LOCUS       AB811600               16805 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain:
            Bi626-42.
ACCESSION   AB811600
VERSION     AB811600.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16805)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Bi626-42"
                     /db_xref="taxon:562"
                     /note="serogroup:O12"
     gene            <1..903
                     /gene="wcaM"
     CDS             <1..903
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAQ00630.1"
                     /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILIS
                     DHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVEN
                     GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK
                     GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKP
                     QHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKRETLLSLTNIHAVNEKGQSSV
                     DIDRINHHIVNVEKINFRLPERRE"
     gene            1066..1971
                     /gene="galF"
     CDS             1066..1971
                     /gene="galF"
                     /note="O012_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00631.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLESDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSV"
     CDS             2321..3280
                     /note="O012_02"
                     /gene="wfeQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00632.1"
                     /translation="MKSKLVSIIIPAYNAQDWIERCLVSCLNQSYQNIEIIVINDGST
                     DNTANILEKFNVNKLKIFHEKNSGVAIARRKGLDYSRGEYIFFLDSDDFINESALEIL
                     VTKIDNYNADVVIAQAKHLKKKSTFTSSFKIEIDPLYSFLNGSLPRTLWPLLFKRSVI
                     EKNLIPSQYTVSEDYVIMSKIYTLPIKIVVVNDCLYNYVKHTGSVTANITPQKYNDHY
                     KAHHYIEKQLFETLSDKYKISFIRNNLDFLYSLIIINSPYVSHQMEYIWGKYIKEDIN
                     KATRRLSLKKKIILKLNHVALMRLGPLRMIILLMNKLRRYNEN"
     gene            3353..4612
                     /gene="wzx"
     CDS             3353..4612
                     /gene="wzx"
                     /note="O012_03"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00633.1"
                     /translation="MNIVLKNSMYLVFMQMCNYAIPLITLPYLTRCLGFYQYGVLNTA
                     INIILYIILVIDFGFNLSATREISQKKNNFVAVSKIFTDVIFAKIILFFGVLVFTFLI
                     LKLIPDYQDVTRLVYFMLPQVLSSILFPLWLYQGYEKIGYAAFINVLGKCITIPLLLI
                     FVQNSKDVTNAAIILSWSSLVPLLIYYIRSNLPRTKFYTSRCRFKYLNRTLANALVVF
                     IGSIAVNLYTLSTPLVLSIVSDFDQVGYFVAADKIRAAFIGVFLIVGQAIYPRASALY
                     NEDIIAYNKFVISIIFYQIIVCTFSAIAFYYFMPEVAPIILSQNKLNVEELSVIIKLM
                     SPMIILIPLSVILANCVLLPQRKNKQYALVPIITVIIHMSYSIILCEKLGALGASYAI
                     LLTEIISCALLAYFTFKDRLIQLNPAR"
     gene            4625..5728
                     /gene="wzy"
     CDS             4625..5728
                     /gene="wzy"
                     /note="O012_04"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00634.1"
                     /translation="MNHSYIYVYIFFVFLSFFELNDTNRARCDLYYAIQVIVLLLTMG
                     LSYQIGVDWVEYERVYNGNSESLNNYEFGYIVLNNIANYLGASFWGFVILIKYTFFIS
                     LFLMVRKFSRLPVLTTTLILGLLFPFINDPLRQIISASILFFTIYFYNRPPKILGIIG
                     GGVFHSTFIIIIIRYLKIFTKRSIVLIFVFVGVAILLFLKYGSFLGDGMLFRKLNFYM
                     EYSSLSNLYGFALRFLLLSFIVFNIDVYKANCKFIRGDICYYFWIFSITYLWLEIIFV
                     TFPLIPQRMRLYLLPFAFILLSNYLYYSRLTFTKNILAFICVIISFSSLCLFLDGPMG
                     HFYSITDNIVLKYIQGFTNDKTYDANQFWINGI"
     CDS             5729..6826
                     /note="O012_05"
                     /gene="wbwG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00635.1"
                     /translation="MKKNVLIIASAAKEGGALSILTESLNNLKILNIHFTVVVNPSVV
