LOCUS       HM204927               13346 bp    DNA     linear   BCT 01-JUN-2011
DEFINITION  Escherichia coli serogroup O118 O-antigen gene cluster, complete
            sequence.
ACCESSION   HM204927
VERSION     HM204927.1  GI:312162714
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Liu,B., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Feng,L., Shashkov,A.S., Wang,L. and Knirel,Y.A.
  TITLE     Structural and genetic relationships between the O-antigens of
            Escherichia coli O118 and O151
  JOURNAL   FEMS Immunol. Med. Microbiol. 60 (3), 199-207 (2010)
   PUBMED   21039922
REFERENCE   2  (bases 1 to 13346)
  AUTHORS   Liu,B., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Feng,L., Shashkov,A.S., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin,
            Tianjin 300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O118"
     gene            174..1532
                     /gene="rib"
     CDS             174..1532
                     /gene="rib"
                     /note="bifunctional ribulose 5-phosphate
                     reductase/CDP-ribitol pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="Rib"
                     /protein_id="ADQ37332.1"
                     /db_xref="GI:312162715"
                     /translation="MKNIAVILASGTGSRFGAKIPKQFVKLAGKPVIQYTLHAFQNAK
                     KIDEIIVVTNEEYVDFVSALTMEHNYFKVTKIIHGGKERYESSWAALQSIELDECNVI
                     FHDAVRPFVSQKIIDDCIDGLNNYNAIDVVVDPTDTIVKIKNKEIMSIPDRRFLARGQ
                     TPQGFKKSLIEKAYIDFLKEEKKVASDDCGIFLKYMPNEPIGIIKGDESNFKITHQQD
                     IYLADNLIKDGLLELISENNDSIARTISEKVIVIFGGNSGIGKTLLVKAKSLDGNVYS
                     FSRSNGIDISNAMNVRKALQSVFEKEGRIDYIINTAAVLIKKPINFMSDEDIEQSCNT
                     NYLGAINIAREGYGYLKKTKGMLINFTSSSFTRGRASYSLYSSSKAAVVNLTQALSEE
                     WLNLGIKVNCINPERTDTPMRRVNFGIEPPESLLTSEQVADMTLSIMSSDYTGQVVAV
                     KK"
     misc_feature    174..13192
                     /note="O-antigen gene cluster"
     gene            1537..2757
                     /gene="wzx"
     CDS             1537..2757
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADQ37333.1"
                     /db_xref="GI:312162716"
                     /translation="MSLRKNISGLAIAQIFSYIAPLLQFPYLSRIVGDSYLGAIIFIL
                     SISQMAMVLTDYGFDLSIGEQIARGKRNNKWLSVFYCQTGVIKSFLLVVAFVFIISVI
                     RLSKSDIPEQLIIGLMICVTLNAYNPYWIFQGLEKIYIYSALVIISRLIGLVLIYLLI
                     RTPDDYVYYSWILAFQAGMTTIISHIIISRWGVRVCRIKVKGIIKQFKYSFDFFLSRA
                     CVSLYSSGCSVFLATFGGSLNQVAIYGVAEQLYKAGVQVFSPVISALTPYMVRTKNYI
                     IFYKIIVVCITVTVIGMCVGFLFGAQIITLIYGNGYQESKSVLDVFMVVILASVFGML
                     LGYPALLPLGLGKVANYSVIASGILQIIMFCILYIGLFPITAISVCLTYLICDWFMFF
                     IRLYNFMKYRKING"
     gene            2750..3568
                     /gene="wdbI"
     CDS             2750..3568
                     /gene="wbdI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdI"
                     /protein_id="ADQ37334.1"
                     /db_xref="GI:312162717"
                     /translation="MDNQLRAAQMRMLAILNEVDRICRLNKINYWLDAGTLLGAKRHG
                     GFIPWDDDIDICMLRDDYNKFIMACDKQLNKEIFFLQTAYSDEYYVDFNTPCKIRMNN
                     TEIIEKSEVKYKYYDERSHHGLFLDIFPYDKYSSNKYIRKYVERFISQLCKLKVLSKY
                     SGFPITKKILIKCLSSIICKRRLLAFVDLVSFRMSERKHDYLLGAGIETPFHRAYFSE
