LOCUS       AB812051               20297 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 28w.
ACCESSION   AB812051
VERSION     AB812051.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 20297)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="28w"
                     /db_xref="taxon:562"
                     /note="serogroup:O116"
     gene            <1..885
                     /gene="wcaM"
     CDS             <1..885
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01555.1"
                     /translation="GFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIER
                     INCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVI
                     RNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSI
                     PQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMR
                     NIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVN
                     HHIVNVEKINFRLPERRE"
     gene            1048..1953
                     /gene="galF"
     CDS             1048..1953
                     /gene="galF"
                     /note="O116_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01556.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2326..3402
                     /gene="rmlB"
     CDS             2326..3402
                     /gene="rmlB"
                     /note="O116_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01557.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHVDICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KKLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            3399..4265
                     /gene="rmlA"
     CDS             3399..4265
                     /gene="rmlA"
                     /note="O116_03"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01558.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQHGFKRLLGDGSSLGINLQYEVQNSPDGLAQAFIIGEKFIANDSV
                     CLVLGDNIFYGQGFSPKLKEASIRAKEKATIFGYQVKDPERFGVVEFDNQRNVLSIEE
                     KPSNPKSNYAVTGLYFYTNEVVEYAKLVKPSSRGELEITSINQMYLENGKLAVEMLGR
                     GFAWLDTGTHESLLQASQFVETIETRQGFKVACLEEIAFANGWLTKKELLIRSSLMSK
                     NSYGQYLLQLIK"
     gene            4272..5699
                     /gene="wzx"
     CDS             4272..5699
                     /gene="wzx"
                     /note="O116_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01559.1"
                     /translation="MAELKYKTINSLKWSALERIFSQGIQLILMLVLARLLGPKAFGL
                     IGMLAIFIAIGQVFIDSGFSSALIRKNDRTHIDYTTAFYFNVTVAILFYVVLFFSAPL
                     IAKFYHQPELELLSKIIGLSLITNSFCVVPRTILTIAMDFKSQAKVSVLSVVLGGAFA
                     LFLAWKGFGVWSLVAQTLTVSMCNALLLNIITKWIPGLFFSKKSFLYLFGFGGKLLIS
                     GLLDTIYNNMYQIIIGKKFSAANVGQYTQANQISSIPAMTLTNIIQRVTYPMFSHMQD
                     DEDKLKKNYLLTLKLAALFIFPIMAGIGIIAQPLFFIILGPQWIDASRLIIILSFAYM
                     IYPIHAVNLNILQVKGRSDLFLQLEIMKKLIGVIVILITMNYGIYGMCFGLVFHSYLC
                     LIVNTYYTKKMTKLTLKQQMIELGPVWIITIISCAIAYIGALHISDDALLQVLLMVVI
                     AVVLYIGLIFLLQRNLLKLALSIRK"
     gene            5711..6811
                     /gene="vioA"
     CDS             5711..6811
                     /gene="vioA"
                     /note="O116_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-xylo-4-hexulose aminotransferase"
                     /protein_id="BAQ01560.1"
                     /translation="MKKIPVTQPFLPDLNEFIPYLEKIWENKWLTNNGPFHQQLEKEL
                     CKYLGVEYVSIFNNATIALITALQALRITGEVITTPYSFVATSHSILWNGLKPIFVDI
                     DPETFNIDPKKIEEAITPETTAIMPVHCYSNPCEVEAIQEIADNYGLRVIYDAAHAFG
                     VNYKGRSLLSYGDMSVLSFHATKVFNTFEGGAIICQDAKLKQRIDRLKNFGIADELTV
                     TAPGINGKMSELNAAFGLVQLKHIDTAIQQRQKIDHMYREELSSVNGIRMIEYVSAAN
                     SNFSYFPILIEKNYRMSRDELYTELKKNGILSRRYFYPLISNMSMYRGIETATKEHLP
                     NANEIADKVLCLPIFNELTDNDFSRIIKTIRG"
     gene            6813..7379
                     /gene="vioB"
     CDS             6813..7379
                     /gene="vioB"
                     /note="O116_06"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-viosamine N-acetyltransferase"
                     /protein_id="BAQ01561.1"
                     /translation="MAFLKEEDVECLGFGSVGYNVLISPKASFYGAENIHIGNNVRID
                     DFCVFSAGKGGIYIGNYIHIAVYSSLIGSEKIVLSDYSNISSRVSIYSSNDDYSGRFM
                     SNPMIPAEFTNVTHAPVIIGKHVIIGSGSIVLPGVSINDGAVIGALSFVKRDCDEFTL