                     KYLPVANNIKYHMVDTSAWIKRIAYDFYGYPGLKYEEYIACINFQNIPIRTKIKQIVY
                     YHQSLPLSDTLFSPFNKETRVLYLYQKFYGLFFKLNKRYISHLVVQANWIREKISEKF
                     GVENNKIEVIAPQVEIFKYISLEFNKIRPVENVFFYPANAYTYKNHLIIIKALNYLGS
                     VYIKKHRIKVVFTINNSESNYLQSMVLNYNLQDVVSFIGRIPRADVYSLLIKSKALLF
                     PSKLETYGLPLKEARQLGVFIVASKLDYATEVLEGYEYKRLCSPDSAKEWASAIKEII
                     ENKIDVGNTVTRDNIKESDLFSDFIKSKIME"
     gene            6833..7867
                     /gene="fnlA"
     CDS             6833..7867
                     /gene="fnlA"
                     /note="O012_06"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="BAQ00636.1"
                     /translation="MFAEKTLLITGGTGSFGNAVLRRFLDTDIKEIRIFSRDEKKQDD
                     MRKKYNNQKIKFYIGDVRDYSSILNASRGVDFIYHAAALKQVPSCEFHPMEAVKTNIL
                     GTENVLEAAIANRVRRIVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKKLLLKLPFIRAI
                     RSGEDYELDS"
     gene            7869..8975
                     /gene="fnlB"
     CDS             7869..8975
                     /gene="fnlB"
                     /note="O012_07"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2-acetamido-2,6-dideoxy-beta-L-talose
                     4-dehydrogenase"
                     /protein_id="BAQ00637.1"
                     /translation="MKILVTGASGFIGRNLVFRLKEAGYNELITIDRNSSLADLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNSNVTQQIVDILKKNSKNTPIMLSSSIQAECDNA
                     YGKSKATAEKIIQQYGETTNAKYYIYRLPNVFGKWCRPNYNSFIATFCHRIANDETIT
                     INDPSAVVDLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLITEDVGNGFTRALYSTWLSYLSPEQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIVIRGSACFKFENIVTGERYEFNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            8972..10102
                     /gene="fnlC"
     CDS             8972..10102
                     /gene="fnlC"
                     /note="O012_08"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="BAQ00638.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIIHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDDVLEQEKPEAMLVLGDTNSCI
                     SAIPAKRRKIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMPQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAERYNIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESERVLQALEIIATQ
                     PRGELRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     CDS             10102..11310
                     /note="O012_09"
                     /gene="wbuB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00639.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLGKGHDVTVITPDIT
                     LQAIYSVSMIDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWRALKHLIQHDTFDGI
                     VYYSPSIFWGDLVKKIKQRCQCPSYLVLRDMFPQWVIDAGMLKAGSPIEKYFRYFEKK
                     SYQQADRIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVCASDDYNSLRQKYDLKDK
                     VIFFYGGNIGHAQDMTNLLRLARNMMRYHDAHFLFIGQGDEVDLINSLAAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLADSALRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     CDS             11301..11708
                     /note="O012_10"
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ00640.1"
                     /translation="MRLVDDNILDELFRTAVNSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEKNGEIQKQFIVGDGTGISVVEFSPGD
                     IHSVKCLSPNALMLEIKEGPFDPLKAKAFSKWL"
     gene            11821..13227
                     /gene="gnd"
     CDS             11821..13227
                     /gene="gnd"
