LOCUS       AY532664               12033 bp    DNA     linear   BCT 01-NOV-2006
DEFINITION  Escherichia coli serotype O103 O-antigen gene cluster, complete
            sequence.
ACCESSION   AY532664
VERSION     AY532664.1  GI:45644916
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Fratamico,P.M., DebRoy,C., Strobaugh,T.P. Jr. and Chen,C.Y.
  TITLE     DNA sequence of the Escherichia coli O103 O antigen gene cluster
            and detection of enterohemorrhagic E. coli O103 by PCR
            amplification of the wzx and wzy genes
  JOURNAL   Can. J. Microbiol. 51 (6), 515-522 (2005)
   PUBMED   16121232
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   Fratamico,P.M., Briggs,C.E., Debroy,C., Strobaugh,T.P. Jr. and
            Chen,C.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2004) Eastern Regional Research Center,
            Agriculture Research Service, U. S. Department of Agriculture, 600
            E. Mermaid Ln., Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O103"
                     /db_xref="taxon:562"
     gene            107..1183
                     /gene="rmlB"
     CDS             107..1183
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-glucose 4,6-dehydratase"
                     /protein_id="AAS73163.1"
                     /db_xref="GI:45644917"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDSVAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KGLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            1180..2052
                     /gene="rmlA"
     CDS             1180..2052
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative D-glucose-1-phosphate
                     thymidylyltransferase"
                     /protein_id="AAS73164.1"
                     /db_xref="GI:45644918"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFMRLLGDGSQFGINLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLMLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKCIKPSKRGELEITAVNEVYLRNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNHDELQAKGEFLKKN
                     HYGQYLLRLIKSHE"
     gene            2045..2452
                     /gene="wbtA"
     CDS             2045..2452
                     /gene="wbtA"
                     /note="WbtA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-6-deoxy-3,4-keto-hexulose
                     isomerase"
                     /protein_id="AAS73165.1"
                     /db_xref="GI:45644919"
                     /translation="MNNNVKLIHFDKKGDNRGFLVVVEQNKDIPIEIKRVYYMFDTGS
                     DVRRGYHAHKKLTQIAIPVKGSCKFLLDDGKSVEHVTLDSPSVGLIIEPMIWHEMYDY
                     SSDCVLMVLADDFYDESDYIRNYADFIREMTNA"
     gene            2445..2978
                     /gene="wbtB"
     CDS             2445..2978
                     /gene="wbtB"
                     /note="WbtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative butyryltransferase"
                     /protein_id="AAS73166.1"
                     /db_xref="GI:45644920"
                     /translation="MRNLSLFTKNTKLIPVEINDAEFIYSLRVDSRLNKHISTINGTV