                     YAGNPLKRIKERQRNIERLELDFRGKFE"
     CDS             7409..8380
                     /note="O116_07"
                     /gene="wfeB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01562.1"
                     /translation="MFGMNSKVVISICCITFNHADYIEKCIEGFLAQNFPHKMEIIIG
                     DDLSSDGTREKLQKIQLQHPDLIKVIYNQENMGANKNILSVLRQAKGEFIAFCEGDDY
                     WIHEDKLYLQYKLLKTNPSVDFCFHSAYMDSEGEKKLHFKYGERTKKFYINDVLKVAG
                     QFAPTSSYMFRRNIIYALPAWFPQSCIGDLFMEIYSMKKGGIYIPLPMSVYRVNSVGS
                     WSETIKSNLNVFTEKHLNIATHLRLAKQEFNGYEKYFNLKIANVYLNMCTRFIIEKEY
                     KLFKVYLEKANGINEHFTRKQLLYNGLRFFPTIIYYIHYLKAIIKNK"
     gene            8388..9548
                     /gene="wzy"
     CDS             8388..9548
                     /gene="wzy"
                     /note="O116_08"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01563.1"
                     /translation="MYWIYCLFLLFIVSLLNKGRYSTLAYKASLLIIFIFATGNYYNG
                     VDWINYQKHYEFISNSGISSEYFAYEPGFLLLMYIFGNVLDIKNFHVIVIFISSISIL
                     SLSKFIKQIPKGVNKSLILFMLVAFIYPLYNDAIRQLFAFCIILPLLPNIKSIAIKKI
                     IITCGIAALFHSSIILMVPLFILMRRELTQKFMFYSFICALLFLSLLINIEFIVNTLN
                     NLIPKMLYVKLVNYVNNMGEFKVGFFAVIDFLGICIFCATYRVFKSNNDISVYAIGTF
                     YFFILHLVFYGAPFLQRLLYYIFPLLSVYSCFIFSHCQRGSGARILLPLTLIMTLSVF
                     ARNIFNPYYATDFYEPKFFYTDGVFEKHLDVERLKYRKCSVIDEYDPNFCPK"
     CDS             9551..10633
                     /note="O116_09"
                     /gene="wetP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01564.1"
                     /translation="MNILILSDEYYPYHIGGAGVVVSQIEKILLSKGEKYQIICSKPK
                     NKLLCEIYKLTWPLWYFYALVLITFKRYDRIIVNDVRSAYILGLFGRIATLKKCVYLV
                     HGTEVDIVFKSRSLKNSFILFPFFYKRFIKKIKKIVFVSKFVASRTNKELLRNNITPN
                     KQCVCYAGLGPEIYHLAATDDLYLEDENIDQIRLVSFSRLEKRKGYMEMIEIFNALIK
                     KGHSIHWDIYGDGSLKYDILKKIKQLELDNDIILLGKIDRNNLYKYINPNKYDAYWLL
                     PNEPEAFGLTYIESSAMGLPVFGPKKYGIEEAIKENENGFFFEGNNKFIQDLNKIKAN
                     KCKYIQSSKQWALCFDVEKFVNELVS"
     CDS             10640..11728
                     /note="O116_10"
                     /gene="weiC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01565.1"
                     /translation="MMKIVYVITGLSMGGAENQVCLLADQLTKLGHDVTLISLDNTLE
                     VKPINDKVKLRQLSIKKNPISLIINYFKLRKIIKKISPDVVHSHLFHANIICRLLKLT
                     MKIKKLISSSHSRYEGGVIRMLIYRVTQNLADIVTNVSLDASNELLKRRVVTENKLVT
                     VFNGIDCNKFKFIPDSRQDVFNEFSFSRDIKLLLAVGRFTEAKDYKTLLNAFKILISK
                     DDKFRLLVAGNGELFDSISTLKSQLNLDSFIYFLGVRDDIPRLMSAADIFVMSSAWEG
                     LPLVIGEAMSCNCLIVTTNCGGVKEILGECGQIVPIRSPDLLAEKIMWTMELDDEERG
                     ILKKNAQNRIHDRFSIDSIVAKWLELYS"
     CDS             11735..12853
                     /note="O116_11"
                     /gene="wcmT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01566.1"
                     /translation="MKKKIIAITANTSWYIYNFRRNTILTLIEQGYEVIACAPLDKYS
                     SYIEKLGCVHVPLIMDQNGKNPLKDLLTLFCFYKVFKKYKVDVVLNFTPKNNIYATIV
                     AKLLNIKVINNIAGLGTAFSSKNFLNLVAINLYKYSQKKADFIFFQNKEDAIIFKSFG
                     INESNTDILPGSGVDLRRFNVCPVHTDNVVRFALVARMLVDKGVMQYIECAENLKSKY
                     GNRVEFLLVGFLDDNNPRAVHSSIIEKWTNKDIVKYLGVSDSIEDIVSNVDCVVLPSF
                     YREGVPKSLLEAAAMGRPLITTDSIGCKETVINNVTGYLCEPRSVSDLCAKMEKIISM
                     SKEDKVKMGLAGRQYIERNFDERIVVDKYLNVISDFFE"
     CDS             12867..13886
                     /note="O116_12"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ01567.1"
                     /translation="MTLMTVLVTGGAGYIGSHTIIDLLENNYQVVAIDNLSNSSFESI
                     KRIKEVTNRHFDFYKVDLRNKKELDDIFLKHDISCVLHFAGLKSVSDSIKNPLNYYDN
                     NVFGTLNLLSLMIEHCVYRLIFSSSATVYGVPEKIPVTENCQTGGTTNPYGYSKLMIE
                     QIIKDITMSDSNWTAISLRYFNPVGAHPSGLLGESPIGIPNNLVPYITQVASGKLTKL
                     NVYGSDYNTKDGSGVRDYIHVLDLASGHTAALNKIGDLSGFNAFNLGTGKGYSVLELI
                     SCFEKVNGVKVPFTIAERRDGDVAECWSSPNLANAVLKWKAKYSLEDMLKDAWRWECN
                     NPNGY"
     gene            13998..15404
                     /gene="gnd"
     CDS             13998..15404
                     /gene="gnd"
                     /note="O116_13"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01568.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15652..16818
