LOCUS       AB812049               16588 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H711c.
ACCESSION   AB812049
VERSION     AB812049.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16588)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H711c"
                     /db_xref="taxon:562"
                     /note="serogroup:O110"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01526.1"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKHASLELHNK
                     PQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKEETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1081..1974
                     /gene="galF"
     CDS             1081..1974
                     /gene="galF"
                     /note="O110_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01527.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             2322..3413
                     /note="O110_02"
                     /gene="wfhE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01528.1"
                     /translation="MKKILHIQLMPILSGAQKVCLDEIENLGKNYEQWLLCSGVGPFT
                     KKASEFGVKHIVVDNLKREISIRDDLLALFKIYQNIKNKKFDIVHTHSSKTGFLGRVA
                     ARCAGVKTIVHTVHGFAFPSTENILLKIIYYVMEVIAALCTDALIVMNKSDYELAKKY
                     LPISNNKIYLLNNGVKLEKFKCKGDNYTNNDTIKIVMVGRLCEQKNPLLLLNAFCSLQ
                     DNYQLYYIGDGPLRTEIEKEILNKKLNERVKILGWVDNVPEVLPSFDIFVLPSRWEGM
                     PLAMLEAMASGLPVISSDIPSNRFLIENTNGELFKSEDAISLKEKIEYLGRDPKKRAL
                     ISQDAYKKIKANFSLDARICSLRRIYEEQ"
     CDS             3400..4476
                     /note="O110_03"
                     /gene="wdaW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01529.1"
                     /translation="MRSNKVILFIDSFKKGGAERVCSNYASILFNHNVDVEIWTYNIG
                     DKKMLQDICGKIKVHQFNSMNGTRCLLKIINKFRKETNAIFIAFNHQIALLLIMSRYV
                     TGKKNIIIARNVNYLSKDLYKDTSLKTKLVSFLMTKLYSKCDRFIAQCEAMKQDMINS
                     FNITPSKIKVIYNPLSPQIKKQRVSQECEINTDILFVGRLEKQKGIDKLVRIINYVLD
                     IEKDVTIKIIGNGSLVSLLKEIKRRPGTEIIHLPEVDNMAEHYQNSRMLILTSNYEGF
                     PNVLIEALGSGIPVISFDCPSGPAEIIDDGKNGFLIPVDDYDYYVDRIFFLLKNNLNS
                     YIPEKIENGEKDLLSLYNEVSNEN"
     CDS             4454..5461
                     /note="O110_04"
                     /gene="wfhF"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01530.1"
                     /translation="MRLAMKINIITVDGYFGQRLPTSKSLELQSLEQHLNLYGVKCEF
                     IDIQDVQNLRLDSDTRCIIGSHQNPGVKKYIDDVISIKFENYRDSLIPSVRNILAHEN
                     KGVQAIIMKECPDLFVQQNYYYRDDIEINKVKVLKRTYGAGSFGVALATDNKTFLKQK
                     KKMFYSDLKFDDIKFYIFECLKKYIFPSKLSKEHTIYFQRYNPYVLQDFIPDLNSDYK
                     VLVFGNKYYVLKRNVRDNDFRASGSGKFVFEDVSEKFLSFARKVIEFLHTPYASLDIV
                     ERGDGFGCIEFQCVHFGPYTQLNAEYYYEFKNGKWIREKNNYTLEQVYAEALVDFLKN
                     E"
     gene            5454..6704
                     /gene="wzx"
     CDS             5454..6704
                     /gene="wzx"
                     /note="O110_05"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01531.1"
                     /translation="MNNFITNILKLISGTAGGQLIALLLMPIVTRLVTVDEMGKYSLV
                     LAIIMVLSSVASMKLELAVMTLSSKTLVIHTLSAFCNVNFIIGILVLITGILLHFSIE
                     HFPLTAVDVVLTALALYFFSLAQFVSSYYSALDEFKKSAINRFAKPVLAVVFQIVLVN
                     LGFGENGLLLGFVIGYILSCFIFDSKFILMTMCHIDFRFVKESYKQFKSYFIFQAPAG
