LOCUS       EU296419               10311 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O124 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296419
VERSION     EU296419.1  GI:187880627
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O124"
     gene            94..1608
                     /gene="wzx"
     CDS             94..1608
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37133.1"
                     /db_xref="GI:187880628"
                     /translation="MSRNKIIIKNTMFLATRTLVSLSVSFYTTRIVIQQLGAADYGLF
                     NIIYGVVTFFTFVVTAMNDSVQRYIAIGVGSQKISVIRDAVKNSMFIFVISAFILAVC
                     LLLARGVIIHNILNIPKDSIENASVLYLVAVFSITILIIQTPLNAMVLAYEKMSFYAY
                     MMIFEMVAKMSMALLLTLLEKDKVIVYSILLGSISFFNLLVYLCYCLICFKKSMFGGR
                     IKFRVLKEISTFSFWNIFGNFSYMCRVQGVNIVINMFYAIAVNAAYAISITVLNAINT
                     LTQSLITALRPQIFKSYGECDLKRYNHLVLFGSKYTFSILFLLSSPVILCADELLKIW
                     LDIVPDYTVEFVRLVIVVAFIDSFSYSMIAGIQATGRIKTYQLVVSLIVLINLPLTFI
                     LFKAGNNVLSMFYPFIVTAIINQGLRLYFIYINAGFDYKKYFTVVIYPCLLAVCLSLV
                     TDISIKKMLPFNSVIDVLIVCIFIFSFNTIIFYWVVVSKKEKKWLLETLKRKVK"
     misc_feature    94..10191
                     /note="O-antigen gene cluster"
     gene            1572..2954
                     /gene="wfeO"
     CDS             1572..2954
                     /gene="wfeO"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeO"
                     /protein_id="ACD37134.1"
                     /db_xref="GI:187880629"
                     /translation="MVIRNPEKKSEITIKDVIDNELCTGCGVCISEDSSKTSFMKWNS
                     EGFYEPCFSPVSTLFNMQRVCPFNLSRDTLVNEDELAHEFFDGKGYLDSEVGFYKKIY
                     VGYSKHFRETSSSGGIATYVFEQLLRRKYVDALFIVRELGGSYGYQVFDNPEHIKDMS
                     KTRYYPVTLEKLFDNIYKLNGRVAVSGVACFIKAIRLKQHYHPELKEKIPFLVGIICG
                     GLKSRYYTDYLSQSAGCVSEYQNAEYRVKKKDSHALDYRFTCVEKSNNIIHSVDMQRM
                     GDMWGSGLFKANACDYCDDVTTELADISLGDAWISPYNMDGAGNNVVVCRSTTAHEII
                     LSGIEKKDLELTELELEQLKLSQQGSFNHRHKGLLYRIKNAEKNNRLVPVKRKRFLRS
                     ISFLLKLIQKQRSVTRRKSIEIWMETQNSATFDKKMKGYLFTLRWLTVVNRKLSRMFK
                     IVSLNRMGKK"
     gene            2951..4087
                     /gene="wfeP"
     CDS             2951..4087
                     /gene="wfeP"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeP"
                     /protein_id="ACD37135.1"
                     /db_xref="GI:187880630"
                     /translation="MKVAILTQPLHTNYGGTLQAYALQKVLINLGHEPETINYRSKIK
                     RPLFIRVVLSKIKRIVLCRKITFDFTTQDRINIRKHHQSFIDTRLNYSEEINGTEGLR
                     DYILKNNYGAVIIGSDQTWRPIYSPRIDSFFLDFLSDVNDIKKMAYAASFGTDKWEFT
                     ESQTNLFKLLLSKFDYVSVRESSGVKLCSEKFGLKAELVLDPTLLLTFEDYTHLLDND
                     YINLHKGKVFSYVLDENYDKKNFIDTVASTLRTTSFYTYPKKITKDEYVIRNYSEYEY
                     PPIEYWISSFKAAEFIVTDSFHGTVFSIIFNKPFIAIANEERGKARFTSLLEMFGLER