                     /gene="ugd"
     CDS             15652..16818
                     /gene="ugd"
                     /note="O116_14"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01569.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPRTNYFNTSSVETVIR
                     DVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAQRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDSRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(16884..17888)
                     /note="O116_15"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01570.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            18240..19274
                     /gene="wzz"
     CDS             18240..19274
                     /gene="wzz"
                     /note="O116_16"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01571.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(19370..19981)
                     /gene="hisI"
     CDS             complement(19370..19981)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01572.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6215 a   3554 c   4286 g   6242 t
ORIGIN
        1 ggatttcata accagattat cggtgccaac atcaccaatt gtaagttcag cgacttacag
       61 ggcgacgcca ttgaatggaa cgtggcaatt aacgacagtg atattttgat ctccgaccat
      121 gtcatcgagc gcatcaactg tactaacggc aaaatcaact ggggcattgg cataggcctt
      181 gcgggaagca cttacgataa taattacccg gaagatcagg cagtgaaaaa ctttgtcgtg
      241 gcgaatatca cgggatcgga ttgtcggcag ttgatccatg ttgaaaatgg taaacatttt
      301 gttattcgta atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt
      361 gataacgcga cagtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg
      421 attaatagcg ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata
      481 ccgcaaaatt tccgagtgaa taatattcaa ctggataata cccaccttgc ttataaattg
      541 cgcggtatcc aaatctccgc cgggaatgcc gtctcctttg tgtcgctaac taacattgag
      601 atgaagcgtg cctcgcttga gcttcacaat aaaccgcaac acctttttat gcgaaatatc
      661 aaggtgatgc aggaatcctc agttggacca gcattgagca tgaacttcga catgcgcaaa
      721 gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactgc tgtcacttgc aaatgttcat
      781 gcggtgaatg aaaaagggca aagctccgtc gatatcgaca gagttaatca ccatattgtt
      841 aatgtggaaa agattaactt tagattgccg gaacggaggg agtagatttg cgaccattcc
      901 tggaaaaatg gagccatact taggaacaat gctactgcaa tccacaacga agcggcgtaa
      961 catcacaagt aattcagtaa tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca
     1021 agctataatt cagcaaccat tttacaggtg gatgaaataa tgacgaattt aaaagcagtt
     1081 attcctgtag cgggtcttgg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag
     1141 atgctaccga tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgtggctgca
     1201 gggatcaaag aaatcctcct ggtaactcac gcgtccaaga acgcggtcga aaaccacttc
     1261 gacacctctt atgaattaga atctctcctt gagcagcgcg taaagcgtca actactggcg
     1321 gaagtacagt ccatttgccc gccgggcgtg accattatga acgtgcgtca gggcgaaccc
     1381 ttaggtttag gccactccat tttgtgtgca cgacccgcca ttggtgacaa cccatttgtc
     1441 gtggtattgc cagacgttgt gatcgacgac gccagcgccg acccgctgcg ctacaacctt
     1501 gctgccatga ttgcgcgttt caatgaaacg ggccgtagcc aggtgctggc aaaacgtatg
     1561 ccgggtgacc tctctgaata ctccgttatt cagaccaaag aaccactgga tcgtgaaggt
     1621 aaagtcagcc gcattgttga atttatcgaa aaaccggatc agccgcagac gctggactca
     1681 gacatcatgg ccgttggtcg ctatgtgctt tctgcagata tttggccgga actagaacgc
     1741 actcagcctg gtgcatgggg gcgtattcag ctgactgatg ccatcgctga actggcgaaa
     1801 aaacagtccg ttgatgccat gctgatgaca ggtgacagct acgactgcgg taaaaaaatg
     1861 ggttatatgc aggcatttgt gaagtatgga ctacgcaacc tgaaagaagg ggcgaagttc
     1921 cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat ctgaccggat gtaacggttg
     1981 ataagaaaat tataacggca gtgaagattc gtggcgaaag taatttgttg cgaatattcc
     2041 tgccgttgtt ttatataaac aatcagaata acaacgagtt agcaatagga ttttagtcaa
     2101 agttttccag gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt
     2161 tttcgggtta agcgcgagtg ggtaacgctc gtcacatcgt aggcatgcat gcagtgctct
     2221 ggtagctgta aagccagggg cggtagcgtg cattaatacc tctattaatc aaactgagag
     2281 ccgcttattt cacagcatgc tctgaagtaa tatggaataa attaagtgaa aatacttgtt
     2341 actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa taatactcag
     2401 gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc acttgctgat
     2461 gtttctgact ctgaacgcta tgtttttgaa catgtggata tttgcgatgc tgctgcaatg
     2521 gcgcggattt ttgctcagca tcagccggat gcagtgatgc acctggctgc tgaaagccat
