LOCUS       EU296423               11364 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O135 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296423
VERSION     EU296423.1  GI:187880664
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O135"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACD37166.1"
                     /db_xref="GI:187880665"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     misc_feature    1138..8868
                     /note="O-antigen gene cluster"
     gene            2223..3122
                     /gene="rmlD"
     CDS             2223..3122
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACD37167.1"
                     /db_xref="GI:187880666"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPYNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3181..4059
                     /gene="rmlA"
     CDS             3181..4059
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACD37168.1"
                     /db_xref="GI:187880667"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4064..4609
                     /gene="rmlC"
     CDS             4064..4609
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACD37169.1"
                     /db_xref="GI:187880668"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            4625..5881
                     /gene="wzx"
     CDS             4625..5881
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37170.1"
                     /db_xref="GI:187880669"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLSFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFIFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            5911..6801
                     /gene="rfbF"
     CDS             5911..6801
                     /gene="wfgI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgI"
                     /protein_id="ACD37171.1"
                     /db_xref="GI:187880670"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            6794..7699
                     /gene="rfbG"
     CDS             6794..7699
                     /gene="wfgK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgK"
                     /protein_id="ACD37172.1"
                     /db_xref="GI:187880671"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQVSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            7720..8868
                     /gene="wzy"
     CDS             7720..8868
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37173.1"
                     /db_xref="GI:187880672"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKLFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFVKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
BASE COUNT     3336 a   1992 c   2481 g   3555 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacccctc ttatgaatta gaatctctcc ttgaacagcg cgtaaagcgt
      121 caactattgg cggaagtaca gtccatttgc ccgccgggcg tgaccattat gaacgtgcga
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacggcctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactctgtca tccagaccaa agagccgctg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc aggtgcatgg gggcgtattc agctgactga tgccatcgct
      601 gaactgggga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaagcgttc gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ttgttaagcg aataatgaaa atctcaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcata agttttgctc
      841 attgaatttt cctgccgttg ccgtttttat aaagcctata aaaacaagaa gttacatagc
      901 tcatattatt caaatttttc caggattttc cttgtttcta cggctgtttg gtaagacaat
      961 tagcgtttga atttttcgga tttggcgcga gtggctaacg ctcgccacat cgttgagatg
     1021 catgcagtgc actggtagct gttaagccag gggcggtagc gtgtattaat acctctatta
