LOCUS       DQ676933               15783 bp    DNA     linear   BCT 06-FEB-2007
DEFINITION  Escherichia coli strain 43w serogroup O123 O antigen gene cluster,
            complete sequence.
ACCESSION   DQ676933
VERSION     DQ676933.1  GI:110666541
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Beutin,L., Wang,Q., Naumann,D., Han,W., Krause,G., Leomil,L.,
            Wang,L. and Feng,L.
  TITLE     Relationship between O-antigen subtypes, bacterial surface
            structures and O-antigen gene clusters in Escherichia coli O123
            strains carrying genes for Shiga toxins and intimin
  JOURNAL   J. Med. Microbiol. 56 (PT 2), 177-184 (2007)
   PUBMED   17244797
REFERENCE   2  (bases 1 to 15783)
  AUTHORS   Beutin,L., Wang,Q., Naumann,D., Han,W., Krause,G., Leomil,L.,
            Wang,L. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2006) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="43w"
                     /serotype="1"
                     /db_xref="taxon:562"
                     /note="serogroup: O123"
     gene            108..1181
                     /gene="rmlB"
     CDS             108..1181
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABG81781.1"
                     /db_xref="GI:110666542"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNSNEALPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVMTQGVVGETYNIGGRNEKKNLDVVNTICDL
                     LDEIVPKQGSYRDQIIYVTDRPGHDRRYAIDASKISDELGWKPQETFESGIRKTIGWY
                     LNNLEWCRRVQDGSYHRERLGLQ"
     misc_feature    108..15593
                     /note="O antigen gene cluster"
     gene            1182..2051
                     /gene="rmlA"
     CDS             1182..2051
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABG81782.1"
                     /db_xref="GI:110666543"
                     /translation="MKGIILAGGSGTRLYPVTMAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRNILIISTPQDIPRFMGLLGDGSQWGLQLEYKIQNSPDGLAQAFILGEDFIGNDNC
                     ALILGDNIFYGHDLQKHLEIALSKDKGATVFAYHVKDPMRYGVVEFDKQGKAISLEEK
                     PEIPKSNYAVTGLYFYDNNVVEIAKSLKPSKRGELEITDVNRLYLERGELSVAMMGRG
                     YAWLDTGTHESLIEASNFIQTIEARQGLKVSCPEEIAFNKKFIDKTQLIKLAKPLEKN
                     SYGKYLIKLAESN"
     gene            2169..3614
                     /gene="wzx"
     CDS             2169..3614
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABG81783.1"
                     /db_xref="GI:110666544"
                     /translation="MNSNVKKNISAVNGLKWSAIERICSQGIQLLLMIVLARQLGPGA
                     FGLIGMLTIFITIGQVFIDSGFSAALIRKNERTESDYATVFYFNMTVAILFYAVLFFC
                     APFIAEFYKRNELIELTRVLGLTIIISAFIIVQRIQLSVILDFKTQAISSLSSVIISG
                     GCALLMAYNGFGVWSLVIQTITMGLVNLVILNIYNPWLPKRSFSKKSFHGFFSFGSRL
                     LISSLIDSIYTNIYLVVIGKSFSASTLGQFTQANLLSNTPAMTLTTVVQRVTYPLLSN
                     VNNAKGNIDEIYLRILRLTAAAVFPVMFLLAIIAKPFVVLFLGQQWEPVAELMSILCI
                     GYCLYPVHAINLNLLQVKGRTDLFLKLEIIKKTLITVILIVTIPYGVKIICIGIFAQY
                     YISLLINTYYTGKLSSLSAIAQIKALLPIWLMASISSAISWFLIPREIFSELYQIIGI
                     LITNISLYGIGMYLFQKDIYEMVKFLFIKTK"
     gene            3619..4749
                     /gene="vioA"
     CDS             3619..4749
                     /gene="vioA"
                     /note="dTDP-viosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="VioA"
                     /protein_id="ABG81784.1"
                     /db_xref="GI:110666545"
                     /translation="MNKEMKMLNGKILVTQPFLPELREFIPYLEKIWENKWLTNNGPF
                     HQQLENDLCRYLGVEYVSLFNNATIALITAVQSLELTGEVITTPYSFVATTHSLMWNN
                     LNPVFVDVSRDTFNINPSQIEAAITEKTTAIMAVHCYGNPCDVIAIEKIAKKYKLKVI
                     YDAAHAFGVNFKGESLLKYGDLSVVSFHATKVFNTFEGGVIICPNAETKLKIDQLKNF
                     GFEDELTIKSIGINGKMSEVNAAFGLVQLKHVNEAISKRKEINDLYGKLLGNVKGISL
                     AKFDKLATKNFSYYPILIEDDYGMSRDELCHLLQKNNIFARKYFYPLISDMDLYKNME
                     SARKENLHIARDISNKVLCLPIYADLDLDIVRFIARVIGNKK"