     2581 gtggatcgtt caattacagg ccctgcggca tttattgaaa ccaatattgt tggtacttat
     2641 gtccttttgg aagcggctcg caattactgg tctgctcttg atggtgacaa gaaaaatagc
     2701 ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca tcctgacgaa
     2761 gtaaataata cagaagaatt acccttattt actgagacga cagcttacgc gccaagcagt
     2821 ccttattctg catccaaagc atctagcgat catttagtcc gcgcgtggaa acgtacctat
     2881 ggtttaccga ccattgtaac taattgctct aacaattatg gtccttatca tttcccggaa
     2941 aaattaattc cattggttat tctcaatgct ctggaaggta aaaaattacc tatttatggc
     3001 aaaggggatc aaattcgtga ctggctgtat gttgaagatc atgcgcgtgc gttatatacc
     3061 gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa cgaaaagaaa
     3121 aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc gaaagagaaa
     3181 tcttatcgtg agcaaatcac ttatgttgcc gatcgtccgg gacacgatcg ccgttatgcg
     3241 attgatgctg agaagattag ccgcgaattg ggatggaaac cacaggaaac gtttgagagc
     3301 gggattcgta aaacaattaa ctggtatttg aataataatg attggtgccg gcgagttcaa
     3361 gatggctctt atcaacgtga acgtttgggg gtaattagat gaaaggaatt attttagctg
     3421 gtggttctgg aacaaggctt tatccaatta caatggggat ttcaaagcaa ttattgcctg
     3481 tttatgacaa gccaatgata tattatccgt tgtcagtgct gatgttggca gggataagag
     3541 atattttaat aataactacg ccagaggatc aacatggttt caaacgtcta cttggtgatg
     3601 gttcatcgct tgggataaat ttacagtatg aagtacaaaa ttctcctgat ggccttgcgc
     3661 aagcttttat cataggagag aaatttattg ccaatgattc tgtctgctta gttctgggtg
     3721 ataatatatt ctatgggcaa ggtttttcac caaagctaaa agaggcatcc atacgggcta
     3781 aagaaaaagc tacgatattt ggttatcagg taaaagatcc tgaacggttt ggtgtagtag
     3841 aatttgacaa ccaacgaaat gttttatcaa tagaagagaa accgagcaat cccaaaagta
     3901 actatgcagt gacaggttta tatttttata ctaacgaagt agtcgaatac gctaaacttg
     3961 tgaagccctc ttctcgagga gaactcgaaa tcacatcgat taatcagatg tatctagaga
     4021 atggaaagtt ggctgtagaa atgctaggtc gtggtttcgc ttggcttgat acaggtaccc
     4081 atgaaagttt gcttcaagct tcccaatttg ttgaaacgat cgaaactcgt caaggattta
     4141 aggtagcatg tttagaggaa atagcatttg ctaatggttg gttgacaaaa aaagaattat
     4201 taattcgctc aagcttgatg tcaaaaaata gctatgggca atatttattg caattaatca
     4261 agtgaaatat cgtggcagaa ctaaaataca aaacaataaa tagcttaaaa tggagtgcat
     4321 tggaaaggat attttcccaa ggtattcaat taatattaat gcttgttttg gcaagattac
     4381 ttggacccaa ggcatttggt ttaattggaa tgcttgcaat atttatcgca attgggcagg
     4441 tatttattga tagtggtttt agttctgcct taataagaaa aaatgatcgt acacatattg
     4501 attatactac agcattttat ttcaatgtta cagtggcgat cttattttat gttgtcctat
     4561 tcttttcagc accattaatt gcaaaatttt atcatcaacc ggaacttgaa cttttatcta
     4621 aaataatagg gttgtcattg ataactaata gcttttgcgt tgtgcctcga acaattttga
     4681 ctattgccat ggattttaaa tctcaggcaa aagtatctgt tttgtcggta gtgttagggg
     4741 gggcatttgc tctatttctg gcatggaagg gctttggtgt ttggtcttta gtagctcaaa
     4801 cgttgactgt atcaatgtgt aatgctctat tattaaatat tataaccaaa tggatacctg
     4861 gattgttttt ctcaaaaaaa tcatttctat atctttttgg gtttggtggg aaattattaa
     4921 tatcaggtct acttgataca atatataata atatgtatca aataattatt ggcaagaaat
     4981 ttagtgccgc aaacgtaggg cagtatactc aagcgaatca gatatccagt attcctgcaa
     5041 tgacactgac gaatataata cagagggtaa cgtacccaat gttcagtcat atgcaagacg
     5101 atgaagataa attgaaaaaa aattatttgt tgacactgaa attggcggca ttattcattt
     5161 ttcctattat ggccggtatt gggattatcg cacagccact cttttttatt attttagggc
     5221 ctcaatggat tgatgcatct aggctgatta taatactatc ttttgcttat atgatttatc
     5281 ccattcatgc tgtgaattta aatattttac aagtaaaagg tagaagcgac ttattcctcc
     5341 aacttgagat aatgaagaag ttaataggtg tcattgttat tttgataaca atgaattatg
     5401 ggatttatgg catgtgcttt gggttagtat ttcattccta tctttgcctg attgttaata
     5461 cgtactatac aaaaaaaatg acaaaactaa ctcttaagca gcaaatgatt gaactcggtc
     5521 cagtttggat tataactatt atatcttgtg caattgcata tattggagca ttgcatataa
     5581 gtgatgatgc actattacaa gtgctattga tggtcgtaat agcagtggta ttatatattg
     5641 gattaatatt tctactgcaa agaaatttat tgaaattagc cttatcaatt agaaaataga
     5701 gtatatttaa atgaaaaaaa tcccagtaac acaacctttt ttaccggatt taaatgaatt
     5761 tatcccgtat cttgaaaaga tttgggaaaa taaatggttg acaaataatg gtccgttcca
     5821 tcaacaactc gaaaaagaac tgtgcaagta tttaggagtt gaatacgttt ctatatttaa
     5881 taatgcaacg attgcactga ttacagcatt acaagctttg agaattacag gtgaagttat
     5941 aacaacacca tattcttttg ttgcaacaag tcactctatt ttatggaatg gattaaaacc
     6001 gatttttgtg gatattgatc ctgaaacatt caatattgat cctaaaaaaa ttgaagaggc
     6061 aataacaccg gaaacaacag ccatcatgcc cgtgcattgc tattctaatc cttgtgaagt
