LOCUS       HM485572               16597 bp    DNA     linear   BCT 10-JAN-2011
DEFINITION  Escherichia coli serovar O109 O-antigen gene cluster, complete
            sequence.
ACCESSION   HM485572
VERSION     HM485572.1  GI:313747655
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16597)
  AUTHORS   Perepelov,A.V., Ni,Z., Wang,Q., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Wang,L. and Knirel,Y.A.
  TITLE     Structure and gene cluster of the O-antigen of Escherichia coli
            O109; chemical and genetic evidences of the presence of L-RhaN3N
            derivatives in the O-antigens of E. coli O109 and O119
  JOURNAL   FEMS Immunol. Med. Microbiol. 61 (1), 47-53 (2011)
   PUBMED   20964722
REFERENCE   2  (bases 1 to 16597)
  AUTHORS   Perepelov,A.V., Wang,Q., Ni,Z., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Wang,L. and Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin,
            Tianjin 300457, P.R.China
FEATURES             Location/Qualifiers
     source          1..16597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serovar="O109"
                     /db_xref="taxon:562"
     gene            1137..2207
                     /gene="rmlB"
     CDS             1137..2207
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADR74235.1"
                     /db_xref="GI:313747656"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDTAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTQGIVGETYNIGGHNEKNLDVVYTICDLL
                     DEILPKEGSYRDQITYVTDRLGHDRRYAINADKISQTLGWKPQETFESGIRKTVEWYL
                     NNTNWCRRVQDGSYRRERLGLE"
     misc_feature    1137..15060
                     /note="O-antigen gene cluster"
     gene            2209..3081
                     /gene="rmlA"
     CDS             2209..3081
                     /gene="rmlA"
                     /note="D-glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADR74236.1"
                     /db_xref="GI:313747657"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIKEILIITTPEDQEGFIRLLGDGSQFGIELKYKIQPSPDGLAQAFILGEEFIGNDSV
                     CLVLGDNIFFGQSFSAKLRAVAERAEGATVFGYQVMDPERFGVVEFDDNYKAISIEEK
                     PEIPLSNWAVTGLYFYDNSVVEIAKTIKPSKRGELEITTINECYLKMGKLNVELLGRG
                     FAWLDTGTYDSLIEAGSFVETVQKRQGLMVACPEEIAWRNGWLSNNELHQRGALLSKN
                     NYGQYLLKLSKHER"
     gene            3181..4401
                     /gene="wzy"
     CDS             3181..4401
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADR74237.1"
                     /db_xref="GI:313747658"
                     /translation="MGCTTNQNLIYKSRVSVGSVIVLLVFCIYLFMPPMIMVLLSQSS
                     LSAGMMVVATLLHISFAHYKILNYKILTAVLLILIYGTVSTFYSPDMKPLFSAIALIY
                     IYTATVKFLKYLQEIHDLDAKKAISWLYYIMIIIGIMGVVFNIRFGNYAVKNYPVFPF
                     AEPSHFALAYAPIAICYQLYYCSNKFHSFRTIGISLLLGVLYPNLILVIIGCLQFLIL
                     PKRYSVPLLFILIASVAMYIIKAGMQAIDYFYYRIFFDYSNLNLSNLVYLQGWEAAWI
                     ALKETRLFGLGFQLNGTQSAGFFTDLINSINKSELNRLDGGFLASKLISETGIIGIFF
                     VLFLIFKAVKIIFINKKYQFSFYNKLYFSIMVSLLVELIFRGYGYFSPTFILALSFLS
                     IPNTVCRNEITTHE"
     gene            4394..5530
                     /gene="weiM"
     CDS             4394..5530
                     /gene="wbwG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwG"
                     /protein_id="ADR74238.1"
                     /db_xref="GI:313747659"
                     /translation="MNKKKTKVVISGVNIVEGGALTTFQSIIGAFSKFEDISLVCLVN
                     SKKHFCNYNISDIEFIEYPLIKKRWLRRIKFEYYDCRRISKEIKPDIWISMHDMTPTV
                     ICKNQFVYCHNPSPFYQSKLFDLKMDPSFFIFTLLYKYLYKINIRKNKAVIVQQDWLA
                     HAFKKWYGVKNILVSKPEENDINNISLGCSEQKSYGTYFYPALARTFKNFEVIGEAVQ
                     MLSTRNPQAYAKLNIILTIDGEENKYANYIYNKYSHLKAINFVGRLTRNEVNEYYSKV
                     DVLLFPSKLETWGLPISEAKMKHLPMLVADLSYAKETVGTYDKVTFIDINDPNALAEL
