LOCUS       EU296413               10812 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O112ab O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296413
VERSION     EU296413.1  GI:187880573
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O112ab"
     gene            1041..2432
                     /gene="wzx"
     CDS             1041..2432
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37085.1"
                     /db_xref="GI:187880574"
                     /translation="MNLLKGTIIYTLSNLTIKFGAVLLLPILTHLLNPEEYGMVGLYV
                     TLTSFLTIILGLGFYTPLMKTQSEQKSNIATSSHVNFCAIIFLIAVYLLLLLFLYFIL
                     KSSLVIDLLGEVKLDSKLIYLAVIVSMFSAINIIMNTSFRMDENYILVAILSILSFVL
                     FYSSAIIFIKQFNYGGLGYIYGNLLSSIIIFFISFTCYYRKLSIAFSWHNVKFLCGNG
                     IPMVFVELSDKIIEASDRFILVRYISLSSLGVYTLALTGCKVLNVVFNSYISAILPSI
                     YKSVESREDSNYIKVKLENAYVLVVMMVFLGQLISKEIIDIIFPASYSNLFFVFILAL
                     PAISLQYYYFLDFYFHRSEDSRFILCFTIATSIINIILNLIFVPKYGVYASLLSTYIS
                     YLIRTLVEIKFIARRYKLKFSVLKVLMGSFVLYTVPLLYHYGYYTVLLRVTLSIVLFI
                     GIIMFYKKSKIFN"
     misc_feature    1041..9416
                     /note="O-antigen gene cluster"
     gene            2452..3630
                     /gene="wzy"
     CDS             2452..3630
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37086.1"
                     /db_xref="GI:187880575"
                     /translation="MVNAKNFVLIIFDVICLFLNVFVYVPRLIIGVLTVDKTLIITFP
                     LMLSVLAYNFIPPETYDLYRHYENYQNFLNNNEVSFIRDFFLYFLFTVGRLFDLNNGF
                     IAFVSCYILYFYWLKILYINFDYKANKKNFPLYFFIFFITPPLVIYTGVRFSTGLILT
                     LYGIVNWIQLRNVKLGIVYIICGLTAHFSMALIALIFIFTSYFNNIFSNKYLRVGLVF
                     SALLVISFNEQLVRFLTTVIEFINQLFDWNFISIDTYITGKWGLERAQELNSTGQLVL
                     GIRNYGMLFLILLFCPLISFNKNKYTNFYYVMCAVTILVFPFTAVFDRYGTFLIFFIV
                     YYLSTLQKKTNIEIIYFLMLIVYLIFFRTTDIKDNISVYTESYSNILNLSLIKVLLGI
                     "
     gene            3630..4610
                     /gene="wfeF"
     CDS             3630..4610
                     /gene="wfeF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeF"
                     /protein_id="ACD37087.1"
                     /db_xref="GI:187880576"
                     /translation="MRKYENNIVISVIIPTYNNDKTIIEAIQSIVNVEMPVKYEVLVI
                     DDGSVDQTSNLIAEYISQRKDNTIKYFYQKNSGVSAARNVGIKNALGKYVLFLDADDT
                     YSEHVLKEIDEILSTNNDIYVFSYEKLNNKKAIISNKKNIITHYNNKYDFLNAYISGE
                     LFGCVSVCSCIFSKESLISNNIFYDSKIAYGEDQWFLINALKVCNDVEFRLELNLLNY
                     TYNTESATNRFNLRRFDAITMLERINQEAFKVNQDIINARINKELVNIATIYTKFNSL
                     QKSIMFVNNVILPKIKTDSDKRLYMFKDMVFTRLPCLYIILYKLYIQLFK"
     gene            4617..5798
                     /gene="wfeG"
     CDS             4617..5798