                     /note="O012_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00641.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNCELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            13476..14642
                     /gene="ugd"
     CDS             13476..14642
                     /gene="ugd"
                     /note="O012_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00642.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQADKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14732..15769
                     /gene="wzz"
     CDS             14732..15769
                     /gene="wzz"
                     /note="O012_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00643.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQ
                     AAPKVPDLQEALIGRFSSAFSALAETLDNQKEPEKLTIEPSVKNQQLPLTVSYVGQTA
                     EDAQMKLAQYIQQVDDKVNQELEKDLKDNLALGRKNLQDSLRTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQ
                     TRQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVL
                     GRNALRNYNAK"
     gene            complement(15865..16476)
                     /gene="hisI"
     CDS             complement(15865..16476)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00644.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
BASE COUNT     4980 a   3099 c   3596 g   5130 t
ORIGIN
        1 tacgccatct tacgccaggg atttcataac cagataatcg gtgccaacat caccaactgt
       61 aaatttagtg atttacaggg cgacgctatt gaatggaacg tggcgattaa cgacagtgat
      121 attttgatct ccgaccatgt catcgagcgc atcaactgta ccaacggcaa aatcaactgg
      181 ggcatcggta taggccttgc gggaagcact tacgataata attacccgga agaccaggca
      241 gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt gtcggcagtt gatacatgtt
      301 gaaaatggta aacattttgt tattcgtaat atcaaagccc gcaatatcac gccggatttc
      361 agtaagaaag caggtattga taacgccaca gtcgctattt acggttgtga caatttcgtg
      421 attgataata ttgaaatgat taatagcgcc gggatgttaa tcggctatgg ggtaattaaa
      481 ggcaaatatc tctcgatacc gcaaaatttc cgagtgaata atattcaact ggataacacc
      541 catcttgctt ataaattgcg cggcatccaa atctccgccg ggaatgctgt ctcctttgtg
      601 gcgctgacta acattgagat gaagcgtgcc tcgctggagc ttcacaataa accgcaacat
      661 ctttttatgc gaaatatcaa tgtgatgcag gaatcctcag ttggacccgc attgagcatg
      721 aacttcgaca tgcgcaaaga cgttcgcggc gtctttatgg caaagcgtga aacgctgctt
      781 tcactcacga atattcatgc cgtgaatgaa aaagggcaaa gctccgtcga tatcgacagg
      841 attaatcacc atattgttaa tgtggaaaag attaacttta gattgccaga acggagggag
      901 tagatttgcg accattcttg gaaaaatgga gccatactta ggaacaatgc tactgcaatc
      961 cacaacgaag cggcgtaaca tcacaagtaa ttcagtaatc aattcagggt aattgatgct
     1021 ggcgaaaaaa atcgaacaag ctataattca gcaaccattt tacaggtgga tgaaataatg
     1081 acgaatttaa aagcagttat tcctgtagcg ggtcttggga tgcatatgtt gcctgccact
     1141 aaggcgattc ccaaagagat gctaccgatc gtcgacaagc caatgattca gtacattgtt
     1201 gacgagattg tggctgcagg gatcaaagaa atcctcctgg taactcacgc gtccaagaac
     1261 gcggtcgaaa accacttcga cacctcttat gaattagaat ctcttcttga acagcgcgtg
     1321 aagcgtcagc tgctggcgga agtgcagtcc atctgtccgc cgggcgtgac cattatgaac
     1381 gtgcgtcagg gcgaaccttt aggtttgggt cactccattt tatgtgcacg acccgccatt
     1441 ggtgacaatc catttgtcgt ggtgctgcca gacgttgtga tcgacgacgc cagcgccgac
     1501 ccgctgcgct acaaccttgc tgccatgatt gcacgattca acgaaacggg tcgtagccag
     1561 gtgctggcaa aacgtatgcc gggtgacctc tctgaatact cggtcatcca gaccaaagaa
     1621 ccactggacc gtgaaggtaa agtcagccgc attgttgaat ttatcgaaaa accggatcag
     1681 ccgcagacgt tggagtcaga catcatggcc gttggtcgct atgtgctttc tgccgatatt
     1741 tggccggaac ttgaacgcac gcagccaggt gcatgggggc gtattcagct gactgatgcc
     1801 attgccgaac tggcgaaaaa acagtccgtt gatgccatgc tgatgacagg tgacagctac
     1861 gactgcggta aaaaaatggg ttatatgcag gcatttgtga agtatggact acgcaacctg
