LOCUS       HQ388393               14179 bp    DNA     linear   BCT 05-APR-2011
DEFINITION  Escherichia coli strain G1207 serogroup O11 O-antigen gene cluster,
            complete sequence.
ACCESSION   HQ388393
VERSION     HQ388393.1  GI:317408215
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14179)
  AUTHORS   Li,Y., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Liu,B., Wang,L. and Knirel,Y.A.
  TITLE     Structural and genetic relationships of two pairs of closely
            related O-antigens of Escherichia coli and Salmonella enterica : E.
            coli O11/S. enterica O16 and E. coli O21/S. enterica O38
  JOURNAL   FEMS Immunol. Med. Microbiol. 61 (3), 258-268 (2011)
   PUBMED   21205000
REFERENCE   2  (bases 1 to 14179)
  AUTHORS   Li,Y., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Liu,B., Wang,L. and Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin,
            Tianjin 300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..14179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1207"
                     /db_xref="taxon:562"
                     /note="serogroup: O11"
     gene            1138..2373
                     /gene="wzx"
     CDS             1138..2373
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADV17648.1"
                     /db_xref="GI:317408216"
                     /translation="MLKNIIYLLSVQGGNYIFPLVTLPYLVRILEPTGYGIYGYSFAV
                     VQYFILFIDYGFNYSAPKIISISRENTDSISKVFWNVTFIKIIAASLGLLIIYLISEL
                     NIIDYQIKFIILAYISVVGNLIYPVWFFQGLEKMLGIAISMFSARLLTLCLTFLLVKS
                     EKDLGIAITIQSSITVIAGIISTYFLFSLKKIIWVPPSLSKMKELIYDGWHYFISSAA
                     ISLYTTSSTIILGALSGTTAVGYFIAADKIRLALQGVIGPITQAVFPRVTYIMKDNHD
                     DGLLLARKVFRWQFSLMLVLSIFLYFFADDIILIMYGKTYHESLEILKVLAFTPAIVT
                     VSNYIAILVMIPIGMKKEFTKIITTSGLIGLLIITYLTNEYSGVGTAIGVISTEFIIM
                     ARFYFLIKKTNKLNNLNLL"
     misc_feature    1138..13701
                     /note="O-antigen gene cluster"
     gene            2382..3107
                     /gene="wdaD"
     CDS             2382..3107
                     /gene="wdaD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaD"
                     /protein_id="ADV17649.1"
                     /db_xref="GI:317408217"
                     /translation="MNKPTLTIVTVVYNGEMYIEDTIKSVLSQNCNDDIEYIIIDGAS
                     TDNTLNIVNSYKNEISYIISENDNGIYDAMNKGVLKASGNYIGFINADDFYNKDVLCQ
                     VIKVMKENKYDVIYGNMSLVEADSLDVIQVRRPKDWKLHIDMNLSHPATFIKKDIHRE
                     NLFNTSYKIASDYDLLLKLKKQGYIFHYIDKNISSMRNAGLSAKNVKLALEEARQIRK
                     KRNGKLYAALADIYLFMRKVIKR"
     gene            3104..4339
                     /gene="wzy"
     CDS             3104..4339
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADV17650.1"
                     /db_xref="GI:317408218"
                     /translation="MINRNIKISASFISIIYLPLALLLAMIEMINGARWSVYVCSFML
                     GVLAFLLIPYENWDLTRHYETYLYVSQTSLNKVFENSDNRYITINTVIYLFKSLGIKK
                     EFLPFFSTFLSYIFYFNIYVNYIKKYNPELSHRCIYLYILISVIPFFAIASSLRQYLA
                     FSIMLYMIVQYFTSENNRYKSQKIIFSAIISCCIHPSAILLLGVFFVSKLIRLNKTIL
                     YLIILVLILNHDGLISSLLFQLIRPLLESLGFYFPEYMDSVTIGISNSLLSTNEYILN
                     KIILPSVFFIFTFIYLILFNKKMTRQESQIKNFCSLLLLLICLLSLSPDLFYRFSIFW
                     VLLYSYIFFSYDINKISKLCKHLIFLILIAACSIINLAQANMGKDIIYVSWCDILYKP
