LOCUS       AY573377               13272 bp    DNA     linear   BCT 30-JAN-2006
DEFINITION  Escherichia coli serotype O114 O-antigen gene cluster, complete
            sequence.
ACCESSION   AY573377
VERSION     AY573377.1  GI:50429169
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Feng,L., Wang,W., Tao,J., Guo,H., Krause,G., Beutin,L. and Wang,L.
  TITLE     Identification of Escherichia coli O114 O-antigen gene cluster and
            development of an O114 serogroup-specific PCR assay
  JOURNAL   J. Clin. Microbiol. 42 (8), 3799-3804 (2004)
   PUBMED   15297533
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Feng,L., Wang,W., Tao,J., Guo,H., Krause,G., Beutin,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2004) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P. R. China
COMMENT     E. coli O114 O-antigen gene cluster.
            Name: E. coli O114 O-antigen gene cluster.
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O114"
                     /db_xref="taxon:562"
     gene            1138..2214
                     /gene="rmlB"
     CDS             1138..2214
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="AAT77169.1"
                     /db_xref="GI:50429170"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSGSERYVFEHADICDAAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDKIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     FNNNDWCRRVQDGSYQRERLGLVK"
     gene            2211..3089
                     /gene="rmlA"
     CDS             2211..3089
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="AAT77170.1"
                     /db_xref="GI:50429171"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGDGCQFGINITYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLVLGDNIFFGQGFTPKIRDAAKRTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKSIKPSKRGELEITAVNEVYLKKNILTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLSNEKLYELSQNLIKN
                     QYGIYLLKLLENKYID"
     gene            3094..3486
                     /gene="fdtA"
     CDS             3094..3486
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA"
                     /protein_id="AAT77171.1"
                     /db_xref="GI:50429172"
                     /translation="MKNLNLKVFGDERGSLISFESNKNIPFEIKRVYYIYATREKISR
                     GFHAHKKLKQVVVAVHGSCDFCVDNGLVRNTYHLNSPSQALLIDSLCWREMHNFSEDC
                     VLMVLASDYYDPDDYIHDYNSFLRYLNA"
     gene            3479..3925
                     /gene="fdtC"
     CDS             3479..3925
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtC"
                     /protein_id="AAT77172.1"
                     /db_xref="GI:50429173"
                     /translation="MLKLVEYDRTFLDLSMKWLSDPEIASLVMVPSFTKVQQECFFNS
                     LPTRENYYIKGLIYNGYPIGAAGIKNIYKEKGEYWGYIGEKQFWGKGIGKDIIRLIIN
                     NAKILGLTSLYLNVHRDNERAQKLYEKYGFIRMSVRGSVILMERSI"
     gene            3925..4923
                     /gene="wbuL"
     CDS             3925..4923
                     /gene="wbuL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAT77173.1"
                     /db_xref="GI:50429174"