                     DTIFPLSEIKFEGRMNLCPNNVDKYLCMMFGDNYMTIPPAAQRVWHYHSINIGK"
     gene            3575..4675
                     /gene="wzy"
     CDS             3575..4675
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADQ37335.1"
                     /db_xref="GI:312162718"
                     /translation="MVEGFVIYNSIVLLALLFCYLKRFFLPRYFTVCLFLVLWVPLAL
                     RYGIGRDYFSYIEIYKNVLVARDGIELGFYYINYILAYFNFHYQYLFALSSFITVYFC
                     VKSFDRDYTLVSIILFCALIYLQAFSIIRQMIAVSLCLYSCSLWNKGCKCKSSVFLIL
                     APLFHFSAIIILLLAIASRYIKINTTRCYFVFVFALVFIFILNGVDFIFSNAILLDSK
                     YGYYVTSAFNRPTEIGSGIGVAIALFLPFMVFIKSTQLYEYNKNYNFMLLINLVYVIS
                     YTLSLKIYIFARFTDVLSFVQILLIPVILKISSPKGIYYNLMMAVILLHILLFEANLN
                     ANTISAGNISNSGLGIMPYKSILNTKIVGIYD"
     gene            4668..5777
                     /gene="wdbJ"
     CDS             4668..5777
                     /gene="wdbJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdbJ"
                     /protein_id="ADQ37336.1"
                     /db_xref="GI:312162719"
                     /translation="MTSKILFITPLPPPVTGQSLACIELYNYLDKDNNCIIDVINLSK
                     QSFISGKGSFKRVAEIAGVLLRLLFLRRDYDLIYFTPAESKMGSYKDQLIYLFMFAKL
                     KKMYIHLHGGAGMKKLLEKDKSLIFKLNKYFISRLAGVIVLGERLKSIYNGIIESDKI
                     YAVENFSQDYLFIRQSELKLKFNNRNKIKILFLSNLLPGKGYIELLKSADFLDDSCVE
                     FHFAGGFEDEQAKKIFLEAINLKNNIFYHGIVSGDEKRNLLIKSDIFCLPTYYPYEGQ
                     PISILEAYAAGCVVLTTNHSGIFDVFEPWRNGIEVDKKSTSDLVNAINYFNSNREEIR
                     KIAINNIVDARKKYRLKHHLEKMMTVLMNHEVIQQ"
     gene            5774..6910
                     /gene="wbuX"
     CDS             5774..6910
                     /gene="wbuX"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuX"
                     /protein_id="ADQ37337.1"
                     /db_xref="GI:312162720"
                     /translation="MKKYKICNRCVMDTSDPNISFDDNGQCDYCSNFDKNILPNWNAN
                     NSGLSKLLEMSESIKKDSKGSDFDCIIGLSGGLDSSYAAYIAKEKMGLRPLLFHVDAG
                     WNTDQAVGNIEKLIEGLGLDLYTEVINWEEMKDLQLSFLKSGIPDQDLVQDAAFFSSL
                     YKFARKHKIKHVITGSNFSTECCREPEEWGGYLGIDTMLFNDIHRQFGTRPLNSFPLV
                     DILIYKVFYQKILGMKVHHPLNLVPFTKKDAENELSEKFGWQPFQHKHHESRFTRFYE
                     DYWLPRRFGYEKRRAHFSSLIMTGQMTREQALERLLKPEMDEHFLEQEFEYIAHKLGI
                     SVDDLKELFSIPKKTYKDYKNKRWLISLGTNILRTLGLEKRYFR"
     gene            6907..7533
                     /gene="wbuY"
     CDS             6907..7533
                     /gene="wbuY"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuY"
                     /protein_id="ADQ37338.1"
                     /db_xref="GI:312162721"
                     /translation="MIKIIDYGIGNIQAFLNVYKRLGINSGIAHSIDDIKSATHLILP
                     GVGAFDQAMTLFNNSGLRESIEKRIFEDRVPIIGICVGMQMLATSSEEGSLPGLGWIP
                     GTVRAFSSNISSRSLPMPHMGWNSLIKKVNTPLLKDFKEESSFYFLHSFYYECDDAGD
                     VLATAEYGHNFHCVIGRNNIYGIQCHPEKSHSSGYQLLKNFAEIQYNA"
     gene            7526..8314
                     /gene="wbuZ"
     CDS             7526..8314
                     /gene="wbuZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuZ"
                     /protein_id="ADQ37339.1"
                     /db_xref="GI:312162722"
