LOCUS       AF078736               14516 bp    DNA     linear   BCT 03-AUG-1999
DEFINITION  Escherichia coli O111 O antigen gene cluster, partial sequence.
ACCESSION   AF078736 U13629
VERSION     AF078736.1  GI:5678713
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14516)
  AUTHORS   Bastin,D.A. and Reeves,P.R.
  TITLE     Sequence and analysis of the O antigen gene (rfb) cluster of
            Escherichia coli O111
  JOURNAL   Gene 164 (1), 17-23 (1995)
   PUBMED   7590310
REFERENCE   2  (bases 3021 to 9981)
  AUTHORS   Reeves,P.R., Hobbs,M., Valvano,M.A., Skurnik,M., Whitfield,C.,
            Coplin,D., Kido,N., Klena,J., Maskell,D., Raetz,C.R. and Rick,P.D.
  TITLE     Bacterial polysaccharide synthesis and gene nomenclature
  JOURNAL   Trends Microbiol. 4 (12), 495-503 (1996)
   PUBMED   9004408
REFERENCE   3  (bases 1 to 14516)
  AUTHORS   Wang,L., Curd,H., Qu,W. and Reeves,P.R.
  TITLE     Sequencing of Escherichia coli O111 O-antigen gene cluster and
            identification of O111-specific genes
  JOURNAL   J. Clin. Microbiol. 36 (11), 3182-3187 (1998)
   PUBMED   9774562
REFERENCE   4  (bases 3021 to 9981)
  AUTHORS   Bastin,D.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1997) Microbiology, University of Sydney, Maze
            Crescent, Sydney, NSW 2006, Australia
REFERENCE   5  (bases 1 to 14516)
  AUTHORS   Wang,L., Curd,H., Qu,W., Bastin,D.A. and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-1998) Microbiology, University of Sydney, Maze
            Crescent, Sydney, NSW 2006, Australia
COMMENT     On Aug 3, 1999 this sequence version replaced gi:1155016.
FEATURES             Location/Qualifiers
     source          1..14516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Stoke W; M92"
                     /serotype="O111"
                     /db_xref="taxon:562"
     gene            <1..273
                     /gene="galF"
     CDS             <1..273
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glucose-1-phosphate
                     uridylyltransferase"
                     /protein_id="AAD46727.1"
                     /db_xref="GI:5678714"
                     /translation="DLMAVGRYVLSADIWAELEKTAPGAWGRIQLTDAIAELAKKQSV
                     DAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKSIKKLLSE"
     gene            739..1932
                     /gene="wbdH"
     CDS             739..1932
                     /gene="wbdH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAD46728.1"
                     /db_xref="GI:5678715"
                     /translation="MLLCCIHINVYYLLLECDMKKIVIIGNVASMMLRFRKELIMNLV
                     RQGDNVYCLANDFSTEDLKVLSSWGVKGVKFSLNSKGINPFKDIIAVYELKKILKDIS
                     PDIVFSYFVKPVIFGTIASKLSKVPRIVGMIEGLGNAFTYYKGKQTTKTKMIKWIQIL
                     LYKLALPMLDDLILLNHDDKKDLIDQYNIKAKVTVLGGIGLDLNEFSYKEPPKEKITF
                     IFIARLLREKGIFEFIEAAKFVKTTYPSSEFVILGGFESNNPFSLQKNEIESLRKEHD
                     LIYPGHVENVQDWLEKSSVFVLPTSYREGVPRVIQEAMAIGRPVITTNVPGCRDIIND
                     GVNGFLIPPFEINLLAEKMKYFIENKDKVLEMGLAGRKFAEKNFDAFEKNNRLASIIK
                     SNNDF"
     gene            1983..3101
                     /gene="gmd"
     CDS             1983..3101
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-D-mannose dehydratase"
                     /protein_id="AAD46729.1"
                     /db_xref="GI:5678716"
                     /translation="MNKVALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDSHLANPKLFLHYGDLTDTSNLTRILKEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRILGLEKKTKFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMFACNGILFNHESPRRGETFVTRKITRGIANIAQGLDKCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQETPEDFVIATGIQYSVREFVTMAAEQVGIELAF