                     /translation="MNIVLQNIEDKIIQHGYYISLSQDSLINNLFSRNDFLKYQSKKQ
                     NKNYIIFTLINKQSDEISIQLVTCYDLKEKGQIFFSPITGAFSDIYMPLKKIDVQVLT
                     DFLQYIEVYIQIHFSAKKIIYKLPPELYNCEDNNCLLNAFHNRSWEIHNIDLNFHLEL
                     ISLDEFRRGLSSGTRQRLNKISRGDVEFNKVNDEANRFLVYETIRLNRKAQGYPMTMS
                     WDNLNDLYLSLSEAVSFFTLKYNESIVASAICLELSPENIYVFYWGENPEFRKLSPIV
                     KLAEQLYSFYLQAGCRTLDIGISTENSKPNQGLIDFKKNIGCSLTKKITVEKNLGR"
     gene            4925..6031
                     /gene="fdtB"
     CDS             4925..6031
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtB"
                     /protein_id="AAT77174.1"
                     /db_xref="GI:50429175"
                     /translation="MNIEFLNLKKINQRFADEIRFSFERVLESGRYIQGNELKMFEEE
                     FASFCGVKHCIGVANGLDALVLVLKAWKLMNKCKDGDEVIVPSNTFIASILAITESGL
                     KPVLVEPDPITNNITLSSIRSAITSKTKVLLPVHLYGSICNMSEIMDIAKENDSLVLE
                     DSAQAHGAVHKGKKAGAWGDAAAFSFYPGKNLGALGDAGAVTTNDSDLAMIIRSLGNY
                     GSSKKYNHIYKGVNSRLDELQAAILRVKLRYLETDIMQRRAIARDYCNNIKNKYVLLP
                     CSSVPEEHVWHLFVIKTDRRVELKSYLAANGIQTLEHYPVPPHKQLAYKEWSSKSYPI
                     SEKLHQSVLSLPLDPTMSEEEVRYVINIINGFKG"
     gene            6028..7281
                     /gene="wzx"
     CDS             6028..7281
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAT77175.1"
                     /db_xref="GI:50429176"
                     /translation="MKKLLTVTFFTGMLTFFKMISGFIISKIVAIYTGPSGIVLFGQI
                     QSIVSALNGVINSPVGPGIVRFTAENHRKGLEHCYKWWIAGLSWVCIISAIIIPLSIT
                     LSSHIAFWTLGSEKYSWVIILTACLLPLTAIGTFISSVINGFQNYKVYVLLGIISAFL
                     SLIIMIALIVFGKINGALLAISVQNALIGGVMIIYASSQPWFNLRWLYGRVHKTYYKD
                     IQRYIVMAITSAIVVPGSLIIVRNIIIHYVGWTEAGYWQAVWKISETYLAIITIALST
                     YYLPRLSSLSQKDDIIKEISSSLKIIIPLVMFFSMGIYLSRDFIISLLFTKEFLGARM
                     LFQVQLIGDILKIASWLYAYVMISKGMTQLFVISEVLFSLLFISFSLVFIRCYGLIGV
                     TYAYCLNYFIYLVFSVVVVKRAKYD"
     gene            7274..8143
                     /gene="wbuM"
     CDS             7274..8143
                     /gene="wbuM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAT77176.1"
                     /db_xref="GI:50429177"
                     /translation="MIKNHVSVFIVSYNHKNFIDCCIKSVIAQLSASYDLTVIDNCSV
                     DGTVDELKRLNKIYKFSLIMNDQNYGLPSTLNKALSLASGEYICFLAADDYMMFDRLT
                     TQISFLNQNQQYYACAGTQLKVDDSNVLCDLSKQKNIISSFTVIDKNNIFKSTNIIYS
                     PTSIYRTEVLKKIGGYKEDILIEDLYIYYKAASLGYKMALLPNLFCFYRIHKGNSHTK
                     LKWMFENKLKILAEFSEQSFYRDLSDLIHLEGFYSLSQQHKKDAIKILPRVLHRLDSK
                     YLYAGLFNLLLKW"
     gene            8137..9468
                     /gene="wzy"
     CDS             8137..9468
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAT77177.1"
                     /db_xref="GI:50429178"
                     /translation="MVTLFMEIILFISLSLFLILVYRVIANFSGVLALDFISFWFYTY
                     IVFAFLGSLIIITGFGDDSYFVQPLSAHARNVFFGGSLMVLYAGLGTFSIFLLVLSLF
