LOCUS       GU068041               15588 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain W27 serogroup O115 O antigen gene cluster,
            complete sequence.
ACCESSION   GU068041
VERSION     GU068041.1  GI:307340767
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 15588)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="W27"
                     /db_xref="taxon:562"
                     /note="serogroup: O115"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADN43831.1"
                     /db_xref="GI:307340768"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADVCDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    108..15412
                     /note="O antigen gene cluster"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADN43832.1"
                     /db_xref="GI:307340769"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGILLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADN43833.1"
                     /db_xref="GI:307340770"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFVGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYVVTGLYFYDNDVVEMAKNLEPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLIKMIKGY"
     gene            3033..3581
                     /gene="rmlC"
     CDS             3033..3581
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADN43834.1"
                     /db_xref="GI:307340771"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYAAKHERCIQWKDGMLNISWPIG
                     NAPILSEKDRNGNGFLSVDFFG"
     gene            3588..4661
                     /gene="wegG"
     CDS             3588..4661
                     /gene="wegG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegG"
                     /protein_id="ADN43835.1"
                     /db_xref="GI:307340772"
                     /translation="MSSKLVIRENIAYYLPQYHRIPENDKWWGDGFTEWVQVKSAKKF
                     FPEHLIFHPEDNNYYDLTDISVIEKQYKLAKEHSITGFCFWHYWFGNGDTLLEKPAEM
                     ILNSDADVRFCFGWANHSWWNKKDNILLKEQRYGGEDEQKSHVEYLLPFFNDERYIKI
                     DGKPVFLIFKPYEIPDAKRWINKFRYIARAKGINELFIIGEFSKENDIEAYGLDAVVN
                     SRGMLQNRTLIEKIRDKLIRKNILNEDLFSPRRYSYSKLSKGFNTFVENSNRVIPVII
                     PRWDSTVRHGKNGWVLTGSTPKEFAKHVYDVKKILSKRDIKYRIAIVKSWNEWAEGNF
                     IEPDNIYGKRYLEILKSEFTNNG"
     gene            4654..5385
                     /gene="wegH"
     CDS             4654..5385
                     /gene="wegH"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegH"
                     /protein_id="ADN43836.1"
                     /db_xref="GI:307340773"
                     /translation="MVKNIFFTIYQLFGFAAIKRKILNFFFNIKNKKRYIKLSLNTNV
                     RKTYFESHIAVLGDAEVISSSIGRGTYIGGGCRIINTKVGRFCSIAKDVHIVSGNHPT
                     TDFISTHPMFYLSNNDTIINMGLNCLCKSKFNEYTYVDDQYVVNIGNDVWIGQGVMIL
                     NGVSIGDGSVIATGAVVTKDVPPFSIVGGIPAKVIKSRFSNKVIKDVSDTKWWNYDVD
                     FLMENVDSFESIEKFRSLSRHIKHD"
     gene            5378..6865
                     /gene="wzx"
     CDS             5378..6865
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43837.1"
                     /db_xref="GI:307340774"
                     /translation="MIKRNALSNLVGRIWGIISLFIFIPFYISYLGSTGYGYIGFYNT
                     LVALLAFADLGFSAATTREFARLSGGDEQNEIEKSKILRSYEFLYLSIVIIVVLTISF
                     GANWISHKWLVASTNVDVSQLIILMALSFGLQLPTNLYVGALMGSERQILANGLQIGW
                     GIFRSAGVLPILHYISNSLEAFFIWQFLSNFLFLIIIYKLTWRKIKSSDKKFSLNILK
                     STYRYALGMAGMGILASVATQIDKLIVTKNFNIEVVGFYTLAGTLSLIPLIFITTLAK
