LOCUS       JX087966               12783 bp    DNA     linear   BCT 25-SEP-2012
DEFINITION  Escherichia coli strain O102 O antigen gene cluster, complete
            sequence.
ACCESSION   JX087966
VERSION     JX087966.1  GI:406066222
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Perepelov,A.V., Wang,Q., Senchenkova,S.N., Gong,Y., Shashkov,A.S.,
            Wang,L. and Knirel,Y.A.
  TITLE     Structure and gene cluster of the O-antigen of Escherichia coli
            O102
  JOURNAL   Carbohydr. Res. 361C, 73-77 (2012)
   PUBMED   22982615
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Perepelov,A.V., Wang,Q., Senchenkova,S.N., Gong,Y., Shashkov,A.S.,
            Wang,L. and Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, No. 23 Hongda Street, Tianjin,
            Tianjin 300457, China
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O102"
                     /db_xref="taxon:562"
     misc_feature    1..12783
                     /note="O antigen gene cluster"
     gene            707..1792
                     /gene="rmlB"
     CDS             707..1792
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AFS33309.1"
                     /db_xref="GI:406066223"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPYPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1792..2694
                     /gene="rmlD"
     CDS             1792..2694
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AFS33310.1"
                     /db_xref="GI:406066224"
                     /translation="MNILLFGKTGQVGWGLQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPEIIVNAAAHTAVDKAESEPEFAQFNLTQQVSKRLRKQQMKLELGLFH
                     YSTDLRLPGNGDMPWLETDATAPLNVYGETKLAGEKALQEYGAKHLIFRTSWVYAGKG
                     NNFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVA
                     SGTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNF
                     ALVLPDWQVGVKRMFNELFTTTAI"
     gene            2752..3630
                     /gene="rmlA"
     CDS             2752..3630
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFS33319.1"
                     /db_xref="GI:406066233"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQLSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLQPSARGELEITDINRISMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3635..4183
                     /gene="rmlC"
     CDS             3635..4183
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AFS33311.1"
                     /db_xref="GI:406066225"
                     /translation="MNVIKTDIPDVLIFEPKVFSDERGFFFESFNQKVFEEAVGRKVK
                     FVQDNHSKSSKGVLRGLHYQLEPYAQAKLVRCMVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKHQLWIPEGFAHGFLVLSNTAEVLYKTNNYYNKENERNIIWNDNTIDIDWPEL
                     NINVILSTKDKCASHINKIDYF"
     gene            4252..5439
                     /gene="wzx"
     CDS             4252..5439
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFS33312.1"
                     /db_xref="GI:406066226"
