LOCUS       GU068042               11782 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain E611 serogroup O126 O antigen gene cluster,
            complete sequence.
ACCESSION   GU068042
VERSION     GU068042.1  GI:307340782
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E611"
                     /db_xref="taxon:562"
                     /note="serogroup: O126"
     gene            43..1086
                     /gene="wzy"
     CDS             43..1086
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43845.1"
                     /db_xref="GI:307340783"
                     /translation="MYIKIYGFSLMENITIYDYVIVNNGLFNKDAVIVFFIMFYLFGC
                     LINIVSNYVSCIYVLLVLIPLSLLFALNGPDKASYLSLYNAIDTVSDVFKYYNTIDSP
                     LYYITMVLFKNIGLTFYSFMVVQNLIISFSLSYLIYKYKWGYHIAMWLYFTHLLVPFS
                     VRGHLVIITILFANRSVASKIISSLGLVAIHPLISALPITYQLRGLIRGKRSILIIVL
                     TICALVFSASYLISKYTSYAINGDEYVKNTGLSLIYIVDAIELLLFWSIIRPIALIDK
                     LFLLFPVVLSFGTIIIPMLGRFSYACDLVYILILINNANRLKNKKTLFMCLSLIALIR
                     FVKNYYSIEYFIL"
     misc_feature    43..11637
                     /note="O antigen gene cluster"
     gene            1083..2084
                     /gene="wbgK"
     CDS             1083..2084
                     /gene="wbgK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgK"
                     /protein_id="ADN43846.1"
                     /db_xref="GI:307340784"
                     /translation="MMLFFKNPKNKKSTGILYLQTLLHERLSNVRVITSTIMIIKRYF
                     NIMKSTEVAIFLPFTFSCIFAKKNDLVIMPDDSIRSITSVSKVRYENKKILKWLFQNI
                     RLIITKNIISLKKDVVYLTVSKDDQWSLQSKFPKHKIVYIPHPIQSASNKEDVLDRCE
                     NISTICFMNLQEHYSVNPSAFIRPKTKVNLNDLHNKVNIIFHGSYSQAWYGKYMYYYG
                     KANCKAVRYVEDFEQFYNNIDLIILPLAAGAGIKNIVLNSVYRNKLILGTEEAFSGIP
                     KEFTRKLIIDSNDDILTKINSEVECLAIKESCNILRDYLVVEHTEKAFMDTIFKNII"
     gene            2094..2987
                     /gene="wbgL"
     CDS             2094..2987
                     /gene="wbgL"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgL"
                     /protein_id="ADN43847.1"
                     /db_xref="GI:307340785"
                     /translation="MSIIRLQGGLGNQLFQFSFGYALSKINGTPLYFDISHYAENDDH
                     GGYRLNNLQIPEEYLQYYTPKINNIYKFLVRGSRLYPEIFLFLGFCNEFHAYGYDFEY
                     IAQKWKSKKYIGYWQSEHFFHKHILDLKEFFIPKNVSEQANLLAAKILESQSSLSIHI
                     RRGDYIKNKTATLTHGVCSLEYYKKALNKIRDLAMIRDVFIFSDDIFWCKENIETLLS
                     KKYNIYYSEDLSQEEDLWLMSLANHHIIANSSFSWWGAYLGTSASQIVIYPTPWYDIT
                     PKNTYIPIVNHWINVDKHSSC"
     gene            3012..3764
                     /gene="wbsT"
     CDS             3012..3764
                     /gene="wbsT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsT"
                     /protein_id="ADN43848.1"
                     /db_xref="GI:307340786"