                     SLFLFNKEVKSAEYIRVSQ"
     gene            4341..5408
                     /gene="wdaE"
     CDS             4341..5408
                     /gene="wdaE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaE"
                     /protein_id="ADV17651.1"
                     /db_xref="GI:317408219"
                     /translation="MSIGVFKLYKNKIGGTEKAVENLIDILTKNSLQVRCFSAINAQE
                     IQVREDNRKNYLFQDNTAYEESKLRFYIKYLFFLSAKSKDLELIISTDIATSSLLLLI
                     GFFRYNKNKIICWEHYPFDKNSIFWRKVFKFLLSLNKKAVVVCNSHKEKDKFSFCKRV
                     VIIPNSIKKQKYKRIHQGKETLDLIYIGRIVKDKGVQRLINAVINHPSTKKYVLHIVG
                     GGQELEYLKSKYKNNNQIHFHGPSLNTDVFLAKSDVFVSGSYFECFPVTILEAMSYGL
                     PVISMDISGGTSSVISSSGGGFVCQSQIEFQEKLTYFEDNVKRTEHGNAGFLFVNRHY
                     SEEIVSAQWMSLINRANSQEN"
     gene            5429..6445
                     /gene="gne"
     CDS             5429..6445
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADV17652.1"
                     /db_xref="GI:317408220"
                     /translation="MSILVTGGAGYIGSHAILTLLQNGYDVISLDNYCNSSPKSLERV
                     EEICLSKIKKLRGDIRDRHILKNIFSKYNVSTVIHFAGLKSVNESIKKPLEYYDTNLL
                     GTLILLDEMQKANISNLIFSSSATVYGNPHYVPISEKHPVGEVINPYGRSKFMVEHIL
                     RDFCNANSDANITILRYFNPVGAHETGLIGEDPLGTPNNLFPYVAQVAIGKLPYLNVF
                     GNKYMTKDGTGVRDYIHVVDLVEGHLAALKNLNRNRGLKVFNLGTGQGYSVLDIIDAF
                     KLETGRDIPFRIVSERNGDVAECWSDPSLANEQLSWVAKRTLNNMVEDAWRWQTMNPN
                     GYKE"
     gene            6514..7521
                     /gene="wdaF"
     CDS             6514..7521
                     /gene="wdaF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaF"
                     /protein_id="ADV17653.1"
                     /db_xref="GI:317408221"
                     /translation="MKILYDGIVKSLQSYGGITVYFEEIIKRLKNDEYKFIQFNDKNE
                     NHNDDLHRPCRFLERYRCFEALKSDSDYSIFHSTYYRVPKDGEYAVVTTVHDFTYELY
                     MKGLRRNVHSLQKRWAVKNSDAIICVSNNTAKDLINFFKVDEKKIHVIHNGVSEDYRH
                     LNLEKENFVLFVGARSGYKNFELAVKAVSKLFDTNLKIVGGGGLNKAEMKLLSEYLPE
                     RFQYLGKVSNEKLNILYNSAYCLIYPSEYEGFGIPLLEAMRTGCPVIAVNRSSIPEVV
                     GDSAILVEEVSIESLFSALLSVEANANELKNKGLAQSNKLSWGKCFSETYNLYKSLSK
                     F"
     gene            7532..8653
                     /gene="gmd"
     CDS             7532..8653
                     /gene="gmd"
                     /note="GDP-mannose-4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ADV17654.1"
                     /db_xref="GI:317408222"
                     /translation="MCKKALITGITGQDGSYLAEFLLKKGYEVHGIKRRSSLFNTDRI
                     DHIYQDPHHRNLKLQLHYGDLTDTSNLTRILSEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYL
                     GNMDSLRDWGHAKDYVRMQWMMLQQDQPEDFVIATGKQISVREFVRMSAKEVGLELEF
                     SGTGVDEIAFVVNKTSDCAVGVNIGDVIVRVDPRYFRPAEVETLLGDPTKAKNVLGWE
                     PEITVEEMCAEMVASDLEKAKQHALLKSHGFDVAVSLER"
     gene            8657..9622
                     /gene="fcl"
     CDS             8657..9622
                     /gene="fcl"
                     /note="GDP-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ADV17655.1"
                     /db_xref="GI:317408223"
                     /translation="MTKKRIFVAGHRGMVGSAICRQLLARNDVELIVKTHKELDLTVQ