     gene            4746..5453
                     /gene="wfbA"
     CDS             4746..5453
                     /gene="wfbA"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbA"
                     /protein_id="ABG81785.1"
                     /db_xref="GI:110666546"
                     /translation="MKLAIMQPYLFPYLGYYQLMSSVDKFIIYDDVSYIKNGWINRNR
                     ILVNGNAHYFTVPVIGGSCNNKINTVKIDKTKKKAINKIIITIEQAYKKSVFFDEVFP
                     VIYGVLSKEYDFISDLAITSLLSIKNKLDIGAEVVLTSTNYGNNNLTSQDRVIDINVK
                     EHASTYINSEGGRLLYDKKTFKLNGVNLKFIHPEILPYKQLCNGEFVPSLSIIDVVMN
                     NGWDTTKQLVNSFELKD"
     gene            5457..6431
                     /gene="wfbB"
     CDS             5457..6431
                     /gene="wfbB"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbB"
                     /protein_id="ABG81786.1"
                     /db_xref="GI:110666547"
                     /translation="MREHNYAIGGYFSLELQALKNNTANEKVYLQSARACFQLLLESI
                     EVSRVWLPYYICDVVVDTINEIGIEILYYSISKDFIPQTFPVLEENDVFVYVNYFGVC
                     DEQTKLILQKYPPEKVILDNSQAFYSGHNNNLGTIYSPRKFFGVPDGGILITNQTIIL
                     PSSQDNDSSQYINHLIGRLISHPSEYYTDYIKAEERLKKIKKAKVMSYLTRKLLDSIN
                     YHEIKKIRDDNFKFLHHALEKINGIKIPEIVNGPLCYPLLSKNNKLKDILIKNEIYVP
                     TYWKDVLNRVDINSTEFEFVSNLIPLPCDQRYSSIQMKKIINIVLEEN"
     gene            6431..6997
                     /gene="wfbC"
     CDS             6431..6997
                     /gene="wfbC"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbC"
                     /protein_id="ABG81787.1"
                     /db_xref="GI:110666548"
                     /translation="MNIIGKTVKLRAVEIDDLELLNKWANDPEIWYMLGGWHFPYSKN
                     NTEKWIKNIDNNDSKNQIFAIETEEHGLIGTANLVNIDWKNKNAFHGIMLGNVETRGK
                     GYAQDVVMSLMRYAFDELGLNRLDGDMIEYNKLSINFYIKRCGWKIEGIKKEWFFRKG
                     QYFDKVVVGITKKEYLEHIEKSKYWETK"
     gene            6994..7395
                     /gene="wfbD"
     CDS             6994..7395
                     /gene="wfbD"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbD"
                     /protein_id="ABG81788.1"
                     /db_xref="GI:110666549"
                     /translation="MMERFKLGDTATYTQTITDADIKSFAGISGDNNPVHMSDEYAEG
                     SRFKKRIAHGLISASFFSALFGTKLPGPGCVYVNQSLKFLRPVYINDTVTARVVLTDI
                     DVVKRRLFFDTICEVNRKKVITGKAEIYLPE"
     gene            7469..8494
                     /gene="wfbE"
     CDS             7469..8494
                     /gene="wfbE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbE"
                     /protein_id="ABG81789.1"
                     /db_xref="GI:110666550"
                     /translation="MNVSMSDIKVSVCIISFNQQNYIRQCLDGVFSQKTNFEYEVIIR
                     DDCSTDNTYLTIMEYIDTLDEEKKKNIKITVLDGTKNIGANNNFIETFKTSVGQWLAI
                     CEGDDYWCDQGKLQKQYDYAISHSDCSLVVHPALISENNVIRKTSWACMNKTINQLSD
                     VIRAKGQFSPTGSYFFKREILNVLPLWFSTAPVGDYYMEIFATSLGSCHTIPDAMSVY
                     RINSTGSWSDLLKKDRNGQRIINTYLSQLEYLDKLAEIFPSCVDDITIKRSHAQYAAA
                     MGYLANGDYTNFRILMDKSDTNGWYDNMHSIFYYLRNSRALSMLMFRFKPAIKSAVMW
                     GRRVFNN"
     gene            8478..9728
                     /gene="wzy"
     CDS             8478..9728
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABG81790.1"
                     /db_xref="GI:110666551"
                     /translation="MYLTTNYCDFLSRTRIVRRIFLLVYLISNVYAYIVFTETGVLIG
                     DYQGVLIIYKDQVVYLLMLTIMSYYIVCGPVFNCLSKVKIRLRTFKGLNSFAYILFFF
                     QILFAVFNISTGSNAAGTDFQTGGVIRLLWLFLPVDYLFYIYYFVGREKKVSKIYLAN
                     VVIFILSMLSRGWLGWTLVLLYAELCFFFYSQKKIKIKYLILLFFLLIVAPLAFSLKI
                     QLRADLYSSGIGGVISTLSNIDYIQSYNNFIAGFLSRIQQLSNIVFFYDHQQELYKFV
                     SSDIVSNYAWEGLPQQTVAKLLGLDPGVDMHIFLYSHYISSTSEAVTTLQVGFISWLF
                     LGTLSSVFYPLFVFAIICISLFLSKKLGGEKLCALTWIMIFLSIMCGWYNAYLVYMQA
                     LITFYFIMGFLNLITLEKTKINHT"