                     /translation="MKRPYFSFLLCVNKDEGFLNDALESIINQDYSYEYEIIVIANNC
                     DDNLWAYLNSVESDKLKIHRTNIGQLAFNLNYGANIARGEYIVRMDADDVSTPNRLKI
                     LEAAIQENNYPDVIGTSVYFISETGRIIKKYVPPLGEVKSALIKNPFVHPTVAIKKVS
                     LFKIGGYLGGFQSEDNDLWIRMLRAHEFKLVNIDSVTLFYRINDHQTRGRVLPYAEVA
                     SYALREFLLKKRIKYFAALLINIGKALILPKR"
     gene            3786..4898
                     /gene="wbsU"
     CDS             3786..4898
                     /gene="wbsU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbsU"
                     /protein_id="ADN43849.1"
                     /db_xref="GI:307340787"
                     /translation="MHTVLNTFTAKKFVEPLLDNVNEQHNKEKIIHKILIDPVNGDER
                     FLKELHVHYEIKKFDIDYLFIKHIIELYFYFRKERPDIVIAHMSRGAILPLISAFIAR
                     VPKRIYFNHGVPYIGYKNLPRMCFKLLEKLNCFFSNQVLTVSQGMIEPLTAITKKPVS
                     ILGPGSACGIDHNEFQRKSKYDIENIKQQYAASDTFIFTFVGRPVARKGFDITLKAFT
                     KAFSGADNVILLLAGATSEDIFRVLGSKPNNIKPLGFVMDLDNVYLATDVVVVPSEHE
                     GFCYALLEGAARECALICSDIPGPDAIVINKRNGILITPKNIDSLMVSMSELFNDREM
                     TKKYGEQAYMIASEFERKKIMLYYSYYIRSLFIEQR"
     gene            4971..6092
                     /gene="gmd"
     CDS             4971..6092
                     /gene="gmd"
                     /note="GDP-D-mannose dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ADN43850.1"
                     /db_xref="GI:307340788"
                     /translation="MKKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASLFNTNRI
                     DHIYQDLHHEKQSFFLHYGDLTDSLNIVRLVQEIQPDEIYNLGAQSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRICGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KMYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRALANIAYGLEPCLYL
                     GNMDALRDWGHAKDYVRMQWLMLQQETPDDFVIATGKQISVREFVRMAAANLGIELEF
                     IGCGLKEVGVVKAIHGNEAPSVKIGDVIVQVDPRYFRPAEVETLLGYPEKAKIKLGWE
                     PEITIEEMCKEMIRNDLNIAKQHSLLKLNGYDVSLSVEK"
     gene            6097..7062
                     /gene="fcl"
     CDS             6097..7062
                     /gene="fcl"
                     /note="GDP-L-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ADN43851.1"
                     /db_xref="GI:307340789"
                     /translation="MKMKKIYIAGHRGMVGSAIKRQLMKNSSVELIFRSSHELDLMNQ
                     DAVQNFFNTERIDEVYLAAAKVGGIYANNKYPADFIYNNILIQANVINSAHRAGVQKL
                     LFLGSSCIYPKFAEQPIKEESLLCGSLEPTNEPYAIAKITGIKLCESYNRQYGRDYRS
                     VMPTNLYGPNDNFHHENSHVLPALIRRFHEAKINNLPEVSVWGSGNALREFLHVDDMA
                     AASIYIMNLPNERYKEYTKPECSHINVGSGKDYSIRQLAEMVAEITNYKGNIYFDSTK
                     PDGTLRKLMSNERLSKLGWSPKIELYDGVKDTYQWFLNNIENIRK"
     gene            7148..8539
                     /gene="manC"
     CDS             7148..8539
                     /gene="manC"