     1081 atcaaatctg gagcagtcta tttcacagca tgctctatag ctatatggaa taaaaaagtg
     1141 aagatacttg ttactggtgg cgcagggttt attggttctg ctgttgttcg tcacataata
     1201 aataatacgc aagatagtgt tgttaatgtc gataaattaa catacgccgg gaacctggaa
     1261 tcactcgctg atgtttctga ttctgaacgt tatgcttttg agcatgcaga tatctgcgat
     1321 gctgtagcca tgtcgcgtat tttcgcacag caccagccag acgcggtgat gcacctggcg
     1381 gcagagagcc acgttgaccg ttcaataacg ggccctgcgg catttattga aaccaatatt
     1441 gtggggactt atgttctttt agaagcggcg cgcaattact ggtctgctct gaatgatgaa
     1501 aagaaaaaaa gtttccgctt tcatcatatt tctactgatg aagtttatgg tgacttaccc
     1561 catccggatg aagcaaataa taacgaagcg ttaccgctat ttacggaaac gacagcttac
     1621 gcgccaagta gcccgtattc tgcgtctaaa gcttccagcg atcatttggt acgtgcctgg
     1681 aagcggactt atggtttacc aaccattgtg actaattgct cgaacaatta tggtccttat
     1741 catttcccgg aaaaacttat tccattggtt attctgaatg ctctggaagg taaggcatta
     1801 cctatttatg gtaaagggga tcaaattcgc gactggttgt atgtagagga tcatgctcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacat
     1921 aacgaaaaga aaaacatcga cgtagtgctc acgatttgtg atttgttgga tgagattgta
     1981 ccgaaagaga aatcttatcg tgagcaaatt acttatgttg ccgatcgccc ggggcatgac
     2041 cgccgttacg ccattgatgc cgataaaatt agccgcgaat tgggctggaa accgcaagaa
     2101 acgtttgaga gcgggattcg taaaacggtg gaatggtatc tggctaatac aaattgggtt
     2161 gagaatgtga aaagcggtgc ttatcagtcg tggattgaac aaaactatga gggccgtcag
     2221 taatgaatat cctgcttttc ggcaaaacag ggcaggtggg ttgggaactg cagcgtgctc
     2281 tggctcctct gggtaacttg attgcgctcg atgttcactc cactgattat tgtggagatt
     2341 tcagcaaccc cgaaggtgtg gctgaaaccg tcaaaaaaat tcgccccgat gttattgtta
     2401 atgctgctgc tcacactgca gtagacaaag cggaatcaga accgaaattc gcgcaattac
     2461 ttaacgcgac aagcgtcgaa gcgattgcaa aagcagctaa tgaagtcgga gcctgggtta
     2521 tacactactc cactgattat gttttcccag gcaacggtga cacgccatgg ctggaaacgg
     2581 atgcaacagc accgctaaat gtctacggtg aaaccaagct agctggggaa aaagcgttac
     2641 aagaacattg cgcaaagcat cttattttcc gtaccagctg ggtatacgct ggtaaaggaa
     2701 ataactttgc caaaacgatg ttgcgcctgg caaaagaacg cgaagagttg gctgtaataa
     2761 acgatcaatt cggcgcacca acgggggctg aattactggc tgattgcacc gctcatgcca
     2821 ttcgcgtggc cgcaaataaa ccagaagttg ctggtttgta ccatctggtt gctggtggta
     2881 caacaacctg gcacgattat gccgctctgg tatttgaaga agcgcgcaga gcggggatca
     2941 accttgctct taacaaactt aatgccgtgc cgacaacggc ctatcccaca ccagcacgtc
     3001 gtccctataa ttctcgcctc aataccgaaa aatttcagca gaactttgcg cttgtcttgc
     3061 ctgactggca ggtgggcgtg aaacgtatgc tcaacgaatt atttacgacc acggcaattt
     3121 aacaaattat tgcatctcgc tcatgatgcc agagcgagat gaattaaaag gaatggtgaa
     3181 atgaaaacgc gtaaaggtat tattctggct ggtggttccg gcactcgtct ttatcctgtg
     3241 actatggctg tgagcaaaca actgctaccg atttatgata aaccaatgat ttattatccg
     3301 ctttcaacgc ttatgttggc gggtattcga gatattctga ttatcagtac gccgcaggat
     3361 acaccacgtt ttcaacaatt gcttggtgat ggaagccagt gggggcttaa tctgcaatat
     3421 aaagtacaac cgagtccgga tggtctggcg caagcattta tcattggtga agaatttata
     3481 ggtggtgatg attgtgcgct ggtacttggt gataatatct tctatggtca cgacctgccg
     3541 aaattaatgg ataccgctgt taacagagag agtggcgcaa cggtttttgc ctatcacgta
     3601 aatgatccag agcgctacgg tgtggtggaa tttgatgata acggtacggc tattagtctg
     3661 gaagaaaaac cgctggaacc aaaaagtaac tatgcggtta ctgggcttta tttctatgac
     3721 aatgacgttg tagaaatggc gaaaaacctt aaaccatctg cacgtggcga actggaaatt
     3781 actgatatta accgtattta tatggaacaa ggacgtttgt ctgtcgctat gatggggcgt
     3841 ggttatgcat ggctggacac ggggacacat caaagtctta ttgaagcaag caacttcatt
     3901 gcaacaattg aagagcgtca ggggctgaaa gtttcctgcc cggaagaaat tgcttaccgt
     3961 aaaggattta ttgatgctga gcaagtgaag gtattagccg agccgctgaa gaaaaatgct
     4021 tatggtcagt atctgctgaa aatgattaaa ggttattaat aaaatgaacg taattaaaac
