ttgtagatga tagccttata ggtgacaaat
     4381 gggcattgtt cttcaaagat atgtgttcaa aatcgcaaaa tgaatttaag aatatattcc
     4441 ttcgcgagca gaaaattcca tcatttaaag aacgattgat atcaaaagct atttataatc
     4501 taagcagact agctcttcgt ttgaatttga tgaatacgct aaaacaatat agcctgctac
     4561 gaaaaatata ttataaggtt gcaggaacaa ctacaaatca agttgattat gatttgttgg
     4621 atgaatttta tagtaatgca atatcagata aagaagtatt agcattttac tctgcatatt
     4681 ggtctggtgt tgccacatca acagctagtt tctttaaaga tgatagtatc caatatccat
     4741 attattcata tgagttccca caggtttcta atcatgtcta tctaaacaga ttagcagaaa
     4801 aacttgctga atctaaaaca ctaaaaagtg tgatcatgag cggtggaaca caattacaaa
     4861 tggaacttgc caaaaaaatt aaggcattaa gtccgaaaat aaaaatattt tttgcatggc
     4921 atggttcacc tgctcagtgg gttgactcct cgcaatattc aacgtttgat ggatggtatg
     4981 aactttaccg agagcgaatt gctgatgggg tcatctcgtt taagcctgag ctagatgaaa
     5041 tactcaatgc ttacggtatt cgtgcattct cagtatcgaa ttatgctgta tcaggctcct
     5101 cgttttctaa aaaattagct gcacctgatt ctgccaattt ccgcgtaggt ttgttcgctg
     5161 caatgttcag ttggtataaa aatccatttc ctcaattact agcaattggt tcattaaaag
     5221 gatgtgagct agttacaaac ttgcatcttg aacataatat gaagtgggtt acttcaaata
     5281 tcaagcttac gacattatca gaaaatttaa ataataagcg gtttatcgaa ctgctatctc
     5341 gtttacatgt tatatgctat gtaacgaata cggaatgttc gccaatgata gcgttggaat
     5401 ctgcaagtgt cggaactcca tgcattgtgg ggccagctgg aaacatatat aaaggttttc
     5461 ctgagttaga attgtatttg gttgaaaaag aggttgataa tccaactgca atcaggaatc
     5521 ggttacaatt agttcgtgat aattacgagg aaattacagt tttgcttaat gcatttgttt
     5581 tggaatacaa ttcccggcta gaattaatta aagaaaagat gtataaagag ttgaagcaat
     5641 gaaagaatca ctaacagata tatatttgtc aataaaatcg tggcatcttt ggaccacatt
     5701 aggttggtta gagttaagac agagatataa aagatccatt gttggaccat tttggattac
     5761 actaagtatg gcggtcctta ttattgcctt aggggtgatt tatggagaac tatttaaact
     5821 agaagttaaa gaatatcttc cgatgcttgc aattggtttg gttttctgga gctttattag
     5881 tggcgttata aatgaaggat gtgttacttt catatcgtcc agctccttta taagccaatt
     5941 gccaactaaa aaattcatat atattatgca aatgctatgg cgaaatttta tgatgctttg
     6001 ccataatata ataatagtga ttggtgtgtt gattatattc aacgtttttt cgttgaggaa
     6061 tttccctctt tttatacttg gctttatgat cgtagctgtt aacttgatct ggattgcaac
     6121 tttgttcgcg atgatttctg tacgttttag agattttccg caaatggtca tgagctttat
     6181 gcaggttgtc ttttacataa ctccaatatt attttctgga acaatgctgg ctaaatatag
     6241 tttactgctg aagttaaatc catttgcttg gtttattgag attattcgat cgcctttagt
     6301 gggcaaaatg ccagagctat actgctatta taattccatt atctttgcat gcgtaggaac
     6361 aattatctct ctaattacat ttgcaaaatg tagaaaccgc attgcatatt gggtgtaaat
     6421 tattatgtct catttgattg acttgaaaga tgtgggtgtt gaatttccca tttacgacgc
     6481 taaaagtaga tcgtttaaat cgcaggtggc aaatttaacc cgtggtgtaa tttcttcaaa
     6541 aaaagaaagc agcacagtta cggttaaatc attggacggt ttgacattta gtctgaaaga
     6601 aggtgataaa ctcggactta ttggaaataa tggtgcggga aaatctacgc tactgaaact
     6661 cttggctggt atttatgaac caacatccgg cacaattaaa cgaaagggta agatagttcc
     6721 tctgttggac atcgctctag ggatggatga tgattccaca ggataccaaa atataaagct
     6781 acgtggttta ttactaggta tgacgaatgc agaaatcgag tccaaaatag aagagatcgc
     6841 ttgttttact gaactaggtg attatctgaa tctaccaatt cgtgtctact ctaccggcat
     6901 gcgcgtgaga ttagcattcg cagtatcaac ttcagttgat ccagatgtac ttctactcga
     6961 cgaaattatt ggtactggcg atgcttcctt cttaaataaa gcaaagaaaa gattcgagga
     7021 attgcaggag agggcaaagg tagttgtgtt gtcttcgcat gacaataatg tcattctgaa
     7081 aacttgtaat aaagtcctgt ggttagaggg cggtaaacca aagatgattg gagatccgga