                     /note="mannose-1-phosphate guanyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADN43852.1"
                     /db_xref="GI:307340790"
                     /translation="MLFPIIMAGGTGSRLWPLSRESFPKQYLKLYGDMTMLQSTIARL
                     NTLDCSTPLVICNEEHRFIVAEQLKKINKLDKNILLEPQGKNTAPAITLCAFAALKYA
                     KNDEDPLLLVLAADHVIGNISSFTKSVKNALPHAHAGKLVTFGIIPTTPETGYGYIHR
                     GEKFNDAYKVSSFTEKPDIYRAQEFLKTGEYYWNSGMFLFSAKRLLAEMEKYRPDIFS
                     ACEQAFLSPSNDLDFIRVDRDKFSLCPSESIDYAVMENTDDAIVVPMDAEWSDVGSWS
                     SLWDISDKDVNGNAVSGDLISFNSYNNFIKAEDKLVALVGVNDLVLVHTDDAILVSSK
                     YEVQNVKKIVEVLNQNGRDESKKHKEVYRPWGEYHYITEAEKYNVRRVLINPGQKIST
                     QTHNHRSEHWVIVSGIAKITINKNINIIKENESIYIPPGTMHTIENSEMLPLEIIEIQ
                     TGQLLTEDDVIRP"
     gene            8610..10049
                     /gene="manB"
     CDS             8610..10049
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADN43853.1"
                     /db_xref="GI:307340791"
                     /translation="MSKKFNTLSIIRNSGVAFGTSGARGLVTEFTPEVCGAFSHAFIN
                     VMKQKYKFHGVALAIDNRPSSYSIAQACASVIAQYGLKVNYYGVIPTPALAYKALEDK
                     IPCIMVTGSHIPHDRNGLKFYSPIGEITKEDEIRIKDVSIDFTLDSSIPNLYPCPDAA
                     SCYFKRYIDFFPEGILKGKRVGFYEHSSAGRDLYIPIFEKLGAEIVSIGRSDKFIPID
                     TEAVGQDLVIQAKKWALEYKLDAIFSTDGDGDRPMLSDENGKWLRGDILGLLCAETLN
                     IKSIAIPISCNSLIMQRKCFEEVVLTKIGSPYVLDAFSSLKDKYQNYAGFEANGGFIL
                     GSDITVDNRKIKALPTRDALLPILVLLSANKKISSLVHSLPSRFTYSDRIANFLPEKR
                     DRLFTICKNNPHTIVDLLGENEAGIEVKDINNLDGYRFTLNNNVIIHLRSSGNAPELR
                     CYCEADSEFCAQKYVERILSSVACLIKIN"
     gene            10153..11637
                     /gene="wzx"
     CDS             10153..11637
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43854.1"
                     /db_xref="GI:307340792"
                     /translation="MQNNKRVVFMNVSLNIMQQLINTVYTFIIPPLIIINYGSDINGL
                     ISSVKQCLVYLALVEAGVGGAAIVALFKPLSEGDRVLQSQIISEAKYFYSKIGVVFII
                     LLLLFSIIYAQIISDQINIILAISLFVIMGLINAVDFLLISKYQVFLTADNKLYVTSA
                     TKILTIILDMGCAYILITEGMNIILVQICSVFIYMIRYVIYYFYFLKKYSYISFDLKN
                     KTALLHQKSAVLIHQISGALVFNSPMILITIFLNLKAASIYSIYMLIFMAIKVFLSTI
                     TNGMQSVFGRMIVNENINAVREYFSKFEILYFSCLGWFYSCALILSYDFIYLYTLKFN
                     DPDYVQTILIYLFVIIGIADNIRVPGMTLIAAKGHYEQTKYRSIIEALLNIVFSIVFI
                     KIYGLPGVLLGSIISYSYRTIDIIYYSSNKILKQSIIFKVIMLFFLVFFILANYFIVE
                     SLKMPFFINDFFKWILYSTFVAVLSALLWLLFYKIMRLMNDKTY"
BASE COUNT     3995 a   1621 c   2129 g   4037 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcataattg aaatgtacat caaaatttat
       61 ggtttttctc taatggaaaa tataaccatt tacgattatg taattgtaaa taacggtcta
      121 tttaataaag atgctgtaat tgtttttttt ataatgttct atttgtttgg ttgtcttatt