                     VINSLSQNVANFMLIGMIGAYSLGIYSLSFRMLQAPISLVSVSVRDAYFSRAKSIYEK
                     EPSKLANDMLYVIITLTILSGLVSAIIFSYLPFIFAQLFGNSWRESGVVASYLLPWFV
                     LLFSNPPASVVANIINMQRFIFIFEIANLIVRVLTLTFSWWFYKDFKLLIVHFSFAGV
                     IMNLLYILMVYKMAIEKCKVQHAK"
     CDS             6694..7848
                     /note="O110_06"
                     /gene="wfhG"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01532.1"
                     /translation="MQNNKIIIGGDVSFDQVKVDEYALSGLSKLSSFLGEAEYSIVNL
                     ECPITNSREKINKIGPNIKCGTNDGTKLLSALNLTHVTLANNHIMDYGYEGLKDTIEL
                     CNSLNIETFGAGINSEQASAIPAIILSNEKKIAIINACEKEFCISDLNKPGAAHCNVI
                     DMYHRITEAKKNNDEVVVILHGGHEHYKYVSPEYQKKLRFFAELGVSAIISHHTHFAS
                     GYEIWAGCPIFYSLGNLIFSRENYKDYWYHGYLVTLDFSEEKISFELTPYRQGKKNNL
                     FEFIAGDEKEKYLKDIHEINNVLQNRELLMDLWTKYVHKNEYAYFCWVNGLNRLQRMI
                     LKKIGIIRFIKFGLSNSKKQLRLWQLINCESHKEMTEKVLEVVNDDIRRN"
     gene            7829..9034
                     /gene="wzy"
     CDS             7829..9034
                     /gene="wzy"
                     /note="O110_07"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01533.1"
                     /translation="MTSEGTKTPLFSYMLSFSLLFIMSCESFNETRYLPVFFLLFITA
                     FYYIFFIRKISVSLLYSFGLVLSLVIILGALSSLPVLSYKQNLVNYGLSDFYISIVFK
                     LLVAFFILALCPNNLTLINIIKGVLIVHLSVFYVQAAIVYLTGYYIDLLYPVTGELQR
                     YESLFFLPWIGAVYRPTGLYVEPSTFSSFILWLLSIKILCQKNFCRFDIFVIVSSMLS
                     LSLAAIVYGAMFLLLALLCSGSIKLIKKIQFVLLLLPAPFVFYEIFKARLEALGGSGE
                     SLRENLNKLVFSQDPLTLLFGNGLLGLPAEVSDLNSGAELWRLGIAALNDNGLWLFFI
                     IKVGLVGLFILIIIMLLITRKKIDFIVFVILLLTKVTFFSFIFVFYFLTMVYLLLKRG
                     SNYEKNTIY"
     CDS             9012..10175
                     /note="O110_08"
                     /gene="wfhH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01534.1"
                     /translation="MKKILFIESHNGKISGAQKVTLNVAKLLSERGDDLTVMYPGSYL
                     DELALKYKSYAKAIKCYKMPSSLGSGGFDGLNVLQKIQVIIVSFLKMIFFYFSVSWYS
                     RKNKFDYIYAYDPRGLFLALPVAVLSKKKIIWHLHGKFLYGDFFFKIINFFCYRVLVP
                     SHAIKEALPNSKKINVLYNGFEFREMGNVPYKNSNVLKILFVGVFTPQKGVHLLLEAL
                     SLYKNKNKKIELTLVGDILDDDWKWYADLLNEKIKLLPENISICTPGWVKDVSLYLKL
                     GDYLIFPSQHSCELLINNVSRKFYGSEALPTVLIEAQAMNLPVIANDVPGVREIVQVN
                     GGFVVNMNSVDDILFIIERIAKEPRNMFTVKSEETRRKFSLDAMRKQLYCLIP"
     gene            10279..11685
                     /gene="gnd"
     CDS             10279..11685
                     /gene="gnd"
                     /note="O110_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01535.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFKDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKDGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            11934..13100
                     /gene="ugd"
     CDS             11934..13100
                     /gene="ugd"
                     /note="O110_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01536.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLTTKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(13166..14170)
                     /note="O110_11"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01537.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFVDERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRYNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            14522..15556