                     /translation="MPALGYIARELGAERFGLFVLCTAIVGYASIFDAGLTRAVIREI
                     AFNRTNKSELNKIIATSNFAIIILGLVGAALLFMNIDNLVSLLKVSPRYKDDVVSSFK
                     ILTITIPLFLLNQIFVSYLEGMEDFFNINIQRCISSSLVAGLPAFFIILHPTLTCAMI
                     GLVCGRMVSLIISYYCAKEIVLTAGIRFHKQVLFRLLKFGGWITVSNIISPVMSYFDR
                     FILSNLLGAKSVGFYIAPSEFINRLINIPWALARALFPKLSAAKTLSERKKNETIAYI
                     MLSMVCIPAVIIGVGLSYTILNIWLGKEYAENSTYILQVLLIGFLFNSYAQIPFATIQ
                     AAGKAKITALIHCIEIIPYLVGLYFFIIHFGVIGAAYAWTIRVSLDFVILLVASRGIY
                     KSW"
     gene            5433..6461
                     /gene="welL"
     CDS             5433..6461
                     /gene="welL"
                     /note="WelL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFS33313.1"
                     /db_xref="GI:406066227"
                     /translation="MVKKIIHLVKNDYFLTEIIFSKLSSEKIYFYNLTEITKICATKN
                     ILVNLYKSRIKPLFLIVIAYIKRKIHSKNDHAIYFGIEDYKGISSCVYKFSFCANQAI
                     WLWNPRRTLNISEIEFKILLITLKITGVQVWTFDYNDSVKYKLNYHPQVYSLEFSRQV
                     LNKKLCEELTNPVMFDIFFVGVDKGRLELLNTFAKLLDDASLSFSISVLPDKNKHYDE
                     CERLLAKNAMNYDEYLDKLFQTTAILEIVQPGQGGLTIRTLEALFYNKKLITNNESIT
                     EYDFYNADNIFIYKEQDCNIDALKSFLKKNNVIIDQNILRKYDINHLINEILQSPYSS
                     NLNERKSN"
     gene            6442..7572
                     /gene="wzy"
     CDS             6442..7572
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFS33314.1"
                     /db_xref="GI:406066228"
                     /translation="MNENQINKQRSGKYLTAFLCCAFLSINSLAINPAYIYGAGLLSI
                     IIMLIFVKAKISKFSMVTIIFAFAILLCQVIGIYYKTQTTGYPEAPNYITPFLFFYSM
                     VISVGVNEVFMVLNYDTRIKCYKNFLMFFVMFMFLELITRVIIGNLSEGILYAFKKSL
                     FYFDSNFTALIDISILGFLLFLKNNKIYNFKYTRYITYILLFLTMSRAAMVTLLAMLY
                     VLNNKAKIGKRSALVFLFVICVFIYMSISHLFENSNYSNIDGSFNSKFYIISQAFEFY
                     TTQSLMTHMFGVGLGNTEQLLGIFAHNIYVTLVLEFGFIGSFLFITFIFYSLKKTNFN
                     SLYIWLPVCISGISLFGAYSPFLFILNAVIYNETRYLGNQKL"
     gene            7569..8501
                     /gene="welM"
     CDS             7569..8501
                     /gene="welM"
                     /note="WelM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFS33315.1"
                     /db_xref="GI:406066229"
                     /translation="MKNEPLISILIPTYNVGAWIDEAIKSIIEQTYKKIEIIIIDDYS
                     TDNTREKIQNLARTDSRIKIIYNKENQKIVKSLNQGLLLAKGQYIARFDGDDIAAPDR
                     IEKQLHYLEANDLDIVGCQMHSINEIGEHLTKSISPVGEDIVKKVSKYVSPIAHIWLA
                     KREVYDELQGYREIPYAEDYDFILRALDRGYKCDNHPEYLMYIRHRQGNSASVASLLQ
                     RKAHNYVLKLHKLRIRRGRDNFDVLSMHKKLSSSILIEKLHSTSSRCLALAVQQKSMY
                     RRFFFIFLTVLLSIYNMQYLYRRLIVRNIMRNKK"
     gene            8498..9550
                     /gene="welN"
     CDS             8498..9550
                     /gene="welN"
                     /note="WelN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFS33316.1"
