LOCUS       EU294171               11106 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serotype O105 O antigen gene cluster, complete
            sequence.
ACCESSION   EU294171
VERSION     EU294171.1  GI:168481337
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O105"
                     /db_xref="taxon:562"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24824.1"
                     /db_xref="GI:168481338"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KTLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIAQNYEGRQ"
     misc_feature    108..10793
                     /note="O antigen gene cluster"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24825.1"
                     /db_xref="GI:168481339"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24826.1"
                     /db_xref="GI:168481340"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKKLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3032..3565
                     /gene="rmlC"
     CDS             3032..3565
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24827.1"
                     /db_xref="GI:168481341"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDSFINIQWPYD
                     ECIILSDKDKHQPGLSR"
     gene            3664..4407
                     /gene="wbsV"
     CDS             3664..4407
                     /gene="wbsV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsV"
                     /protein_id="ACA24828.1"
                     /db_xref="GI:168481342"
                     /translation="MCKIVVYTAITGNYDNIKPLSYVNTNFDYLCFTDYEYTGVIPEP
                     WKQIRMPPAKWCNKDLARYIKMNVHEILPNYEASVWIDGNIDIINNIEGLVFDALKKG
                     GASSYQHWGRNNINEEMIECAKIGYDSIFILLKQMKQYGNEGFISNELYETNVLIRDH
                     TNSTISEFSKIWWEQYMQYGKRDQYAFTYAAWKSGLNIHNLGRHDPRFIKKYFSYFPH
                     KNGKSIKLYRIKQLINKITNRITLRIIKI"
     gene            4429..5457
                     /gene="wzy"
     CDS             4429..5457
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACA24829.1"
                     /db_xref="GI:168481343"
                     /translation="MVYGSLWCLCFILSFFNNNSYFKYYITLCIIMVLWFLASFRGAG
                     VDGDYANYIEYINDAQSIGYSYRGGYFFDWIVNFFVYIGLPVTYVFAVYALAIPIKIK
                     LFQKFSLLSSSILLGYVGFFIYLHDFTQIRAGLAVGIAFWGIYYYSYQNKIKAVILFF
                     ASCFIHPSLIFLVAFVYINKFLSNKLLVLTLMISIMLCVTNANTFIVQKIVDLSGSAD
                     LDLYYRLAVDGQIIKPFGAFPLLNLFISIVSLYYLNNIARENALLSIFIKMLLFSQIS
                     WFLFSPIPVLAARISQIFLFSIVFVLPHLSLKLFNQPITIPALYSFVGFLAFIIIAEL
                     MHPYQLGF"
     gene            5465..6307
                     /gene="wbsW"
     CDS             5465..6307
                     /gene="wbsW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsW"
                     /protein_id="ACA24830.1"
                     /db_xref="GI:168481344"
                     /translation="MKITILVVLYNKKFEESTTLQTILSEKHTLKKMNVELCIHDNSP
                     NSQLDECYISEMKEFVKCYYTHTPENMTLREIYNNIINTLKYNTYLCLMDDDTSLPQS
                     FFNEVISAINNNPDIELIVPRVIVNDKLYSPHKSYSFINRPTVRDIKGIQKSKNMSAI
                     NSGMVIKSNFFKRCGFKYPEYTSYYGTDKVFFDHYLKYNQEYYVLDIDINHDVSNHPY
                     NKDDVRYSQIISSVNFFWMQHLKGNYALKFLYIIYIILYRIKLSILRKNLIFIKSIVS