                     /gene="wzz"
     CDS             14522..15556
                     /gene="wzz"
                     /note="O110_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01538.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(15653..16264)
                     /gene="hisI"
     CDS             complement(15653..16264)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01539.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4986 a   2902 c   3540 g   5160 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaac
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tctccgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac
      181 tggggcatcg gcataggtct tgcgggaagt acctacgata acaactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataac
      541 acccatcttg cttataaatt gcgcggtatc caaatctccg ccgggaatgc tgtctccttt
      601 gtggcactga ctaacattga ggtgaagcat gcgtcgcttg agctacacaa taagccgcaa
      661 catcttttta tgcgaaatat caaagtgatg caggaatcct ctgttggacc cgcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaga agaaacactg
      781 ctgtctcttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggaga
      901 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcaa ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
     1141 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
     1321 gtgaagcgtc agctgctggc ggaagtgcag tccatctgtc cgccgggtgt gaccattatg
     1381 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttttatgtgc acgacccgcc
     1441 attggtgaca acccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc
     1501 gacccgctgc gctacaacct tgctgccatg attgcgcgct tcaacgaaac aggacgcagc
     1561 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actccgtcat tcagaccaaa
     1621 gaaccgctgg atcgcgaagg caaagtcagc cgcatcgtgg aatttatcga aaaaccggat
     1681 cagccgcaga cgctggactc agacatcatg gccgtgggtc gctatgtgct ttctgccgat
     1741 atttggccgg aacttgaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat
     1801 gccatcgctg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac aggtgacagc
     1861 tacgactgcg gtaaaaaaat gggttatatg caggcatttg tgaagtatgg actgcgcaac
     1921 ctgaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa
     1981 tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
     2041 gtaatttgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt
     2101 tagcaatagg attttagtca aagttttcca gggttttcct tgtttccaga gcggattggt
     2161 aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt gggtaacgct cgctacatcg
     2221 taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt ggttgttgtg
     2281 gaattaatta atagcgagtt tgtgtttgag attttattaa aatgaaaaaa attctacata
     2341 tacaattgat gccgattctt agtggtgcgc aaaaggtttg cttggatgaa attgagaatt
     2401 taggaaagaa ctatgaacaa tggttgcttt gctcgggtgt tggacctttt actaaaaagg
     2461 catctgaatt tggagtcaaa catatagtag tagataatct taaaagagag atatcgatta
     2521 gagatgattt attggctctt tttaagatat atcaaaacat taaaaacaag aagtttgata
     2581 ttgttcatac tcactcttct aagactggtt ttttaggtag ggtcgcagcg aggtgtgccg
     2641 gtgtaaaaac catagtacat actgtacatg gttttgcatt tccctcaacg gaaaatatac
     2701 tcttaaaaat aatatattat gtgatggagg ttatagcagc tttatgtact gatgctttaa
     2761 ttgttatgaa taaaagtgat tacgaattag caaaaaaata tttaccaata tcgaataata
     2821 aaatatatct tcttaataac ggtgtaaaac tagaaaaatt taagtgtaag ggagataatt
     2881 acacaaataa tgacacaatt aaaatcgtga tggttgggcg tttatgtgag caaaaaaacc