                     /db_xref="GI:406066230"
                     /translation="MKNKISFIISDITNKGGTERVTALLSNILSSNIENAEVEIFSLQ
                     KKHANPFYFLSNKISVKYGNGWRYYSLLSYLIFCKKNKHKVIVESMGRLSLECGILAK
                     AIRLHDIYFHEHVGLNSFKKAIQLIKIFSYYMAKKVIVLTSHDYNLLTTKYGIKNVIQ
                     IPNINPFDTSNNIIKPYDQRENIVIAVGRFTEQKNFNELIEIWHEANIPNWTLYIIGD
                     GPNKRILEKAIAGMSSIKMFKTTDEICNYYNAAKIYAMTSLYEGLPMVLIESQSFGIP
                     SVAYDCPTGPAEIITDNVNGFLIPFKNRELFIQKLRKLTSDDNLSNIFSTNAIKNSRK
                     YNADAIIHKWISLLGI"
     gene            9554..10291
                     /gene="welO"
     CDS             9554..10291
                     /gene="welO"
                     /note="WelO"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFS33317.1"
                     /db_xref="GI:406066231"
                     /translation="MVRETVSIIMPAYNASATIEQAVNSVIDQDFKDWHLYIIDDASH
                     DNTFELADKFQDERITVIKNRNNKGVAESRNIALNICKGNYVAFIDSDDMWIKNKLSS
                     QMEKLEDGWDLVCSNFITFSNEKEYINARISPEIITYDMMLKSNFIGNLTGIYNREKL
                     GIFNQVNEGHEDYIMWLDIISVTNRAYCIQECLAKYRVNEHSLSSNKIKAIAWQWNIY
                     RKNRRLSFLHSIKYIIYYFFHALLKRV"
     gene            10330..11349
                     /gene="gne"
     CDS             10330..11349
                     /gene="gne"
                     /note="Gne"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-4-epimerase"
                     /protein_id="AFS33318.1"
                     /db_xref="GI:406066232"
                     /translation="MKLLVTGGLGYIGSHTVVSLLDAGYEVIIIDNLSNSHLSVLKRI
                     NNICLRKASFIEGDIRNRTVLNQIFNSYNIDAVIHFAGLKSVGQSISSPISYYDNNVV
                     GTLALLEEMLRHDVMRFVFSSSATVYGSPKSIPIKESNEVGHTTNPYGTSKLIIEKIL
                     SDICYSNSSFRCTILRYFNPVGAHTSGLLGEDPRGKPNNLMPYICQTAIGKLNEVHIF
                     GNDYNTHDGTGVRDYIHVMDLAEGHVAAVKASNEGENCKIYNLGTGKGYSVLDLIKTF
                     EQITNVAVPHIFVGRRPGDIAECWSDPSKAYEELNWRAKKDLADMIRDAWKWQVQNPN
                     GYSDD"
BASE COUNT     4052 a   2058 c   2630 g   4043 t
ORIGIN
        1 taaagtcagt cgcaccgtcg aatttatcga aaaaccggat cagccgcaga cgctggactc
       61 agatatcatg gccgttggtc gctatgtgct ttctgccgat atttggccgg aacttgaacg
      121 cactcagcct ggtgcatggg ggcgtattca gctgactgat gccattgccg aactggcgaa
      181 aaaacagtcc gttgatgcca tgctgatgac cggcgacagc tacgactgcg gtaaaaaaat
      241 gggctatatg caggcgtttg tgaagtatgg actacgcaac ctgaaagaag gggtgaagtt
      301 ccggaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga tgtaacggtt
      361 gataagaaaa ttataacggc agtgaagatt cgtggtgaaa gtaatttgtt gcgaatattc
      421 ctgccgttgt tttatataaa caatcagaat aacaacgagt tagcaatagg attttagtca
      481 aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta acgtttgaat
      541 ttttcgggtt tagcgcgggt gggtaacgcc cgtcacatcg taggcatgca tgcagtgctc
      601 tggtagctgt aaagccaggg gcggtagcgt gcattaatac ctctattaat caaactgaga
      661 gccgcttatt tcacagcatg ctctgaagta atatggaata aattaagtga aaatacttgt