     6121 agaggctatc caggagattg cagataatta cggcttaaga gttatttatg acgctgcgca
     6181 tgcatttgga gtaaattata aaggtcggag cttattaagc tacggtgata tgtcggtctt
     6241 gagttttcat gcaacaaaag tatttaatac ttttgaaggt ggtgcgatta tctgtcagga
     6301 tgcaaaacta aagcagcgta ttgataggtt aaagaatttc gggatagcag atgagttaac
     6361 tgttacagca ccgggcatta atgggaaaat gagtgagcta aatgctgcat ttggtttagt
     6421 tcagttgaaa catattgata cagcaattca gcaaagacaa aaaattgatc atatgtatcg
     6481 tgaggaactt tcgtctgtaa acggtattcg tatgattgag tatgtttcag ccgctaatag
     6541 taatttttca tattttccaa ttctcataga aaaaaattat cgtatgtcaa gagatgaact
     6601 ttatacggag ttgaaaaaaa atggtatttt atctaggcgc tatttttatc cccttattag
     6661 caatatgtcg atgtatcgag gtatagagac agcgacaaag gaacatttac ctaacgctaa
     6721 tgaaattgca gataaagtat tatgcttacc gatctttaat gaactaacgg acaatgattt
     6781 cagcaggatt ataaaaacaa taagaggtta acatggcatt tttgaaagaa gaagacgttg
     6841 aatgtttagg tttcggctct gttggataca atgttctaat ttccccaaag gcatcttttt
     6901 acggagcaga aaatattcat attggtaata atgtgagaat tgatgatttc tgtgtttttt
     6961 cagcaggaaa aggtggtatt tatattggaa attatattca tattgcggtg tattcttcct
     7021 tgattggttc tgaaaaaatt gtgctaagcg attactccaa tatttcatca agggtttcga
     7081 tttattctag caatgatgat tattccggcc gttttatgag taacccaatg attcctgctg
     7141 aatttacgaa tgtcactcat gctcctgtaa taataggtaa gcacgtaatt ataggaagtg
     7201 gaagtatcgt tttacccgga gtttcaataa atgatggtgc agtaattgga gcattatcgt
     7261 ttgtaaaacg tgactgtgat gagtttactt tatatgctgg caatccatta aaaagaatca
     7321 aagaaagaca aagaaacatt gaaagattag aattggattt taggggcaaa tttgaatagt
     7381 ctcttgtttt ggctaattta aatctagggt gtttggaatg aatagtaagg tggtaatttc
     7441 aatctgttgc ataacgttta atcatgctga ttatatagag aagtgtattg aagggtttct
     7501 ggctcagaac tttccccata agatggaaat aattattgga gatgatttaa gttctgatgg
     7561 cactagagag aaattgcaga aaatacaatt acagcatcca gatttaatta aggttattta
     7621 taatcaagag aacatggggg ctaataaaaa tatattatca gttctacgtc aggccaaagg
     7681 agagtttatc gcgttttgtg aaggcgatga ttactggata catgaggata aattatattt
     7741 gcaatataaa ttgctaaaaa caaatcctag tgtagacttt tgttttcaca gtgcatacat
     7801 ggattcagag ggagaaaaga aattacattt taaatatgga gaaagaacaa aaaagtttta
     7861 tattaatgat gttttaaaag tggcaggtca atttgcgcca acatcttcat acatgttcag
     7921 gcgaaatata atatacgcac ttccagcgtg gtttccacaa agttgtattg gtgatttatt
     7981 tatggaaatt tactctatga aaaaaggagg gatttatata cccttaccta tgtctgtata
     8041 tagagtgaat tcggttggtt cttggagtga aacaataaaa agtaatttaa atgtttttac
     8101 tgaaaagcat ttgaatattg ccacgcactt gcgtttggca aaacaagaat ttaacggata
     8161 tgaaaaatat ttcaatttaa aaattgctaa tgtatacttg aatatgtgta caaggtttat
     8221 tatagaaaaa gaatataaat tatttaaagt ttatcttgaa aaagccaatg gtataaatga
     8281 acacttcacc agaaaacagc tgttatataa tggtttgcgc ttctttccga ctattattta
     8341 ctatatacat tatttaaaag caattataaa gaataagtga tgcgcttatg tattggatat
     8401 attgtttatt tttactattt attgtctctc ttttaaataa agggcgttat tcgacactgg
     8461 catataaagc atcgctttta attatattta tattcgctac aggtaattat tacaatggtg
     8521 ttgactggat aaactatcaa aaacattatg agtttattag taattcaggc atttcgagtg
     8581 aatattttgc atatgaaccc ggttttttac tcttaatgta catatttggt aatgttttag
     8641 atataaaaaa cttccatgta attgttattt ttatttcatc aatttccatt ctgtcgttgt
     8701 caaagtttat aaaacaaata cccaaaggag tcaataaaag cttaatattg ttcatgttgg
     8761 ttgcatttat ctatccatta tataatgatg ccattagaca actatttgca ttttgcatta
     8821 tacttccact tcttccaaat attaaatcaa ttgcaataaa gaaaataata ataacatgtg
     8881 ggattgctgc cctgtttcat tcttcaatta ttttaatggt gcctttattt attctaatga
     8941 gaagagaact gactcagaag tttatgtttt attctttcat ttgtgcattg ctctttttat
     9001 cattactaat aaatattgaa ttcatcgtaa atacattaaa caatttaata ccaaaaatgt
     9061 tatatgttaa attggttaac tatgtaaata atatgggtga gttcaaagtt ggtttttttg
     9121 cagttataga ttttcttggg atatgtatat tttgtgcaac ctatagagta ttcaaaagca
     9181 ataatgacat ttcagtatat gcaattggaa cgttttactt ttttatactc catctggttt
     9241 tttacggggc tccgttctta cagcggctat tatattatat ttttccatta ttatctgtat
     9301 attcatgttt tatatttagc cattgtcaga gagggagcgg ggcgcggatt cttttaccgt
     9361 taacactaat aatgacatta tctgtgtttg caagaaatat atttaatcca tactacgcta
     9421 cggactttta tgaacctaag tttttttata ccgatggtgt tttcgagaag catcttgatg
     9481 ttgaacgact gaaatataga aaatgttcgg tcattgatga atacgatcct aacttttgtc
     9541 ctaaataaaa atgaatattt tgatattaag cgatgaatat tatccttatc acatcggtgg
     9601 tgctggtgtt gttgtttcac aaattgaaaa aatactttta tctaaaggag aaaaatacca
     9661 aatcatatgc agcaaaccta aaaataaatt actatgtgaa atttataaac ttacatggcc