     2941 cattgttatt actgaatgca ttttgttctc tgcaggataa ttatcaattg tattatatag
     3001 gtgatggacc attacgaact gaaatagaaa aagaaatttt aaataaaaaa ttaaatgaga
     3061 gagtcaaaat attggggtgg gtagataatg tacccgaagt attgccatct tttgatattt
     3121 ttgttttgcc atcaagatgg gaaggtatgc cattggcaat gcttgaagct atggcatcag
     3181 gcttgcctgt gatttcgtca gatattccat ctaatagatt tttaattgaa aatactaatg
     3241 gtgagttatt taaaagtgaa gatgctatct ctttaaaaga aaaaatagaa tatttgggga
     3301 gagatcccaa gaaaagagcg ttgatttccc aagatgccta taaaaaaata aaagccaatt
     3361 tcagtttgga cgcaagaata tgctctttga ggagaatata tgaggagcaa taaagtcatt
     3421 ttgtttattg attcatttaa gaaagggggg gctgaacgtg tttgttcaaa ttatgcatca
     3481 atacttttta atcataatgt tgatgtagaa atatggactt ataatatagg tgacaaaaaa
     3541 atgctgcagg atatatgcgg caaaataaaa gttcatcaat ttaattctat gaatgggaca
     3601 agatgtttat taaaaataat aaataaattt cgtaaggaaa ctaatgcaat atttatcgca
     3661 tttaatcatc aaatagcact tttattaatt atgtcaaggt atgtaacggg gaaaaaaaat
     3721 attatcatag ctcgtaatgt aaattactta agtaaagatc tttataaaga tacttcgtta
     3781 aaaactaagc ttgtgtcttt tttgatgacc aaactgtaca gtaagtgcga tcgttttatt
     3841 gctcaatgcg aggcaatgaa acaagatatg attaattctt ttaatattac cccatcaaaa
     3901 ataaaagtaa tatataatcc tttatctcct caaattaaaa aacaaagagt ttctcaagag
     3961 tgcgaaataa atacggatat actatttgtc ggtcgacttg aaaagcaaaa aggaattgat
     4021 aaattagtaa ggatcattaa ctatgtactt gatatcgaaa aagatgtgac gataaaaata
     4081 ataggaaatg gttcgttagt atcgctttta aaagaaataa aacgtagacc gggaactgaa
     4141 ataattcatt tgcctgaagt tgataatatg gcagagcatt accaaaattc gcgaatgctt
     4201 attttaacgt caaattatga agggtttcct aatgtattaa tcgaagcttt aggaagtggt
     4261 atacctgtta tcagttttga ttgtccctct gggccagcgg agattataga tgatggtaag
     4321 aatggttttt taattcctgt tgatgattat gattattatg ttgatagaat ttttttcctt
     4381 ttaaaaaata atctgaatag ttatatccca gaaaagattg agaatggtga aaaagatttg
     4441 ttaagtttat ataatgaggt tagcaatgaa aattaatatt attactgtgg atggatattt
     4501 tggacaaaga ttaccgacat caaaaagcct ggaactacaa tctttagagc aacacttgaa
     4561 tttatatgga gtaaaatgtg aattcattga tattcaagat gttcagaatt tacgtttaga
     4621 tagtgatact cgctgtatta taggtagtca tcaaaatccg ggtgtaaaaa aatatataga
     4681 tgacgtaata tctataaaat ttgaaaatta tagagacagt ttaataccaa gtgtgcgaaa
     4741 tatattagcg catgaaaaca aaggtgtgca agctattata atgaaagaat gccctgattt
     4801 atttgttcaa caaaattatt attatcgtga tgacatagaa ataaataaag taaaagtact
     4861 gaaacggacg tatggagcag ggagttttgg ggttgcatta gctacagata ataaaacttt
     4921 tttaaaacaa aaaaagaaaa tgttttattc tgatttgaaa tttgatgata ttaagtttta
     4981 tatatttgaa tgtttaaaga aatatatatt tccctcaaaa cttagtaaag aacacactat
     5041 ttattttcaa agatacaatc cttacgtttt acaagatttt attcctgatt tgaatagtga
     5101 ttataaggtg ttagtatttg gcaataaata ttatgttctt aaaaggaatg ttcgggataa
     5161 tgactttcgt gcaagtggaa gtggaaaatt tgtatttgag gatgtttctg aaaagtttct
     5221 ctcatttgcc agaaaagtaa tagaattttt gcacactcca tatgcatctc ttgatatagt
     5281 agaaaggggg gatggatttg gctgtattga atttcagtgc gtacattttg gaccatatac
     5341 tcaacttaat gcggagtatt attatgaatt taaaaatggt aagtggatta gagaaaaaaa
     5401 taattatact ttagagcagg tctatgctga agcattagtg gattttttaa aaaatgaata
     5461 attttataac aaatatcctt aaattaatat ctggtactgc tggtgggcaa cttatcgcat
     5521 tgttattgat gcctattgta acaaggctgg ttacagtaga tgagatgggg aagtattcac
     5581 ttgtactggc tataattatg gtgctaagta gtgttgcaag catgaaactt gagcttgccg
