LOCUS       AB812058               14265 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 4370-53.
ACCESSION   AB812058
VERSION     AB812058.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 14265)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4370-53"
                     /db_xref="taxon:562"
                     /note="serogroup:O134"
     gene            <1..896
                     /gene="wcaM"
     CDS             <1..896
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01681.1"
                     /translation="LRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHI
                     IERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKH
                     FVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKY
                     LSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHL
                     FMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDID
                     RINHHIVNVEKINFRLPERRE"
     gene            1054..2049
                     /gene="gne"
     CDS             1054..2049
                     /gene="gnu"
                     /note="O134_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01682.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2292..3185
                     /gene="galF"
     CDS             2292..3185
                     /gene="galF"
                     /note="O134_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01683.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3493..4338
                     /note="O134_03"
                     /gene="welP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01684.1"
                     /translation="MINVRKIAKLIIGDNLYWKLKFFYATKKWPNIYDPTFFNEKVRW
                     RMLHDQNYLYVKCADKYEVRNYIEKKIGSEYLVPLRYMFTNNDDITDKCILSNSVAKS
                     NHGAGMIEFIPETYSLSELKNTFGKWLNLDYTSESNEKHYSRIPRKILIEESLCKEGK
                     PPVDYKFHTFKQADGSFRFVLQLVNGRFHNESRGYYLEDLNKCIWYHGEGFHSIPQEH
                     IKPLKKAMELSSILCEDFNYVRVDWYIYDNKLYFGELTFTPGAGSSYEFGNELEKLMA
                     KYWQL"
     gene            4340..5641
                     /gene="wzx"
     CDS             4340..5641
                     /gene="wzx"
                     /note="O134_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01685.1"
                     /translation="MKIIYNTLWMIFEKLVLSLSLLIINAYVARYLGPQQYGVIAYSI
                     TLLSISVVISNFGTNTLIFQKISKNHSVAKIIIPPIQILKTILYIICILIMFFCLYIF
                     SYDFDPYLTFGLAVAFYFQAIDSYTWYNDAKLKSKYNSLINTIALIGAIISRYYLVKL
                     SMDLKWFAIPYVCNYGISFLLKRYFYIKEEKTKSIKSFIFEKKDHTLKVISFFIISGA
                     PLFLSEISILIYTRLTNLYLGQIKDIDAVGIFNASYVLATAWTVVPLAFVTSVFTIIY
                     KEKDISNRALIGTFLTLLMLFFGFLLVILCYFFKQQIISIVYGSGFELSSSILPVLIL
                     GSVFSLLGVISYRMIISMHGYKYIAKKMVIMGVISIPLNYYLIKSLGIIGAAYATVSI
                     ELISATLANYFFKGGVIAKMHLKMITSPYSVSMQAITKILK"
     CDS             5643..6548
                     /note="O134_05"
                     /gene="welQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01686.1"