     1921 aaagaagggg cgaagttccg taaaggcatt gagaagctgt taagcgtata atgaaaatct
     1981 gaccggatgt aacggttgat aagaaaatta taacggcagt gaagattcgt ggcgaaagta
     2041 atttgttgcg aattttcctg ccgttgtttt atataaacaa tcagaataac aacgagttag
     2101 caataggatt ttagtcaaag ttttccagga ttttccttgt ttccagagcg gattggtaag
     2161 acaattagcg tttgaatttt tcgggtttag cgcgagtggg taacgttcgt cacatcgtag
     2221 acatgcatgc agtgctctgg tagctgtaaa gccaggggcg gtagcgtggg ttaattctaa
     2281 aattagaatt ttacgacact ataagtaaaa taggtttata atgaagagta aattagtttc
     2341 cataatcata ccagcataca atgctcagga ttggattgaa aggtgtttag tatcttgcct
     2401 taatcagagc tatcaaaata tagaaattat tgtaataaat gatggaagta cagataatac
     2461 tgctaatatt ctcgagaagt tcaatgtaaa taaactgaaa atatttcatg aaaaaaactc
     2521 tggtgtagca atagcaagac gaaaaggttt ggattattct agaggagagt atatcttttt
     2581 tcttgatagt gatgatttta taaatgagtc tgctttagaa atattggtaa ccaagattga
     2641 taattataat gctgacgtgg ttatagcgca agctaaacat cttaaaaaga aaagcacttt
     2701 tacatcatct tttaaaattg aaattgatcc actatattca tttttgaatg gatctttacc
     2761 aagaacttta tggccattat tatttaaaag atctgttatt gagaaaaatc taataccatc
     2821 tcaatataca gttagtgaag actatgtaat aatgtcaaaa atatatacct tgccaattaa
     2881 aatcgttgtc gttaatgatt gtctatacaa ttatgtaaaa cacacgggta gcgtaacagc
     2941 gaatataacg ccacagaaat ataatgacca ctataaagct caccattata ttgaaaaaca
     3001 gttattcgaa acgttatcag ataaatataa aataagtttt ataagaaaca atttagattt
     3061 tctttatagt ttaattatta tcaattcacc atacgtcagt catcaaatgg aatatatttg
     3121 ggggaaatat attaaagaag atattaataa agctacaagg aggctttctc tgaagaaaaa
     3181 aataatactt aaacttaatc acgtagcctt gatgagactt gggccgttaa gaatgataat
     3241 attattaatg aataaactaa gaagatacaa tgagaattaa aactagtaat ataaaaatga
     3301 taatgataat attttgataa tgacatagtt aagtcataaa aggttatttt ctgtgaatat
     3361 agtattgaaa aattcaatgt atcttgtttt tatgcagatg tgcaattatg ctattccctt
     3421 aattacgcta ccttatttaa caagatgctt ggggttttat caatatggtg tattgaatac
     3481 tgccatcaat attatactat acatcatctt ggttattgat tttggattta atttaagcgc
     3541 cacaagagaa atatcacaga aaaaaaataa ctttgtagcg gtatctaaaa tatttacaga
     3601 tgtgattttt gcaaaaatta tacttttttt cggtgtgctt gtttttactt tcctcatact
     3661 taaactaatt cccgactatc aagatgtaac aagattggtt tattttatgt tacctcaagt
     3721 cctatcttcg atattattcc cactgtggtt atatcaaggg tatgaaaaaa ttggatatgc
     3781 tgcatttata aacgttcttg gaaaatgtat aacaatccca ttgctattaa tctttgtaca
     3841 aaatagcaaa gatgtaacaa atgctgcaat tatattaagt tggtcttctt tagttccctt
     3901 attaatttat tatatacgta gtaatcttcc gcgaacaaag ttttatacct caaggtgtag
     3961 attcaagtac ttaaatagaa cattagctaa tgcactggtc gtttttattg gttccattgc
     4021 tgtaaacctg tatactttaa gcacaccatt agtattaagt atagttagtg attttgatca
     4081 agttggttat tttgttgcag ctgataaaat aagggctgca tttattggcg tttttttaat
     4141 tgtagggcag gcgatttatc ctagagcaag tgccctgtat aatgaagata taattgcgta
     4201 taataaattt gtcataagca ttatatttta ccaaattata gtatgtacgt tctctgctat
     4261 agccttttat tacttcatgc cagaggtggc accaattatt ttgagtcaaa acaaattaaa
     4321 cgtagaagag ttatctgtta taattaaatt gatgtcgcca atgattattt tgatacctct
     4381 ttcggtgata ttagctaact gcgtactttt gcctcaaagg aaaaataaac aatacgcttt
     4441 agtgcctatt attacagtca taattcatat gagttactct ataattttgt gtgaaaagtt
     4501 aggtgcgcta ggggcaagtt acgcaatact tctcacagaa attattagtt gtgctttact
     4561 agcatatttt acattcaaag atagactaat tcagctcaat ccagccagat aatattggat
     4621 aattatgaat cattcttata tctatgttta tatatttttt gtgtttcttt cattttttga
     4681 gcttaatgat actaatagag caagatgtga tttatattat gccatacaag ttattgtatt
     4741 attactaaca atggggttgt cgtatcaaat aggtgttgat tgggttgaat atgaacgtgt
     4801 ttataatggg aattcggaat cattaaataa ttacgaattc ggttatatag tattgaataa
     4861 tattgcaaac tatttagggg ctagtttttg ggggttcgtg atattaataa aatatacgtt