      721 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      781 ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      841 tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg ctgctgcaat
      901 ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg ctgaaagcca
      961 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtactta
     1021 tgtcctttta gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaaatag
     1081 tttccgtttt catcatattt ctactgacga agtctatggt gatttacctt atcctgacga
     1141 agtaaataat aaagaagaat tacccttatt tactgagaca acagcttacg caccaagcag
     1201 cccttattcc gcatcaaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
     1261 tggtttaccg accattgtga ctaactgctc taacaattat ggtccttatc atttcccgga
     1321 aaaattgatt ccattggtta ttctgaatgc tctggaaggt aaagcattac ctatttatgg
     1381 taaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
     1441 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
     1501 aaatatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc cgaaagagaa
     1561 atcttaccgc gagcaaatta cttatgttac cgatcgtccg ggacacgatc gccgttatgc
     1621 gattgatgct gagaagattg gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag
     1681 cgggattcgt aaaacggtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1741 aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
     1801 ctcctttttg gcaaaacagg gcaggtaggt tggggactac agcgtgctct ggcacctttg
     1861 ggtaatttga ttgctcttga tgttcactct actgattatt gtggtgattt tagtaatcct
     1921 gaaggtgtag ctgaaaccgt aagaagcatt cggcctgaga ttattgtcaa cgcagccgct
     1981 cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaatttaa tttaacgcaa
     2041 caagtgtcga agcgattgcg aaagcagcaa atgaagttgg agcttgggtt attccattac
     2101 tcgactgatt tacgtcttcc tggaaatggc gatatgccat ggctggagac ggatgcaacc
     2161 gcaccactaa atgtttacgg tgaaacaaag ttagctggag aaaaagcgtt acaggaatat
     2221 ggcgcgaagc atcttatttt ccggaccagc tgggtctatg caggaaaagg aaataacttc
     2281 gccaaaacga tgttacgtct ggcaaaagag cgtgaagaat tagcggttat taacgatcag
     2341 tttggtgcgc caacaggtgc tgaactgctg gctgattgta cagcacatgc cattcgtgtc
     2401 gcactgaata aaccggatgt cgcaggcttg taccatttgg tagccagtgg taccacaacc
     2461 tggtacgatt atgctgcgct ggtttttgaa gaggcgcgca aagcaggcat tccccttgca
     2521 ctcaacaagc ttaacgcagt accaacaact gcctatccta caccagctcg tcgtccacat
     2581 aactctcgcc ttaatacaga aaaatttcag cagaattttg cgcttgtctt gcctgactgg
     2641 caggttggtg tgaaacgcat gttcaacgaa ttatttacga ctacagcaat ttaatagttt
     2701 ttgcatcttg ttcgtgatgg tggagcaaga tgaattaaaa ggaatgatga aatgaaaacg
     2761 cgtaaaggta ttattttagc gggcggttct ggtactcgtc tttatcctgt gactatggct
     2821 gtcagtaaac agctattacc tatttatgat aaaccgatga tctattaccc gctctctaca
     2881 ctgatgttgg cgggtattcg cgatattttg attatcagta cgccacagga tactcctcgt
     2941 tttcaacaac tgctgggtga cgggagccag tggggcctga atcttcagta caaagtgcaa
     3001 ctgagtccgg atggtcttgc gcaggcattt atcatcggtg aagagtttat cggtggtgat
     3061 gattgtgctt tggtacttgg tgataatatc ttctacggtc acgacctgcc gaagttaatg
     3121 gatgccgctg ttaacaaaga gagtggtgca acggtatttg cctatcacgt taatgatcct
