LOCUS       AB812057               16946 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: N282.
ACCESSION   AB812057
VERSION     AB812057.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16946)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="N282"
                     /db_xref="taxon:562"
                     /note="serogroup:O133"
     gene            <1..896
                     /gene="wcaM"
     CDS             <1..896
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01666.1"
                     /translation="LRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHV
                     IERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKH
                     FVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKY
                     LSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHL
                     FMRNINVIQGSSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNERGQSSVDID
                     RINHHIVNVEKINFRLPERRE"
     gene            1071..1964
                     /gene="galF"
     CDS             1071..1964
                     /gene="galF"
                     /note="O133_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01667.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            2335..3420
                     /gene="rmlB"
     CDS             2335..3420
                     /gene="rmlB"
                     /note="O133_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01668.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            3420..4319
                     /gene="rmlD"
     CDS             3420..4319
                     /gene="rmlD"
                     /note="O133_03"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ01669.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWIIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            4377..5255
                     /gene="rmlA"
     CDS             4377..5255
                     /gene="rmlA"
                     /note="O133_04"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01670.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNNVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVRVLAEPL
                     KKNAYGQYLLKMVKGY"
     gene            5260..5796
                     /gene="rmlC"
     CDS             5260..5796
                     /gene="rmlC"
                     /note="O133_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ01671.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIIWNDPELQINWPVD
                     GELSLSKKDMKQPKFNVI"
     gene            5836..7077
                     /gene="wzx"
     CDS             5836..7077
                     /gene="wzx"
                     /note="O133_06"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01672.1"
                     /translation="MNKILKDIPIKSILHLFIVRGSIYLFPFITLPIITRNLGVAHFG
                     ILSLFLAAQQYFVMLVEYGFTLTGARDIVRCDNKQDEMKIINEIILCRLLIFLFCTAI
                     IIGAYAFFTEHGYISCYLILWSTVFCSVFNQTHFFIGKEKTGFIVISTCITRIVSIVI
                     VVLYVKDNRDINIALLAYSINTALPNILSTFYLNLYLKYKVKIIFCNNEIIDRFKSGY
                     DIFISNVFTSIYSTLTLMYLGTAKGSIETGYYSSADKLKAAAQGILFPVAQAFFPRVS
                     KECGISFYLLWRKSTTILVCFAVAIVASLFIFSNLIYILLLGEQYLSGINIYYILTLS
                     IIPISFGISYAQNLYLTQGQTKLLRKIYFAVSVLHLFHMPILVHFLGAIGAAISVLIT
                     ESFASLLMFIFRKKCYTWSQA"
     CDS             7062..7955
                     /note="O133_07"
                     /gene="wbwV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01673.1"
