LOCUS       AB972417               15419 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthesis gene cluster,
            complete sequence, strain: H521a.
ACCESSION   AB972417
VERSION     AB972417.1  GI:733167685
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2014) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H521a"
                     /db_xref="taxon:562"
                     /note="serogroup:O106"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ02183.1"
                     /db_xref="GI:733167686"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHLIERINCINGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1069..1974
                     /gene="galF"
     CDS             1069..1974
                     /gene="galF"
                     /note="O106_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ02184.1"
                     /db_xref="GI:733167687"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGPKFRKSIEKLLGE"
     CDS             2334..3371
                     /note="O106_02"
                     /gene="wbaC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02185.1"
                     /db_xref="GI:733167688"
                     /translation="MIISEINLRANMKVIVNNFFDGILKRGIPIYTSELVQKLRDNNV
                     QVIYVACPKAFHKLPSWFLNVLFIIVEQLLIPIMGIFYRTKYNIYPYNSSSIIDLLLG
                     KAIVIIHDFISLKKNKRNLAAIYVKVCIYFSSFLAKKVIFISQSTQRVSKRISLFKCI
                     ESILLPNPFFSFEKIANVTEIKDLGYLLLVSGLGDNKDLGTALDYYFSIPREERIPLK
                     ILGCGNGISKVNDLIDGRDTDKEIEIIGFVSLEEVVKLYCNSKIVWAHSLAEGYGRTL
                     AEAKLTCKNVICTRISAFREQMDSNIFFYTYYSEYYEVYSYLVDNSPSVERKALHEHI
                     LFENELRKIYE"
     CDS             3364..4566
                     /note="O106_03"
                     /gene="wbaD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02186.1"
                     /db_xref="GI:733167689"
                     /translation="MSNIDINKKQILVLTPRFPFPVIGGDRLRIYKICKELSKHYDLT
                     LLSLCDKREELNYEYDREVFSSVHRVYLSKKKSILNVIFSLFSNTPLQIGYYKSKEFE
                     DKLKQLLPEHSATLSHLIRVGDYVKENKDINFLEMTDAISLNYKRVKEKASLLSLKTF
                     VYSFEQKRLERYERTINNKFSLTTLVSQVDSDYLYPDRPNNVLVCGNGVDAVSLPFSE
                     RKIAKDKKITLVFIGNLYSLQNMDGVRWFTKEVLPFLNKHGNFEFKVIGRITDKDKSW
                     LESQPGVVVTGEVDSITYAAADGHIGVCPIRLGAGIQNKVLEYMALGLPCISSTVGFE
                     GLGAEEGKEIYVANTKEEYLRVLNYFITNLDKYTETALVAKKFIGENFSWEAKLSPYI
                     QKIKESVK"
     gene            4568..5983
                     /gene="manC"
     CDS             4568..5983
                     /gene="manC"
                     /note="O106_04"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAQ02187.1"
                     /db_xref="GI:733167690"
                     /translation="MIIPIIMAGGSGTRLWPLSRSLYPKQFLSLINENSLLQETLKRL
                     DGLNCLPPVIVSNNEHRFIVAEQLRQSGVDDFQIILEPVGRNTAPAVALAALKSLELH
                     GDHHMLVLAADHAIQDIEAFHAAVLAAEQESVDNKLVTFGIVPTKPETGYGYIKKGEQ
                     VKNSVFKVNSFVEKPDLETAKNYLEQKCYLWNSGMFMFKASVYLDELKKFRPDILAAC
                     KESLSSASTDLDFIRLNSDVFAECPDESIDYAVMEKTQDCVVIPLDADWSDIGSWTSL
                     WEISEKDEHENVSHGDVINYNSRNNYIYSEGSLISTVGVNNLIIVQTKDALLVAQQDN
                     VQDIKKIVEILKKQKRSEHISHREVYRPWGRYDSVERGDRYQVKRITVKPGECLSTQM
                     HHHRAEHWVVVAGTAKVTCGERTFFVTENESTFIPIGTVHTLENPGKIPLEVIEIQSG
                     VYLGDDDIVRLSDKYGRVEDK"