                     LGNK"
     gene            6307..7443
                     /gene="wbsX"
     CDS             6307..7443
                     /gene="wbsX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsX"
                     /protein_id="ACA24831.1"
                     /db_xref="GI:168481345"
                     /translation="MKNNSYKVFPFYFPQFYATKENDMWWGKGFTDWELVKKAQAVHQ
                     SQSQPRIPLNGYYDQSDPVVIKKQSKLASEYGIDGFNFYHYWFDGTVLLDKPMQNLYN
                     DKSIDIEYFFTWANETWTRQWVGRPQDLLIKQEHKVDINIWNEHYEYLRKFFLDDRYL
                     KINNSPVLCIYRPELVKSLSEWVAFFNNKAKCDGFNGIHFIALRAYSIANADSVYKYF
                     DKIVNFQPRFAINTHLKKSSPLKKLIESTLRIAPEWLQLQLVRIIKNKRATYNQYKYS
                     DYIQSMKNDVTEYKGKPVYPVVFPDWDNAPRYKENATFFCESSAYGFEKALNIACDIT
                     RNHDDKLIFINAWNEWSEGAYLEPDEMHKYSSLEIIKKVFQDKR"
     gene            7440..8324
                     /gene="wbsY"
     CDS             7440..8324
                     /gene="wfgK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgK"
                     /protein_id="ACA24832.1"
                     /db_xref="GI:168481346"
                     /translation="MKRIAILLAAYNGARWLEEQISSILQQTNVEVTIYVSIDLSNDE
                     TYLLVSKLALKEPRIIILPYGDIYGGAAKNFYRLICEVDYENYDYIALSDQDDVWRKN
                     KLERACEFLKNNDFYSSNVIAFWENGKQALITKSQDKVAFDHFFESAGPGCTFVFKRE
                     YALQLKRFLMINKHAMDVELHDWLIYAFARENNLKWHIDSYPGMYYRQHKNNQVGANN
                     SLKAIIKRIRLIKSGWYRNEIIKILNLFEQSDIPFRDNLCNKNYFTSLFLLKYIYMIR
                     RRRKDRAFLVLMIILGLF"
     gene            8341..9324
                     /gene="wbsZ"
     CDS             8341..9324
                     /gene="wbsZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsZ"
                     /protein_id="ACA24833.1"
                     /db_xref="GI:168481347"
                     /translation="MNFVSLENMISDIRGFIPELAIHDFDLIVGIPRSGMIPAYTIGL
                     YLNIDVTDVSSFVKNVPLQRGISREKPTSLKLPQDAKKILLVDDSYTTGKSLANTIGK
                     IPMELRNRIVSFALYTTDRENHNLDMFIRVIKYPRVFEWNILNHSIIGNSCLDIDGVL
                     CMDPTDDDNDDGQKYITFLENAKPKFIPKVKIKYLVTNRLEKYRTHTEKWLSKNNVKY
                     AQLIMLDLKTKEERIRSGLHSTHKAKFYKHSGCSLFIESDVKQAYEIMMQSGLPVYCI
                     DDNTMYKPGMTKYFTKQPGNALKKIILWLPILIYKNIPKPYQKIIRRLIKK"
     gene            complement(9366..10793)
                     /gene="wzx"
     CDS             complement(9366..10793)
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACA24834.1"
                     /db_xref="GI:168481348"
                     /translation="MSIFNNLKWNTFSQVFKILTQLISIVYLARIIPPNEYGIMAMAG
                     VVMNFASLFRDLGTSAAIIQKEQLQDETKSAVFWLNFLLGFLVFIIIITLTPFISHLY
                     NQPKLLIVLPLLSFSFIISGSSSLHLAMLERESKFFIISKIEVFSAALSLLLAIILAN
                     NSFGIYSLVFQSLLNSLSSAVLLWIVSPWRPKGKPRHVINHLKEIFRFTSQLSLFNII
                     NFFARNLDSFLIGKYMSASILGSYNLAYRIMLFPLSSLTFIANRSLFPILSQKKNNYK
                     EINDIYLNTIYIIWFITLPLVTVITSLNKLFIQVVFGEQWILSADILFWLAPTAIIQS
                     VLSTTGTIFMSQGRTGILLLLGIQGTILTAVSFMVGIHCGIVDLAKYYFIANAIHFIP
                     CIIFTMKTIKSNVLSIFKKTYPLFISAAISFILISNIRAHFASDIKSLFVLSAIGGAI
                     YLLLLLPTSESRKLIKTLQTKIKKR"