                     EGEGVNEKGVVVSVNGTDAKAVNPGDVIISVDPRYFRPAEVETLLGDPTNAHKKLGWS
                     PEITLREMVKEMVSSDLAIAKKNVLLKANNIATNIPQE"
     gene            3174..3623
                     /gene="wbdI"
     CDS             3174..3623
                     /gene="wbdI"
                     /note="previously known as orf3.4"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdI"
                     /protein_id="AAC44881.1"
                     /db_xref="GI:1155017"
                     /translation="MFITSDKFREIIKLVPLVSIDLLIENENGEYLFGLRNNRPAKNY
                     FFVPGGRIRKNESIKNAFKRISSMELGKEYGISGSVFNGVWEHFYDDGFFSEGEATHY
                     IVLCYTLKVLKSELNLPDDQHREYLWLTKHQINAKQDVHNYSKNYFL"
     gene            3657..5108
                     /gene="manC"
     CDS             3657..5108
                     /gene="manC"
                     /EC_number="2.7.7.22"
                     /note="previously known as RfbM"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AAC44882.1"
                     /db_xref="GI:1155018"
                     /translation="MSQCLYPVIIAGGTGSRLWPLSRVLYPKQFLNLVGDSTMLQTTI
                     TRLDGIECENPIVICNEDHRFIVAEQLRQIGKLTKNIILEPKGRNTAPAIALAAFIAQ
                     KNNPNDDPLLLVLAADHSINNEKAFRESIIKAMPYATSGKLVTFGIIPDTANTGYGYI
                     KRSSSADPNKEFPAYNVAEFVEKPDVKTAQEYISSGNYYWNSGMFLFRASKYLDELRK
                     FRPDIYHSCECATATANIDMDFVRINEAEFINCPEESIDYAVMEKTKDAVVLPIDIGW
                     NDVGSWSSLWDISQKDCHGNVCHGDVLNHDGENSFIYSESSLVATVGVSNLVIVQTKD
                     AVLVADRDKVQNVKNIVDDLKKRKRAEYYMHRAVFRPWGKFDAIDQGDRYRVKKIIVK
                     PGEGLDLRMHHHRAEHWIVVSGTAKVSLGSEVKLLVSNESIYIPQGAKYSLENPGVIP
                     LHLIEVSSGDYLESDDIVRFTDRYNSKQFLKRD"
     gene            5114..6484
                     /gene="manB"
     CDS             5114..6484
                     /gene="manB"
                     /EC_number="5.4.2.8"
                     /note="previously known as RfbK"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AAC44883.1"
                     /db_xref="GI:1155019"
                     /translation="MNKITCFKAYDIRGRLGAELNDEIAYRIGRAYGEFFKPQTVVVG
                     GDARLTSESLKKSLSNGLCDAGVNVLDLGMCGTEEIYFSTWYLGIDGGIEVTASHNPI
                     DYNGMKLVTKGARPISSDTGLKDIQQLVESNNFEELNLEKKGNITKYSTRDAYINHLM
                     GYANLQKIKKIKIVVNSGNGAAGPVIDAIEECFLRNNIPIQFVKINNTPDGNFPHGIP
                     NPLLPECREDTSSAVIRHSADFGIAFDGDFDRCFFFDENGQFIEGYYIVGLLAEVFLG
                     KYPNAKIIHDPRLIWNTIDIVESHGGIPIMTKTGHAYIKQRMREEDAVYGGEMSAHHY
                     FKDFAYCDSGMIPWILICELLSLTNKKLGELVCGCINDWPASGEINCTLDNPQNEIDK
                     LFNRYKDSALAVDYTDGLTMEFSDWRFNVRCSNTEPVVRLNVESRNNAILMQEKTEEI
                     LNFISK"
     gene            6521..7444
                     /gene="wbdJ"
     CDS             6521..7444
                     /gene="wbdJ"
                     /note="previously known as orf6.7"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdJ"
                     /protein_id="AAC44884.1"
                     /db_xref="GI:1155020"
                     /translation="MKVLLTGSTGMVGKNILEHDSASKYNILTPTSSDLNLLDKNEIE
                     KFMLINMPDCIIHAAGLVGGIHANISRPFDFLEKNLQMGLNLVSVAKKLGIKKVLNLG
                     SSCMYPKNFEEAIPEKALLTGELEETNEGYAIAKIAVAKACEYISRENSNYFYKTIIP
                     CNLYGKYDKFDDNSSHMIPAVIKKIHHAKINNVPEIEIWGDGNSRREFMYAEDLADLI
                     FYVIPKIEFMPNMVNAGLGYDYSINDYYKIIAEEIGYTGSFSHDLTKPTGMKRKLVDI
                     SLLNKIGWSSHFELRDGIRKTYNYYLENQNK"
     gene            7441..8607
                     /gene="wbdK"
     CDS             7441..8607
                     /gene="wbdK"
                     /note="previously known as orf7.7"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdK"
                     /protein_id="AAC44885.1"