     gene            9725..10846
                     /gene="wfbF"
     CDS             9725..10846
                     /gene="wfbF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbF"
                     /protein_id="ABG81791.1"
                     /db_xref="GI:110666552"
                     /translation="MMDDEKYMSNLVIVNATALASSGALTILNQFLEHALNDSKHKYL
                     CFIHESVKKKSVNNVTVITIKKQSFIQRVWWDLYGLNKYIKKNNLKPKKIVSLQNTSV
                     NSNFEQIIYLHQSIPFSNFRIKIKFQYAIFFMYKYVYPFFIFFRTKNTTFVVQTDWMK
                     DAIVAKKKIAKERVHVIKPDIILPVNNLAYPDEHDKDNSSEVFFLYPATPLFYKNHLI
                     ILDAMRILKTEGILANTKFQVTFKQDDNDELAIKIAKYDLVDNISFLGVLSYEELFHK
                     YLKADAILFPSYLESFGLPLAEGAMLGKYIICSDLPYARDVLNNYSNVEYINHDDASK
                     WALAMKEIILKNRNNTLSESENSYMYCPKTSWADFFELI"
     gene            10859..11890
                     /gene="fnlA"
     CDS             10859..11890
                     /gene="fnlA"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ABG81792.1"
                     /db_xref="GI:110666553"
                     /translation="MFKNKVLLITGGTGSFGNAVLQRFLDSDIGEIRIFSRDEKKQDD
                     MRKKYASDKLKFYIGDVRDYSSVLTATRGVDFIYHAAALKQVPSCEFYPMEAVKTNVI
                     GTDNVLEAAIANKVSRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPKDITICA
                     TRYGNVMASRGSVIPLFINQILEGRPITITDPCMTRFMMTLDDAVDLVLHAFEHGTNG
                     DIFVQKAPAATIDTLTKSLLKLTKQHSHPINIIGTRHGEKLFEVLCSREEMLVAEDQG
                     NYYRIPSDKRDLNYEKFFDKGTKEIQFVEDYNSHNTRRLDVDEMVALLRKLDYINKIE
                     AGEKADPDA"
     gene            11883..12752
                     /gene="qnlA"
     CDS             11883..12752
                     /gene="qnlA"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="QnlA"
                     /protein_id="ABG81793.1"
                     /db_xref="GI:110666554"
                     /translation="MRKKILIVGANGMLGSSLLRYFSSIGDYEVLGTTRSMVVAKQLE
                     QKHNVKIIDNVDVIDFKRLETVVVEHKPNIVFNCVGIIKQLDAAKNNILSIEINSLLP
                     HKLAQLCSAHSAKLIHFSTDCIFKGTKGNYVEDDESDAIDLYGKSKFLGEVEYNGHLT
                     LRTSIIGHELGSNHSLVDWFLSQKKSVKGFTNAIFSGLPTCYMAEVIHKYVLPNNLAG
                     LFHLSVEPISKYDLLNIIKIVYGVSTDIEPTNEFKIDRSLNSTLFRNKTNFVPESWDK
                     LIEKMKDEYNKYF"
     gene            12730..13884
                     /gene="qnlB"
     CDS             12730..13884
                     /gene="qnlB"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="QnlB"
                     /protein_id="ABG81794.1"
                     /db_xref="GI:110666555"
                     /translation="MNTINISNSKIRVVTVVGTRPEIIRLSRVIAVLDEYTEHFLVHT
                     GQNYDYELNEVFFNELEIRKPDFFMNAAGQNAAETIGNVIIEADKIFDKLQPEALLIL
                     GDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHISDINLTYSEIARD
                     YLLREGLPADQIIKTGSPMREVLNFYKDKISSSSILEKLNLQASSYFLVSSHREENVD
                     SPEKLRSLIETLNIVSEKYKLPVIVSTHPRTRNRIDALGITVSNNIIFSKPFGFLDYI
                     KLQQNARVVLSDSGTITEESSVLNFPALNLREVHERPEGFEEAVVMFVGLDRNRIIQG
                     IDILQGQKRGDNDRDLHMVTDYQADNVSIKILRIIMSYTNFINQKVWKKF"
     gene            13884..15077
                     /gene="wbwH"
     CDS             13884..15077
                     /gene="wbwH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwH"
                     /protein_id="ABG81795.1"
                     /db_xref="GI:110666556"
                     /translation="MRIALICDDYLPDSTRVSAKMMHELACELLEKGHEPIVICPCNK
                     IQTLEILNLDGVVVYKFPNGAIKNVSKISRAINESMLSFNAWRFIGKYIRETKIDGVV
                     YYSPSIFFGKLANKIKENWHCKSYLILRDSFPQWLVDQGIIKEGGLAERYFRYFEQIN
                     YDAADYIGLMSDRNKDIFINKYQNKYKVQTLFNWADFKGIDNIPSATLRSKLALQNKV
                     IFFYGGNIGHAQDMMNLMRLVRSASYRDDVHFLLIGQGDEVSLVKQFIIDNSLKNCTY
                     LPSITQSEFKSVLKIVDVGLFSLAKNHTVHNFPGKLLGYMANKLPILGSVNASNDVME
                     IINGAKAGFVFVNGNDEALLNAAINLADDTQLRKNLGCNAYSLLQEKFSVEMAAEKIL