BASE COUNT     3709 a   1663 c   2138 g   3596 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttccgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacttggct gctgaaagcc
      361 atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct catccagatg
      541 aagtaaataa taacgaagaa ttacccttat ttactgagac gacagcttac gcgccaagca
      601 gcccttattc cgcatcaaaa gcatccagcg atcatttagt ccgtgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaactgtt cgaataacta cggtccttat catttcccgg
      721 aaaaattaat tccattggtt attctgaatg ctctggaagg taaaacatta cctatttatg
      781 gtaaagggga tcaaattcgc gactggttgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga
      961 aatcttatcg tgagcaaatc acttatgttg ccgatcgtcc gggacacgat cgccgttatg
     1021 ccattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gcgggattcg taaaacggtg gaatggtacc tggctaatgc aaaatgggtc gaaaatgtga
     1141 aaagtggtgc ctatcagtca tggattgcac agaactatga gggccgccag taatgaatat
     1201 cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     1261 gggtaatttg attgctcttg atgtccactc cactgattat tgtggtgatt ttagtaaccc
     1321 tgaaggtgtg gctgaaacag tcaaaagaat tcgacctgat gttattgtta atgctgcggc
     1381 tcacaccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 tagcgttgaa tcaattgcaa aagcggctaa tgaagttggg gcttgggtaa ttcattactc
     1501 aactgactac gtattccctg gaaatggcga cacgccatgg ctggagacgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agccggagaa aaagcgttac aggaacattg
     1621 cgcgaagcat cttattttcc gtaccagctg ggtatacgca ggtaaaggaa ataacttcgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg cgaagaactg gctgtgataa atgatcaatt
     1741 tggtgcgcca acaggtgctg agctgctggc tgattgtacg gcacatgcta ttcgtgtggc
     1801 actgaataaa ccggaagtcg caggtttgta ccatctggta gccagtggta ccacaacttg
     1861 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggtattc cccttgcact
     1921 caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggtgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga ctatggctgt
     2221 cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc tttctacact
     2281 gatgttagcg ggtattcgcg atattctgat tattagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg gtagccagtg ggggctaaat cttcagtaca aagtgcaacc
     2401 gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatctt ctacggtcat gatctgccga agttaatgga
     2521 tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggcg atcagcctgg aagaaaaacc
     2641 gttacaacca aaaagtaatt atgcggtaac cggactttat ttctatgata acgacgttgt
     2701 cgaaatggcg aaaaaactta agccttctgc ccgtggcgaa ctggaaatta ccgatattaa
     2761 ccgtatctat atggagcagg ggcgtttatc cgttgccatg atgggacgcg gttatgcatg
     2821 gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg caacaattga
     2881 agaacgtcag gggctgaaag tttcctgccc ggaagaaatt gcttatcgta aaggatttat
     2941 tgattctgag caggtgaaag tattagctga accgctgaaa aaaaatgctt atggtcagta
     3001 cctgctaaaa atgattaaag gttattaata aatgaacgta attaaaacag agattccaga
     3061 cgtattaatt ttcgaaccga aagtttttgg tgacgagcgc ggtttctttt tcgagagctt
     3121 taaccagaag gtttttgagg aagctgtagg ccgcaaagtt gaatttgttc aagataacca
     3181 ttcgaagtct tgtaaaggtg ttttacgcgg actgcattat cagttagaac cttatgcaca
     3241 agggaaattg gtgcgctgcg ttgttggtga agtttttgac gtagctgttg atattcggaa
     3301 atcgtcacca acttttggca aatgggttgg ggtgaattta tctgctgaga ataagcgcca