                     /gene="wfeG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeG"
                     /protein_id="ACD37088.1"
                     /db_xref="GI:187880577"
                     /translation="MNILIIIPKLCNGGIEKIASNFSQYLPERYNQFVYSIMSQDSSY
                     DFAVKPIILNKSLGKTFIGKLITFFYRLHLARKIIRENNIDICISFGERCNIINILSM
                     GKTKKIITIHSQLSIENKTKGLYGKVTTLFSKLLYKNADATVAVSEIVKKDACGLLNL
                     DANNVEIIYNGHDIGYIKDKSTDYKEFDTPVIDFVSVGRITYAKGHYHLLRSPAIVKE
                     TYPNVILYIVGTYEKDNLKSIIDHLIEKYDLYDNVIFTGFSDNPYPYIKSAKALILSS
                     IFEGFPGVVIESIALGTPVIATDCGGASEVLRSPDAKIKNNTGDVEITKLGVLLPKFD
                     GIYDINLDFSPTEKKMAQVMNDIISGKLKFNEDFLITKANEYDIDSMMKKYTDLIEKI
                     K"
     gene            5798..6769
                     /gene="gae"
     CDS             5798..6769
                     /gene="gae"
                     /note="C5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gae"
                     /protein_id="ACD37089.1"
                     /db_xref="GI:187880578"
                     /translation="MKEATTSYKSIHTILEYFGWSHEDYWHINYKVNYDFSKFNYYWD
                     LTKKYNWFSGPFDQNNIPLYLGTDSKLHYSPIFLGHYALGAYQEYLLSNDKKAYDDFI
                     RIADWLVENTTTYKKCSGIWINTYPMSTFNLKGKWQSCLAQAKGISVLCRAYYLTQNL
                     KYLKVALDAFESFKIYKEEGGVKVRHKNVFFWEEYPTITNSIVLNGHIFAVWALYDLS
                     FVLKDKNEFKQQWADIYSYYKTSLIILKENYKLWDTHYWTRYDIWDKHFNVSSLFYHN
                     LHIKQFYILAVLGDDDDFKKIANKWAGKLKNPLFRMYALIKKVVFRL"
     gene            6766..7647
                     /gene="wfeH"
     CDS             6766..7647
                     /gene="wfeH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeH"
                     /protein_id="ACD37090.1"
                     /db_xref="GI:187880579"
                     /translation="MIMNVIDKIIMGLKSIYLSFLYTIYNLFLNENRFEETNEVHVVV
                     SLTCFPGRIKKVYLTLESIMAQQYNNFKVVLYLSHEEFPKGLEDLPRSLIRLHKRGVD
                     INFTCENIRSYKKLHYALSDFPELPVITADDDVLYPSRWVNDFMESHKLFHDDILFAR
                     GHQITFDRNGNVKKYISFGKPAGYSASSLYIPTGVSGILYPPGCFFQDVQNKDIFMKL
                     APNADDIWYKVMTLLNGRKSRLIYKKPIHYPPILGTQKISLRKQNLSKQHLNNDTQLL
                     NLIDYYSIELADFKKKE"
     gene            7653..8393
                     /gene="wfeI"
     CDS             7653..8393
                     /gene="wfeI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeI"
                     /protein_id="ACD37091.1"
                     /db_xref="GI:187880580"
                     /translation="MELVSIIMPCFNNEATIKESINSVLSQSYKYWELIIINDNSTDD
                     SQNIINDYVNSDERIKLITNEKNIGAGGSRNKGIEIANGRFIAFLDADDVWLPEKLAK
                     QIKFMRDGNYALTYSYYQKFDSTGLGAIVRSPATTTYRKLLYSNVIGCLTGMYDTDFL
                     GKCYMPLIRKRQDMGLWLSILKKCNKAYCLPEVLAYYRIDSGMTKNKLDAAKYQWRFY
                     RDVTKLNLIQSSWYFVWYSVLGLVKYKK"