                     KDVECFFEQERIDQVYLAAAKVGGIHANNTFPAEFIYQNLLIESNIIHSSYKAGIKKL
                     LFLGSSCIYPKFAEQPMKESALLTGTLEPTNEPYAISKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGMNDNFHPNNSHVIPALMRRFHEAKELGLNEVVVWGTGTPKREFLYVDDMA
                     AASVYVMELDDEIYKKNTQPMLSHINVGTGVDCSIREMAETMALVVGYDGKIVFDITK
                     PDGSPRKLMDVTRLENLGWKYRYNLKQGLELTYKWFINNLDSFRS"
     gene            9624..10079
                     /gene="gmm"
     CDS             9624..10079
                     /gene="gmm"
                     /note="GDP-mannose mannosylhydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="ADV17656.1"
                     /db_xref="GI:317408224"
                     /translation="MNKRLETELFKSIVEHTPLISIDLIIRNEDGKALLGQRLNRPAQ
                     NYWFVPGGRILKDESFENAFKRVTFEELGVQISINEAKFLGIYEHFYSDNFSGTCFST
                     HYVVHGYEISLMPHQINYPTLQHSTYNWFDIAELLANSSVHQYTKNYFK"
     gene            10091..11476
                     /gene="manC"
     CDS             10091..11476
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADV17657.1"
                     /db_xref="GI:317408225"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLTSRLTMLQETLRRL
                     EGVEHRPALVICNEVHRFIVAEQMRNVNLANSGILLEPIGRNTAPAVALAALKAISSG
                     EDPILLVLAADHEIQDQKRFISSILAAKEFAEEGKLVTFGIVPTKPETGYGYIKTGEN
                     LNEYGFKVSAFVEKPELDVAKKYLEDGDYLWNSGIFMFRASVFIDELNKFRPDILKIC
                     QQALKSSTQDLDFIRIDNDSFSCCPEESIDYAVMEKTTEAVVVPLNAHWSDVGSWSAL
                     WEISRKDKSGNAIRGDVLIHDSSDSYLYSQHRLIGVVGVKDLVVVETKDAILVAHKDK
                     VQQVKNIVEKLKVNNRTEYLQHREIFRPWGSHDSIAEGSRFEVKHVVIHPGHKTAKQI
                     HYHRTEHWIVVSGTAKVHYEDEIFLVSENESTYIPIGVPHFIENPGKIPLEIIEVRSG
                     VYLDEDDVVRI"
     gene            11560..12306
                     /gene="wdaG"
     CDS             11560..12306
                     /gene="wdaG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaG"
                     /protein_id="ADV17658.1"
                     /db_xref="GI:317408226"
                     /translation="MKVSIITVTYNSEKTLRDTLESIELQTYKDIEYIIIDGGSTDNT
                     LKLINEVSTRVTKCLSESDNGIYDALNKGINLSTGDIIGFVHSDDLLARPDIIETIVS
                     RFHETKADVVYGDLVFFEKNQIDIIKRYWRSGPFKRSKLSLGWAPPHPSFYMRRELYK
                     DDGYFDLSYRIAADYDQMVRILKRDDIKVIYVPQVFVKMRLGGESTRIDNAISSTKEI
                     VEVMKNHNVNWKIAIIIRKISKLMQLFAHK"
     gene            12331..13701
                     /gene="manB"
     CDS             12331..13701
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADV17659.1"
                     /db_xref="GI:317408227"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVEFIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFTRGALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESHGDVPLMEEKTKLI
                     LELLNK"
BASE COUNT     4556 a   2306 c   2874 g   4443 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggtgaac ctttaggttt gggccactcc attttgtgtg cacgccccgt cattggcgac
      241 aacccatttg tcgtggtgct gccggacgtt gtgatcgacg acgccagcgc cgacccgttg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccgctg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcaacc tggtgcatgg gggcgtattc agctgactga tgctattgct
      601 gagctggcaa aaaaacaatc cgttgatgca atgctgatga ctggcgacag ttacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactacgcaa cttgaaagaa