                     /translation="MLRPRIIPCLLVHDSGLVKTVNFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSRGLEPNYALIKKLAAECRMPLCYGGGVTSVAQAVKIISLGVEKVSI
                     SSAAVENPNIVKDLAEAVGKQSVVVVLDVIKRKGLFSKGYELSTRNNTRTYKIDPISF
                     AQKMVALGAGEIVINFVDNDGVMGGYDVEYSSTIKSQINVPVTFLGGAGNYEHLSALI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTPEQKDIICNNLKKNY"
     gene            8324..9361
                     /gene="fnlA"
     CDS             8324..9361
                     /gene="fnlA"
                     /note="4,6-dehydratase, 3- and 5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ADQ37340.1"
                     /db_xref="GI:312162723"
                     /translation="MMFKDKILLITGGTGSFGNAVLNRFLSTDIKEIRIFSRDEKKQD
                     DMRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNI
                     LGTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDRNKTVI
                     CGTRYGNVMASRGSVIPLFVDLIRAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGN
                     NGDIFVQKAPAATIDTLAIALKELLNVPDHPVNVIGTRHGEKLYEALLSREEMIAAID
                     MGDYYRVPPDLRDLNYGKYVEQGDSRISEIEDYNSHNTQRLDVEGMKELLLKLAFIRA
                     IRAGEKYNLDS"
     gene            9363..10466
                     /gene="fnlB"
     CDS             9363..10466
                     /gene="fnlB"
                     /note="reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="ADQ37341.1"
                     /db_xref="GI:312162724"
                     /translation="MRILVTGANGFIGRNLCLRLEELGYKDLIRIDRESTKQDLEQGL
                     QDADFIYHLAGINRPKTDDEFISGNSDLTKHIVEYLLSIGKNTPIMLSSSIQAELNNA
                     YGVSKAVAESYVEKYAAASGSSYYIFRYPNVFGKWCKPNYNSFIATFCYNISNDIEIT
                     INDASAPVNLVYIDDVCTDAIALLSGTVESGYKVVAPIYSTTVGEVAELIYSFKNSRS
                     TLITEAVGTGFTRALYSTWLSYLPAEKFAYKVPFYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELKVSSGEFKIVETVPGWT
                     HDITNIGTEELIVMLWANEIFNRDEPDTIARPL"
     gene            10466..11596
                     /gene="fnlC"
     CDS             10466..11596
                     /gene="fnlC"
                     /note="C-2 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="ADQ37342.1"
                     /db_xref="GI:312162725"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHVIVHTGQNYDYE
                     LNEVFFNDLGVRKPDYYLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMYEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            11596..12807
                     /gene="wbuB"
     CDS             11596..12807
                     /gene="wbuB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuB"
                     /protein_id="ADQ37343.1"
                     /db_xref="GI:312162726"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRAVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGHLVKKIKARCQCPAYLILRDMFPQWVIDAGMLNAGSPIERYFRLFEKI
                     SYRQANRIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAVYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGLGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            12794..13192
                     /gene="wbuC"
     CDS             12794..13192
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ADQ37344.1"
                     /db_xref="GI:312162727"
                     /translation="MRLIDNDQLDKLYDQAGQSERLRSHLMMHGSHQEKVQRLLIALV
                     KGSYVEPHYHALPHQWEMFIVMEGQLQVCLYGRNGEVIKQFIAGDNTGISIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDSSFAKLFV"