     7141 agaagtacta aataaatata tggatagtgt gatttaaaaa acaggtggta tgagtgaaaa
     7201 ttgattatgt cattacaggc atgaatgtcg gaggcgcgga aatgcaggta gtagaactgc
     7261 ttctcgagct taaacggtgt gggcattctg ttcggttaat ttcgcttaca ccaccagttg
     7321 atttgatcga taggctaatt agaaataaca tccctgttgt ttcattgggg atgaagtcca
     7381 aattaggttt accatttgct gcatttaaat tagccaggtt gatgagacag aattctccag
     7441 atattgtgca ttcgcatatg tttcatgcca atatcctagt acgtcttgta agaatatttt
     7501 tggtggatag tacatctatt atttgtactg cgcacagcat tcgtgaaggt ggcagtgcta
     7561 gagactggct ttatcgtttg actaataaat taagtgactt gaatacagtc gtaagcgatg
     7621 cggcacggct gcggtttatt aacgacaaag tatttccatc agagaaaaca cttactgttt
     7681 ataattgtat caatattgat cattttcgta tcattgatag acaaagcagg aaacgatttc
     7741 gctgggtaac cgttgggcgg cttgtaaaag taaaaaatca acttttgatt ttgcaagcga
     7801 tgatgaagtt gccacttagt gaactcatca taattggaga aggcgaggaa cgactctcat
     7861 tacaaacatt tatcgatcaa aattctctaa gcgagcgagt tactctcttg ggtaaaaaag
     7921 ataatgttgt tgattattat catgattctg atgcttttgt gctggcctca gactatgaag
     7981 gatttgcact cgtcgtggcc gaagctatgg catgcggatt gccagttgtc gcaacaaact
     8041 gtggtgggcc tgctgaaatt attggaaatg gacaggattt tggcataaca atttcggtta
     8101 atgatgtaca tcacttaacc gctgcgatgc aacaaattga atcattagat gttgaacagc
     8161 gtagaggaag tggctatcgt gctcgggaaa gagtcattga gaaattttct actaaaaaaa
     8221 tcgtatctca atgggaagat atctacctgc gcatacagaa aaacagaatt tagaattcac
     8281 ctatgaaaat agcattaatc ggtactacag cagaaagtct aatccaattc agaaaagaac
     8341 tgattaattt attacataaa aatggccatg ttgtgtatgc gtttgcaatt gattatgatg
     8401 atgtaacaaa agaaaaagtt aaatcatttg gtgctatacc agtacattac tgtttcagtc
     8461 gaacagggct aaatccattt tctgatatta tcaataccta taaattatat aagattctaa
     8521 aatcacttaa gttggacttg gtattttcat atttttcaaa gccagccatt tttggtactt
     8581 tagcagcaaa atttgcaaga atacagaagc gttatgcgat gcttgaaggg cttggttttt
     8641 tctttaccaa ttctggaggg caggtttcat tacggaataa attactaaag aaaatattag
     8701 tatcacttta caaattaagt ttcagacata ttgaaagtct tatcttgctt aatgcggatg
     8761 ataagcgaga ccttttagtt aatgcaaaaa ttcaagtcaa aaaggttcat atattagggg
     8821 ggattggatt aaatatgtct gattacccct actgcccacc gcctgttgat ccagtgtctt
     8881 tcatttttgt cgctagattt ttacaagaaa agggagttta tgaatttatt gaggctgcac
     8941 aagtagtcaa aaagaaattt cctgaaactc atttttgcat gctgggtcat ctggatctcc
     9001 ataatccggg gagcttaact acagagcggc ttaatgattt gaagtgtaat aacatcattg
     9061 agttacctgg ccacgttgat aacgtgcagg agtggcttgc taaagcgagt gtatttgtat
     9121 tgccttcctg gcgtgaaggg tttccgagga gtacacagga ggcgatggca atgggacgtg
     9181 ctgtcataac cagcgatgtg ccaggctgcc gagatacagt agttgatggt gttaatggat
     9241 ttttgattaa gccacgttct tctcatgcgt tagcggaaaa aatgctctgt tttttacatc
     9301 aacctgagct aattacccaa atggggaatg caagtcacca aatagcttta caacaatttg
     9361 actcatatat agttaattca aaacttatga aaatactgcg cgtagatgat gaaaatacaa
     9421 agtctaatga ctgttaaatc atataaataa aacgttataa taaatacaat ttagggggga
     9481 atattatgga aagattaaat ttgagtgaaa aacctgctca ctctttacaa gaagcggcta
     9541 ttcattgtgc cagatatgct aatatcttac atcttgttaa agataaagta gttctcgaca
     9601 ttgcctgcgg cgaaggatat ggctcagcac tattaatgaa agctggtgct aaacgagttg
     9661 tgggggtgga catctctcaa gagtcaattg agcacgcaaa gaaacttttt gggggatata
     9721 atgttgagtt tattgtaagt gatgcatgta ccatctcaga gcgttatggt gaagattttt
     9781 ttgatatggt tgtttcaata gagacaatag aacacatcaa tactcctgaa gtttttctta
     9841 gtgctatcaa aaaaactgct aaagaaaatg cgattttcta tataacttgc cctaatgatt