                     FERCITGQHRFEKCSYVPNEDYIHSNSWSELAQNILNKACSKLI"
     gene            5546..6709
                     /gene="weiN"
     CDS             5546..6709
                     /gene="weiN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiN"
                     /protein_id="ADR74239.1"
                     /db_xref="GI:313747660"
                     /translation="MAFLSIVIPTRNRPQYLARVVELIASNTCESEVIVADNSDNDSL
                     KDKLKKYTESGLVKYYYTSENLSVVDNFEKALEKVTGKYIIFLGDDDGIGPQIENIAH
                     WMDDNHVDAAFSYSDKFLVNYYWPGVKSKFFDDKYSSRIFISKFDGSVQFIDSISARS
                     EVRDAPGTGLNNLPRIYHGLVSFNLIAKIKAKGNLFGGISPDIFSAYQIATEAKKIAK
                     INYPFVIPGGCKESTAGQRAAKSDNGSLAETEHTARFGNSLVWDSRIPQYYSPYNVWA
                     YSLLKAVERTDGKVNDFNFYRLYVISYLRYKNFRSYTLSALSEAYKKPSLAGFIKALF
                     AEFNFFVNRVTNKILKNKKESIAASDIIKAYEVLDSFINKNNIKIKYPSQQGN"
     gene            6714..7502
                     /gene="weiO"
     CDS             6714..7502
                     /gene="weiO"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiO"
                     /protein_id="ADR74240.1"
                     /db_xref="GI:313747661"
                     /translation="MIELSECTEISKEFEKIAWNEFFVKRNRGISLQYHYPFLYNKNK
                     NYVNIIIKISGVTVGGLIPQFKYIDNIVYGSIGLVCIKDEYRGLGLFNKLFIYTLAYA
                     KEKYIHKLILWTSKHSLYEKFGFRKKIQEYLYIEPTCSEMMSFEKYENCFTELSETNK
                     PPFVKEFIRVQSGNHIAYLFQDANGISVYEYKGKVENVLDMIKAFLSKPLRILLSKND
                     DLFQLILKEVTSAKLKEDDTEMWYTVDKKIVDVKTPHIAYIDRI"
     gene            7512..8600
                     /gene="weiP"
     CDS             7512..8600
                     /gene="weiP"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiP"
                     /protein_id="ADR74241.1"
                     /db_xref="GI:313747662"
                     /translation="MLPLVKVGMPDKDILIPRIEEVLYSGFIAEGEAVYEFERRFGEH
                     FSLGDNVLGMYSGTAALHTALILAGVKEGDEVITTSMTAEPTNLSILQAGGKPVFADV
                     DVFSGNPSPESIKEKITSKTKAILVVHYAGIPVRLKEISKLAKENGLKLIEDCAHALG
                     SRYDGLGIGQFGDYSIFSLQAIKHMTTVDGGVLYIKDNEQIKRAKKIRWFGMEKGVAR
                     TEVDITELGYKYNMHNVAACIGLAQLQCVQERIQRHIDNGRYFDSVITKIPGLDVPKF
                     DAVASPSYWLYTIFSDDSDDIQRKLDEVGVMASKLHKPNHTHAIFSNENIKLPNLEHY
                     YEKMIHIPCGWWVDNEAREMIVEALKKG"
     gene            8606..9745
                     /gene="weiQ"
     CDS             8606..9745
                     /gene="weiQ"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiQ"
                     /protein_id="ADR74242.1"
                     /db_xref="GI:313747663"
                     /translation="MAISLFNAYYDKSMGDLTNEILASGQIANGKYVALFSESFAKKN
                     GNSHIVTTDNMTNAMELALKMSGVGPGDEVLTSPFSCMASNSPIATLGATPVWVDLAP
                     GSTCINIDEVEKQITKRTKALILYHLAGYANLAESAVALCKKYGIKLIEDCNNSYFTK
                     IGNRNVGTYGDFSVYSFYPNRLINTIDGGAISVRSVEDYVLAKKMTKYGLDPQTFRDS
                     TGEINPQSDIPHIRRTIGMNNLSSALGYSQLSSVDARIQRIKINVSLLYEYFSERDDV
                     HIVKSSDNSNNVNWVFFIQVNKRNELLKTLKLNGIFASKLHQLNSCYTGFKSKEMAIP
                     NAVRLQNTLIALPCGWWLDENQIHHMIDVINDSLGLVESGRWIDD"
     gene            9738..11102
                     /gene="wzx"
     CDS             9738..11102
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADR74243.1"
                     /db_xref="GI:313747664"
                     /translation="MINEKLRKLFGLGLLFSSKLGGLVVALFLVPIYNHYMSNEAFGF
                     VTLFLTLQSLVLLLDCGVTIILSRHTALNVCKKNKISNNQWYNTEVFLTILYLLFGFV
                     FFIFKWLGVNWLSLISYTDLLMIIVALWATVLQNICLTVLLAGKYFSLSSVLLISGNL
                     LKAGGAALIVKYFSPTIGAYVATQALLGISLLFVTRFFCDYFLFFKDVKRKIDFNVIK
                     KIIHEGRALVIFNISAAVLLNLDKIIVSHNISLIALAPYFLASSLCITPISALSGPVM
                     QFFQPGLTKAIFNDSPNVIKTLRVYISTLCVSTLLPSYILWVYRDSLIALWLGNTELV
                     NIVADYTKILLPGIAIGGLGYISYVVLIAIEDFKFQSFLSLSMTAIVVILVVFFSMRQ