                     RLVSNLDDVDLKLVNEKINYGPINEKIAYFREECLKKLQLMLIK"
     gene            4134..5138
                     /gene="wfeQ"
     CDS             4134..5138
                     /gene="wfeQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeQ"
                     /protein_id="ACD37136.1"
                     /db_xref="GI:187880631"
                     /translation="MKNCKVSVIIPVYNAEKYIQRCILSLLKQTLDDVEIIIIDDGST
                     DNSLSIIKETVALHTASRARQKRINIISRENKGVAYTRSQGLRLSQGEFIIHFDSDDW
                     AKSNMLEEMYKTIVSNNADMVICDYFLVKNNKEILIKQRVEYEPKKCIRYLLTGELEG
                     FTWNKLIRKKYIDKNKIDFVNKITYMEDFLFILSVLLHNPKIIFQDCAYYYYQKSNPK
                     SLTSHASVDRLSEMIKAVSEIEKKINKYNLQKYLNNEFQLFKLKQKIWFISISKLNVN
                     DNVWNLFPETNPFISKVNVLFYYKIVLFLDSIKLRFFSNKIIYLIGLVQALLQERKEK
                     "
     gene            5135..5923
                     /gene="wfeR"
     CDS             5135..5923
                     /gene="wfeR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeR"
                     /protein_id="ACD37137.1"
                     /db_xref="GI:187880632"
                     /translation="MIPKIIHYCWFGRSPLSELTKQCIASWEKYCPEYKIIRWDENNV
                     DLNSCSFVRQAYKEKKWAFVSDYVRLKVVNEYGGIYLDTDVELIKPLDDLLIYPAYIG
                     FEINKEWYVNSGLGFGSVNNNPVLESLIMEYENINFVNKDGTLNITPCPIRETKALTK
                     IGLIPDGQCQSFDNIVIFSADYFCPVSITGERNFSDKTYSIHHYDASWFSEQKRKGLQ
                     RKKRFIKLFGNLIGTYINKPFIFVDECREFGLVKAIKNMRSNFP"
     gene            5997..7100
                     /gene="wzy"
     CDS             5997..7100
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37138.1"
                     /db_xref="GI:187880633"
                     /translation="MYFLLFFSIFYFVYVILIGTLCHNYKALLIFSLIPLTIVSGIRY
                     NVGFDFMSYVDYFNQLKYSDDIYLDSTFKYISYFTYYIGGNEQILFLIYAVFYSVALY
                     FLIKLVLENYITEINNFYTIGLVLSFYSFYFLLSFNQIRAVLSALILCYGLLKNKIDF
                     SFIITITLSILFHSAAMFILPLYFVLRKINVTVLLVIFPFLVVASFFNIFSDIVRFIL
                     TLLNSRFLTYFNSEYFVPRTGMEKMYSMISMIIVLGMVVCLSKLLPKKFDLMIKFVIL
                     FVLLRAMSIDILIFARFSDFLKPMAIILIFTAVYFSSNKVRPRIILPFYLIMTLFLCI
                     FNIIIGSNITKGNYYTYGYNICFFGNKCIDKFY"
     gene            7129..8178
                     /gene="wfeS"
     CDS             7129..8178
                     /gene="wfeS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeS"
                     /protein_id="ACD37139.1"
                     /db_xref="GI:187880634"
                     /translation="MEKYMNNFSCKEPPSFIENKIILSIILPVYNVSEYLIECLNSLL
                     LDVHERYLNRCEVIVVDDGSTDNSFELMREYSLKYPESIKIYSKFNGGLSDARNYGLL
                     KSSGKYISFVDSDDVVNRGFIKEIINFIDAYDFDILSFDFMKFFNNNDALILSQIDEF
                     SKMAERVDSEFYKSKPVFAWNKVYRRSLFDNEVFPKGWYYEDVALIPLLLDRAKVLYH
                     INSVCYFYRQRQGAITFFYDNKYLDILKGVSFLYDRSQSSFIKTIIINQFFTLTLLSL