                     /db_xref="GI:1155021"
                     /translation="MITYPLASNTWDEYEYAAIQSVIDSKMFTMGKKVELYEKNFADL
                     FGSKYAVMVSSGSTANLLMIAALFFTNKPKLKRGDEIIVPAVSWSTTYYPLQQYGLKV
                     KFVDINKETLNIDIDSLKNAISDKTKAILTVNLLGNPNDFAKINEIINNRDIILLEDN
                     CESMGAVFQNKQAGTFGVMGTFSSFYSHHIATMEGGCVVTDDEELYHVLLCLRAHGWT
                     RNLPKENMVTGTKSDDIFEESFKFVLPGYNVRPLEMSGAIGIEQLKKLPGFISTRRSN
                     AQYFVDKFKDHPFLDIQKEVGESSWFGFSFVIKEGAAIERKSLVNNLISAGIECRPIV
                     TGNFLKNERVLSYFDYSVHDTVANAEYIDKNGFFVGNHQIPLFNEIDYLRKVLK"
     gene            8646..9911
                     /gene="wzx"
     CDS             8646..9911
                     /gene="wzx"
                     /note="integral membrane protein; previously known as
                     orf8.9"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAC44886.1"
                     /db_xref="GI:1155022"
                     /translation="MVLTVKKILAFGYSKVLPPVIEQFVNPICIFIITPLILNHLGKQ
                     SYGNWILLITIVSFSQLICGGCSAWIAKIIAEQRILSDLSKKNALRQISYNFSIVIIA
                     FAVLISFLILSICFFDVARNNSSFLFAIIICGFFQEVDNLFSGALKGFEKFNVSCFFE
                     VITRVLWASIVIYGIYGNALLYFTCLAFTIKGMLKYILVCLNITGCFINPNFNRVGIV
                     NLLNESKWMFLQLTGGVSLSLFDRLVIPLILSVSKLASYVPCLQLAQLMFTLSASANQ
                     ILLPMFARMKASNTFPSNCFFKILLVSLISVLPCLALFFFGRDILSIWINPTFATENY
                     KLMQILAISYILLSMMTSFHFLLLGIGKSKLVANLNLVAGLALAASTLIAAHYGLYAI
                     SMVKIIYPAFQFYYLYVAFVYFNRAKNVY"
     gene            9901..10953
                     /gene="wzy"
     CDS             9901..10953
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAD46730.1"
                     /db_xref="GI:5678717"
                     /translation="MSIDLLFSITEIAIVFSCTIYIFTQCLLMRRIYLDKSILILLCL
                     LFFLVIIQLPELNVNGLVDSLKLSLPLLMVFIAFQKPKLCLWVIIALLFLNSAFNFLY
                     LKTFDKFSSFPFTFFILLFYLFRLGIGNLPVYKNKKFYALIFLFILIDIMQSLLINYR
                     GQILYSVICILILVFKVNLRKKIPYFFLMLPVLYVIIMAYIGFNYFNKGVTFFEPTAS
                     NIERTGMIYYLVSQLGDYIFHGMGTLNFLNNGGQYKTLYGLPSLIPNDPHDFLLRFFI
                     SIGVIGALVYHSIFFVFFRRISFLLYERNAPFIVVSCLLLLQVVLIYTLNPFDAFNRL
                     ICGLTVGVVYGFAKIR"
     gene            10931..11824
                     /gene="wbdL"
     CDS             10931..11824
                     /gene="wbdL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAD46731.1"
                     /db_xref="GI:5678718"
                     /translation="MDLQKLDKYTCNGNLDAPLVSIIIATYNSELDIAKCLQSVTNQS
                     YKNIEIIIMDGGSSDKTLDIAKSFKDDRIKIVSEKDRGIYDAWNKAVDLSIGDWVAFI
                     GSDDVYYHTDAIASLMKGVMVSNGAPVVYGRTAHEGPDRNISGFSGSEWYNLTGFKFN
                     YYKCNLPLPIMSAIYSRDFFRNERFDIKLKIVADADWFLRCFIKWSKEKSPYFINDTT
                     PIVRMGYGGVSTDISSQVKTTLESFIVRKKNNISCLNIQLILRYAKILVMVAIKNIFG
                     NNVYKLMHNGYHSLKKIKNKI"
     gene            11821..12945
                     /gene="wbdM"
     CDS             11821..12945
                     /gene="wbdM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAD46732.1"
                     /db_xref="GI:5678719"
                     /translation="MKIVYIITGLTCGGAEHLMTQLADQMFIRGHDVNIICLTGISEV
                     KPTQNINIHYVNMDKNFRSFFRALFQVKKIIVALKPDIIHSHMFHANIFSRFIRMLIP
                     AVPLICTAHNKNEGGNARMFCYRLSDFLASITTNVSKEAVQEFIARKATPKNKIVEIP
                     NFINTNKFDFDINVRKKTRDAFNLKDSTAVLLAVGRLVEAKDYPNLLNAINHLILSKT
                     SNCNDFILLIAGDGALRNKLLDLVCQLNLVDKVFFLGQRSDIKELMCAADLFVLSSEW
                     EGFGLVVAEAMACERPVVATDSGGVKEVVGPHNDVIPVSNHILLAEKIAETLKIDDNA
                     RKIIGMKNREYIVSNFSIKTIVSEWERLYFKYSKRNNIID"
     gene            13124..>14516