      721 ggggcgaagt tccgcaaagg tattgagaag ctgttaatcg aataatgaaa atctgaccga
      781 atgtaacggg tgataagaaa attataacgg cggtgaagat tggtggggct aattgtactc
      841 attgcctcat tctttcttta taccgttcta aatatgagta acaataactt ttcaataggc
      901 atttattgtg ggaggctgag attttctcca tctctgaagc gatttggtaa aacgttcaga
      961 gttaagcttt tcgaactaac cataacggtt aatgctagtt acaattagga catgcatgca
     1021 gtacgctggt agctgttgag ccaggggcgg tagcgtgaat taaatgttta tcccatatta
     1081 cttaacaaat tgtaaactat cgtgcaaaat aagacttgtt aagtacgtag gctcgttatg
     1141 ttaaaaaaca tcatctattt attaagcgtt caaggtggca attatatatt tccattggtc
     1201 acactgcctt atcttgtcag gatccttgaa cctacaggat acgggatata tgggtatagt
     1261 tttgctgtgg tccaatattt tatattattt attgattacg gatttaacta ctcagcgccc
     1321 aaaatcatat caatatcaag ggaaaatact gattccataa gtaaagtttt ttggaatgta
     1381 acgtttatca aaataattgc agcatctctt ggtttgttaa ttatttattt aatttcggaa
     1441 ttgaatatca ttgattacca aattaaattt atcattcttg catacatatc tgtcgtagga
     1501 aatttaatat atccagtatg gttttttcaa gggctggaaa aaatgttagg aatagcgata
     1561 agtatgtttt ccgcacgctt acttacacta tgtttaacat ttttattggt taaaagtgaa
     1621 aaggatcttg gtatagcgat aacaatacaa tcatcaataa cagttattgc aggtataata
     1681 tctacatatt ttttattttc tcttaaaaaa ataatatggg taccaccatc cttatccaaa
     1741 atgaaagagt taatttatga tggatggcat tacttcattt cctctgcagc aataagcctt
     1801 tatactacta gttcaacaat aatattaggt gcattatcag gaacaactgc agtgggatat
     1861 tttatcgctg cagataaaat taggcttgca ctacaaggcg ttattggtcc tataacccaa
     1921 gctgtttttc ctagagtaac ttatataatg aaggataatc atgacgatgg actattatta
     1981 gccaggaaag tgtttcgatg gcaattttca ctgatgttag tactttctat atttctttac
     2041 ttttttgcag acgatataat tttaatcatg tatggaaaaa cgtatcatga atctttagaa
     2101 atactcaagg ttttagcttt tactccagca atagttacgg taagtaatta tattgcaatt
     2161 ttagtaatga tccctattgg tatgaaaaaa gagtttacaa aaattattac taccagtgga
     2221 ttaataggcc tactgataat tacttatttg actaatgaat actcaggagt tggtacagca
     2281 ataggagtta tttcaactga gtttataata atggcgagat tttacttttt gattaagaaa
     2341 acaaataaat taaataattt aaatttattg tagggctaac aatgaataaa ccaactttaa
     2401 caatagttac cgttgtgtac aatggcgaaa tgtatatcga agatactata aaaagtgtct
     2461 tatcacaaaa ctgcaatgat gacattgaat atattataat tgatggtgca tcaacggata
     2521 atactctaaa tattgttaat tcttacaaaa atgaaatttc atatatcatt tctgaaaatg
     2581 ataatggtat atatgatgca atgaataaag gtgtgttaaa agcatctgga aattatatag
     2641 gatttataaa cgctgatgat ttttataata aagatgtatt atgtcaagta ataaaagtaa
     2701 tgaaagaaaa taaatatgat gttatttatg gaaatatgtc tttggtagag gctgatagct
     2761 tggatgttat tcaggtccgg aggccgaaag actggaaact acatatagat atgaatttaa
     2821 gccatccggc cacatttatt aaaaaagata tccacagaga aaatttattt aatactagct
     2881 ataaaattgc ttctgattat gatcttttgc taaaattgaa gaaacaaggt tatatctttc
     2941 attatattga taaaaatatc tcttcgatga gaaatgccgg ccttagcgct aaaaatgtaa
     3001 aattagcatt ggaggaagcg agacaaatcc gaaagaaaag aaacgggaaa ttatatgcgg
     3061 cgttagcaga tatttaccta tttatgagaa aagttataaa aagatgatta atagaaacat
     3121 aaaaattagt gcttcattta tttcaataat ttatttgcct cttgctttat tattagcaat
     3181 gattgaaatg attaatgggg ccagatggag tgtttatgta tgttcattta tgctaggggt
     3241 actagcattt ttgttaatac cttatgaaaa ttgggatttg actaggcatt atgaaacata
     3301 tttatatgtt agtcaaacat cactaaacaa agtttttgaa aatagtgaca atagatatat
     3361 tacaataaat actgttattt atttatttaa atctttagga ataaaaaaag aatttttacc