     gene            8406..9416
                     /gene="gne"
     CDS             8406..9416
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACD37092.1"
                     /db_xref="GI:187880581"
                     /translation="MTILVTGGAGYIGSHTVIELQKNGFDVVIVDNFCNSSPSVLKRI
                     ELITGKEIKCYDIDLLDFNSLQKVFVTNNINSVIHFASLKSVSESLIKPTEYYHNNLS
                     GCLNVLKIMKEHNVNNFIFSSSATVYGSNNNNPVSESSSVGTATNPYGKSKIFLEQIL
                     SDCCKSNPNLNVTCLRYFNPVGAHSSGLIGESPNGVPANLVPYLTQVALGKLPVLHIY
                     GNDYDTKDGTGVRDFIHVTDLANGHIAALKKINGHNGFKVYNLGTGRGYSVLEVVRCF
                     EAITGKSIPIEFSPRREGDIAESWANVSAANYELEWFAKKTLTDMLRDAWKWQTLNPN
                     GL"
BASE COUNT     3567 a   1591 c   2046 g   3608 t
ORIGIN
        1 ctcctggtaa ctcatgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgaa
       61 ttagaatctc tccttgaaca gcgcgtgaag cgtcaactgc tggcggaagt acagtctatc
      121 tgtccgccgg gcgtgaccat tatgaacgtg cgtcagggcg aacctttagg tttgggccac
      181 tccattttgt gtgcacgacc cgccattggt gacaacccat ttgtcgtggt actgccggac
      241 gttgtgatcg acgacgccag tgccgacccg ctgcgctaca accttgctgc catgattgcg
      301 cgtttcaatg aaacgggccg tagccaggtg ctggcaaaac gtatgccggg tgacctttct
      361 gaatactccg ttattcagac caaagagccg ctggatcgtg aaggcaaagt cagccgcatt
      421 gttgaattta tcgaaaaacc ggatcagccg cagacgctgg actcagacat catggccgtt
      481 ggtcgctatg tgctttctgc cgatatttgg ccggaacttg aacgcactca acctggtgca
      541 tgggggcgta ttcagctgac tgatgccatt gctgaactgg cgaaaaaaca gtccgttgat
      601 gcaatgctga tgactggtga cagctacgac tgcggtaaaa aaatgggcta tatgcaggcg
      661 tttgtgaagt atggcttacg caacctgaaa gaaggggcga agttccgtaa aggtattgag
      721 aaactgttaa gcgaataatg aaaatctgac cggatgtaac ggttgataag aaaattataa
      781 cggcggtgaa gattcctgtg gagagtaatt tgttgcgatt accactgtcg ttattcaact
      841 ataagcataa aaaagttaaa tataatttgg ggattttaag tatatggaaa cgcctttgtc
      901 tactgaataa gtaaggtatt aactgtaaga aaagcgtgaa tggtgttccc tacataattt
      961 aaagatgttc agtctactgg tagctgttaa gccaggggcg gtagcgtgga taattttgaa
     1021 aaaaaagatt agagcataaa atgaatttac ttaaagggac aataatatat acattgtcaa
     1081 atttgacaat taaatttggt gcagttttac tgcttcctat attaactcat ttacttaatc
     1141 cagaagagta tgggatggta ggattgtatg ttacacttac ttcctttctt acaataattt
     1201 tagggcttgg tttttataca cccttaatga aaactcagtc ggagcagaaa agcaatattg
     1261 caacttctag ccatgtgaat ttttgtgcaa taatatttct tatcgcagtt tatttattat
     1321 tgcttttatt tctttacttt atattgaagt cgtctttagt tattgatttg ttgggagagg
     1381 tgaaactaga tagcaaatta atttatttgg cagttattgt atcaatgttt tctgcaatta
     1441 atattattat gaacacgtcg tttagaatgg acgagaatta tatattagtt gcaatattgt
     1501 caattttatc ttttgtgtta ttttattcct ctgctataat attcattaaa cagttcaatt
     1561 atggaggcct tggctatata tatggcaatt tattaagttc aataatcatt ttttttataa
     1621 gtttcacttg ttactataga aaattatcta ttgcattttc atggcacaac gtgaaatttt