                     /gene="gnd"
     CDS             13124..>14516
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="AAD46733.1"
                     /db_xref="GI:5678720"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCITYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELATT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRIAASKVLSGPQAKLAGDKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENKGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHT"
BASE COUNT     4582 a   2221 c   2971 g   4742 t
ORIGIN
        1 gatctgatgg ccgtagggcg ctacgtgctt tctgctgata tctgggctga gttggaaaaa
       61 actgctccag gtgcctgggg acgtattcaa ctgactgatg ctattgcaga gttggctaaa
      121 aaacagtctg ttgatgccat gctgatgacc ggcgacagct acgactgcgg taagaagatg
      181 ggctatatgc aggcattcgt taagtatggg ctgcgcaacc ttaaagaagg ggcgaagttc
      241 cgtaagagca tcaagaagct actgagtgag tagagattta cacgtctttg tgacgataag
      301 ccagaaaaaa tagcggcagt taacatccag gcttctatgc tttaagcaat ggaatgttac
      361 tgccgttttt tatgaaaaat gaccaataat aacaagttaa cctaccaagt ttaatctgct
      421 ttttgttgga ttttttcttg tttctggtcg catttggtaa gacaattagc gtgagtttta
      481 gagagttttg cgggatctcg cggaactgct cacatctttg gcatttagtt agtgcactgg
      541 tagctgttaa gccaggggcg gtagcttgcc taattaattt ttaacgtata catttattct
      601 tgccgcttat agcaaataaa gtcaatcgga ttaaacttct tttccattag gtaaaagagt
      661 gtttgtagtc gctcagggaa attggttttg gtagtagtac ttttcaaatt atccattttc
      721 cgatttagat ggcagttgat gttactatgc tgcatacata tcaatgtata ttatttactt
      781 ttagaatgtg atatgaaaaa aatagtgatc ataggcaatg tagcgtcaat gatgttaagg
      841 ttcaggaaag aattaatcat gaatttagtg aggcaaggtg ataatgtata ttgtctagca
      901 aatgattttt ccactgaaga tcttaaagta ctttcgtcat ggggcgttaa gggggttaaa
      961 ttctctctta actcaaaggg tattaatcct tttaaggata taattgctgt ttatgaacta
     1021 aaaaaaattc ttaaggatat ttccccagat attgtatttt catattttgt aaagccagta
     1081 atatttggaa ctattgcttc aaagttgtca aaagtgccaa ggattgttgg aatgattgaa
     1141 ggtctaggta atgccttcac ttattataag ggaaagcaga ccacaaaaac taaaatgata
     1201 aagtggatac aaattctttt atataagtta gcattaccga tgcttgatga tttgattcta
     1261 ttaaatcatg atgataaaaa agatttaatc gatcagtata atattaaagc taaggtaaca
     1321 gtgttaggtg ggattggatt ggatcttaat gagttttcat ataaagagcc accgaaagag
     1381 aaaattacct ttatttttat agcaaggtta ttaagagaga aagggatatt tgagtttatt
     1441 gaagccgcaa agttcgttaa gacaacttat ccaagttctg aatttgtaat tttaggaggt
     1501 tttgagagta ataatccttt ctcattacaa aaaaatgaaa ttgaatcgct aagaaaagaa
     1561 catgatctta tttatcctgg tcatgtggaa aatgttcaag attggttaga gaaaagttct
     1621 gtttttgttt tacctacatc atatcgagaa ggcgtaccaa gggtgatcca agaagctatg
     1681 gctattggta gacctgtaat aacaactaat gtacctgggt gtagggatat aataaatgat
     1741 ggggtcaatg gctttttgat acctccattt gaaattaatt tactggcaga aaaaatgaaa
     1801 tattttattg agaataaaga taaagtactc gaaatggggc ttgctggaag gaagtttgca
     1861 gaaaaaaact ttgatgcttt tgaaaaaaat aatagactag catcaataat aaaatcaaat
     1921 aatgattttt gacttgagca gaaattattt atatttcaat ctgaaaaata aaggctgtta
     1981 ttatgaataa agtggcatta attactggta tcactgggca agatggctcc tatttggcag
     2041 aattattgtt agaaaaaggt tatgaagttc atggtattaa acgccgtgca tcttcattta
     2101 atactgagcg agtggatcac atctatcagg attcacattt agctaatcct aaactttttc
     2161 tacactatgg cgatttgaca gatacttcca atctgacccg tattttaaaa gaagttcaac
     2221 cagatgaagt ttacaatttg ggggcgatga gccatgtagc ggtatcattt gagtcaccag
     2281 aatacactgc tgatgttgat gcgataggaa cattgcgtct tcttgaagct atcaggatat
     2341 tggggctgga aaaaaagaca aaattttatc aggcttcaac ttcagagctt tatggtttgg
     2401 ttcaagaaat tccacaaaaa gagactacgc cattttatcc acgttcgcct tatgctgttg
     2461 caaaattata tgcctattgg atcactgtta attatcgtga gtcttatggt atgtttgcct
     2521 gcaatggtat tctctttaac cacgaatcac ctcgccgtgg cgagaccttt gttactcgta