BASE COUNT     4237 a   1996 c   2696 g   4417 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tattttttga ttatgtgcga ttaaattaaa
       61 atcgtatatc atttagagtt atatctgttc tggcactcaa ctccattttt ggggtaagat
      121 tatataggtt atttcgatat cttgataaca gacttaatag attaaggggt cttatgaaaa
      181 atattgcagt aattcttgca tcaggcactg gaagtcggtt tggggctaaa atacctaaac
      241 aattcgttaa acttgcagga aaacctgtaa ttcaatatac tctacatgcc ttccaaaatg
      301 caaagaaaat tgatgaaatt atagttgtta caaatgaaga atatgtggac tttgtgtctg
      361 ctttgactat ggaacataac tactttaagg ttacgaaaat aatccatggt ggtaaggaac
      421 gctatgaatc ttcgtgggca gcgttacaaa gtatagaatt agatgaatgt aatgtgatat
      481 ttcatgacgc cgtgcgaccg tttgtttctc aaaaaatcat cgatgactgt attgatggtc
      541 ttaataatta caatgcaatt gacgtggtcg tagatccaac cgatacgatt gtaaaaatca
      601 aaaataaaga gattatgtct attcctgaca gaaggttttt agctagaggg caaactccac
      661 aaggttttaa aaaatcatta attgaaaaag catatattga ttttttaaag gaagaaaaaa
      721 aagttgcttc tgatgattgt ggtattttcc tcaaatacat gccaaacgaa cccataggga
      781 ttattaaagg cgatgaatct aattttaaaa taacgcacca gcaagacatt tatcttgcgg
      841 ataatttaat taaggatggt ttgctggagt taatatctga gaataatgat tcaattgcgc
      901 gaacgatatc agaaaaagtc atagttatat ttggagggaa tagtggtata gggaaaactc
      961 ttttagtaaa agctaaatct cttgacggaa atgtttattc attcagtcgt agcaatggaa
     1021 ttgacatcag taatgccatg aacgtaagaa aggcattaca gtctgtattc gaaaaagaag
     1081 gtcgaattga ttatataata aatacagctg cagttttgat aaaaaaacca attaatttta
     1141 tgtcagatga ggatatagaa caaagctgta atacaaacta tttaggggca attaatatag
     1201 ctagagaagg ctatggttat ttaaagaaga caaaagggat gttaattaac tttacttcta
     1261 gctcatttac acgtggaaga gcttcatata gtctttattc atcatctaaa gcagctgtgg
     1321 tgaatttaac acaggcctta tcagaagagt ggttaaattt gggaattaaa gttaactgta
     1381 taaatccaga gcgtacagat actcctatga gaagagtcaa ttttggtatt gaaccaccag
     1441 aatccttact aacctcagaa caggttgcgg acatgacttt atcaattatg tcctctgatt
     1501 atacagggca agtagtagct gtgaaaaaat aacttcatgt cattgcgaaa aaatattagc
     1561 ggcttagcta tagctcaaat atttagctat atagcaccat tactgcaatt cccttatcta
     1621 agtaggattg taggggattc atatttaggt gctattattt ttattctctc aatatcacaa
     1681 atggcaatgg tgttgacaga ttatgggttt gatttatcga ttggagaaca gatagcaaga
     1741 gggaaaagaa ataataaatg gttaagcgtt ttttattgtc aaactggagt aattaaatca
     1801 tttctattag tggtagcatt cgtatttatt ataagcgtga ttcgcctgtc aaaaagtgat
     1861 attcctgagc aactgataat tggattaatg atttgtgtta cattaaatgc ttataatcca
     1921 tattggatat ttcagggcct tgaaaagata tatatatata gtgcattagt aatcatttca
     1981 agattaatag ggcttgtttt aatctatttg ctaattagga ctccagatga ttatgtatat
     2041 tactcttgga ttttggcatt tcaagcgggg atgacaacga taatcagcca tattattata
     2101 tccagatggg gggttcgtgt ttgtcggata aaagtaaaag gtataattaa gcaatttaaa
     2161 tatagttttg actttttttt atcaagagct tgtgtttcat tatactcatc tggttgcagt
     2221 gttttcttag caacatttgg tgggagtctg aatcaagttg cgatctatgg cgttgcagaa
     2281 cagttatata aagcgggagt acaggtattt agccccgtta tttcagcgct aactccttac
     2341 atggtaagaa caaaaaacta tattattttt tacaaaatta tagtggtatg cataactgtc
     2401 acagtgatag gtatgtgtgt cgggttttta tttggtgctc aaatcattac attgatttat
     2461 ggtaatggat atcaggaatc caaatctgta ctagatgtct ttatggttgt aattcttgca
     2521 agtgtatttg gtatgctttt aggatatcct gcattgcttc ccttaggatt gggtaaggtt