                     AAFPRFTILKEKQEIEHLKTLYFSLSKIAVVAILPISTVLAIYSHSIIYMWTHSLIIA
                     DKASDIVFFLMLAQAIQALTVLPFYLSLSYAYVRINMIFSAIMIIEIPIVYYFAMQHE
                     GTVGVAKSVLITVLTIFIPYMYLLHTRLISGIFSRWCSEVSISVFLCVIAIYILDRLG
                     VNDRDNLLFTVINGLVTWLILSIIILFGVGIKKPSHMFALLKSLR"
     gene            7673..8740
                     /gene="wzy"
     CDS             7673..8740
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43838.1"
                     /db_xref="GI:307340775"
                     /translation="MVLIILLSISMIIAFLIGIRQKDVCCYLIVCMSSFLFAFVVMCI
                     VFLLLKFNGNAELIFPDEMLYVNDSTSQLFFSRYVQFFDKNFSLDVVRWINITLFNIS
                     LAFLASEISSYLYGRTRLFCSLLAITGTIVGGYWAFFILKEAFSVAALSMLIVSYIRK
                     SKVFLVFASCLLFFARPELLLLYIAITIAFYFKRKNTHLYYLAIVTSMVCFILFMNSE
                     YSYSIKLFTLSRRFGESEFEFDDVAITTSNLSFFPFIMSEPFRQAVMTNINSTFNPFL
                     DLNPLVVVQRLYNIIGFYIFLTCVKPYLMKDKLYSFAFIVLLGVLFTHSVYRYFNTIL
                     IPFSLYFLFLKYRGLNKGSYK"
     gene            8737..9663
                     /gene="wegI"
     CDS             8737..9663
                     /gene="wegI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegI"
                     /protein_id="ADN43839.1"
                     /db_xref="GI:307340776"
                     /translation="MTILSIIIPVYNSSSCLEDALKSVVIQNLDSYEIIVVDDGSTKE
                     EHYACIDICKQYANTKLVTKENGGAASARNFGAKKARGILLAFLDSDDVWLKNKILSQ
                     IGYLKTQNADLVLGNIIVTDYFLNAKYKASKKISKNKYSLIKDIFYGKVIMNTPTILV
                     TKDMFIRVGGFDESLKYREDHSFLIDVVGNGNGKIVLDPAYNTLRRERENSLSSVCDI
                     YSELDKHMPFWLKFEKKYSFLITKKARKKLLSGLCLYYIRNERDADIKETLRCLKNDS
                     YFLYLCYRLLTNSSSILSLLYTLRNKLRNAAF"
     gene            9650..10210
                     /gene="wegJ"
     CDS             9650..10210
                     /gene="wegJ"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegJ"
                     /protein_id="ADN43840.1"
                     /db_xref="GI:307340777"
                     /translation="MLHFKILRLFNRVLFFFKFLLLKVFLGSKIQFPEFYRSCIDSGS
                     IISCVNGILIFKGRFISRRFLTINISSGKVIIGKNVFTNQNVSINCHTFISIGDNTII
                     GESVKFYDHNHRFNKDGCIKDQGFTSKPIIIGNNVWIGSSSVILNGVEIGNNSVISAG
                     SVIRSSIPENSIYKDGRVVNIMKKED"
     gene            10212..11405
                     /gene="wegK"
     CDS             10212..11405
                     /gene="wegK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegK"
                     /protein_id="ADN43841.1"
                     /db_xref="GI:307340778"
                     /translation="MRILYVSVERRYKLFNGRFFVQGIENVIFFERYLQCFDAVKVIA
                     RVEIVNSLPENHELFEHKAISIEPIFTVGTGLTSITRIYKLITSINKKDKVFIILRTP
                     GVLAYLLSLLCIARNIKFSIEVVTNPIQEAEHLSKNPIIRLLFRLVFLAVFKLQLRFT
                     QYASFVTEHEIQNKFLSGNKKLNVRFNSSYSSVVLQDDNFADEKIINARINRNKKNKN
                     IRLLFIGVLDRDFKGLDVFLNLISVLPEKYTATIIGDGLLLGKYKKIAEKLNVLTRVD
                     FCGYISSSIKKQQLMENADFFILTSRREGLPRVVIEAMAYGLPCVCTSVSGVNELIDK
                     KLIFPIDDYQAAKNILLSLDDESYADISIRNFRKSKKYGHFLLAEQRKLFYLKVISGE
                     SSSFK"
     gene            11380..12495
                     /gene="wegL"
     CDS             11380..12495
                     /gene="wegL"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegL"
                     /protein_id="ADN43842.1"
                     /db_xref="GI:307340779"
                     /translation="MVKVLHLSKYYKPYSGGIEQVVADISEGCLSKGIDSSVLAVNHK