                     LGKHRYKEKLIEKCTSESYDKITLAMLFIILMLVFFYYLISTFPSPLYLGLINASSED
                     IALRRIEVTKNLSNIANTYIISIGMLMSYIVSYAYLALFSIHKKYPIRTFLSVLIASI
                     FILSTGEKGPFLFYILGALICCSYAQGKIRRVSIKLIAITLFFLFLIYFIFVSSELSM
                     ITALIFDRIFIAQVISVYLSFDFYSNLGDIGFASLSNVFTKLYDSKSVMPASEELMSV
                     YYPEMLKLGGWNVNGLFISEAWANFGVLGAIISPVIVGIENGILLSILMKIKKSPMQC
                     AFYAYFTVKCVYFLTSFNAYLYHSDWIVCLLVLVLICFLNAIGKHFILKYK"
     gene            9396..10172
                     /gene="wbuN"
     CDS             9396..10172
                     /gene="wbuN"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuN"
                     /protein_id="AAT77178.1"
                     /db_xref="GI:50429179"
                     /translation="MLVSACADLFSQRYRKAFYSKIQIILWEGGVKNTIAAFDLCDTL
                     VKGNTTFIFLDSYFKNNKKYTLYRKVSRLLPFRVLLKFLFLFRLDLNRKLALRFLKGQ
                     NKLALQKHAKYLVDNVFIFNDDIVTLIKKCVDCKLKVYIVSASLDIIVQEVADKLSID
                     YVSSILSFKNDMCSGNLEKDILFIKKNIFLPNCSDYIDIDIKNLFYISDNIQDIELIK
                     KSFIGYGYYNKKNHFLFVKEGIKRFSLGEALADLNENNCK"
     gene            10251..11030
                     /gene="wbuO"
     CDS             10251..11030
                     /gene="wbuO"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuO"
                     /protein_id="AAT77179.1"
                     /db_xref="GI:50429180"
                     /translation="MFMFYLPFVYYYKTRLRYLHKLLSWMLIYLFPLFFSCYLSSNYS
                     ISILSFCLFIILVQSLYEVGYIQNDCETIKKEDSPTLRLTSVEFQYYENNKFLIYSSR
                     LVFSFILSVLLSYFVSSLVWAIWFILPIFYVYNNVRNRFNLILHFLLVFCRYCLPVLC
                     LTGNYQVTLMMIFVFPLLNLIERMSEKKFKFQFISRFLASKLTLFRALYYLFFLSLFY
                     LISINCRGDANIQNILYLICYYSVYRFSIYFFSVLFYKINR"
     gene            11039..11851
                     /gene="wbuP"
     CDS             11039..11851
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAT77180.1"
                     /db_xref="GI:50429181"
                     /translation="MRCKHAVIMCIYNGDNVNNVKLAVDSILNQTVNNHLYIYVDGEV
                     NPFLNTYLNKEESNHQIFIFRSEFNLGLAYGLNFLIDKIQSKSYEYISRMDGDDISLL
                     DRLERQELYLNKHPDVDVIGSYCTEFGSDCSLPVKKLPQYHDELVRFSVLRCPLIHPT
                     VMFRAKVFKHGLRYPINTYLSEDLALWYEMIYLGLKFGNVPDVLLKYRLTDSTISRRK
                     GLKKAGNEIALRFSYMKKMKLITLSNLILLSFKFISHFMPLIITKIMYKKLR"
BASE COUNT     4062 a   2054 c   2663 g   4491 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcgntc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtgca atctatctgc ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccggacgtt gtgatcgacg acgccagcgc cgacccgcta
      301 cgttacaacc ttgctgccat gattgcacgc ttcaacgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggcga tctctctgaa tactctgtca tccagaccaa agagccgctg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggcca
      541 gaacttgaac gcactcagcc tggtgcatgg ggacgtattc agctgactga tgccattgct
      601 gaactggcga aaaaacagtc cgttgatgct atgctgatga caggtgacag ctacgactgt
      661 ggtaaaaaaa tgggctatat gcaggcgttc gtgaagtatg gattacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaaacg aataatgaaa atctgaccga