      181 aatattgtat ccaattatgt tagctgtatt tatgttctat tggtactaat accattatca
      241 ctattattcg cattaaatgg acctgataaa gcatcgtatt tgtctttata taatgcaata
      301 gatacagtga gcgatgtttt taaatattac aatacaatag attctccatt atattatata
      361 acaatggtct tatttaaaaa tatagggctt acattttaca gttttatggt agtgcaaaat
      421 ttaattatat cattttcatt atcatatcta atttataaat ataaatgggg gtatcacata
      481 gcaatgtggc tatattttac ccatctactc gtaccatttt cggttcgtgg acatttggtc
      541 ataattacca ttttatttgc aaataggagt gtggcatcta aaattataag ttcgttagga
      601 ttagtggcga ttcatccact aatatctgca ttaccaatca cttatcaatt gcgtggcttg
      661 ataagaggta aacgtagtat tctaataatc gtgctaacaa tatgtgcgct agtattttca
      721 gcatcctatt tgattagtaa gtatactagt tatgctataa acggtgatga gtatgttaaa
      781 aacacggggt tatcactaat atatatagtt gatgcaatag agttattgct attttggtca
      841 ataattagac caatagcttt aatcgataag ttatttttac ttttcccggt cgtattaagt
      901 tttggtacaa taataatccc aatgcttggt cgattttcat atgcatgtga tttggtatat
      961 attttaattt taattaataa cgcaaatagg ctaaaaaata aaaagacact ttttatgtgt
     1021 ttatcactta ttgcattaat ccgatttgtt aaaaattatt attcaattga gtattttata
     1081 ttatgatgct tttttttaaa aaccccaaaa ataaaaaatc aactggtatt ctatatctcc
     1141 agacgttatt acatgaaagg ttatcaaatg taagagtgat aacatcaacg ataatgatta
     1201 ttaaacgtta tttcaatata atgaaatcta cagaggtagc aatttttctt ccctttactt
     1261 tctcttgcat atttgcaaaa aaaaatgatt tggtaataat gcctgatgac tcaattcgat
     1321 caataaccag cgtttcaaaa gtgaggtatg aaaataaaaa aattttaaaa tggctatttc
     1381 agaatatcag gttaattata actaagaata taatatcttt aaaaaaagac gttgtctatt
     1441 taaccgtctc taaagatgat cagtggtcat tgcaatcaaa attcccaaag cataaaattg
     1501 tctatattcc tcatcctata caatctgcaa gtaataaaga agatgtgttg gatagatgcg
     1561 aaaatataag tactatatgt tttatgaatt tacaggaaca ttattctgtt aatccaagtg
     1621 cttttattcg gcctaaaaca aaagttaatc tgaatgattt gcataataaa gttaatatta
     1681 tttttcatgg ttcttattct caagcctggt acgggaagta tatgtattat tatgggaaag
     1741 caaattgcaa agcagtaagg tatgtcgagg atttcgaaca gttctataat aatatagatc
     1801 taatcatttt gccattagca gccggtgcgg gaattaagaa tatcgttcta aattcagtat
     1861 acaggaataa acttatttta ggtaccgaag aggcattttc aggaatacct aaggaattta
     1921 caagaaaatt aattattgat tcgaatgatg acatcttaac aaaaattaac tcagaagtag
     1981 agtgtttagc cattaaggaa agttgtaata ttttacgtga ctatctagtc gttgaacaca
     2041 cagaaaaagc ttttatggat acaattttta aaaacataat ttaaagagca aatatgtcta
     2101 ttataagatt acaaggcgga cttggaaatc aactttttca gttctcattt gggtatgcgc
     2161 tttccaaaat taatgggaca ccattatatt ttgatataag tcattatgct gaaaatgatg
     2221 atcatggtgg ttacaggcta aacaatctac aaattccaga ggaatattta cagtattaca
     2281 caccaaaaat taataatatt tataaatttt tggttcgtgg gtcaagatta tatcctgaaa
     2341 tctttctttt tttaggtttt tgcaatgaat ttcatgccta tggttatgat tttgaatata
     2401 tagcgcaaaa atggaaatcc aaaaaatata tagggtattg gcaatctgag cactttttcc
     2461 ataaacatat attagatcta aaagaatttt ttattccaaa gaatgtgtct gaacaagcaa