                     DIRSICYVYAFYHSLSTLLTWARCYYLPRTQKYALETAKMLFIIMIALAVLCVFFNLI
                     INRI"
     gene            11105..12115
                     /gene="weiS"
     CDS             11105..12115
                     /gene="weiS"
                     /note="dehydratase/epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiS"
                     /protein_id="ADR74244.1"
                     /db_xref="GI:313747665"
                     /translation="MFKNKRLMITGGTGSFGNTVLKRFLKTDVKEIIVFSRDEKKQED
                     MRIALGDSKVKFYIGDTRCYTSIEQAMRGVDYVFHAAALKQVPSCEFYPMEAIKTNIL
                     GTENVLNAAIANNVKRVVLLSTDKAVYPINAMGMSKAMAEKVLIAKSRMIDADKGPVL
                     CATRYGNVMASRGSVIPLFIDKIKNNQELTITDPKMTRFLMSLEDSVDLVLHAFENGQ
                     QGDIFVQKAPASTIEDLAQAIKGLFNSEASNKIIGTRHGEKLYETLVSREEMVKAQDM
                     GKYYRIPADNRDLNYDKYILDGKVEANNIDDYTSHNTTRLSVKEVTELLLTLDYVKEQ
                     LN"
     gene            12155..12700
                     /gene="rmlC"
     CDS             12155..12700
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-xylo-4-hexulose 3, 5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADR74245.1"
                     /db_xref="GI:313747666"
                     /translation="MKITKTHLLGCIVIEPKVFRDARGFFVETYQKEKYQEIGITEDF
                     VQDNRSRSAKGVLRGLHFQTNQPQGKLVSVTSGAVYDVAVDLRPESATFGSYFGLVLS
                     AELNNQLYIPPGFAHGFCVLSDSADFLYKCTQYYNPASESGIQWNDPTLKINWPISNP
                     FISEKDSTLPSFKDYMETYAQ"
     gene            12697..13509
                     /gene="tll"
     CDS             12697..13509
                     /gene="tll"
                     /note="dehydratase/epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Tll"
                     /protein_id="ADR74246.1"
                     /db_xref="GI:313747667"
                     /translation="MKYTVIGGSGFIGSAMCNYLKNNNKNVFIPERDYHFSAGEDLGI
                     VFYCAGYGDCINNYDNVIDANVLLLRKILNNVKFKKLVYVSSTRVYMNENCAKENADL
                     LICHNDNRKLFNLTKLAAEEICRLSKRNVIIVRPSNVYGLALNSKLFLPQIIKNAILN
                     NKIDMYVAPEYSKDYVSVDDLVHIMFELAHKEFNDVQTFNIASGVNTSAAQIADIIKR
                     KTNCEVIWHDVNIQEKFPVTDITKINELVAHNYRDVISDLSKMIDVFYNELH"
     gene            13527..14507
                     /gene="weiT"
     CDS             13527..14507
                     /gene="wfaE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaE"
                     /protein_id="ADR74247.1"
                     /db_xref="GI:313747668"
                     /translation="MKKILFICPSFFKYELEIYKELSLNYEVEYWDERPKNSIIFKVL
                     LRLKFRPLIKKAINQYYINLLNNINGKHFDYVFVINPEALDYNTMNKLKQKCLGVNKQ
                     CYFVLYLWDSLKNKPNAPKLFPLFDKVSTFDRKDSDDFNINLIPLFYTRKFDAKYYPV
                     QPLKYDVCFIGTAHSNRLKYLDEILKIISNNKQISKYIFLFFPSRMLFLIKKLFCNSS
                     TKHKIYFESLPSAKVVDAILSSAVVIDIHHPSQHGLTMRTIEAVGLNKKIITTNADIK
                     KYDFYDPKDVYVYQSGDSKLPDVFLSTSTTNFKNREMYSLKCWVENNLKK"
     gene            14527..15060
                     /gene="weiU"
     CDS             14527..15060
                     /gene="weiU"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiU"
                     /protein_id="ADR74248.1"
                     /db_xref="GI:313747669"
                     /translation="MAKLKMDILRYKLLCGDETNLKYRDYLKIFSPRLFCVFLYRTSS
                     FFYQKRMKLLSKFFSLINFYYSKLKFHLYAPLVEGLFLPHPISIVIGANSIGENCTIF
                     QNVTIGAKTLDIVYNRNVRPLIGNNVTISAGAVVVGPVAINDNVFLGANCVVVNSIES
                     GVIVGGVPAKILGKLTL"
BASE COUNT     5196 a   2700 c   3349 g   5352 t
ORIGIN
        1 attgtggctg cagggatcaa agaaattctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcggcacttc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtaca gtctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttgtgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgt ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtta ttcagaccaa agaaccgctg