                     RLPTDNYFMNMRGIIDTYCEKFDLDSFEPKLILKHIPFLLLKKLKSLCVYPLYLFKPA
                     VFLHKQIKHFRGKLK"
     gene            8175..9284
                     /gene="glf"
     CDS             8175..9284
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37140.1"
                     /db_xref="GI:187880635"
                     /translation="MKYDYIIIGSGLFGSVFAHELGKMKKKVKVIERRDHVGGNVYCE
                     NIEGINVHKYGAHIFHTNDKEIWDYVNQFVEFNRYTNSPLAFSKGKIYNLPFNMNTFN
                     QLWGAITPQHAKEIIKKQSGEISGNKPRNLEEQAISLVGRDIYNCLIKEYTEKQWGRP
                     CTELPSFIIKRLPVRFTYDNNYFNDQYQGIPVGGYNKLIDALLNGIEVQTCTDYFEEK
                     KYWDSIAKKIIYTGPIDKFYCYKFGALDYRSLKFEHELLNVDNFQGNAVVNYIDKKFP
                     FTRIIEHKFFEFGEQHNTLITREYPKEFEEGDEYYYPVNDEKNMQIYKMYYNLSKQES
                     NVIFGGRLAEYKYYDMHQVIKSALNAFRKEVRLGE"
     gene            9277..10191
                     /gene="wfeT"
     CDS             9277..10191
                     /gene="wfeT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeT"
                     /protein_id="ACD37141.1"
                     /db_xref="GI:187880636"
                     /translation="MNKEVIAVIVTYNRKALLLKVIDAVINQSYPLKKILIIDNNSTD
                     GTEMFISNRLSDVVKYKNTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDFMPTVNCL
                     EILISNSPDGIVQPVRYNLDETCAELSPLTYDLSNPFKLNPKGTPLKNYLNTIGNKSA
                     KVDIEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRKGIPIQCNLKAKAY
                     RLLVNNQGNDLLSWKGYFMLRNLFYLHKTYGTNFLVRQKPIVLGLGYALSCVLKCNFS
                     QLPVIWRAFWDSSTLRNTEKFRPEMRSK"
BASE COUNT     3488 a   1302 c   1842 g   3679 t
ORIGIN
        1 gattattggt agctgtaagc caagggcggt agcgtgcatt aatattcaca atatattctt
       61 ttaaatgtaa gcagaattaa tcgaacctaa ataatgtcac gtaacaaaat aatcataaag
      121 aatactatgt ttttggcaac aagaacctta gtttctttaa gtgtttcatt ttacacaaca
      181 agaatagtta tacaacagtt gggggcggca gactatggct tatttaatat catttatgga
      241 gttgtaacat tttttacatt cgtagtcacc gcgatgaatg attctgtaca gcgttatatt
      301 gcaataggtg ttggttcaca aaaaatatcg gttattagag atgctgtaaa aaatagtatg
      361 tttatatttg ttatatctgc tttcatattg gcagtatgtc ttcttttggc aaggggggtg
      421 atcatacata atattttaaa tatacctaaa gattctattg aaaatgcaag tgtgttatat
      481 ttggttgcag tattttcaat aaccatactt ataattcaga cgccacttaa tgcgatggtg
      541 ttagcatacg agaaaatgtc attttacgca tacatgatga tatttgaaat ggtagctaaa
      601 atgagtatgg ctttgttact aactttactg gaaaaagata aggtcattgt atattcaata
      661 cttttaggtt ctatttcttt ttttaacttg ttggtttatt tatgctactg cttgatatgt
      721 tttaaaaaat caatgtttgg tgggagaata aaatttagag tgctgaaaga aatttcaact
      781 ttttcatttt ggaatatatt tggtaatttc tcttacatgt gcagagttca gggagtgaac
      841 attgtaatta atatgtttta cgctatagcc gtgaatgcag catatgcgat ttcgattact
      901 gttttaaatg caattaatac acttacgcaa tcattaatta cagcattaag gcctcaaata
      961 tttaaatcat atggcgaatg tgatttaaaa agatataatc atctagttct ctttggttca