     2521 atttacttgc agcaaaaatt cttgaatctc aatcatcact ttctattcat ataagaagag
     2581 gagattatat aaaaaacaaa acagctactt taactcatgg cgtttgttcg ttagagtatt
     2641 acaaaaaagc tttaaataaa atacgcgatt tggcaatgat acgtgacgtg tttattttca
     2701 gtgatgatat tttttggtgt aaagaaaata tcgaaacatt actcagtaaa aaatataata
     2761 tatattattc agaagattta tcacaagaag aagatttatg gttaatgagc ttagctaacc
     2821 atcatattat agcgaatagt agttttagtt ggtggggggc ttatttaggt acatcagcgt
     2881 cacagattgt tatttatcct actccttggt acgatataac tccaaaaaat acttatatcc
     2941 ccatagtcaa tcactggata aacgtggata aacatagctc gtgttaggga tagattataa
     3001 aggagcttgt agtgaagaga ccatactttt cgtttttatt atgcgttaac aaagatgaag
     3061 gttttctcaa tgatgctctt gagagtatta taaaccaaga ctattcttat gaatatgaaa
     3121 ttattgttat tgcaaataat tgtgatgata atctatgggc atatttgaac tccgttgaga
     3181 gtgacaaatt gaaaatacat cgtaccaata ttgggcagtt agcttttaat ttaaactatg
     3241 gagcaaatat tgctagaggg gagtatattg ttaggatgga tgccgatgat gtatccaccc
     3301 cgaatcgatt aaagattctt gaggcggcta ttcaagaaaa taactatcca gatgtaatag
     3361 gaacttcagt ttattttata agtgagacag gtagaatcat taagaaatat gttcctcctt
     3421 taggagaggt aaaaagtgct ctcattaaaa atccattcgt tcacccaact gtggccatca
     3481 aaaaagtgtc attatttaaa attgggggct atcttggtgg atttcaatca gaagataatg
     3541 atttatggat tcggatgctt agagcgcatg aatttaagtt agtaaatatt gattctgtaa
     3601 cattatttta tagaataaac gatcatcaaa ctcgtggtcg agttctacca tatgcagaag
     3661 ttgcatctta tgcgttgaga gaatttcttt taaaaaagag aataaaatat tttgcggcgc
     3721 ttcttatcaa tattggaaag gcgcttatac ttccaaaaag ataaggtatg aaacttatga
     3781 aaataatgca tacggtatta aatacattta ctgcaaagaa gtttgttgag ccgcttttag
     3841 ataatgttaa tgagcaacac aataaagaaa aaataattca taaaattttg attgatcctg
     3901 ttaatggtga tgagcgtttt ttaaaagaac ttcatgtaca ttatgaaata aaaaaatttg
     3961 atatagatta tttatttatc aaacatataa ttgagcttta tttctatttt agaaaagaaa
     4021 gaccagatat agttatagcc catatgtcaa gaggggctat tctaccgtta atttctgctt
     4081 ttatagcccg agtacccaaa cgtatatatt ttaatcatgg ggtaccatat attggttata
     4141 agaaccttcc taggatgtgt tttaaattac ttgaaaagct aaattgtttt ttttctaacc
     4201 aagtattaac tgtttctcaa ggtatgattg agcctttaac cgctataact aaaaaaccag
     4261 tatcaatact tgggcctggc tctgcttgtg gtattgacca caatgagttt caaagaaagt
     4321 caaaatatga tatagaaaat ataaaacagc aatacgctgc atcagatact ttcatcttta
     4381 ccttcgtagg aaggccggtt gcacgaaaag ggtttgatat aacgctgaaa gcgtttacta
     4441 aggcatttag tggtgcagat aatgtaattc ttttattagc aggggcaact tcggaagata
     4501 tttttcgcgt attaggaagt aaacctaata atatcaaacc attaggtttt gtaatggatt
     4561 tagataacgt atatttagct acagatgtag ttgtcgtacc aagtgagcat gagggttttt
     4621 gctacgcact tttagaagga gctgcaagag agtgcgctct catttgtagc gatatccctg
     4681 ggcctgacgc aattgttatt aataaaagaa atgggatttt aataacccct aaaaatattg
     4741 atagccttat ggttagtatg tctgaattat ttaatgatag agaaatgaca aaaaaatatg
     4801 gagaacaagc atatatgata gcttccgaat ttgaacgaaa aaaaatcatg ttatattata