                     /translation="MESSLKIFAVIVTYNPDMDSFIRNLSAILGQVDTIIIVDNSNEE
                     NYQISLKNHVDNISNVILIQPNENLGIAYAQNIGFMQSLKLNADFVITFDQDSSVDSD
                     LIEVLYSEYTKIKDNYNYKIACIGPSVINERNNARYEKYFKNSTQLNESVYSVKSIIS
                     SGSLYSMDVFSAIGFNKAEWFIDSIDIEWCYRARYLGYHVLMTTNVAMKHNLGARDVK
                     LPGGKSINIGSPFRLYYVFRNWIFSLREPQFDIKYKMKILLMMPIKFIIFSTVSPRVS
                     RFRFMLKGIKDGLLKKHSFIK"
     CDS             7961..9067
                     /note="O133_08"
                     /gene="wcmT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01674.1"
                     /translation="MKISITANTSWYLYNFRRNTISALLNAGYEVIALSPDTEYKSKL
                     ETLGCKCFTIKMQRWGKNPLQDLQTILSFYTIFKKEKVNITLNFTPKNNIYATLAANL
                     LNIKVINNIAGLGVAFIQNTLFSKLVSYLYKVSQRKADYIFFQNEEDKELFLKLKIVS
                     ENKCERIPGSGVDLKRFEYSPIENTKNIRFLLVARLIAEKGIYIYVEAARAIKKLFPD
                     TEFYLLGPLDTCSTSGIKKYELDQWQKEKIITYLGFTDSVELELIKAHCVVLPSYYRE
                     GVPKSLLEAAAIGRPIITTDNIGCREVVDHGINGFLCEPGSIISLADNMERFLTLSSM
                     EKEIMGRNSRAKIQKDFDEKIVIEKYFSVVNSLK"
     gene            9381..10538
                     /gene="wzy"
     CDS             9381..10538
                     /gene="wzy"
                     /note="O133_09"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01675.1"
                     /translation="MAYLFISLLFVLLFAEIKITQRKFSPTIVLITLWIGVLIVTQIY
                     SSALWQLSVETMWWVFGGVFAFFIGGFWGKLFFKIYGFKNISLQSNGNTLLFAIPFYI
                     LMFFFYYFMVRAGANEDQNWYVGIRKIINYGEPDLFFIMFGYIYYLIYPMLYLEAARY
                     YSSKSFNKKDKYNYHMQLIMCILYAVMSTAKLKLLLVIVPVFFIRSYFKPTSTKLIIV
                     VGTMFLGVFFLSLILLNKIAGDIGVFKIIITSIGNYTFFNLYAFDSLHFKEFLKIKCI
                     DNSNSCSLLPFFEYGDFRTNIYTIMYSFIEYGFFSYSLFLFMVGFIHNAADQIAKKTS
                     NIYAIILSSVLYVPLVFQIMDNQYTASKYLIYILMFSYVVYFTNVRRLRVM"
     gene            10714..12120
                     /gene="gnd"
     CDS             10714..12120
                     /gene="gnd"
                     /note="O133_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01676.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENSGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            12369..13535
                     /gene="ugd"
     CDS             12369..13535
                     /gene="ugd"
                     /note="O133_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01677.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(13601..14605)
                     /note="O133_12"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01678.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            14957..15991
                     /gene="wzz"
     CDS             14957..15991
                     /gene="wzz"
                     /note="O133_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01679.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNLEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQETMFLLGSDALESMIKREATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(16088..16699)
                     /gene="hisI"
     CDS             complement(16088..16699)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01680.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPGTSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4957 a   3206 c   3723 g   5060 t
ORIGIN
        1 tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaac tgtaaattta
       61 gtgatttaca gggcgacgcc attgaatgga acgtggcgat taacgaccgt gatattttga