                     SSLFS"
     gene            15096..15593
                     /gene="wbuC"
     CDS             15096..15593
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ABG81796.1"
                     /db_xref="GI:110666557"
                     /translation="MKVSFVSQDDFENLIQEAFASVRLRSHLLLHESPNDAVQRIMIG
                     LVKGTYIPPHFHEFQHQWEHFHVFQGEVELMLFDSNGCLNKKVILGGQSKNIIAQISP
                     LTPHTLVCRSPTAVIMEIKEGPFDEKCAKVIPSWSYSEDYSILSRDRIIAMMGQLSIG
                     DRFSL"
BASE COUNT     5279 a   2295 c   2995 g   5214 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactaag
       61 agccgctaat ttaacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttctatcact ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt agaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gtttccgttt tcatcatatt tctactgacg aagtatatgg cgacttaccc catcctgatg
      541 aagtaaatag taatgaagcg ttaccgctat ttacggaaat gacagcttat gcaccaagta
      601 gcccatattc tgcttctaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgtt ctaacaatta tggtccttat catttcccgg
      721 aaaaactgat tccattggtt attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gtaaggggga ccaaattcgc gactggttgt atgtagaaga tcatgcccgg gcattgtaca
      841 ccgttatgac ccagggtgta gtaggtgaaa cctacaatat cggtggtcgt aacgagaaaa
      901 aaaacctgga cgtggtaaat actatttgcg atctgcttga tgagattgta cctaaacaag
      961 ggtcttatcg cgatcaaatt atctacgtta ctgaccgtcc tgggcacgat cgccgctatg
     1021 cgattgatgc atcaaaaatc agcgatgagc tgggctggaa accgcaggaa acctttgaat
     1081 cgggaattcg aaaaactatt ggttggtatt taaataattt agagtggtgc cgtcgtgtgc
     1141 aagacggtag ctatcatcgt gaacgtttag gattacagta aatgaaaggt atcatacttg
     1201 caggtggttc aggaactcgt ttatatccag tcacaatggc tgtaagtaaa caattgttgc
     1261 cagtttatga taaaccaatg atttattacc cattaagtac attaatgttg gctggtatac
     1321 gaaatatact tattataagt acacctcaag atattcctcg ttttatgggg ttattaggtg
     1381 atggtagtca atggggactt cagttagaat acaaaataca gaatagtcct gatggattag
     1441 cgcaagcatt tatattaggt gaggatttta ttggaaacga taattgtgca ttgattttag
     1501 gggataatat attctatgga catgatctcc agaaacacct agaaatagcg ctctcaaaag
     1561 ataaaggtgc tacagttttc gcttatcatg ttaaagaccc tatgagatac ggcgttgtag
     1621 agtttgataa acaaggtaaa gccatttcac ttgaagagaa acctgaaatt ccaaaaagta
     1681 attatgcagt aacgggattg tatttttatg ataataatgt tgttgagata gcgaaatcat
     1741 taaaaccctc gaagcgagga gagctagaaa taacggatgt taatcgactc tatttggagc
     1801 ggggtgagct ttctgtagct atgatggggc gaggttatgc ttggctagat actggaactc
     1861 atgaaagtct aattgaagct agcaatttca tccagacaat tgaagctaga cagggtttaa
     1921 aagtatcctg ccctgaagaa atagcattta ataaaaaatt tattgataaa acgcagttaa
     1981 taaaattagc taagcctttg gagaaaaatt catacggaaa atatttaata aaactggcag
     2041 agtcgaattg attttttatg aatgattaat ttgttcatag aataaaaagt gaatgcctcc
     2101 atggttaatg agttaattta acaactaaga aggtgagtat ctatctctaa gtgttattga
     2161 gaaggtatat gaattcaaat gtaaaaaaaa atattagcgc tgtaaatgga ttgaagtgga
     2221 gtgcgatcga aagaatatgt tcacaaggta tccagctact tttaatgata gtcttggcta
     2281 gacaattagg gcctggtgca tttggcctta ttggaatgct gacaatattt attacaatag
     2341 gtcaggtctt tattgatagt ggttttagtg ccgctctcat tcgaaaaaat gaaagaacag
     2401 aatcagacta tgcaactgtt ttttacttta acatgacagt tgccattctg ttttatgcag
     2461 tgttattttt ttgcgcccca ttcatagctg aattttataa gcgtaacgaa ttaattgaat
     2521 taacaagagt tctgggttta acaataataa taagtgcttt tattattgtt caacgaatac
     2581 aattaagtgt cattttggat ttcaaaactc aagctatatc gtcattatcc agtgtcataa
     2641 tctcaggagg gtgtgcacta ttaatggcat ataatggttt tggtgtatgg tcgttagtta
     2701 tacagactat taccatgggg cttgttaatt tagttatttt aaatatatat aatccatggt
     2761 taccgaagag gagtttttca aaaaaatcat ttcatggatt tttttctttt ggctccagac
     2821 ttctgatttc atcactgata gattcaatat acactaatat ttatttggta gttataggga
     2881 agtcctttag cgctagcaca ctgggccaat ttacacaagc taatttatta tcaaatacgc
     2941 cggccatgac gttaacgaca gttgtacaaa gagttaccta tccattatta agtaatgtga
     3001 ataatgctaa ggggaatatt gacgagatat atcttaggat attaaggctt actgccgcag