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatatgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgccacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactaag agccgctaat ttaacagcat gctctgaagt aatatggaat aaattaagtg
     1141 aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata
     1201 aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcacttgctg atgtttctgg ttctgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gctgctgcaa tggcgcggat ttttgctctg catcagccgg atgcagtgat gcacctggct
     1381 gctgaaagcc atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt
     1441 gttggtactt atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac
     1501 aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtttatgg cgatctgcct
     1561 catcctgacg aagtaaataa taaagaagaa ttacccttat ttactgagac aacagcttac
     1621 gcaccaagca gcccttattc tgcatcaaaa gcgtccagcg atcatttagt ccgcgcgtgg
     1681 aaacgtacct atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat
     1741 catttcccgg aaaaattgat tccattggtt attctcaatg ctctggaagg taaagcatta
     1801 cctatttatg gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac
     1921 aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga taagattgtc
     1981 ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgtcc gggacacgat
     2041 cgccgttatg cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa
     2101 acgtttgaga gcgggattcg taaaacaatt aattggtatt ttaataataa tgattggtgc
     2161 cgacgagttc aggatggctc ttatcaacgt gagcgtttgg ggttagttaa atgaaaggaa
     2221 ttattttagc cggtggttct ggaacaaggc tttatccaat tacaatgggg gtttcaaagc
     2281 agctattgcc catttatgat aaaccgatga tttattatcc attatcggtt ttaatgctag
     2341 caggtataca agatattctt attattacaa ctccagaaga tcaacaaggg ttcataagat
     2401 tacttgggga tggatgccaa tttggtataa atattacata tgcaattcag ccaagtccag
     2461 atggtttagc tcaagcattt attattggtg aaaactttat tggaaatgat acagtatgcc
     2521 ttgtgcttgg agataacatc ttttttggac aaggttttac tcccaaaatc cgggatgctg
     2581 caaaaagaac atcaggtgcc acagtctttg gatatcaagt aatggatcct gagagatttg
     2641 gagtggtgga gtttgataga aattttaaag cgttaagtat tgaagaaaaa cctgctaacc
     2701 caaaatcaaa ttgggctgtc acaggattat atttttatga taataatgtc attgatattg
     2761 ctaagagtat taaaccctcc aaacgtggtg aattggaaat tactgcagtt aatgaagttt
     2821 atctcaaaaa aaatatttta actgttgaat tactaggccg aggttttgca tggcttgata
     2881 caggaactca tgacagtttg attgaagctg gcagttttgt agaaacagtt caaaaaaggc
     2941 aagggatgat ggtagcctgt cctgaggaga tagcttggag gaatggctgg ttaagtaacg
     3001 aaaaacttta tgagcttagc caaaatttaa ttaagaacca atatggcatt tatcttctta
     3061 aactattaga aaataaatat atagattaat aatatgaaaa atctaaacct taaagtgttt
     3121 ggtgatgaac gcggttcatt gatttcgttt gagtcaaata aaaatatacc ttttgagatt
     3181 aaaagagttt attatatata cgccacaagg gaaaaaataa gtagagggtt tcatgcccat
     3241 aaaaaattaa agcaagttgt tgttgctgtt cacggttctt gcgatttctg tgtcgacaac
     3301 ggactggttc gaaacactta tcatctaaac tcaccatctc aagcattatt gattgattct
     3361 ttatgctgga gagagatgca taatttctct gaagactgtg tcctgatggt attggctagt