     2581 aaataacacg cgggatagca aatattgctc aaggtcttga taaatgctta tacttgggaa
     2641 atatggattc tctgcgtgat tggggacatg ctaaggatta tgtcaaaatg caatggatga
     2701 tgctgcagca agaaactcca gaagattttg taattgctac aggaattcaa tattctgtcc
     2761 gtgagtttgt cacaatggcg gcagagcaag taggcataga gttagcattt gaaggtgagg
     2821 gagtaaatga aaaaggtgtt gttgtttcgg tcaatggcac tgatgctaaa gctgtaaacc
     2881 cgggcgatgt aattatatct gtagatccaa ggtattttag gcctgcagaa gttgaaacct
     2941 tgcttggcga tcctactaat gcgcataaaa aattaggatg gagccctgaa attacattgc
     3001 gtgaaatggt aaaagaaatg gtttccagcg atttagcaat agcgaaaaag aacgtcttgc
     3061 tgaaagctaa taacattgcc actaatattc cgcaagaata aaaaagataa tacattaaat
     3121 aattaaaaat ggtgctagat ttattagtac cattattttt ttttgggtga ctaatgttta
     3181 ttacatcaga taaatttaga gaaattatca agttagttcc attagtatca attgatctgc
     3241 taattgaaaa cgagaatggt gaatatttat ttggtcttag gaataatcga ccggccaaaa
     3301 attatttttt tgttccaggt ggtaggattc gcaaaaatga atctattaaa aatgctttta
     3361 aaagaatatc atctatggaa ttaggtaaag agtatggtat ttcaggaagt gtttttaatg
     3421 gtgtatggga acatttctat gatgatggtt ttttttctga aggcgaggca acacattata
     3481 tagtgctttg ttacacactg aaagttctta aaagtgaatt gaatctccca gatgatcaac
     3541 atcgtgaata cctttggcta actaaacacc aaataaatgc taaacaagat gttcataact
     3601 attcaaaaaa ttattttttg taatttttat taaaaattaa tatgcgagag aattgtatgt
     3661 ctcaatgtct ttaccctgta attattgccg gaggaaccgg aagccgtcta tggccgttgt
     3721 ctcgagtatt ataccctaaa caatttttaa atttagttgg ggattctaca atgttgcaaa
     3781 caacaattac gcgtttggat ggcatcgaat gcgaaaatcc aattgttatc tgcaatgaag
     3841 atcaccgatt tattgtagca gagcaattac gacagattgg taagctaacc aagaatatta
     3901 tacttgagcc gaaaggccgt aatactgcac ctgccatagc tttagctgct tttatcgctc
     3961 agaagaataa tcctaatgac gaccctttat tattagtact tgcggcagac cactctataa
     4021 ataatgaaaa agcatttcga gagtcaataa taaaagctat gccgtatgca acttctggga
     4081 agttagtaac atttggaatt attccggaca cggcaaatac tggttatgga tatattaaga
     4141 gaagttcttc agctgatcct aataaagaat tcccagcata taatgttgcg gagtttgtag
     4201 aaaaaccaga tgttaaaaca gcacaggaat atatttcgag tgggaattat tactggaata
     4261 gcggaatgtt tttatttcgc gccagtaaat atcttgatga actacggaaa tttagaccag
     4321 atatttatca tagctgtgaa tgtgcaaccg ctacagcaaa tatagatatg gactttgtcc
     4381 gaattaacga ggctgagttt attaattgtc ctgaagagtc tatcgattat gctgtgatgg
     4441 aaaaaacaaa agacgctgta gttcttccga tagatattgg ctggaatgac gtgggttctt
     4501 ggtcatcact ttgggatata agccaaaagg attgccatgg taatgtgtgc catggggatg
     4561 tgctcaatca tgatggagaa aatagtttta tttactctga gtcaagtctg gttgcgacag
     4621 tcggagtaag taatttagta attgtccaaa ccaaggatgc tgtactggtt gcggaccgtg
     4681 ataaagtcca aaatgttaaa aacatagttg acgatctaaa aaagagaaaa cgtgctgaat
     4741 actacatgca tcgtgcagtt tttcgccctt ggggtaaatt cgatgcaata gaccaaggcg
     4801 atagatatag agtaaaaaaa ataatagtta aaccaggaga agggttagat ttaaggatgc
     4861 atcatcatag ggcagagcat tggattgttg tatccggtac tgctaaagtt tcactaggta
     4921 gtgaagttaa actattagtt tctaatgagt ctatatatat ccctcaggga gcaaaatata
     4981 gtcttgagaa tccaggcgta atacctttgc atctaattga agtaagttct ggtgattacc
     5041 ttgaatcaga tgatatagtg cgttttactg acagatataa cagtaaacaa ttcctaaagc
     5101 gagattgata aatatgaata aaataacttg cttcaaagca tatgatatac gtgggcgtct
     5161 tggtgctgaa ttgaatgatg aaatagcata tagaattggt cgcgcttatg gtgagttttt
     5221 taaacctcaa actgtagttg tgggaggaga tgctcgctta acaagtgaga gtttaaagaa
     5281 atcactctca aatgggctat gtgatgcagg cgtaaatgtc ttagatcttg gaatgtgtgg
     5341 tactgaagag atatattttt ccacttggta tttaggaatt gatggtggaa tcgaggtaac
     5401 tgcaagccat aatccaattg attataatgg aatgaaatta gtaaccaaag gtgctcgacc
     5461 aatcagcagt gacacaggtc tcaaagatat acaacaatta gtagagagta ataattttga
     5521 agagctcaac ctagaaaaaa aagggaatat taccaaatat tccacccgag atgcctacat