     3061 ccgtttttcc agtaatgttc ctattggcaa taattgctaa accttttgtc gttctatttc
     3121 ttggccaaca atgggaacct gttgcggaat taatgagcat attatgtata ggatactgtt
     3181 tatatccagt acatgctatt aatctaaact tattacaggt aaaaggacga actgatttat
     3241 ttttgaagtt agaaataata aaaaaaactc tcatcacggt tattctaata gtaacaatac
     3301 catatggtgt taaaataata tgtataggta tttttgcaca gtattatata tccttgttga
     3361 taaatacata ctatacaggg aaactcagca gcttaagtgc aattgcacag ataaaggcat
     3421 tattgccaat ttggttaatg gcatctatca gttcggcaat tagttggttc ttaataccaa
     3481 gagagatatt ttcggaatta tatcaaataa taggaatatt gataaccaat atttcattat
     3541 atggaatagg aatgtatctt ttccaaaaag atatttatga aatggtaaag tttttattta
     3601 taaaaacaaa ataattttat gaacaaagag atgaaaatgt taaatggcaa gattttagta
     3661 acgcaaccat ttttacctga actaagagag tttattccct atctggaaaa aatatgggaa
     3721 aataaatggt taacaaacaa tggtccattt catcagcaat tagaaaatga tttgtgccgt
     3781 tatttaggcg tggagtacgt ctccttattt aataatgcta caattgctct gattacggca
     3841 gtccaatcat tagaattgac tggtgaagta attacaaccc cgtattcatt tgtggcaaca
     3901 actcactcat tgatgtggaa taatctaaat ccagtatttg tcgatgtcag tagagataca
     3961 tttaatatca atccgtctca aattgaagcg gcgataacag aaaaaacaac agccataatg
     4021 gcagtccatt gttatggtaa tccttgtgat gtgattgcaa tagaaaaaat agccaaaaag
     4081 tataaactta aagtgatata tgatgctgct catgcttttg gggtaaattt taagggggaa
     4141 agtttattaa aatacggaga tttatcggtt gttagttttc atgcaactaa agtatttaat
     4201 acatttgaag gtggcgtgat tatttgccca aatgcagaaa ctaagctcaa aatagatcag
     4261 ctaaaaaact ttggttttga agatgagcta acaataaaat caattggtat taatggaaaa
     4321 atgagtgagg tcaatgcagc atttggcctt gtacagttga aacatgttaa tgaagctata
     4381 agtaaaagaa aagaaattaa tgatttatat ggcaagttat tagggaatgt gaaaggcata
     4441 tcattagcaa aatttgataa actagctacg aaaaattttt catactaccc gattcttatt
     4501 gaagatgact atgggatgag tcgagatgaa ttatgtcatt tacttcaaaa aaataatata
     4561 tttgctagga aatattttta tcctttaata agcgatatgg atttatataa aaatatggag
     4621 tcagcgagaa aagaaaatct acacattgct cgagatattt ccaacaaagt actttgctta
     4681 ccaatttatg cagatcttga tttagacatt gtgagattta tagcgagagt aataggtaat
     4741 aaaaaatgaa attagcaata atgcaaccct atctatttcc ttatctaggt tattatcaat
     4801 taatgtcatc agttgataag tttattattt atgatgatgt ttcatacatc aaaaatggtt
     4861 ggataaacag aaatagaatt cttgttaatg gtaatgctca ttattttact gtgccagtta
     4921 taggtgggag ttgtaataat aaaataaata ctgttaaaat tgacaagaca aagaaaaaag
     4981 ctatcaataa aatcattatt acaattgaac aagcctataa aaaatccgtt ttttttgatg
     5041 aggtctttcc agtcatttat ggtgtgttat ctaaagagta tgatttcata tccgatctgg
     5101 caataacgtc attattgtca ataaaaaaca aacttgatat tggagcagaa gttgttttga
     5161 cttcgaccaa ctatggtaac aataatttaa cttcgcaaga tcgcgtcatt gatattaatg
     5221 taaaagagca tgcgtccact tatattaatt ctgaaggcgg gagattactt tatgataaaa
     5281 agacattcaa actaaatggc gtaaatttga aatttataca tccagaaatt ttaccttaca
     5341 aacaactttg taatggtgag tttgtaccat ccttatcaat tatagatgta gtaatgaata
     5401 atggttggga tactacaaag cagttagtaa atagctttga actgaaggat tgaacaatgc
     5461 gtgagcataa ttatgcgatc ggaggatatt tttcactaga attgcaggcg cttaaaaata
     5521 acactgcaaa tgaaaaagta tatttacaat cagcgagggc atgttttcaa ttactgctag
     5581 aaagcattga agttagtaga gtatggttac catattatat atgtgatgtc gtcgttgata
     5641 caataaatga aattgggatt gaaatcttgt attatagtat ttctaaagat tttattccgc
     5701 agacgtttcc agttttagaa gaaaatgatg tttttgtcta tgttaattat tttggggtgt
     5761 gtgatgagca aacaaagtta attttgcaaa aatatcctcc agagaaagtt atcttagata
     5821 actcacaagc tttttatagc ggacacaata ataatttagg aacaatttat tcacctagga
     5881 aattttttgg cgtccctgat ggtggtattc tgataactaa tcagactata atattacctt
     5941 cttcacagga taatgattca tcacagtata taaatcatct cattgggcgt ctaatatccc
     6001 atcccagcga atactatact gattatatta aagctgaaga acgattaaaa aaaattaaaa
     6061 aagccaaagt gatgtcttat ttaactcgga aattattaga ctccatcaat tatcatgaga
     6121 ttaaaaaaat aagggatgat aattttaaat tccttcacca tgccctcgaa aagataaatg
     6181 gaataaaaat tccggagata gtcaatggtc cattatgcta tccattgctt tcaaaaaaca