      121 tatctgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac tggggcatcg
      181 gcataggtct tgcgggaagc acttatgata ataattaccc ggaagaccag gcagtgaaaa
      241 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
      301 gtaaacattt tgttatccgt aatatcaaag cccgcaatat cacgccggat ttcagtaaga
      361 aagcaggtat tgataacgcg acagttgcta tttacggttg tgacaatttt gtgattgata
      421 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
      481 atctctcgat accgcaaaat ttccgagtga ataatattca actggataac acccatcttg
      541 cttataaatt gcgcggcatc caaatctccg ccgggaatgc tctctccttt gtggcgctga
      601 ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa catcttttta
      661 tgcgtaatat caatgtgata cagggatcct ctgttggacc cgcattgatc atgaacttcg
      721 acatgcgcaa agacgttcga ggcgtcttta tggcgaaaga agaaacactg ctgtctcttg
      781 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
      841 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
      901 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
      961 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
     1021 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
     1081 taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc actaaggcga
     1141 ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga
     1201 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
     1261 aaaaccactt cgacacctct tatgaattag aatctcttct tgaactgcgc gtgaagcgtc
     1321 aactactggc ggaagtgcag tccatctgtc cgccgggcgt gacaattatg aacgtgcgtc
     1381 agggcgaacc tttaggtttg ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
     1441 acccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc gacccgctgc
     1501 gctacaacct tgctgccatg attgcgcgtt tcaatgaaac gggccgtagc caggtgctgg
     1561 caaaacgtat gccgggtgac ctctctgaat actccgttat tcagaccaaa gaaccactgg
     1621 atcgcgaagg taaagtcagc cgcatcgtcg aatttatcga aaaaccggat cagccgcaga
     1681 cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgcagat atttggccgg
     1741 aacttgaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat gccatcgctg
     1801 aactggcgaa aaaacagtcc gttgatgcca tgctgatgac aggtgacagc tacgactgcg
     1861 gtaaaaaaat gggttatatg caggcgtttg tgaagtatgg actacgcaac ctgaaagaag
     1921 gagcgaagtt ccgcaaaggc attgagaagc tgttaagcga ataatgaaaa tctgaccgaa
     1981 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa gtaattgttg
     2041 cgaattttcc tgccgttgtt ttatataaac aatcagaata acaacgagtt agcaatagga
     2101 ttttagtcaa agttttccag gattttcctt gtttccagag cggattggta agacaattag
     2161 cgtttgaatt tttcgggttt agcgcgagtg ggtaacgctc gctacatcgt aggcatgcat
     2221 gcagtgctct ggtagctgta aagccagggg cggtagcgtg cattaatacc tctattaatc
     2281 aaactgagag ccgcttattt cacagcatgc tctgaagtaa tatggaataa taaagtgaag
     2341 atacttgtta ctggtggcgc aggatttatt ggttctgctg tagttcgtca cattataaat
     2401 aatactcagg atagtgttgt taatgtcgat aaattaacgt acgccggaaa cctggaatca
     2461 cttgctgatg tttctgattc tgaacgctat gtttttgaac atgcggatat ttgcgatgca
     2521 gctgcaatgg cacggatttt tgctctgcat cagccggatg cagtgatgca cctggctgct
     2581 gaaagccatg tggatcgttc aattacaggc cctgcggcat ttattgaaac caatattgtt
     2641 ggtacttatg tccttttgga agccgctcgc aattactggt ctgctcttga tggcgacaag
     2701 aaaaatagct tccgttttca tcatatttct actgacgaag tttatggcga tctgcctcat
     2761 ccagatgaag taaataatac agaagaatta cccttattta ctgagacgac agcttacgcg
     2821 ccaagcagcc cttattccgc atccaaagca tccagcgatc atttagtccg tgcgtggaaa
     2881 cgtacctatg gtttaccgac cattgtgact aattgttcga ataactacgg tccttatcac