                     IEQAEWIEDYKKREENNEEFYFIIKRTDNDEKIGTIRLYNITKDDGFCWGSWILNDDK
                     MKSSAIESAYLIYKFGFLHKNYKYSYFQVDKKNKEVIAFHKKTGATIIKEDDVNFHFE
                     FYVENFIRLREKYSKYF"
     gene            2975..4096
                     /gene="wbtC"
     CDS             2975..4096
                     /gene="wbtC"
                     /note="WbtC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="AAS73167.1"
                     /db_xref="GI:45644921"
                     /translation="MRGKKMIDFLNLKRINAQYEMELKDACSRVIDSGWYIMGTELKT
                     FEEKFANWCGVKHVIGVANGLDALILVLRAWKEMGKLNDGDEIIVPANTYIASVLAIT
                     ENNLVPVLTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYGQISPMAEIMEIAAEFGL
                     LVLEDCAQAHGSSIDGRKAGSWGHAAGFSFYPGKNLGALGDAGAITTSDDQLADILTA
                     IRNYGSHEKYKNKYQGLNSRLDEMQAAFLNVKLKYLDRENKLRRAIAKKYIEEIKNPL
                     ISLPVIAGNEMSHVWHLFVIRTSERDSLQKHLKTEGITTLVHYPIAPHNQEAYKEYSF
                     LSLPLTERIHREVLSLPMDPTMSIDDINMVIEAINRWKS"
     gene            4084..5349
                     /gene="wzx"
     CDS             4084..5349
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAS73168.1"
                     /db_xref="GI:45644922"
                     /translation="MEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLG
                     QLQNFVAFINGFISSQVSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGV
                     LFSSKISEYLFYQSDLYWLIIIALCALPLNIVNNIFLGVLNGLNEYNRFFLANVLAIS
                     SSLLSMVGLVYFFGLKGALVSASLNNAVAGVWLITIIIKRPWFKFKYWVGHTPRHNIT
                     QMKNYFYMGVIGALTGPISMIVVRTILTNNFSLEDAGYWQAVNRISEAYLAVLTTALT
                     VYYFPKTAAARRYSEYITLLKTGACIVVPLALSMALTIYGLKDFIISILFTADFIRAR
                     ELFLFQNIGDFLRICSWLFATILLAKGYFKINAILEVMFSIIFPVLVKFLIINFGLNA
                     VSIAYSITYALYFIVVCIIYCWHLTKKAL"
     gene            5353..6339
                     /gene="wbtD"
     CDS             5353..6339
                     /gene="wbtD"
                     /note="WbtD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS73169.1"
                     /db_xref="GI:45644923"
                     /translation="MIISSDNLSVIIPVYNEEKNVINILQSLECQSLTGFNVIVIDDG
                     SKDMTASLVKEYKPSSYSLSLIQQSNMGAARARENAINFTHSEYIAFIDSDDSLSSDA
                     LEKALAPMLDNKDIDISLFELVHIKNLNHDTNNIFVPYSTTKLICGEEAFANCISYWG
                     LHGFGIYKRKLIQKSYGIYYKYNKNKENYINNDEVISRISFGLSKNIYLSSGRYFFVQ
                     NMDSTTRRINESYYKVINNAVYLREYIDEEIKYNDFDCLGEANKLLVSTIWGVFVRYQ
                     KWKKKFSDETNGKWREAIKKGMQYIKKINNNKNINLHIKSKMQLYIISKLVS"
     gene            6342..7232
                     /gene="wbtE"
     CDS             6342..7232
                     /gene="wbtE"
                     /note="WbtE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS73170.1"
                     /db_xref="GI:45644924"
                     /translation="MKNLLITIIVASYNSKDYILETLESCINQQYQNIEIIIVDDCSS
                     DNSVELIKQWCKEKKSTSPKIRCILVESERNKGIPANLNNALPFINGDWIKCIGSDDI