     5641 ttatgacttt atcatcaaag acgcttgtca ttcatacatt gtctgctttt tgtaatgtca
     5701 actttataat tggtattctg gtacttatta caggtatttt gttgcatttt tccattgagc
     5761 attttccatt gacagcggtt gatgtagtcc ttaccgcgtt agctttgtat ttcttttctc
     5821 ttgcacaatt tgtgtcatcc tattattcag cacttgatga gttcaaaaaa agcgcaataa
     5881 acagatttgc caaacctgta ttagctgttg tttttcagat agtattagta aatttaggat
     5941 ttggcgaaaa tggcttatta ttaggatttg ttataggtta tatactaagt tgttttatat
     6001 ttgattctaa attcatattg atgacgatgt gtcatattga ttttaggttc gtgaaagagt
     6061 cttacaaaca gtttaaatca tattttattt ttcaggcacc agctggtgtt attaattcat
     6121 tatcacaaaa tgtcgcaaat tttatgctaa tcggtatgat aggagcctat tcgcttggta
     6181 tttattcact ttcgtttcgc atgttacaag cacctatttc cttggtaagt gtttctgtgc
     6241 gcgatgcata tttctcacgt gcaaaatcga tatatgagaa agaaccatcg aaacttgcaa
     6301 atgacatgtt atatgttatt attactttga caatattatc ggggttggtc tccgcgatta
     6361 ttttttcata tcttcctttt attttcgcac aactatttgg aaattcttgg agagagtcag
     6421 gtgtcgtggc atcatacctc ctcccttggt ttgttctgtt attttccaat cccccagctt
     6481 cagtagttgc taatataata aacatgcaga ggtttatatt tatatttgag atcgcaaatc
     6541 taattgttcg ggtattaaca ttaacatttt cttggtggtt ttataaagat tttaaactgt
     6601 taatagttca ttttagtttt gccggagtaa ttatgaattt gctatatatt ctgatggttt
     6661 ataaaatggc tattgaaaaa tgcaaggtac aacatgcaaa ataacaaaat cattattggt
     6721 ggtgatgttt cttttgatca agttaaagtt gacgagtacg cattaagtgg attgagtaaa
     6781 ttgtcttcat ttctcggtga ggcggaatat tctattgtaa atttagaatg tcctattacc
     6841 aatagccgag aaaaaattaa caagattggt cccaatataa agtgtgggac taatgatgga
     6901 acgaaattat tatcagcatt gaacttgact catgtaaccc ttgcaaataa tcatattatg
     6961 gattatggat atgaggggtt gaaagataca atagaactat gtaattcatt aaatattgaa
     7021 acttttgggg ctggaattaa ctcagagcag gctagcgcaa taccagcgat aatattatcc
     7081 aatgaaaaaa agattgctat aataaatgct tgcgaaaaag aattttgtat ttcagatcta
     7141 aataagcctg gagctgcgca ttgtaatgta atcgatatgt atcaccgtat tacagaagct
     7201 aagaaaaata atgatgaggt agtagttatt ttacacggtg gacatgagca ttataaatat
     7261 gttagtccag agtatcaaaa gaaacttcgt ttttttgctg agttgggcgt gtcggctatt
     7321 atatctcatc atacacactt tgcctctgga tatgaaatat gggcaggatg tcctatattt
     7381 tatagcttgg gtaatttgat tttttctcgc gaaaattata aagattattg gtaccatgga
     7441 taccttgtaa ctttagattt ttctgaagaa aaaatatcat ttgaacttac tccatacagg
     7501 caaggtaaaa aaaataatct atttgagttt atagctgggg atgaaaaaga aaaatattta
     7561 aaagatatac atgaaataaa taatgttttg caaaatagag agttattaat ggatttatgg
     7621 acgaaatatg ttcataaaaa tgaatatgct tatttttgtt gggttaatgg tttaaatcgt
     7681 ttgcagcgca tgattttgaa aaaaattgga attattagat ttattaaatt tggactcagt
     7741 aatagtaaaa aacagctaag actttggcaa ttgattaatt gtgagtcaca taaagaaatg
     7801 acggaaaaag tgttagaggt agtgaatgat gacatcagaa ggaactaaaa ctccactttt
     7861 ttcgtatatg ctttcctttt cattgttatt tataatgtct tgtgaaagtt ttaatgaaac
     7921 aagatatctt cccgtatttt ttcttttgtt tatcactgca ttttattaca ttttctttat
     7981 aaggaagatc tctgtatctc ttttatattc cttcggttta gtattgtctc tggtcattat
     8041 tttgggagcg ttgtcttcat tgccagtatt gtcgtataaa caaaatttgg tcaattatgg
     8101 actgtcggat ttttatatat ctattgtttt taagttgctg gtagcatttt ttatattggc
     8161 tctctgtcca aataatttaa cattaataaa catcatcaaa ggggttttga ttgttcattt
     8221 aagtgttttt tatgtacagg cggctattgt ctacttaaca ggttactata tagatttgtt
     8281 atatcctgtt acgggagagt tacaacgtta tgaaagtctt ttctttttgc cttggatagg
     8341 agcggtttat agacctacgg ggttgtatgt tgaaccctca actttttctt cttttatttt