                     TNSYEYDLNGVHVYGCKSNYHYASADVSISYIIKLRQIIESFDIIHIHLPNPLANLAL
                     FFSNYKNKKIIIHWHSDIVKQKKLKMLYMPLQQWLLKRADAIITTSPVYGEFSDDLRK
                     HKNKIKIIPIGINPENYKVDTNIVKKIHEQYKGKKIIFSLGRLVYYKGFEYLVESAKF
                     LPDDYIILIGGNGEEKQKLLELIQRYKLEDKVKLLGFIRHEELSSYFAASNVFCLPSI
                     EKSEAFGVVQLEAFLFNIPVVSCDIKGSGVGWVNKNNVSGIVVQPKFPYELADALKKI
                     ISDRDKYADVKKYFTENFHVNVMVERTIKLYKEIINI"
     gene            12509..13915
                     /gene="manC"
     CDS             12509..13915
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADN43843.1"
                     /db_xref="GI:307340780"
                     /translation="MSNDIIPVVLSGGSGSRLWPLSRQQYPKQFLSLTDERSLLQDTL
                     ERVKGLNYSQNIVICNEEHRFLVAEQLRESNFLQTSSIILEPTGKNTAPAIALAAFNL
                     LAEGNDALLLILAADHVITENEKFISAIKKAIPYANNGKLVTFGIKPLKPEIGYGYIY
                     KGDELEGNVFKIAKFVEKPTHDVAVSYLASEKYFWNSGIFLFSASAYLKQLRIYAPDI
                     YEQCRIAISQSVKDMNFIRPEPSTFSLCESISIDYAVMEHTEDGVMVITDMNWNDVGS
                     WSSLWDISPKCAQGNYIFGDVVHHNTSNSYIYSTNGLVATVGIDNLIVVNTKDALLVA
                     SKDKVQDVKTIYERLNQSGRIECLSHTVTYRHWGFYECLDKGVGFEVRKIVVKPKEKI
                     ELQQHFHRAEHWTVLKGTAQVTKDDKSYILTENESTYIPIGEVHSVLNPGSFPLEIIE
                     IRTGSYLFEDDNYHVENP"
     gene            13997..15412
                     /gene="manB"
     CDS             13997..15412
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADN43844.1"
                     /db_xref="GI:307340781"
                     /translation="MNTSDVIHNSGIEFGTSGARGLVIDFTHSTTVAFTLAFLIHLKK
                     EFVFKEVALAIDNRPSSFGIAKFCASAIINMGMTPIYYGVIPTPALACKAMAENMPAI
                     MITGSHIPFDRNGMKFYRPDGEITKKDEVEIISTSINFDLSLFKLPHLTCSPAAKNYY
                     IDRYVRLFPKNFLAGKHIGIYEHSSAGRDIYAELLCMLGAKVTSFGRSDEFVPIDTEA
                     VSQNDRDLAKIWSQDYELDAIFSTDGDGDRPLVADETGNWLRGDILGLISAIELGIEA
                     LSVPISCNTAIESTGKFTEVYRTRIGSPYVIEAFQDLSKNHKNIAGFEANGGFLLGSD
                     VSFGGHTISALPTRDAVLPLLMLFSATKTSCISSLVRALPERYTFSDRIKNFGRSKSD
                     ILLNYIRSNAPLFIHELGMAAKVINIDVTDGLRLILDNSDIIHLRPSGNAPELRCYAE
                     SDAVWKAEYLVTSVLKFLSEK"
BASE COUNT     4786 a   2407 c   3205 g   5190 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aagatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aagatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacttggaa tcacttgctg
      241 atgtttctga ttccgaacgc tatgtttttg aacatgcgga tgtttgcgat gcagctgtaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt gttggtacat
      421 atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct catcctgacg
      541 aagtaaataa taaagaacaa ttacccctct ttactgagac gacagcttac gcgccaagca
      601 gtccttattc cgcatcaaaa gcatccagcg atcatttagt ccgtgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaactgtt cgaataacta cggtccttat cactttccgg
      721 aaaaattgat tccactagta attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 tcgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta ccgaaagaga
      961 aatcttaccg cgagcaaatt acttatgttg ccgatcgccc gggacacgat cgccgttatg
     1021 cgattgatgc agagaagatt agccgcgaat tgggctggaa accgcaggaa acgtttgaga
     1081 gcgggattcg taaaacggtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     1261 gggtaatttg attgctcttg atgttcactc cactgattac tgtggtgatt ttagtaatcc
     1321 tgaaggtgta gctgaaaccg taagaagcat tcggcctgat attattgtca acgcagccgc
     1381 tcacaccgca gtagacaaag cagaatcaga accggagttt gcacaattac ttaacgcgac