                     /translation="MKDQALVSIIIPTYGRCDTLNLAVQSVLKQDYKNIEVLVIDDND
                     NPIMSKKVRANLSEYQSDPRLKYFSDGVNRGGAGARNKGIELSIGYYITFLDDDDLFL
                     ENKISKQIDFLEKEQLDVCLCDMYFKQGDRFIKKSNCYANGTTLKEFILKGNCYTPMI
                     MCKRHVLIDIGGFVLTPRFQDHLLMIRILSNTSKVGHLSEQLFVHNNHNGERITYSNK
                     SAEAYILREKMEREYIYLLNSNERKTFEFKKALIRMKIYRLKNEHMKLLKITLYAMKN
                     LTNFSDVYALGKTLIRVMFFGSKNV"
     gene            6545..7684
                     /gene="wzy"
     CDS             6545..7684
                     /gene="wzy"
                     /note="O134_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01687.1"
                     /translation="MKTNNKKIKALIFFISIFLYFFKSVFISLELPEDLIGGVSNIIN
                     ALILAILSPVILLSNKKQCAFIIFLIILFVYNAIFYNNIYMFGFIIIACMLILSNGIY
                     WRDIIRYLSLAHFLVFLFIIPLVFFAEKYSFIDDRFGVRYTFGFHNPNTFSQYLISLY
                     VVFLLLFVEVIKKKSLQFLTVIILTLFIYGVISLTGSRTGMILTIITGFGFLACTLSK
                     TNDKKRRKFVYLYILGAGILCFIQYYLALTYNHSEFSKNINTILSGRIWFSSQLTSQL
                     WPVPYFHGVNINDYLPIDFFYVAYFYNLGIFIGSWFIYLFIRKMFVQTYTPVMVIALW
                     LSLAITVTENYYAIPLYNISLFIVFSSRYVINENENSCTYPSSQK"
     CDS             7701..8474
                     /note="O134_07"
                     /gene="welR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01688.1"
                     /translation="MALNNPSVRFYVHMDAKYPNKIQWIKNECIDNIYLIENPVSVYW
                     GGSSQIFATLLLMKKAYSDKRNKFFHLVSSECVPLKSFVEIENEWSMNENCQFIESHR
                     DKNNEWRLKVRVPHSNTKYLRTFLGRCANKLFKVTTFLWDSVGLNPENYFFGSQWFSV
                     TRNFVEQVIDEDNEDFFKTFHNITCADEHAFAIFARTKYSNPNDIQDNNKRFIKFLGK
                     SSPEYLSLDQVGQIKNLNSYWFCRKVRCNDLLKIIRQEK"
     CDS             8471..9205
                     /note="O134_08"
                     /gene="wbdN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01689.1"
                     /translation="MIMLFSIIMPAYNSQATIKESIASVLNQTYQNFELIIINDNSSD
                     ATLSIITNFCHDRRILVLNNEENMGVAHSRNRGLEMASGEIIAFLDSDDIWYPNKLEE
                     QYNCFLSGHKIVCSYYDVIDSEGNIVGTRNAPTLVTFEKMLKSNFIGNLTGAYASSFF
                     GKCYQRNIGHEDYIMWLELVKKQPAYCIKNKLAAYRISNKSLSSNKMKVVIWQWKIYR
                     KVLGMNIIKSLYYFLNYICFAIKKRN"
     gene            9324..10730
                     /gene="gnd"
     CDS             9324..10730
                     /gene="gnd"
                     /note="O134_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01690.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10979..12145
                     /gene="ugd"
     CDS             10979..12145
                     /gene="ugd"
                     /note="O134_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01691.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            12235..13269
                     /gene="wzz"
     CDS             12235..13269
                     /gene="wzz"
                     /note="O134_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01692.1"