     9901 attggtatta tccaaatgat gaacaatcca atccgtacca tgtacgaaaa tatacttttc
     9961 aagagttcac cgaacttagt acagtgattt tgggaaagaa tgttcaatgg gcatatggtt
    10021 gttctgcttt tggattttgt acagtctcag caaataatcg aggttttgaa aaaataggtt
    10081 cttcatggat ggaaataagc gattcggaaa attcaattac tgttttaaat acagatattg
    10141 acgcagttag tgaaaataat tgcagttttt ttgtgggctt atggaatgca cctgaaacaa
    10201 attttagcaa tagtacattc ccaataagta tgaatgctta ttcgcgcatg acagaagagt
    10261 tcgagtcgaa tgtaagtctg gtacttcgtg aggaacaggc agaaaataaa aaagctatat
    10321 caatattaaa acaagatatg aaaaagttaa caatggacct gagaaaatca aaccttttat
    10381 tattcgcatt aaaaatcgaa aatgatgcat tgcgtactaa tatttccgaa ttgcaacaac
    10441 agcttgccag gaaaattgcg atgtatgatg aagtaacaaa tacaaataat aatttagtaa
    10501 ctacaaattc atcattgatg gaaagagtgt cacagatgga aattccatat tatcgatacg
    10561 taaggctatc cgcccttctt ccggattttt tgaaaaaaat gatacttaaa attatgcgtt
    10621 tgatcaggaa ataaaaatgt cggcacgttt aaaattatta ccaggggtaa aatcaactga
    10681 atatgtgtat tcagaccttc atgctttgct cgattctaat gctgaaagct cgtttggtcc
    10741 gaacattggt agtgatggtt ctaacctaac aataacatgt ttatcgatga gtagagtttt
    10801 tcttactgaa aaacttgtta attctatata tcagcatata cctaatttca aaggtgatat
    10861 cctgattgtt gataacggca gtacattaga agaactttca gttttacaaa ctttaagtga
    10921 taggattcca ttacatatta gggttatcga acttggtaat aattttggcg taagtggcgg
    10981 aagaaataaa gcgttagagc atataaaaac agaatgggct atgtttcttg ataatgatat
    11041 ttattttata aacaacccgt tcccgagatt gcaaaaggat atttcaaaac ttggctgtca
    11101 tttcatcaat atgccattgc ttgattctga tggagaaaca ttattcgcaa atggagggaa
    11161 tattttcctc catattgata atgattcggt gcatgttggt gcaggaagtg catgcgtcca
    11221 ggaaaaaata actacatatg atggtgaggg cttcctttct acatttctct ttggcggtgc
    11281 gagtgttatt aaaattgact ctctgaaaag tttagggaag tacgatgaaa atatgttcat
    11341 tggatttgag gatattgatt tatcgcttcg tctttaccag tcaggaatga aagtaggtac
    11401 atgtggtgca atatctttaa tccatgatca ccccaaacct agttctaata aagataagga
    11461 ttatgagaaa attcgcttca cgaaaagtat attaaaagag tctgcggatt atcttgaaaa
    11521 caaatgggga atggttttct ggtcaactcc agttgatgat tggttggaag agaagcaacg
    11581 tagttttgaa ctgataactc aaaaagaaag taactattcg caaattgatg ataataaaca
    11641 aaatgtcatt tgtgattttc cagtagcaaa accgagaatt gcattgatta ttgatactga
    11701 aaactgggca ttctcaaata tagcaaatca aatagtcaat catctgtcag attcgtatga
    11761 ttttacaata ataccaactg aaatcattga taatatcagt caggttatta tgatgaccaa
    11821 aaatcatgat ataactcatt tcttctggcg tgaatcgctt agacttatat atgatgaatg
    11881 ctatataaac tataaccggg ctattggatt tgatgagctt agttttaaaa aacaatactt
    11941 agatggaagg ataataacaa gtagtgttta tgatcatcta tttcttgatg aacaagctat
    12001 aaagttaaga aaaacatttt ataatgattt aataactgct tatacagtta gttcatcaaa
    12061 attatatgat atatactcat caatagccga gtatcctaat ccgtcagttc ttgctgaaga
    12121 tggtgtcgat ttaaaactat tttatcctct cgatcttgaa cgctttgaca atattgagtc
    12181 gagaacattg cgcgtaggtt gggctggtaa ttctaaatgg gcgggagaag tagaggattt
    12241 taaaggttac cattctttat taaagccagc agttgagcaa ttacaatcag aaggattgaa
    12301 tatagagtta gtccttgctg atcgtcagct cggatttatt cctcatgatg aaatggtaaa
    12361 ttattatagc caaattgatg tctatgtctg tccttcaaaa attgagggca caccaaaccc
    12421 tgtactcgaa tctatggcat gtggcgttcc cgtgataagt actgatgtcg gtgtggttaa
    12481 tgatgcgttt ggtgatatgc aaaaagaatg gattttgcca gttagaagca aggatgtttt
    12541 gatagataaa cttcgggatt tttataatag gcgtaacaca gtggtaaaat gtttatcttc
    12601 agaaaatctg cagcaaataa aaaaatggga ttggaaagtt aaatctgaga acttccgcat