     2581 gctaattata gtgtcattgc ttctggtata ttgcaaataa taatgttctg tattttgtat
     2641 attggtttat ttcctattac agctattagt gtatgtctga catatctaat ttgtgattgg
     2701 tttatgttct ttattcgtct ttataacttt atgaaatata ggaaaattaa tggataatca
     2761 gttacgtgca gcccaaatga ggatgctggc aattcttaac gaagttgatc gaatatgcag
     2821 actgaataaa ataaattatt ggctagatgc agggaccttg ttaggtgcca aaagacatgg
     2881 tggttttatt ccttgggatg atgatataga tatttgtatg ttaagggatg attataataa
     2941 atttattatg gcatgtgaca aacaacttaa taaagagatc ttttttcttc aaacagctta
     3001 ttcagatgaa tattatgtgg actttaatac accatgtaaa ataagaatga ataatacgga
     3061 gattatagaa aagagtgaag tgaaatataa atactacgat gaaagatctc atcatggttt
     3121 gtttcttgat atattcccct atgataaata ttcatctaat aaatatatta ggaagtatgt
     3181 tgaacggttt atatctcaac tatgtaaact taaagtatta tcaaaatact ctggatttcc
     3241 tattacaaaa aaaatactta taaaatgttt aagtagtatc atttgtaaaa gacgattatt
     3301 agcttttgta gatttagtta gttttcggat gagtgagagg aaacatgatt atttactagg
     3361 cgctggaatt gaaacaccat ttcatagagc ctacttttct gaggatacaa tctttccatt
     3421 aagtgaaata aaatttgaag gaaggatgaa cttatgccca aataatgttg ataagtatct
     3481 ctgtatgatg tttggggaca attatatgac tattccacct gcagctcaga gggtgtggca
     3541 ctaccatagc attaatattg ggaaatagac tactatggtt gaagggttcg ttatttacaa
     3601 tagcatagtc ttgcttgctt tattattttg ttatttgaag agattttttt taccccgtta
     3661 ctttactgtc tgcttatttt tggttctttg ggttccatta gcgcttaggt atggtatagg
     3721 gcgtgattat tttagctata ttgaaattta taaaaatgtt ctagttgcaa gagatggtat
     3781 tgagctgggt ttttattata taaattatat acttgcttat tttaattttc actatcaata
     3841 tttatttgca ctgtcatcct tcattacagt ttatttttgt gtaaaaagct ttgatagaga
     3901 ttatacgtta gtatctatta tattattttg tgctttaatt tacttgcagg cattcagtat
     3961 tattagacaa atgattgcag tgtcactctg tctctatagt tgctcattat ggaataaagg
     4021 ttgtaaatgt aaatcatcag tattccttat attggcacct ttatttcatt tctctgcaat
     4081 aataatatta cttttagcta tagcaagccg atatataaaa ataaatacga caagatgtta
     4141 ttttgtattt gtttttgctc ttgtattcat atttatattg aatggggtag attttatatt
     4201 ttcaaacgct atattattag atagtaagta tggatactat gtaacatcag catttaatcg
     4261 acctacagaa attggctcag gaataggtgt tgctatagca ttatttctac cgttcatggt
     4321 atttataaaa tcaactcagt tatatgagta taataaaaac tataatttta tgctgcttat
     4381 aaatctagta tatgttattt cttatacact ttctctgaaa atatatatat ttgcacgatt
     4441 tacagatgtt ctatcttttg ttcagatatt attgatacca gtaatactta aaatctcttc
     4501 ccctaaaggg atttattata atttaatgat ggctgttatt ttgcttcata ttttattatt
     4561 tgaagcaaac cttaatgcga atacaatttc tgcaggaaat atttcgaata gcggattagg
     4621 catcatgcca tataaaagca tactaaatac taaaatagta ggtatatatg actagtaaaa
     4681 tattgtttat aactccattg cctccaccag ttactggaca atctttagct tgtatcgagt
     4741 tatataatta tctagataaa gataataatt gtattattga tgtaataaat ctttcaaaac
     4801 agtcctttat atcagggaaa ggctcgttca aaagagttgc tgaaatagct ggtgtgttat
     4861 tacgtttatt atttcttaga cgagattatg atttaattta ctttacacca gctgaatcta
     4921 aaatgggaag ttataaagat caattgatat atctttttat gtttgcaaag ctaaagaaaa
     4981 tgtatattca cttgcatggc ggtgcaggca tgaaaaaatt attagaaaaa gataaatcat
     5041 taatcttcaa attaaataaa tatttcatct ctaggcttgc tggagtgata gtgctgggtg
     5101 aaagattaaa gagtatttat aatggaatta ttgaaagtga taaaatttat gcagttgaga
     5161 acttttctca agattattta tttataaggc aaagtgagtt aaaattaaaa tttaataatc
     5221 ggaacaaaat aaaaatactt tttcttagta atttgttgcc aggaaaagga tatattgaat