     gene            5988..7409
                     /gene="manB"
     CDS             5988..7409
                     /gene="manB"
                     /note="O106_05"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAQ02188.1"
                     /db_xref="GI:733167691"
                     /translation="MNITNDVIKNSGIAFGTSGARGLVKDFTPNVCAAFTYAFIDVMM
                     KDFSFKEVALAIDNRPSSYAMAQACAAALLECSIKPIYYGVIPTPALANQAIADGIPA
                     IMVTGSHIPFDRNGLKFYRPDGEISKENENSIIHAEKEYPDVTVLPELQCSKRAADTY
                     IERNTSIFSNIFKGKRIGIYEHSSAGRDLYSEIFSKLGAEVVSIGRSDEFVPIDTEAV
                     SKEDEEKALHWSSEYDLDMIFSTDGDGDRPLVADENGVWLRGDILGLFCSKAMNIEAV
                     AVPVSCNTVIQTCGWFNHVTLTKIGSPYVISAFDSLNNSFDRVAGFEANGGYLLGSDV
                     NYNSGMIKALPTRDAVLPALMVLALAIKNNVTISKLLAELPPRFTYSNRVQNFATSKS
                     KSIIASAQENPQEFIERIGFVDLHVQSVDTIDGLRLTLSDGSIIHLRPSGNAPELRCY
                     AEADNIIKAEKLVLEVLHKVTLL"
     gene            7593..8924
                     /gene="wzy"
     CDS             7593..8924
                     /gene="wzy"
                     /note="O106_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ02189.1"
                     /db_xref="GI:733167692"
                     /translation="MHKQYYSLLCNKIQFKHLAIYFSVFLSLSSCFLWGGADTFVSQV
                     YIDILATISTALLVVLMFYYYINRVRYLSLTLLFAFVFSLIIGLPSIYLYYFKNANHG
                     FEIICIWGMLINSILYLQTTETVIKEEKRYLTSLFYILFSIVALCQLYKILIYLMFIL
                     NSGEGHLAIYTESEELLSQVPFFIRAISGFAVVMSLSTFYFKTPTYIKVIAFVLLASD
                     LAIGIRNKFFFSVICIFILYMYSNEYRVRNLFKQLSKPIFLIAGFLCFSMVSYFREGY
                     QIDFMIYIGIVLDSLSSTLGGMQNLFDMQSSGWDQLHPAVVFTQVLPLSGLGFLDDQQ
                     IAKDFSIIVLGDVSSGIALSSSGLLESSLLSLNLGVIIYLLYLSGVMLFIKWGLNNQR
                     IIINFVAISMISGFFYAVRGELILPLAFLLKSLPIVLISPLLVKNKIYHGQ"
     gene            8966..10201
                     /gene="wzx"
     CDS             8966..10201
                     /gene="wzx"
                     /note="O106_07"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ02190.1"
                     /db_xref="GI:733167693"
                     /translation="MIKEIFSDGIFSLFNKICYFGFKTLAIILITRSLGEDVGGEFIF
                     LVGFLEVLRVVTDFGVDLFVIKKYNDSTDKIKLLNVTFAQKILSGVIVLLLFCLYSFV
                     QGYQYNVYIPIVLALPVALFFNFSNSYFQSLNSNKSLTFPIAIASIATCTIFAIQYFL
                     NVKFNAWDYLCVELLFVSLVTLKLFVSVKFRFIRTSAFDVLMSILSLYKYTLSIGLTA
                     VVVIIYSRIDNFYLKTFAPGDLAAYGQIFRMVDPLVMVSSVFSTVAYARFCRINLQEK
                     NAFTKVFQFLLCIVAYGVLSSLVYFFIIKYLGHFVLISNVNNDFLIMMFLIVACVKCI
                     NGGTTAIIQSQGFYKVGLYVSYVCILTSIPTMYFLIMKYGVKGAAMTILLVECISFLL
                     LLCSFFMIKKMRPKKTFEM"
     gene            10354..11760
                     /gene="gnd"
     CDS             10354..11760
                     /gene="gnd"
                     /note="O106_08"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ02191.1"
                     /db_xref="GI:733167694"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVVA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            12011..13177
                     /gene="ugd"
     CDS             12011..13177
                     /gene="ugd"
                     /note="O106_09"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ02192.1"