    12661 ttttttcgac aaaatattag aatctaaaaa gcacagcaag ataaacactg aaatgtgatc
    12721 aaaatatcat tttaacctta acacaggttt tttatcatta tcgtgagtta ataagatcgt
    12781 agtatctaca cctgacagga gtatgtaatg tctaagcaac agatcggcgt catcggtatg
    12841 gcagtaatgg ggcgcaactt ggcgctcaat attgaaagcc gtggttatac cgtttccgta
    12901 ttcaaccgct cccgtgaaaa gaccgaagaa gtcattgctg agaatccgga taagaaactg
    12961 gttccttatt atacggtaaa ggagtttgtt gaatcacttg aaactcctcg tcgtatcctg
    13021 ttaatggtga aggcgggcgc gggtacagat gctgtcattg attcacttaa gccgtatctg
    13081 gaaaaaggtg acatcatcat tgatggcggt aatactttct tccaagatac tattcgccgt
    13141 aaccgggaac tatctgccga aggttttaat ttcatcggca ccggtgtgtc cggtggtgaa
    13201 gaaggggcgt tgaaaggccc atctattatg cctggaggtc agaaagaagc gtacgagtta
    13261 gttgctccaa tattgactaa aatcgcggcc attgctgaag atggcgaacc gtgcgtaact
    13321 tatatcggcg ctgatggtgc aggccattac gtgaaaatgg tccacaacgg cattgagtac
    13381 ggcgatatgc agttgattgc tgaagcctat tctctgctaa aaggtggtct taacctgtca
    13441 aacgaagagc tggcagacac ttttaccaag tggaatgaag gtgagctcag cagttacctg
    13501 atcgacatta ccaaagacat tttcacccaa aaagatgaag agggtaaatt cctgatcgat
    13561 gtgatcttgg atgaagcggc taacaaaggc actggtaaat ggaccagtca gagttctctg
    13621 gacttgggcg aaccgctatc tctgattacc gaatctgtat tcgctcggta tatctcctcc
    13681 ctgaaagacc aacgcgtagc agcttctaag gtcttgaccg gacctaaagc tcagcttgca
    13741 ggtgacaaag tagagtttat tgagaaagta cgtcgtgcat tatatctggg gaaaattgtg
    13801 tcttatgcac aaggattctc acaattgcgt gctgcatctg atgaatataa gtgggatctg
    13861 caatacggtg agatagcgaa gattttccgt gctggctgta tcattcgtgc acagttcttg
    13921 cagaaaatca ctgatgctta tgctaacaat gataaaatcg agaacctgct cctggatccc
    13981 tacttcaaga atattgctga tgaataccag caagctctgc gtgatgtggt aacctatgcg
    14041 gtacaaaatg gtattcctgt accgacgttt ggcgcagctg tcgcttatta tgacagctac
    14101 cgtgcagcgg tactaccggc gaacctaata caggcgcagc gcgattattt tggtgcacat
    14161 acctataagc gtactgataa agaaggcgta ttccatacgg aatggttgaa ttgaataata
    14221 aattttcata ttgtctattt taataacaag gccatcttac agggtggcct tattgtcagc
    14281 aaaatttctt gctgtattaa atatttatat tattctatat ataaatattc ctcattaaag
    14341 gcattggttc gaacatgcga aaaaatataa taattgtagg tgtaatgccg atcagttaag
    14401 gatcagttga ccgatccagt ggctgtgtaa gaatccggaa acgctcactc gtttccggat
    14461 ttttttatgc acattggaca ggcccttgat ttggtatccc gttacgattc tctgcgtaac
    14521 ccactgactt ctctggggga ttacctcgac cccgaactca tctctcgttg ccttgccgaa
    14581 tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt ctggtgtatt
    14641 gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg cctggacatc
    14701 atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc ccgccagcgc
    14761 ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg gcataacgcc
    14821 acgccgcatc cgcaatggtg cggcctgacc ctgctggcca tcgatggtgt gttctggcgc
    14881 acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc cgggaacccg
    14941 gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca tctgctgacg
    15001 gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca acttatagaa
    15061 caaaccggcg ataacactct gacgttaatg gacaaaggtt attactcact gggactgtta
    15121 aatgcctgga gcctggcggg agaacaccgc cactggatga tccctctcag aaagggagcg
    15181 caatatgaag agatcagaaa actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc
    15241 ccgcaggcac gaaaaaagtg gccgggactg ggaaatgagg tgacagcccg cctgctgacc
    15301 gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc catgcgcttc