     1021 aaatatacat tttcaatatt gtttctgtta agtagccctg taattctatg tgcggatgag
     1081 ttgttaaaga tatggcttga tattgtacca gattatacag ttgaatttgt gaggttggtc
     1141 attgttgtgg cttttattga tagtttttca tatagcatga ttgctggtat tcaggctacg
     1201 ggtaggatta aaacatatca actagttgtt agtttaattg tcctaattaa tttgccgtta
     1261 acctttattt tgtttaaggc aggaaataat gttttaagta tgttttatcc atttattgtt
     1321 actgccatta tcaatcaagg tttaaggttg tattttatct atatcaatgc aggttttgat
     1381 tataagaagt atttcactgt tgtgatctat ccttgcttgt tagctgtatg cttatcgtta
     1441 gtgacagata tttcaattaa aaaaatgctt ccatttaaca gtgttattga tgttttaatt
     1501 gtgtgtattt ttattttttc attcaataca ataatttttt attgggttgt agtttctaaa
     1561 aaggaaaaaa aatggttatt agaaaccctg aaaagaaaag tgaaataaca ataaaagatg
     1621 ttatagacaa tgaactctgt actggttgtg gagtttgtat ttcggaagat agtagcaaaa
     1681 cctcctttat gaaatggaat tctgaaggtt tttatgagcc atgtttctct cctgtatcaa
     1741 cattattcaa tatgcagcgt gtttgtccct ttaacttaag tagggataca ttagtgaatg
     1801 aggatgaact tgcgcatgag ttttttgatg gtaagggata tttagattct gaggttgggt
     1861 tttataaaaa aatatatgtg ggttattcta aacattttag agagacttca tcctccggtg
     1921 gtatagctac atatgtattt gaacaacttc ttagacgtaa gtatgttgat gctctattta
     1981 tagttaggga gcttggtggt tcatatggat atcaagtctt cgataatcca gaacatatta
     2041 aggatatgtc taaaacaaga tattatcctg taacgctaga aaagttattt gataatattt
     2101 ataagcttaa tggtagagtg gccgtttcag gagtggcatg ttttattaaa gccattaggc
     2161 tcaaacaaca ttaccatcca gaactgaaag agaaaatacc attccttgtt ggtataattt
     2221 gtggaggact caaaagtcga tattacactg attatttatc acagtctgct ggttgtgtca
     2281 gtgagtacca aaatgctgag tatcgagtga aaaagaaaga tagtcatgct cttgactatc
     2341 gtttcacatg tgttgagaaa tcaaataata taattcattc tgttgatatg caaagaatgg
     2401 gggatatgtg gggttcaggg ctatttaagg caaacgcatg tgactattgt gatgatgtta
     2461 caaccgaact cgcagacata tcattgggtg atgcctggat ttcgccatac aatatggatg
     2521 gagctggtaa taatgtagtt gtatgtcgct ccaccacagc tcatgaaatt attttatctg
     2581 ggattgagaa aaaagactta gaattaacgg aactcgaatt agagcagttg aaattgtcac
     2641 aacaaggaag ttttaaccac aggcataaag gattattata tagaataaaa aatgcagaga
     2701 aaaataatcg cttagtgcct gttaagagga aaagattctt acgctctata tcttttttat
     2761 taaagttaat tcaaaaacaa cgatcagtta caaggagaaa aagcatagag atatggatgg
     2821 aaactcaaaa ttcagcaact ttcgataaga aaatgaaggg atatttattc acgttgcgtt
     2881 ggttaacagt ggtcaatagg aaattaagtc ggatgttcaa gattgtttct ctaaatagaa
     2941 tgggtaaaaa atgaaagttg caatattaac tcaaccatta catacgaatt atggtggcac
     3001 attacaagcc tatgcattac aaaaagtctt gattaatttg ggacatgaac cagagactat
     3061 taattatagg tcgaaaatta agaggccact atttatccgt gttgtgttgt caaaaattaa
     3121 aaggatagta ttatgccgaa aaatcacatt tgattttaca acacaagata gaattaatat
     3181 taggaagcat catcaatctt tcattgatac aagattaaac tattcagagg agattaacgg
     3241 tactgagggg ttgagagatt atattttaaa aaataattac ggtgctgtaa taataggtag