      421 gatcgcgaag gtaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccacaa
      481 acgctggatt cagacatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcattt gtgaagtatg gactgcgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccga
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaaattttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtagacatgc
     1021 atgtagtgct ctggtagctg taaagccagg ggcggtagcg tgtattaaca cctctatcaa
     1081 tcaacctaag agccgcttat ttcacagcat gctctgaagt aatatggaat aaataagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgata
     1321 cagctgcaat ggcgcggatt tttgctctgc atcagccgga tgcagtgatg cacttggctg
     1381 ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggc gatctgcctc
     1561 atcctgacga agtaaataat acagaagaac tacccttatt tactgagacg acagcttacg
     1621 cgccaagcag cccatattct gcttctaaag catccagcga tcatttagtc cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac ggcccttatc
     1741 actttccgga aaaattgatt ccgctggtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat catgctcgag
     1861 cattgtatac cgtggtgact caggggatag taggtgaaac ctacaatatc ggtggtcata
     1921 acgaaaaaaa cctggacgtg gtatatacta tttgcgatct tcttgatgaa attttaccga
     1981 aggaaggatc ttatcgcgat caaattactt acgtgactga ccgcctaggt catgaccgtc
     2041 gttatgccat caatgccgat aagattagtc agactttagg ctggaagcca caagaaacgt
     2101 ttgaaagtgg gatccgtaaa acggtggaat ggtatttaaa caatactaat tggtgtcgtc
     2161 gtgttcagga cggtagttat agacgtgaac gtttaggatt agaataatat gaaaggaatt
     2221 atccttgccg gtggttcagg tacgagactt tatccaataa ccatgggggt ctcgaaacaa
     2281 cttctcccta tttatgacaa accaatgatc tattatcctt tgtcagtatt aatgctggca
     2341 gggatcaaag aaatattaat tataacaaca cccgaagatc aggaaggatt tattcgtctt
     2401 ttaggtgatg gaagtcaatt tggaattgag ttgaaatata aaattcaacc tagtcctgac
     2461 gggcttgcac aggcgtttat tttaggggaa gagtttattg gcaatgattc agtatgctta
     2521 gttctcggcg ataacatctt ctttggacag agctttagcg cgaagttaag agctgtagct
     2581 gagcgagcag aaggtgcaac cgtttttgga tatcaggtaa tggatccaga gagatttggt
     2641 gttgtggaat ttgatgataa ttacaaagct atatctattg aagaaaaacc tgaaatcccc
     2701 ctttctaatt gggcggtaac tggactatat ttttacgata attcagtagt tgagatagca
     2761 aaaactataa agccttctaa acgcggagaa ctggaaataa cgaccatcaa cgagtgttat
     2821 ttaaaaatgg ggaaactaaa tgtagaatta ctggggcggg gattcgcgtg gttagatact
     2881 ggcacttatg atagcttaat tgaagcgggc agttttgttg aaacagtgca gaaaagacag
     2941 ggtttgatgg tagcatgccc ggaagaaatt gcatggcgaa acggttggtt gagtaataat
     3001 gaattacatc agcgaggtgc acttttgagt aaaaataatt atggtcaata tcttttaaaa
     3061 ttatcaaaac acgaaagatg atcttcaaaa atggctgtgt tagtatttaa gttagaccat
     3121 atataatcta agataaatat tcacattaca gccatttcca cttcttctag gttgagtaaa
     3181 atgggatgta caacgaatca aaatttgatt tataaaagtc gtgttagtgt tggtagcgtg
     3241 attgtacttt tggtgttttg catatatcta ttcatgcctc caatgattat ggtacttctt
     3301 tctcaatcat cattgtcagc agggatgatg gtagtcgcta cgctattaca tatttcgttc
     3361 gctcactata aaatattaaa ttataaaata ttaactgctg tgttgttaat actcatttat
     3421 gggactgtct caacatttta tagccccgat atgaaaccac ttttctctgc gatagcttta
     3481 atatatattt atactgccac agtaaagttt ttgaaatatt tacaagagat acacgattta
     3541 gatgcaaaaa aagcaatatc atggctctat tatataatga ttatcattgg gataatgggg
     3601 gtggttttta atataagatt tgggaattat gctgttaaaa actatcccgt ttttccattt
     3661 gcagagccaa gtcactttgc tcttgcatat gcgcccatag caatatgtta ccaattatat
     3721 tattgttcaa ataagttcca cagttttcgt acgattggaa taagtttatt gttaggtgtc
     3781 ttgtacccca atttaatact agttatcatt ggttgtttac aatttttgat cttacctaaa
     3841 agatattcgg taccactgct ttttatttta attgcatctg tggcaatgta tataataaag
     3901 gcaggtatgc aagctattga ctatttttat tacaggattt tttttgatta ttcaaactta
     3961 aacctttcaa acttagtata tctgcaagga tgggaagctg cttggattgc cttaaaagag
     4021 acccggctat ttggattggg ttttcagcta aatggtacac aaagtgctgg tttttttact
     4081 gacctgataa actccattaa taaaagtgag ttgaaccgcc ttgatggtgg attccttgct