     4861 gttactacat aagaagttta tttattgaac aaaggtgatt tttaaatgta attatccgta
     4921 tacaactaat actgatataa tttttgttta atctcattag caggtatttt atgaaaaaga
     4981 aagctctgat tactggaatt actggacaag atggttctta tcttgccgaa tttttattag
     5041 ataaaggtta tgaagttcat ggcataaaaa gaagagcctc tctttttaac actaatagaa
     5101 ttgatcatat ttatcaggat ctacatcatg agaaacaaag tttttttctt cattacggtg
     5161 atcttaccga ttcgttgaat atagttagat tggtacaaga aatacaacct gatgaaattt
     5221 ataacttagg ggcacaatcg cacgtagcag tatcatttga atcaccagaa tatactgctg
     5281 atgttgacgc gattggtacg ttacgattgt tagaagctat tcgtatatgt gggcttgaga
     5341 aaaaaactcg tttttatcaa gcctctacct cggaattgta tgggttagtg caagaaatac
     5401 ctcaaaagga gactacacca ttttatccaa ggtcacctta tgcagtagct aagatgtatg
     5461 catattggat tacagtcaat tatagagaat cttatgggat atatgcttgt aatggaattt
     5521 tatttaatca tgaatcacct cgccgtggtg aaacatttgt aacaagaaaa atcacccgag
     5581 cattagcaaa tattgcgtac ggattagagc catgtttgta tttaggcaat atggatgctt
     5641 tgcgcgattg ggggcatgca aaagattatg taagaatgca atggttaatg ttacaacaag
     5701 aaacacctga tgatttcgta atagctacgg gcaagcagat ctctgtaaga gagtttgtga
     5761 gaatggctgc tgctaattta ggtattgagc ttgaatttat tggttgtggt ttgaaagaag
     5821 taggagttgt gaaagcaatt catggaaatg aagctccttc ggttaaaatt ggcgatgtta
     5881 ttgttcaagt ggatcctcgt tattttcgac ctgccgaagt tgaaactttg ctcggttatc
     5941 ctgaaaaagc caaaataaaa ctagggtggg aacctgaaat aacgatcgaa gaaatgtgta
     6001 aggaaatgat aagaaatgat cttaacatag caaagcaaca ttctctgtta aagttaaatg
     6061 ggtatgatgt gtccttgtca gtggagaaat aatgaaatga aaatgaaaaa aatatatatt
     6121 gcagggcata gagggatggt tggttctgca atcaaacgtc agttaatgaa gaatagttct
     6181 gttgagttaa tttttcgtag tagtcacgaa cttgacttga tgaaccaaga tgctgtccaa
     6241 aattttttta atacagaaag gatcgatgaa gtctatttag cagctgctaa agttggtggg
     6301 atatatgcga ataataaata tccagctgac tttatatata ataatatact catacaagcc
     6361 aatgtaatca attcagctca tagagctggg gttcaaaaat tattattttt aggctcatca
     6421 tgtatctatc ctaaatttgc agaacaacct ataaaagaag aatccctact ttgtggttca
     6481 ttagaaccta caaatgaacc ttatgcgatt gcgaaaataa cagggattaa gttgtgtgag
     6541 tcttataata gacaatatgg tcgtgattat aggagcgtta tgcctacaaa tctttatggg
     6601 cctaatgaca attttcatca tgaaaattca catgtattac ctgccttaat acggcgtttc
     6661 cacgaagcaa agattaataa tcttccagag gtcagtgttt ggggttctgg caatgcgtta
     6721 cgagaatttc ttcatgtaga tgatatggca gctgcatcca tatatataat gaatttacct
     6781 aatgaacgtt ataaagaata tactaaacca gagtgctcac acataaatgt tggttctggt
     6841 aaagattatt caattcggca attggcagaa atggttgcgg aaattactaa ttataaagga
     6901 aatatttact ttgatagtac taaacctgat ggaacacttc gtaaattaat gagcaatgaa
     6961 cgattgagta aactaggctg gtcacccaaa atcgagttat atgatggggt aaaagatact
     7021 taccagtggt ttttgaataa tatagaaaat ataagaaaat aaaaatctta cttaaaaatt
     7081 atgggtaggt cctactggaa gaaatatttt ataaatgaca tttatatttg tacagaggtt