     3301 tgatcagaca tggagaccaa tatattcgcc tagaatagat tcattttttc tcgatttttt
     3361 aagtgatgta aacgatataa aaaaaatggc atatgcagca tcttttggta ctgataaatg
     3421 ggagttcacg gagtcacaaa ctaatttatt taaattgtta ctttcaaaat ttgactatgt
     3481 ttcagttaga gagtcctcag gagtcaaatt atgtagtgaa aaatttggtt tgaaagcaga
     3541 gttggtttta gatccgacat tgctattgac attcgaagat tatactcatt tattagacaa
     3601 tgactatatt aatcttcata agggaaaggt gtttagttat gtacttgatg agaattacga
     3661 caaaaaaaac tttatagaca cagtagcaag tactctccgc acaacgtcat tttatactta
     3721 ccctaaaaaa atcactaaag atgaatatgt gattagaaat tattccgagt atgaatatcc
     3781 gccaatagaa tattggattt cttcgtttaa ggccgctgaa tttattgtaa cagattcatt
     3841 tcatggaaca gtattttcta ttatttttaa caaacccttc atcgccattg caaatgaaga
     3901 gagaggaaag gctagattta cgtcactatt ggaaatgttt ggtcttgaaa gacgacttgt
     3961 gagtaattta gatgatgttg atttaaagct tgtaaatgaa aaaattaact atggccccat
     4021 aaatgaaaaa attgcttatt ttagagaaga gtgccttaaa aagttgcagt taatgctaat
     4081 aaaataatca gattatgtat gtctctctac aattaaaata tgaatgttga tcgatgaaga
     4141 attgtaaagt atctgtaatt attccggtct ataatgcgga aaaatatata caacgttgta
     4201 ttttaagctt attaaagcag acattagatg atgttgaaat tatcatcatt gatgatggtt
     4261 ctactgataa ttcactttct ataataaaag aaactgttgc attgcatact gctagtagag
     4321 ctcgccaaaa gagaatcaat ataatctctc gagaaaacaa aggagttgcc tatacacgaa
     4381 gtcagggatt gagactttcg cagggggaat ttatcattca ttttgatagt gatgattggg
     4441 caaaatccaa tatgctggag gaaatgtata agacaattgt ttccaataat gcagacatgg
     4501 ttatatgcga ttatttttta gtgaagaata ataaagaaat acttataaaa cagagagtgg
     4561 agtatgagcc aaagaaatgt attagatatc tactgactgg tgagctagaa gggtttacat
     4621 ggaacaaact aattagaaaa aaatatatcg ataaaaataa aattgatttt gttaataaaa
     4681 ttacttatat ggaggatttt ttgttcatat taagtgtgtt actacacaat cctaaaatta
     4741 tttttcagga ttgtgcatat tattattatc aaaaaagcaa tcctaaatct ctaactagcc
     4801 atgctagtgt ggataggctt tctgaaatga ttaaagccgt aagtgaaata gaaaagaaga
     4861 taaataaata taatttacaa aagtatctga ataacgagtt tcaattgttt aagttaaaac
     4921 aaaaaatttg gtttatatct ataagtaagt taaatgttaa tgataatgta tggaatctct
     4981 ttcctgaaac aaatccattt atatcaaagg taaatgtgct tttttattat aaaatagtgc
     5041 tttttcttga tagtattaaa ctgcgttttt tttcaaataa gatcatttat ctcattggat
     5101 tggtgcaggc tttattacaa gaaaggaaag agaagtgata ccaaaaataa ttcattactg
     5161 ctggttcggt cgctctccat tgtcggaatt aacaaagcag tgcattgcaa gctgggagaa
     5221 atattgtcct gaatataaaa tcataagatg ggatgaaaat aatgtcgatt tgaattcttg
     5281 ttcttttgtt agacaagctt ataaagaaaa aaaatgggcg ttcgtttctg attatgtaag
     5341 gcttaaagtg gttaatgaat atggcggtat ctatcttgat acagatgttg aattaataaa
     5401 gccattagat gatttgttaa tatatcctgc atatataggc tttgaaatta acaaggaatg
     5461 gtatgtaaat tctggtttgg ggttcggttc tgttaataat aatccggttt tagaatcatt
     5521 aattatggag tatgaaaata ttaattttgt aaataaggat gggactctaa atataacgcc
     5581 gtgccccata agagaaacaa aagcattaac aaaaataggt ttaattcctg atggtcaatg