     2941 tttccggaaa aattgattcc actagtaatt cttaatgctc tggaaggtaa ggcattacct
     3001 atttatggca aaggggatca aattcgcgac tggctgtatg ttgaagatca tgcacgtgcg
     3061 ttatataccg tcgtaaccga aggtaaagcg ggtgaaactt ataacattgg tggacacaac
     3121 gaaaagaaaa acatcgatgt agtgctcact atttgtgatt tgttggatga gattgtaccg
     3181 aaagagaaat cttaccgcga gcaaattact tatgttgccg atcgtccggg acacgatcgc
     3241 cgttatgcca ttgatgctga gaagattggt cgcgaattgg gatggaaacc acaggaaacg
     3301 tttgagagcg ggattcggaa gacagtggaa tggtacctgt ccaatacaaa atgggttgat
     3361 aatgtgaaaa gtggtgccta tcaatcgtgg attgaacaga actatgaggg ccgccagtaa
     3421 tgaatatcct ccttttcggc aaaacagggc aggtaggttg ggaactacag cgtgctctgg
     3481 cacccctggg taatttgatt gctcttgatg ttcactccac tgattactgt ggtgatttta
     3541 gtaatcctga aggtgtagct gaaaccgtaa gaagcattcg gcctgatatt attgtcaacg
     3601 cagccgctca caccgcagta gacaaagcag aatcagaacc ggagtttgca caattactta
     3661 acgcgacgag tgtcgaagcg atcgcgaaag cagccaatga agtcggcgcc tggattattc
     3721 actactctac tgactacgta tttccgggga ccggtgaaat accatggcag gaggcggatg
     3781 caaccgcacc gctaaatgtt tacggtgaaa ccaagttagc tggagaaaaa gcattacaag
     3841 agcattgtgc caagcaccta attttccgta ccagctgggt ctatgcaggt aaaggaaata
     3901 acttcgccaa aacgatgttg cgtctggcaa aagagcgtga agaattagcc gttattaatg
     3961 atcagtttgg tgcgccaaca ggtgctgaac tgctggctga ttgtacggca catgcaattc
     4021 gtgtggcact gaataaacca gaagtcgcag gcttgtacca tctggtagcc agtggtacca
     4081 caacctggca cgattatgct gcgctggttt ttgaagaggc acgaaaagca ggtattcccc
     4141 ttgcactcaa caagctcaac gcagtaccaa caacagccta tcctacacca gctcgtcgcc
     4201 cacataactc tcgccttaat acagaaaaat ttcagcagaa ctttgcgctt gtcttgcctg
     4261 actggcaggt tggtgtgaaa cgaatgctca acgaattatt tacgactaca gcaatttaat
     4321 agtttttgca tcttgttcgt gatggtggag caagatgaat taaaaggaat gatgaaatga
     4381 aaacgcgtaa aggtattatt ttagcgggtg gttctggtac tcgtctttat cctgtaacta
     4441 tggctgtcag taaacagctg ttaccgattt atgataaacc gatgatctat tatccgctct
     4501 ctactctgat gttagcgggt attcgcgata ttctgattat cagtacgcca caggatactc
     4561 ctcgttttca acaactgctg ggtgacggga gccagtgggg gttaaatctt cagtacaaag
     4621 tgcaaccgag tccagatggt cttgctcagg catttatcat cggtgaagag tttatcggtg
     4681 gtgatgattg tgctttggtt ctaggtgata atatctttta cggtcacgat ctgccgaagt
     4741 taatggacgt agctgttaac aaagaaagtg gtgcaacggt atttgcctat cacgttaatg
     4801 atcctgaacg ctacggtgtc gttgagtttg ataaaaacgg tacggcaata agcctggaag
     4861 aaaaaccgct acaaccaaaa agtaattatg cggtaaccgg gctttatttc tatgataaca
     4921 acgttgtcga aatggcgaaa aaccttaagc cttctgcccg tggtgaactg gaaattaccg
     4981 atattaaccg tatttatatg gaacaggggc gtttatccgt tgccatgatg gggcgtggtt
     5041 atgcatggct ggatacgggg acacatcaga gtcttattga agcaagcaac ttcatagcaa
     5101 caattgaaga gcgtcagggc ttgaaagttt cctgtccgga agaaattgct taccgtaaag
     5161 gatttattga tgctgagcag gttagggtat tagccgagcc acttaagaaa aatgcttatg
     5221 gtcaatattt gctgaaaatg gttaaaggtt attaataaaa tgaatgtaat taaaacagaa
     5281 attccagatg tactgatttt tgaaccgaaa gtttttggtg atgagcgtgg tttctttttt
     5341 gagagcttta accaaaaggt ttttgaggaa gctgtaggcc gcaaagttga atttgtccag
     5401 gataaccatt cgaagtcttg taaaggtgtt ttacgcgggc tgcattatca gttagaacct
     5461 tatgcgcaag ggaaattggt acgttgcgtt gttggtgagg tttttgatgt tgcggttgat
     5521 attcgtaaat cgtcaccaac ttttggaaaa tgggttgggg tgaatttatc tgctgagaat
     5581 aagcgccagt tgtggatacc tgaaggattt gcgcatgggt ttttggtgct gagtgaaact
     5641 gctgagtttg tttataagac gacgaattat tatcatccag agagtgatag agggattatt
     5701 tggaatgatc ctgaactaca aattaattgg ccagtagatg gagagttaag tctttctaaa