     1681 tgtgtgggaa tggaattcca atggtttttg tagagttatc ggataaaata attgaagcca
     1741 gcgatagatt tattttagtc agatatattt cactatcctc tttaggcgta tatacactag
     1801 cactaacagg atgtaaagta ttaaacgtag ttttcaattc ttatattagc gctatccttc
     1861 cctctatcta taaaagcgtt gaaagccgtg aagattcaaa ttatataaaa gttaagcttg
     1921 agaatgcata cgtgcttgta gtaatgatgg tttttttggg acagttgata tcaaaagaaa
     1981 taattgacat aatttttcct gcatcatatt caaatttatt ttttgtattt atattagctt
     2041 tgcctgcaat atctctacaa tattattatt ttttagattt ctattttcat agaagtgaag
     2101 atagccgatt tattttatgt tttacaatag caacctcgat aataaatatt attctaaact
     2161 taatttttgt tcccaaatat ggagtttatg catctctttt atcaacgtat atatcctatt
     2221 taatcagaac gctagtagaa ataaaattta tagcgagacg gtataaattg aagtttagtg
     2281 tgcttaaagt ccttatggga agctttgttc tgtatactgt tcctttatta tatcattatg
     2341 gttattatac agtgttgttg agagtaactt tgtcaattgt tttatttata ggtattatta
     2401 tgttttataa aaaaagtaag atattcaatt aaaagtgagt gctaagtgat catggttaat
     2461 gctaaaaatt ttgtattaat tatttttgat gtaatatgtc tttttcttaa tgtttttgtt
     2521 tatgtcccac gtctaattat tggtgtttta acagtagata aaacattgat tattacattt
     2581 cccttgatgt tatcagtgtt ggcatataat tttattccac cagaaacata tgatctttac
     2641 agacattatg aaaactatca aaattttttg aataataatg aagtaagttt tatcagggat
     2701 ttttttcttt attttttatt taccgtaggg aggctttttg atttaaataa tggatttata
     2761 gcatttgtat cttgttatat cctctatttt tattggttaa aaatattata cattaacttt
     2821 gattataaag ccaacaagaa aaatttcccg ttatactttt ttatattttt tattactcca
     2881 cctcttgtta tatatacagg cgttcgattt tccacagggc ttatattaac actatatggg
     2941 attgtaaact ggattcaatt acggaatgta aaacttggta tagtatatat tatttgcggg
     3001 ttaacagccc atttttcaat ggcattgatt gctttaatat ttattttcac atcatatttc
     3061 aataatatct tttcaaataa atatttaagg gttgggctcg tattttctgc attacttgta
     3121 atctcattta atgaacagtt agtaaggttt ttgacaacag taattgagtt cattaatcag
     3181 ttatttgatt ggaactttat tagtatcgat acatatatta ctggtaaatg ggggctggag
     3241 agggcgcagg aattgaattc aaccggacaa ttagtattag gtatacgtaa ttatggtatg
     3301 ctctttctaa tattgctatt ttgcccacta atatctttta ataaaaataa atatacgaac
     3361 ttttattatg taatgtgtgc tgttacaata ttagttttcc cttttacagc agtatttgat
     3421 agatatggaa catttttaat tttttttatt gtatactatc ttagtactct tcagaaaaaa
     3481 actaacattg aaataatata ttttttaatg ttgattgtat atttgatttt ctttagaaca
     3541 acagacatca aagataatat ttccgtatac actgaaagct atagtaatat attgaatctt
     3601 tctttaatta aagtactgtt aggaatataa tgcgtaaata tgaaaataac attgttataa
     3661 gtgtgattat tccaacatat aacaatgata aaacaatcat agaggctatt caatctatag
     3721 taaatgttga gatgcctgtg aaatatgagg tactagtaat agacgatggt tcggtagatc
     3781 agacatcaaa tttaattgca gaatatataa gtcaaagaaa agataatact attaaatatt
     3841 tttatcaaaa aaacagtggt gtaagcgctg cgagaaacgt gggaataaaa aatgcattag
     3901 ggaaatatgt tctattttta gacgcagatg atacatatag tgaacatgtg ttaaaagaaa