     3181 gaacgctatg gtgtcgttga gtttgataaa aacggtacgg caattagcct tgaagaaaaa
     3241 ccgctacaac caaaaagtaa ttatgcggta accgggcttt atttctatga taacgacgtt
     3301 gtggaaatgg cgaaaaacct tcagccttct gcccgtggtg aactggaaat taccgatatt
     3361 aaccgtattt ctatggaaca ggggcgttta tccgttgcca tgatggggcg tggttatgca
     3421 tggctggaca cggggacaca tcaaagtctt attgaagcaa gcaacttcat tgctacaatt
     3481 gaagagcgtc aggggttgaa agtttcctgc ccggaagaaa ttgcttaccg taaagggttt
     3541 attgatgctg agcaggtgaa agtattagct gaaccgctga aaaaaaatgc ttatggtcag
     3601 tatctgctca aaatgattaa aggttattaa taaaatgaac gtaattaaaa cagatattcc
     3661 agatgtactg atttttgaac caaaagtttt tagcgacgag cgcggtttct tttttgagag
     3721 ctttaaccag aaggttttcg aagaagcggt aggccgtaaa gttaagtttg ttcaggataa
     3781 ccattcgaag tcaagtaaag gtgttttacg tggtcttcat taccagttag aaccctatgc
     3841 tcaagcaaaa ctggtgcgct gtatggtcgg tgaagttttt gatgttgcgg ttgatattcg
     3901 taaatcttcg cctacctttg gaaaatgggt tggggtgaac ttatctgctg agaataagca
     3961 tcaattatgg attccggaag ggtttgctca tggattttta gtattatcta acactgctga
     4021 ggttttatat aagacgaata attattacaa taaagaaaat gaaagaaata ttatttggaa
     4081 tgataataca attgacattg attggcccga attgaatata aatgttatcc tttccactaa
     4141 agataaatgt gcatctcaca ttaacaaaat tgattacttt taggaaaata tgagtttatt
     4201 aaaaatagta tatggaactt tggtggatac attattccaa ccattgtcgc tttgcctgca
     4261 ttaggatata tagctcgtga attgggagca gaaaggtttg gcttgtttgt tctttgtaca
     4321 gctattgttg gttatgccag tatttttgac gcaggattaa caagagctgt tattagagaa
     4381 attgcgttta atagaactaa taaatccgaa ttaaataaaa ttattgcaac atctaatttt
     4441 gctataatta tactgggttt agttggtgca gctcttttgt ttatgaacat tgataaccta
     4501 gtgtctttgt taaaggttag ccctagatat aaggatgatg ttgtatcttc gtttaagata
     4561 ctgactataa ctataccttt atttttgcta aatcagatat ttgtatcata tttggagggc
     4621 atggaagatt tttttaatat aaatattcaa cgatgtatta gtagttcatt agtagcgggg
     4681 cttccagctt ttttcatcat tctacatcca accctgacat gtgcaatgat tggattagtt
     4741 tgtggtcgaa tggtttcgtt aattatttca tattattgtg caaaagaaat agtattaaca
     4801 gcgggtatta gatttcataa gcaagttttg tttcgactgt taaagtttgg tggctggatt
     4861 actgtaagta atattattag tccggtcatg agttattttg atcggtttat attatctaat
     4921 cttctggggg caaaaagcgt tgggttttat atagcgcctt cagaatttat taatagattg
     4981 attaatattc cctgggcttt ggcaagagca ttatttccaa aattgagtgc tgcaaagaca
     5041 ctttcggaaa gaaaaaaaaa tgaaacaata gcctatatta tgctcagcat ggtttgcata
     5101 cctgctgtga ttattggagt gggattatcg tatacaatcc taaatatatg gttaggtaaa
     5161 gaatatgctg aaaattcaac ttatattttg caagttcttc tgataggatt tttatttaat
     5221 tcatatgctc agattccatt tgctacaatc caggctgcag gtaaagcgaa aatcacggca
     5281 ttaatacatt gcattgaaat aattccttac cttgttggat tgtatttctt tattatacat
     5341 tttggtgtaa taggggctgc atatgcgtgg acaattagag tcagtttaga tttcgttatt
     5401 ttacttgtag catctcgggg catatataaa tcatggtaaa aaaaataata catttggtca
     5461 aaaatgatta ttttttaacg gagattattt tttcaaagtt atcttcggaa aaaatatatt
     5521 tttataattt aactgaaatc acaaagattt gcgcaactaa aaatattctt gtcaatttgt