                     /translation="MAIIAKLWRLQIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVQLGCMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(13365..13976)
                     /gene="hisI"
     CDS             complement(13365..13976)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01693.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4287 a   2635 c   3045 g   4298 t
ORIGIN
        1 tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat tgtaagttca
       61 gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt gatattttga
      121 tctccgacca catcatcgag cgcatcaact gtactaacgg caaaatcaac tggggcattg
      181 gcataggtct tgcgggaagc acttatgata ataactaccc ggaagaccag gcagtgaaaa
      241 actttgtcgt ggcgaatatc acggggtcgg attgtcggca gttgatccat gttgaaaatg
      301 gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat ttcagtaaga
      361 aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc gtgattgata
      421 atattgaaat gattaatagc gccgggatgt taattggcta tggggtaatt aaaggcaaat
      481 atctctcgat accgcaaaat ttccgagtga ataatattca actggataac acccatcttg
      541 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtggcactga
      601 ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa catcttttta
      661 tgcgaaatat caaggtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
      721 acatgcgcaa agacgtacgc ggtgtcttta tggcgaaaaa agaaacactg ctgtcacttg
      781 caaatgttca tgcgatgaat gaaagagggc aaagctccgt cgacatcgac aggattaatc
      841 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggaga gagtagattt
      901 gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc tcacactaac
      961 tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc tttatcttgt
     1021 gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt gctcatagga
     1081 gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt taatatcaag
     1141 aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg tgatgttcgt
     1201 gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtgct actggcagcg
     1261 gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt tcagggtacc
     1321 cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt taccagttcc
     1381 gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca cgaccctttc
     1441 aaccactacg gcaaaagtaa gtggcaggca gaggaagtgc tgcgtgaatg gtataacaaa
     1501 gcaccaacag aacgttcatt aaccatcatc cgtcctaccg ttatcttcgg tgaacggaac
     1561 cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat gatggtgggc
     1621 gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt tatcaagtac
     1681 aaactgaaga atgttgccgc aggttatgag gtttataact acgttgataa gccagacctg
     1741 aacatgaacc agttggttgc tgaagttgaa caaagcctga acaaaaagat cccttctatg
     1801 cacttgcctt acccactagg aatgctgggg ggctattgtt ttgatatcct gagcaaaatt
     1861 acgggcaaaa aatacgctgt cagctcagtg cgcgtgaaaa aattctgcgc aacaacacag
     1921 tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct gtcgcaaggt
     1981 ctggatcgaa ctctgcagta tgaattcgtt catgccaaaa aagacgacat aacgtttgtt
     2041 tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga ttattcggaa