     5641 tcaatctttt gataatatag tgattttttc tgcagattat ttttgtcctg tatctataac
     5701 aggtgagaga aatttttctg ataaaacata ttcaatacat cattacgatg ctagttggtt
     5761 ttctgagcaa aagagaaaag gcctgcaacg taaaaagaga tttataaaac tattcggcaa
     5821 cctaataggc acatatatta ataaaccttt tatttttgta gatgaatgtc gcgagttcgg
     5881 attagttaaa gcaatcaaga atatgcgttc caattttcct taatttttag tgttaaggaa
     5941 taagtttcgt taaaatactt tattcattta attaataaag gtttaatggt gattagatgt
     6001 actttctttt gttcttctca atattttatt ttgtatacgt aatcttaata ggaacattat
     6061 gtcataacta taaagccctt ttgatttttt cactaatccc tctgacaata gtgtcaggta
     6121 taagatataa tgtcggcttt gattttatgt catatgttga ttacttcaat caactcaaat
     6181 attcagatga tatatatcta gattctacct ttaagtatat aagctacttt acctattata
     6241 taggaggtaa cgaacagatt ttgtttctga tatatgcggt tttttattcg gttgcacttt
     6301 attttttaat aaaactggta ttggaaaatt atataacgga aataaataat ttttatacta
     6361 ttggacttgt gttatcattt tattcgtttt attttttatt atcatttaat caaattaggg
     6421 ctgtattatc agctctaata ctatgttatg ggctattaaa aaataaaata gatttttctt
     6481 ttataataac aattacatta agtattcttt ttcactctgc agcaatgttc attttacctc
     6541 tttattttgt cctaagaaaa ataaatgtaa cagttctgtt ggtgatattc ccttttttgg
     6601 ttgttgcttc attctttaat attttttcag atattgtgag attcattctg acattgctaa
     6661 atagtcgttt tttaacatat tttaactctg aatattttgt tccaaggacg ggaatggaaa
     6721 aaatgtatag tatgatctca atgattattg ttttgggaat ggttgtttgt ttgtctaaat
     6781 tattacctaa aaagtttgat ttaatgataa agtttgttat tctttttgtt ctacttagag
     6841 cgatgtcaat tgatatattg atttttgcta ggtttagtga ttttcttaaa ccaatggcta
     6901 taattcttat atttaccgct gtttattttt cttcgaataa agtgcgacct agaattatcc
     6961 ttccttttta tttaattatg acattgtttc tctgtatttt taatataata atcggatcga
     7021 atataactaa ggggaactat tatacttatg gttataacat ttgctttttt ggcaataaat
     7081 gtatagataa attttattag taattgtcat ttgatacttc tggtatttat ggaaaaatat
     7141 atgaataact tttcttgtaa agaaccccct tcatttattg aaaataaaat tatattatca
     7201 attattcttc ctgtatataa cgtatcagag tatttaattg agtgcctaaa cagtttgctg
     7261 ttagacgttc atgaacgtta tcttaataga tgtgaagtaa ttgtggttga tgatggttca
     7321 actgataatt catttgaatt aatgagagag tattctttga aatatcctga atctattaag
     7381 atttattcga aattcaatgg tggtctctca gatgcaagaa actatggatt attaaaatct
     7441 tcaggtaaat atatttcttt tgttgattca gatgatgttg ttaatagagg ttttataaag
     7501 gaaatcatca atttcattga tgcctatgat tttgatatat tgtcatttga cttcatgaag
     7561 ttttttaata ataatgatgc tcttattctt tcacaaatag atgaattctc caaaatggca
     7621 gaaagagtag attcggagtt ctacaagtcc aagcctgttt ttgcttggaa taaagtttat
     7681 cgacgttctc ttttcgacaa tgaagtattt cctaaggggt ggtattatga agatgttgca
     7741 ttaattccgt tattattaga tagagcaaaa gttttatacc atattaatag cgtttgttat
     7801 ttttatagac agagacaagg agcaatcact tttttttatg acaataaata tcttgatatt
     7861 ttaaaaggag tgtctttttt atatgacagg tcacaatctt cattcattaa aacgataatt
     7921 ataaatcagt tttttacttt aaccttacta tctttaagat tgccgactga taattatttc