     5281 tgttaaaatc tgcagatttt ttagatgatt cttgtgtgga attccatttc gctggtggtt
     5341 tcgaggatga acaagcaaaa aaaatttttt tagaggctat taatttaaag aacaatattt
     5401 tctatcatgg gattgtcagt ggcgatgaaa aacggaatct actaattaaa tctgatattt
     5461 tttgtttacc aacatattat ccttatgagg ggcaacctat aagcatactt gaggcatatg
     5521 ctgctgggtg tgttgttctt actaccaacc acagtggtat atttgatgtg tttgaacctt
     5581 ggcgtaatgg aatagaagtt gataaaaaaa gcacatctga tttagtaaat gcaataaatt
     5641 attttaattc gaacagagaa gagattcgta aaattgccat caataatatt gttgatgcaa
     5701 gaaaaaaata tagacttaaa catcatttag aaaagatgat gacagtatta atgaatcatg
     5761 aggtaataca gcaatgaaaa aatataagat atgcaataga tgtgttatgg atacatctga
     5821 tccaaacatt tcatttgacg ataatggcca gtgtgattat tgttcaaact ttgataaaaa
     5881 tattttacca aattggaatg ctaataattc aggcttatca aaattgcttg aaatgtcaga
     5941 atcaattaaa aaagacagta agggaagtga ttttgattgt attattggct tatcaggagg
     6001 tttagatagc tcttatgcag cgtatattgc gaaagaaaaa atgggactac gcccattatt
     6061 atttcatgtt gatgctggat ggaatactga tcaggcagtt ggtaatattg aaaagcttat
     6121 cgaaggactt ggtttagatc tttacacaga agttataaac tgggaagaaa tgaaagatct
     6181 tcaactgtct ttcttaaaat caggaattcc agatcaagat ttagttcagg atgctgcatt
     6241 tttttcatct ttatataagt ttgctcggaa acataaaatt aaacatgtta ttactggttc
     6301 aaatttttct acagagtgct gtcgcgaacc agaagaatgg ggagggtatt taggtattga
     6361 tactatgtta tttaatgata tccacagaca gtttggaacc cggccattaa attcattccc
     6421 cttagtagat atactcatct ataaggtttt ttatcagaaa atccttggaa tgaaagtaca
     6481 tcatccattg aatttagtac catttactaa gaaggatgcc gaaaatgaac ttagtgaaaa
     6541 atttggctgg caaccttttc aacataagca ccatgagtcc cgttttactc gtttctatga
     6601 agattattgg ttaccacgta ggtttggtta cgaaaaacgt agagctcatt tctcaagtct
     6661 aattatgaca gggcaaatga ctcgcgaaca agctcttgag aggcttttaa agccagaaat
     6721 ggatgaacat tttcttgaac aagagtttga gtatattgca cacaaactag gcattagtgt
     6781 tgacgattta aaagaactat ttagtattcc caaaaaaacc tacaaggatt ataaaaataa
     6841 gcgttggttg attagtttag gtacaaatat cctacgaaca cttggattgg aaaaaaggta
     6901 tttcagatga tcaaaataat tgattatggt attggaaata tacaagcatt ccttaatgta
     6961 tataagcgat taggtattaa ttcaggtatt gctcattcta tcgatgatat taaaagtgct
     7021 acacatttga tattacctgg agttggagct tttgaccaag ctatgacact ttttaataac
     7081 tcaggtttac gagaaagtat tgaaaaacgt atttttgagg atcgggttcc aattattggt
     7141 atctgtgtag gtatgcagat gcttgctacc tctagtgagg aggggagttt gcctggttta
     7201 ggatggattc ctggtacggt tcgagcattc agcagtaata tctcttcaag aagcctcccg
     7261 atgcctcata tgggatggaa tagtttaatt aaaaaagtaa atactccatt actgaaagat
     7321 tttaaagaag agtcttcctt ctattttttg cactcattct attacgaatg tgatgacgcc
     7381 ggtgatgtat tagcaacagc tgaatatggt cataattttc attgtgtcat tggcaggaat
     7441 aatatatatg gcattcaatg tcatccggag aaaagtcatt catcaggtta tcaactctta
     7501 aaaaattttg cagagatcca atataatgct tagaccaaga atcataccct gtttacttgt
     7561 ccacgattct ggcttagtca aaacagttaa tttcaaatca ccgaagtatg ttggtgatcc
     7621 aattaatgcc gttaaaattt ttaatgagaa agaagctgat gaattgatgg tccttgatat
     7681 tgatgcaact tcaaggggac ttgaacctaa ttatgctcta ataaaaaaac tcgcagcgga
     7741 atgccgaatg ccattatgct atggaggagg tgtaactagt gtagcacagg ctgtaaaaat
     7801 tatctctctt ggtgttgaaa aggtatcgat aagttcagcg gccgttgaaa atccaaatat
     7861 tgtaaaagat ttggcagaag ccgttggtaa acaaagtgtt gtagttgtgc tagatgttat
     7921 taaacgtaaa ggtctttttt caaagggtta tgagctttcg actaggaata acacgcgaac