     5581 aaatcatttg atgggctatg ctaatctgca aaaaataaaa aaaatcaaaa tagttgtgaa
     5641 ttctgggaat ggtgcagctg gtcctgttat tgatgctatt gaggaatgct ttttacggaa
     5701 caatattccg attcagtttg taaaaataaa taatacaccc gatggtaatt ttccacatgg
     5761 tatccctaat ccattactac ctgagtgcag agaagatacc agcagtgcgg ttataagaca
     5821 tagtgctgat tttggtattg catttgatgg tgattttgat aggtgttttt tctttgatga
     5881 aaatggacaa tttattgaag gatactacat tgttggttta ttagcggaag tttttttagg
     5941 gaaatatcca aacgcaaaaa tcattcatga tcctcgcctt atatggaata ctattgatat
     6001 cgtagaaagt catggtggta tacctataat gactaaaacc ggtcatgctt acattaagca
     6061 aagaatgcgt gaagaggatg ccgtatatgg cggcgaaatg agtgcgcatc attattttaa
     6121 agattttgca tactgcgata gtggaatgat tccttggatt ttaatttgtg aacttttgag
     6181 tctgacaaat aaaaaattag gtgaactggt ttgtggttgt ataaacgact ggccggcaag
     6241 tggagaaata aactgtacac tagacaatcc gcaaaatgaa atagataaat tatttaatcg
     6301 ttacaaagat agtgccttag ctgttgatta cactgatgga ttaactatgg agttctctga
     6361 ttggcgtttt aatgttagat gctcaaatac agaacctgta gtacgattga atgtagaatc
     6421 taggaataat gctattctta tgcaggaaaa aacagaagaa attctgaatt ttatatcaaa
     6481 ataaatttgc acctgagttc ataatgggaa caagaaatat atgaaagtac ttctgactgg
     6541 ctcaactggc atggttggta agaatatatt agagcatgat agtgcaagta aatataatat
     6601 acttactcca accagctctg atttgaattt attagataaa aatgaaatag aaaaattcat
     6661 gcttatcaac atgccagact gtattataca tgcagcggga ttagttggag gcattcatgc
     6721 aaatataagc aggccgtttg attttctgga aaaaaatttg cagatgggtt taaatttagt
     6781 ttccgtcgca aaaaaactag gtatcaagaa agtgcttaac ttgggtagtt catgcatgta
     6841 ccccaaaaac tttgaagagg ctattcctga gaaagctctg ttaactggtg agctagaaga
     6901 aactaatgag ggatatgcta ttgcgaaaat tgctgtagca aaagcatgcg aatatatatc
     6961 aagagaaaac tctaattatt tttataaaac aattatccca tgtaatttat atgggaaata
     7021 tgataaattt gatgataact cgtcacatat gattccggca gttataaaaa aaatccatca
     7081 tgcgaaaatt aataatgtcc cagagatcga aatttggggg gatggtaatt cgcgccgtga
     7141 gtttatgtat gcagaagatt tagctgatct tattttttat gttattccta aaatagaatt
     7201 catgcctaat atggtaaatg ctggtttagg ttacgattat tcaattaatg actattataa
     7261 gataattgca gaagaaattg gttatactgg gagtttttct catgatttaa caaaaccaac
     7321 aggaatgaaa cggaagctag tagatatttc attgcttaat aaaattggtt ggtcaagtca
     7381 ctttgaactc agagatggca tcagaaagac ctataattat tacttggaga atcaaaataa
     7441 atgattacat acccacttgc tagtaatact tgggatgaat atgagtatgc agcaatacag
     7501 tcagtaattg actcaaaaat gtttaccatg ggtaaaaagg ttgagttata tgagaaaaat
     7561 tttgctgatt tgtttggtag caaatatgcc gtaatggtta gctctggttc tacagctaat
     7621 ctgttaatga ttgctgccct tttcttcact aataaaccaa aacttaaaag aggtgatgaa
     7681 ataatagtac ctgcagtgtc atggtctacg acatattacc ctctgcaaca gtatggctta
     7741 aaggtgaagt ttgtcgatat caataaagaa actttaaata ttgatatcga tagtttgaaa
     7801 aatgctattt cagataaaac aaaagcaata ttgacagtaa atttattagg taatcctaat
     7861 gattttgcaa aaataaatga gataataaat aatagggata ttatcttact agaagataac
     7921 tgtgagtcga tgggcgcggt ctttcaaaat aagcaggcag gcacattcgg agttatgggt
     7981 acctttagtt ctttttactc tcatcatata gctacaatgg aagggggctg cgtagttact
     8041 gatgatgaag agctgtatca tgtattgttg tgccttcgag ctcatggttg gacaagaaat
     8101 ttaccaaaag agaatatggt tacaggcact aagagtgatg atattttcga agagtcgttt
     8161 aagtttgttt taccaggata caatgttcgc ccacttgaaa tgagtggtgc tattgggata
     8221 gagcaactta aaaagttacc aggttttata tccaccagac gttccaatgc acaatatttt
     8281 gtagataaat ttaaagatca tccattcctt gatatacaaa aagaagttgg tgaaagtagc
     8341 tggtttggtt tttccttcgt tataaaggag ggagctgcta ttgagaggaa gagtttagta
     8401 aataatctga tctcagcagg cattgaatgc cgaccaattg ttactgggaa ttttctcaaa
     8461 aatgaacgtg ttttgagtta ttttgattac tctgtacatg atacggtagc aaatgccgaa