     1441 gagtgtcgaa gcgatcgcga aagcagccaa tgaagtcggc gcctgggtta ttcactactc
     1501 tactgactac gtatttccgg ggaccggtga aataccatgg caggaggcgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agctggagaa aaagcattac aagagcattg
     1621 tgcgaagcac ctaattttcc gtacaagctg ggtctatgca ggtaaaggaa ataacttcgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gccgttatta atgatcagtt
     1741 tggtgcgcca acaggtgctg aactgctggc tgattgtacg gcacatgcaa ttcgtgtggc
     1801 actgaataaa ccagaagtcg caggcttgta tcatctggta gccagtggta ccacaacctg
     1861 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc tccttgcact
     1921 caacaagctc aatgcagtac caacaacagc ctatcctaca ccagcccgtc gcccccataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggtgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tactcgtctt tatcctgtga cgatggccgt
     2221 cagtaaacag ctgttaccga tttatgataa accgatgatc tattacccgc tctctacact
     2281 gatgttggcg gggattcgcg atattctgat tattagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg gtagccagtg ggggctaaat cttcagtaca aagtgcaacc
     2401 gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttgtcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatctt ctacggccac gatctgccga agttaatgga
     2521 cgtagctgtt aacaaagaaa gtggtgcaac agtatttgct tatcacgtaa atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca attagcctgg aagaaaaacc
     2641 gctacagcca aaaagtaatt atgtggtaac tgggctttat ttttatgata acgacgttgt
     2701 cgaaatggcg aaaaatcttg agccttctgc ccgtggtgaa ctggaaatta ccgatattaa
     2761 ccgcatttat atggaacagg ggcgtttatc cgttgccatg atgggacgtg gttatgcatg
     2821 gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg caacaattga
     2881 agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gcttaccgta aagggtttat
     2941 tgatgctgag caggtgaaag tattagctga acctctgaaa aaaaatgctt atggtcagta
     3001 cctgataaaa atgattaaag gttattaata aaatgaatgt tattaaaaca gaaatcccag
     3061 atgtattgat ttttgaaccg aaagtttttg gtgatgagcg tggtttcttt tttgagagct
     3121 ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtc caggataacc
     3181 attcgaagtc ttgtaaaggt gttttacgcg gactgcatta tcagttagag ccttatgcac
     3241 aagggaaact tgtacgctgt gttgtcggtg aagtttttga tgtcgcggtt gatattcgta
     3301 aatcgtcacc tacctttggc aagtgggttg gtgtaaattt gtcggcggag aataagcgcc
     3361 agttgtggat cccggaaggg tttgcgcatg gttttttggt gttgagtgag acggctgaat
     3421 ttgtttataa gacgacgaat tattacgcag cgaaacatga acgatgtatt caatggaagg
     3481 atggcatgtt aaatatctct tggccaattg gaaatgcccc tatactttcc gaaaaagaca
     3541 gaaatggtaa tggctttcta agtgttgatt tttttggttg ataaaatatg tctagcaagt
     3601 tagttattag agaaaatatc gcttattatt tgcctcaata tcatcgtatc cctgaaaatg
     3661 ataaatggtg gggcgatggg tttaccgagt gggtacaagt aaaatctgcc aagaaattct
     3721 tcccagaaca tttgattttc catccagagg ataataacta ttatgatctc actgatatat
     3781 cagttattga aaaacaatat aagttagcta aagaacattc gataactggt ttctgttttt
     3841 ggcattactg gtttggtaat ggcgatacgt tattagaaaa accagcagaa atgatattga
     3901 atagtgatgc tgatgtacgt ttttgttttg gttgggctaa tcactcatgg tggaataaaa
     3961 aagacaatat tcttttgaaa gaacaacgct atggagggga agatgagcag aaaagtcatg
     4021 tagagtattt attacctttt tttaatgatg agcgctatat taagatagat gggaagcctg
     4081 tttttttaat tttcaaacca tatgagatac ctgatgcaaa aaggtggatc aataaatttc
     4141 ggtatatagc aagagctaaa ggaattaatg aattgttcat tattggtgaa ttttcgaaag
     4201 aaaacgatat tgaagcatat ggactagatg cggttgttaa ctctcgagga atgttacaga
     4261 atagaacact catagaaaaa atacgggata aattaattcg taaaaatatt ttgaatgaag
     4321 atttatttag cccaaggaga tattcttata gcaaactttc taaaggattt aatacatttg