     2101 agaggtactg attaaattat cagtatgcgg aattccgagt aatccgagta acccagatgc
     2161 tgatggtgtg tagtgttatc ctaaggttta agcaaagtta tgcggaacta agctttggta
     2221 tatagccata acgtttggct aacctatggc aataatttac gcactatcag ccacttagag
     2281 gtaggaaaag aatgacgaat ttaaaagcag ttataccggt agcaggtctt gggatgcata
     2341 tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac aagccaatga
     2401 ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc ctggtaactc
     2461 atgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta gaatctctcc
     2521 ttgaacagcg cgtgaagcgt caactactgg cggaagtaca atctatctgt ccgccgggcg
     2581 tgaccattat gaacgtgcgt cagggtgaac ctttaggttt aggccactcc attttatgtg
     2641 cgcgacctgc cattggtgac aatccatttg tcgtggtgct gccagacgtt gtgatcgacg
     2701 acgccagcgc cgacccgcta cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa
     2761 cgggccgtag ccaggtgctg gcaaaacgta tgccgggtga gctctctgaa tactcagtca
     2821 ttcagacaaa agaaccactg gatcgtgaag gtaaagtcag ccgcattgtt gaattcatcg
     2881 aaaagccgga tcagccgcag acgctggact cagacatcat ggccgttggt cgctatgtgc
     2941 tttctgccga tatttggccg gaacttgaac gtactcagcc tggtgcatgg ggacgtattc
     3001 agctgactga tgccattgcc gagctggcga aaaaacagtc cgttgatgcc atgctgatga
     3061 ccggcgacag ctacgactgc ggtaaaaaaa tgggttatat gcaggcgttt gtgaagtatg
     3121 gactacgcaa cctgaaagaa ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg
     3181 aataatgaaa atctaaccga atgtaacggt tgacaagaaa attataacgg cggtgaagat
     3241 tcgtggcgaa agtaatttgt tgcgaatatt cctgccgtag ttttatataa acaatcagag
     3301 taacaacgag ttagcaatag gattttaatc aaagttttcc aggattttcc ttgtttccag
     3361 agctgattgg taagacaatt agcgtttgaa tttttcgggt ttagcgcgag tgggtaatgc
     3421 tcgtcacatc gtaggcatgc atgcagtgct ctggtagctg taaagccagg ggcggtagct
     3481 tggtaaaaaa tcatgataaa tgtaagaaaa atagcaaaat taattattgg cgataatttg
     3541 tattggaagt tgaagttttt ttatgccact aaaaaatggc ctaatattta tgatccaact
     3601 ttttttaatg aaaaagttcg ctggcgaatg ctgcacgacc aaaattatct ttatgttaaa
     3661 tgcgctgata agtatgaggt caggaactat attgagaaaa aaattggcag tgaatattta
     3721 gttccgcttc gttatatgtt tactaataat gatgatatta ctgataaatg tattctgagt
     3781 aatagtgtag caaagtctaa ccatggtgca ggtatgatag agttcattcc tgagacgtat
     3841 tcattaagcg agttgaagaa tacttttgga aaatggctaa atcttgacta tacctccgag
     3901 tccaatgaaa aacattatag taggattccg agaaaaattc ttattgaaga gtctttgtgt
     3961 aaggagggaa aacctcctgt cgattataaa tttcatacat ttaaacaagc agatggttca
     4021 tttcgttttg ttcttcagtt ggtaaatggt cggtttcata atgaatcaag aggttattat
     4081 ctggaggatc ttaataaatg catatggtat catggtgaag gttttcattc tattccacaa
     4141 gagcatataa aacctttaaa aaaggctatg gaattgtcat caattctatg tgaagatttt
     4201 aattacgtac gagttgattg gtatatctat gataataagc tttattttgg tgaattaacc
     4261 tttacacctg gtgctggaag ttcatatgaa tttggtaatg agcttgaaaa attaatggcc
     4321 aaatattggc agttgtaaaa tgaaaataat atataacaca ctttggatga tatttgaaaa
     4381 attagtatta tccctatctt tattaatcat taatgcctat gttgcgagat atttagggcc
     4441 tcagcaatat ggggttatag cctattctat tacactgctc agtatttctg tggttattag
     4501 taattttggc actaacacat taattttcca aaaaataagc aaaaatcaca gtgttgcaaa