                     /db_xref="GI:733167695"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQHFLQSDKIHFNATLDKNEAYRDAYYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADTILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKRQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            13287..14300
                     /gene="wzz"
     CDS             13287..14300
                     /gene="wzz"
                     /note="O106_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ02193.1"
                     /db_xref="GI:733167696"
                     /translation="MRTWKFPSVRVMMRVENNNVSGQNHDPEQIDLIDLIVQLWRGKM
                     TIIISVIVAIALAIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSD
                     LQETLIGRFSSAFSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKL
                     AQYIQQVDDKVNQELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQY
                     ANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDI
                     ESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNY
                     NAK"
     gene            complement(14483..15094)
                     /gene="hisI"
     CDS             complement(14483..15094)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ02194.1"
                     /db_xref="GI:733167697"
                     /translation="MLTEQLRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTDHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
BASE COUNT     4410 a   2883 c   3357 g   4769 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagataa tcggtgccaa catcaccaat
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tatctgacca tctcatcgag cgcatcaact gtattaacgg caaaatcaac
      181 tggggcatcg gcataggcct tgcgggaagt acttatgata ataactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca actgatccat
      301 gttgaaaatg gtaaacattt tgttatccgt aatatcaaag ctcgtaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagttgcta tttatggttg tgacaacttt
      421 gtgattgata atattgaaat gattaatagt gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcagaat ttccgagtga atgatattca actggataat
      541 actcatcttg cttataaatt gcgcggcatc caaatctccg ccgggaatgc tgtctccttt
      601 gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
      661 catcttttta tgcgaaatat caatgtgatg caggaatcct cagttggacc cgcattgagc
      721 atgaactttg acatgcgcaa agacgttcga ggcgtgttta tggcgaaaaa agaaacactg
      781 ctgtctcttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc agaacggagg
      901 gagtagattt gcgaccattc ctggaaaaat ggagtcatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcaccag taattcagta atcaattcag ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacgaatt taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc
     1141 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
     1321 gtgaagcgtc aactgctggc ggaagtgcaa tctatctgcc cgccgggcgt gactattatg
     1381 aacgtgcgtc agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc
     1441 attggtgaca acccatttgt cgtggtactg ccagacgttg tgatcgacga cgccagtgcc
     1501 gacccgctac gctataacct tgctgccatg attgcgcgct tcaatgaaac gggccgtagc
     1561 caggtgctgg caaaacgtat gccgggcgat ctctctgaat actccgtcat ccagactaaa
     1621 gagccgctgg accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat
     1681 cagccgcaga cgctggactc agacatcatg gccgtgggcc gttatgtgct ttctgccgat
     1741 atttggccgg aacttgaacg cacgcagcct ggtgcatggg gacgtattca gctgactgat
     1801 gccattgctg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac cggcgacagc