     4381 tagaaaattc taatagagtt attccagtca taatacctag gtgggattca accgttcgtc
     4441 atgggaaaaa tgggtgggtg ctaacaggat caacaccaaa agaatttgct aagcatgttt
     4501 atgatgtaaa gaaaatccta agtaaaagag atataaaata ccgaattgcc atcgttaaat
     4561 cgtggaacga atgggctgaa gggaatttta ttgagccaga taatatatat ggaaaaagat
     4621 atctagaaat acttaaaagc gagttcacaa ataatggtta aaaatatttt ctttacaata
     4681 tatcaattgt ttggatttgc agcaataaaa agaaaaattt taaacttttt tttcaatatt
     4741 aagaataaaa agagatatat aaaattatcg ctgaatacaa atgtcaggaa gacttatttt
     4801 gaaagccata tagctgtatt aggcgatgca gaagttatat catcatcaat tgggcgtggt
     4861 acttatattg gtggaggttg tcgaattata aatacaaaag taggacgttt ttgctcaatt
     4921 gcaaaagatg tacatattgt atcaggaaat catcccacaa cggattttat atctacccat
     4981 ccgatgtttt atttaagtaa taatgatacc attattaata tgggattaaa ttgcttatgc
     5041 aaatcaaaat ttaatgaata cacttatgtt gatgatcaat atgttgtgaa tattggtaac
     5101 gatgtttgga tcggtcaggg agtcatgatt cttaatggtg tttctattgg tgatggttct
     5161 gttattgcaa ctggggccgt tgtgactaaa gatgtacctc cattttctat agtaggtgga
     5221 ataccagcta aggttataaa atcaagattt tcaaataagg ttattaaaga tgtatctgat
     5281 acaaagtggt ggaattatga tgttgatttc ctaatggaga atgtagattc ttttgaatca
     5341 attgaaaagt ttaggtcttt gagtcgacat ataaaacatg attaaacgta atgcactgtc
     5401 aaatttagtc ggtaggattt ggggaattat atctttattt atatttatac ctttttatat
     5461 atcatattta ggcagtactg gctatggcta tattggtttc tataatacat tagttgcgtt
     5521 gctagctttt gctgatttag gtttttcagc agcaactaca cgtgaatttg ctcgcctctc
     5581 tggtggtgat gaacaaaatg aaatagaaaa gtcaaaaata ttaaggagtt atgagtttct
     5641 atatttatca attgtaataa tagttgtgct tacaatatca tttggagcga attggatttc
     5701 gcataaatgg ttggtagcat ctacaaatgt cgatgtgtct caattgatta ttctcatggc
     5761 attgagtttt gggttgcaac tccctacaaa tctgtatgta ggggcactaa tggggagtga
     5821 aagacaaata ttagcgaatg gattgcaaat tggctggggc atatttcgta gtgcaggggt
     5881 attgcctata ttgcattata tttctaatag tctagaggcc ttttttatct ggcaatttct
     5941 atccaatttt ttatttttga ttataatata taagctaaca tggaggaaaa ttaaatctag
     6001 cgataaaaaa ttctcattaa atattcttaa atctacatac aggtatgctt taggaatggc
     6061 tggtatgggg atattggctt cggttgcaac acaaattgac aaactaatag tgacaaaaaa
     6121 ttttaacatt gaggtggtag gattttatac cttagctgga acgttatcat taatcccttt
     6181 gatatttatt accacattag caaaagcagc ttttccacgg tttacaatac tcaaagaaaa
     6241 gcaagagata gagcatttaa aaactcttta ttttagtctc agcaaaatag ctgtcgttgc
     6301 gatattacct atatctacgg tattggcaat ttattctcat tcaattattt atatgtggac
     6361 acactcttta atcattgccg acaaagctag tgatattgtt ttttttctga tgctggctca
     6421 agctatacaa gcattaactg tattgccatt ttatttgtct cttagttatg cttatgtcag
     6481 aattaatatg attttttcag ccatcatgat tatagaaatc cccattgttt attatttcgc
     6541 catgcaacat gaaggaactg tcggtgtggc taaatccgtg cttatcacgg tattaactat
     6601 atttataccc tatatgtatt tattacatac tcgtcttatc tctggtatat tttctagatg
     6661 gtgttctgag gtttcaattt ctgttttttt atgcgtgata gctatttata ttttagatag
     6721 gttgggtgtt aatgataggg ataatctttt atttacagtc atcaatggat tagttacatg
     6781 gctaatattg agtattataa ttttatttgg tgtggggatt aaaaaacctt ctcatatgtt
     6841 tgctcttttg aagtcattaa gataattggt aatgtctcta attggttgaa tgtgatgtat
     6901 aatgcacgct tttgaggttt actcatggcc agcgttgccg ttcattgtcc ccgttgtcag
     6961 tctgttcagg tttatcgtca tggacagaat ccgaaaggac atgcccggtt tcgtcgccgt
     7021 gactgccacc gcgtcttcca gctcacttat acttatgaag cccgcaagcc cggggttaaa
     7081 gagcagatca ccgtaatggc tttcaacgga gccggggttc gtgataccgc cagaacactg