     8401 atggttgctt tcaataaaaa ttttatgcca gaaaaatttt tgcagatttg atatatttgt
     8461 gatcgtttca tcaatgctct cgctatcatt ggctgcaatt gtttatggtg caatgttcct
     8521 tttacttgct ttactatgtt ctggttcgat taaactaatt aaaaaaattc aatttgtttt
     8581 attgttactt cctgctccat ttgtatttta tgaaattttc aaagctcgcc ttgaggcatt
     8641 aggaggaagt ggtgaaagtt tgagggaaaa tttaaataaa ttggtttttt ctcaagatcc
     8701 attaacattg ctatttggta atggtttact gggattaccg gccgaggtaa gtgacttaaa
     8761 ttcaggtgct gaactctggc gtttaggtat cgctgctctg aatgataacg ggctttggtt
     8821 gttttttatc ataaaagtag gattggttgg tttatttata ctaataatta taatgttact
     8881 cattacacga aaaaaaatag attttatagt gttcgtcata ttattactga cgaaggttac
     8941 tttttttagc tttatatttg ttttttattt tttgacaatg gtttatttat tattgaagcg
     9001 cggttctaac tatgaaaaaa atactattta ttgaatccca taacggtaaa atatccggcg
     9061 cacagaaggt gacattaaat gtggctaaac ttctgtctga aaggggtgat gatttaacag
     9121 ttatgtatcc agggagctat ttagacgaat tggctttgaa atacaaatct tatgcaaaag
     9181 ctattaaatg ctacaaaatg ccaagtagtt taggtagtgg aggatttgat gggttaaatg
     9241 tgttgcaaaa aatacaagta atcattgttt cttttttgaa aatgattttt ttttatttta
     9301 gtgtttcctg gtattcaagg aagaataaat ttgattacat ttatgcatat gacccaagag
     9361 gactatttct agcattgcct gtagcagtgc tctctaaaaa aaagattata tggcatctcc
     9421 atggaaaatt tttatatggt gacttctttt ttaaaattat aaattttttt tgctatagag
     9481 tcttagtccc atctcacgct attaaagaag cattacctaa ttcgaaaaaa attaatgttc
     9541 tttataatgg ttttgaattt agagaaatgg gtaatgttcc atataaaaat agtaatgtgc
     9601 ttaagatttt atttgtcggg gtttttacac cgcaaaaggg agtgcattta cttttggagg
     9661 cactttcact ttataaaaat aaaaataaaa aaattgaatt aacccttgta ggggatatat
     9721 tagatgatga ttggaaatgg tacgctgatt tgctaaatga aaaaattaaa cttttgcctg
     9781 aaaatatttc tatatgtaca ccaggctggg taaaagatgt ctctttgtat ttaaaattgg
     9841 gtgattattt gatatttcct tctcaacatt catgtgaatt gttaataaac aatgtttctc
     9901 gtaagttcta tggcagtgaa gcattaccaa ccgtgttaat tgaagctcaa gccatgaatt
     9961 taccagtaat tgcaaatgat gtgcctgggg tcagagaaat tgtacaagtg aatggagggt
    10021 tcgtggttaa tatgaatagt gttgatgata tactatttat tattgaaagg attgctaaag
    10081 agccaagaaa tatgtttact gtcaaatcag aggaaaccag acgtaagttt tcattagatg
    10141 ctatgcgaaa gcaattatat tgtttaatac catgaaatat cataccatat ccatatatgg
    10201 taattttact ctaacatact tttaatcagc aaaagccgtg acaacattca cggtaatccc
    10261 tgcagacagg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat ggccgtgatg
    10321 gggcgcaacc ttgcgctcaa catcgaaagc cgtggttata ccgtctctat tttcaaccgt
    10381 tcccgtgaaa agacggaaga agtgattgcc gaaaatccag gcaagaaact ggttccttac
    10441 tatacggtga aagagtttgt tgaatctctg gaaacgcctc gtcgcatcct gttaatggtg
    10501 aaagcaggtg caggcacgga tgctgctatt gattccctca agccatacct cgataaaggt
    10561 gacatcatta ttgatggtgg taataccttc ttcaaggaca ctattcgtcg taatcgtgag
    10621 ctatctgcag aaggctttaa cttcattggt accggtgttt ccggaggcga agaaggcgcg
    10681 ttgaaaggtc cttccattat gcctggtggc cagaaagaag cctatgaact ggttgcacca
    10741 atcctgacca aaatcgccgc agtagctgaa gacggtgagc catgcgttac ctatattggg
    10801 gccgatggtg caggtcacta tgtgaagatg gttcacaacg gtattgaata cggcgatatg
    10861 cagctgattg ctgaagccta ttctctgctt aaagatggcc tgaacctcac caacgaagaa
    10921 ctggcacaga cctttaccga gtggaataac ggtgaactga gcagctacct gatcgacatc
    10981 accaaagata tcttcaccaa aaaagatgaa gacggtaact acctggttga tgtgattctg
    11041 gatgaagcag caaacaaagg tacgggcaaa tggaccagcc agagtgcgct tgatctcggc