     4561 aatcattata ccgccgatac agatattgaa aacaatatta tacataatat gtatacttat
     4621 tatgtttttc tgtttatata tttttagtta tgattttgat ccttatctga catttggttt
     4681 agctgtagca ttttactttc aagctattga tagctataca tggtataatg atgcaaaatt
     4741 gaaatcaaag tacaatagtc ttattaatac aatagcctta ataggtgcta ttataagtcg
     4801 ttactatttg gtaaaactat cgatggattt aaaatggttt gctattcctt atgtatgcaa
     4861 ttacgggata tcttttttac tgaaaagata tttttatatt aaagaagaaa aaactaaaag
     4921 catcaaatca tttatatttg aaaaaaaaga tcatacacta aaagttatat ctttctttat
     4981 aatttcaggt gcgccattat ttttaagtga aatatcaatt cttatttata caagacttac
     5041 aaacttatat ctaggacaaa ttaaagatat tgatgcggtc ggcatattca atgcatctta
     5101 tgttttagct actgcctgga cagtcgttcc tttggcattt gttacgagtg tctttactat
     5161 aatctataaa gaaaaggata tttctaacag ggctttaatt ggaacattct tgactttgtt
     5221 gatgttgttt tttggttttt tacttgtcat attgtgttat tttttcaaac aacagattat
     5281 cagtattgta tatggctccg gtttcgagtt atcttcttct atattacctg tgcttattct
     5341 tggctcagtt ttctctcttt taggtgttat ttcctatcgg atgataatat caatgcatgg
     5401 gtacaaatat atagcaaaaa agatggttat tatgggggtg ataagtattc cattaaatta
     5461 ttatcttatt aaatcgttag gtattattgg tgcagcttat gctacagtta gtattgaact
     5521 tatatcagcc acattggcga attatttttt taaaggtggg gtgattgcaa aaatgcattt
     5581 aaaaatgata acgtctccct acagtgtatc tatgcaagct ataactaaaa tactgaaata
     5641 aaatgaaaga tcaagcatta gtctctatta ttattccaac atatgggcgg tgtgacactt
     5701 taaatcttgc tgtccaaagt gtactaaaac aggattataa aaacatagaa gtattggtta
     5761 ttgatgataa tgataatcca ataatgtcta agaaagttcg tgccaattta tcggaatatc
     5821 aatccgatcc cagattaaag tattttagtg atggggttaa tagaggtgga gcaggggcaa
     5881 gaaataaagg aattgaattg tcaataggtt attatattac ttttcttgat gacgatgatt
     5941 tgtttttaga aaataaaata tcaaagcaaa ttgacttttt agaaaaagaa caacttgatg
     6001 tatgcctttg tgatatgtat tttaaacaag gagaccgttt tataaaaaaa tcaaattgct
     6061 atgcaaatgg aacaacatta aaagaattta tactcaaggg aaattgttac acgccaatga
     6121 tcatgtgtaa acgacatgtt ctaattgata ttggtggatt cgttttaact cctcggtttc
     6181 aagatcactt attaatgatt aggattcttt ctaatacttc gaaagtgggg catttatctg
     6241 agcaattatt tgttcataat aaccataatg gtgaaaggat tacatatagt aataaatctg
     6301 ctgaagcata tatattacgc gaaaagatgg agcgagaata tatttattta ttaaattcaa
     6361 atgaacgtaa aacattcgaa ttcaagaagg cacttataag gatgaaaata tatagattaa
     6421 aaaatgaaca tatgaagtta cttaaaataa cattatatgc catgaaaaac ctcactaact
     6481 tttcagatgt ttacgcatta ggaaaaactt taataagagt gatgtttttt gggagtaaaa
     6541 atgtgtgaaa acaaataaca aaaaaataaa agcattgatt ttttttatat caatatttct
     6601 ctactttttc aaaagtgttt ttatttcatt agagcttcct gaggatttaa tagggggagt
     6661 atcaaatatc ataaatgcac taatattggc tatattatct ccagtcattc tactgtcaaa
     6721 taaaaaacaa tgtgcattta taattttttt aattatattg tttgtttata atgccatttt
     6781 ttataataac atctatatgt ttggttttat aattattgca tgcatgttga tactaagtaa
     6841 cggaatatat tggagggata ttatcagata cttgtcactg gcccattttt tggtgtttct
     6901 ttttatcatt cctttagttt tttttgcaga aaaatactct tttattgatg atagatttgg