     4141 tccaaactta taagtgagac aggaataata ggtatattct ttgtgctatt cttaattttc
     4201 aaggcagtga aaataatttt tataaataaa aaataccaat ttagtttcta taacaaactc
     4261 tatttttcga ttatggttag cttacttgtt gagctaatct ttaggggata tggatatttc
     4321 tctcctacat ttattttggc gctatctttt ctttcaatac ctaacactgt atgtcgaaac
     4381 gagataacta cacatgaata agaagaaaac aaaagtggta atatctggtg tgaatattgt
     4441 tgagggtggg gcattaacca cattccaaag tataataggt gcttttagca aatttgaaga
     4501 tatatcatta gtatgtttag ttaactcaaa aaaacatttt tgtaactata atattagtga
     4561 tattgagttt atagaatatc cattaataaa aaaacgctgg ctgcgtagga taaaatttga
     4621 atattatgat tgtaggagaa taagtaaaga aatcaagcct gatatttgga tttcaatgca
     4681 tgacatgacc ccaacagtta tatgtaaaaa tcaatttgtc tattgccata atccttcacc
     4741 attttatcag agtaaactct ttgatttaaa aatggatcct tcttttttta tattcactct
     4801 tctgtataaa tatctgtata aaataaatat cagaaaaaat aaagcagtga ttgtccagca
     4861 agattggcta gcccatgctt ttaaaaaatg gtacggcgta aaaaatattc tggtttcaaa
     4921 acctgaagaa aacgatataa ataatataag tttaggctgt tcagagcaaa aatcatatgg
     4981 cacctatttt tatcctgcac tagccaggac attcaaaaat tttgaagtga taggggaggc
     5041 cgttcaaatg ttaagtactc gaaaccctca ggcttatgct aagttaaata tcatacttac
     5101 aattgatggc gaagaaaaca aatatgcaaa ctatatttat aataaatact ctcatcttaa
     5161 agctataaat tttgttgggc gattaacacg taatgaagtc aatgagtatt attctaaagt
     5221 tgatgtttta ctatttccct ctaaattaga aacgtggggg ctaccgattt ctgaggcgaa
     5281 aatgaaacat ttgccaatgt tagttgctga cttatcatat gcgaaagaga cagtaggaac
     5341 atatgataaa gtaacattta ttgatattaa cgacccaaac gcattggcag agttgtttga
     5401 gcgatgcatt actgggcaac atcgttttga aaagtgcagt tatgtgccga atgaagacta
     5461 tattcatagt aatagttggt ctgaacttgc tcaaaatatt ttaaataaag catgttcaaa
     5521 attaatttaa gaaggtgctg aaattatggc ttttttatca atagtaattc caacccgcaa
     5581 cagacctcag tatttggcaa gggttgtgga gttaatagca tcaaacacat gcgaaagtga
     5641 agttattgtt gcagataata gcgataatga ttcattgaaa gataaattga aaaaatacac
     5701 tgaatctggt ttggtaaaat attattatac atctgaaaat ttaagtgttg tagataattt
     5761 tgaaaaggca ttagaaaaag taactgggaa atatattatt tttctaggag atgatgatgg
     5821 gattggtcct caaatagaaa acattgctca ttggatggac gacaatcatg ttgatgctgc
     5881 attttcatat tcagataaat tcttggtaaa ttattattgg ccgggtgtta aatctaagtt
     5941 ttttgatgat aaatactctt cgcgcatttt tatttcaaaa tttgatggct ctgtacaatt
     6001 tatcgactcg ataagtgcga gaagtgaagt tagagatgct ccgggaacag gacttaataa
     6061 cttacccaga atttatcatg gtttggtttc ttttaacttg atagcaaaaa taaaagctaa
     6121 aggtaatctc ttcggtggaa ttagtcctga tatatttagt gcttatcaaa ttgccacaga
     6181 agcaaaaaaa attgccaaaa ttaattatcc attcgtgata ccaggcggat gcaaagaaag
     6241 tactgctggc caaagagctg caaaatctga taatggaagt ctggctgaaa ccgaacatac
     6301 agcacgtttt ggtaattcac ttgtatggga ttcaagaatc cctcagtatt attctcccta
     6361 taatgtttgg gcatattcat tgctaaaagc tgttgagcgc actgatggaa aagttaatga
     6421 cttcaatttc tatcgccttt acgtaatatc atatcttcgt tataagaatt tcagatcata
     6481 tacattaagt gcattatctg aggcctataa aaaaccttca ttggcaggct tcattaaggc
     6541 actttttgct gagtttaatt tttttgttaa tcgtgtaacg aataaaatat tgaaaaataa
     6601 aaaggaatcg atagctgcga gtgatatcat taaagcatat gaagtgttag attctttcat
     6661 taataaaaat aatatcaaaa taaaatatcc tagtcagcaa ggaaattaat gtaatgattg
     6721 aattatctga atgtactgag ataagcaaag aatttgaaaa gattgcatgg aatgaatttt
     6781 ttgtaaaacg aaatagaggg atatctctcc aataccacta tcctttttta tataataaaa
     6841 ataaaaatta cgtcaacatt ataataaaaa tatcaggtgt tacagtagga gggttaatac
     6901 ctcagtttaa atatatcgac aacattgttt atggttctat aggtttagta tgtattaaag
     6961 atgagtatcg aggcttaggg ttatttaaca agttatttat atatactctt gcatatgcaa
     7021 aagagaaata tattcataag ttaattcttt ggacttcgaa gcatagttta tatgagaaat
     7081 ttgggtttcg taaaaaaatt caagaatatt tatatattga acccacttgt tcagaaatga
     7141 tgagtttcga aaaatatgaa aattgtttta ccgaattaag tgaaactaat aaacctccat
     7201 ttgtaaaaga atttattcgt gttcaaagcg gaaatcatat cgcttatctt tttcaagatg