     1861 tacgactgcg gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actgcgcaac
     1921 ctgaaggaag ggccaaagtt ccgtaagagc attgagaagt tgctcgggga atagatccaa
     1981 gccaatgtaa caataattta gataaatagc ggcagctaac atcccggcgt ctatgcttta
     2041 aacaaaggaa tgttactgtc gtttttcttt ttaaaacaaa gaataacaac gagttaacct
     2101 actaagttta atctattttt tgttggattt atccttgttt ctgacagcat ttggtaagac
     2161 aattggcgtt tgaatttaag gtagctttgc gatagctagc ggggcggggt acatctttgg
     2221 cattttgcta gtgcactggt agctgttaag ccaggggcgg tagcgtggta agattgtgtt
     2281 tatatgatgc gttctcaaaa taagaaataa taatcctgac tatttttata ctattgataa
     2341 tatctgaaat aaacttaaga gcaaacatga aagtaattgt aaataatttt tttgatggta
     2401 ttttgaaaag aggaataccg atctatactt ctgaacttgt gcaaaagtta agagacaata
     2461 atgttcaggt tatttatgtt gcatgtccaa aagcattcca taagctgcct tcatggtttc
     2521 tgaatgttct ttttataata gtagagcagc ttttaatacc aattatgggt atattttata
     2581 gaactaagta taatatatat ccctataata gtagttccat cattgacttg ttattaggta
     2641 aggcaattgt tataattcat gacttcatta gtcttaagaa aaataaacga aatcttgctg
     2701 ccatttatgt gaaagtctgt atttattttt catctttttt agcaaagaaa gtaatattta
     2761 tttctcaaag cactcagcga gtatctaaga gaatatcgct atttaagtgt atagaatcta
     2821 tcctgctccc aaatccattt ttctcatttg agaaaattgc taatgttacc gaaataaaag
     2881 atcttggata tttacttcta gtttcaggtt taggggataa taaggatcta ggcacagctc
     2941 tggactatta tttttcaatc ccaagggaag aaagaatacc attaaaaata ttaggttgtg
     3001 ggaatggtat tagcaaggtt aacgacttaa ttgatgggcg ggatacagat aaagaaattg
     3061 aaattattgg ttttgtttct ttagaggaag tcgtcaaact atattgtaat tctaaaattg
     3121 tttgggctca ttcgcttgcc gaggggtatg gaaggacttt agctgaagca aaactcacat
     3181 gcaaaaatgt tatatgcaca cggatctctg ctttccgcga acaaatggat tcgaatattt
     3241 tcttttatac ttactattct gagtattatg aagtctattc atacttggta gataattcac
     3301 ccagtgtcga aaggaaagct ctgcatgaac atattttatt tgaaaatgaa ttgagaaaga
     3361 tttatgagta atattgatat aaataagaaa cagatattag ttttgactcc acggttcccg
     3421 tttcctgtca ttggtgggga tcgattacgt atctataaga tctgtaaaga actgtctaag
     3481 cattatgatc ttacgttgct gagtctgtgt gataaacgcg aagagctcaa ctatgaatat
     3541 gatcgtgaag tttttagtag tgttcacaga gtatatcttt caaagaaaaa atctatttta
     3601 aatgtcatat tctctttgtt ttcaaatact cccctacaga ttgggtatta taaatctaaa
     3661 gaatttgaag ataaacttaa gcagttatta cctgaacatt ctgcaactct ttcacatttg
     3721 ataagggttg gtgattatgt gaaagagaat aaagatataa attttttaga gatgaccgat
     3781 gccatttcac ttaattataa acgagtaaag gaaaaagcat ctttattgag tttaaaaaca
     3841 tttgtatatt cttttgaaca aaaaagactc gaacgatatg agcgaactat aaataacaag
     3901 ttcagcttga ctactttggt atcacaggtt gactccgatt atttatatcc agaccgtccg
     3961 aataatgtat tagtctgtgg aaatggtgtt gatgccgtta gcttaccttt ttcagaacga
     4021 aaaatagcta aagataaaaa gataaccctt gtatttatag gtaatttata ttctttgcaa
     4081 aatatggatg gtgtgagatg gttcaccaag gaagttttac catttcttaa caagcatggt
     4141 aattttgaat ttaaggtcat cggacgtatt acagataaag ataagagttg gttagaaagc
     4201 cagcctggcg ttgttgtcac aggtgaagtt gatagcataa cttatgcagc agctgatggg
     4261 catattggtg tatgtcctat tagattaggg gcaggtatcc aaaataaggt acttgaatat
     4321 atggcgttgg gattaccttg tatctcttct actgtaggct ttgaaggctt gggagcagag
     4381 gagggtaagg agatttatgt tgccaataca aaagaagaat atttacgtgt attaaattat
     4441 tttataacaa atctggataa atataccgaa acagcattag ttgcgaaaaa gtttatcggt
     4501 gagaattttt catgggaagc taaattgagc ccatatatcc agaaaattaa ggagtctgtt