    11101 gaaccgctgt cgctgattac cgagtctgtg tttgcacgtt atatctcttc tttgaaagat
    11161 cagcgtgttg ccgcatctaa agttctctct ggcccgcaag cacagccagc aggcgacaag
    11221 gctgagttca tcgaaaaagt tcgccgtgcg ctgtatctgg gcaagatcgt ttcttacgct
    11281 cagggcttct ctcagctacg cgccgcgtct gaagagtaca attgggatct gaactatggt
    11341 gaaatcgcga agattttccg tgcaggttgc atcatccgtg cgcagttcct gcagaaaatc
    11401 accgatgcgt atgccgaaaa tccgcagatc gctaacctgc tgctggctcc gtactttaaa
    11461 caaattgccg atgattacca gcaggcgctg cgtgatgtcg ttgcttatgc ggtacagaac
    11521 ggtatcccgg ttccgacctt cgccgctgcg gttgcatatt atgacagcta ccgttccgct
    11581 gttctgcctg cgaacctgat ccaggcacag cgtgactatt tcggtgcgca tacttataag
    11641 cgcattgata aagaaggtgt gttccatact gaatggctgg attaatctga tttaaatcaa
    11701 ttaaccaaca caaggcccgg agaaaccctc cgggcttttt tattatacaa agcggcaggt
    11761 tatggtcttt ttttataatt tatgattaaa aaagcgatat aatacagcgc cgcacagcag
    11821 gatcgctgcc ttgacagttc atatacatca gcgttaaaaa tcccgcagta gatgaagttg
    11881 tggtggtgga ttaatgacca ctctaaatgt ttaaccggaa gaagtcagag ctaatgaaaa
    11941 taacaatttc aggaacaggt tatgttggtc tttcaaatgg tattctgatt gcgcaaaacc
    12001 acgaagtggt tgcactggat atcgttcagg ccaaagtgga catgcttaac aagaagcagt
    12061 caccgattgt tgataaggag attgaagagt atctgacgac taaagatctc aatttccgcg
    12121 ctacgacaga taagtatgac gcgtataaaa atgccgatta cgttattatt gccacaccta
    12181 ccgattatga tccgaaaacg aattatttta atacctcaag cgtggaagcg gtcattcgcg
    12241 atgtgacaga aattaatccc aacgcggtaa tgattataaa atcaactatc cctgttggtt
    12301 ttacagagtc cattaaagaa cgttttggta ttgaaaatgt gatcttttcg cctgagtttt
    12361 tgcgtgaagg taaagcactt tatgataact tacacccatc acgcattgtg attggcgagc
    12421 agtctgaacg cgctgaacgt tttgctgcgt tattacagga aggcgctatt aagcaagaca
    12481 taccaacatt gtttactgac tcaaccgaag ctgaggcgat taaacttttt gccaacactt
    12541 atctggctat gcgtgtagcg tatttcaatg aacttgatag ttatgctgaa agcctggggc
    12601 ttaattcacg ccagattatt gagggcgtat gccttgaccc gcgtatcggt aatcactaca
    12661 acaacccgtc attcggttat ggtggttatt gtctgccgaa agataccaag cagttactag
    12721 caaattacca gtctgtgccg aataacctga tctcggcaat tgttgacgcc aaccgcacgc
    12781 gcaaagattt tattgccgat tctatccttg cacgtaaacc gaaagttgtt ggcgtctatc
    12841 gtttgattat gaagagtggt tcagacaatt tccgtgcttc ctcgattcag ggtattatga
    12901 agcgaatcaa agcgaaaggc gtgcctgtaa tcgtttatga gccagctatg aaagaggacg
    12961 attttttccg gtcgcgcgtg gtacgtgatc tggatgcgtt caaacaagaa gctgatgtta
    13021 ttatttctaa ccgtatgtct gccgatctgg ctgatgtagc agataaagtt tatacgcgcg
    13081 acttgtttgg caatgattaa ttattttgtt ttattctaag aaaaggccct aatatattag
    13141 ggccttttct tatggttttg taaaatcaaa ctttatagaa gttacgatac cattctacaa
    13201 agttctttac cccctcttta actgacgttt caggtttgaa tcctattacg tcatacagtg
    13261 cttttgtatc agcactggtt tccagtacat caccgggttg gagaggcatc atattcttgt
    13321 tggcttcaat acccagagcc tcttctaagg cattgatata gtccatcaac tccacaggcg
    13381 aactattacc aatgttatag acacgatatg gtgctgaact tgttgctggc gagcctgttt
    13441 ctacagccca gtgtggatct ttttctggaa taacatcctg taagcgaata atagcttcgg
    13501 caatatcatc aatataagta aagtcacgct tcattttgcc gaagttgtaa acatcaatgc
    13561 ttttaccttc cagcatggct ttagtaaatt taaataatgc catatccgga cgtccccatg
    13621 gaccataaac cgtaaagaaa cgcagccctg tggtcggtaa gccatacaaa tgagaatacg
    13681 tatgggccat gagttcattc gcttttttag ttgctgcata aagcgaaaca ggatgatcta
    13741 cagagtcatc tgtagagaaa ggcatcttgc ggttcatgcc ataaacagaa ctggaggaag
    13801 cgtaaagtag atgctgaaca ttattatagc ggcatccttc cagtatgttc aggaatccaa