     5581 ataaatcaag gataaaacca ttatttttga ttgtcattgc gtatataaaa aggaaaattc
     5641 actcaaaaaa tgatcatgca atttattttg gtatagagga ttataaagga atatcttctt
     5701 gcgtatataa gttttcgttt tgtgccaatc aagccatttg gttatggaat cctagacgaa
     5761 cattgaacat ctcagaaata gagtttaaga ttctattaat cacattaaaa attactgggg
     5821 ttcaagtttg gacgtttgat tataatgact cggttaaata taaactaaat tatcatcccc
     5881 aagtttactc attggaattt agtcgtcaag tattaaataa aaaactgtgt gaggaattaa
     5941 ccaatcctgt aatgttcgat attttctttg ttggggtaga taaaggtcgg cttgaacttt
     6001 taaatacctt tgcaaagtta ttagacgatg cttccttgag cttttcaatt agtgttttgc
     6061 ctgataaaaa taagcattat gatgaatgtg agagactatt agcaaaaaat gcaatgaatt
     6121 atgacgagta tcttgataaa ttatttcaaa caacagctat tcttgaaatt gttcaaccgg
     6181 gacaaggagg tctgacaatt agaacattgg aagccctatt ctataataaa aagttaataa
     6241 caaataacga aagtattacc gaatatgatt tttataatgc agataatata ttcatatata
     6301 aggagcagga ttgtaatatc gatgcgttaa aatcattttt aaaaaaaaat aatgtaatta
     6361 tagatcaaaa tatacttaga aagtatgata ttaatcatct tattaatgaa attctacagt
     6421 caccatattc gagtaatttg aatgaacgaa aatcaaatta acaaacaaag atccggtaag
     6481 tatcttacgg catttttatg ttgtgcattt ttgagcataa attctcttgc tattaatcct
     6541 gcttatatat atggtgcagg gttgttaagt attataataa tgctaatatt cgttaaggca
     6601 aaaatttcaa agtttagcat ggtaactatt atttttgctt tcgcaattct tctgtgtcag
     6661 gttataggta tctattataa aacccaaaca acagggtatc ctgaagctcc aaattacatc
     6721 acacccttcc tgttttttta ctcgatggtt attagcgttg gagtaaatga agtatttatg
     6781 gtgctaaact atgatacacg aattaaatgt tataaaaatt ttttgatgtt ttttgtaatg
     6841 ttcatgtttc tggagttgat tactagagtc attataggta atctatcaga aggtatttta
     6901 tatgcattca aaaaaagttt attctacttt gattcaaatt ttacagcatt aatagatatt
     6961 tccatcttag gttttttatt gtttcttaaa aataataaaa tctataactt caaatataca
     7021 aggtacataa catatattct gctattttta acaatgtcaa gagctgcaat ggtaacgctg
     7081 cttgctatgt tatatgttct gaataataag gcaaagatag gaaagcgttc tgcacttgtt
     7141 tttttatttg tcatatgtgt atttatttac atgtcaataa gccacttatt tgaaaatagc
     7201 aattattcaa atattgatgg gagttttaat tctaaattct acattattag ccaagcattt
     7261 gaattttaca ctacgcaatc acttatgaca catatgtttg gtgttgggct tggaaacaca
     7321 gaacaattac tagggatttt tgcacacaac atatatgtaa ctttagtttt agaatttggt
     7381 tttattgggt cttttctttt tataactttt attttttata gccttaaaaa gacaaatttt
     7441 aattcattgt atatttggtt gccagtatgt atcagtggta tttcgctatt tggtgcatat
     7501 tcaccatttt tatttatatt gaatgctgtt atttataacg aaaccagata tttaggtaat
     7561 caaaagttat gaaaaatgaa ccattaattt caatattaat tccgacgtat aatgtcggag
     7621 catggattga tgaagccata aaaagtatta ttgaacaaac ttataaaaaa atagaaataa
     7681 taataattga tgattattca actgataata cgcgggagaa aatacaaaat ctagcgcgta
     7741 cagatagtag gattaaaata atatataata aggaaaatca gaaaattgta aaatcgttaa
     7801 atcaggggtt attgttagct aaagggcaat atattgcgcg atttgacggg gatgatatcg
     7861 cagcacctga tagaattgaa aaacaattgc attatctcga ggcgaatgat ttagatattg
     7921 ttggatgtca aatgcactca attaatgaga taggtgagca tttgacaaaa agtatctcac