     6961 agttagatat acatttggct tccataatcc gaatacattt agtcaatatt taatttcttt
     7021 atatgtagtg ttcttgttgt tatttgttga agttatcaaa aaaaaatctc tgcaattttt
     7081 gactgttata atattgactc tatttatata tggggtgata tccttaactg gttcaaggac
     7141 gggaatgata ttaactatta ttaccggctt cggtttttta gcatgtacac tgtctaaaac
     7201 gaatgataaa aaaagaagaa agttcgtata tttatacata ctaggtgcag gaatattatg
     7261 ttttatacaa tattatcttg ccttaacata taatcattca gagttctcaa aaaacataaa
     7321 tacaattctt tctggtagaa tatggttttc ttcacaatta actagccaat tatggcctgt
     7381 cccatatttc catggtgtga atattaatga ttatttgcca attgatttct tctacgtggc
     7441 ttatttttac aatcttggga tttttattgg ttcttggttt atatacctct ttataagaaa
     7501 aatgtttgtg caaacatata ctcctgttat ggtaattgcg ctttggttat cgcttgcaat
     7561 aacggtcact gaaaattatt atgcaattcc gttgtataat attagtttat ttattgtttt
     7621 ttcctcaaga tatgtgatta atgaaaatga aaacagctgt acttatccaa gctcacaaaa
     7681 atgaaaatta tattagagaa ttggcgttaa acaatccatc agttcgtttt tatgtacata
     7741 tggatgccaa gtatccaaat aaaatccaat ggattaaaaa tgaatgtatt gataacatct
     7801 atttaattga aaatccagtt tcggtttact gggggggaag tagtcagatt tttgcaactc
     7861 tattattgat gaagaaggca tacagtgata agagaaataa gttttttcat ctcgtaagtt
     7921 cagaatgcgt tccattaaag tcatttgttg aaattgaaaa cgaatggagc atgaatgaaa
     7981 actgtcaatt tattgagtcg catagagata aaaataatga gtggagacta aaggttcggg
     8041 tccctcactc aaatacgaag tatcttagga cttttttagg acgatgtgcc aataaactct
     8101 ttaaagtaac tactttttta tgggatagtg taggtttaaa tcccgaaaac tatttttttg
     8161 gttcccaatg gttttcggta actagaaatt ttgttgagca agtaatagat gaagataatg
     8221 aagatttttt taaaacattc cataatataa cttgtgcaga tgagcatgct tttgctattt
     8281 ttgcacgaac aaaatattct aacccaaatg atattcaaga taacaataaa agattcatta
     8341 agtttttagg taagtcaagt ccagaatatt taagtttaga tcaagtgggg cagattaaga
     8401 atcttaactc gtattggttt tgcaggaagg tgagatgtaa tgatttatta aaaataataa
     8461 ggcaggagaa gtgattatgc ttttttctat cattatgccg gcatataatt cacaggcaac
     8521 aataaaagaa agtatagcat ccgtgcttaa tcagacatat caaaattttg aattaattat
     8581 aattaatgat aattcaagtg acgcaacttt atcgataata acaaatttct gtcatgatag
     8641 aaggattctt gtattaaata atgaggaaaa tatgggagtg gcacactctc gtaacagagg
     8701 attggaaatg gcatctggag aaataattgc ctttctggat agtgacgata tatggtaccc
     8761 caataaattg gaagagcaat ataattgttt tctttctggt cacaaaattg tctgctctta
     8821 ttatgatgta attgactctg aagggaatat tgttggaaca agaaatgcac ctacactcgt
     8881 tacatttgaa aaaatgctta aaagtaattt tataggtaac ttgactggtg catatgcctc
     8941 ctcttttttt ggaaaatgtt atcagagaaa tattgggcat gaggattata ttatgtggtt
     9001 ggaattggta aaaaaacaac ctgcttattg cattaaaaat aaactagccg cataccgaat
     9061 ttcaaataaa tctctttctt ctaataaaat gaaagtggta atttggcaat ggaaaatata
     9121 tcgtaaagta ttaggtatga atattatcaa gtctttatat tacttcttga attatatatg
     9181 ttttgcgata aaaaaaagaa actaatattc aataagtttt aatttttttt tcattcaatt
     9241 tcagtaataa ttgtagttat ttgcgtatag ttatacccta accgaacata cccgcagaca
     9301 acaccccctg acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag
     9361 tgatggggcg caaccttgcg ctcaatatcg aaagccgtgg ttataccgtc tctattttca