     4921 ttttatatca ttgtttctta tggtaagaaa attcagtaga ttacctgttc tgactacaac
     4981 attaatacta gggttgttat ttccatttat taatgaccct ttacgacaaa taatatctgc
     5041 aagtatattg ttctttacga tttatttcta taacaggccc cctaaaatat tggggattat
     5101 agggggtggg gtatttcatt ctacatttat tataattata attcgttatc ttaaaatttt
     5161 tacaaaaaga tctattgtgc ttattttcgt ttttgtgggg gtggcaattc ttttgttctt
     5221 aaagtatggt tcttttttag gtgatggaat gttatttagg aaattaaatt tttacatgga
     5281 gtattcatca ttatcaaatt tgtatggctt tgccctaagg tttttgctat tgtcatttat
     5341 tgtttttaat attgacgttt acaaagctaa ttgcaaattc atcagaggag atatctgtta
     5401 ttatttctgg atttttagta taacatattt atggcttgaa ataatatttg taacattccc
     5461 tttaatccct cagcggatgc ggctttattt gttaccattt gcttttatac tattaagtaa
     5521 ctacctttat tatagtcgat taactttcac aaaaaatatt cttgctttta tatgtgtaat
     5581 catttcattt tcttcattgt gtcttttttt agatggtcct atggggcatt tttatagcat
     5641 tactgataat atagtattaa aatatattca gggttttact aacgataaaa catatgatgc
     5701 aaaccagttc tggattaatg gaatttaaat gaaaaaaaat gtcttgataa tagcttctgc
     5761 agcaaaagaa ggtggggctt taagcatact tacagaaagt ttaaataacc ttaaaatatt
     5821 aaatattcat tttacagttg tggtcaatcc atctgtggtt aaatatttgc ctgttgctaa
     5881 taatataaaa tatcatatgg tagatacatc ggcgtggatt aaaagaatag catatgattt
     5941 ctatggctat cctggtctca agtatgaaga atatatagcg tgcataaatt ttcaaaatat
     6001 acctataaga actaaaataa agcaaatagt atattatcat cagtcattac ctctttcgga
     6061 tacattattc tcaccgttta ataaagaaac gcgagtttta tatttatatc aaaaattcta
     6121 tggtttattt ttcaagttaa acaaaaggta tatatcgcat ctagtagttc aggcaaattg
     6181 gattagagaa aaaatatctg aaaaatttgg tgttgaaaat aataaaattg aagttattgc
     6241 acctcaagtc gaaattttta aatatataag tttagagttt aataaaataa gacctgtaga
     6301 gaatgtattt ttttatcctg caaatgcgta tacttataaa aatcatttga ttataatcaa
     6361 agctttaaat tatttaggga gtgtttatat taaaaaacat cgtattaagg ttgtttttac
     6421 tataaataat agtgagtcaa attatcttca atcaatggtt ttgaattata atttgcagga
     6481 tgttgttagt tttattggtc gaattccgag agccgatgtt tattctctct tgataaaatc
     6541 aaaagctctt cttttcccaa gtaaattaga aacttatggt ttaccattaa aagaggcgcg
     6601 gcaactaggt gtatttattg tcgcatcgaa gttagattac gcaactgaag tattggaggg
     6661 atacgaatat aaaagattat gctcgccaga tagtgctaaa gaatgggctt cggctataaa
     6721 ggaaattata gaaaataaga ttgatgtcgg aaatacagtc actagagata atattaaaga
     6781 gagcgatttg ttttctgatt ttattaaaag taaaataatg gagtgattat ttatgtttgc
     6841 agaaaaaaca ttgcttatta ctggtggtac ggggtctttt ggtaatgctg ttcttagacg
     6901 tttccttgac actgatatca aagaaatacg cattttttct cgagatgaga aaaagcagga
     6961 cgatatgagg aaaaagtata ataatcagaa aattaagttc tatataggtg atgttcgcga
     7021 ttattcgagt atccttaatg cttctcgagg tgttgatttt atttatcatg cagcagctct
     7081 gaagcaagtg ccttcctgcg aattccaccc catggaagct gtaaaaacga atattttagg
     7141 tactgaaaac gttctagaag ctgcaatagc taatcgcgtt aggcgaattg tatgtctgag
     7201 tacagataaa gccgtatatc ctattaatgc aatgggcata tctaaagcaa tgatggaaaa
     7261 agtcattgtt gcaaaatcac gtaatcttga tagttcaaaa acagttatct gcggaacacg
     7321 ttatgggaat gtaatggctt cacgtggatc ggtcatccca ttgtttgttg atctaatcaa
     7381 atctggtaaa ccattgacca ttaccgatcc caatatgact cgtttcatga tgacgcttga
     7441 ggatgctgtt gatctggtcc tttatgcctt cgagcatgga aataacggtg atattttcgt
     7501 tcagaaagct cctgcggcaa caattcaaac attagccatt gcacttaagg aattgctaaa
     7561 tgcccatgaa catccaatca atattattgg aactcgacac ggggaaaaac tttacgaagc
     7621 gttattgagc cgagaggaaa tgatagcagc ggaagatatg ggtgattatt atcgtgttcc
     7681 accagatctc cgcgacttga actatggtaa atatgtggaa catggtgacc gtcgtatctc
     7741 ggaagtggaa gattataact ctcataatac tgagagatta gatgttgagg gtatgaaaaa
     7801 attactgcta aaacttcctt ttatccgggc aattcgttct ggtgaagatt atgagttgga
     7861 ttcataatat gaaaatttta gttactggtg cttcagggtt tatcggccgt aatttggttt