     3421 gattattatg atcctgatga ttatattcac gattataata gttttttgag gtatttaaat
     3481 gcttaaatta gttgagtatg atcggacctt tcttgattta agtatgaaat ggttgtcgga
     3541 tccagaaata gcaagtttag ttatggtgcc ttctttcact aaagtacaac aggagtgttt
     3601 ttttaattct cttccaacaa gagaaaatta ttatatcaag ggattaatat acaatggata
     3661 tcctattggg gctgcaggta taaaaaatat atataaagaa aaaggtgagt actggggata
     3721 tataggtgag aagcaatttt ggggaaaagg tataggaaaa gatattattc ggctcataat
     3781 taataatgca aaaatacttg ggcttacatc tttgtattta aatgttcata gagataatga
     3841 gcgcgcacaa aaactatatg aaaaatatgg gtttattagg atgtctgttc gcggcagtgt
     3901 aattttgatg gagcggagta tttagtgaat atcgtattac aaaatataga agacaaaatt
     3961 atccaacatg gatattatat ttctttatcc caggatagtt tgataaataa tctattttcg
     4021 aggaatgact ttttaaagta tcaaagtaaa aaacagaaca aaaattatat tatatttaca
     4081 ttaattaata aacagtctga tgagattagc atacagcttg taacttgcta tgacttgaag
     4141 gaaaagggac agatattttt ttctccaatt acgggagcat tttcagatat atatatgccg
     4201 ctaaaaaaaa tagatgtgca agttttaacg gattttttac aatatattga ggtttatatt
     4261 caaatacact ttagcgcaaa aaaaataata tataaacttc ctcctgaatt atataactgt
     4321 gaagataata actgtctgtt aaatgctttt cataataggt catgggaaat tcataacatt
     4381 gatcttaatt ttcatttaga gttgataagt ctagatgaat ttagacgtgg tttaagtagc
     4441 ggtacacgtc aaagattaaa taaaataagt cgtggagatg tagaatttaa taaagttaat
     4501 gatgaagcta atagattttt ggtatatgaa acgattagat taaatagaaa agcgcagggt
     4561 tatcctatga ctatgtcttg ggataatctt aatgatcttt atttatcctt atctgaggct
     4621 gtaagcttct ttacgctgaa atataatgaa tctatagttg catctgcaat atgtttagag
     4681 ttgagtccag aaaatatata tgttttttat tggggggaaa atcctgagtt caggaaatta
     4741 tcccctattg taaaattagc cgaacaatta tattcttttt atttgcaagc aggatgtcgc
     4801 actttagata ttggtatatc gacggaaaac tctaaaccta atcaagggtt aattgatttt
     4861 aagaaaaata ttgggtgtag tctaactaag aaaatcacag ttgagaaaaa tttagggagg
     4921 taatatgaat atcgaattct taaatttaaa aaaaataaac cagcgatttg ccgatgagat
     4981 tcgtttttca tttgagcgag ttttagaatc tgggagatat attcaaggta atgaacttaa
     5041 gatgtttgag gaagagtttg cttctttttg tggcgttaag cattgtatcg gggtcgcaaa
     5101 tggccttgat gcgttagttt tggttttaaa agcatggaag ttaatgaata aatgtaagga
     5161 cggtgatgag gttatcgttc cctcgaatac ttttattgca tcaatactgg ctataacaga
     5221 atctgggctc aaaccagtat tggtggaacc tgatcccata acaaataata taacgttatc
     5281 ttctataagg tctgcaatta catctaaaac aaaggtttta ctacctgtcc atctctatgg
     5341 aagcatatgt aatatgtctg aaataatgga tattgcaaaa gaaaatgatt cgcttgtact
     5401 tgaagacagc gctcaggcac atggcgcagt acataaaggt aagaaggctg gagcttgggg
     5461 ggatgctgct gctttcagtt tttatcccgg gaaaaaccta ggggccttag gagatgctgg
     5521 ggctgtaact acgaatgata gtgatttagc catgattatt cgttctcttg gcaattatgg
     5581 ctcaagcaaa aagtataacc atatctataa aggagttaac agccgtcttg atgaactaca
     5641 ggcggcaatt ttaagggtta aattaaggta tttagaaaca gacattatgc aaaggcgtgc
     5701 aattgctcgc gattattgta ataatattaa aaataaatat gtattgctac cctgctcatc
     5761 cgtcccggaa gagcatgttt ggcatctatt tgtaataaaa acggacagaa gggttgaact
     5821 gaaaagctat cttgccgcta atggtataca aacattagaa cattatcctg tgccaccaca