     9421 accgttcccg tgaaaagacg gaagaagtaa ttgctgaaaa tccaggcaag aaactggttc
     9481 cttactatac ggtgaaagag tttgttgaat ctctggaaac gcctcgtcgc atcctgttaa
     9541 tggtgaaagc aggtgcaggc acggatgctg ctattgattc cctcaagcca tacctcgata
     9601 aaggtgacat catcattgat ggtggtaata ccttcttcca ggacaccatt cgtcgtaatc
     9661 gtgagctttc tgcagaaggc tttaacttca ttggtaccgg tgtttccggc ggtgaagaag
     9721 gtgcgctgaa aggtccttcc attatgcctg gtgggcagaa agaagcctat gaactggttg
     9781 caccgatcct gaccaaaatc gccgcagtgg ctgaagacgg cgaaccgtgc gttacctata
     9841 ttggtgccga tggcgcaggt cactatgtga agatggttca caacggtatt gaatacggtg
     9901 atatgcagct gattgctgaa gcctattctc tgctaaaagg tggcctgaac ctcaccaacg
     9961 aagaactggc acagaccttt accgagtgga ataacggtga actgagcagc tacctgatcg
    10021 acatcaccaa agatatcttc accaaaaaag atgaagacgg taactacctg gttgatgtga
    10081 ttctggatga agcggctaac aaaggtaccg gtaaatggac cagccagagt gcgctggatc
    10141 tcggcgaacc gctgtcgctg attaccgagt ctgtgtttgc acgttatatc tcttctctga
    10201 aagatcagcg cgttgccgcg tctaaagttc tctctggccc gcaagcgcag ccagcaggcg
    10261 acaaggctga gttcatcgaa aaagttcgcc gtgcgctgta tctgggcaaa atcgtttctt
    10321 acgctcaggg cttctctcag ctacgcgccg cgtctgaaga gtacaactgg gatctgaact
    10381 acggtgaaat cgcgaagatt ttccgtgctg gctgcatcat ccgtgcgcag ttcctgcaga
    10441 aaatcaccga tgcatatgcc gaaaatccgc agatcgctaa cctgctgctg gctccgtact
    10501 tcaagcaaat cgccgatgac taccagcagg cgctgcgcga tgtcgtcgct tacgcggtac
    10561 agaacggtat cccggttccg accttcgccg ctgcggttgc ctattatgac agctaccgcg
    10621 ccgcagttct gcctgcaaac ctgatccagg cacagcgtga ctatttcggt gcgcatactt
    10681 ataagcgcat tgataaagaa ggtgtgttcc atactgaatg gctggattaa tctgatttaa
    10741 atcaattaat caatgcaagg cccggagaaa ccctctgggc ttttttatta tacaaagcgg
    10801 caggttatgg ccttttttta taatttatag ttaaaaacgc gatataatac agcgccgcac
    10861 agcaggatcg ctgccttgac agttcatcta catcagcgtt aaaaatcccg cagtagatga
    10921 agttgtggtg gtggattaat gaccactcta aatgtttaac cggaagaagt cagagctaat
    10981 gaaaataaca atttcaggaa caggttatgt tggtctttca aatggtattc tgattgcgca
    11041 aaatcacgaa gtggttgcac tggatatcgt tcaggccaaa gtggacatgc ttaacaagaa
    11101 gcagtcaccg attgttgata aggagattga agagtatctg gcgactaaag atctcaattt
    11161 ccgcgctacg acagataagt atgacgcgta taaaaatgcc gattacgtta ttattgccac
    11221 gcctaccgat tatgacccga aaacaaatta ttttaatacc tcaagcgtgg aagcggtcat
    11281 tcgcgatgtg acagaaatta atcctaatgc tgtcatggtg attaaatcta caattccggt
    11341 aggtttcacc aaatcaatta aagaacgtct gggtatcgat aaccttattt tctctccgga
    11401 attcctccgc gaaggtaaag ctctttacga taatctccat ccgtcacgta ttgtcatcgg
    11461 tgagcgttca gaacgcgcag aacgttttgc tgcgttgtta caggaagggg cgattaagca
    11521 aaatatccct acattgttta ccgactccac tgaagcagaa gcgattaaac tttttgctaa
    11581 tacttatctg gcgatgcgcg tagcatactt taatgaactg gatagctatg cagaaagttt
    11641 agggctgaat actcgccaga ttatcgaagg cgtttgtctc gatccgcgta ttggcaacca
    11701 ttacaacaat ccttcgtttg gttatggtgg ttattgtctg ccgaaagata ccaagcagtt
    11761 actggcgaac taccagtctg tgccgaataa cctgatttcg gcaattgtcg atgctaaccg