     9721 attatggtat ttttatgctt tagtgttgat tacgttcaaa agatatgacc gtattattgt
     9781 taacgatgtt cgaagtgcat atattttagg actgttcgga agaatagcaa cattaaaaaa
     9841 atgtgtttat ttggttcatg gaacggaagt tgatattgta tttaaatcac gtagtctaaa
     9901 aaattccttt attttatttc ctttttttta taagcggttt ataaaaaaaa taaaaaagat
     9961 tgtctttgtt agtaaatttg tcgcatcacg tactaataag gaactattaa ggaacaatat
    10021 aacaccaaac aaacaatgtg tatgttatgc aggccttggg cctgaaatct atcatttagc
    10081 agctacagat gatttatatt tagaagacga gaatattgat caaatacggc ttgtatcttt
    10141 ttctagactt gaaaaaagaa aaggctatat ggagatgata gaaattttta atgcactcat
    10201 aaaaaaagga cattctattc actgggatat ctatggtgat ggaagtttaa aatatgacat
    10261 tttaaaaaaa ataaaacaac tagagttgga caatgatatt attcttttgg ggaaaattga
    10321 cagaaataat ctatataagt atataaaccc caataaatat gatgcttact ggttgctacc
    10381 aaatgagcct gaagcatttg gtctaactta cattgaaagt tctgctatgg gtttgccagt
    10441 gtttggacct aagaaatatg gcattgagga agctattaaa gaaaatgaaa atggcttttt
    10501 ttttgaaggt aataataaat ttattcaaga cttaaataaa ataaaagcaa acaaatgtaa
    10561 gtatattcaa tctagcaaac aatgggcatt gtgttttgat gttgaaaaat ttgttaatga
    10621 attagtttct taaggggtaa tgatgaaaat tgtatatgtt ataactggtt tatctatggg
    10681 aggagctgaa aaccaagttt gtctcttggc tgatcaatta actaagttag gccatgatgt
    10741 taccttgatc tcattagata acactctaga agtaaagcct attaacgata aggtaaaact
    10801 acggcagtta tcgataaaaa agaatccaat atcattaatt attaattact ttaaattaag
    10861 aaaaataata aaaaaaattt ctcccgatgt tgtgcacagt catctttttc atgcaaatat
    10921 aatttgcaga ttgcttaagc taacaatgaa aataaagaaa ttaatttctt catcccacag
    10981 tagatatgag gggggagtta taagaatgct tatttataga gttacccaga acttggccga
    11041 tattgtaaca aatgttagcc tagatgcgtc caatgaactt cttaaacgta gagttgttac
    11101 agaaaataaa ttagtaacag tgttcaatgg tattgactgt aataaattta aatttattcc
    11161 cgatagccgt caggacgtgt tcaatgaatt ttctttcagt cgtgatataa aattgctctt
    11221 agctgttggc cgttttactg aagctaaaga ctataagaca ttattaaatg cctttaaaat
    11281 tttaataagt aaagatgata aatttagatt attggttgcc ggcaatggtg aattatttga
    11341 cagtatatca actcttaaat cgcaattaaa tttagacagt tttatttatt ttttgggtgt
    11401 cagagatgat atacccagat taatgagcgc cgcagatatc ttcgttatgt cctctgcatg
    11461 ggaaggatta ccattagtaa ttggggaggc catgtcatgc aactgtctga ttgttactac
    11521 gaattgcggc ggagtgaaag aaatattggg tgaatgtggg caaatagtac ctatccgttc
    11581 acctgattta ctggcagaaa aaatcatgtg gacgatggaa ctagatgatg aagagcgtgg
    11641 cattctcaaa aaaaatgcac aaaatagaat tcatgatcgc ttttctattg attcgatagt
    11701 tgctaaatgg cttgagttat actcctgaga tattatgaaa aaaaagataa tcgcgatcac
    11761 agctaacaca agttggtata tttataattt cagaagaaat accattctta ccttgataga
    11821 acagggatat gaagtaatag catgtgcccc attggataaa tattcatctt atatagagaa
    11881 acttggatgt gtccatgtgc cgctaataat ggatcagaat gggaaaaatc ctttaaaaga
    11941 cttattgacc cttttttgtt tttataaggt atttaagaaa tataaagttg atgttgttct
    12001 taattttacg ccaaagaata atatatatgc cacgatagtg gcaaaattat taaatattaa
    12061 agttattaac aatattgcag gattaggtac cgccttttct tcaaaaaact ttcttaattt
    12121 agttgcaata aatttatata aatatagtca aaaaaaagct gattttattt tttttcaaaa
    12181 taaagaggac gcaataatat tcaaaagttt cggaattaat gaatctaata ctgatatttt
    12241 acctggctct ggtgttgatt taagaagatt taatgtttgt cctgttcata cagataatgt
    12301 tgttcgattt gcattagttg ctcgcatgct tgtagataaa ggtgttatgc agtacattga
    12361 atgcgcagaa aatctaaaat ccaaatatgg aaaccgagtg gagtttttgt tagtgggttt
    12421 tttagatgac aataatccgc gggctgtgca ttcatctata atagaaaaat ggacaaataa
    12481 agatattgtt aaatatttag gtgtgtcgga ctctatagaa gatatcgtta gtaacgttga
    12541 ctgcgtggtt ctaccatctt tctatagaga aggtgtacca aaatccttat tagaagctgc
    12601 tgcaatggga agaccattaa taactactga tagcattggt tgtaaagaaa cagttataaa
    12661 taatgttaca ggatatttgt gtgagcctcg atctgtatct gatttatgtg ctaaaatgga
    12721 aaaaattata tctatgtcta aagaagataa agtaaaaatg gggctggcag gacgtcaata
    12781 tattgaacgt aacttcgacg aaagaattgt tgttgataag tacttaaatg ttataagtga
    12841 ttttttcgaa taattaagca gataaggtga cattgatgac tgtattggtg actggtggtg
    12901 cagggtacat tggttctcat accataatag atttattaga aaataattac caagttgtag
    12961 ccatagacaa tttatcaaat tcttcatttg aaagtataaa aagaattaaa gaagtaacca
    13021 atagacactt tgacttttat aaagtcgatc ttagaaataa aaaagagctt gatgatatct
    13081 ttctaaagca tgatatttcc tgtgtattac attttgctgg acttaaatct gtaagtgatt
    13141 caataaaaaa ccctttaaat tattatgaca ataatgtatt tggaacgctt aatcttctta
    13201 gcttaatgat tgaacattgt gtttatagat taatatttag ttcatctgca actgtatatg