     7141 aaggtcggca tcaacaccgt tatccggact ttaaaaaact ctcaccacga cgagtaacat
     7201 catctccggt agcccatgcc gatgtggcgc ttatctgtga acttgatgag cactggagct
     7261 tcgttggcag caaggcgcaa caacactggc tctggtacgc gtacaacaca aaaacgggtg
     7321 gtgtactggc ctacactttt ggtccacgta ccgatgaaac ctgtcgtgaa ttgctggcac
     7381 tgctcacgcc ctttaacatc ggaatgctga ccactgatga gtgggggagc tatgccaggg
     7441 cgcttccgaa agaaaagcac ctgaccggca agatttttac tcagcgcatt gagcgcaata
     7501 acctgacgct gagaacccga atcaaacggc tggcccgcaa aacgctctgt ttttcacgtt
     7561 ctgttgagct tcatgaaaaa gtcatcgggg cattcatcga aaaacacatg ttctactaat
     7621 tggatacatc accagataat taatagtaat aatatcctga agagataact acatggtctt
     7681 aataatattg ttaagtataa gtatgataat cgctttcctt ataggtataa ggcagaagga
     7741 tgtttgctgt tatttaattg tatgcatgtc ttctttttta tttgcattcg tcgtgatgtg
     7801 cattgttttt ctattattaa aattcaatgg aaatgcagaa cttattttcc cagacgaaat
     7861 gttatatgtg aatgactcaa catcacaact ttttttctct agatatgtac aattttttga
     7921 taaaaatttc agtttagatg ttgtccgatg gattaatata acgctgttta atatatcatt
     7981 ggcattttta gcttcagaaa tctctagtta tttatatgga agaacccgat tattttgttc
     8041 gttattagct ataacgggaa caattgtcgg cggctattgg gcttttttta ttttaaaaga
     8101 ggcgtttagt gttgcagctt tatctatgct tatcgtttct tatattcgaa aaagcaaggt
     8161 gtttttggta tttgcatcgt gtcttttatt ctttgctcgg cctgaacttc ttttgttata
     8221 catagctatt actatcgctt tttattttaa aagaaaaaat actcaccttt attatttagc
     8281 tatcgtaaca tccatggtat gttttattct atttatgaat agtgaatatt cttattctat
     8341 taaacttttt actctatcaa gacgctttgg ggaatctgag ttcgaattcg atgatgttgc
     8401 tattactact agtaatttgt catttttccc tttcattatg tcagaaccat ttaggcaagc
     8461 ggtgatgaca aatataaata gtacatttaa tcctttctta gatttgaatc ctctggtagt
     8521 agttcaaaga ctttacaata taataggttt ttatattttc ttaacttgtg ttaaacctta
     8581 tcttatgaaa gataaactgt acagttttgc ctttatagtt ttacttggtg tgttatttac
     8641 acattccgtt tatcgatatt ttaatacaat attaatacca ttttctttat atttcttatt
     8701 tttgaaatat aggggcttaa ataaagggag ctataagtga ccatactatc tattatcatc
     8761 cctgtttata attcgtcaag ttgcttagag gacgcattga aatcagtggt catccagaat
     8821 ttggattcat atgagattat cgttgtcgat gatggttcaa ctaaggaaga gcattatgcc
     8881 tgtattgata tttgtaagca atatgctaac acaaaactgg tcacgaagga aaatggtggt
     8941 gctgcttctg ctaggaattt tggtgcaaaa aaggcaagag gaatattgct agctttcctt
     9001 gatagtgatg atgtttggct taaaaataaa atattatcgc agattggtta tttgaaaacg
     9061 caaaatgcgg atttagtatt gggaaatata attgtcactg actattttct gaatgcaaaa
     9121 tataaggcaa gtaagaagat atcaaaaaat aaatattctc ttattaaaga tatattttat
     9181 ggtaaagtta ttatgaacac tccgactata cttgtcacaa aagatatgtt tataagagtt
     9241 ggtggttttg atgagtcttt aaagtaccgt gaggatcatt catttcttat tgatgttgtt
     9301 gggaatggta acggtaaaat tgttttagat ccagcctata atactttacg tagagagaga
     9361 gagaatagtt taagctcagt ctgtgatata tattcagaac ttgacaagca tatgccattt
     9421 tggctgaagt ttgaaaaaaa atatagcttt cttataacta aaaaagcgcg aaagaaatta
     9481 ttatcaggtt tgtgtctata ttatatacga aacgaacgtg atgcagatat taaggaaact
     9541 cttaggtgtt taaagaatga tagttatttc ttatatcttt gctataggct cttgaccaat
     9601 tcgtcatcaa tactttcttt attgtatact ttaaggaata agttgcgaaa tgctgcattt
     9661 taaaatactg agattattta acagagtgtt gttttttttt aaatttttac ttttgaaagt
     9721 ttttttgggt agtaaaattc aatttcctga gttttaccga tcatgtatcg attcaggttc
     9781 tattatttca tgtgtgaatg gaatattgat ttttaaaggt cgttttatta gccgtagatt
     9841 tttaaccatt aatatttctt ctgggaaagt aataataggg aagaatgtat tcaccaatca
     9901 aaatgttagt atcaattgtc atacttttat aagtattggt gataatacga tcattgggga