     7921 tccgccttaa ggaggctggt tataacgaac ttattacgat agatcgtaac tcttctttgg
     7981 cggatttaga gcagggactt aagcaggcag atttcatttt tcaccttgca ggagtaaatc
     8041 gtcctgtgaa ggagagtgaa tttgaagagg gaaatagcaa cgtaactcaa cagattgttg
     8101 atattctgaa aaaaaatagt aaaaatactc ctatcatgct gagttcttcc atccaggctg
     8161 aatgtgataa cgcttatgga aagagtaaag cgactgcgga gaaaatcatt cagcagtatg
     8221 gggaaacgac aaatgccaaa tattatattt atcgtttgcc gaatgtattc ggtaagtggt
     8281 gtcgcccaaa ttataactcc tttatagcaa ctttctgcca tcgcattgca aatgatgaaa
     8341 ctattacaat taatgatcct tcagcagttg ttgatctggt gtatatagat gacttttgtt
     8401 ctgacatatt aaagctattg gaaggagcga acgaaactgg ttacaggaca ttcggtccaa
     8461 tttattctgt tactgttggt gaagtggcac aattaattta ccgatttaaa gaaagtcgcc
     8521 aaacattaat caccgaagat gtaggtaatg gatttacacg tgcattgtac tcaacatggt
     8581 taagttatct gtctcctgaa cagtttgctt atacggttcc ttcttatagt gatgatagag
     8641 gggtattctg tgaagtattg aaaacgaaaa acgcgggcca gttttcgttc tttactgcgc
     8701 atccaggaat tactcggggg gggcattatc atcattccaa aaatgagaaa tttattgtca
     8761 tccgaggaag tgcttgtttc aaatttgaaa atattgtcac gggtgagcga tatgaattta
     8821 atgtctcctc agatgatttt aaaattgttg aaacagttcc ggggtggacg catgacatca
     8881 ctaataatgg ctcggatgag ctagttgtta tgctttgggc aaatgaaata tttaatcgtt
     8941 ctgaaccaga tactatagcg agagttttat cgtgaaaaaa ttgaaagtca tgtcggttgt
     9001 tgggactcgt ccagaaatta ttcgactctc gcgtgtcctt gcaaaattag atgaatattg
     9061 tgaccacctt attattcata ctggccaaaa ctacgattat gaattgaatg aagttttttt
     9121 caaagatttg ggtgttcgca aaccagatta ttttcttaat gccgcaggta aaaatgcagc
     9181 agagactatt ggacaagtta tcattaaagt tgatgatgtc cttgaacagg aaaaaccaga
     9241 agctatgtta gttcttggcg atactaactc ctgtatttca gcaataccag caaagcgtcg
     9301 aaaaattccg atcttccata tggaggcggg gaatcgttgt tttgaccaac gcgtaccgga
     9361 agaaactaac agaaaaatag ttgaccacac tgctgatatc aatatgacat atagcgatat
     9421 cgctcgtgaa tatcttctgg ctgaaggtgt accagccgat agaattatta aaaccggtag
     9481 cccaatgttt gaagtactca ctcattatat gccgcagatt gatggttccg atgtactttc
     9541 tcgcctgaat ttaacacctg ggaatttctt tgtggtaagt gcccacagag aagaaaatgt
     9601 tgatacccct aaacagctcg cgaaactggc gaatatactt aataccgtgg ctgaaagata
     9661 taatatcccg gtagttgttt ctactcatcc gcgcactcgc aaccgcatca atgaaaacgg
     9721 tattcaattc cataaaaata tcttgctact taagccatta ggatttcacg attacaacca
     9781 tctgcaaaaa aattcacgtg ctgttttatc ggacagcggg actattacag aagagtcctc
     9841 cattatgaat ttccctgcac tcaatatacg agaagcgcac gaacgcccgg aaggcttcga
     9901 agaaggggca gtaatgatgg ttggtcttga atctgagcgc gtgttacagg cattagaaat
     9961 tattgcaaca caacctcgtg gtgaattacg cttacttcgt caggtcagtg actatagtat
    10021 gccaaatgtt tcagataaag ttgtgcgtat tatccattca tatactgact acgttaaacg
    10081 ggttgtctgg aagcaatact aatgaaactt gcattaatca ttgatgatta tttgccccat
    10141 agcacacgag ttggggctaa aatgtttcat gagttaggcc tggaattgct gggcaaaggc
    10201 catgatgtaa ctgtaattac gcctgacatc actttacaag caatctattc tgttagtatg
    10261 attgatggta taaaggtttg gcgtttcaaa agtggacctt taaaggatat aggtaaggct
    10321 aagcgtgcca taaatgaaac tcttttatct tttcgtgcat ggcgtgcatt aaagcacctc
    10381 attcaacatg atacatttga tggtatcgtt tattattccc cctctatttt ttggggagac
    10441 ttggttaaaa aaataaaaca gcgatgccag tgcccaagct atctggtcct aagggatatg
    10501 tttccacagt gggtcattga tgcaggtatg ttgaaagccg gttcgccaat tgaaaaatat
    10561 ttcaggtatt ttgaaaaaaa atcatatcaa caggctgacc ggattgggtt aatgtctgat
    10621 aagaatcttg agatatttcg tcaggctaat aaaggttatc catgtgaagt tttacgtaat
    10681 tgggcctcaa tgactcctgt gtgtgccagc gatgattata attcccttcg ccaaaaatac
    10741 gatctaaaag ataaagtcat ttttttttat ggaggtaata ttgggcatgc tcaggatatg
    10801 acaaacttat tgcgccttgc gcgcaatatg atgcgttatc atgatgctca tttcctgttt