    13261 gagttccgga aaaaatccct gttacagaaa attgtcaaac tggtggaact actaatccct
    13321 atggttactc taaattaatg atagagcaga taataaaaga tataacaatg tctgattcca
    13381 actggactgc aatttctctg cgttatttca atccggttgg tgctcaccct tcggggttat
    13441 tgggggagag cccaattggt atacctaata atcttgttcc ctatatcact caagttgctt
    13501 cagggaagtt aactaaatta aatgtttatg gcagtgatta taatacaaag gatggctcag
    13561 gtgttcgtga ttatattcat gtgcttgact tagcctctgg acacactgct gctttaaata
    13621 aaataggcga cttatctgga tttaatgcat tcaatcttgg aacaggaaaa ggttattcag
    13681 ttttagaact aatttcttgt tttgagaaag taaatggtgt aaaagtacct tttacaattg
    13741 ctgagcgaag agacggtgat gtcgccgaat gttggtcatc tcccaatttg gctaatgcag
    13801 tattaaaatg gaaagccaaa tactctctgg aagatatgct taaagatgct tggcgatggg
    13861 aatgtaataa tcctaatggc tattagcata tgaaattaat cagtgtaaat tattttttat
    13921 ttatgtcttg aatcgattta tataattagt tctaaaatca tgtatttcta catgatagtc
    13981 cctgacagga gtaaacaatg tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg
    14041 ggcgcaacct tgcgctcaac atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt
    14101 cccgtgaaaa gacggaagaa gtgattgccg aaaatccagg caagaaactg gttccttact
    14161 atacggtgaa ggagtttgtt gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga
    14221 aagcaggtgc aggcacggat gctgctattg attctctcaa gccatacctc gataaaggcg
    14281 acatcatcat tgatggcggt aacaccttct tccaggacac cattcgtcgt aaccgtgagc
    14341 tttctgccga aggctttaac ttcattggta ccggtgtctc cggtggtgaa gaaggcgcgc
    14401 tgaaaggtcc ttccattatg cctggtgggc agaaagaagc ctatgaactg gttgcaccga
    14461 tcctgaccaa aatcgccgca gtggctgaag acggtgagcc atgcgttacc tatataggtg
    14521 ccgatggtgc aggtcactat gtgaagatgg ttcacaacgg tatcgaatac ggcgatatgc
    14581 agctgattgc tgaagcctat tctctgctta aaggtggcct gaatctcacc aacgaagaac
    14641 tggcgcagac gtttaccgag tggaataacg gtgaactgag cagctacctg atcgacatca
    14701 ccaaagatat cttcaccaaa aaagatgaag acggtaacta cttggttgat gtgatcctgg
    14761 atgaagcagc aaacaaaggt accggtaaat ggaccagcca gagcgcgctg gatctcggcg
    14821 aaccgctgtc gctgattacc gagtctgtgt ttgcacgtta tatctcgtct ctgaaagatc
    14881 agcgcgttgc cgcgtctaaa gttctctctg gtccgcaagc gcagccagca ggcgacaagg
    14941 ctgagttcat cgaaaaagtt cgccgtgcgt tgtatctggg caaaatcgtt tcttacgccc
    15001 agggcttctc tcagctgcgt gcggcgtctg aagagtacaa ctgggatctg aactacggcg
    15061 aaatcgcgaa gattttccgt gctggctgca tcatccgtgc gcagttcctg cagaaaatca
    15121 ccgatgctta tgccgaaaat ccgcagatcg ctaacctgct gctggctcct tacttcaagc
    15181 aaattgccga tgactaccag caggcgctgc gcgatgtcgt tgcttatgca gtacagaacg
    15241 gtatcccggt tccgaccttc gccgctgcgg ttgcctatta tgacagctac cgtgccgctg
    15301 ttctgcctgc gaacctgatc caggcacagc gtgactattt cggtgcgcat acttataagc
    15361 gcattgataa agaaggtgtg ttccataccg aatggctgga ttaatctgat ttaaatcaat
    15421 taaccaacac aaggcccgga gaaaccctcc gggcttttta ttatacaaag cggcaggtta
    15481 tggccttttt ttataattta tgattaaaaa cgcgatataa tacagagccg cacagcagga
    15541 tcgctgtctt gacagttcaa ccgcatcagc gttaaaaatc ccgcagtaga tgaagttgtg
    15601 gtggtggact aatgaccact ctaaatgttt aaccggaaga agtcagagct aatgaaaata
    15661 acaatttcag gaacaggtta tgttggtctt tcaaatggta ttctgattgc gcaaaaccac
    15721 gaagtggttg cactggatat cgttcaggcc aaagtggaca tgcttaacaa gaagcagtca
    15781 ccgattgttg ataaggagat tgaagagtat ctggcgacta aagatctcaa tttccgcgct
    15841 acgacagata agtatgacgc gtataaaaat gccgattacg ttattattgc cacgcctact
    15901 gattatgacc cgagaacaaa ttattttaat acctcaagcg tggaaacggt catccgcgat
    15961 gtgacagaaa ttaatcccga cgcagtaatg attataaaat caactatccc tgtcggtttt
    16021 acagagtcca taaaagaacg gtttggtatt gaaaatgtga tcttttcgcc ggagttttta
    16081 cgtgaaggga aagcacttta tgataactta cacccatcac gcattgtgat tggcgagcaa
    16141 tccgatcgcg ctcaacgttt tgcagcgtta ttacaggaag gtgccattaa gcaagatata
    16201 ccaacgttgt ttactgactc aaccgaagct gaggcgatta aactttttgc taatacttat
    16261 ctggcgatgc gtgtggctta tttcaatgaa ctagatagtt acgctgaaag tctggggctt
    16321 aattcccgcc aaattattga gggtgtatgc cttgactcgc gtatcggtaa tcactacaac
    16381 aatccgtcat ttggttatgg tggttattgt ctgccgaaag ataccaagca gttactggca
    16441 aactatcagt cagtaccgaa taacctgatc tcggcaattg ttgacgccaa ccgcacgcgc
    16501 aaagatttta ttgccgattc tatccttgca cgtaaaccga aagttgttgg cgtctatcgt
    16561 ctgattatga agagtggttc agacaatttt cgtgcttcct cgattcaggg tattatgaag
    16621 cgaatcaagg cgaaaggcgt gcctgtaatc gtttatgagc cagcgatgaa agaggacgat
    16681 tttttccggt cgcgcgtggt acgtgatctg gatgcgttca aacaagaagc tgatgttatt