     7261 ctaatggcat ttcggtatat gaatacaaag ggaaggtaga aaacgtttta gatatgatta
     7321 aagcattttt atctaagccg ctaagaattt tattgtcaaa aaatgatgac ttgtttcaat
     7381 taattttaaa agaagttaca agtgctaaac tcaaagaaga tgacactgaa atgtggtaca
     7441 cagttgacaa aaaaattgtt gatgttaaaa cacctcatat agcatatatt gacagaatat
     7501 aaggaataag catgttacct ttagttaaag ttggaatgcc agataaagat attttaattc
     7561 ctcgaattga agaggttctt tatagtgggt ttattgctga gggggaagct gtatatgaat
     7621 ttgaaaggcg tttcggagag catttttcac taggggacaa tgtgcttgga atgtacagcg
     7681 gaacagctgc gttacatacg gctttgattc ttgcaggtgt aaaagaaggt gatgaggtta
     7741 ttactaccag tatgactgct gagccaacaa atttaagtat acttcaggct ggtggaaaac
     7801 ctgtgtttgc cgatgttgat gtcttctcgg gtaatccttc tcctgaatca attaaggaaa
     7861 agatcactag taagacaaaa gctatccttg tagtgcatta tgctggtatc ccggttagat
     7921 tgaaagagat ttctaaatta gcaaaagaga atggcttaaa attaattgag gattgtgccc
     7981 atgccctcgg gtccagatat gatggattag gaataggcca gtttggagat tatagtatat
     8041 tttctcttca ggctatcaaa catatgacaa ctgttgatgg tggagttctc tacattaaag
     8101 acaacgagca gattaaaaga gcaaaaaaaa ttagatggtt tggtatggag aaaggagttg
     8161 cccgaactga ggttgatatt actgaactag gttataaata taatatgcat aatgttgcag
     8221 cctgtattgg acttgcacaa ttgcaatgcg ttcaggaaag aatacagcgt catatagata
     8281 atggtagata ttttgattcc gtaattacaa aaattcctgg gctcgatgtt ccaaaatttg
     8341 atgctgtagc ttccccatca tactggttat atacaatttt ttcagatgat tctgatgata
     8401 ttcagcgcaa actagatgaa gtaggggtaa tggcttcaaa attacataaa ccaaaccata
     8461 ctcatgctat attttcaaat gaaaatatta agttaccaaa tcttgagcat tactacgaaa
     8521 aaatgattca tataccttgt ggttggtggg ttgataatga agctcgtgag atgattgttg
     8581 aagccttaaa aaagggttga tgtaaatggc aatttcatta ttcaatgctt actacgacaa
     8641 aagtatgggg gatttgacta acgaaattct agcgagtggt caaattgcta atggtaaata
     8701 cgttgcctta ttttcagaaa gctttgccaa aaaaaatgga aatagtcata tcgttactac
     8761 ggataatatg acaaatgcta tggagttagc cctaaaaatg agtggggttg gacctggtga
     8821 tgaagtttta acatcgcctt tttcttgtat ggcctctaat tcacctattg ctacactggg
     8881 agccacacct gtttgggtag atcttgctcc aggatctact tgtatcaata tagatgaagt
     8941 tgaaaagcag atcaccaaaa gaactaaggc attgattttg tatcatttgg ctggttatgc
     9001 taatttagcc gaatcagctg tagcattatg taaaaaatat ggaataaagc ttattgaaga
     9061 ttgtaataac tcctatttta caaaaatagg caataggaat gttgggactt atggggattt
     9121 ttctgtttat tctttttatc cgaatcgatt aattaatact atcgatggtg gtgcaatttc
     9181 tgtccgaagt gtagaagact atgttttggc aaaaaaaatg actaagtatg gtcttgatcc
     9241 ccaaactttt cgagactcca caggagaaat caatcctcaa agtgacatac ctcatatacg
     9301 gcggacgatc ggaatgaata atttatcatc cgcacttggt tatagtcagc tttcttccgt
     9361 tgatgcaaga atacagcgca ttaaaataaa cgtctcatta ctttatgaat atttctcaga
     9421 gagagatgat gttcatattg ttaaatcttc agataattct aataatgtga actgggtatt
     9481 ttttattcaa gtaaataaac gtaatgaatt gctgaaaact cttaaattaa acggaatatt
     9541 tgcttcaaaa ctgcatcagt tgaatagctg ttatacgggc tttaagtcaa aagaaatggc
     9601 cattcccaat gcagtaaggt tgcaaaatac gctaatagct ttaccttgtg gatggtggct
     9661 tgatgaaaat cagattcatc atatgattga tgtcattaat gatagtttag gtttggtaga
     9721 atctggtaga tggattgatg attaatgaaa agctaaggaa attgtttggg ttagggctac
     9781 ttttctcaag taaattaggc ggactggttg tcgccttatt tttggtgccc atttataacc
     9841 attatatgag caatgaagct tttggattcg tgactttatt tttaacattg caatcgctcg
     9901 tattattgct cgattgtgga gtaactatta ttctctctcg acataccgcg cttaatgttt
     9961 gcaagaaaaa taaaatatcg aataaccaat ggtataacac ggaggtattt cttactattt
    10021 tatatttatt gtttgggttt gtttttttta tcttcaaatg gttaggggtt aactggctgt
    10081 cattaattag ttatacagat cttttgatga ttattgtggc tctttgggct acggttttgc
    10141 aaaatatatg cttaacagtt ttgttggcgg ggaaatattt ctctttatca agtgtgttac
    10201 ttatatctgg taacctttta aaggcaggag gcgctgcttt aatcgttaaa tatttttctc
    10261 caacaattgg ggcatatgta gcaactcaag ctctgcttgg tatatcatta ctttttgtga
    10321 ctcgattttt ctgcgattat tttttgtttt ttaaggatgt gaaaaggaaa attgatttca