                     LLPEAISEFVNRLEKCSNLDNIGAVFTYFQTFGYSVNVSTRYPSSWTRTICQMPPSWL
                     KKAAAMIHFNNVAPCAFINKNHFSGFDTSYKLLEDLPLWLKMIKNNKNTLFFDYTTVL
                     YRLHDSQITSLNNIKVNDVLINDLCRLNYYRKNNGYYLAYLHHKFNLYCAVKRMRFYR
                     YLKIINPLNLLIVLYERVKK"
     gene            7232..8380
                     /gene="wzy"
     CDS             7232..8380
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAS73171.1"
                     /db_xref="GI:45644925"
                     /translation="MYILSMAIPLFFISLCWHRFSHQTQVAFRLLFIVFFSLSFFSYY
                     TNGVDWSVYYLRFIEDEGLFTSFEFGFVIILKLLLIVSDDNFGLAILLYYFLCFVLLS
                     LILKKYKVNEPLFLGCLLLLFGYNLFLEQLRQLLACIIVFYAMLLYNQNKNLIELCIL
                     VITASTFHVSAVIILPTIFLISFRNVTTFIIITVSSITSIVVFMIALYSAISSLAAMS
                     FVFAKIDYYLHQYPVVLNFGWLNILDALFILFYMRYRNAIDRQININILTRIIFVGSV
                     IHLFSGSITFLARVCFYFYFVGVYIYCYSLNDKTPRVFAIKNFNTLVLAAFVNVVLLL
                     NFASYFRNEQAPVGFYNMNFQFFNTFNDNYLRTNAYDKYFKGMQGSVD"
     gene            8383..9474
                     /gene="wbtF"
     CDS             8383..9474
                     /gene="wbtF"
                     /note="WbtF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS73172.1"
                     /db_xref="GI:45644926"
                     /translation="MSKRIIFVITGLGLGGAEKQICLLADKLYLHNNEVNIVVLNGNV
                     NVTPLNENIRIHNLHMKKNPLGLLNAVLRLVKIISYFKPDIVHGHMFHANIVARLAKV
                     FTYNKYKLICTAHSKNEGGHIRMLVYRLTDFLCDATTNVSNEALDVFIKKKAFSKSKS
                     IPVYNGIDTDLFKYDPLQRDKIRHSLNIINDDSLILSVGRLTDAKDYPNLLKALLELP
                     ERYKLVIIGEGEARNEVERTILNYGLEKRVKLLGSIANVATYYSACDIYVSSSKWEGF
                     GLVVAEAMSCQRLVVATNAGGVAEVVGDMNYIVPVSDPQLLANKINEVMEFDHETKME
                     VMYRNREFVLKMFSIDMIVNQWMKLYSSI"
     gene            9660..10781
                     /gene="wbtG"
     CDS             9660..10781
                     /gene="wbtG"
                     /note="WbtG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS73173.1"
                     /db_xref="GI:45644927"
                     /translation="MKVLLIGNQSSTIILFRKAVIENLISKNIKVYTLTMDNNEGNFN
                     KIKKIGAIPLSYSFSRSGINPISDFFNTFFLARKIKEINPDSVLCFFPKPVIYGTLAA
                     MIAGVKKRYALLEGLGFCFTQNKDKDSFKKIVLRKIQGFLYKMTLPFATKVLFLNNDD
                     YHDLVVKNNIKIKDYKVIGGIGVNLNEFSYHPPICGEIHFGMVARLLREKGVCEFVEA
                     AKIIKKKYPMVRFSIAGDFDDNPGGIDAAQIVNWKAEGYVEFLGPCSYIKEYLSDISV
                     FVLPSYREGVPKSTQEAMAIGRAIITTDVPGCRETVIDGYNGYLVPPWDTQKLALVME
                     TFIHERELIVTMGLNSRRFALSKFNESESANSLVNIITA"
     gene            10860..11888
                     /gene="galE"
     CDS             10860..11888
                     /gene="gne"
                     /note="Gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-galactose-4-epimerase"
                     /protein_id="AAS73174.1"
                     /db_xref="GI:45644928"
                     /translation="MAILVTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRV
                     KEITGSDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTEYYDNNVY
                     GTLVLIEEMISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLMVEKIL
                     HDVSLAYPFFRTTILRYFNPVGAHPSGKIGEDPNGIPNNLMPYICSVASGKYKQLSIY
                     GNDYETKDGTGVRDFIHVMDLANGHIAALKHRDEGSNYKVYNLGTGHGYSVLELLAAF
                     QRITSVNIPYVLVERRAGDIAECWSDPSKAYLELGWKACLGLDEMVRDAWNWQQSNPD
                     GYKFSEKN"
BASE COUNT     3925 a   1710 c   2326 g   4072 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga agatacttgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt gttaatgtcg ataaattgac gtacgccgga aacctggagt cacttgctga
      241 tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgatt ctgttgcaat
      301 ggcgcggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg ctgaaagcca
      361 tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg ttggtactta
      421 tgtccttttg gaagcggctc gcaattactg gtctgctctt gatggcgaca agaaaaatag
      481 cttccgtttt catcatattt ctactgacga agtctatggc gatctgcctc atcctgacga
      541 agtaaataat acagaagaat tacccctctt tactgagacg acagcttacg cgccaagcag
      601 tccttattcc gcatcaaaag catccagcga tcatttagtc cgtgcttgga aacgtaccta
      661 tggtttaccg actattgtga ctaactgttc gaataactac ggtccgtatc atttcccgga
      721 aaaattgatt cccttggtta ttctcaatgc tctggaaggt aaaggattac ctatttatgg
      781 taaaggggat caaattcgcg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc cgaaagagaa
      961 atcttaccgt gagcaaatta cttatgttgc cgatcgcccg ggacacgatc gccgttatgc
     1021 gattgatgct gagaagattg gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag
     1081 cgggattcgg aagacaatta actggtattt gaataataat gattggtgcc gacgagttca
     1141 agatggctct tatcaacgtg agcgtttggg ggtgattaga tgaaaggaat tattttagcc
     1201 ggtggttctg ggactaggct ttatccaatt acaatgggag tttcaaagca gctattaccc
     1261 atttatgata aaccgatgat ttattatcct ttgtcggtgc tgatgttggc aggtatacaa
     1321 gatattctta ttattacaac gccagaagat cagcaagggt tcatgagatt acttggggat
     1381 ggaagccaat ttggtattaa tctgacatat gcaattcaac ctagtccaga tggtttagcc
     1441 caagcattta ttattggtga aaactttatt ggaaatgaca cagtatgtct tatgcttgga
     1501 gataacattt tttttggaca aggttttaca cccaaactcc gggatgcagc agaaagaaca
     1561 tcaggtgcca cagtctttgg atatcaagta atggatcctg agagatttgg agtagtggag
     1621 tttgatagaa attttaaagc tttaagtatt gaagaaaaac ctgccaatcc aaaatcaaat
     1681 tgggctgtca caggattata tttttatgac aataacgtta tcgatattgc aaagtgtatt
     1741 aaaccttcta aacgtggtga attagaaatt acggcagtta atgaagttta tctcagaaat
     1801 aataatttaa ctgttgaatt actaggacgt ggttttgcat ggctggatac aggaacccat
     1861 gacagcttga ttgaagctgg gagttttgta gagacagttc aaaaaaggca agggatgatg
     1921 gttgcttgtc cggaggagat tgcatggcga aatggctggt taaaccatga tgaattacag
     1981 gcaaaaggtg aatttttgaa aaaaaatcac tatggacagt atttactaag gttaattaaa
     2041 tcgcatgaat aataacgtaa aacttataca ctttgataag aaaggtgaca accgaggctt
     2101 tcttgttgtt gttgagcaaa ataaagatat cccaattgaa attaaacgag tttactacat
     2161 gtttgatact ggttctgatg taagacgggg atatcacgca cataaaaaac ttacacaaat
     2221 agcaatccct gttaagggat cttgcaagtt tttgcttgat gacggtaaat cggtggaaca