     7981 atgaatatga gggggattat tgatacctat tgtgaaaaat ttgacttgga ttcctttgag
     8041 cctaaactaa tattaaaaca cattcctttt ttattgctta aaaaattgaa gtcgctgtgt
     8101 gtttatccac tgtatttatt taaacctgca gtttttttac ataaacaaat taaacatttt
     8161 agaggaaaac taaagtgaaa tatgattata taattatagg tagcggtctt tttgggagtg
     8221 tgtttgcaca tgaattaggg aaaatgaaaa aaaaggtcaa ggttattgag cgtcgtgatc
     8281 atgttggtgg taatgtatat tgtgagaata ttgaaggaat taatgttcat aaatatggcg
     8341 cacatatatt ccatactaac gataaagaaa tatgggatta tgtaaatcaa tttgtagagt
     8401 ttaatagata tacaaattca ccattagctt tttcaaaagg taaaatttat aatttacctt
     8461 ttaacatgaa tacctttaat caactctggg gggctattac tcctcaacat gcgaaagaaa
     8521 ttattaaaaa gcaaagcggt gaaataagtg gaaataaacc aaggaatctt gaagagcaag
     8581 ctatttcttt ggtgggacga gatatttata attgtttgat taaggagtac actgagaaac
     8641 aatggggacg accttgcact gaattgccat catttattat aaaacgactc cctgttaggt
     8701 ttacatacga taataactat tttaatgacc agtatcaagg aataccagtc ggtggttaca
     8761 ataaattaat cgatgcatta ttaaatggta ttgaagttca aacatgcact gactattttg
     8821 aagagaagaa atattgggat tctattgcaa agaaaattat ttatacaggg cctattgata
     8881 agttttattg ctataaattt ggagctttgg attatcgatc attaaaattt gagcatgaat
     8941 tactgaatgt cgataatttt caagggaatg ctgttgtaaa ttatatagac aaaaaattcc
     9001 cattcactcg tataatagaa cataaatttt ttgaatttgg tgaacagcat aatactctca
     9061 ttactcgaga gtacccaaaa gaattcgaag aaggcgatga gtattactat cctgtcaatg
     9121 atgaaaaaaa tatgcaaatc tataaaatgt attataatct ttctaaacag gaaagtaatg
     9181 tgatttttgg tggacgtctt gcggaatata aatattatga catgcatcaa gttatcaaat
     9241 cagctttaaa tgcattccga aaagaggtac gtttaggtga ataaagaagt aatcgcagtt
     9301 attgtaacct ataatagaaa agcattattg ctgaaagtta tagatgctgt tattaaccaa
     9361 agttatccac taaaaaaaat attgataata gataataata gtactgatgg tacggagatg
     9421 tttatttcaa ataggctctc tgacgtggtg aagtataaaa atacggggga taatttaggg
     9481 ggggctggcg ggttttatag aggattcata gaggcggaaa aatatggcta tgattatctt
     9541 tggttaatgg acgatgattt tatgcctaca gtgaattgtc ttgaaatatt aatatcaaat
     9601 agccctgatg gtattgtcca acctgttaga tataacttgg atgaaacttg tgcagagctt
     9661 tctccattaa cttatgattt atctaaccca tttaagttaa accctaaagg tacaccatta
     9721 aaaaattact taaatacaat tggtaataaa tctgcgaagg tagatattga agctattcca
     9781 tttgaaggtc cattgatttc acgaaaggtt gttgaaaaaa taggctatcc agagcctaag
     9841 ttttttattt tttgtgatga cattgaatac gctattaaag ctaaaagaaa ggggattccc
     9901 atccagtgca atcttaaagc caaggcatac cgccttttgg tcaacaatca aggtaatgat
     9961 cttctttcct ggaaaggata ttttatgctt cgaaatcttt tctacctgca taaaacgtat
    10021 gggaccaatt tccttgttag acaaaaacct atcgtattgg gattaggata tgcattgtcg
    10081 tgtgtattga aatgcaattt ttctcaactt cccgttatat ggcgtgcttt ttgggattca
    10141 tctacgttaa gaaataccga aaaattcagg cccgaaatga gaagtaaata atttattatt
    10201 atgtttttat taattaatgt cgatttttac taaaattctt aacacgatat ttatccctga
    10261 caggagtaaa caatgtccaa gcaacagatc ggcgtcgtcg gtatggcagt g
//