    10381 atgtcataaa aaaaataata catgaggggc gagcattagt tatatttaat atttctgccg
    10441 ctgttttatt aaatcttgac aaaataattg tatctcacaa tatatcacta attgcgctgg
    10501 ccccctattt tttagcaagc tcattatgta ttactcctat atctgcattg tcaggtcctg
    10561 taatgcagtt tttccaaccc ggattaacaa aagcaatctt taatgattcg ccaaatgtta
    10621 ttaagacatt acgggtatat atatcgactt tgtgtgtgtc gacactttta ccttcttata
    10681 tcctttgggt gtatcgtgat tcattaattg cattatggct tggaaatact gaattagtta
    10741 atattgtcgc agattatacg aagattttac tgcccggaat agcaattgga ggattaggct
    10801 atatttctta tgttgtatta attgctattg aggattttaa attccaaagt tttctgtctt
    10861 tatctatgac agctatcgtg gttatattag ttgtattttt ctcgatgcga caagatataa
    10921 gaagtatttg ctacgtctat gctttttatc attccctatc tactttatta acatgggcaa
    10981 gatgttatta tctccccaga acccaaaaat atgcgctgga aacggctaag atgttattta
    11041 ttattatgat tgctcttgct gttctttgcg tatttttcaa tctcatcatt aataggattt
    11101 aaagatgttc aaaaataagc ggttaatgat tactggtggt acaggttcct ttggtaacac
    11161 tgttcttaag cgttttctga aaacagatgt taaagaaatc attgttttta gccgcgatga
    11221 gaaaaagcaa gaagatatgc gtattgcttt aggtgattca aaggttaaat tctatatcgg
    11281 cgatactcgc tgctatacaa gtattgagca agcgatgcgt ggcgtagatt atgtgtttca
    11341 tgccgcagcc ttgaagcaag tcccttcatg tgaattttat cctatggagg ctatcaagac
    11401 gaatattctt ggtactgaaa atgtgcttaa tgctgctatt gcaaacaatg tcaaacgcgt
    11461 agttttatta agtactgaca aagcagttta tccaattaat gctatgggta tgtcaaaagc
    11521 tatggctgaa aaggtactga tcgccaaatc acggatgatt gatgctgata aggggccagt
    11581 tctgtgcgca acccggtacg gaaacgtaat ggcttctcgt ggttcagtaa tccctctgtt
    11641 cattgataaa atcaagaata atcaagagct tacaattacc gatcctaaaa tgacacgttt
    11701 tctgatgtct ttagaagatt cggttgactt ggttttacat gcttttgaaa atgggcaaca
    11761 gggggatatc tttgttcaga aggccccggc aagtacaatt gaagacttag ctcaggcaat
    11821 caaaggtttg tttaacagtg aagcttcaaa caaaattatt ggtactaggc atggggaaaa
    11881 gctttatgaa actcttgtat cccgcgaaga aatggtcaaa gcgcaagata tggggaaata
    11941 ctatcgaatc cctgctgata accgcgattt aaattacgat aaatatatcc tggatggtaa
    12001 ggtagaagcc aacaatattg atgattatac atctcataac acaactcgac ttagtgttaa
    12061 agaagttact gagttgttgc taactttaga ttatgtaaaa gagcaattaa attaattata
    12121 ggggctttgc cccttttata tttgaggtta tgttttgaaa ataactaaga cacatttact
    12181 tggctgtata gtgattgagc caaaagtatt tagggatgca cgtggttttt ttgtggaaac
    12241 atatcaaaaa gaaaaatatc aggaaatcgg cattacagaa gattttgttc aagataaccg
    12301 ttcacgttca gcaaaggggg ttttgcgcgg gttacatttt caaacgaatc aaccgcaagg
    12361 caagttagtt tctgttacct caggtgctgt ttatgatgtt gcagttgacc tccggcctga
    12421 atcagctact tttggttcct actttggctt ggttctttct gcagaattaa ataatcaact
    12481 ttatataccg ccaggttttg ctcacggttt ctgtgtattg agcgatagtg cggatttttt
    12541 atataaatgt acacaatatt ataatccagc ttctgaaagt ggtattcaat ggaatgaccc
    12601 aacattaaaa atcaactggc caatttcaaa tccttttata tctgaaaaag attctacact
    12661 gccgtcattc aaagactata tggagaccta cgctcaatga aatacactgt aataggcggt
    12721 tctggattca ttggtagtgc aatgtgtaat tatctaaaaa ataataataa gaatgtcttt
    12781 attccagagc gtgattatca tttttctgca ggtgaagatt taggtattgt tttttattgt
    12841 gcaggctatg gcgattgcat aaataattac gataatgtta ttgacgctaa tgttttgtta
    12901 ttaagaaaaa tacttaataa tgttaaattt aaaaaattag tttatgtgtc ctccacaaga
    12961 gtatacatga atgagaattg tgcaaaagaa aatgctgatc tacttatatg ccataatgat
    13021 aatcgtaaat tatttaatct tacgaagtta gcggctgaag aaatctgcag attaagcaaa
    13081 agaaatgtta ttattgtacg tccatctaat gtgtatggat tagctttgaa tagtaagtta
    13141 tttctccctc aaatcataaa aaatgcaata ttaaataata aaatagatat gtatgttgct
    13201 cctgagtata gcaaggatta tgtttcggtt gatgatttgg tacatattat gttcgaactt
    13261 gctcataaag agtttaatga tgttcaaacg tttaatatag cctctggggt taatacttcg
    13321 gctgcgcaaa ttgccgatat tatcaagcga aagaccaatt gcgaagttat ttggcatgat
    13381 gttaatattc aagagaagtt ccccgtaacg gatataacaa aaattaatga gcttgttgcg
    13441 cacaattata gagatgttat atctgatttg tcgaaaatga tcgatgtgtt ttataatgaa