     2281 tgttacatta gattcaccgt cagtgggatt aattatagaa ccaatgattt ggcatgaaat
     2341 gtacgattat tcatcagatt gtgttttaat ggtgttggca gatgactttt atgatgaaag
     2401 tgattacatt cgtaattatg ctgatttcat ccgggagatg actaatgcgt aatttgtcgc
     2461 tctttacaaa aaatacaaaa ttaattccag ttgaaataaa tgatgcggaa tttatttatt
     2521 cactgcgggt agattctcgt ctcaataagc atatttctac aataaatggg acggtaattg
     2581 aacaggccga atggattgaa gattataaaa aaagagaaga aaataatgaa gagttttatt
     2641 ttataataaa aagaactgat aatgatgaaa aaattggcac gataagactt tataacatca
     2701 cgaaagatga tggtttttgt tgggggagtt ggattctaaa tgatgacaaa atgaaaagct
     2761 ctgcaataga aagtgcctac ctgatataca aatttggttt tttacataaa aactataagt
     2821 attcatattt tcaagttgat aaaaaaaata aagaagtaat cgcatttcat aaaaaaacag
     2881 gagccaccat aataaaagag gatgacgtga attttcactt tgaattttat gtggaaaatt
     2941 ttattcgttt aagagaaaaa tatagtaaat atttttgagg ggaaagaaaa tgattgattt
     3001 tttgaattta aaaagaataa atgctcaata tgaaatggaa ttaaaagatg cttgctccag
     3061 agttattgat tctggttggt atattatggg gacggagtta aagacatttg aggaaaaatt
     3121 tgcaaattgg tgtggagtta agcatgttat tggtgttgcc aatggcttag atgcactaat
     3181 ccttgttctt cgggcttgga aagaaatggg gaaattaaat gacggagatg aaattattgt
     3241 acctgccaat acatatattg catctgtttt ggcaataact gaaaataatc tagtccctgt
     3301 attgactgaa ccagatcctt tgacattcaa cttaagtgcg ggtgggattc gtaaagtaat
     3361 tacagggaaa acaaaagcaa ttttgccagt gcatttgtat ggacaaatat ctccaatggc
     3421 agaaataatg gagattgcgg cagagtttgg tttattggtt ttagaggatt gcgctcaagc
     3481 tcatggttcc tctattgatg gacgcaaagc tggcagttgg ggtcatgcag ctggattcag
     3541 tttctatcca ggaaaaaatc ttggcgcttt aggtgacgca ggtgcgataa caacgagtga
     3601 tgatcaactt gcagatattt tgacagctat tagaaattat ggttctcatg aaaaatataa
     3661 aaacaaatat caaggcttaa atagtcgatt ggatgaaatg caggcggcat ttttgaatgt
     3721 caagttaaaa tatcttgata gggaaaataa attaagacgt gcaatagcta aaaaatatat
     3781 tgaagaaatc aagaatccac taatttcact gcctgtaatt gctggcaatg aaatgtctca
     3841 tgtatggcat ctttttgtta tcagaacttc agagcgtgat tctttgcaaa aacatttgaa
     3901 aactgaaggt attactacat tagtgcatta cccaatagcg cctcataatc aagaagcata
     3961 taaagaatat agttttttat ctctcccttt aactgagaga atacatagag aagtcttatc
     4021 gctgccaatg gatccgacaa tgagcatcga cgatattaat atggtaattg aagcgattaa
     4081 tagatggaaa agctaaattt cgcaaaagtt acgatactca gtggtgtagt aacatgcttt
     4141 agactaattt gtggccttct tatttcaaaa gttgttgcaa tttatactgg tccaattgga
     4201 atagcaaatt tgggacaatt gcaaaatttt gtggccttta taaatggatt catttcatca
     4261 caagtttcac aaggagttaa tagatattca gcagaaaatc aaggtgatta cgaaaatgca
     4321 tgtttttatt ggcgtgcatc aattaaatta tccttcatag cctgttgttt tattataagt
     4381 acgggggtgc ttttttccag taaaatatca gaatacttat tttatcaaag tgatttgtat
     4441 tggttaatta taattgctct atgcgctctt cctcttaata tagtaaacaa tatattttta
     4501 ggggttttaa atggactaaa tgaatataat agatttttcc ttgcgaatgt tttagccata
     4561 tcctcatcgt tgttatctat ggtgggctta gtttattttt ttggcctcaa aggcgcatta
     4621 gtgtctgcat cattaaataa tgcggttgca ggtgtctggc ttattactat tataattaaa
     4681 agaccatggt ttaaatttaa atactgggtg ggacatactc ctcggcataa catcactcag