     4561 aaataaaatg attatcccaa ttattatggc cggtggttct ggtactcgct tatggcctct
     4621 ttcgcgttcg ttatacccga aacaattttt atcattgatt aatgaaaata gtttgctcca
     4681 ggaaacgttg aagagacttg atggattaaa ttgtttgcct cctgtcattg ttagcaataa
     4741 tgagcataga tttattgttg ctgaacaatt gcgacagtcc ggtgtggatg acttccagat
     4801 tattctagaa cctgtaggta gaaatactgc gcccgccgtt gcgctggctg ctctaaagtc
     4861 tctagaactc catggcgatc atcatatgct ggttcttgct gctgaccatg ctattcagga
     4921 tattgaagcc ttccatgcag ctgttttagc tgcagaacaa gagtccgttg acaataagtt
     4981 agtgactttt ggcatcgtcc cgaccaaacc tgaaactggg tatggctata ttaaaaaggg
     5041 cgaacaagtt aaaaactctg tatttaaagt aaattctttc gtcgaaaaac ctgatttaga
     5101 aacggctaaa aattatctcg aacaaaaatg ctatttgtgg aatagtggta tgttcatgtt
     5161 caaggcgagc gtatatttag atgaattgaa aaaatttcgc cctgatattt tagcagcgtg
     5221 taaagagtcg ctttccagtg ctagtactga tctagatttt atcagattaa attctgatgt
     5281 ctttgcagaa tgtcccgatg agtcaattga ttatgcggta atggaaaaaa ctcaggattg
     5341 tgtagttatt ccacttgatg ctgactggag tgacatcggt tcatggacat cactttggga
     5401 aatatcagag aaagatgaac atgaaaacgt atcgcatggc gatgtaatta actataacag
     5461 caggaacaac tacatttact ctgaaggttc actgatcagc actgtaggag ttaataacct
     5521 tattatagtt caaactaaag atgcgctcct ggtagcacag caagacaacg tacaagacat
     5581 aaagaagatt gtcgaaattc ttaaaaagca aaaacgtagc gaacatatat cccatcgcga
     5641 agtctatcgt ccttggggac gttatgattc tgtagaacgt ggcgaccgtt atcaggtaaa
     5701 acgtattacc gttaagcctg gtgaatgtct ctcaacacaa atgcatcatc atcgagctga
     5761 gcattgggtg gttgtcgctg gtactgcaaa ggtgacttgc ggcgaacgca cttttttcgt
     5821 cactgaaaat gagtcaactt tcataccaat tggcacagtt cataccttgg aaaatccagg
     5881 aaagatacca ttagaagtaa ttgagattca gtcgggtgta tatctaggtg atgatgatat
     5941 tgtgcgccta agtgataaat atggtcgtgt tgaggataaa taattatatg aatattacca
     6001 atgatgtaat taaaaatagc ggaattgcat ttggtacaag tggtgctcgt ggattagtga
     6061 aagattttac acccaacgtg tgtgctgcat tcacttatgc ttttatcgat gtaatgatga
     6121 aggatttttc ttttaaagag gttgcgctcg caatagataa tcgcccaagc agctatgcta
     6181 tggcacaagc ttgcgcagca gcattgttgg aatgtagtat taaacctatt tactacggtg
     6241 taattccaac tccagctttg gcaaatcaag cgattgccga tgggattcct gcaataatgg
     6301 ttactggaag tcatattcca tttgatagaa atggtttgaa attctatcgt ccagacgggg
     6361 aaatttcaaa agaaaatgag aacagcatta tacatgctga aaaagagtat cctgatgtaa
     6421 ctgtattgcc agaattacaa tgttcaaagc gtgccgctga tacttatatc gagagaaaca
     6481 cttcaatttt ttcaaatata tttaaaggta aacgtatcgg gatttatgaa cattcaagtg
     6541 cgggacggga tctttattca gaaatattca gtaagcttgg tgctgaagtt gtaagtattg
     6601 gtagaagtga tgagtttgtg cctattgata ctgaagcagt atcaaaagaa gatgaagaaa
     6661 aagctttaca ctggtcatct gagtatgacc tcgatatgat tttttctacc gatggtgatg
     6721 gcgatcgtcc tttggttgct gacgaaaatg gcgtttggtt acggggtgat atattaggac
     6781 tattttgctc aaaggcaatg aatattgaag cggtagctgt acctgtaagt tgtaatacgg
     6841 ttattcagac ttgcgggtgg tttaatcatg taacgctgac taaaattggt tcaccatacg
     6901 tcatttcagc gtttgattct ctgaataata gttttgatag ggttgcaggg tttgaggcta
     6961 acggtgggta tcttttaggt agcgatgtaa attataacag cggtatgatt aaagcgcttc
     7021 caacaagaga tgctgttttg cctgctttaa tggtactggc actggctata aaaaataatg
     7081 tgactatttc aaaactcttg gcggagttgc caccacgatt tacctactca aatagagttc
     7141 aaaattttgc aacaagcaaa agcaaaagca ttatcgcgtc agctcaagaa aatcctcaag
     7201 aatttatcga aagaataggc tttgtcgatc ttcatgtaca atcggtggat acaattgacg