     3961 tagatgaaat attaagtact aacaatgata tatatgtgtt ttcatatgaa aaactgaata
     4021 ataaaaaggc tataatatca aataaaaaaa atattataac tcattataat aataaatatg
     4081 attttttaaa cgcctatatc tcaggtgaac ttttcgggtg cgttagtgtt tgctcttgca
     4141 tttttagtaa agaatcatta attagcaata atatttttta cgatagtaaa attgcttatg
     4201 gggaagatca atggtttttg ataaatgctc tcaaggtttg caatgatgta gagttcagat
     4261 tagaactaaa tttactgaat tatacttata atacggagtc ggcgactaat cgtttcaacc
     4321 taagacgttt tgacgcgatt acaatgctag aaagaatcaa tcaagaagca tttaaggtta
     4381 atcaagatat aattaatgca agaattaata aagaattggt aaatatagca acaatatata
     4441 cgaaattcaa ttcgttacag aaatcaataa tgtttgttaa taacgttata ctcccaaaaa
     4501 taaaaacgga tagtgacaaa agactatata tgttcaaaga tatggttttc actagattac
     4561 catgtttata tataatactt tataaactct atattcagct atttaagtaa actaatatga
     4621 atattcttat aattattcct aagttatgta acggtgggat tgaaaagatt gcatcaaact
     4681 tttctcaata cttaccagaa agatataatc aatttgtata ttcaatcatg tcacaagact
     4741 cttcttatga ttttgcggta aagcccatta tattaaataa aagtttaggt aaaaccttta
     4801 ttggaaaatt gattacattt ttctacaggc ttcatttggc acgtaaaatt attagagaaa
     4861 ataacataga tatatgcatt agttttgggg agagatgtaa tattataaat attctatcta
     4921 tggggaaaac aaaaaaaatc attactatac atagtcagct atctattgaa aataaaacga
     4981 aaggattata tggaaaagta acaacactat tttcaaaatt gctttataaa aatgcggatg
     5041 ctactgtagc cgtatcagaa atagtgaaga aggatgcatg cggtttatta aatttagatg
     5101 cgaataatgt agagataatt tataatggac acgatattgg ttatatcaaa gataagtcta
     5161 ctgattataa agaatttgat acacctgtga tcgacttcgt gtctgtaggg cgaattacat
     5221 atgctaaagg gcattatcat ctattgagaa gtcctgccat agtaaaggag acgtatccta
     5281 atgtaatact atatattgtt ggcacgtatg aaaaagataa tttaaaaagc ataattgatc
     5341 atttaattga gaaatacgat ttatatgata atgtgatttt taccggcttt agtgacaatc
     5401 catatcctta cataaaatca gcaaaggctc ttatacttag ttcaattttt gaaggttttc
     5461 caggcgttgt tatcgagagt attgcgcttg gaactcctgt catagcgaca gattgtggcg
     5521 gtgcttcaga agttttaaga agtccagatg ccaaaataaa gaataatacg ggagatgtgg
     5581 aaattactaa attaggagta ctgttaccaa aattcgatgg gatttatgat attaatttgg
     5641 atttttcgcc cacagaaaaa aagatggcgc aagttatgaa tgatattata tcagggaagc
     5701 tgaaatttaa tgaagacttt ttaattacaa aagcaaacga atatgatata gattctatga
     5761 tgaaaaaata tacggactta atagagaaaa taaaataatg aaagaagcta ctacctcata
     5821 taaatcaatt catactatat tagaatattt tggttggtct catgaagatt attggcatat
     5881 caactataaa gtaaattatg atttttcaaa atttaattat tattgggatt tgacaaaaaa
     5941 gtataattgg tttagtggac cttttgatca aaataatatt cctttatatc ttggcactga
     6001 ttcaaaactg cattactcac caattttttt agggcattat gctcttggag catatcaaga
     6061 atatttatta tcaaatgata aaaaagcata tgacgatttt ataagaatag cggattggct
     6121 ggttgaaaat acaacgacat ataaaaaatg ttctggaata tggataaata catacccaat
     6181 gtcaactttt aacttaaaag gtaaatggca atcgtgttta gcccaggcaa aaggtatatc