     8521 tatatagata agaatggttt ttttgtcgga aaccaccaga tacctttgtt taatgaaata
     8581 gattatctac gaaaagtatt aaaataacta acgaggcact ctatttcgaa tagagtgcct
     8641 ttaagatggt attaacagtg aaaaaaattt tagcgtttgg ctattctaaa gtactaccac
     8701 cggttattga acagtttgtc aatccaattt gcatcttcat tatcacacca ctaatactca
     8761 accacctggg taagcaaagc tatggtaatt ggattttatt aattactatt gtatcttttt
     8821 ctcagttaat atgtggagga tgttccgcat ggattgcaaa aatcattgca gaacagagaa
     8881 ttcttagtga tttatcaaaa aaaaatgctt tacgtcaaat ttcctataat ttttcaattg
     8941 ttattatcgc atttgcggta ttgatttctt ttcttatatt aagtatttgt ttcttcgatg
     9001 ttgcgaggaa taattcttca ttcttattcg cgattattat ttgtggtttt tttcaggaag
     9061 ttgataattt atttagtggt gcgctaaaag gttttgaaaa atttaatgta tcatgttttt
     9121 ttgaagtaat tacaagagtg ctctgggctt ctatagtaat atatggcatt tacggaaatg
     9181 cactcttata ttttacatgt ttagccttta ccattaaagg tatgctaaaa tatattcttg
     9241 tatgtctgaa tattaccggt tgtttcatca atcctaattt taatagagtt gggattgtta
     9301 atttgttaaa tgagtcaaaa tggatgtttc ttcaattaac tggtggcgtc tcacttagtt
     9361 tgtttgatag gctcgtaata ccattgattt tatctgtcag taaactggct tcttatgtcc
     9421 cttgccttca actagctcaa ttgatgttca ctctttctgc gtctgcaaat caaatattac
     9481 taccaatgtt tgctagaatg aaagcatcta acacatttcc ctctaattgt ttttttaaaa
     9541 ttctgcttgt atcactaatt tctgttttgc cttgtcttgc gttattcttt tttggtcgtg
     9601 atatattatc aatatggata aaccctacat ttgcaactga aaattataaa ttaatgcaaa
     9661 ttttagctat aagttacatt ttattgtcaa tgatgacatc ttttcatttc ttgttattag
     9721 gaattggtaa atctaagctt gttgcaaatt taaatctggt tgcagggctc gcacttgctg
     9781 cttcaacgtt aatcgcagct cattatggcc tttatgcaat atctatggta aaaataatat
     9841 atccggcttt tcaattttat tacctttatg tagcttttgt ctattttaat agagcgaaaa
     9901 atgtctattg atttactttt ttcaattact gaaatcgcaa ttgttttttc ttgcactatt
     9961 tacatattta ctcaatgttt gttaatgcgg aggatctatt tagataaaag tattttaatt
    10021 cttttatgct tgctcttttt tttagtaatc attcaacttc ctgagcttaa tgtaaacggt
    10081 ttggtcgatt ctttaaagtt atcactgcct ttattgatgg tctttatcgc ttttcaaaaa
    10141 ccgaaattat gcttgtgggt tattattgca ttgttgtttt tgaactctgc atttaatttt
    10201 ttatatttaa agacattcga taagtttagc tcatttcctt ttactttttt tatattgctg
    10261 ttttacttgt ttagattggg aattggtaat ttaccggttt ataaaaataa aaaattttac
    10321 gcgttgattt ttctctttat attaatagac ataatgcagt cattgttaat aaattatagg
    10381 gggcagattt tatattccgt aatttgcatc ctgatacttg tgtttaaagt taatttaaga
    10441 aaaaagattc catacttttt tttaatgctg ccagttttat atgtaattat tatggcttat
    10501 attggtttta attatttcaa taaaggcgta actttttttg aacctacagc aagtaatatt
    10561 gaacgtacgg ggatgatata ttatttggtt tcacagcttg gtgattatat attccatggt
    10621 atggggacat taaatttctt aaataacggc ggacaatata agacgttata tggacttcca
    10681 tcattaattc ctaatgaccc tcatgatttt ttattacggt tctttataag tattggtgtg
    10741 ataggagcat tggtttatca ttctatattt tttgtttttt ttaggagaat atctttctta
    10801 ttatatgaga gaaatgctcc tttcattgtt gtaagttgtt tgttactgtt acaagttgtg
    10861 ttaatttata cattaaaccc ttttgatgct tttaatcgat tgatttgcgg gcttacagtt
    10921 ggagttgttt atggatttgc aaaaattaga taagtatacc tgtaatggaa atttagacgc
    10981 tccacttgtt tcaataatca ttgcaactta taattctgaa cttgatatag ctaagtgttt
    11041 gcaatcggta actaatcaat cttataagaa tattgaaatc ataataatgg atggaggatc
    11101 ttctgataaa acgcttgata ttgcaaaatc gtttaaagac gaccgaataa aaatagtttc
    11161 agagaaagat cgtggaattt atgatgcctg gaataaagca gttgatttat ccattggtga
    11221 ttgggtagca tttattggtt cagatgatgt ttactatcat acagatgcaa ttgcttcatt
    11281 gatgaagggg gttatggtat ctaatggcgc ccctgtggtt tatgggagga cagcgcacga
    11341 aggtcccgat aggaacatat ctggattttc aggcagtgaa tggtacaacc taacaggatt
    11401 taagtttaat tattacaaat gtaatttacc attgcccatt atgagcgcaa tatattctcg
    11461 tgatttcttc agaaacgaac gttttgatat taaattaaaa attgttgctg acgctgattg