     6241 acaaactgaa agatatttta atcaagaatg aaatttatgt gcctacatat tggaaagatg
     6301 tattgaatcg agtagatata aactcgacag aatttgaatt tgtctcaaac ttaatacctc
     6361 tgccatgtga tcaacgttac tcatctatcc agatgaagaa aataatcaat attgtacttg
     6421 aggaaaacta atgaatatca taggaaaaac agttaagttg cgtgctgtgg aaattgatga
     6481 tttagaattg ttaaataaat gggctaacga tccggaaatt tggtatatgc ttggtggttg
     6541 gcatttcccc tattccaaaa ataatactga aaaatggata aaaaatattg ataataatga
     6601 ttcgaaaaat caaatattcg ctattgaaac tgaagagcat ggattgattg gcactgctaa
     6661 cttagtaaat attgattgga aaaataaaaa tgcatttcat gggataatgt tgggaaatgt
     6721 tgaaacacga ggtaaagggt acgcgcaaga cgttgtaatg agtctgatga gatatgcctt
     6781 tgatgaatta ggattaaatc gacttgatgg tgatatgatt gaatataata aattatcaat
     6841 taatttttac atcaaaagat gtggttggaa aatcgaggga attaagaaag agtggttctt
     6901 tagaaagggg caatattttg ataaagtagt tgttggaata accaaaaaag aatatttaga
     6961 acatattgag aaaagcaagt attgggagac aaaatgatgg aacgttttaa attgggggac
     7021 acagcaactt atactcaaac tataactgat gcagatatta aaagcttcgc aggtatatct
     7081 ggggataata atccagtaca tatgagtgac gaatatgcag aaggttcaag gtttaaaaag
     7141 cgtatagctc atggtctgat atctgctagt tttttttcag ctctatttgg tacaaaatta
     7201 cctgggcctg gatgtgtata tgtaaatcaa agcttgaaat ttcttagacc tgtttatatt
     7261 aatgatacgg ttacagcgcg tgtagtctta acagacattg atgttgtaaa aagaagactc
     7321 ttttttgata cgatttgtga agttaaccga aaaaaagtta tcacggggaa ggctgaaatt
     7381 tacttgccag aataaagcgc tttaaatttg atgttaactt gctacatatt gatgttttat
     7441 ctctttttat ctttgaagtt tatcaaaaat gaatgtttcc atgtcagata taaaagttag
     7501 tgtatgtatt atttctttta accaacaaaa ctatatacga cagtgcttgg atggtgtctt
     7561 ttctcagaaa acaaattttg agtatgaggt tattatacgt gatgattgca gtaccgataa
     7621 tacatattta acaataatgg aatatattga cactttagat gaagagaaaa aaaagaacat
     7681 aaaaataacg gtacttgatg gcacgaaaaa tattggagca aataataatt ttatcgaaac
     7741 atttaagact tcagtaggtc aatggctagc tatatgtgag ggagatgatt attggtgtga
     7801 tcaggggaaa ttacaaaaac agtacgacta cgctatttca catagtgatt gctcattagt
     7861 cgtgcatccc gctctgataa gtgaaaataa tgtaatacgt aaaacatctt gggcgtgtat
     7921 gaataaaaca ataaatcagc ttagcgatgt aatacgtgca aaaggacagt tctcaccaac
     7981 tggctcctat tttttcaaac gagaaatctt aaatgttcta ccattgtggt tttcaacagc
     8041 tcctgttggt gactattaca tggaaatctt tgctacatcg cttggatctt gtcatacaat
     8101 tcctgatgct atgtcagttt atagaatcaa ttcaacgggg tcttggtctg acctgttaaa
     8161 aaaagataga aatggccaac ggattattaa tacttatctt tctcaattgg agtatttgga
     8221 taaacttgcc gaaatctttc cttcttgtgt cgatgatatc acaataaaaa gatcgcatgc
     8281 acaatatgca gcagcaatgg gttatcttgc aaatggtgat tatactaatt ttcgtatatt
     8341 aatggataag tcagatacaa acggctggta tgataatatg cattctattt tttattattt
     8401 aagaaatagc agggcgcttt ccatgttaat gttcagattc aagcctgcaa ttaaatctgc
     8461 tgtcatgtgg gggcgtcgtg tatttaacaa ctaattattg cgactttttg tcccgaactc
     8521 gaatcgttag gcgcatattt ttgttagttt atttgatttc aaatgtttac gcctatatag
     8581 tgtttactga aacaggagta ttaatagggg attatcaagg agtactcata atatataaag
     8641 atcaggttgt gtatttattg atgttaacaa taatgtcata ttatattgtt tgtggtcctg
     8701 tttttaactg tctatcaaaa gtaaaaatta gactaaggac atttaaaggc ttaaactctt
     8761 ttgcttatat cctgtttttt tttcaaatcc tatttgctgt ttttaatatt tcaacaggtt
     8821 cgaatgccgc gggcactgat tttcaaactg gtggagtcat aagactatta tggctttttt
     8881 taccagttga ctatctattt tatatatatt atttcgtagg ccgagagaaa aaagtaagta
     8941 aaatatatct ggccaatgtg gttattttta ttctatcaat gttaagtaga ggatggcttg
     9001 ggtggacttt ggttttgctt tatgcagagt tatgtttctt tttttattca caaaaaaaaa
     9061 ttaaaataaa atatcttatc ttattgtttt tcttgcttat tgttgcacca ttagcattta
     9121 gtctaaaaat tcaattgcgt gctgatttgt attccagtgg tattggtggg gttatatcaa
     9181 ctttaagtaa tattgattac attcaatctt ataataattt catcgctggt ttcttatcca
     9241 gaatacaaca gttatcaaat attgttttct tttatgacca tcaacaagaa ttatataaat
     9301 ttgtttcttc agatattgta tcaaactatg cttgggaagg attacctcaa caaactgtag
     9361 ctaaacttct aggattagat cctggtgttg atatgcatat ttttctttat agtcattata