     5761 aaagatatga aacaacctaa atttaatgtt atctaatata atgccccctt aagtgaatta
     5821 aggggggaat ataatatgaa taaaattcta aaagatatac caattaagtc tatattgcat
     5881 ctgtttattg ttcgagggag tatctattta ttccctttta ttacattgcc gatcataacc
     5941 cgcaatttag gggttgctca ctttggcata ttatccttat ttcttgctgc gcaacaatat
     6001 tttgttatgc ttgttgaata tggcttcaca ttaactggag caagagatat tgtcaggtgt
     6061 gataataaac aagatgaaat gaaaattatc aatgaaataa tactgtgcag gctactaata
     6121 tttctatttt gcacagcgat tatcattggt gcgtatgctt tttttacaga acatggttat
     6181 atctcctgtt atttaatttt atggagcacg gttttttgct ctgtatttaa ccaaacacat
     6241 ttttttattg gaaaagaaaa gacaggtttt attgtaatat ccacatgtat tactcgcata
     6301 gtttcgatag taattgtggt tctttatgtt aaagacaata gagatatcaa tattgctctt
     6361 ttggcatatt caattaatac agcattacca aacatcttgt caactttcta tcttaatttg
     6421 tatctcaagt ataaagtaaa aattattttt tgtaataacg aaataataga ccgttttaaa
     6481 agtgggtatg atatattcat ttcaaatgtt ttcacaagta tttatagtac attaacgtta
     6541 atgtatttag ggactgcaaa aggatctata gaaacaggct attattcaag tgctgacaaa
     6601 ttaaaggctg ctgctcaagg tattttattt cctgttgcgc aggcattttt ccctagagtt
     6661 tcgaaagaat gtggcatcag cttttatttg ttatggcgaa aatcgacgac tatattagtt
     6721 tgtttcgcag tagcaatcgt agcaagccta ttcatattca gtaatcttat ctatatattg
     6781 ttactcggag agcaatattt atcaggtatt aatatttatt atatattaac tttatctata
     6841 atccctattt catttgggat ctcttatgcc caaaacctat acttgacgca agggcagaca
     6901 aaactacttc ggaaaatata ttttgccgta tctgtactac atttgttcca tatgccaata
     6961 cttgtgcatt ttttgggcgc aataggtgcc gcaatctcgg ttttaatcac tgaatcattt
     7021 gcgtctctac taatgtttat ctttaggaaa aaatgttaca catggagtca agcttgaaaa
     7081 tatttgcagt aatagtaacg tataatccag atatggattc tttcattaga aacctatcag
     7141 caattttagg gcaagttgat acaattatta ttgttgataa cagtaatgaa gaaaactatc
     7201 agatttcttt aaaaaatcat gtagataata tcagcaatgt gattttaatt cagccaaatg
     7261 aaaatctcgg tattgcatat gcacaaaata taggatttat gcaatcatta aaattaaatg
     7321 ccgattttgt gatcacattt gaccaagact catctgttga tagtgatcta attgaggtac
     7381 tgtattcaga atatacaaaa ataaaagata attataatta taaaatcgct tgtatagggc
     7441 cctcagtaat taatgaaagg aacaacgcaa gatatgaaaa atacttcaaa aatagcacac
     7501 aattaaatga atcagtatat agtgtaaaat caattatatc gtcgggttct ttgtatagta
     7561 tggatgtttt ttctgcaatt ggctttaata aagcagagtg gtttattgat tctattgata
     7621 tagagtggtg ctatcgtgca agatatcttg gctatcatgt tttaatgaca accaatgtag
     7681 caatgaaaca taatttgggg gcaagagatg ttaaattacc aggagggaag tctattaata
     7741 taggttctcc ttttagactt tattatgtat ttaggaactg gattttttct cttcgagagc
     7801 ctcagtttga tattaaatat aaaatgaaaa tattattaat gatgccgatt aaatttatta
     7861 tattttctac cgtttcaccc cgtgtatctc gatttcgatt tatgttaaag gggatcaaag
     7921 atggactttt gaaaaaacat tcatttataa aatagaagac atgaaaattt caataacagc
     7981 aaacactagt tggtacttat ataactttag acggaatact atttctgcat tattaaatgc
     8041 aggatatgaa gtcatagctt tatctccaga tacagagtat aaatcgaagc tagagactct
     8101 cggttgtaaa tgtttcacaa taaagatgca aagatggggg aagaacccgt tacaagattt
     8161 gcaaacaatt cttagctttt atacaatctt taaaaaagaa aaagtaaata tcacacttaa
     8221 ttttacacct aaaaataata tttatgcaac attagcagct aatttgctaa atattaaagt
     8281 tattaataat attgccggat tgggggttgc gtttatacaa aatactttgt tttcaaaatt
     8341 ggtatcttat ctctataagg ttagtcaaag aaaggctgat tatattttct ttcaaaatga
     8401 agaagacaaa gagttgtttt taaaacttaa gattgtatca gaaaataaat gtgagaggat
     8461 tccaggttct ggtgtggatt taaagcgatt tgaatattcg cccatagaaa atacaaaaaa
     8521 cattcgattc ttattagttg ccagattgat agctgagaaa ggtatttata tctatgttga