     7981 gtataaaatc gatcctatct cgtttgctca aaaaatggtt gctttgggtg caggcgaaat
     8041 cgtcatcaat tttgtagata atgatggtgt tatgggggga tatgatgttg aatattcttc
     8101 gactatcaaa tcgcaaataa acgtacctgt tacatttctc ggtggtgcgg gaaactatga
     8161 acatctctct gcattgattg accaatgtgg catcgttggt gcagcagctg gtagtctttt
     8221 tgtatttaaa ggtaagtata gagctgtgtt gataagctac cctacacctg agcagaaaga
     8281 tattatttgc aataatttaa agaagaatta ttaataaggg gttatgatgt tcaaagataa
     8341 aatactgtta attactggtg gtacaggttc atttggtaat gctgtattaa accgttttct
     8401 ttctacagat ataaaagaaa ttcgtatttt ttctcgcgat gaaaagaaac aagatgatat
     8461 gcggaaaaaa tataataatc agaagctgaa attttatatt ggtgatgtca gagattaccg
     8521 tagtattttg aatgcgactc gcggtgttga ttttatatat catgcagcgg cacttaagca
     8581 agttccatca tgtgaatttc atcctatgga agccgttaaa actaatatcc ttggtacgga
     8641 aaatgttctt gaagcagcta tagcgaatga agtgaagagg gttgtatgcc taagtactga
     8701 taaagctgta tacccgatta acgcaatggg tatttcaaaa gctatgatgg aaaaggtcat
     8761 ggtcgcgaaa tcccgtaatg ttgatcgcaa taaaacagta atatgtggta cccgttatgg
     8821 gaatgttatg gcatctcgcg gttcagttat tccattattt gttgatctta ttagagcggg
     8881 caagccactc acaataactg atcctaatat gacccgcttt atgatgactc ttgaggatgc
     8941 ggtagattta gttctttatg cgtttgaaca tggtaataat ggtgatatct ttgtgcaaaa
     9001 agcacctgca gcaactattg acacattagc tattgcttta aaggaactac taaatgttcc
     9061 agaccatccg gtaaatgtca ttggaacgcg tcatggcgag aaattatatg aagctctact
     9121 tagtcgtgag gaaatgatcg ctgctataga tatgggcgat tattaccgtg tcccgccaga
     9181 tcttcgggac cttaattatg gaaaatatgt tgagcaaggt gatagccgaa tatctgaaat
     9241 agaagattat aactctcata atactcaacg gttagatgtt gaaggcatga aagagctctt
     9301 gctaaaatta gcctttattc gagcaattcg tgctggtgaa aaatataatc tggattcatg
     9361 atatgagaat attagttact ggtgcaaatg gttttattgg tcgtaattta tgtttgaggc
     9421 ttgaggaact tggttataaa gatcttatta gaattgatcg agaatcaacg aagcaagatc
     9481 ttgaacaagg cttacaggat gccgatttta tttatcactt agctggtatc aatagaccta
     9541 agactgatga tgagtttatt tctggaaaca gtgatttaac aaagcatata gttgagtatc
     9601 tcctttctat tggtaagaat acaccaatta tgctaagttc ttcgatacaa gctgaactta
     9661 ataatgctta tggggttagc aaagctgtag ctgaaagcta tgtcgaaaaa tatgctgctg
     9721 ctagtggttc ttcgtattat attttcagat atccaaacgt ttttggtaaa tggtgtaagc
     9781 caaactataa ttcttttata gcaacttttt gctacaatat ttccaatgat attgagatta
     9841 ctatcaatga tgcatcagcg ccagttaatc tggtgtatat tgatgatgtt tgtactgatg
     9901 ctatagctct tctctctggg acggttgaaa gcggatataa agttgttgca ccaatttatt
     9961 caacaacagt tggtgaggtt gcagaattaa tttatagctt caaaaatagc cgttccaccc
    10021 tgatcacaga agctgtcggg acgggattta cccgtgcatt gtattctaca tggctgagtt
    10081 atttaccagc agagaagttt gcgtacaagg taccttttta tggggatgcc cgcggagtct
    10141 tttgtgagat gttgaaaacg ccttcagcgg ggcagttttc attttttact gctcaccctg
    10201 gtattacgcg tggcggacat taccatcaca gtaaaaatga gaagtttttg gtcattcgag
    10261 gtcaggcatg ctttaaattt gaacatgtga ttaccggtga gcgatatgaa ctgaaagttt
    10321 catcgggtga gtttaagatc gttgaaacag ttcctggttg gacacatgac attacaaata
    10381 ttggaactga ggaattaata gtcatgctct gggcaaatga aattttcaac cgtgatgagc
    10441 ccgatactat tgcgaggcct ctataatgaa aaaattaaaa gttatgtctg ttgttggaac
    10501 ccgtcctgag attatccgtt tgtcgagggt tcttgctaag tttgatgaat actgcgagca
    10561 tgttattgtc catactggtc aaaattatga ttacgaatta aatgaagtgt tcttcaatga
    10621 cttgggtgtt cgaaaacctg attattattt aaatgcagcg ggtaaaaatg cggcggaaac