    15361 cccggaggag aaatggcgga tctgtacagt catcgctggg aaatcgaact gggatacagg
    15421 gagataaaac agacgatgca actgagcagg ctgacgctga gaagtaaaaa gccggagctt
    15481 gtggagcaag agctgtgggg tgtcttactg gcttataatc tggtgagata tcagatgatt
    15541 aaaatggcgg aacatctgaa aggttactgg ccgaatcaac tgagtttctc agaatcatgc
    15601 ggaatggtga tgagaatgct gatgacattg cagggcgctt caccgggacg tataccggag
    15661 ctgatgcacg atcttgcaag tatgggacaa cttgtgaaat taccgacaag aagggaaagg
    15721 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
    15781 cagtcagttg cttaactgac tggcattaat aattgtaggt ggtggttttt ttggaatgta
    15841 cctcgctgag caattttctc ttttaggtca tggagtaaag ctattcgaaa aggagaataa
    15901 ttatatgtct cgagcgtcat ataacaacca ggcgcgtgtg cacaatggct accattatcc
    15961 aagaagtatt ttgacggctt tacgttcacg tatctctttc ccgcgttttg ttgatgaatt
    16021 tcgtgattgc gttgattcag actttgataa gtattattta attagcgaaa tactggggaa
    16081 agtttcggcc agccaattcc gcatgttctg tgaccgaatt ggtgcagact gtgattatgc
    16141 tccagcgaaa atacagaaca tggttaatcc aaaaatgatt aatgcatgtt ttacggttaa
    16201 agagtttgcc tttgatgcct ttaagttgcg cgatataatg ctaaagagac tggacagttc
    16261 aaatgccgaa ttctattgtg gctattcggt tgataaaatc gagcaactgg aaaatggtat
    16321 taagacattg atcagagagg taagcacagg tgaagtgcag gagtatgttg ctgaccacgt
    16381 gtttaattgt acttattcac atctgaacta tcttctggat aactctggta ttgaactgat
    16441 ccctctgaag catgaaatga ccgaaatgtg tctggtagac gtgcctgaag tatgtaagaa
    16501 tgcaggtttt accataatgt gtggtccatt tttctccgtg atgccgttcc cttcagttgg
    16561 attacattca tttagtcatg tgcgttatac gccgcattat gagtggtacg ataaacctgg
    16621 tgaaaaatat ctcgattcac atgataaatt tgattcagat acaaaaaata gcgcttggca
    16681 atacatgatt agagatgcat cacgttatat accagtttta aacgaatgcc gttatgagcg
    16741 ttccctatgg gaagtgaaga cccttcttcc acgcagtgaa acagatgact cccgcccaat
    16801 cctctttagg cctaatcatt gtatacaggg attccactgt attatgggtg gtaaaataga
    16861 taacgtatat gatgcttttg aagccataaa aagtatgaac gttatttaaa atactataga
    16921 gactaaacaa gtgaaaagtg aatcttttgt ttcggttgtt ttaagtacgc acgatttatc
    16981 gagtgaatta acaaataaat taaagcagat tcagtcttta ctggacgata attacactga
    17041 ttatgaaatt ttaattgttt ctaaaggacc gtatcgtgat gtaccgagaa catctgcgat
    17101 cgacaatata ttaaaaacca ttccgagcgt gcgctttctg caactttcgg gctgcgttca
    17161 tgatgatgtg gtgcgggctg ctggtcttga gaatgcaatt ggtgattttg tggttttata
    17221 tgatcatcag actgatcctg tttatgtcat tcatcaagca gtggaagagt gtaagtccgg
    17281 gtacgatgtt gttgtggggg tctcaacgca gtcgcttccg tgtgcgtatc gtattatgcg
    17341 ggatacggcg tcgcatatat taaaagcgat cgattaccat attcctaaaa actctacagg
    17401 tttgcgctgt ctcagtcgtc gtgcggtaaa tgcagtaaca gatactgggc gtttccacca
    17461 ccagttctat atgcgcattc aaaaaacggg ctatcccgtc agcgaactga cttatcagcc
    17521 cgttagcgaa acgcatatta aacgttctat cacatatggt tttcgcaatt tagttcgttt
    17581 gatggtcttc aactcttctc gtcctttgcg ctggatgtcg attgttggca ctgtaggtag
    17641 tttgagcgct ctgcttttcg cggtatacag tattctcgtg aacctgatta atggccatgt
    17701 tgtagaaggt tggactacta cagtcttgtt tatgtccact ttgtttatgc tgcaatttat
    17761 tatgttggcc ttctttgggg aatatctcag tcgtttgctc gatgaccgaa gcgaacaggc
    17821 agcatattcc gttgtgtttg agaaaaacag tgcagtaatg attaacaaag accgcgttaa
    17881 tgtgttgaat gatgcgactg tggaagaggc taatctggtg caaaccggac gtgaccgtta
    17941 aatacaggag agatcatgac taaagctttg ataggatata ccgggtttgt tggcacaacg
    18001 ctcttaaggc aaaccaaatt tgatgatttt tatcgttcta caaatattaa tgatattaag
    18061 ggtaaaaaat ttgatctggt ggtttgtgcc ggtgcgccag cgaaaaaatg gatcgcgaat