    16741 atttctaacc gtatgtctgc cgatctggct gatgtagcag ataaggttta tacgcgcgac
    16801 ttgtttggca atgattaatt attttgtttc atactaataa aaggccctaa taaattaggg
    16861 ccttttatta tggttttata aaatcaaact ttatagaagt tacgatacca ttctacaaag
    16921 ttctttaccc cctctttaac tgacgtttca ggtttgaatc ctattacgtc atacagtgct
    16981 tttgtatcag cactggtttc cagtacatca ccgggttgga gaggcatcat attcttgttg
    17041 gcttcaatac ccagggcctc ttctaaggca ttgatatagt ccatcaactc cacaggcgaa
    17101 ctattaccaa tgttatagac acgatatggt gctgaacttg ttgctggcga gcctgtttct
    17161 acagcccagt gtggatcttt ttctggaata acatcctgta agcgaataat agcttcggca
    17221 atatcatcaa tataagtaaa gtcacgcttc attttgccga agttgtaaac atcaatgctt
    17281 ttaccttcca gcatggcttt agtgaattta aataatgcca tatccggacg tccccatgga
    17341 ccataaaccg taaagaaacg cagccctgtg gtcggtaagc tatacaaatg agaatacgta
    17401 tgagccatga gttcattcgc ttttttagtt gctgcataca gcgaaacagg atgatctaca
    17461 gagtcatctg tagagaaagg catcttgcgg ttcatgccat aaacagaact ggaggaagcg
    17521 taaagtagat gctgaacatt attatggcga catccttcca gtatgttcag gaatccaatc
    17581 aggtttgcat ctgcatatgc attgggattt tcaagggagt aacgtacacc ggcttgcgca
    17641 gcgaggttta ttacgcgttc gaaccgctcg tctgcaaaca gtgccgccat tttctcacga
    17701 tcggccaggt caattttata aaaactgaag ttgtcgtgct tgagtaaatc aagtcgtgct
    17761 tgtttgaggt tgacatcgta ataatcattt aagttgtcaa tgcctacaac ctgatgacca
    17821 gctgcaagaa gccgtttact tacatagaaa ccgataaagc cagcagctcc cgtaaccaga
    17881 aatttcattt ataatcctcg ctcaggctag aatatagcca atcttcatct ggcataactg
    17941 aaagttaaat cataccgtta gacaagaaaa aaagataatc ggtatcagtt ctaaacctgg
    18001 ctgttttttc tggtaacgtg ctcattttac aatcaaagct gttctaagct gactatacaa
    18061 gccgacgtca ttatttccaa ccgtatggca gaagagctta aggatgtggc agacaaagtc
    18121 tacacccgcg atctctttgg cagtgactaa catcctgtta tcagggcgat tttcgccctg
    18181 attctcttat gtttcctttg taataattca ttatttttat catttatcct atagcattca
    18241 tggcgattat cgctaaacta tggcgactta aaattatttc gtccgttagg gtaatgatga
    18301 gagtagaaaa taataatgtt tctgggcaaa accatgaccc ggaacagatt gatttgattg
    18361 atttactggt gcagttgtgg cgcggcaaga tgacaattat catctctgtc attgttgcta
    18421 ttgccctggc tatagggtat ctggccgttg ccaaagaaaa gtggacttca acagctatcg
    18481 ttactcaacc tgatgtgggg caaatcgctg gttataacaa cgccataaat gtgatttatg
    18541 gttcagctgc gccgaaagtg tctgagatcc agtcgatgtt aattggtcgc ttcagcacga
    18601 cattttctgc attagcagaa acgctggata atcaggaaga acctgaaaaa ctgaccattg
    18661 agcctaccgt taaaaatcag tcattacctt tagcggtttc ttatgtgggt gaaacacctg
    18721 aaggtgcgca gaagcagctg gcgcagtata tccagcaagt tgacgaccag gtaaatgaag
    18781 aactggaaca agacctgaaa gacaacatcg cgctgcaaat gaaaaacttg caggattctc
    18841 tgaagacgca ggaagtggtc gctcaagaac agaaagatct gcgtatccgt cagattcagg
    18901 aagcgttgca gtatgcgaat caggcgcagg tgacaaagcc acagattcag cagacacagg
    18961 acgtaaccca ggatactatg ttccttttgg ggagcgaagc gctggagtcg atgattaagc
    19021 acgaggcgac tcgtccgttg gtgttctcat caaactacta tcagactcgt caaaacctgc
    19081 tggatatcga caatcttgac gttgataaac ttgatattca tgcttaccgc tatgtaatga
    19141 aaccgacatt acctattcgt cgcgatagtc cgaaaaaggc aatcaccttg attctggcgg
    19201 tgctgctggg cggcatggtt ggcgcgggga ttgtgctggg gcgtaatgct ctacgcaact
    19261 acaacgcgaa gtaatctttt tgggtttaaa gaaaacgggc agggtggtga caccttgccc
    19321 gtttttttgc cggatacgac aacaacatcg catccgctta ccccgcaact cactgatgcc
    19381 gtttgcgcag gttctcaatc accgtcgtta aatccagatc ctgatcctgc aacaacacca
    19441 gcaggtgata catcaagtca gatgcttcgt tggtgagctc aaagcggtca tgcaccgttg
    19501 ctgccagtgc ggtttccacg ccttcttcac ccactttctg cgcaatgcgt ttggtgccgc
    19561 tggcatacag tttggcagtg taagaggttt ccgggtcggc agatttgcgt tcggcgagca
    19621 gttgttccag ttgatacagg aacagccact ggtgagcggt gtcgccgaag cagctgctgg
    19681 tgcctttgtg gcaggtcggg ccgatgggat tcgccagcaa cagtaacgtg tcgttgtcgc
    19741 agtccggcgt aatatttact acgttgagga aattgcccga cgtttcgcct ttggtccaca
    19801 gccgttgttt agtgcgcgag aagaaggtga ctttgccgct ttcgatggtt ttgtctaagg
    19861 cttccgggtt catgtagccc agcattaaca cttcgccgga taccgcgtgt tgcacaatca
    19921 ccggcatcag tccgtcggtt ttttcccagt ccagttcgcg acgttgttgt tctgttaaca
    19981 tatcctgatc tccacgccct gtgttgccag gtacgctttt aattcaccaa tattgattat
    20041 ttgtttgtgg aatacggaag ctgccagcgc gccgtcaacg tcggcatcgc ggaaggcttc
    20101 gaggaagtgt tccatggtgc ccgcgccacc ggaggcaatc agcgggacgt ggcaaacttc
    20161 acgtactttt ttcagttgtt cgaggtcgta accgttacgc acgccgtcct gattcatcat
    20221 gttgaggacg atttctccgg caccgcgttt ttgtacttcc tgtacccagt cgagcgtttc
    20281 ccactgagtg acgcggg
//