     6241 tgtcctatgt agagcttatt atcttacgca aaatttaaaa tatttgaagg tagctcttga
     6301 cgcatttgaa tctttcaaaa tatataaaga agaaggtgga gttaaggtaa ggcataaaaa
     6361 cgtattcttt tgggaggaat atccaactat aaccaattct atcgtattga atggacatat
     6421 ttttgcagtt tgggcattgt acgatttatc ttttgtatta aaagataaga acgagtttaa
     6481 gcaacaatgg gcggatattt acagttatta taaaacatca ctgattatat taaaagagaa
     6541 ttataagtta tgggatacgc actattggac tagatatgat atatgggata aacattttaa
     6601 tgtttcaagt ttgttttatc ataatctcca tattaaacaa ttctatatat tggctgtttt
     6661 gggagatgat gatgatttca agaaaattgc taataaatgg gctggtaaat taaaaaatcc
     6721 gcttttcaga atgtatgcgc ttataaagaa agtagttttt aggttatgat tatgaatgtt
     6781 attgataaaa ttattatggg tttgaaaagt atatatttaa gctttcttta tacaatatat
     6841 aatttatttc ttaatgaaaa tagattcgaa gagacaaatg aagtacatgt tgttgtaagt
     6901 ttgacgtgtt ttcctggacg tataaaaaaa gtatatctta ctttagagtc aattatggct
     6961 caacaatata ataactttaa agtggtattg tacttgtctc atgaagagtt tccaaagggt
     7021 ttagaggatt tacctcgtag tttaatacga ttacataaaa gaggtgtcga tattaatttt
     7081 acgtgtgaaa atattcgatc ttataagaag ttacattatg cgctgagtga tttcccagaa
     7141 ctacctgtaa taacagctga tgatgatgtc ttatatccct cgcgatgggt aaatgatttt
     7201 atggagtcac ataagctttt tcatgatgat atattatttg cgagggggca tcagataaca
     7261 tttgaccgaa atggaaatgt taaaaaatat atctctttcg gaaaaccagc tggatattca
     7321 gcatcaagtc tatatattcc tacaggagta agtggaattc tatatccgcc aggatgtttt
     7381 tttcaagatg tgcagaataa agatattttt atgaagttgg cacctaacgc agatgatata
     7441 tggtataaag taatgacttt gttaaacggt cgaaaatcca gattgatcta taaaaagcca
     7501 attcattatc cacctatcct tggcacgcag aagatctcct tgcgtaaaca aaacttatcg
     7561 aaacaacatt taaataatga tacacaatta ttaaatttaa ttgattatta ttctatcgaa
     7621 ttagctgatt ttaagaaaaa ggaatagctc ctatggaatt agtttcaatt ataatgcctt
     7681 gttttaataa tgaagcaact ataaaagaaa gtattaatag cgtactatct caaagttata
     7741 aatactggga gttaattatt atcaatgaca actcaacaga tgattcacaa aatattatta
     7801 acgactatgt gaatagtgat gaaagaataa aactgattac taatgaaaaa aatatcggag
     7861 ctggaggtag tagaaataaa ggaattgaaa ttgcaaatgg aagattcatt gcttttttag
     7921 atgcagatga tgtttggttg ccagaaaaac ttgcaaaaca aataaagttt atgagagatg
     7981 gtaattatgc actgacatat agttattatc aaaagtttga ttcaactgga ttgggagcga
     8041 tcgtccggtc acctgcgaca acaacttata ggaaattgtt atacagtaat gttattggat
     8101 gtctaacagg aatgtatgat actgattttt tgggtaaatg ctatatgcct ttaattcgta
     8161 aacgacaaga tatggggctt tggttgtcaa tattgaaaaa gtgtaataaa gcatattgct
     8221 tacctgaagt tttggcatat tatagaattg actcaggaat gacaaaaaat aaacttgatg
     8281 cggcaaaata tcagtggcga ttttataggg atgtaactaa acttaatttg attcaatcaa
     8341 gttggtattt tgtttggtat tcagttttag gtttggtaaa gtacaaaaaa taataaggag
     8401 gagtgatgac aattttagtc actggtggtg ctggatatat tggcagtcac actgttattg
     8461 aattacagaa aaatggtttt gacgtggtaa tagtagataa tttttgtaat tcatcgccat