     7261 ggttaagatt gacactaagt gatggttcaa taatccattt gcgaccgtca ggcaacgcac
     7321 ctgaattgcg atgctatgca gaagcagata acataataaa agctgaaaaa ttagtgttag
     7381 aagttttgca taaagttact cttttgtaaa aaaatataaa cttttttcta ctcttgagcg
     7441 gtttgtcatt taaccgctca gtaatagatt gtttataaat tgtttatatt tagcattggt
     7501 atttttattt gcgttcatcc cgaataagag attgtaaaat taactgtttc ttatttttca
     7561 aatgagttac ttcactagta aagctaatta ctatgcataa gcaatattat tcattattgt
     7621 gcaataaaat acagtttaaa catctggcta tatatttttc tgtatttctt tcactatcct
     7681 catgtttctt gtggggaggg gcggatacat ttgtatcgca agtgtatatt gatattttag
     7741 ccactatatc tacagcgcta ttagttgttt tgatgttcta ttattatatt aatagagttc
     7801 gctatttatc actaacctta ctttttgcat tcgttttttc tttgattatt ggtttgccat
     7861 cgatttatct ttactatttt aaaaacgcaa atcatgggtt tgaaataatt tgtatctggg
     7921 ggatgttgat caattccatc ctttacttgc aaacaacaga gactgtcata aaagaagaaa
     7981 aaagatattt aactagttta ttttatattt tgttttctat tgttgcactg tgtcaattat
     8041 acaaaatatt aatttatctt atgtttatat tgaattcagg agaagggcat ttagcaattt
     8101 atactgaaag cgaagaattg ctatcccaag ttccattttt tattcgtgct attagtggtt
     8161 ttgcagttgt aatgtcattg tccacttttt attttaaaac accgacctac attaaagtta
     8221 ttgcatttgt tttacttgca tctgatttag ctattggaat acgtaataag tttttctttt
     8281 cagtgatttg tatttttata ctatatatgt actctaatga gtatagagta agaaatttat
     8341 ttaagcagct ctcaaaacca atatttctca tcgccggttt tttatgcttc tcgatggtat
     8401 cgtatttccg tgaagggtac caaatcgatt ttatgattta tattggcatt gttttagatt
     8461 cgctatcctc tactcttggg gggatgcaga atttgtttga tatgcaatca tcaggatggg
     8521 atcaacttca tcccgctgtg gtattcacac aagtattacc gctaagcggt cttggttttc
     8581 ttgatgatca acaaatcgca aaagattttt caataattgt tttaggtgat gtatcctctg
     8641 gtattgcgtt gtcatcttca ggattgttag agtcatcgtt gctgagtcta aacttgggag
     8701 tgattattta tttactgtat ttatcagggg tgatgctatt tattaaatgg ggactaaata
     8761 atcaacggat aataataaac tttgttgcca tctccatgat atctggattt ttttatgctg
     8821 ttcgtggtga actgattttg ccattggctt ttcttctaaa atcattgccg attgttctta
     8881 tatctccatt acttgttaaa aataaaattt atcatggtca gtaagtggtt tatttttcga
     8941 ccaggatctc accagtaggc atgacatgat caaagaaatt ttctccgatg gcattttttc
     9001 gctatttaat aaaatatgtt acttcggttt taaaacactt gcaataattc tgattactcg
     9061 ttccttagga gaagatgtag gcggagagtt cattttttta gttggttttc tcgaagtcct
     9121 tcgggtggtt actgattttg gtgtggatct gtttgttata aaaaaatata atgattctac
     9181 agataaaata aaactattaa atgtaacgtt tgcacaaaaa attctttctg gagtaattgt
     9241 tttactactg ttttgtttgt attcttttgt tcagggttat caatataatg tctatattcc
     9301 tattgtgtta gccttacctg ttgctttatt ttttaatttc tcaaatagct attttcaatc
     9361 tctcaattcc aataaatctc tcacgtttcc gatcgcaatt gcatctattg caacgtgtac
     9421 gatcttcgct attcagtatt ttttaaacgt taaatttaac gcatgggatt acctgtgtgt
     9481 tgaactatta ttcgtatcgc tggttacact aaaactattc gttagtgtca agtttaggtt
     9541 tattagaacg tcagcttttg atgtgctaat gtcaattctt tctttatata aatatacatt
     9601 aagtataggt cttacagctg ttgttgtaat tatttatagc cgtatagata atttttatct
     9661 caagacattt gccccagggg atcttgcagc atatggccag atatttagga tggttgatcc
     9721 actggttatg gtttcttctg tattttctac agttgcctat gcaagatttt gccgaattaa
     9781 cttacaagaa aaaaatgctt ttactaaggt gtttcagttc ctgttatgta ttgtggcata
     9841 tggcgtgctg agttcattag tttatttctt tataataaaa tacttgggtc attttgtatt
     9901 gatttctaat gttaataatg atttcttgat aatgatgttc ttgattgttg catgtgtgaa
     9961 atgtatcaat ggtggtacaa ctgcaataat tcagtcacag gggttttata aagtgggttt