     7141 atagtagatg ctttttccga taattatggc tggtggcacc ggtagtcgac tttggccact
     7201 gtctcgagag tcttttccaa agcaatatct aaaactttat ggtgacatga caatgttaca
     7261 atctacaatt gcgagattaa atacattgga ttgttcgacc ccattagtta tttgtaatga
     7321 agagcataga ttcatcgttg cggaacagtt aaagaaaatt aataaattag ataagaatat
     7381 tctgcttgaa ccacaaggta aaaatacggc cccagcaata accttgtgtg cgtttgctgc
     7441 tttaaaatat gcaaaaaatg atgaggatcc attactactt gtattggctg ctgatcatgt
     7501 aataggaaac atatcttctt ttactaagtc agttaaaaat gcattaccgc atgctcatgc
     7561 tggcaagctt gtaacatttg gtataatacc tacaacacct gaaactggtt atggatatat
     7621 tcaccgagga gaaaaattta atgatgcgta caaagtttca tcttttacag aaaagcctga
     7681 tatttatcgt gcgcaagaat ttttaaaaac aggtgaatat tattggaaca gtggtatgtt
     7741 cctatttagt gctaaacgac tgcttgcaga gatggaaaag tatcgcccag atattttttc
     7801 agcatgtgaa caagcttttt tatcgccatc taatgatcta gattttattc gtgtggatcg
     7861 tgataagttt tcgttatgtc ctagtgagtc aattgactat gctgtgatgg agaatacgga
     7921 tgatgccatc gttgtaccta tggatgcaga atggtctgat gttggttcgt ggtcttcatt
     7981 atgggatatt tcagataagg atgtaaatgg gaatgctgtt agtggtgatt tgattagttt
     8041 taactcttat aataatttta ttaaagctga agataaacta gttgctttgg ttggcgttaa
     8101 tgatttagtc ttggtacata cggatgatgc tatattagtg tcaagcaaat atgaagtgca
     8161 aaatgtaaaa aaaatagttg aagtccttaa ccaaaacggc agagatgagt cgaaaaaaca
     8221 taaagaagta tatcggcctt ggggtgaata tcattatatt acggaggcag agaaatacaa
     8281 cgttagacgc gtactaatta atccaggtca aaaaatctca acgcaaacac acaaccatag
     8341 atcagagcac tgggttatag tttcaggaat agcgaaaatt acaattaata agaatataaa
     8401 tataattaaa gaaaatgaat ctatttatat acctcccggt actatgcata ccatagaaaa
     8461 ctcagaaatg ttgccattag aaataattga aattcaaacc ggtcaattat taactgaaga
     8521 tgacgttatt cgtccttaaa ctttaatgta aaagagaaat gaagatttgt aattataagt
     8581 agttattaag ctaattttaa tgaggtttta tgagcaagaa atttaatact ttatctatca
     8641 tcaggaatag tggcgttgcc tttggtacca gtggtgctcg aggcctcgtt acagaattca
     8701 cccccgaagt atgcggagct ttttctcatg cttttattaa cgtaatgaag caaaagtaca
     8761 agtttcatgg agtcgcatta gcaattgata atcggcctag tagttactct atagctcaag
     8821 catgtgcatc tgtgattgcg caatacggct taaaggtaaa ttattacggt gttatcccaa
     8881 caccggcatt agcttataag gcactggagg ataaaatacc gtgtataatg gtaacgggta
     8941 gtcacatacc acatgatcgt aatggattaa agttttatag cccaattggc gagatcacta
     9001 aggaagatga aattcgcatt aaagatgttt ctatagattt tacattggac tcatccatac
     9061 caaatttata tccgtgtcct gacgcagcta gttgttattt taaaagatac attgactttt
     9121 ttcctgaagg aattttaaaa ggtaaaagag ttggctttta cgagcattca agcgctggtc
     9181 gtgacttata cattcctatt tttgaaaagt taggagcaga gatagtttcc atcggtagaa
     9241 gtgataaatt tatcccaata gatacagaag ctgttggtca ggatcttgtt atccaggcaa
     9301 aaaaatgggc gttagaatat aaattagatg ctatttttag tactgatggt gatggcgatc
     9361 gcccaatgtt aagtgatgag aatggaaaat ggttgcgagg agatatactt ggactgttat
     9421 gtgctgaaac attgaatata aaatctattg cgattccaat tagttgtaac tcattaataa