    18121 gccgatccca aaggcgatca ggaaaccatt gattcgctga ttgaatgctt aaataacatt
    18181 gagtgtaaaa cgttgatcct catcagcact gtggatgtgt ttaaatcgcc caatggtgtt
    18241 gatgagaatt ccgtgatcga tacgcagggg ctgcatgcat atggtttgaa tcgttatcgc
    18301 cttgagcggt ttgtcgcgaa gcgattccaa caacatctga tcgtcaggct tccaggtttg
    18361 gtcggtccag ggctacgtaa gaatattatt tttgattttc ttaatgacaa taatgtagag
    18421 aaaatcgaaa gccgcagtat tttccagttt tatccgatgg ttaacctctg gttcgatatt
    18481 aaagtcgcac ttgagaacaa aattaatctt attcatctga cagcagcccc agtttcggtg
    18541 gcggaaattg ctctcgatgg tttcggtatt aattttgata tgcagcaggc ggcacaacct
    18601 gttttttacg atatgcgtag tgtgtatggt gcattgtttg gcggcagcgc gcattaccaa
    18661 tattcggtaa aagatacgtt gcaggctgtc aggcattatg cgcagtcaga acccaaaaaa
    18721 agagcaggaa tacaataaat gagggtatca atctccaacc ttgcctggga agttaccgaa
    18781 gatgtagaag ttgctaatct attgtctgca tttaacattg acgcgattga tatcgctccc
    18841 ggaaagtatt ttccggagcc tactaacgcg actatagcag atattcaaac ggttaaagag
    18901 tggtggcagg agagaggcat tgacatcatt ggcatgcagg cacttttatt tggaactaca
    18961 ggtctgaatg tatttggtag taaatctgtt cagaaaaaac tactcaatca cttggttgca
    19021 gtttgccgta taggtgcagg gctgggggct acgcgcttgg tatttggttc gcccaaaaat
    19081 cgggactgct ccggtctatc agttagtcag gttcagaaca tatcacagaa atttttccgt
    19141 cagttgggcg atatcgccgc tgaccatggt gttattgttt gccttgagcc aaacccaagt
    19201 gtctatggtg caaactttat gacaaccagc tttgaaacct gtcgtgtggt tcatgagatt
    19261 gatcatccag ctatcaaaat gcagcttgat accggagcgg ttattatcaa tcaggaagat
    19321 gtttctgatg ttgtgacaca agcaaaagat gtgattgggc atgtgcacat cagtgaagcg
    19381 cagcttgcgc cgccagggga ctcatatttt cgacatgatg atattgcagc ggtaatttta
    19441 tcgcaattac ctgcgcatac ggctactatt gaaatgctgg caaccagaaa tgagccgcat
    19501 ttagtttcta ttcgtcgcgc gcttcaggtc gtgacttctc tttatcgtta agcaggtgca
    19561 tttaacttga aatggttaat tcttattctg ggcattgcat gtaatgcctc ggctagcgtt
    19621 ctgatcaaaa tggcaatgat gcctccgcgt aaatttcctt ccctgggaga tccattagcg
    19681 tcgttgagta actggccttt ctggctgggc ttgactcttt atggtggtgc gttcttattc
    19741 tatgcgggtg cgctagccgt ttttcctctg aacattgcac atcctgtttt gacaactggt
    19801 gcggttgcag cagtggcagt tttatccgtc ttggttttca atgaaacctt tcaatggacg
    19861 acaattgctg gcgtggtgtt tgtcatcgtc ggtgttgcat taattacgtc aaaagctgct
    19921 taataatgga tttatcgatg aaacatgaaa aaaattggga agtaccgtcg tacgatgtta
    19981 ccttgtggac ggaaaaaaaa catgacgcat gtgtcgtgat cccggtgatt aatgaaggaa
    20041 gccgaattat tagtttactc acgaaaatga gtaatcttaa aattaataat attgctgata
    20101 ttattatcgt cgatggcggc agcactgacg gttctcttga aaaagatcgc ttgttatcgc
    20161 tcggagtttc tggattggta cttaaaatcg gtccaggaaa gctgagtgcg caattacgtt
    20221 gtggttatgc gtttgtactt gaacagggtt ataaaaatat cattactatt gacggtaatg
    20281 ataaggatga tccgtctcca atccctgaat tccttaacgc actggaaaaa ggctatgatt
    20341 ttgttcaggc ttcgcgcttt atcaatggtg gtgttgcggt aaatacacca aaactgcgtg
    20401 atttcgcgat caaatatatt cacgctccag ccctcagcat agcatctggt tttcactgga
    20461 ccgataccac acaaggtttt cgtgcctata gcagcaaatt gctgctagat ggccgtgtgg
    20521 ctcctttccg taatatcttt atgacctacg aactattagc ctatctttct tatattgcac
    20581 caaaattagg ttttcgctgc attgagcttc caacttctcg tatataccca aatggtgatg
    20641 tgccaactaa aattagcggc tttaacggaa atctgagtgt tttgataata ctttttaaaa
    20701 catgttttgg acgatacaat tctaatcggt gtaactaatt aaaactattt ttttttgatg
    20761 cgaattttgg tttttacaat gtatgaataa ttgggtgtcg ttatgaatat ttttggcttt
    20821 gaccacgagc gtgtaaaaat tgaagttgtt cttctgatta tatgtgtggg aggaatgctg