    10021 gtatgtttcg tatgtttgca ttttgacatc gataccaact atgtatttct taataatgaa
    10081 gtacggtgtt aaaggtgctg ctatgacgat tctacttgta gagtgtatta gtttcttatt
    10141 acttctctgc agctttttta tgataaaaaa gatgcgtcct aaaaaaacat tcgaaatgta
    10201 atcaatgagt cctctttgaa tagaattagt ttgttctatt tatgaacact tagcttgagt
    10261 aagttctaac atagattact aattgtttga tactatctgc tttacaagca taaccgcgta
    10321 tgtcgcggtg accacacctg acaggagtat gtaatgtcca agcaacagat cggcgtcgtc
    10381 ggtatggcag tgatggggcg caacctggcg ctcaacatcg aaagccgtgg ttataccgtc
    10441 tccgtcttca accgctcccg cgaaaaaact gaagaagttg ttgccgagaa cccgggtaag
    10501 aaactggttc cttattacac ggtgaaagag tttgtcgagt ctcttgaaac ccctcgtcgc
    10561 atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc cctcaaacca
    10621 tatctcgata aaggtgacat catcattgat ggtggtaaca ccttcttcca ggacactatt
    10681 cgtcgtaatc gtgagctttc agcagagggc tttaacttca tcggtaccgg tgtttccggc
    10741 ggtgaagagg gggcgttgaa agggccttcc atcatgcctg gtgggcagaa agaagcctat
    10801 gaactggttg caccaattct gaccaaaatc gccgcagtag ctgaagacgg tgagccatgc
    10861 gttacctata ttggtgccga tggcgcaggt cactatgtga agatggttca caacggtatt
    10921 gaatacggcg atatgcaact gattgctgaa gcctattctc tgcttaaagg tggcctgaac
    10981 ctcaccaacg aagaactggc gcagaccttt accgagtgga ataacggtga actgagcagc
    11041 tacctgatcg acatcactaa agacatcttc actaaaaaag atgaagacgg taactacctg
    11101 gttgatgtga ttctggatga agcagcaaac aaaggtaccg gtaaatggac tagccagagc
    11161 gcgctggatc tcggcgaacc gctgtcgctg attaccgagt ctgtgtttgc acgttatatc
    11221 tcttctctga aagatcagcg cgttgccgcg tctaaagttc tcactggccc gaaagcacag
    11281 ccagcaggcg ataaggctga gtttatcgag aaagttcgcc gtgcgctgta tctgggtaaa
    11341 atcgtttctt acgctcaggg cttctctcag ctgcgtgcgg cgtctgaaga gtacaactgg
    11401 gatctgaatt acggcgaaat cgcgaagatt ttccgtgctg gctgcatcat ccgtgcacag
    11461 ttcctgcaga aaatcaccga tgcttatgcc gaaaatccgc agatcgctaa cctgctgctg
    11521 gctccgtact tcaagcaaat tgccgatgac taccagcagg cgctgcgtga tgtcgttgct
    11581 tatgcagtac agaacggtat cccggttccg accttcgcgg ctgcggttgc ctattatgac
    11641 agctaccgcg ccgcagttct gcctgcaaac ctgatccagg cacagcgtga ctatttcggt
    11701 gcgcatactt ataagcgcat tgataaagaa ggtgtattcc atactgaatg gctggattaa
    11761 tctgatttaa ccaacaataa aattgaggcc cggcgtatat tgcaccgggc tttttttttt
    11821 gccaattatc ttatagacta aatttcactg cttaatatta acttaataaa tatcagctat
    11881 tcttataaag aaaatctgaa ttgtttttcg ttgcgttgac catcgaacaa cgtagcgtta
    11941 aaacttttag ctcttatcag gatgctaaaa acatcatgat tcacagttaa gttaattctg
    12001 agagcatgaa atgaaaatca ccatttccgg tactggctat gtaggcttgt caaacgggct
    12061 tctaatcgca caaaatcatg aggttgtggc attagatatt ttaccgtcac gcgttgctat
    12121 gctgaatgat cggatatctc ctattgttga taaggaaatt cagcattttt tgcaatcaga
    12181 taaaatacac tttaatgcca cattagataa aaatgaagcc taccgggatg cttattatgt
    12241 catcatcgcc actccaaccg actatgatcc taaaactaat tatttcaata catccagtgt
    12301 agaatcagta attaaggacg tagtagagat aaatccttat gcggttatgg tcatcaaatc
    12361 aacggtaccc gttggcttta ccgcagcgat gcataagaaa tatcgtactg aaaatattat
    12421 tttctctccg gaatttctcc gtgagggtaa agccctttac gataatctcc atccttcacg
    12481 tattgtcatc ggtgagcgtt cagaacgcgc agaacgtttt gctgcgttgt tacaggaagg
    12541 cgcgattaag caaaatatcc cgaccctgtt taccgactcc actgaagcag aagcgattaa
    12601 acttttcgct aatacctatc tggcgatgcg cgtagcatac tttaatgaac tggatagcta
    12661 tgcagaaagt ttaggtctga atactcgcca gattatcgaa ggcgtttgtc tcgacccacg