     9481 tgcaacgtaa gtgttttgaa gaagttgttt taacaaaaat tggttcgcct tacgtattag
     9541 atgctttttc ttctttaaaa gataaatatc aaaattatgc aggttttgag gctaatggtg
     9601 gttttatatt aggttcggat ataacagttg ataatagaaa gatcaaagcc ttacctacac
     9661 gagatgcatt actccctata ttagttttac tttctgcaaa taaaaaaatt tcaagcttgg
     9721 tacatagctt accaagtaga tttacttatt ctgaccgaat tgctaatttt cttccagaaa
     9781 aaagagatag gttatttaca atatgtaaaa ataatccaca tacaatagtt gatcttttgg
     9841 gagagaatga ggcgggcatt gaagttaaag atataaataa tttagacggt tatagattta
     9901 cactaaataa taatgtcatc attcatctaa gatcttccgg aaatgctcca gagttgagat
     9961 gttattgtga ggctgacagt gagttttgtg ctcaaaaata tgtggagaga attttaagtt
    10021 cggttgcttg ccttataaaa ataaattaat aatttaaggt tgtttagttt ttagtgcagt
    10081 ttcacctttt catgactgca ctatcatttt atatcaaaac ttaacaagct agacagcaag
    10141 gttgacgagg caatgcagaa taataaacgc gttgtattta tgaatgtgtc tctaaatata
    10201 atgcagcaac tgattaatac ggtatatacc tttatcatac ccccattaat tattataaat
    10261 tatggttcgg atataaatgg tttgatttct tctgttaaac aatgccttgt atatttagct
    10321 ctcgtagagg ctggtgttgg aggggcagct attgtagcac tattcaaacc attgtcagaa
    10381 ggggatcgtg tgctacaaag tcagatcata tcggaggcta aatattttta ctcaaaaatt
    10441 ggtgttgtat ttattatttt acttttatta ttttcaataa tatacgcaca aataatttct
    10501 gaccaaatta acatcattct agcaattagt ctttttgtta ttatggggct aataaatgcg
    10561 gtggattttt tattaatatc aaagtatcag gtttttctta ctgctgataa taaattatat
    10621 gtaacttcgg ctacaaaaat attaacaatc atattagata tgggatgtgc ttatattctg
    10681 ataactgaag gtatgaatat tattttggtg caaatttgct ccgtgtttat ctatatgatt
    10741 agatatgtta tttattattt ttattttctg aaaaaataca gttatatatc atttgatcta
    10801 aaaaataaaa ctgcattgct tcatcagaaa tcagcagttt taattcatca gatatcagga
    10861 gcattagttt tcaatagccc aatgatattg attactattt ttcttaacct aaaggcagca
    10921 agtatatatt ctatttatat gcttatattt atggccatta aggttttttt atcgaccatt
    10981 accaatggta tgcaatccgt attcggccgt atgattgtta atgaaaacat taatgctgta
    11041 agagaatatt tttcaaagtt tgaaatttta tatttttcat gtttgggatg gttctattca
    11101 tgtgctttga ttttatcgta tgattttata tatttatata ctttaaaatt taacgatcct
    11161 gattatgttc aaactatatt gatttatctt tttgttatta taggtatagc tgataacatt
    11221 cgcgtcccag gaatgacatt aatagcggct aaaggtcact atgaacaaac aaaatacaga
    11281 tctattattg aggctttatt aaatatcgtt ttttcaatag tttttattaa aatatatggg
    11341 cttcctggtg tattgctagg ttccattatc tcttattcat acaggacaat agatattatt
    11401 tattatagta gcaataaaat tttaaaacaa tcgataatat ttaaagtgat tatgttattt
    11461 tttttggttt tttttatttt ggctaattat tttatcgttg aaagtttgaa aatgccattt
    11521 tttataaatg attttttcaa atggatttta tattcaactt ttgttgcagt tctatcagct
    11581 ttgttatggt tattgtttta taaaattatg aggctaatga atgataaaac ttattaaatt
    11641 ttttatgcgt aaattgtaac ttttatattg catactggta tattttaaag attttcatac
    11701 aagccgctac ttatatgcgg taaccccctg acaggagtaa acaatgtcaa agcaacagat
    11761 cggcgtcgtc ggtatggcag tg
//