     9421 tttcttcaac ttcagaggct gtaactacat tgcaagttgg attcatttct tggctctttt
     9481 taggcaccct gtcatccgta ttttatccac tgtttgtctt tgctattata tgcatatcct
     9541 tatttttatc taaaaaactt ggaggggaaa aactatgtgc acttacgtgg attatgattt
     9601 ttctttctat tatgtgcggg tggtataatg cttatttggt atatatgcag gcattaatta
     9661 cattttattt tatcatgggt tttttgaatt taattactct agaaaaaaca aaaattaatc
     9721 atacatgatg gatgacgaga aatatatgag taatttagta atagttaacg caacagcttt
     9781 agcgtctagc ggcgctttga cgattttgaa ccagttttta gaacacgcac taaatgattc
     9841 taagcataag tatctttgtt ttatacatga gagtgttaaa aaaaagagtg ttaataatgt
     9901 caccgttatt actataaaaa aacaaagttt tattcaacga gtatggtggg atctatatgg
     9961 attgaataaa tatataaaga aaaataattt gaaacctaaa aaaatagttt ccttgcagaa
    10021 tacatctgta aatagtaatt tcgagcagat aatatatcta caccagtcga ttccctttag
    10081 caattttaga ataaaaatta aatttcaata tgctatattt ttcatgtata aatacgtgta
    10141 tccttttttt atattcttta gaactaaaaa caccactttc gttgttcaga ccgattggat
    10201 gaaagatgct atcgtagcaa agaaaaaaat tgccaaagaa cgagttcatg taattaaacc
    10261 tgatataata ttaccggtta ataatcttgc ttaccctgat gaacatgaca aagataattc
    10321 atctgaagtg ttttttttat atccggccac gccacttttc tataaaaacc atttgattat
    10381 cttagatgca atgaggattc taaaaacaga aggaatactt gctaatacaa aattccaagt
    10441 gacttttaaa caagatgata atgatgagtt agcaattaaa atagccaaat atgatttagt
    10501 tgataatata agcttcttgg gagtattatc ctatgaagaa ttgtttcata aatacttaaa
    10561 agcagatgct atactttttc ctagctactt ggaaagcttt ggtttaccgt tagcagaggg
    10621 cgcaatgctt gggaaatata taatttgtag cgatttgcca tatgcaagag atgtgcttaa
    10681 caattattca aatgtagaat atataaatca tgatgatgcc agtaaatggg cattagctat
    10741 gaaggaaata atattaaaga atagaaataa tactttatct gaaagtgaaa atagctatat
    10801 gtattgtccg aaaacaagct gggcagattt cttcgagtta atctaattga ggtatacgat
    10861 gtttaaaaat aaggtacttc tcattactgg tggcacaggt tcctttggga atgccgtttt
    10921 acaacgcttt ttagattcag atattggcga aattcgaatc tttagtcgtg atgagaaaaa
    10981 acaagatgat atgcgtaaaa aatacgcaag tgataagtta aaattttata taggggatgt
    11041 gagggactat agcagtgttt taacagcgac tcgtggggtt gattttattt atcatgcagc
    11101 agcattaaag caagtacctt catgtgagtt ttatccaatg gaagctgtca agactaacgt
    11161 tattggaact gataacgtac ttgaagctgc aattgcaaac aaagtttcac gtattgtttg
    11221 tttgagtacc gataaagctg tttatccaat taatgctatg ggtacatcta aggctatgat
    11281 ggaaaaagtt atcgtagcta agtcaagaaa cttacctaaa gatattacta tttgtgcgac
    11341 gcgatatgga aatgtaatgg cttcccgtgg ttcggtcatc cctctgttta ttaatcaaat
    11401 acttgaagga cgcccaatca ctatcaccga tccgtgtatg actcgattca tgatgacttt
    11461 ggatgatgct gtagatcttg tattacatgc ttttgaacat ggtactaatg gtgatatttt
    11521 tgttcaaaag gcaccagctg caacaataga tacacttacc aagtcattac ttaagttaac
    11581 taaacaacat tctcacccaa ttaatatcat tggcactcgc catggtgaaa aactatttga
    11641 ggtactctgc agtcgggaag aaatgttggt tgcagaagat caaggtaatt actatcgtat
    11701 acctagtgat aaacgtgatc taaattatga aaaattcttc gacaaaggga ctaaagaaat
    11761 tcagtttgtt gaagactata attctcataa tacccgtcgt ttagatgttg atgaaatggt
    11821 tgctcttctt agaaaattag attacatcaa taaaattgag gctggtgaaa aggcagatcc
    11881 cgatgcgtaa aaaaattcta attgttggcg caaatggcat gttaggtagt agtttactac
    11941 gctacttttc atcaattgga gattatgaag ttcttggtac gacaagaagt atggtcgttg
    12001 caaaacaact tgagcaaaag cacaatgtga aaattattga caacgttgat gttattgatt
    12061 ttaaacgatt agagactgta gtagtagagc ataagccaaa tattgttttc aactgcgttg
    12121 ggataattaa acaacttgat gcagcaaaaa acaatatatt atctattgaa attaactcat
    12181 tacttccaca taaattagct caattatgtt cagctcatag tgctaaactc atacattttt
    12241 caacagattg catttttaaa ggtaccaaag gtaattatgt tgaggatgat gagtctgatg
    12301 caattgattt atatggtaaa tctaagttct tgggtgaagt tgaatataat gggcatttaa
    12361 ctttgcggac ttctattatt ggccatgagt tgggatcaaa tcatagtctt gttgactggt
    12421 ttttatcaca gaaaaaatcg gtgaaaggat ttactaatgc gatattctca ggcctcccaa
    12481 cttgttatat ggcagaggtt atccataaat atgttcttcc caacaatctt gctggtttat
    12541 ttcatttaag tgtagagcca attagtaaat atgatttgtt aaatattatt aaaatagtat