    12721 tattggcaac cattacaaca atccgtcgtt tggttatggt ggttattgtc tgccgaaaga
    12781 taccaagcag ttactggcga actaccagtc tgtgccgaat aacctgatct cggcaattgt
    12841 cgatgctaac cgcacgcgta aagattttat tgccgatacc attttgtcac gcaagccgca
    12901 agtggtgggt atttatcgtc tgattatgaa gagcggttca gacaacttcc gcgcgtcttc
    12961 cattcagggg attatgaaac gtatcaaggc gaaaggcgtt gaagtgatca tttacgagcc
    13021 ggtgatgaaa gaagactcat tcttcaactc tcgcctggaa cgcgatctcg ccaccttcaa
    13081 acgacaagct gacgtcatta tttccaaccg tatggcagaa gagcttaagg atgtggcaga
    13141 taaggtctac acccgcgatc tctttggcag cgactaacat cttgttatca gggctattta
    13201 cgccctgatt gtcttttgtt acttacgcaa caattcatta tttttatcac ttatcctata
    13261 gcattcacga ggattatcgc taaactatgc ggacttggaa atttccgtca gttagggtaa
    13321 tgatgagagt agaaaataat aatgtttctg ggcaaaacca tgacccggaa cagattgatt
    13381 tgattgattt aatagtgcag ttgtggcgtg gcaagatgac aatcatcatc tctgtcattg
    13441 ttgctattgc cctggctata gggtatctgg ccgttgccaa agaaaagtgg acttccacag
    13501 ctatcattac tcaacctgat gtggggcaaa tcgctggcta taacaatgcc atgaatgtta
    13561 tctatggtca ggctgcaccg aaagtgtcag atttgcagga gacgttgatt ggtcgcttca
    13621 gttcagcctt ctctgcatta gcagaaacgc tggataatca ggaagaacca gaaaaactta
    13681 ccatcgaacc ttctgttaag aaccagcaat taccattgac tgtttcttat gttgggcaaa
    13741 ctgcagaggg cgcacaaatg aagttggccc aatacattca gcaagttgac gataaagtga
    13801 atcaagagct agaaaaggat ctcaaagaca acattgctct gggacggaaa aacttgcagg
    13861 actctttaag aacgcaggaa gtggtcgcgc aggagcagaa agatctgcgt atccgtcaga
    13921 ttcaggaagc attgcagtat gcgaatcagg cgcaggtgac aaaaccacag gttcagcaga
    13981 ctgaagatgt gacgcaagat acgttgttcc ttcttgggag cgaagcactg gagtcgatga
    14041 ttaagcatga ggcgacccgt ccgttggtgt tctcaccaaa ctactatcag actcgtcaaa
    14101 acctgctgga tatcgaaagc ttaaaggttg atgatcttga tattcatgct taccgttatg
    14161 tgatgaaacc gacgttacct attcgtcgcg atagcccgaa aaaggcaatt accttgattc
    14221 tggcagtgct actgggtggc atggttggcg cggggattgt gctggggcgt aacgctctac
    14281 gcaattacaa cgcgaaataa tctttgcagt tttaaagaaa atgggcaggg tggtgacacc
    14341 ttgcccgttt tttttgccgg atgcgatgct ggcgcatctt atccagccaa aatgtgcaga
    14401 gcgaatagta ggcccgataa gcttgcgcat cgggcaatgg ctacggatgc gactacaaca
    14461 tcgcatccgg tcagtccgca actcactgat gccgtttgcg caggttctca atcaccgtcg
    14521 ttaaatccag cccctgatcc tgcaacagca ccagcaggtg atacatcaag tcagatgcct
    14581 cgttggtcag ctcaaagcgg tcatgtaccg ttgctgccag cgcagtttcc acgccttctt
    14641 cgcccacttt ctgcgcaatg cgcttggtgc cgctggcata cagtttggcg gtataggagg
    14701 tttccgggtc ggcagatttg cgctcggcga gcagttgttc cagttgatac aggaacagcc
    14761 actggtgatc ggtgttgccg aagcagctgc tggtgccttt gtggcaggtc gggccgatgg
    14821 gattcgccag cactaataaa gtgtcgttgt cgcagtccgg ggcaatactc accacattga
    14881 ggaaattgcc cgacgtttcg cctttggtcc acagtcgctg tttagtgcgc gagaagaagg
    14941 tgactttgcc gctttcgatg gttttgtcta aggcttccgg gttcatatag cccagcatta
    15001 gcacttcgcc agataccgcg tgttgcacaa tcaccggcat cagtccgtcg gttttttccc
    15061 agtccagttc gcgacgtagt tgttctgtta acatatcctg atctccacgc cctgtgttgc
    15121 caggtacgtt tttaattcac caatattgat aatttgtttg tggaatacgg aagctgccag
    15181 cgcaccgtca acgtcggcat cgcggaaggc ttcgaggaag tgttccatgg tgcccgcgcc
    15241 accggaggca atcagcggga cgtggcaaac ttcacgcact tttttcagct gctcgaggtc
    15301 gtaaccgtta cgcacaccgt cctgattcat catgttgagg acaatttctc cggcaccgcg
    15361 tttttgcact tcctgtaccc agtcgagcgt ttcccactga gtgacgcggg tgcggcttt
//