     9961 atcagttaaa ttctatgatc acaatcatcg gtttaataag gatggttgta ttaaagacca
    10021 agggtttact tcaaagccta ttattattgg aaataatgtt tggattggtt ctagctcagt
    10081 tatattaaat ggggttgaaa ttggtaataa ttctgtaatt agtgcgggga gtgttattcg
    10141 cagttcgatt cccgaaaatt ccatatataa ggatgggcga gttgttaata ttatgaaaaa
    10201 ggaagattga catgcggatt ttgtatgttt ctgtagaaag gagatacaaa ttatttaatg
    10261 gacgattttt cgttcaggga attgagaatg taatattttt tgaacgatat ttacaatgtt
    10321 ttgacgcggt gaaagtcatc gcccgtgttg aaattgttaa ttcattgccg gagaatcatg
    10381 aactatttga acataaagca attagtattg aaccaatatt tacagtgggt actggcttaa
    10441 cttcaattac tcgaatatat aagctaatta caagtataaa taaaaaagac aaagttttta
    10501 tcattctacg tacacctggt gttttagcat atttattgtc tttattatgt atcgctagga
    10561 atataaaatt tagtattgag gttgtaacta accctattca agaagcagaa catttatcta
    10621 aaaatccaat aattcgactc ctttttcgct tggtgttttt agctgtattt aaattgcagt
    10681 tgagatttac tcaatatgca tcttttgtta cggaacatga aatacaaaat aaatttctga
    10741 gtggaaataa aaaattaaat gtgcgtttta attcgagtta tagtagtgtt gttttacagg
    10801 atgataattt tgctgatgag aaaataatta atgctcgcat caatcgaaac aaaaaaaaca
    10861 aaaacattag attgcttttt attggtgtat tggatcgtga ttttaaaggg cttgatgttt
    10921 tccttaacct tatatctgtt ttgccagaaa aatatactgc aactatcatt ggcgacggat
    10981 tgttattggg taaatataaa aaaatcgctg agaaattaaa tgttttaacg cgcgttgatt
    11041 tttgtggtta tatttcctca tctatcaaaa aacagcaact aatggaaaat gctgattttt
    11101 ttattttaac atctcggaga gaaggactac ctcgggtagt catagaagca atggcttatg
    11161 gtttaccgtg tgtttgcaca tctgtttcgg gagttaatga gttaattgat aagaaactca
    11221 tttttccgat tgatgattac caagcagcaa aaaatatttt attaagcctt gatgatgaaa
    11281 gttatgctga tattagtata aggaactttc ggaaatctaa aaaatatgga cattttttac
    11341 ttgcagaaca aagaaaatta ttttatttaa aggtgattag tggtgaaagt tcttcattta
    11401 agtaagtatt ataaacccta ttctggcgga atagagcaag ttgtagctga tataagcgaa
    11461 ggatgcttat caaaaggtat cgattcttca gttttggcag ttaatcataa aactaactct
    11521 tatgaatatg atctaaatgg tgtacatgtt tatggctgta aatcaaacta tcattatgcc
    11581 tctgcagatg tatctatttc ttatattata aaattaagac agataatcga atcattcgat
    11641 attattcaca ttcatttacc caaccccttg gcaaacttgg cattattttt ttctaattat
    11701 aaaaataaga aaataattat tcattggcat agtgatattg ttaaacagaa aaaattaaaa
    11761 atgttatata tgccattgca acaatggtta ttaaaacgag ctgacgctat tattaccaca
    11821 tcaccagtat acggtgaatt ttctgatgat ttgagaaaac ataaaaataa gatcaagatt
    11881 attcctattg gtattaatcc agagaattat aaagttgata ctaatatagt taaaaaaatt
    11941 catgaacagt ataaaggaaa aaagattatt ttttcattgg ggagattagt ttactataag
    12001 gggtttgaat atctggtaga atccgcaaaa ttccttcctg acgactacat tattctcata
    12061 ggtgggaatg gagaggaaaa acagaagctt ttagagctca ttcaacgata taaacttgag
    12121 gataaggtta aattacttgg atttattaga catgaggagt tatcatctta ctttgctgcg
    12181 agcaatgttt tttgtttacc ttcaattgaa aaatctgagg cttttggagt tgttcaactt
    12241 gaagcattcc tctttaatat cccggtagtt tcatgtgata taaaaggttc tggtgtaggt
    12301 tgggttaata agaataatgt ttccggtatt gttgtgcaac caaaatttcc atatgagctt
    12361 gccgatgcat taaaaaaaat aatatcagat cgagataaat atgctgacgt taaaaaatat
    12421 tttacggaaa atttccatgt taatgtgatg gttgagcgta caataaaatt atataaagaa
    12481 attattaaca tatagtaggt catagataat gagtaacgat attatcccag ttgttttgtc
    12541 aggtggttcc ggttcaagat tatggcccct gtcacgacag cagtacccta aacagttttt
    12601 atctcttact gacgaaagga gtttacttca ggacacttta gaacgtgtta aaggtcttaa
    12661 ttattctcaa aatattgtca tttgcaacga agagcatcgt tttcttgttg ctgaacagct
    12721 ccgagaaagt aatttcttac agacatcatc aattattctt gaacctacgg ggaaaaatac