    10681 cattggccag gttattatta aggtagatga agtattagaa atcgaaaaac ctgaagcaat
    10741 actggtattg ggcgatacga attcatgtat ttctgccatt ccggccaaac gccgtaaagt
    10801 gcctatattt catatggaag caggtaaccg ttgtttcgat caacgcgtgc ctgaagaaac
    10861 caatagacgt attgttgacc atacggctga tatcaatatg acctacagtg atattgctcg
    10921 tgaatatctc ttggctgaag gtatcccagc tgatcggatc ataaaaactg gtagccctat
    10981 gtatgaggtt ctttcatatt atatgcccca aattgatggt tcagatgtgc tatcgcgttt
    11041 gaatctacag tctggtgagt tttttgtagt aagtgcgcat cgtgaagaga atgttgattc
    11101 tccaaaacag ctcgtaaagc ttgcgaacat tctaaatact gttgctgaaa aatataatct
    11161 tccagttatt gtctccacac acccaaggac acgtaaccga atccgtgagc aaggaattga
    11221 atttcattca aatataaatt tactgaaacc attgggtttc catgattata accacttgca
    11281 gaagaactca cgagctgtgc tttcagatag cggtactatc actgaagagt catccatcat
    11341 gaatttccca gcggtaaaca tccgggaagc gcatgagcgt ccggaaggct ttgaggaagc
    11401 atccgtcatg atggtggggt tagagtgtga acgcgtatta caagcgctgg atattctggc
    11461 aacacaaccg cgaggtgaag tccgtctttt acgtcaggtt agtgattaca gcatgccaaa
    11521 tgtgtcggat aaagttgtca gaattgttca ctcttacaca gattatgtta agagagtcgt
    11581 ctggaaagaa tattgatgaa acttgcttta atcatagatg attacctgcc caacagtact
    11641 cgtgttggtg caaaaatgtt tcatgaactt gctcaagaat ttatccagcg tgggcacgat
    11701 gttacggtaa ttactcctgg tacgggcatg caagaagaga tttcttttga tacctttcag
    11761 ggggtaaaaa catggcgttt taaaagcggg ccgctcaagg atgtaagtaa aattcagcga
    11821 gcggtcaatg aaacgctttt gtcctatcgg gcgtggaaag ccatcaaaaa atgggtaaaa
    11881 aaagagacct ttgagggtgt gatttattat tcaccttcca tattctgggg gcatttagtt
    11941 aaaaaaatta aagctcgttg ccaatgtcct gcttatctta ttttaagaga tatgtttcca
    12001 caatgggtaa ttgatgcagg aatgcttaat gctggttccc caatagaacg ctactttcgt
    12061 ctttttgaaa aaatatctta tcgtcaggca aatcgtattg gacttatgtc tgataagaat
    12121 cttgatgttt ttcggaaaga taataaaggc tatccgtgcg aagttttgcg taactgggca
    12181 tccctaacac caacgatcat acccaaggat tatataccac tacgtaagcg acttggccta
    12241 gaggataaaa ccattttctt ctatggtgga aacataggtc atgcacagga catgacaaac
    12301 ttgatgcgac ttgtgagaaa catggcagta tatcctcaag ctcatttcct atttattggc
    12361 cagggtgatg aagttgaatt aattaattca ttagcctctg agtgggcatt gacgaatttc
    12421 acctatttgc cctcggttaa tcaggatgaa tttaagttca ttttgtcgga aatggatatc
    12481 ggcttgtttt ctctttccgc tagacactct tcccataatt ttcctggtaa gttattaggc
    12541 tatatggttc agtcgctacc tattttaggt agcgtaaatg ccggaaatga tttgctcgac
    12601 attgtcaatc aaaataatgc cggattaatc catgtcaatg gtgaggacga taaattatgt
    12661 caatctgcgc tattaatgtt gcatgatatt gatgtgcgcc ggcaacttgg tttgggagcg
    12721 aatatattgt tgaaagaaca attctccgtt gagtctgcgg cacagacgat agaaatgagg
    12781 ttggaggcat gcaatgcgat taattgataa tgaccaactc gacaaattat atgatcaagc
    12841 cgggcaatcg gaacgtttac gttcccacct tatgatgcac ggctcgcatc aagaaaaggt
    12901 acagcgttta cttattgcat tagtaaaggg cagctatgtt gaaccgcatt atcacgcact
    12961 tcctcatcag tgggaaatgt tcatagttat ggaggggcaa cttcaggttt gtttgtatgg
    13021 tagaaatggt gaggttataa agcaatttat agcaggagat aatactggga taagcattgt
    13081 ggagttttct ccgggcgata tacacagtgt cgaatgccta tctcctcgtg ctcttatggt
    13141 ggaagttaaa gaggggccgt ttgactcttc ttttgcaaaa ttgttcgtgt gatgctcgtc
    13201 taaaatgcat cttctgctat ctacccaagc taaacctgag ttaacatcca taccatattt
    13261 caagccgcga atatcttgcg cggtgaccac ccctgacagg agtatacaat gccaagcaac
    13321 agatcggcgt cgtcggtatg gcagtg
//