     3421 ttttttttca acatttttat catatatttt ttattttaat atatatgtta attacattaa
     3481 aaaatacaat ccggaattat ctcatagatg tatatattta tatattctaa taagcgtaat
     3541 cccatttttt gctatagcta gttcattgcg gcaatatctt gcatttagta ttatgcttta
     3601 tatgattgta cagtatttca ccagtgaaaa taataggtat aagtcacaaa aaattatatt
     3661 ttctgcaatt atttcatgtt gtattcatcc cagcgcaata ctattgctag gtgttttttt
     3721 tgtcagcaaa ttaataagac ttaacaaaac aatattgtat ttaataatat tagttctaat
     3781 ccttaaccat gatggattaa tatcttcttt gctatttcaa ttgattaggc cattattgga
     3841 aagcttaggt ttttactttc ccgaatatat ggactctgta accataggca taagcaatag
     3901 cttgctgagc actaatgaat atatcttgaa taagatcata ttaccgtcag tattttttat
     3961 ttttactttt atctatttaa ttttatttaa taaaaaaatg acgaggcaag agtcacaaat
     4021 caaaaatttt tgcagtttac ttcttttgct gatatgcctt ttatcgttat cgcctgattt
     4081 attttatcga ttttccattt tctgggtgtt attatatagt tatatatttt tctcctacga
     4141 tattaataaa attagcaaac tatgtaagca tctgatcttc ttaatactca ttgccgcatg
     4201 ttcaatcata aatttggcac aggcaaatat gggtaaagat attatttatg tgtcttggtg
     4261 cgatatattg tataagccct cattattcct attcaataaa gaagttaaat cagcagaata
     4321 tataagagtg agtcaataaa atgtccattg gagtctttaa gctgtataaa aataaaatag
     4381 gtggcactga gaaagcagtt gaaaatctaa ttgatatatt aactaaaaac agcctacaag
     4441 tgagatgttt ttcagcaata aacgcacaag agatacaagt tagagaagat aatcggaaaa
     4501 actacttatt ccaagacaat actgcatatg aagaaagcaa attaaggttt tacattaaat
     4561 atttgttttt tttgagtgcc aaaagtaaag accttgaatt aataatttcc acggatatag
     4621 ctacatcatc attattgtta ttgattggtt tttttcggta taataagaat aaaattattt
     4681 gctgggaaca ttatccattt gacaagaata gtattttttg gagaaaagta tttaaatttc
     4741 ttttgtctct taataaaaaa gctgtagtag tttgtaattc tcacaaggaa aaagataagt
     4801 ttagtttttg taaaagggtt gtaattatac caaattcaat caaaaagcaa aaatataagc
     4861 gcatacatca aggaaaagaa actcttgatt tgatttatat tggtcggatt gttaaagata
     4921 aaggagttca aaggttaatt aatgcagtaa taaatcaccc gtctacaaaa aagtatgttt
     4981 tacacattgt aggaggtggt caagagctgg agtatctcaa atctaaatat aagaataaca
     5041 accaaataca ctttcatggg ccgagtttaa acacagatgt ttttttggca aaaagtgatg
     5101 tttttgtatc cggttcatac tttgaatgtt ttcctgttac aatacttgaa gctatgtcct
     5161 acggattacc tgtaatttca atggatatat ctggagggac aagtagcgtg atttcttcat
     5221 cgggtggcgg atttgtgtgt caatctcaaa ttgaatttca ggaaaaacta acttactttg
     5281 aagataatgt taaacgaacc gaacacggaa atgcaggatt cctattcgtg aatagacatt
     5341 attcagaaga aatagttagt gctcagtgga tgagtttgat caatcgagcc aatagtcaag
     5401 aaaattaagg tattggagct attaaagtat gagcatactt gtgactggtg gggctggcta
     5461 tattggctca catgctatat taactttatt gcaaaatgga tatgatgtta tatcgctaga
     5521 taattattgc aattcctctc caaaatctct agaacgagta gaggaaattt gcttatctaa
     5581 gataaaaaaa ttacgtggtg atattagaga tagacatata ttaaaaaata tcttttcaaa
     5641 atataatgtt tctactgtaa tacattttgc gggtttaaaa tctgttaacg agtccattaa
     5701 aaaaccatta gaatattacg acacaaatct tttggggact ctaatattac ttgatgaaat
     5761 gcagaaagcg aatatttcta atctaatttt tagttcgtct gctactgtct atggcaatcc
     5821 tcattatgtt ccaatatctg aaaaacaccc tgtcggtgag gtgatcaatc cgtacggtcg
     5881 ttcaaagttt atggtggaac atatattgcg tgatttttgt aacgcgaata gcgacgcaaa
     5941 tatcacaata ctaagatatt ttaatcctgt aggtgctcat gagactggat taattggaga
     6001 agatccttta ggaactccta ataacctgtt tccctatgtt gcgcaagtcg caattggaaa
     6061 attaccctat ctaaatgttt ttggtaataa atatatgaca aaagatggaa caggtgtaag