     3361 gttgtggata cctgaaggat tcgcacatgg ttttttggtg ctgagtgaga cggctgagtt
     3421 tttgtataaa acgactaatt attatcatcc tgaaagtgat aggggaatta tttgggatga
     3481 ttccttcata aatatccaat ggccctatga tgagtgcatt attttatctg acaaagataa
     3541 acatcagcct gggttatcta gataattaat ataacctggg ttttttattg ttattcatga
     3601 gctatcttta tgaaatagtt gagtttccca atttagaata gattaatttg tcaaggtcaa
     3661 aatatgtgta aaattgtggt gtatacagct attacaggga attatgataa tattaagccc
     3721 ctgtcatacg ttaataccaa ctttgattat ttgtgtttta cagactatga atatacaggt
     3781 gttattcctg agccttggaa acagattcga atgccaccag cgaagtggtg taataaggac
     3841 ttggcacgat atattaaaat gaatgtgcat gagattttac ctaattatga agctagtgtt
     3901 tggattgatg gaaatataga cattatcaac aatattgagg gtttggtttt tgatgcctta
     3961 aaaaaagggg gggcctcatc ataccagcat tggggccgaa ataatattaa tgaggaaatg
     4021 attgagtgtg caaaaattgg atacgatagt atttttattc ttttgaaaca aatgaaacaa
     4081 tatggtaatg aaggtttcat ctcaaacgaa ctgtatgaaa caaatgtttt gattagagac
     4141 catactaata gcactatttc tgaattctct aaaatttggt gggagcaata tatgcagtat
     4201 ggtaaacgag atcaatacgc ctttacttat gctgcgtgga aatctggact aaatattcat
     4261 aatttaggac gacatgaccc tagatttatt aaaaaatatt tttcatattt tccacataaa
     4321 aatgggaaaa gcataaagct atataggata aaacaattaa ttaataaaat aaccaatcgt
     4381 attacgctca gaattattaa aatataacag tattaaggtt tatttataat ggtttacggt
     4441 tctctatggt gtctatgttt tatattaagt ttttttaata ataacagtta ttttaaatat
     4501 tatattacac tatgtataat aatggttctt tggtttttgg cttccttcag gggggcagga
     4561 gttgatggtg attatgcaaa ttatattgag tacataaatg atgctcaaag tattggatat
     4621 tcttatagag gtggttattt ttttgattgg atagttaatt tttttgttta tattggcttg
     4681 cctgttacat atgtttttgc agtatatgca cttgccatac cgataaaaat aaaattattt
     4741 caaaagtttt cattattatc ttcttctata ttactcggtt atgttggttt ttttatttat
     4801 ctgcatgatt ttactcaaat tagagctggc ttagctgttg gtattgcttt ttgggggatt
     4861 tattattact catatcagaa taaaataaag gcagtaatac tattttttgc gagttgcttt
     4921 attcatccat cgctcatatt tcttgtggcc tttgtataca ttaataaatt cttgtctaat
     4981 aaattattag tgctcacact gatgataagt attatgttat gtgtaacaaa tgcaaataca
     5041 tttatagtac aaaaaatagt tgatttaagt ggcagcgcag atcttgattt atattatcgc
     5101 ttggcagttg atggtcaaat tattaaacca tttggagcat ttccattact gaatttattt
     5161 atatcgatag tatcactgta ttatttgaat aatatagcaa gagaaaatgc tttactttca
     5221 atctttatta agatgctttt gttttcgcaa atttcatggt tccttttctc tcccatacct
     5281 gtattagctg cacgaataag tcagattttt ttattttcaa ttgtctttgt tttgccgcat
     5341 ttatctttaa agctatttaa tcagccaata actataccag cactatatag ctttgtgggc
     5401 tttttagcct ttattattat agccgaatta atgcatccat atcagttagg attttgatac
     5461 ataaatgaag ataactatct tggtagtgct ttataataaa aaatttgaag aaagcactac
     5521 attacagaca atattgagtg aaaaacatac cttaaaaaaa atgaatgttg aactctgtat
     5581 tcatgacaat tcacccaatt cacaattaga tgagtgttat attagtgaga tgaaggaatt
     5641 tgtaaaatgt tattatacac acacaccaga aaatatgaca ttaagagaga tatataataa
     5701 tataattaat accttaaaat acaatactta tctttgcttg atggatgatg atactagttt
     5761 accacagtcc ttctttaatg aggtaataag tgctatcaat aataatccag atattgagtt
     5821 aattgtacca cgagttatcg ttaatgataa gctttatagc ccacataaaa gttattcatt
     5881 tataaataga ccgacagtta gggatattaa aggaatacag aagtccaaga acatgtcggc