    20881 tgttcaattt atgcatttta tcctgggtgg atgagtccgg attctatctt tcaatataac
    20941 aatgcggtta caggatatta taattcatgg catcctgtca ttatggcttg gtggtggaga
    21001 gttcttggtt atataacaga tggtccgggg ccttttttag tccaaaattt aatgctgttc
    21061 tggattggat tctattttat agctaagacc ttatcaaggt acatcggttg gggtagtata
    21121 gttgtattac tattggctgc aacaccttca gttttaataa ttatggcgca gctatggaag
    21181 gatgcagtat ttacttcact ggcaatattt ggcttaggca tcatctttcg ctctatagat
    21241 acagggagat tttataagag tgtttttttg ctttttgttt ttgtgctctc tcttgctgtg
    21301 ggatgtaaac caaatggtct gtttgttgcc attatattga tagggtggtg gtttattgct
    21361 aatcctaaag tgagtactaa aaggtctaag ttaatacttt cattgacctc tgtattttta
    21421 gttatggcaa taccattttc tatttctaaa tgtcttcctg tttataaaat atcaccgata
    21481 caatatatac agtcatatga tttacttggt ataagtgtaa tagaaaataa aacccttctt
    21541 cctgattata ttactaaaaa agttgggttg agtaaggata atgcccggga gttttacttt
    21601 ccagggggga ataatttgat gttttacaac tcaccagctg gtaacatagc gacgacagat
    21661 cctgaatatt taaaggattt acaatccaga tggatatccg caataaaaga ctatcctatg
    21721 gcatatatta aggttcgatt tgataatttt atagaattac ttcgttgggg cgctattcat
    21781 cccgcttctg ttgcggctga tgttatcgta aataatccat gggggtatgt ttttattccc
    21841 aacaaattaa gtgataatta tattgattca atagataaat accctttttt gtatttccca
    21901 tggatttatt tggttacatc tgctttatta ttcttgttaa caaaggtttt accaagagag
    21961 ttgcgatttt ttaatgttgt tcttgtactt gttgtctttt cgttttcgct tcctcatttt
    22021 tttattgctc cagctgctga ttatcgttat ctgcattttt cagttgtttg tgcagtggtt
    22081 caattaggaa tgtttttggg ctttttcttt atgagattgt ataaaagagt gtattttggg
    22141 gcgattaaat tgttgggtta agggtgttct tctgttatca catattttat ctatatattt
    22201 ataggtttat tagattgggt aattaagtgt tgtctgtatc ttggtgctct tgttgaatat
    22261 gttttttagc ccagctctaa ttgtctgtgc ttttttgcct aaattattat tgatttttac
    22321 tttttcctta atattaactt aataaatgag gtcatatagt aatgaagtaa atctgaattg
    22381 tgccgtcggt cattgacatt tcatcaactc agacgttaca acttttaata catgtcatga
    22441 tgatgcaaac atgatgagca aaagttaacc ctgagaaaat taaatgaaaa tcactatttc
    22501 tggtaccgga tatgttggcc tgtcaaatgg tattctgatt gctcagagcc acgaagtggt
    22561 ggcactggat atcatccaga cgaaaatcga gatgctgaat cagaaacaat ccccgattgt
    22621 ggataaagag atcgaagagt atttggcgac taggtcgcta aacttccgtg caacgactga
    22681 taagtgtgat gcctacttag atgcggacta cgttattatc gcgacaccaa ctgattatga
    22741 tccaaagact aattacttta atacctcaag tgtcgagtct gttattcgcg acgtggttgc
    22801 aattaatcca gatgcagtaa tggtaattaa gtctaccatc ccagtaggtt ttactaattc
    22861 tatcaaagaa aaactgggca ccgaaaatat tttcttttcc ccagaattcc tgcgtgaggg
    22921 taaagcacta tatgacaacc tttacccatc tcgtattgtc attggtgagc gttcagagcg
    22981 cgccgagcgt tttgcagctc tgctacagga aggggcacta aagaaagata ttccgaccct
    23041 atttaccgac tcgaccgaag ccgaagctat taagctgttc gctaatacct atctggcgct
    23101 gcgtatagcc tactttaatg agctggacag ctacgctgaa actctaggcc tgaattcact
    23161 acagataatt gaaggggtat gcttggatcc gcgaatagga aaccactata acaacccgtc
    23221 atttggctac ggcggttact gtctgcctaa ggataccaag cagttgctgt ccaactatga
    23281 atcggtaccg aataacttaa tcggtgcgat cgtcgaagct aatcgcactc gtaaagactt
    23341 tattgccgac tctattctcg cgcgcaagcc taaggtggtg ggtgtttatc gcctgattat
    23401 gaagtcgggt tcggataact tccgcgcgtc ctccattcag gggatcatga agcgtattaa
    23461 ggccaagggt atcacggtaa ttatttatga accggtgatg caggaagatg agttttttaa
    23521 ctcccgcgtg gtacgtgact tcgaagcctt taagctggag gcagacgtga ttatctccaa
    23581 ccgtatggca gaagagctgg tggatgttgg agataaagtc tatacccgtg atctgtttgg