    13501 ttgcattaaa taattaattt aaaatcatga agaaaatact ttttatttgc cctagttttt
    13561 ttaagtatga attggaaatt tataaagaac tttctttgaa ttatgaagtg gaatattggg
    13621 atgaaagacc taaaaatagc attatattta aggttttact tcggctaaag tttcgaccat
    13681 taattaaaaa agctatcaac cagtattata ttaatctgct gaataacata aatggaaaac
    13741 atttcgatta tgtttttgtt ataaatccgg aagctctaga ttataatact atgaacaagc
    13801 ttaagcagaa gtgtttaggt gttaataagc aatgttattt tgtattatat ctttgggatt
    13861 cactgaaaaa caaacccaat gctcctaaat tgtttccttt gtttgataag gtttcaacat
    13921 ttgatcgcaa agactcggat gactttaata taaatctaat acctttattt tacactcgta
    13981 aatttgatgc aaagtactat ccagtacaac cattaaagta tgatgtttgt tttattggaa
    14041 cagctcactc aaacaggtta aaatatcttg atgaaatttt aaagattata agcaacaata
    14101 aacaaatctc aaaatatatt tttctttttt ttcctagccg catgctattc ttaatcaaga
    14161 agttgttctg taattcaagt actaagcaca aaatttattt tgaatcgctg ccatcagcaa
    14221 aagttgtcga tgctattctt tcatcagcag tcgtaatcga tatacatcat ccctcgcaac
    14281 atggcctaac aatgagaact attgaagccg taggtcttaa caaaaaaata ataacaacta
    14341 atgctgacat taaaaaatac gatttttatg atccgaaaga tgtgtatgtc tatcaatctg
    14401 gtgacagtaa attacctgat gtttttttgt caacgtcaac aactaatttt aagaatcgtg
    14461 agatgtattc actcaaatgt tgggttgaaa ataatttaaa aaaataataa gttactgagg
    14521 tatgaaatgg caaagttaaa aatggatatt cttagatata agcttttatg tggtgatgag
    14581 actaatttaa aataccgtga ttatctaaaa attttctcac caagattatt ttgtgtcttc
    14641 ctgtatcgaa cttcaagttt tttctatcaa aaaagaatga aactactttc taaatttttt
    14701 tcattaatta atttttatta ttcaaagttg aaatttcacc tctatgctcc attggtggag
    14761 ggactttttt taccgcatcc tataagtata gtaatcggtg cgaattcaat aggtgaaaac
    14821 tgtacgattt tccagaacgt aactattgga gcgaaaacgc ttgatattgt atataatcgt
    14881 aatgtaagac cactcatagg taataacgtt actatttcag ccggtgctgt ggttgtgggg
    14941 ccagttgcta taaatgataa tgtttttctt ggtgctaact gcgttgttgt caatagcatt
    15001 gaaagtggtg taatagttgg tggtgtacct gccaaaattt taggtaagct aaccttataa
    15061 aataagtttt ttattacaat aatttttgac ctgaatttta attagtaatt agtattttca
    15121 ttgtttcgct aatctctcat gagaaaatat attcataagt tacttttagt taacattcac
    15181 ctcacaattt tcatcgtgag tgtttgaaat accacccctg acaggagtaa acaatgtcaa
    15241 agcaacagat cggcgtagtc ggtatggcag tgatggggcg caaccttgcg ctcaacatcg
    15301 aaagccgtgg ttataccgtc tctattttca accgttcccg tgaaaagacg gaagaagtga
    15361 ttgccgaaaa tccaggcaag aaactggttc cttactatac ggtgaaagag tttgttgaat
    15421 ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc aggtgctggc acggatgctg
    15481 ctattgattc cctcaagccg tacctcgata aaggtgacat catcattgat ggtggtaata
    15541 cattcttcca ggacaccatt cgccgtaacc gtgagctttc tgccgaaggc tttaacttca
    15601 tcggtaccgg tgtttccggt ggtgaagagg gcgcgctgaa aggtccttcc attatgcctg
    15661 gtggccagaa agaagcctat gaactggttg ctccgatcct gaccaaaatc gccgcagtag
    15721 ctgaagacgg tgagccatgc gttacctata ttggtgccga tggcgcaggt cactatgtga
    15781 agatggttca caacggtatt gaatacggtg atatgcagct gattgctgaa gcctattctc
    15841 tgcttaaagg tggcctgaat ctctctaacg aagaactggc acagaccttt accgagtgga
    15901 ataacggtga actgagcagc tacctgatcg acatcaccaa agacatcttc actaaaaaag
    15961 atgaagacgg taactacctg gttgatgtga tcctggatga agcggctaac aaaggtaccg
    16021 gtaaatggac cagccagagc gcgctggatc tcggcgaacc gctgtcgctg attaccgagt
    16081 ctgtgtttgc acgttatatc tcttctctga aagagcagcg tgttgccgca tctaaagttc
    16141 tctctggccc gcaagcgcag ccagctggcg acaaaggtga gttcatcgag aaagttcgtc
    16201 gtgcgctgta tctgggtaaa atcgtttctt acgctcaggg tttctctcag ctgcgcgcag
    16261 cgtctgaaga gtacaactgg gatctgaact acggcgcaat cgcgaagatt ttccgtgctg
    16321 gttgcatcat tcgtgcgcag ttcttgcaga aaatcaccga tgcttatgcc gaaaatccgc
    16381 acatcgctaa cctgctgctg gctccgtact tcaagcaaat tgccgatgac taccagcagg
    16441 cgctgcgtga tgtcgttgct tatgcagtac agaacggtat cccggttccg accttcgctg
    16501 ctgcggttgc ctattacgat agctaccgtg ccgctgttct gcctgcgaac ctgatccagg
    16561 cccagcgcga ctatggcgat tgtggctgca gggatca
//