     5881 taagcaattg gcatataaag agtggtcatc caaatcatac cctatatcgg aaaaacttca
     5941 tcagagtgtg ttatcattac cacttgatcc tacaatgagt gaagaagagg ttcgatatgt
     6001 aattaatata ataaatggtt ttaaaggatg aaaaaattat taacagtaac cttttttacc
     6061 gggatgttaa cttttttcaa gatgatttcc ggttttataa tatctaaaat tgttgctatt
     6121 tacactggac cttcagggat tgttctgttt ggtcaaattc agagtattgt atcggcatta
     6181 aatggtgtta ttaactcccc ggttggtccg gggattgttc gttttactgc agaaaatcat
     6241 aggaaaggat tagaacattg ctacaagtgg tggattgcag gtttaagttg ggtatgtata
     6301 atatcagcaa ttatcatccc tcttagtatt acactttcat ctcatattgc tttttggacg
     6361 ctggggtcgg aaaaatattc atgggttata attttaacag catgtttatt accactaacg
     6421 gcaataggga cattcatatc gtcagtaata aacggatttc agaattataa agtatatgtt
     6481 ttattaggta taatctcagc ttttttatcg ttgattataa tgattgccct aatagtattt
     6541 ggtaaaatta atggtgctct gctagcaata tcagtacaaa atgctttaat aggaggggtg
     6601 atgataatct atgcatcatc acaaccgtgg tttaatttgc gatggttgta tgggagagtt
     6661 cataaaactt attacaaaga tattcaacgc tatatcgtca tggcgataac aagtgctatt
     6721 gttgttcctg gctcattaat tatagtccgc aatataataa ttcattatgt aggctggaca
     6781 gaagctggtt attggcaagc tgtttggaaa atatctgaaa cctatcttgc tattattaca
     6841 atcgcattaa gtacgtatta tttacccaga ctttcttctt tatcacaaaa agatgatata
     6901 ataaaagaaa tatcttcttc attaaagata ataatcccat tggtgatgtt tttctcaatg
     6961 ggtatttatt tatctagaga ttttattatt tctttattat ttactaaaga gtttttgggt
     7021 gcacgtatgt tgtttcaagt gcaactaatt ggtgatattc ttaagattgc aagttggtta
     7081 tatgcatatg taatgatttc aaaagggatg actcagttat ttgttatttc tgaagtgtta
     7141 ttttctcttt tatttatatc tttttctctc gtctttatta ggtgttatgg cctgattgga
     7201 gttacttacg catattgttt gaattatttt atttatttgg ttttttctgt tgttgttgta
     7261 aaaagggcta agtatgatta agaatcatgt ttcagttttc atagtgtctt ataatcataa
     7321 aaatttcata gattgttgca taaagtccgt tattgcacaa ttatctgctt catacgattt
     7381 aactgtaata gacaattgct ctgttgatgg aaccgttgat gagttaaaac ggctaaataa
     7441 aatatataaa ttctcactta taatgaatga ccaaaattat ggtctcccat ccactttaaa
     7501 taaagcatta tctttagcat ctggggagta catatgcttt ttagctgctg atgattatat
     7561 gatgtttgat agattaacca cgcagatatc ttttttgaat cagaatcaac aatattatgc
     7621 ttgtgcgggg actcaactca aggtggatga ttctaatgtg ttatgcgatt tgagcaaaca
     7681 aaaaaacata atatcatcat ttaccgtaat tgataaaaat aatatattta aatctacaaa
     7741 tataatatac tcccctacat cgatttatag aaccgaagta ttaaaaaaga taggagggta
     7801 taaggaggat attttaatag aagatctata tatatattat aaagctgcat ctttaggcta
     7861 taaaatggct cttcttccaa atttgttttg tttttatcga atacataagg gtaacagcca
     7921 tacaaaatta aagtggatgt ttgaaaataa gttaaaaatc ctagcagagt tctcagagca
     7981 atcattttat agagatttgt ctgatttaat tcatttagaa ggcttttact ctttatctca
     8041 acaacataaa aaagatgcaa taaaaatact tcccagggtt ttacataggc tcgatagtaa
     8101 atacttgtac gctggtttat ttaatttgct tcttaaatgg tgactctatt tatggagatt
     8161 atattattta tttcattgtc gcttttttta atattggttt atagggttat agcaaacttt
     8221 tctggtgtgc ttgctttaga ttttatttct ttttggtttt atacttatat cgtatttgct
     8281 tttttgggtt ctctaataat tataacaggg tttggtgatg atagttattt tgtgcaaccg