     7981 ctgttggaga agatatagtt aaaaaggtgt ctaaatatgt ctctccaatt gctcacattt
     8041 ggttagctaa gagggaggta tatgatgagt tacaaggtta tagggaaata ccttatgctg
     8101 aagattatga ttttatatta agagctttgg atcggggtta taaatgtgat aatcaccctg
     8161 agtatctaat gtacattaga catcgacagg gcaatagtgc aagtgttgct agtttacttc
     8221 aaagaaaagc gcataattac gtgttaaaac tccataaact tagaattaga agagggagag
     8281 ataattttga tgttttgagt atgcataaaa agctaagttc ctctattctt atcgagaaac
     8341 tccattctac ctctagtcgt tgtttagctt tagcagtgca acaaaaatca atgtatagaa
     8401 gatttttctt tatatttctt acagtattgt tatcaattta taatatgcaa tatctttata
     8461 gaagactaat agttcgtaat attatgagaa acaaaaaatg aaaaataaaa ttagtttcat
     8521 tatttctgat attactaata agggcggtac agaaagagtt acagcattat tatcaaatat
     8581 attatcaagt aatatagaaa atgctgaggt cgaaattttc tcattacaaa aaaaacatgc
     8641 caatcctttc tatttcttat caaataaaat ctctgttaaa tatggaaatg gatggcgcta
     8701 ttattctcta ttgtcctatt tgattttttg taagaagaat aaacataaag ttattgttga
     8761 gtcgatggga cgcttatcgc tggagtgtgg aatattagcg aaagctatca ggctgcatga
     8821 tatatatttc catgagcatg taggcttgaa tagttttaaa aaagcaatac agctaataaa
     8881 aatattttca tattacatgg caaaaaaagt aattgtttta acttcgcatg attataattt
     8941 attaactaca aaatatggta ttaaaaacgt tattcaaata ccaaatatta acccatttga
     9001 tacatccaat aacataataa aaccatatga ccaacgggaa aatattgtga tcgctgttgg
     9061 acgatttaca gagcaaaaga atttcaatga gttgattgaa atttggcatg aggcaaatat
     9121 acctaattgg acgttatata tcattggtga tggccctaat aaaaggattc ttgaaaaagc
     9181 catagctgga atgtcatcta tcaaaatgtt taaaacaaca gatgaaattt gcaattacta
     9241 taatgctgcc aaaatatatg cgatgacttc gttatatgaa ggattaccta tggtgttgat
     9301 tgagtcccag agttttggaa ttccgagtgt tgcatatgat tgtccaactg gcccggcaga
     9361 aattattact gataatgtta atggttttct tatccctttt aaaaacagag agctttttat
     9421 acagaagtta agaaaattga cttctgatga taatttatca aacattttta gtactaatgc
     9481 tattaaaaat tcaaggaaat ataatgctga tgcaattatt cataaatgga tatcactact
     9541 tggaatataa aatatggtaa gagaaactgt ttcgataata atgcctgctt ataatgcgtc
     9601 tgcaacaata gaacaagctg taaactctgt aattgatcaa gactttaagg attggcatct
     9661 atatattatc gatgatgcat cgcatgataa tacattcgaa ttagctgaca aatttcaaga
     9721 tgaaagaata acagtaatta aaaacagaaa taacaaaggc gtcgcggaat caagaaatat
     9781 tgcactaaat atatgtaaag gcaattatgt tgcctttata gatagtgatg atatgtggat
     9841 taaaaacaaa ctttcttcac agatggagaa attagaagac ggttgggatt tggtatgcag
     9901 taattttata acattttcaa atgaaaaaga atatattaat gctcgaattt caccagaaat
     9961 aataacttat gatatgatgc ttaaaagtaa ttttattggt aacctgacag gtatatataa
    10021 tagggaaaaa ctgggtatat ttaaccaagt aaatgaagga catgaagatt atataatgtg
    10081 gttggatata atatcagtga ccaatagagc ttattgtata caagagtgtt tagctaaata
    10141 tcgtgtaaat gaacattctt tatcctcaaa taaaattaag gcaatagcct ggcagtggaa
    10201 tatttataga aaaaaccgtc gtctttcatt cttacattca attaaatata tcatttatta
    10261 tttttttcat gccttgttaa aacgagtcta acgtgagaac tattaaagtc taagtgaggt
    10321 caagataaaa tgaaattgct tgtaactggt ggattagggt atataggttc tcatactgtc