     6121 agattatatc catgtagtcg atttagttga ggggcatctt gccgctttaa aaaatctcaa
     6181 tagaaaccgg gggcttaaag tttttaattt aggcactggt caaggttata gtgtgttaga
     6241 tatcatcgac gcttttaaac tggaaacagg acgcgatata cctttccgta tcgtttctga
     6301 gcgaaatgga gatgtggctg aatgttggtc cgatccttcc ttagcaaacg agcagctaag
     6361 ttgggttgct aaaaggactc ttaataatat ggtggaagat gcatggagat ggcaaacaat
     6421 gaatccaaat ggatataaag agtaaattga aacaattata atcatccatt tcattgctta
     6481 ctgtattgtt atctgtatat atgaaaacaa aaaatgaaaa tattatatga tgggattgtg
     6541 aaaagtttac aatcatatgg tggtatcact gtatactttg aagaaatcat taagagatta
     6601 aaaaatgatg aatacaaatt cattcaattt aatgataaaa atgaaaatca caatgatgat
     6661 ctccatcggc catgccggtt tttagaacga tatagatgtt ttgaagcatt aaaaagtgat
     6721 tcagattaca gtatatttca ttctacatat tatcgggttc cgaaagatgg ggaatatgcg
     6781 gttgtgacca cggtccacga tttcacatat gaattgtata tgaaaggact tcggcgcaat
     6841 gttcatagct tacaaaaaag atgggctgtg aaaaatagcg atgcaatcat ttgtgtctct
     6901 aataatacag ccaaggattt aattaatttc tttaaggttg atgaaaaaaa gatacatgtt
     6961 attcataatg gtgtatcgga agattataga cacttaaatc tagaaaaaga aaattttgtg
     7021 ctttttgtcg gtgctcgttc aggatataaa aattttgagc ttgcagttaa agcagtttct
     7081 aaactttttg atactaatct taaaatagtc ggcggaggtg gtttaaataa agcagaaatg
     7141 aagttactat ctgaatatct tccagaaaga ttccagtact tagggaaggt atcaaatgaa
     7201 aaactaaata tattatataa ttcggcttat tgcttaatct atccttcaga atatgaaggg
     7261 tttggaattc ccttgcttga ggctatgcga actggctgtc ctgtgatagc cgtaaataga
     7321 tcatcaatac ccgaggttgt gggggatagt gcgattttag tcgaagaagt atcaattgaa
     7381 agtttattct cagctttatt aagtgtagaa gctaacgcta atgaattaaa aaataaagga
     7441 ttagcgcaat ctaataaact ttcatggggg aagtgtttct ccgaaacata taatctatat
     7501 aaatcactat ctaagttttg agaggttatt tatgtgtaag aaagctttaa ttacaggaat
     7561 taccgggcaa gatggttcct acttggcaga atttctgctt aaaaaaggct atgaggttca
     7621 tggaataaaa cgtcgatctt ccttgtttaa tactgaccgt attgatcata tatatcaaga
     7681 cccacaccat cgtaatttaa aattacaact tcattatggt gatttaacag atacttctaa
     7741 tttgactcgg atattatctg aagttcaacc tgatgaagtt tataatcttg gcgcaatgag
     7801 tcacgtagct gtttccttcg aatccccaga atatacagct gatgttgatg ctataggtac
     7861 tttacgatta ttggaagcta ttcgtttttt gggattagaa aaaaaaaccc gattttatca
     7921 ggcgtcaacc tctgaattgt atggattagt tcaggaagtt ccacaaaaag agacaacgcc
     7981 gttctatcct cgttcacctt atgccgtagc taaactgtat gcatattgga tcactgtaaa
     8041 ctatcgtgaa tcttatggaa tgtatgcatg taacggtatt ttgttcaatc atgaatcccc
     8101 acgtcgtggg gaaacgtttg taacacgtaa aatcacacgt gctatagcta atatttcgca
     8161 ggggatagaa aaatgcttat atcttggcaa catggattct ttacgtgatt gggggcatgc
     8221 gaaagattat gttcgtatgc aatggatgat gcttcaacaa gaccagccag aagactttgt
     8281 tattgccacc ggcaaacaaa tatctgtacg tgaatttgtc cgtatgtctg ctaaagaagt
     8341 tggacttgaa cttgagtttt ccggtactgg tgttgatgag attgcatttg ttgtaaataa
     8401 aacatcagat tgcgccgttg gtgttaatat tggtgatgtt attgttcgcg ttgaccctcg
     8461 ttattttcga ccagcagaag ttgaaactct tcttggtgat ccaacaaaag caaaaaatgt
     8521 cttgggttgg gagccagaga ttacagttga agaaatgtgt gcagaaatgg tggctagtga
     8581 tttagaaaaa gctaaacagc atgcacttct taaaagccat ggttttgacg tggcagtctc
     8641 tttggagcgt taaagtatga ccaagaagcg aatttttgtg gctggacatc gtgggatggt
     8701 tggttctgct atttgtcgtc aattattagc acgtaatgat gttgagttaa ttgtcaaaac
     8761 tcacaaagaa ctcgatttga cagttcaaaa ggatgttgaa tgtttttttg agcaagagag