     4741 atgaaaaatt acttttatat gggggtgatt ggagcgttaa ctggacctat ttcgatgata
     4801 gttgttcgaa ctattctcac taataatttt tctttagagg atgccggata ttggcaagct
     4861 gtaaatagaa tttctgaggc ttatctggct gttcttacta cggcattgac agtttattat
     4921 tttccaaaaa ctgcagccgc aagaagatat agcgaatata taactctttt aaagaccggg
     4981 gcttgtattg ttgtaccgct cgcgttgagc atggctttaa ccatctacgg cttgaaagat
     5041 tttataatta gtatcttgtt cacggctgac tttatacgtg ctcgggagct ttttttattt
     5101 caaaatatcg gggattttct gcggatttgt agttggctgt ttgccactat attgttagca
     5161 aagggatatt ttaaaataaa tgctatattg gaggttatgt tttcaataat ttttccagta
     5221 ttggtaaagt ttttgataat taattttgga ctaaatgccg ttagtattgc ctacagcatc
     5281 acttatgcat tgtattttat agtggtgtgt attatttatt gttggcatct gacaaaaaag
     5341 gccctataac aaatgataat atcttcagat aacctaagtg ttattatccc agtttataat
     5401 gaagagaaga atgttattaa tattcttcaa tctcttgaat gccagtcttt aacgggattt
     5461 aatgttattg tcattgatga tggttcaaaa gatatgactg cttctcttgt aaaagaatat
     5521 aaaccatcat catatagttt aagcttaata caacaaagta atatgggggc tgctcgggca
     5581 agggagaatg ccatcaattt tactcatagt gagtatattg catttataga tagtgatgac
     5641 tctttaagtt cagatgctct tgaaaaagca ttagctccga tgctggataa taaagatatc
     5701 gacatctcac tatttgaact ggtacatatt aaaaacttaa atcatgatac taataacata
     5761 tttgtacctt attctacaac caaattaata tgtggagaag aggcttttgc taattgtatt
     5821 tcatattggg gattgcatgg gtttggtatc tacaaaagaa aattgattca aaaatcctat
     5881 ggtatttatt ataaatataa taaaaacaaa gaaaattata taaataatga tgaggttatt
     5941 tcaagaatca gttttggatt atctaaaaat atctatttat catctggaag atatttcttt
     6001 gtacagaaca tggattcaac aacaagaaga ataaatgaaa gttattataa agttataaat
     6061 aatgctgttt atttaagaga gtatatagat gaagaaataa aatataatga ttttgattgt
     6121 ctaggtgaag ctaataaact ccttgtaagt actatatggg gagtgtttgt tcgttatcaa
     6181 aaatggaaga aaaaattcag tgatgaaaca aacggtaaat ggcgcgaagc aataaaaaaa
     6241 ggaatgcaat atataaaaaa aatcaataac aataagaata ttaacctgca tattaaatct
     6301 aaaatgcaat tatatataat atctaaactt gttagctaaa tatgaaaaat ctattaatta
     6361 caattattgt tgcatcatat aactcaaaag attacatact agagacatta gagtcatgca
     6421 ttaaccaaca atatcaaaat attgagatta ttatagtaga tgattgctct agtgataata
     6481 gtgtagaact tataaaacaa tggtgtaaag aaaagaaaag cacatctcct aaaattagat
     6541 gtatattagt cgaaagcgaa agaaataaag gaattcctgc aaaccttaat aatgctcttc
     6601 cctttattaa tggagactgg attaaatgta ttggttcaga tgatattctt cttcccgaag
     6661 caatctctga gttcgtaaat agattagaaa aatgctcaaa tttggataat ataggtgcag
     6721 tgtttactta tttccaaacc tttggatatt ctgtaaatgt tagtactcga tatccatcct
     6781 catggacaag aactatatgc caaatgccac catcatggtt aaagaaggca gcggctatga
     6841 ttcactttaa taatgtggca ccttgtgctt ttataaataa aaatcatttt agtggatttg
     6901 atacttcata taaactttta gaagatcttc ctctatggct aaaaatgatt aaaaataata
     6961 aaaacacgct attttttgat tatacaactg tattatatcg tctacatgat tctcaaatca
     7021 cctcgcttaa taacattaaa gtcaatgatg ttcttattaa tgatctttgt cgtttaaatt
     7081 attatcgaaa aaataatggt tattatttgg cttaccttca tcataaattc aacctttact