     8341 ctatctgcac atgccaggaa tgtttttttt ggcggttcgt tgatggtttt atatgctggg
     8401 ttaggtacgt tttcaatttt tttattagta ttgtcattat ttttaggtaa gcataggtat
     8461 aaagaaaaat taattgaaaa atgtactagt gagtcttatg acaagataac attagcaatg
     8521 ctttttatta tattgatgct tgtatttttt tactatctta ttagcacttt cccaagccca
     8581 ttatatttgg gtttgataaa tgcatcatca gaagatattg cattaaggcg cattgaagtt
     8641 actaaaaatc ttagcaatat tgctaataca tatattatct caataggaat gttaatgtcg
     8701 tatatcgttt cttatgcata cttggcactt ttttcaattc ataagaaata tcctattcgt
     8761 acattccttt ctgtattaat tgctagtata ttcatattat caacaggtga gaaaggacct
     8821 tttttattct atatattagg agccttaata tgttgttcat atgctcaggg gaaaatcaga
     8881 agagtatcta ttaaattgat agccataacg cttttttttc tttttttaat ttattttatc
     8941 tttgtgtcga gtgaattatc tatgattact gctcttatct tcgataggat ttttatagct
     9001 caggtaatat cagtttattt atcatttgat ttttattcaa acttaggaga tattggtttt
     9061 gcttcgctgt caaatgtatt tactaagtta tatgactcta agagtgtgat gccagcatca
     9121 gaagagctaa tgtcagttta ttatcctgag atgcttaaat taggtggatg gaatgttaat
     9181 gggttattta tttcagaagc atgggcaaac tttggtgtcc tgggggcaat tatttcacct
     9241 gtaattgtag gtatagaaaa tggtattttg ctaagtattt tgatgaagat aaaaaagagt
     9301 ccaatgcagt gtgcttttta tgcctatttt acagttaagt gtgtttattt tttaacatcc
     9361 tttaatgcat atctatatca ttcagattgg attgtatgct tgttagtgct tgtgctgatt
     9421 tgttttctca acgctatagg aaagcatttt attctaaaat acaaataata ttatgggagg
     9481 gtggggtgaa aaatacaatt gctgcatttg atttatgcga tacccttgtc aaagggaata
     9541 ctacatttat ttttttggat tcatatttta aaaataacaa gaaatatacc ttatatagaa
     9601 aagtaagtag actcttacca tttcgtgttt tattaaagtt tttgttttta tttcgccttg
     9661 atttaaatag aaaattagca ttgcgtttcc ttaaagggca aaataaactt gctttacaaa
     9721 aacatgcaaa atatttagtg gataatgtct tcatttttaa tgacgacatt gttacattaa
     9781 taaaaaaatg tgtcgattgt aaattaaaag tttatatagt ttctgcctct cttgacatta
     9841 ttgtccagga agtagctgat aaactatcaa ttgattacgt ttcaagtata ttatccttta
     9901 agaatgatat gtgctctggt aatctggaaa aagatatttt gtttattaaa aagaatattt
     9961 ttcttccaaa ttgttctgac tacattgata tcgatattaa aaatctgttt tatattagcg
    10021 ataatatcca ggatattgag ttaataaaaa aatcttttat cgggtatggt tactataaca
    10081 aaaaaaacca tttcttgttc gttaaagaag gtattaaaag attttcatta ggcgaagcac
    10141 ttgccgattt aaatgaaaat aactgtaaat gaattcgggg atgggtgttt ttcagatgtt
    10201 ttattatggt atgtggtgtt tacttataac aatttaagaa gaggtgcttg atgtttatgt
    10261 tttatcttcc atttgtttat tattataaaa caagattgag atatttacac aaacttttat
    10321 cgtggatgtt aatctatctc tttccattat ttttttcttg ctatctatca agtaattata
    10381 gtataagtat tctttccttt tgtcttttta ttattctggt tcaaagttta tatgaagtag
    10441 gttatattca aaatgattgc gagacgataa aaaaagaaga tagtccaact cttaggttaa
    10501 catctgtaga gtttcaatat tacgaaaata ataaattttt aatctactct tcccgattgg
    10561 tattttcttt tatattgagt gttttattaa gttattttgt cagttctctt gtgtgggcta
    10621 tctggtttat attacctata ttttatgttt ataataatgt aagaaataga tttaatttga
    10681 ttttgcattt cttattggtt ttttgtagat attgtttgcc tgttttatgt ttgacaggga
    10741 attatcaggt cacgcttatg atgatttttg tttttcctct attaaattta atagaaagga