    10381 gtgtcgttat tagatgctgg ctacgaagtt atcataattg ataatttatc taactcgcat
    10441 ttatcagtat taaaaaggat taacaatatt tgcttaagaa aggcatcatt tatagaaggt
    10501 gacattagaa atagaacagt acttaatcaa atctttaata gttataatat tgatgctgtt
    10561 atacattttg ccggtttaaa atcagtaggt caatcgatta gttcacctat aagttattat
    10621 gataataatg tcgtcggaac tctagcattg cttgaagaga tgttgcgaca tgatgtaatg
    10681 agatttgttt ttagttcatc tgctacagta tatggatcac caaagagtat tcctataaaa
    10741 gagtcaaatg aagttggaca tacaactaat ccatatggta catctaaact aataatagaa
    10801 aaaattctat cggatatatg ttattcgaat agttcatttc ggtgtactat tttgcgttat
    10861 tttaatcctg ttggggctca cacatcagga ttattagggg aagatccaag aggaaaacct
    10921 aataatctta tgccatatat ttgccaaacc gcaataggta aactcaatga ggttcatatt
    10981 ttcggtaatg attataatac ccatgatggt actggtgtgc gtgattatat tcatgttatg
    11041 gatcttgccg aagggcatgt tgcagcggta aaggcatcga atgaagggga gaattgtaag
    11101 atctataatt taggtactgg aaaaggttat tcggttcttg acttaataaa aacttttgaa
    11161 caaataacaa atgtcgctgt accacatata tttgttggta ggcgtcccgg tgacatcgct
    11221 gaatgttggt cagatccttc aaaagcttat gaagaattaa attggagagc taaaaaagat
    11281 ttagctgata tgattcgaga tgcttggaaa tggcaagtgc aaaaccccaa tggatattca
    11341 gatgattaat tattacctat ctagttattc tttttgataa aatagaatag tcttttataa
    11401 tataacattt actataagaa attttccctg acaggagtaa acaatgtcaa agcaacagat
    11461 cggcgtagtc ggtatggcag tgatggggcg caaccttgcg ctcaacatcg aaagccgtgg
    11521 ttataccgtc tctattttca accgttcccg tgaaaagacg gaagaagtga ttgccgaaaa
    11581 tccaggcaag aaactggttc cttactatac ggtgaaagag tttgttgaat ctctggaaac
    11641 gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc
    11701 cctcaagccg tacctcgata aaggtgacat catcattgat ggtggtaaca ccttcttcca
    11761 ggacaccatt cgccgtaacc gtgagctttc tgccgaaggc tttaacttca tcggtaccgg
    11821 tgtttccggt ggtgaagagg gcgcgctgaa agggccgtcc atcatgcctg gtggccagaa
    11881 agaagcctat gaactggttg cgccgatcct gaccaaaatc gccgccgttg ctgaagatgg
    11941 cgaaccgtgc gttacctata ttggtgccga tggcgcgggt cactatgtga agatggttca
    12001 caacggtatt gaatacggtg atatgcagct gattgctgaa gcctattctc tcctgaaagg
    12061 cggcctgaat ctttctaacg aagaactggc acagaccttt accgagtgga ataacggtga
    12121 actgagcagc tacctgatcg acatcaccaa agacatcttc actaaaaaag atgaagacgg
    12181 taactacctg gttgatgtga tcctggatga agcggctaac aaaggtaccg gtaaatggac
    12241 cagccaaagc gcgctggatc tcggagaacc gctgtcgctg attaccgagt ctgtgtttgc
    12301 acgttatatc tcttctctga aagagcagcg tgttgccgca tctaaagttc tctctggccc
    12361 gcaagctcag ccagcaggcg acaaggctga gttcatcgaa aaagttcgtc gtgctctgta
    12421 tctgggcaaa atcgtttctt acgctcaggg cttctctcag ctgcgtgcgg cgtctgaaga
    12481 gtacaactgg gatctgaact acggcgaaat cgcgaagatt ttccgtgctg gctgcatcat
    12541 ccgtgcgcag ttcctgcaga aaatcaccga tgcatatgcc gaaaatccgc agatcgctaa
    12601 cctgctgctg gctccgtact tcaagcaaat tgccgatgac taccagcagg cgctgcgtga
    12661 tgtcgttgct tatgcagtac agaacggtat cccggttccg accttcgccg ctgcggttgc
    12721 ctattacgat agctatcgtg ccgctgttct gcctgctaac ttaattcagg cccagcgtga
    12781 cta
//