     7141 gcgcagtaaa aaggatgcga ttttacagat acttaaaaat aataaatcct ttgaatttat
     7201 taattgtact atatgaaaga gtaaaaaaat aatgtatata ttatcaatgg ctataccatt
     7261 atttttcata tcgttgtgtt ggcatcgatt tagtcatcaa actcaagttg catttcgttt
     7321 attattcata gtatttttca gtctaagttt cttttcatat tatacaaatg gcgtggattg
     7381 gagtgtttat tatctaagat tcatcgagga tgaggggtta tttacctcgt ttgaatttgg
     7441 gtttgttatt attttaaagc tattgcttat tgtttcagat gataattttg gcttggcaat
     7501 tttactctat tattttctct gttttgtatt gctctcatta atattgaaaa aatacaaagt
     7561 taatgaacca ttatttttgg gatgtctttt attgttattt ggctacaacc tttttttaga
     7621 gcagttacgt caattactgg catgtataat agtattttat gcgatgctat tatacaatca
     7681 aaataaaaat cttatagaat tatgtatatt ggttataaca gcatcaactt ttcatgtgtc
     7741 tgcagtaata attttaccga caatcttttt aatctctttt agaaatgtga ctacttttat
     7801 cataattacg gtatcctcaa ttacaagtat tgtggtattt atgatagcac tttattcagc
     7861 tatatcctct cttgctgcta tgagctttgt ttttgctaaa attgattatt atttgcatca
     7921 atatccagtt gtcctaaatt ttggttggct aaatatactt gatgctttat ttattttatt
     7981 ctacatgcgt tatagaaatg caattgatag acagataaat ataaatatat taacgcgaat
     8041 tattttcgtt ggttctgtta ttcatctgtt cagtggatcg attacttttc tggctcgcgt
     8101 gtgtttttat ttttactttg taggtgttta tatatactgc tattcattaa atgacaagac
     8161 cccgcgggta tttgctatta aaaatttcaa tacgttagta ttggctgcct ttgttaatgt
     8221 agtactttta ttgaactttg catcctattt tagaaatgag caagcccccg ttgggtttta
     8281 taatatgaac tttcaatttt ttaacacatt taatgataat tatcttagaa cgaacgcata
     8341 cgataaatat tttaaaggaa tgcagggcag cgtggattaa ctatgagtaa aagaattatt
     8401 tttgttatta ctgggctggg attaggtggg gctgaaaaac aaatatgttt gttagctgat
     8461 aaattgtatt tgcataataa tgaagttaat attgtcgtgt tgaatggaaa tgtcaatgtt
     8521 actccattga atgagaatat tcgaattcat aatttgcata tgaaaaaaaa tccacttggg
     8581 ttactaaatg cagtgctcag gctcgtgaaa ataattagtt atttcaagcc ggacattgtt
     8641 catggacata tgttccatgc aaatatcgtt gccagacttg caaaagtttt tacctataat
     8701 aaatacaaat taatttgtac agcacatagt aaaaatgaag gtgggcatat taggatgctc
     8761 gtttatagat taactgattt tttatgtgat gctacgacta atgtaagtaa tgaagctctt
     8821 gatgttttta ttaagaaaaa agcctttagc aaatcgaagt caattcccgt ttataatggc
     8881 attgacactg atctatttaa atatgatcct ttacaaagag ataaaataag gcatagttta
     8941 aatatcatta atgatgatag ccttatactt tctgtaggcc gacttactga tgcaaaagat
     9001 tatccaaact tattaaaagc attgttagaa ttacctgaac gatataaatt agtcataata
     9061 ggtgaaggtg aagccagaaa tgaagtcgag cgtacgattt taaattacgg ccttgaaaaa
     9121 agagtgaaat tgctaggtag tattgctaat gtcgccactt attattctgc atgtgatatt
     9181 tatgtatcgt cctctaagtg ggaaggcttt ggtttggttg tagctgaggc tatgtcttgc
     9241 cagcgattgg tcgttgcaac taatgccggt ggggtggctg aagttgttgg tgatatgaac
     9301 tatattgtac ctgtttcaga ccctcagttg ttggcaaata aaattaatga agttatggag
     9361 tttgatcacg aaactaaaat ggaagtaatg tatagaaata gagagtttgt tttaaaaatg
     9421 ttctcaatag atatgatcgt taatcaatgg atgaaattgt attcttcaat ctaatccata
     9481 gcttaatgga aaaatataga atttgataaa ttaatacact ctcatatgtg tattatccgt
     9541 tgcgttatct atagttaaaa ataaaattcg gtgttttttg tttcattgtt tttgaataaa