    12781 tgctccggct attgcattgg ctgcgtttaa tttgttagca gaagggaatg acgctctttt
    12841 actgatcctt gcagcagatc atgttataac agaaaatgaa aaatttattt ctgcaataaa
    12901 gaaggctatt ccttatgcta ataatggaaa attagtgact tttggtatta aacctttaaa
    12961 gccggagatt ggatatggtt atatttataa aggtgacgaa cttgagggta atgtattcaa
    13021 aattgcaaaa tttgtcgaaa aaccgactca tgatgtagct gtatcttatt tagcatctga
    13081 gaagtatttt tggaatagtg gtatatttct atttagcgct tctgcatatt taaaacaatt
    13141 gcgtatctat gctcctgata tttatgaaca atgccgaatt gctataagcc aatctgtgaa
    13201 agatatgaat tttatccgac cagagccaag taccttcagt ttgtgtgagt caatatcgat
    13261 tgattatgct gttatggagc atacggaaga tggtgttatg gttattaccg atatgaactg
    13321 gaatgatgtg ggatcgtggt cttctctatg ggatatatct ccaaaatgtg ctcaaggtaa
    13381 ttatatattc ggtgatgtgg ttcatcataa cacttcgaat agttatatct attcaacaaa
    13441 tggactagtt gctactgttg gtattgataa tttaattgtt gtaaatacta aagatgcgtt
    13501 attggttgcg agtaaggata aagttcagga tgtcaagacg atttatgaga gactcaatca
    13561 atctggacgc atagagtgtc tttcgcatac agttacctat cggcattggg gattctatga
    13621 gtgccttgat aaaggagtcg gttttgaggt gaggaaaata gttgttaaac caaaagagaa
    13681 aatagaactc caacagcatt ttcatcgagc tgagcattgg actgttttga aaggcacagc
    13741 tcaagtgacg aaggatgata aatcctatat cctcactgaa aatgaatcaa cttatatacc
    13801 tattggtgag gttcatagtg tgcttaaccc gggaagtttt ccattagaaa ttattgaaat
    13861 acgaacgggc tcttaccttt ttgaggatga taattatcat gtggaaaatc cttagtcggt
    13921 caatcagata attaaaaatt agcgctatta atttaattgg tggttgttat aattatcttg
    13981 gtgatatttg catataatga atacttcaga tgtaatacat aactctggta ttgaatttgg
    14041 aactagtggt gctagaggtt tggttattga ttttactcat agtaccactg ttgcctttac
    14101 attggctttc ttaatacatc taaaaaaaga gtttgtattt aaggaagttg ctctcgcaat
    14161 agataatcgt ccgagcagtt tcggtatagc caaattttgt gcttctgcta ttattaacat
    14221 gggtatgacc ccaatatatt atggtgtcat acccactcca gctttagctt gtaaggcaat
    14281 ggctgagaat atgcctgcga ttatgataac aggtagccat ataccttttg acagaaatgg
    14341 aatgaagttt taccgtcctg atggtgaaat tacaaagaaa gatgaggtag aaataatttc
    14401 tacttccatc aattttgatt tatcactatt taaacttcct catctaacct gttctcctgc
    14461 agctaaaaat tactatattg accgctatgt acgtttgttt cctaaaaatt tccttgctgg
    14521 aaaacatatc ggcatatatg agcattcgag tgcaggtcgt gatatttatg cagaactctt
    14581 atgtatgcta ggtgcaaaag ttacttcttt tggtcgaagt gatgaatttg ttccaataga
    14641 tactgaggct gtaagtcaaa atgatcgtga tctggcgaaa atatggtcac aggattatga
    14701 gttagatgcc atattttcta cagatggtga tggtgatcgc cctttagttg ctgatgagac
    14761 tggtaattgg ctaagaggtg atattcttgg ccttataagc gccatagagc taggcatcga
    14821 ggcgttgtct gtgcctataa gttgtaatac tgccattgaa tctactggta aatttactga
    14881 agtttatagg actcgtattg gttctccata tgtgattgag gcgtttcaag acctgagtaa
    14941 aaatcataaa aatattgcgg gattcgaagc gaatggtggt tttttattag gtagcgatgt
    15001 tagtttcgga gggcatacta tttctgcatt accaacgcgc gatgccgttc ttccgttatt
    15061 aatgctattt tctgcgacga aaacatcatg tatctcatca ttggttaggg cattgcctga
    15121 acgctataca ttttctgatc gtataaaaaa ttttggaaga agtaaaagtg atatactgtt
    15181 aaattatatt cgtagtaatg cccctctctt tattcatgag ttaggtatgg ctgcaaaagt
    15241 tatcaatatt gatgtaactg atggattaag attgatattg gataacagtg atattattca
    15301 tttgcggcca tcaggaaacg cgcctgaatt acgttgttat gctgaatctg atgctgtatg
    15361 gaaagcagaa tacttggtaa catcagtttt gaaatttttg tctgaaaaat agttattcag
    15421 ttcttagatg tcatcaggtc gttgtcatgc aaattcgttg aagcattacg ttaatgcgat
    15481 gcctctttag tatcatttga ggtaatatct atattgtatt tctaatctgc cccctgacag
    15541 gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta tggcagtg
//