    23641 taatgattaa acgttagttt ttgtgtcgtt ctcggcggtg cttcgcttgg ccgggctatg
    23701 ttagaaaaag ccctcatctt ggactgcacc ccaaaagttg gacacccaac tgattaaggt
    23761 gcagtttttt atggcaaagc caaaatattc tctcgaaacc agattagctg tcgttactca
    23821 ttatcttgcc ggacacgatg gcgcacgtcg taccgctgaa cgttttggtg ttgaagaaac
    23881 atcagtgcga cgcagggtaa gagcctggca actccacggt attgatggta ttacatggaa
    23941 aaatgaccgc cattctcctg agtttcgact gacggttgtc ctgaagtctc tgagtgaaga
    24001 acttaccatg cgcgaagccg ccgcacggtt taatatctcc aatgaatccg ttgtccggca
    24061 ctgggtgaag gtttacaaag acactagtga agcaggactt ctgaacataa aaccaggccg
    24121 gagcaaggac atgacaaaac ctctaaaaac acctccactt acgatgaaga attggaaaag
    24181 ttatctcccg aagaactgcg ggcggaactc cgttaccttc gcgcagagaa tgcctatcta
    24241 aaaaagctga aggccttagt tcaaagcgag aaaaacggca gaaagcccgg ataatcagtg
    24301 agctaaggct gaagcatgcg ctcggtgatc ttctgcgtgc cgcgggtatg tcccgcagta
    24361 cgtggtatca caatatgaat gccctgaagc gagtggacag gcatgccgga ctgaaagaca
    24421 aatcagagag atttatcatt atcacaaagg gcattatggc taccgcagaa tcacgctctc
    24481 actgagaaaa tagggcgtgt tggtaaacca taaaacagtg cagcggctga tggcagagct
    24541 gtcgctccgc tctgtgataa gggtgaagaa atatcgttcc tggaaagggg aagtaggcaa
    24601 agctgcaccc aatatcctga tccggaactt tggtgcaacg aagtccaacg aaaaatgggt
    24661 gacggatgtt acagagttct cggtacaggg taaaaagctg tacctgtcga cagttctcga
    24721 tctgtttaac cgggagatta tctcctacag cctgtcagaa aggctggtga tggagatgat
    24781 taatacgatg ctgcacgatg cattctcagt gtttggaccg gaggatgcac cgttgctgca
    24841 cacggttcag ggctggcaat atcgaatggc aggctatcag gcaacgttaa aggcccatgg
    24901 aatgacacaa agtatgtcgc gcaagggaaa ctgtctggat aacgcagtga tggagaactt
    24961 cttcggcgca ctgaaatcgg agtgttttta cctgaacagg tttagcgatc tgaatgaact
    25021 gaggaaagcg atagaggact atatccatta ttataacaac gagcggatca gcctgaaatt
    25081 aaaaggcctg agtccggtag aataccgaac tcaggccctg aaagccgctt aacatgaact
    25141 gtccaacttt atgggggcag tccatccatg agggcttttt tttgtgcaca gaaaatcccc
    25201 agctaggctg aggggggggg gccggaaagc tttcagcttt gagccagtta ttaaaaccct
    25261 tttgatttgt taaaacacct tgcggtctgg caactgcaag tgtcaaacaa gaaatcaaaa
    25321 gggggggcta atggggaaca aagagagctt agcgcacacc cgattgaacg ataaatctca
    25381 catatttttt gcgccaaaat accgaagaca ggtggtaagg taatcccccc gaaaaaaccg
    25441 gtgcgatgtc cgggttagga aagaggatta ctgatgtctt ttgcgtaaat tctcaatcac
    25501 cgtcgtcagg tctaggttct gatcctgcaa cagcaccagc aggtgataca tcaagtccga
    25561 tgcctcgttg gtcagttcga agcggtcatg tactgtggcg gccagtgctg tttccacgcc
    25621 ttcttcgccc actttctgcg caatgcgttt ggtgccgctg gcatacagtt tggcggtata
    25681 ggaggtttcc ggatcggcag atttacgttc ggcgagcagt tgttccagtt gatacaggaa
    25741 cagccattgg tgagcggtgt cgccgaagca gctgctggtg cctttgtggc aggtcgggcc
    25801 gatgggattc gccagcaaca gtaacgtgtc gttgtcgcag tccggcgtaa tatttactac
    25861 gttgaggaaa ttgcccgacg tttcgccttt ggtccacagt cgctgtttag tgcgcgagaa
    25921 gaaggtgact ttgccgcttt cgatggtttt atctaaggct tccgggttca tatagcccag
    25981 cattaacact tcaccggata ccgcgtgttg cacaatcacc ggcataagtc cgtcggtttt
    26041 ttcccagtcc agttcgcggc gttgttgttc tgttaacata tcctgatctc cacgccctgt
    26101 gttgccaggt acgcttttaa ttcaccaata ttgattattt gtttgtggaa tacggaagct
    26161 gccagcgcac cgtcaacgtc ggcatcgcgg aaagcttcga ggaagtgttc catggtgccc
    26221 gcgccaccgg aggcaattag cgggacgtgg caaacttcac gtactttttt cagttgttcg
    26281 aggtcgtaac cgttacgcac gccgtcctga ttcatcatgt tgaggacgat ttctccggca
    26341 ccgcgttttt gcacttcctg tacccagtcg agcgtttccc actgagtgac gcgggtgcgg
    26401 ctttcat
//