     9601 tcataattta agttatgatt taaaaaataa gatactgtta tattgtaagg tttttaaata
     9661 tgaaagtgct tttaataggt aaccaatcta gtactattat tttgttcaga aaggctgtta
     9721 ttgaaaattt gatatcaaaa aatattaaag tatatacact tacgatggat aataatgagg
     9781 gtaattttaa taaaattaaa aaaataggtg caatcccttt atcctattct ttttcacgat
     9841 ctggtattaa tcctatatct gattttttta atactttttt tttggcaagg aaaataaaag
     9901 agattaatcc tgattctgtt ctttgctttt tcccaaaacc agtcatatat ggtactcttg
     9961 ccgctatgat tgctggcgta aaaaaacgtt acgctttatt ggaagggttg ggtttttgtt
    10021 tcacacaaaa taaagataaa gactcgttta aaaaaatagt cttaagaaag attcaaggtt
    10081 ttttatataa gatgacatta ccttttgcaa ccaaagttct ttttctcaat aatgatgact
    10141 atcatgactt ggttgttaaa aacaatatta aaatcaaaga ttataaagtt attggtggca
    10201 taggtgtaaa tctcaatgaa tttagttatc atccaccaat ttgtggggag atccattttg
    10261 gtatggtggc aagattattg agagagaagg gcgtatgtga atttgttgaa gcggctaaaa
    10321 taataaagaa aaaatatcca atggtccgat ttagtatagc tggtgatttt gatgataatc
    10381 cgggtggaat tgatgctgca caaattgtaa attggaaagc ggaaggttat gttgaatttt
    10441 taggaccgtg ctcttatata aaagaatatt tgagtgatat cagcgtcttt gtattacctt
    10501 cttatcgaga aggtgtcccc aaaagtactc aggaagcaat ggcaataggt cgtgcgatta
    10561 ttacgaccga tgtgccaggt tgtcgagaaa cagtaattga tggatataat ggatatcttg
    10621 tacctccatg ggatacacaa aaacttgctt tagttatgga aacatttatt catgaacgtg
    10681 agcttattgt cactatggga ttaaatagtc gaaggttcgc attgtcaaaa tttaatgaaa
    10741 gtgaatctgc aaattcttta gttaatatta taactgcata agagttatta aattatccaa
    10801 gctttaataa cattctacct gctagtcttg ttgctttgat taaatgaggg tgctgttgta
    10861 tggctatatt agtgacgggt ggtgctggat atattggctc acatactgta ttaatgctgc
    10921 tgaaagaaca atatgaagta atagtgcttg ataattttca taattcttca gttgaatctt
    10981 tgcatcgcgt taaagaaatt actggtagtg atatctgtat cattaatggt gatattcgag
    11041 atcgtgttat tttaaaatcc atctttactg aaaacagcat aacagatgtt attcattttg
    11101 ctggcctgaa atcagtaagc gattcaattc aattcccaac agagtactac gataataatg
    11161 tatacggtac acttgttctt attgaggaaa tgattagtaa taatatcaag aatctaatat
    11221 tcagctcctc agccactgta tatggtactc cagagaagat acccgtaaaa gaaacattcc
    11281 ctgtaggtgg cactactaat ccctatggga cttcaaaact tatggttgag aaaatattac
    11341 atgatgtttc tttggcatat ccttttttta gaacaacaat tttgcgttat tttaatcctg
    11401 ttggcgcaca tccttccgga aaaattggag aagaccctaa cggtattcca aataatttga
    11461 tgccttatat atgttcagtt gcaagtggta aatataagca attatccatc tatggtaatg
    11521 attatgaaac taaagatggt actggcgtta gagattttat tcatgttatg gatctcgcaa
    11581 acggtcatat agctgccttg aagcaccgag atgaaggtag caattataag gtttacaatt
    11641 taggcacagg gcatggttat tcggttcttg aacttttggc tgcttttcaa cggataacct
    11701 ctgttaatat accttatgtg ttggttgagc gccgagcagg cgacattgct gaatgttggt
    11761 ccgacccgtc taaagcatac cttgaacttg gatggaaagc ctgtttggga ttagatgaga
    11821 tggttcgcga tgcctggaat tggcaacaaa gcaatcctga tggttataaa ttttctgaga
    11881 aaaactgaat tgcttagcct cagtaaaaat ataatacttt tctaacctga gttaccgtaa
    11941 gcttcatata gacctgcgtt aaagttcgca gtaacccctg acaggagtaa acaatgtcaa
    12001 agcaacagat cggcgtcgtc ggtatggcag tga
//