    11821 cacacgtaaa gactttattg ccgatgccat tttgtcacgc aaaccgcaag tggtgggtat
    11881 ttatcgtctg attatgaaga gcgggtcaga taacttccgt gcgtcttcca ttcaggggat
    11941 tatgaaacgt atcaaggcga aaggcgttga agtgatcatc tacgaaccgg tgatgaaaga
    12001 agactcattc ttcaactctc gcctggaacg tgatctcgcc accttcaaac aacaagccga
    12061 cgtcattatc tccaaccgta tggcagaaga gcttaaggat gtggcagaca aagtctacac
    12121 ccgcgatctc tttggcagtg actaacatct tgttatcagg gcgattttcg ccctgattct
    12181 cttatgctcc ctttgtaata attcattatt tttatcattt atcctatagc attcatggcg
    12241 attatcgcta aactatggcg acttcaaatt atttcgtccg ttagggtaat gatgagagta
    12301 gaaaataata atgtttctgg gcaaaaccat gacccggaac agattgattt gattgattta
    12361 ctggtgcagt tgtggcgtgg caagatgaca attatcatct ctgtcattgt cgctattgcc
    12421 ctggctatag ggtatctggc cgttgccaaa gaaaagtgga cttctacagc tatcgttact
    12481 caacctgatg tggggcaaat cgctggttat aacaatgcca taaatgtgat ttatggttcg
    12541 gcagcgccga aagtgtctga gatccagtcg atgttaattg gtcgtttcag cacgacattt
    12601 tctgcattag cagaaacgct ggataatcag gaagaacctg aaaaactgac cattgagcct
    12661 accgttaaaa atcagtcatt acctttagcg gtttcttatg tgggtgaaac acctgaaggt
    12721 gcgcagaaac agctggcgca gtatatccag caagttgacg accaggtaaa tgaagaactg
    12781 gaacaagacc tgaaagacaa catcgcgctg caaatgaaaa acttgcagga ttctctgaag
    12841 acccaggaag tggttgcgca ggagcagaaa gatctgcgta tccgtcagat tcaggaagcg
    12901 ttgcagtatg cgaatcaggc gcaggtgaca aagccgcaga ttcagcagac acaggacgta
    12961 acccaggata ctatgttcct tttggggagc gaagcgctgg agtcgatgat taagcacgag
    13021 gcgactcgtc cgttggtgtt ctcatcaaac tactatcaga ctcgtcaaaa cctgctggat
    13081 atcgacaatc ttgacgttga taaacttgat attcatgctt accgctatgt aatgaaaccg
    13141 acgttaccta ttcgtcgcga tagcccgaaa aaggcaatta ccttgattct ggcggtgcag
    13201 ctgggctgca tggttggcgc ggggattgtg ttggggcgta acgctctgcg taattacaac
    13261 gcgaagtaat cttttcggtt ttaaagaaaa tgggcagggt ggtgacacct tgcccgtttt
    13321 tttgccggat gcgacaacaa catcgcatcc gcttaccccg caactcactg atgccgttta
    13381 cgcaggttct caatcaccgt cgttaagtcc agatcctgat cctgcaacag caccagcagg
    13441 tgatacatca agtcagatgc ctcgttggtc agctcaaagc ggtcatgtac agtcgcggcc
    13501 agtgcggttt ccacgccttc ttcgcccact ttctgcgcaa tgcgtttggt gccgctggca
    13561 tacagtttgg cagtgtaaga ggtttccggg tcggcagatt tgcgttcggc gagcagttgt
    13621 tccagttgat ataggaacag ccactggtga gcggtatcgc cgaagcagct gctggtgcct
    13681 ttgtggcagg ttggcccgat gggattcgcc agcaccagta aagtgtcgtt gtcgcagtcc
    13741 ggggcaatac tcaccacatt gagaaaattg cccgacgttt cgcctttggt ccacagtcgc
    13801 tgtttagtgc gcgagaagaa ggtgactttg ccgctttcga tggttttgtc taaggcttcc
    13861 gggttcatat agcccagcat tagcacttcg ccggataccg cgtgttgcac aatcaccggc
    13921 atcagtccgt cggttttttc ccagtccagt tcgcgacgtt gttgttctgt taacatatcc
    13981 tgatctccac gccctgtgtt gccaggtacg cttttaattc accaatattg attatttgtt
    14041 tgtggaatac ggaagccgcc agcgcgccgt caacgtcggc atcgcggaag gcttcgagga
    14101 agtgttccat ggtgcccgcg ccaccggagg caatcagcgg gacgtggcaa acttcacgca
    14161 cttttttcag ttgttcgagg tcgtaaccgt tacgcacgcc gtcctgattc atcatgttga
    14221 ggacgatttc tccggcaccg cgtttttgca cttcctgtac ccagt
//