     8581 agcagcaaga gctattaaaa aactattccc agatacagag ttttatttgc taggcccact
     8641 ggatacctgt agtacttcag gaataaaaaa atatgaactg gatcaatggc aaaaagaaaa
     8701 aataataact tatcttgggt ttactgattc agttgagctt gagctgataa aagcacattg
     8761 cgttgtatta ccatcttact acagagaagg agtaccaaaa tcattgttag aggccgcagc
     8821 aattggtaga cctatcatta caaccgataa tataggttgt agagaggttg tggatcatgg
     8881 tattaatgga ttcctttgcg agcctggctc aataattagt cttgcagata atatggaaag
     8941 atttttaact ttaagttcaa tggaaaaaga aataatgggt agaaacagta gagctaaaat
     9001 tcaaaaagac tttgatgaga aaattgtaat tgaaaaatat ttttctgttg taaattcgtt
     9061 aaaatgaaat gcttttataa atacatttat actctaaata attcgatttg caggaaaacg
     9121 atgaatccct aggagcttga attccgaaag ttactagagg gaaggaactc ggtcgtagca
     9181 catacaactt aaagtatgac gagtatataa gaaattgtag ctaatatcta tagtaaattt
     9241 gagaatatat tacaacttaa tcagtaaatt taattatttg tttaagattt tcatattctg
     9301 tacatgtggt tggatagaat atattatgat catgtgttct attttaaaat taaatcttac
     9361 ttattcctcg gatagtagaa atggcttatt tatttatttc gttattattt gttttgctat
     9421 tcgctgaaat aaaaataact caaaggaagt tttctccgac tattgtatta ataacattat
     9481 ggattggtgt tttaatcgta acccaaatat atagttctgc gttatggcaa ctgtcagttg
     9541 aaaccatgtg gtgggttttt ggtggggtgt ttgctttttt tataggtgga ttttggggga
     9601 agttattttt taaaatatat gggtttaaaa atatctctct acaaagtaat ggcaacacac
     9661 ttttatttgc tattcctttt tatattttaa tgttcttttt ttattatttt atggtcagag
     9721 caggagctaa tgaagatcaa aactggtatg ttggcatacg aaagataata aactatggtg
     9781 agcctgattt gttttttata atgtttgggt atatttatta cttgatctac ccaatgctat
     9841 atttagaagc tgcgaggtat tactcttcaa agagtttcaa caagaaggat aaatataatt
     9901 atcatatgca attaattatg tgcatattat atgctgtaat gtcaacggca aagcttaagc
     9961 ttttgctagt gattgttcct gtgttcttta tacggagtta ttttaaacca acaagtacga
    10021 aactaataat agttgttggc acgatgtttc ttggggtttt tttcttatca cttattttac
    10081 ttaataaaat agctggcgat ataggtgttt tcaaaataat tataacatca ataggtaact
    10141 atacattttt caatctatat gctttcgatt cacttcattt taaagagttt ttaaaaatta
    10201 agtgtattga taactcgaat agctgttcct tattaccgtt tttcgaatat ggtgatttta
    10261 gaacaaatat atatactata atgtattcat ttattgaata tggtttcttt agttatagcc
    10321 tgttcctttt tatggttgga tttattcata atgcggcaga tcaaatagca aaaaaaacat
    10381 caaatatata tgcaattata cttagctcag tgctttacgt tcctttggta ttccaaataa
    10441 tggataatca atatacggca tcaaagtatt tgatatatat attgatgttt tcttatgttg
    10501 tatatttcac taatgtgaga cgtttgcgag tgatgtaatt tatttgatta gttcaaaaag
    10561 cttgcgcggg ttataaatga aattattatt gaaagatatt ccattcattg aaatattttt
    10621 taacatttac gtatcgatag attttcttac tcaagaatac cacttttcaa gccgcggcaa
    10681 ctcgtggtga ccaccccctg acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc
    10741 ggtatggcag taatggggcg caaccttgcg ctcaacatcg aaagccgtgg ttataccgtc
    10801 tctattttca accgttctcg tgaaaagacg gaagaagtga ttgccgaaaa ttcaggcaag
    10861 aaactggttc cttactatac ggtgaaagag tttgttgaat ctctggaaac gcctcgtcgc
    10921 atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc tctcaagcca
    10981 tacctcgata aaggtgacat catcattgat ggcggtaaca ccttcttcca ggacaccatt
    11041 cgtcgtaacc gtgagctttc tgcagaaggc tttaatttca ttggtaccgg tgtttccgga
    11101 ggcgaagaag gcgcgttgaa aggtccttcc attatgcctg gtggccagaa agaagcctat
    11161 gaactggttg cgccgatcct gaccaaaatc gccgcagtgg ctgaagacgg tgagccatgc
    11221 gttacctata ttggtgccga tggcgcaggt cactatgtga agatggttca caacggtatt
    11281 gaatacggag atatgcaact gattgctgaa gcctattctc tgcttaaagg tggcctgaac