     8821 aatagatcag gtttaccttg cagcagcaaa agtaggagga attcatgcta ataacacttt
     8881 tccagctgaa tttatttatc agaaccttct tattgaaagc aatattattc actcatctta
     8941 caaagcgggc ataaagaaac tattattctt agggtcaagt tgtatctatc caaagtttgc
     9001 agaacagcca atgaaagaat ctgcactttt gacgggaact cttgagccta ctaatgagcc
     9061 ttatgctatt tccaaaatag caggtataaa gctatgtgaa tcttataatc gccaatatgg
     9121 atgtgattat cgcagtgtaa tgcctactaa cttatatggt atgaatgata attttcatcc
     9181 caataactca catgtaattc cagcgcttat gcgacgtttt catgaagcaa aggaacttgg
     9241 tttaaatgaa gtggttgtct ggggaacggg aactccaaaa cgcgagtttt tatatgttga
     9301 tgatatggca gctgcatcag tatatgtgat ggagcttgat gatgaaattt ataaaaaaaa
     9361 tactcaacca atgttatctc atattaacgt aggtactggt gttgattgtt ccatacgtga
     9421 aatggcagaa acaatggctt tagttgttgg ctatgatgga aaaattgttt ttgatataac
     9481 aaaaccagat ggttcgcctc gtaaacttat ggatgttaca cgccttgaga atttgggttg
     9541 gaaatatcgt tataatttaa aacaaggact agaattaaca tataaatggt ttataaataa
     9601 tcttgactca tttaggagtt aatatgaaca agagattaga aacagagtta tttaaatcaa
     9661 tagttgaaca tactccacta atctcgatag accttattat aagaaacgaa gatggtaagg
     9721 cgctgcttgg tcagcgcctt aatcgtccag cacaaaatta ttggtttgtg ccggggggac
     9781 ggattcttaa agatgagtct ttcgagaacg cattcaaaag agttactttt gaagaattgg
     9841 gtgttcaaat tagcattaat gaagcaaaat ttcttgggat ttatgaacat ttctacagcg
     9901 acaatttttc tgggacttgt ttttcaactc attatgtcgt acacggatat gagattagtt
     9961 tgatgccaca tcaaattaac tatccaacac tacaacatag tacttacaat tggttcgata
    10021 tagccgaatt gttagctaat tcctcagttc atcagtacac aaaaaattat tttaagtgaa
    10081 ggttatttat atgctacttc ctgttgtaat ggccggtggt tctggaacta gattgtggcc
    10141 actttctcga acactatatc ctaagcaatt cctatcgtta actagtcgtt tgactatgct
    10201 acaagagacc ttaagaaggc ttgaaggagt tgaacatcgt cctgctctgg ttatttgtaa
    10261 tgaagttcat cgttttatcg ttgcagagca gatgcgtaat gtaaatttag cgaatagtgg
    10321 cattttacta gagccgatag gtcgtaatac agcccccgca gttgcgcttg ctgctcttaa
    10381 agcaatttca tcaggtgagg atccaatact gcttgtacta gcagccgatc atgaaataca
    10441 agaccaaaag cgctttatat cgtcaatatt ggctgcaaaa gaatttgcag aggaaggtaa
    10501 gctggtaact tttggtattg tgccaaccaa acccgaaact ggatatggct acataaaaac
    10561 cggagaaaat ttaaatgaat atggttttaa agtttctgct tttgttgaaa agcctgagct
    10621 agatgtggcc aaaaaatatc ttgaagatgg agattatctt tggaatagtg gaatattcat
    10681 gtttagggct tctgtgttta ttgatgagtt gaataaattt agaccagaca tactaaaaat
    10741 atgccaacaa gcattgaagt cctctacaca agatcttgat tttatccgta tagataatga
    10801 ctcatttagt tgttgccctg aagaatccat tgattacgct gttatggaga aaacaacaga
    10861 ggctgtggta gttccattaa atgcccactg gagcgatgtt ggttcttggt ctgcattatg
    10921 ggaaattagc agaaaagata aaagtggaaa tgcaattaga ggagatgtat taattcatga
    10981 ttcttctgac agttatcttt actcacaaca tagattaatt ggtgtagtag gagttaaaga
    11041 tttagtagtt gttgaaacta aagatgctat tctcgttgcg cataaagata aagttcaaca
    11101 ggtaaaaaat attgtagaaa aacttaaagt taataaccgt acggaatatc tgcagcatcg
    11161 tgaaatattt aggccatggg gcagtcatga ctctatagcg gaaggatctc gatttgaggt
    11221 taaacatgtc gttattcatc ctggacataa aactgctaaa cagattcatt accatcgtac
    11281 tgagcattgg attgttgttt ctggaacagc aaaagtccat tatgaagatg aaatatttct
    11341 tgtttctgaa aatgaatcaa cttatatacc aataggtgta cctcatttta ttgagaatcc
    11401 aggaaagatc cctttggaga ttattgaggt acgttcaggt gtctacttgg atgaagatga
    11461 tgttgttaga atctaaaatg atgctggata ctaatgcaac attattattc gtaatgctgt