     8521 cggttttaaa aagaattgaa ttaattacag gaaaggaaat taaatgttac gatattgatc
     8581 ttctggattt taattctttg cagaaggttt ttgtaactaa taatattaac tctgtgattc
     8641 attttgcatc tttaaaatca gtttctgaat cactaatcaa accaactgaa tattatcata
     8701 ataatttgag tggttgcctc aatgtcttga aaataatgaa agaacataac gtgaataatt
     8761 tcattttcag ttcttcggca actgtatatg gcagtaataa caacaatcct gtttcagaat
     8821 cttcatcagt cggcaccgca acaaatcctt atggaaaatc caagattttt ttagagcaaa
     8881 tattgtctga ttgctgtaaa tcaaatccta atttaaatgt aacttgcctg aggtatttca
     8941 atccggttgg tgcacattct tcaggtttga taggagaatc tccgaatggt gttcctgcca
     9001 atttagttcc atatttaact caggttgctt taggtaaatt gcccgtatta catatatatg
     9061 gtaatgacta tgataccaag gatgggacgg gagtaagaga ttttattcat gttacggatt
     9121 tagctaatgg acacatagct gcgctgaaaa aaataaatgg tcataatggt tttaaagttt
     9181 ataatcttgg tactgggcga ggttatagtg tgttagaagt cgttagatgt tttgaggcta
     9241 ttacaggaaa aagtattcca atcgaatttt cgcctcgcag agaaggtgat atcgctgaaa
     9301 gttgggcaaa tgtttccgca gcaaattatg agttagagtg gttcgctaaa aagacattaa
     9361 ctgatatgtt acgagatgca tggaaatggc aaacgttaaa tccaaatgga ttataaagat
     9421 atttaattta tattaaacct tgcttattta agataatgta aaccgcacct ttgcggtaac
     9481 cacccctgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg
     9541 atggggcgca accttgcgct caacatcgaa agccgtggtt ataccgtctc tattttcaac
     9601 cgttcccgtg aaaagaccga agaagtgatt gccgaaaatc caggcaagaa actggttcct
     9661 tactatacgg tgaaagagtt tgttgaatct ttggaaacgc ctcgtcgcat cctgttaatg
     9721 gtgaaagcag gtgcaggcac ggatgctgct attgattccc ttaagccata tctcgataaa
     9781 ggcgacatca tcattgatgg cggtaacacc ttcttccagg acaccattcg tcgtaaccgt
     9841 gagctttctg ccgaaggctt taacttcatt ggtaccggtg tctccggtgg tgaagaaggc
     9901 gcgctgaaag gtccttccat tatgcctggt gggcagaaag aagcctatga actggttgct
     9961 ccgatcctga ccaaaatcgc tgcagtggct gaagacggcg agccatgcgt tacctatatt
    10021 ggtgccgatg gtgcaggtca ctatgtgaag atggttcaca acggtattga atacggcgat
    10081 atgcagctga ttgccgaagc ctattctctg ctaaaaggtg gcctgaacct taccaacgaa
    10141 gaattggcac agacctttac cgaatggaat aacggtgaac tgagcagcta cctgatcgac
    10201 attaccaaag acatcttcac taaaaaagat gaagacggta actacctggt tgatgtgatc
    10261 ctggatgaag cggcaaacaa aggtacgggc aaatggacca gccagagcgc actggatctc
    10321 ggcgaaccgc tgtcgctgat taccgagtct gtgtttgcac gttatatctc ttctctgaaa
    10381 gatcagcgcg ttgccgcttc taaagttctc tctggcccgc aagcgcagcc agcaggcgac
    10441 aaggctgagt tcatcgaaaa agttcgtcgt gcgctgtatc tgggcaaaat cgtttcttac
    10501 gctcagggct tctctcagtt gcgtgctgcg tctgaagaat acaactggga tctgaactac
    10561 ggcgaaatcg cgaagatttt ccgtgctggc tgcattatcc gtgcgcagtt cctgcagaaa
    10621 atcaccgatg cctatgccga aaatccgcag atcgctaacc tgctgctggc tccgtacttc
    10681 aagcaaattg ccgatgacta tcagcaggcg ctgcgtgatg tcgttgctta tgcagtacag
    10741 aacggtatcc cggttccgac cttcgccgct gcggttgcct attatgacag ctaccgtgcc
    10801 gctgttctgc ct
//