    10801 tgtcggagaa aaaattcaaa tttcaattta ttagtcgttt tttagcatca aaattaacac
    10861 tatttcgcgc tctttattat ttattctttt tatccctgtt ttatctgata tcgataaatt
    10921 gtaggggtga tgccaatata caaaatatct tatacctaat atgttattat tctgtttatc
    10981 ggttttcaat atattttttt tcagttcttt tttacaagat taataggtag cttttatcat
    11041 gcgctgtaaa catgctgtca ttatgtgcat ttataatggt gacaatgtta ataatgttaa
    11101 actagcagtt gatagtattc ttaatcagac cgttaataac catttatata tttatgttga
    11161 tggggaggtt aatccatttc taaatacgta tttaaataaa gaagagtcta atcatcaaat
    11221 ttttattttt cgatctgagt ttaatctggg attggcatat gggttgaatt ttttaattga
    11281 taaaatccaa agcaagagct atgaatatat atcaaggatg gatggcgatg atatttcatt
    11341 gttagaccgg ctggaacgac aggagctata tttaaacaaa catcctgatg ttgatgtaat
    11401 cggttcatat tgtactgaat ttgggagtga ttgttcgcta cctgtaaaaa aactacctca
    11461 atatcatgat gaattggtta ggtttagtgt cttacgttgt cctttaattc atcctacagt
    11521 aatgtttaga gcgaaagtgt ttaaacatgg tcttcgatat ccaattaata catatttatc
    11581 agaggactta gcattatggt atgagatgat atatttaggg ctgaagtttg ggaatgttcc
    11641 agatgtttta cttaaatata ggttgacgga tagcaccata agtagacgta aaggtttaaa
    11701 aaaagctggg aatgagatag cattacgatt ctcctacatg aaaaagatga aactaataac
    11761 tttgagtaat ttaatattgt tgtcattcaa atttatatcc cactttatgc cgcttattat
    11821 cacaaaaata atgtacaaga aacttagata aaatattatg agttatattg tgttaaattt
    11881 tatatgaaac cttttactgc cgctgcatgc ggtaacccca gtcaggagta aacaatgtca
    11941 aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gttcaacatc
    12001 gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgaaaagac ggaagaagtg
    12061 attgccgaaa atccaggcaa gaaactggtt ccttactata cggtgaaaga gtttgttgaa
    12121 tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct
    12181 gctattgatt ccctcaaacc atacctcgat aaaggtgaca tcatcattga tggtggtaat
    12241 accttcttcc aggacaccat tcgtcgtaac cgtgagcttt ctgcagaagg ctttaacttc
    12301 attggtaccg gtgtctccgg tggtgaagaa ggcgcgctga aaggtccttc cattatgcct
    12361 ggtgggcaga aagaagcctg tgaactggtt gcgccgatcc tgaccaaaat cgccgcagtg
    12421 gctgaanacg gtgagccatg cgttacctat atcggtgccg atggcgcagg ccattatgtg
    12481 aagatggttc ataacggtat tgaatacggc gatatgcagc tgattgctga agcctattct
    12541 ctgcttaaag gtggcctgaa tctttccaac gaagaactgg cgcagacgtt taccgagtgg
    12601 aataacggtg aactgagcag ctacctgatc gacatcacca aagatatctt caccaaaaaa
    12661 gatgaagacg gttactacct ggttgatgtg attctggatg aagcagcaaa caaaggtacg
    12721 ggcaaatgga ccagccagag tgcgctggat ctcggcgaac cactgtcgct gattaccgag
    12781 tctgtgtttg cacgttatat ctcttctctg aaagatcagc gtgttgccgc atctaaagtt
    12841 ctctctggcc cgcaagcaca gccagcgggc gacaagggtg agttcatcga aaaagttcgc
    12901 cgtgcgctgt atctgggcaa aatcgtttct tacgctcagg gcttctctca gctacgcgcc
    12961 gcgtctgaag agtacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct
    13021 ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcttatgc cgaagatccg
    13081 cagatcgcta acctgctgct ggctccgtac ttcaagcaaa ttgccgatga ctaccagcag
    13141 gcgctgcgtg atgtcgttgc ttatgcagta caaaacggta tcccggttcc gaccttcgcc
    13201 gctgcggttg cctattacga tagctaccgt gccgctgttc tgcctgcgaa cctgatccag
    13261 gcccagcgcg ac
//