    11341 ctcaccaacg aagaactggc gcagaccttt accgagtgga ataacggtga actgagcagc
    11401 tacctgatcg acatcaccaa agatatcttc accaaaaaag atgaagacgg taactacctg
    11461 gttgatgtga tcctggatga agcggctaac aaaggtaccg gtaaatggac cagccagagc
    11521 gcactggatc tcggcgaacc gctgtcgctg attaccgagt ctgtgtttgc acgttatatc
    11581 tcttctctga aagatcagcg tgttgccgca tctaaagttc tctctggccc gcaagcacag
    11641 ccagcaggcg acaaggctga gttcatcgaa aaagttcgcc gtgcgctgta tctgggcaaa
    11701 atcgtttctt acgcccaggg cttctctcag ctgcgtgctg cgtctgaaga gtacaactgg
    11761 gatctgaact acggcgaaat cgcgaagatt ttccgtgctg gctgcatcat ccgtgcgcaa
    11821 ttcctgcaga aaatcaccga tgcatacgcc gaaaatccgc agatcgctaa cctgttgctg
    11881 gctccttact tcaagcaaat tgccgatgac taccagcagg cgctgcgcga tgtcgtcgct
    11941 tacgcagtac agaacggtat cccggttccg accttcgccg ctgctgttgc ctattatgat
    12001 agctaccgtg ccgctgttct gcctgcgaac ctgatccagg cacagcgtga ctatttcggt
    12061 gcgcatactt ataagcgcat tgataaagac ggtgtgttcc ataccgaatg gctggattaa
    12121 tctgatttaa atcaattaat caatgcaagg cccggagaaa ccctccgggc ttttttatta
    12181 tacaaagcgg caggttaggg ccttttttta taatttatag ttaaaaacgc gatataatac
    12241 agcgccgcac agcaggatcg ctgccttgac agttcatcta cattagcgtt aaaaatcccg
    12301 cagtagatga agttgtggtg gtggattaat gaccactcta aatgtttaac cggaagaagt
    12361 cagagctaat gaaaataaca atttcaggaa caggttatgt tggtctttca aatggtattc
    12421 tgattgcgca aaatcacgaa gtggttgcac tggatatcgt tcaggccaaa gtggacatgc
    12481 ttaacaagaa gcagtcaccg attgttgata aggagattga agagtatctg gcgactaaag
    12541 atctcaattt ccgcgctacg acagataagt atgacgcgta taaaaatgcc gattatgtta
    12601 ttattgccac gcctaccgat tatgacccga aaacaaatta ttttaatacc tcaagcgtgg
    12661 aagcggtcat tcgcgatgtg acagaaatta atcccaacgc ggtaatgatt ataaaatcaa
    12721 ctatccctgt tggttttaca gagtccatta aagaacggtt tggtattgaa aatgtgatct
    12781 tttcgccgga gtttttacgc gaagggaaag cactttatga taacttgcat ccatcacgca
    12841 ttgtgattgg tgagcaatcc gatcgcgctg aacgttttgc agcgttatta caggaaggcg
    12901 ctattaagca agacatacca atattgttta ctgactcaac cgaggctgag gcgattaaac
    12961 tttttgccaa tacttatctg gcgatgcgtg tggcttattt taatgaacta gatagttacg
    13021 ctgaaagtct gggtcttaac tcccgccaaa ttattgaggg ggtttgcctt gatccgcgta
    13081 tcggtaatca ctacaacaat ccctcatttg gttatggtgg ttattgtctg ccgaaagata
    13141 ccaagcagtt actggcaaac tatcaatctg taccgaataa tctgatttcg gcaattgttg
    13201 atgctaaccg cacgcgaaaa gactttattg ccgattctat ccttgcacgt aaaccgaaag
    13261 ttgttggcgt ttatcgtctg attatgaaga gtggttcaga caatttccgt gcttcctcga
    13321 ttcagggtat tatgaagcga atcaaggcga aaggtgtgcc agtaatcgtt tatgagccag
    13381 cgatgaaaga ggacgatttt ttccgttcgc gcgtggtacg tgatctggat gcgttcaaac
    13441 aagaagctga tgttattatt tctaaccgta tgtctgccga tctggctgat gtagcagata
    13501 aagtttatac gcgcgacttg tttggcaatg attaattatt ttgttttatt ctaagaaaag
    13561 gccctaatat attagggcct tttcttatgg ttttgtaaaa tcaaacttta tagaagttac
    13621 gataccattc tacaaagttc tttaccccct ctttaactga cgtttcaggt ttgaatccta
    13681 ttacgtcata cagtgctttt gtatcagcac tggtttccag tacatcaccg ggttggagag
    13741 gcatcatatt cttgttggct tcaataccca gagcctcttc taaagcattg atatagtcca
    13801 tcaactccac aggcgaacta ttaccaatgt tatagacacg atatggtgct gaacttgttg
    13861 caggcgatcc agtttctaca gtccactgtg gatctttttc tggaataaca tcctgtaagc
    13921 gaataatagc ttcggcaata tcatcaatat aagtaaagtc acgcttcatt ttgccgaagt
    13981 tgtaaacatc aatgctttta ccttccagca tggctttagt aaatttaaat aatgccatat
    14041 ccggacgtcc ccatggacca taaaccgtaa agaaacgcag ccctgtggtc ggtaagccat
    14101 acaaatgaga atacgtatgg gccatgagtt cattcgcttt tttagttgct gcataaagcg