    10861 atagggcagg gtgatgaagt tgacctgatt aattctcttg ctgcagaatg gaatttaact
    10921 aatttcactc atctaccttc agtgaaccag gaagagttta aattaatttt atctgaagtt
    10981 gatgttggcc tgttctccct ttcgtctcgc cattcttcac ataatttccc ggggaaatta
    11041 ctagggtata tggttcaatc aatcccgatc cttgggagcg tgaatggcgg taatgatctg
    11101 atggatgtaa ttaataagca cagggccggg ttcattcatg ttaatggtga agatgataaa
    11161 ctgttcgaat ctgcacaatt gcttcttgca gattcggctt taagaaagca gttaggtcag
    11221 aacgctaatg tgttgttaaa atctcaattt tcggttgaat cggcggcaca tactatcgaa
    11281 gtccgactgg aggcaggaga atgcgtttag ttgatgacaa tattctggat gagctttttc
    11341 gcacagcagt aaattctgaa cgtttgcgcg ctcattattt attgcacgca tctcatcagg
    11401 agaaggttca acgtttactt attgcatttg tacgcgacag ctatgttgag ccccattggc
    11461 atgagttacc gcatcagtgg gaaatgtttg tcgtcatgca agggcaatta gaagtttgtt
    11521 tgtatgagaa aaatggtgag atacaaaaac agtttattgt tggagacggt actggaataa
    11581 gcgtcgtgga attttcccca ggtgatatac atagtgtcaa atgcctgtca ccgaatgccc
    11641 ttatgttgga gataaaggaa ggaccatttg acccactgaa agctaaggct ttttctaagt
    11701 ggttataggg cgatacacca ccctttattc ttctatctta ttctatacat gctgggttac
    11761 catcttagct tcttcaagcc gcgcaacccc gcggtgaaca ccccctgaca ggagtaaaca
    11821 atgtcaaagc aacagatcgg cgtcgtcggt atggcagtga tggggcgcaa ccttgcgctc
    11881 aacatcgaaa gccgtggtta taccgtctct attttcaacc gttcccgtga aaagacggaa
    11941 gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt actatacggt gaaagagttt
    12001 gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg
    12061 gatgctgcta ttgattctct caagccatac ctcgataaag gtgacatcat cattgatggt
    12121 ggtaacacct tcttccagga cacaattcgt cgtaactgtg agctgtctgc agaaggtttt
    12181 aactttatcg gtaccggtgt ttccggtggt gaagaaggtg cgctgaaagg accttccatc
    12241 atgcctggtg ggcagaaaga agcctatgaa ctggttgctc cgatcctgac caaaatcgcc
    12301 gccgttgctg aagatggcga accgtgcgtt acctatattg gtgccgatgg cgcaggtcac
    12361 tatgtgaaga tggttcacaa cggtattgaa tacggtgata tgcagctgat tgctgaagcc
    12421 tattctctgc ttaaaggtgg cctgaatctc tctaacgaag aactggcgca gacgtttacc
    12481 gagtggaata acggtgaact aagcagctac ctgatcgaca tcaccaaaga catcttcact
    12541 aaaaaagatg aagacggtaa ctacctggtt gatgtgatcc tggatgaagc ggctaacaaa
    12601 ggtaccggta aatggaccag ccagagcgcg ctggatctcg gcgaaccgct gtcgctgatt
    12661 accgagtctg tgtttgcacg ttatatctct tctctgaaag agcagcgtgt tgccgcatct
    12721 aaagttctct ctggcccgca agcgcagcca gctggcgaca aaggtgagtt catcgaaaaa
    12781 gttcgccgtg cgctgtatct tggcaaaatc gtttcttacg ctcagggctt ctctcagctg
    12841 cgtgcagcgt ctgaagagta caactgggat ctgaactacg gcgaaatcgc gaagattttc
    12901 cgtgctggtt gcatcattcg tgcgcagttc ttgcagaaaa tcaccgatgc ttatgccgaa
    12961 aatccgcaga tcgctaacct gctgctggct ccgtacttca agcaaattgc cgatgactac
    13021 cagcaggcgc tgcgtgatgt cgttgcttat gcagtacaga acggtatccc ggttccgacc
    13081 ttcgctgctg cggttgccta ttacgatagc taccgtgccg ctgttctgcc tgcgaacctg
    13141 atccaggcac agcgtgacta tttcggtgcg catacttata agcgcattga taaagaaggt
    13201 gtgttccata ctgaatggct ggattaatct gatttaacca acaataaaat tgaggcccgg
    13261 cgtatattgc accgggcttt tttttgccaa ttatcttata gactaaattt cactgcttaa
    13321 tattaactta ataaatatca gctattctta taaagaaaat ctgaattgtt tttcgttgcg
    13381 ttgaccatca aacaacgtag cgttaaaact tttagctctt atcaggatgc taaaaacatc
    13441 atgattcaca gttaagttaa ttctgagagc atgaaatgaa aatcaccatt tccggtactg
    13501 gctatgtagg cttgtcaaac gggcttctaa tcgcacaaaa tcatgaggtt gtggcattag
    13561 atattttacc gtcacgcgtt gctatgctga atgatcggat atctcctatt gttgataagg
    13621 aaattcagca gtttttgcaa gcagataaaa tacactttaa tgccacatta gataaaaatg
    13681 aagcctaccg ggatgctgat tatgtcatca tcgccactcc aaccgactat gatcctaaaa
    13741 ctaattattt caatacatcc agtgtagaat cagtaattaa agacgtagtt gagataaatc
    13801 cttatgcggt tatggtcatc aaatcaacgg ttcccgttgg ttttaccgca gcgatgcata