    11521 gtttctgaga tgtttcatca aatggagtaa agagaagtca ccttatttta ttaatgacac
    11581 gacccctatt gttagaatgg gatatggtgg ggtttcgact gatatttctt ctcaagttaa
    11641 aactacgcta gaaagtttca ttgtacgcaa aaagaataat atatcctgtt taaacataca
    11701 gctgattctt agatatgcta aaattctggt gatggtagcg atcaaaaata tttttggcaa
    11761 taatgtttat aaattaatgc ataacgggta tcattcccta aagaaaatca agaataaaat
    11821 atgaagattg tttatataat aaccgggctt acttgtggtg gagccgaaca ccttatgacg
    11881 cagttagcag accaaatgtt tatacgcggg catgatgtta atattatttg tctaactggt
    11941 atatctgagg taaagccaac acaaaatatt aatattcatt atgttaatat ggataaaaat
    12001 tttagaagct tttttagagc tttatttcaa gtaaaaaaaa taattgtcgc cttaaagcca
    12061 gatataatac atagtcatat gtttcatgct aatattttta gtcgttttat taggatgctg
    12121 attccagcgg tgcccctgat atgtaccgca cacaacaaaa atgaaggtgg caatgcaagg
    12181 atgttttgtt atcgactgag tgatttttta gcttctatta ctacaaatgt aagtaaagag
    12241 gctgttcaag agtttatagc aagaaaggct acacctaaaa ataaaatagt agagattccg
    12301 aattttatta atacaaataa atttgatttt gatattaatg tcagaaagaa aacgcgagat
    12361 gcttttaatt tgaaagacag tacagcagta ctgctcgcag taggaagact tgttgaagca
    12421 aaagactatc cgaacttatt aaatgcaata aatcatttga ttctttcaaa aacatcaaat
    12481 tgtaatgatt ttattttgct tattgctggc gatggcgcat taagaaataa attattggat
    12541 ttggtttgtc aattgaatct tgtggataaa gttttcttct tggggcaaag aagtgatatt
    12601 aaagaattaa tgtgtgctgc agatcttttt gttttgagtt ctgagtggga aggttttggt
    12661 ctcgttgttg cagaagctat ggcgtgtgaa cgtcccgttg ttgctaccga ttctggtgga
    12721 gttaaagaag tcgttggacc tcataatgat gttatccctg tcagtaatca tattctgttg
    12781 gcagagaaaa tcgctgagac acttaaaata gatgataacg caagaaaaat aataggtatg
    12841 aaaaatagag aatatattgt ttccaatttt tcaattaaaa cgatagtgag tgagtgggag
    12901 cgcttatatt ttaaatattc caagcgtaat aatataattg attgaaaata taagtttgta
    12961 ctctggatgc aatagtttct ctatgctgtt tttttactgg ctccgtattt ttacttatag
    13021 ctggattttg ttatatatca gtattaatct gtctcaactt catctagact acattcaagc
    13081 cgcgcatgcg tcgcgcggtg actacacctg acaggagtat gtaatgtcca agcaacagat
    13141 cggcgtcgtc ggtatggcag tgatggggcg caacctggcg ctcaacatcg aaagccgcgg
    13201 ttataccgtc tccatcttca accgctcccg cgagaaaact gaagaagttg ttgccgagaa
    13261 cccggataag aaactggttc cttattacac ggtgaaagag ttcgtcgagt ctcttgaaac
    13321 cccacgtcgt atcctgttaa tggtaaaagc aggggcggga actgatgctg ctatcgattc
    13381 cctgaagccg tatctggata aaggcgacat cattattgat ggtggcaaca ccttcttcca
    13441 ggacactatc cgtcgtaacc gtgaactgtc cgcggaaggc tttaacttca tcggtaccgg
    13501 cgtgtccggc ggtgaagagg gcgccctgaa aggcccatct atcatgccag gtggccagaa
    13561 agaagcgtat gagctggttg cgcctatcct gaccaagatt gctgcggttg ctgaagatgg
    13621 cgaaccatgt ataacttaca tcggtgctga cggtgcgggt cactacgtga agatggtgca
    13681 caacggtatc gaatatggcg atatgcagct gattgctgaa gcctattctc tgcttaaagg
    13741 cggccttaat ctgtctaacg aagagctggc aaccactttt accgagtgga atgaaggcga
    13801 gctaagtagc tacctgattg acatcaccaa agacatcttc accaaaaaag atgaagaggg
    13861 taaatacctg gttgatgtga tcctggacga agctgcgaac aaaggcaccg gtaaatggac
    13921 cagccagagc tctctggatc tgggtgaacc gctgtcgctg atcaccgaat ccgtattcgc
    13981 tcgctacatc tcttctctga aagaccagcg cattgcggca tctaaagtgc tgtctggtcc
    14041 gcaggctaaa ctggctggtg ataaagcaga gttcgttgag aaagtccgtc gcgcgctgta
    14101 cctgggtaaa atcgtctctt atgcccaagg cttctctcaa ctgcgtgccg cgtctgacga
    14161 atacaactgg gatctgaact acggcgaaat cgcgaagatc ttccgcgcgg gctgcatcat
    14221 tcgtgcgcag ttcctgcaga aaattactga cgcgtatgct gaaaacaaag gcattgctaa
    14281 cctgttgctg gctccgtact tcaaaaatat cgctgatgaa tatcagcaag cgctgcgtga
    14341 tgtagtggct tatgctgtgc agaacggtat tccggtaccg accttctctg cagcggtagc
    14401 ctactacgac agctaccgtt ctgcggtact gccggctaat ctgattcagg cacagcgtga
    14461 ttacttcggt gcgcacacgt ataaacgcac tgataaagaa ggtgtgttcc acaccg
//