    12601 atggagtaag tactgatata gagccaacca acgaatttaa aatagaccgc agcttgaatt
    12661 ctacgctatt tcgtaataag acaaattttg tcccagaatc ttgggataaa ttaattgaaa
    12721 agatgaaaga tgaatacaat aaatatttct aattcaaaaa tacgggttgt tactgtagtt
    12781 ggcactaggc ctgaaatcat tcgtttatcg agggtaattg ctgttctcga tgagtacact
    12841 gaacattttc ttgttcatac cggacaaaac tatgattacg agcttaatga ggtattcttt
    12901 aatgagcttg aaatcagaaa acctgatttt ttcatgaatg ccgcgggcca gaatgctgct
    12961 gagaccatcg gaaatgtaat tattgaagct gataagatat ttgataaatt gcaacctgaa
    13021 gctttactta ttcttggtga tacaaatagt gctctagttt ccattgcagc taaaagacga
    13081 aaaattccta tctttcatat ggaagcgggc aaccgatgct ttgattacag agttccggag
    13141 gaaataaata ggaagattgt tgatcatatt tcagatatta atttaacata tagtgagata
    13201 gctagggatt atcttttgag ggaagggctt cctgctgatc aaataatcaa aaccggtagc
    13261 cctatgcgag aggttctaaa tttttataag gataaaattt catcttcttc tattcttgaa
    13321 aaacttaacc ttcaagcatc tagttatttc cttgttagta gtcatcgaga agagaatgtt
    13381 gattcgcctg agaagttgcg ctctcttatt gagacgttaa atatagtatc tgagaaatat
    13441 aaactgccag taatagtatc aacgcatcct cgtactcgta atagaattga tgctttaggt
    13501 atcacggtaa gtaataatat cattttttct aagccattcg gatttttaga ttatataaaa
    13561 ctgcagcaaa atgctcgtgt cgtgctctct gatagtggaa ctataactga agaatcatca
    13621 gttttgaatt ttccagcctt aaacctgcga gaagtacatg agagaccaga aggatttgaa
    13681 gaagctgttg ttatgtttgt cgggctagat agaaacagaa ttattcaggg aatcgatatt
    13741 ttgcagggtc aaaaacgtgg tgataatgat cgtgaccttc atatggttac agattatcag
    13801 gcagacaatg tttcaattaa aattcttaga attattatga gctatacaaa ctttattaat
    13861 caaaaggttt ggaaaaaatt ctaatgcgta ttgcgttaat atgtgatgac tatttgcctg
    13921 atagtactcg tgttagtgct aagatgatgc atgaattggc ttgtgaactt ttagaaaaag
    13981 gacatgagcc aatcgttatt tgtccttgca ataaaataca gacgctcgaa attttgaatt
    14041 tggatggtgt cgtcgtttat aagttcccta atggtgctat aaagaatgta tctaaaatat
    14101 caagagcaat aaacgaatcc atgctatcat tcaacgcatg gcgatttatt ggaaaatata
    14161 ttcgagaaac taaaattgat ggtgttgtgt actactcacc atctattttt tttggaaaat
    14221 tagcaaataa aatcaaagaa aattggcatt gtaaatcata tttaatctta agggattctt
    14281 ttcctcagtg gctggtagat caggggataa ttaaggaggg agggcttgcg gaacgatatt
    14341 ttaggtactt cgagcagatc aattatgatg ctgcagatta tattggttta atgtcggata
    14401 gaaacaaaga tattttcatt aataaatatc agaataaata caaggtgcaa actttattca
    14461 attgggctga ctttaaaggt atagataata ttcccagtgc gactctacga tcaaaattag
    14521 cacttcaaaa caaagtaatt tttttttatg gaggcaatat tggtcatgct caggatatga
    14581 tgaatttaat gaggttggtt agatcagcat cttatcgtga tgatgtgcat tttttattga
    14641 ttggacaagg tgatgaagtt tcccttgtaa agcagtttat tatagataat tctttaaaaa
    14701 actgtacgta tttaccatcc ataacacaat cagagtttaa gtctgtatta aagattgttg
    14761 atgttggttt gtttagcctt gctaaaaatc atactgttca taacttccct ggtaaattgt
    14821 tagggtatat ggcaaataaa ctccctatat taggtagtgt taatgcaagc aatgatgtta
    14881 tggagattat caatggggca aaggccgggt ttgtttttgt taacggaaat gacgaggctt
    14941 tacttaatgc tgcaataaat cttgcggatg atactcagct aagaaaaaac ctgggatgta
    15001 atgcttactc tttattacaa gaaaaattct ctgtggaaat ggctgctgaa aagatattaa
    15061 gcagcttatt ttcctaacag taatatggtg gttttatgaa agtgtctttt gtaagtcagg
    15121 atgatttcga gaatttaatt caggaagcat ttgcatccgt tcgcttgaga tctcacttac
    15181 tcctccatga aagccctaat gatgctgtcc agagaataat gataggactt gtcaaaggaa
    15241 cgtatatccc gcctcatttt catgaatttc agcatcaatg ggaacatttc catgtgtttc
    15301 aaggtgaggt tgaattaatg ctatttgata gtaacggctg tttaaataaa aaggttattc
    15361 ttggtgggca gagtaagaat attattgcac aaatttcacc actcacccca catacactag
    15421 tttgtagatc tcctacagca gttatcatgg agattaaaga aggtcctttt gatgaaaaat
    15481 gcgctaaagt catcccctca tggtcatata gtgaagatta ttccatttta tccagagata
    15541 ggatcatcgc aatgatgggc caattatcaa ttggagatag atttagtctc taaacttcac
    15601 agcaattagc gaagtaatct tctttttcat accttctata ttatatttat ccactactat
    15661 tatagctttg aatactgagt taacatctac actacattca agccgtgcat acgtcgcggt
    15721 gaccacccct gacaggagta aataatgtca aagcaacaga ttggcgtagt cggtatggca
    15781 gtg
//