    13861 agaaatatcg tactgaaaat attattttct ctccggaatt tctccgtgag ggtaaagccc
    13921 tttacgataa tctccatcct tcacgtattg tcatcggtga gcgttcagaa cgcgcagaac
    13981 gttttgctgc gctgttacag gaaggcgcga ttaagcaaaa tatcccgacc ctgtttaccg
    14041 actccactga agcagaagcg attaaacttt ttgcaaacac ctacctggcg atgcgcgtgg
    14101 cgtactttaa cgaactggat agctacgcag aaagtttagg tctgaattcc cgtcaaataa
    14161 tcgaaggcgt ttgtctcgac ccacgtattg gcaaccatta caacaatccg tcgtttggtt
    14221 atggtggtta ttgcttgccg aaagatacca agcagttact ggcgaactac cagtctgtgc
    14281 cgaataacct gatctcggca attgtcgatg ctaaccgcac gcgtaaagat tttattgccg
    14341 atgccatttt gtcacgcaag ccgcaagtgg taggtattta tcgtctgatt atgaagagcg
    14401 gttcagataa cttccgtgcg tcttctattc aggggattat gaaacgtatc aaggcgaaag
    14461 gcgttgaagt gatcatctac gaaccggtga tgaaagaaga ttcattcttc aactctcggc
    14521 tggaacgtga tctcgccacc ttcaaacaac aagccgatgt cattatttcc aaccgtatgg
    14581 cagaagagct taaggatgtg gcagacaaag tctacacccg cgatctcttt ggtagtgact
    14641 aacatcctgt tatcagggcg attttcgccc tgattctctt atgttccctt tgtaataatt
    14701 cattattttt atcatttatc ctatagcatt catggcgatt atcgctaaac tatggcgact
    14761 taaaattatt tcgtccgtta gggtaatgat gagagtagaa aataataatg tttctgggca
    14821 aaaccatgac ccagaacaga ttgatttgat tgatttactg gtgcagttgt ggcgtggcaa
    14881 gatgacaatt atcatctctg tcattgttgc tattgccctg gctattgggt atttggcggt
    14941 agcgaaggag aaatggacgt caacagcaat tatcactcag cctgacgtgg ggcaaattgc
    15001 tggctataac aatgccatga atgttatcta tggtcaggct gcacctaaag tgccggattt
    15061 acaggaggcg ttaattggtc gcttcagttc tgccttctct gcattagcag aaacgctgga
    15121 taatcagaaa gaaccagaaa aacttaccat cgaaccttct gtaaagaacc agcaattacc
    15181 attgactgtt tcttatgttg ggcaaactgc agaggacgca caaatgaaat tggcccaata
    15241 cattcagcaa gttgatgata aagtaaatca agagctagaa aaggatctca aggacaacct
    15301 tgctttggga cggaaaaact tgcaggactc tttaagaact caggaagtgg ttgcgcagga
    15361 gcagaaagat ctgcgtatcc gtcagattca ggaagcgttg cagtatgcga atcaggcgca
    15421 ggtgacaaaa ccgcagattc aacagactgg cgaagatatc acacaagata cgttgttcct
    15481 tttggggagc gaagcgctgg agtcgatgat taagcatgag gcgactcgtc cgttggtgtt
    15541 ttcgccaaac tactatcaga ctcgtcaaaa cctgctggat atcgaaaact taaaggttga
    15601 cgatcttgat attcatgctt accgttatgt gatgaaaccg acgttaccta ttcgtcgcga
    15661 tagcccgaaa aaggcaatta ccttgattct ggcggtgctg ctgggcggca tggttggcgc
    15721 ggggattgtg ctggggcgta atgctctacg caattacaac gcgaagtaat cttttcggtt
    15781 ttaaagaaaa agggcagggt ggtgacacct tgcccgtttt tttgccggat gcggcaacaa
    15841 tatcgcatcc gcttaccccg caactcactg atgccgttta cgcaggttct caattaccgt
    15901 cgttaaatcc agcccctgat cctgcaacaa caccagcagg tgatacatca aatcagacgc
    15961 ctcgttggtc agctcaaagc ggtcatgtac cgttgctgcc agcgcggttt ccacgccttc
    16021 ttcacccact ttctgcgcaa tgcgtttggt gccgctggca tacagtttgg cggtgtagga
    16081 ggtttccgga tcggcagatt tgcgctcggc gagcagttgt tccagttgat acaggaacag
    16141 ccactggtga gcggtgtcgc cgaagcaact gctggtgcct ttgtggcagg tcgggccgat
    16201 gggattcgcc agcaccagta acgtgtcgtt gtcgcagtcc ggggcaatac tcactacgtt
    16261 gaggaaattg cccgacgttt cgcctttggt ccacagtcgc tgtttagtgc gcgagaagaa
    16321 ggtgactttg ccgctttcga gggttttgtc taaggcttcc gggttcatat agcccagcat
    16381 tagcacttcg ccggataccg cgtgttgcac aatcaccggc ataagtccgt cggttttttc
    16441 ccagtccagt tcgcgacgtt gttgttctgt taacatatcc tgatctccac gccctgtgtt
    16501 gccaggtacg cttttaattc accaatattg attatttgtt tgtggaatac ggaagctgcc
    16561 agcgcgccgt caacgtcggc atcgcggaag gcttcgagga agtgttccat ggtgcccgcg
    16621 ccaccggagg caatcagcgg gacgtggcaa acttcacgca cttttttcag ttgttcgagg
    16681 tcgtaaccgt tacgcacgcc gtcctgattc atcatattga ggacgatttc tccggcaccg
    16741 cgtttttgca cttcctgtac ccagtcgagc gtttcccatt gagtgacgcg ggtgcggctt
    16801 tcatc
//