     5941 tataaatagt gggatggtaa tcaaatcaaa tttttttaaa cgttgtgggt ttaaatatcc
     6001 tgaatataca agttattatg ggacggataa ggtttttttt gatcattatc taaaatataa
     6061 tcaagaatat tatgttttag atattgatat aaatcatgat gtaagtaacc atccatataa
     6121 taaagatgat gtacgttatt cacagattat ttcatctgtg aatttctttt ggatgcaaca
     6181 tttaaaagga aactatgccc ttaagttttt atacataatt tatattatat tatatcgtat
     6241 caaactatcc atactcagga aaaatttaat ttttattaaa tcaatagtat ctctaggtaa
     6301 taaataatga aaaataatag ctataaagta tttccttttt attttcctca attttatgca
     6361 acgaaagaaa atgatatgtg gtgggggaaa ggttttactg attgggaact agtaaaaaaa
     6421 gctcaagcag tacatcaatc tcagtcacaa cctcgtattc ctctaaatgg ctattatgat
     6481 cagtctgatc cagtagtaat aaaaaaacaa tcaaaattag caagtgaata tggaatagat
     6541 ggttttaatt tctatcacta ctggtttgat ggtactgtgt tgctagataa acctatgcaa
     6601 aatttatata atgataaaag tatcgatata gaatatttct ttacatgggc taatgaaaca
     6661 tggacaaggc agtgggttgg acgcccgcaa gatctactaa taaagcaaga gcataaagtg
     6721 gatataaata tatggaatga acactatgaa tatttgagga aattttttct tgacgataga
     6781 tatttaaaaa tcaataattc acctgtgctt tgcatatata gacccgaact tgtaaaatcg
     6841 ttaagtgaat gggtggcttt ttttaataat aaagcaaaat gtgatggatt taatggcata
     6901 cattttatcg cattacgagc atacagtatt gcaaatgcag attctgttta taaatatttt
     6961 gataaaattg ttaactttca acctagattt gcaataaaca ctcatttaaa aaaatcgagc
     7021 ccactaaaga aattaattga aagcactcta aggattgctc cagagtggct tcagttgcag
     7081 cttgtaagaa taataaaaaa taagagagca acttataatc aatataaata tagtgattat
     7141 atacaatcaa tgaaaaatga cgtaacggaa tataaaggta agcctgtata ccccgttgtt
     7201 tttcctgact gggataatgc cccaagatat aaagaaaatg caactttttt ttgtgaatct
     7261 tcagcatatg gttttgaaaa agcattgaat atcgcttgtg atattacgag aaatcatgat
     7321 gacaagctga tatttattaa tgcttggaat gaatggtctg aaggagcata tttagaaccg
     7381 gatgaaatgc acaaatattc tagtttagaa attattaaaa aagtttttca ggataagaga
     7441 tgaaacgcat tgctatttta ttagcagcat ataatggcgc tcgatggtta gaagagcaaa
     7501 ttagctcaat cctacaacaa acgaatgttg aggttactat atatgttagt attgatctgt
     7561 caaatgatga aacttattta ttagtttcaa agttggcttt aaaggaacct aggatcataa
     7621 ttcttcccta tggtgatata tatggagggg ctgcaaagaa tttttaccga ttaatttgtg
     7681 aagttgacta tgaaaattat gattatatag ctttgtctga tcaggacgac gtgtggcgaa
     7741 aaaacaaact agaacgtgca tgtgagtttt taaaaaataa cgatttttat tctagcaatg
     7801 tcatcgcatt ttgggaaaat gggaaacaag cactaataac taagtcgcaa gataaggttg
     7861 catttgatca tttttttgaa tcggcagggc ctggttgtac atttgttttt aagagagaat
     7921 atgctcttca acttaagcgt ttcttgatga taaataagca tgccatggat gtggaacttc
     7981 atgactggtt gatttacgca tttgcacgag aaaataactt aaaatggcat attgattcgt
     8041 atccgggaat gtattatcga caacataaaa ataaccaggt aggagcaaat aattcactaa
     8101 aagcaattat taaaagaatt cgacttataa agtccggatg gtataggaat gaaataataa
     8161 agatattaaa tttatttgaa caaagcgata ttccctttag agataacctt tgtaataaaa
     8221 attactttac ttcattattt ctcttaaaat atatttatat gataagacga aggagaaaag
     8281 atagagcatt tttagtttta atgattattt tgggtctttt ttaacttata ggaatattca
     8341 atgaattttg tatcgttaga aaacatgatt agcgatatta ggggttttat accagaactt
     8401 gcaattcatg attttgacct cattgtcggt attccaagaa gtgggatgat tccagcatat
     8461 acaattggat tgtatttaaa tattgatgtt acagatgttt cttcttttgt gaaaaatgta