    13861 tcaggtttgc atctgcatat gcattgggat tttcaaggga gtaacgtaca cctgcttgcg
    13921 cagcgaggtt tattacgcgt tcgaaccgct cgtctacaaa cagtgccgcc attttctcgc
    13981 gatcggccag gtcaatttta taaaaactga aattgtcgtg cttgagtaaa tcaagtcgtg
    14041 cttgtttgag gttgacatcg taataatcat ttaagttgtc aatgcctaca acctgatgac
    14101 cagctgcaag aagccgttta cttacataga aaccgataaa gccagcagct cccgtaacca
    14161 gaaatttcat ttataatcct cgctcaggct agaatatagc caattttcat ctagcataac
    14221 tgaaagttaa atcataccgt tagacaagaa aaaaagataa tcggtatcag ttctaaacct
    14281 ggctgttttt tctggtaacg tgctcatttt acaatcaaag ctgttctaag ctgactatac
    14341 aagccgacgt cattatctcc aaccgtatgg cagaagagct taaggatgtg gcagataagg
    14401 tctacacccg cgatctcttt ggcagcgact aacatcctgt tatcagggcg attttcgccc
    14461 tgattctctt atgttccctt tgtaataatt cattattttt atcatttatc ctatagcatt
    14521 catggcgatt atcgctaaac tatggcgact taaaattatt tcgtccgtta gggtaatgat
    14581 gagagtagaa aataataatg tttctgggca aaaccatgac ccggaacaga ttgatttgat
    14641 tgatttactg gtgcagttgt ggcgtggcaa gatgacaatt atcatctctg tcattgttgc
    14701 tattgccctg gctatagggt atctggccgt tgccaaagaa aagtggactt ccacagctat
    14761 cgttactcaa cctgatgtgg gacaaatcgc tggttataac aatgccataa atgtgattta
    14821 tggctcggca gcgccaaaag tgtctgagat ccagtcgatg ttaattggtc gtttcagcac
    14881 gacattttct gcattagcag aaacgctgga taatcaggaa gaacctgaaa aactgaccat
    14941 tgagcctacc gttaaaaatc agtcattacc tttagcggtt tcttatgtgg gtgaaacacc
    15001 tgaaggtgcg cagaaacagc tggcgcagta tatccagcaa gttgacgacc aggtaaatga
    15061 agaactggaa caagacctaa aagacaacat cgcgctgcaa atgaaaaact tgcaggattc
    15121 tctgaagacg caggaagtgg tcgctcaaga acagaaagat ctgcgtatcc gtcagattca
    15181 ggaagcgttg cagtatgcga atcaggcgca ggtgacaaag ccgcagattc agcagacaca
    15241 ggacgtaacc caggatacta tgttcctttt ggggagcgaa gcgctggagt cgatgattaa
    15301 gcacgaggcg actcgtccgt tggtgttctc atcaaactac tatcagactc gtcaaaacct
    15361 gctggatatc gacaatcttg acgttgataa acttgatatt catgcttacc gctatgtaat
    15421 gaaaccgacg ttacctattc gtcgcgatag cccgaaaaag gcaattacct tgattctggc
    15481 ggtgctgctg ggcggcatgg ttggcgcggg gattgtgttg gggcgtaacg ctctgcgtaa
    15541 ttacaacgcg aagtaatctt tgcagcttta aagaaaacgg gcagggtggt gacaccttgc
    15601 ccgttttttt tgccggatgc gacaacaaca tcgcatccgc ttaccccgca actcactgat
    15661 gccgtttgcg caggttctca atcaccgtcg ttaagtccag atcctgatcc tgcaacaata
    15721 ccagcaggtg atacatcaaa tcagatgcct cgttggtcag ctcaaagcgg tcatgtaccg
    15781 ttgctgccag tgcggtttcc acgccttctt cgcccacttt ttgcgcaatg cgtttggtgc
    15841 cgctggcata cagtttggca gtgtaagagg tttccgggtc ggcagatttg cgttcggcga
    15901 gcaattgttc cagttgatac aggaacagcc attggtgagc ggtgtcgccg aagcagctgc
    15961 tggtgccttt gtggcaggtc ggaccgatgg gattagccag cactaataaa gtgtcgttgt
    16021 cgcagtccgg tgcaatactc accacattga ggaaattgcc cgacgtttcg cctttggtcc
    16081 acagtcgctg tttagtgcgc gagaagaagg tgactttgcc gctttcgatg gttttgtcta
    16141 aggcttccgg gttcatatag cccagcatta acacttcgcc ggataccgcg tgttgcacaa
    16201 tcaccggcat cagtccgtcg gttttttccc agtccagttc gcgacgttgt tgttctgtta
    16261 acatatcctg atctccacgc cctgtgttgc caggtacgct tttaattcac caatattgat
    16321 tatttgtttg tggaatacgg aagctgccag cgcgccgtca acgtcggcat cacggaaggc
    16381 ttcgaggaag tgttccatgg tgcccgcgcc accggaggca attagcggga cgtggcaaac
    16441 ttcacgtact tttttcagtt gttcgaggtc gtaaccgtta cgcacgccgt cctgattcat
    16501 catgttgagg acgatttctc cggcaccgcg tttttgcact tcctgtaccc agtcgagcgt
    16561 ttcccactga gtgacgcggg tgcggctt
//