    11521 actgacgtta tttaattatg attattattg gatgttaaaa tgaaagtcag tattattaca
    11581 gtgacttata atagtgaaaa gactttaagg gatactttag agtcaataga gttgcaaacc
    11641 tataaagata tagagtatat aattattgat ggtggttcta cagataatac gttaaaacta
    11701 attaacgaag tatcaacaag agtaaccaaa tgtctatcag aaagcgataa cggtatctat
    11761 gatgctttaa ataaaggtat aaatttatct acgggagata ttattggatt cgttcattct
    11821 gatgaccttt tagcaagacc tgatattatt gaaactattg taagccgttt ccatgagaca
    11881 aaagcggatg ttgtatacgg tgatttggtt tttttcgaaa aaaaccaaat tgatataatc
    11941 aaacgatact ggcgtagtgg tccttttaaa cgttctaagc tatcattagg ctgggcacca
    12001 ccgcatccat ctttctatat gaggagggaa ttatataaag acgatggata ttttgattta
    12061 tcctatagga ttgctgcaga ctatgatcag atggtcagga ttttgaaacg tgatgatatt
    12121 aaagttattt atgttcctca agtatttgta aagatgagat tgggtgggga aagtaccaga
    12181 attgataacg cgatatcaag tacaaaggaa attgtagaag ttatgaaaaa ccataatgta
    12241 aattggaaaa ttgctatcat tatcagaaaa atctcaaaac taatgcaatt gtttgcgcat
    12301 aaataatttt attatcggta aggtttttat atgaaaaaat taacctgctt taaagcctat
    12361 gatattcgcg ggaaattagg cgaagaactg aatgaagata ttgcgtggcg cattggtcgc
    12421 gcctatggcg aatttctcaa accgaaaacc attgtgttag gcggtgatgt ccgcctcacc
    12481 agcgaaacct taaaactggc gctggcgaaa ggtttacagg atgcgggcgt cgatgtgctg
    12541 gatattggca tgtccggcac cgaagagatc tatttcgcca cgttccatct cggcgtggat
    12601 ggcggcatcg aagttaccgc cagccataac ccgatggatt acaacggcat gaaactcgtg
    12661 cgcgaagggg ctcgcccgat cagcggtgat accggactgc gcgatgtcca gcgcctggca
    12721 gaagccaacg actttcctcc cgtcgatgaa accaaacgcg gtcgctatca gcaaatcaat
    12781 ctgcgtgacg cttacgttga tcacctgttc ggttatatca acgtcaaaaa cctcacgccg
    12841 ctcaagctgg tgattaactc cgggaacggc gcagcgggtc cggtggtgga cgccattgaa
    12901 gcccgattta aagccctcgg cgcaccagtg gaattcatca aagtgcacaa tacgccggac
    12961 ggcaatttcc ccaacggtat tcctaacccg ctgctgccgg aatgccgtga cgacacccgt
    13021 aatgcggtca tcaaacacgg cgcggatatg ggcattgcct ttgatggcga ttttgaccgc
    13081 tgtttcctgt ttgacgaaaa agggcagttt atcgagggct actacattgt cggcctgctg
    13141 gcagaagcat tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc gcgtctctcc
    13201 tggaataccg ttgatgtggt gactgctgct ggcggcactc cggtgatgtc gaaaaccgga
    13261 cacgccttta ttaaagaacg tatgcgcaag gaagacgcca tctacggtgg cgaaatgagc
    13321 gcccaccatt acttccgtga tttcgcttac tgcgacagcg gcatgatccc gtggctgctg
    13381 gtagccgaac tggtgtgtct gaaaggaaaa acgctgggcg aactggtgcg cgaccggatg
    13441 gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg cgcaccccgt tgaggcgatt
    13501 aaccgcgtcg aacagcactt tacccgtggg gcgctggcgg tggatcgcac cgatggcatc
    13561 agcatgacct ttgccgattg gcgctttaac ctgcgcacct ccaacaccga accggtggtg
    13621 cggttgaatg tggaatctca cggtgatgtg ccgctgatgg aagaaaagac aaaacttatc
    13681 cttgagttac tgaacaagta attcagtaat ttcatataaa taagttttaa atgacggaaa
    13741 aggtgagaca ttcagtgtgg tatagcaaag gtaatgctat tcaccatctc tatgagtgag
    13801 ttaacatcta taccacattt aagccgcaca ctcggcggtg accaccccct gacaggagta
    13861 aacaatgtca aagcaacaga tcggcgtagt cggtatggca gtgatgggac gcaaccttgc
    13921 gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgaaaagac
    13981 ggaagaagtg attgccgaaa atccaggcaa gaagctggtt ccttactata cggtgaaaga
    14041 gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg
    14101 cacggatgct gctattgatt ccctcaaacc atatctcgat aaaggtgaca tcatcattga
    14161 tggtggtaac accttcttc
//