     8521 ccattgcaaa gaggaatttc tagggaaaaa ccaactagcc ttaagctgcc tcaagacgca
     8581 aaaaaaatac tattagttga tgatagttat acaacaggaa aatcgttagc taatactata
     8641 ggcaaaattc ctatggaact taggaatcgc attgtatcat ttgcattgta taccacagac
     8701 agagaaaatc ataatttaga tatgtttata cgggtaataa aatacccacg tgtgttcgaa
     8761 tggaatatcc tgaatcatag cattattggc aatagttgct tagatataga cggtgtgtta
     8821 tgtatggatc ctacagatga tgataatgat gatggacaaa aatatataac tttccttgaa
     8881 aatgcgaaac caaaatttat tccaaaagtg aaaataaagt atcttgttac aaacaggttg
     8941 gaaaaatata gaactcatac tgagaaatgg ttatctaaaa ataatgttaa atatgcgcaa
     9001 ttaataatgt tagatcttaa aacaaaagaa gaaagaataa gatctgggct tcattccaca
     9061 cataaggcta aattttataa acattctggt tgttcacttt ttattgaaag tgatgttaaa
     9121 caagcatatg aaataatgat gcaaagtggt ttgccggtat attgtattga tgacaataca
     9181 atgtataaac ctggtatgac aaaatatttt acaaagcagc ctggtaatgc attaaaaaaa
     9241 atcattctct ggttaccaat actaatatat aaaaatattc ctaagccata tcagaaaatt
     9301 attcggcgtt tgataaaaaa ataaaagaag aaccaagtca tatggcttgg ttctttgctg
     9361 aaaaattacc tttttttaat ttttgtttgc aatgttttaa taagtttcct gctttctgat
     9421 gtgggcaata gaagtagtaa ataaatggct ccaccgattg ctgataaaac aaaaagcgat
     9481 ttaatgtcag atgcgaaatg agctctaata tttgatatta atatgaagct tatagcagca
     9541 gatatgaata gtggataagt ttttttaaat atgcttaaaa catttgattt tattgttttc
     9601 atcgtgaata taatacatgg aataaaatgt attgcatttg caatgaaata atattttgca
     9661 agatcaacaa ttccgcaatg gatgcctacc ataaatgata ccgcagttaa tatagttcct
     9721 tggatgccta aaagaagcaa aatacctgtt ctcccttgag acatgaaaat agtgccagtg
     9781 gtgcttagca ccgactgtat tattgcagtg ggggcaagcc aaaataatat atccgcagac
     9841 agaatccatt gttctccaaa aacaacttga atgaaaagtt tgtttaagct agtaattact
     9901 gtaactaatg gtaaagtaat aaaccaaata atataaatgg tatttaaata aatatcattt
     9961 atttctttgt aattattttt cttctggctt aatataggaa ataaagaacg attagcaata
    10021 aaagtcaaag atgagagtgg gaatagcata atccgataag ctaaattata agatccaagt
    10081 atagatgctg acatatactt accgatcaga aaactatcaa gatttctggc gaaaaaatta
    10141 attatattaa aaagagaaag ttgtgaagta aaacgaaata tttcttttaa atgatttatc
    10201 acatgtctag gtttgccttt tggtcgccat ggggatacta tccataatag tactgcagag
    10261 ctcaaagaat taagcagaga ttgaaatacc aaactatata ttccaaagct attattagct
    10321 aatataatag ctaataaaag agacaaagca gctgaaaata cttcaatttt agaaataata
    10381 aaaaatttcg attctctttc aagcatagct agatgcaatg atgaagagcc ggaaataata
    10441 aagctaaaag atagtaaagg taaaacaatt agtagtttag gttgattgta caaatgtgat
    10501 ataaaaggtg ttagtgttat tataataata aataccaaga agcctaacaa aaaattaagc
    10561 caaaaaacgg cacttttagt ttcatcttgc aattgttctt tttgtataat tgctgctgag
    10621 gtccctaaat ctcgaaataa actagcaaaa ttcattacaa caccagccat tgccatgatg
    10681 ccgtattcat ttggcggtat tattcttgct aaataaacaa tgcttattag ctgagttagt
    10741 attttgaaaa cttgtgaaaa agtattccat tttaaattat tgaaaatgct catattttaa
    10801 tctcactgtt ccgattttta aattatacct gaactattag aatgccacaa tatttttgat
    10861 tgttgatact aatctctata ttattgatat cacattggcg attagtttgg gaattttact
    10921 acaggcgata taagtttata tctatcctgg cttggcgatt tattatttgt tgttaacaga
    10981 atacctttgt aatttatatt aactgtatat atctatttct tttatcgttg aatcaaaatc
    11041 tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgacatgt
    11101 atatat
//