    14161 aaacaggatg atctacagag tcatctgtag agaaaggcat cttgcggttc atgccataaa
    14221 cagaactgga ggaagcgtaa agtagatgct gaacattatt atggcggcat ccttccagta
    14281 tgttcaggaa tccaatcagg tttgcatctg catatgcatt gggattttca agggagtaac
    14341 gtacaccggc ttgcgcagcg aggtttatta cgcgatcgaa ccgctcgtct gcaaacagtg
    14401 ccgccatttt ctcgcgatcg gccaggtcaa ttttataaaa actgaaattg tcgtgcttga
    14461 gtaaatcaag tcgtgcttgt ttgaggttga catcgtaata atcatttaag ttgtcaatcc
    14521 ctacaacctg atgaccagct gcaagaagcc gtttacttac atagaaaccg ataaagccag
    14581 cagctcccgt aaccagaaat ttcatttata atcctcgctc aggctagaat atagccaatt
    14641 ttcatctggc ataactgaaa gttaaatcat accgttagac aagaaaaaaa gataatcggt
    14701 atcagttcta aacctggctg ttttttctgg taacgtgctc attttacaat caaagctgtt
    14761 ctaagctgac tatacaagcc gacgtcatta tctccaaccg tatggcagaa gagcttaagg
    14821 atgtggcaga caaagtctac acccgcgatc tttttggcag cgactaacat cttgttatca
    14881 gtgcgatttt cgccctgatt ctcttatgtt ccctttgtaa taattcatta tttttatcat
    14941 ttatcctata gcattcatgg cgattatcgc taaactatgg cgacttaaaa ttatttcgtc
    15001 cgttagggta atgatgagag tagaaaataa taatgtttct gggcaaaacc atgacccgga
    15061 acagattgat ttgattgatt tactggtgca gttgtggcgc ggcaagatga caattatcat
    15121 ctctgtcatt gttgctattg ccctagctat agggtatctg gccgttgcca aagaaaagtg
    15181 gacttcaaca gctatcgtta ctcaacctga tgtggggcaa atcgctggtt ataccaacgc
    15241 gatgaatgtg atctatggat cggctgtacc gaaagtctcc gacattcagg cttcacttat
    15301 cggacgttac agcactgcat tctcagcatt agcggaaacg ctggataacc tagaagaacc
    15361 tgaaaagctg accattgagc ctaccgtcaa aaatcagtca ttacctttag cagtttctta
    15421 tgttggtgaa acacctgaag gtgcgcagaa gcagctggcg aagtatatcc agcaagttga
    15481 cgaccaggta aatgaagaac tggaacaaga cctgaaagac aacatcgcgc tgcgtatgaa
    15541 aaacttgcag gattctctga agacgcagga agtagtcgct caagagcaga aagatctgcg
    15601 tattcgtcag attcaggaag cgttgcagta tgcgaatcag gcgcaggtga caaagccaca
    15661 gattcagcag acacaggacg tgacccaaga gactatgttc cttttaggga gcgatgcact
    15721 ggagtctatg attaagcgcg aagcgacccg tccgttggtg ttctcatcaa actactatca
    15781 gactcgtcaa aacctgctgg atatcgacaa tcttgacgtt gataaacttg atattcatgc
    15841 ttaccgttat gtgatgaagc cgacgttacc tattcgtcgc gatagtccga aaaaggcaat
    15901 caccttgatt ctggcggtgc tgctgggcgg catggttggc gcggggattg tgctggggcg
    15961 taatgctcta cgcaattaca acgcgaaata atctttgcag ctttaaagaa aacgggcagg
    16021 gtggtgactc cttgcccgtt ttttttgccg gatgcgacaa caatatcgca tccgcttacc
    16081 ccgcaactca ctgatgccgt ttacgcaggt tctcaattac cgtcgttaaa tccagatcct
    16141 gatcctgcaa cagcaccagc aggtgataca tcaagtcaga cgcctcgttg gtgagttcaa
    16201 agcggtcatg taccgttgct gctagtgcgg tttccacgcc ttcttcgccc actttctgtg
    16261 caatgcgttt ggtgccgctg gcatacagtt tggcggtgta ggaggttccc gggtcggcag
    16321 atttgcgttc ggcgagcagt tgttccagtt gatacaagaa cagccactgg tgagcggtgt
    16381 cgccgaagca gctgctggtg cctttgtggc aggtcgggcc gatgggattc gccagcaaca
    16441 gtaacgtgtc gttgtcgcag tccggcgtaa tatttactac gttgaggaaa ttgcccgacg
    16501 tttcgccttt ggtccatagt cgctgtttag tgcgcgagaa gaaggtgact ttgccgcttt
    16561 cgatggtttt gtctaaggct tccgggttca tatagcccag cattagcact tcgccggata
    16621 ccgcgtgttg cacaatcacc ggcataagtc cgtcggtttt ttcccagtcc agttcgcggc
    16681 gttgttgttc tgttaacata tcctgatctc cacgccctgt gttgccaggt acgcttttaa
    16741 ttcaccaata ttgattattt gtttgtggaa tacggaagcc gccagcgcgc cgtcaacgtc
    16801 ggcatcgcgg aaggcttcaa ggaagtgctc catagtgccc gcgccaccgg aggcaatcag
    16861 cgggacgtgg caaacttcac gcactttttt cagctgctcg aggtcgtaac cgttacgcac
    16921 gccgtcctga ttcatcatgt tgagga
//