     4081 tgaaattcct gatgtgctaa tttttgaacc aaaagtattt ggtgatgagc ggggcttttt
     4141 tatggaaagc ttcaatcaga aagttttcga agaggctgta ggacgaaagg ttgaatttgt
     4201 tcaggataac cattctaaat caactaaggg tgtgttacgc ggactgcact atcagctgga
     4261 accttatgct caaggaaaac tggtacgctg tgttgtcggt gaagtttttg acgtagctgt
     4321 tgatattcgg aaatcatcac caacatttgg caagtgggtt ggtgtaaatt tgtcggcgga
     4381 gaataagcgt caattatgga taccggaagg attcgctcat gggttttgcg ttcttagtga
     4441 tgaagcagaa tttgtctaca aaacaaataa tttctattca aaaatgcaag agcgtgggat
     4501 tttatggagc gacaaaagta taaatataga atggccggtt caaaatccat tgctttctga
     4561 taaagatatt aatggtcaaa aatttgtaga tgctgattat tttatatgat aaagaaatgt
     4621 aataatgagc ataataaaaa atagtgtctg gaaccttttt ggctatgcaa taccaacttt
     4681 aattgctatc ccctcgctag gatttctcgc tcgaggtttg gggcctgaag gtttcggtgt
     4741 ttatacaatt gcaattgcac ttgttgggta tgctggaatt tttgatgtag gcctgactcg
     4801 ctctgttatt agagaaattg caattcatcg agataatcat catgaaagaa ccaaggtaat
     4861 ttcaacaagt acatcttttt tggtgctatt ttcatgcttt ggtgcttttt tattattgat
     4921 tttctctgat ggaattgtta attatctaaa aatttctggt gttgaacata gtgatataca
     4981 actatcattt aaactgttgg ctatttgcat tccattattt attctaaatc aattatggtc
     5041 agccattctt gagggggatg aaaaatttgg cattgtaaat attcaaaaat ctatatcaag
     5101 ctcttgcatc gcgggaattc cggccatatt tgttttttat agtgctacat tgtcggcagc
     5161 ggttgctggt ttaatatttg caagagttat ttcgatttta gtctctgcct attatgtcag
     5221 gaatgatatt aaaatttcgg gggttcattt ttgttataaa acctttaaac gactcttttt
     5281 ctttggcggt tggatgacag taagtaatat cataagtccg gtcatggtgt attttgatcg
     5341 atttatagtg tcaaatatta tgggggcaga taaagttgca ttttattctg caccagcgga
     5401 ggttattcta aaattaggaa taatacctgc agcaatcggg agagcagtgt ttccaaggtt
     5461 aagtaacatc aaagacttta aagaatttaa gcgtaatgta aataaatcat tgcttttaat
     5521 gtttctaatc tgtttgccgg tgataatcat aggcttgtta tattcagggc ttgtattgaa
     5581 aatatggttt ggtgagaatt accaaattaa ttcctttaat atattaaatg tgttattgat
     5641 cggttttatc ttcaatgcgc tggcaatgat accattctct gcaatccagg cattaggaaa
     5701 atctaaaatt actgctttga ttcattgtgc tgaattggtt ccttatttag cccttttgta
     5761 ttttatggtc gaaaaatatg ggttactggg tgccgcaata tcctggagca tacgtgtaat
     5821 tttagatgct ctgttattac aatggcttta tactagaatg tgttctgtat atgaaaacta
     5881 aaattaacca attactgagt cacttggata atgaatagta atatttacgc tgtcattgtg
     5941 acatataatc ccgaacttaa aaatctgaat gcactgatca ctgaactaaa ggagcaaaat
     6001 tgttacgttg tagtagtcga taatagtact aatttcacct tgaaagataa attagcagac
     6061 attgaaaaag ttcatttaat ttgtcttggt aggaatgaag gtatagcaaa agcacaaaat
     6121 attgggattc gatattctct tgaaaaaggg gcagagaaga taattttttt tgatcaagat
     6181 agccgtattc gcaatgagtt tatcaaaaaa ctttcctgct atatggataa tgaaaatgca
     6241 aaaatagctg gcccagtttt tattgataga gataagtcac attattatcc tatttgtaat
     6301 atcaaaaaaa atggtcttcg agagaaaatt catgtcactg aaggacagac accgtttaaa
     6361 agttcagtaa caatctcatc cggaaccatg gtttcaaaag aagtttttga gattgttgga
     6421 atgatggatg aggaactttt tattgattat gtcgatacag aatggtgtct tagatgctta
     6481 aactatggca tattagttca tatcattcct gatatagaaa tggttcatgc tattggggat
     6541 aagtcagtaa aaatctgtgg gattaacata ccaattcact cgccagtacg tcgttattat
     6601 cgagtaagaa atgcatttct tttgcttaga aaaaatcatg tgcctctttt actctctatt
     6661 agggaagttg ttttttcttt aattcatacg actttgatta tcgcaactca aaaaaataaa
     6721 attgaatata tgaaaaaaca tattttggct acgctggatg gaataagagg aattactggg
     6781 ggcggtcgtt ataatgcata gttctgatca aaaaagagta gctgtactta tggctacgta
     6841 taatggcgaa tgctggattg aggaacaatt aaaaagtatt attgaacaaa aagatgtaga
     6901 catctcaatt tttatatcag atgatttatc aactgataat acattaaata tttgtgaaga
     6961 atttcaagta tcatatccct caattataaa tatacttcca agcgtcaata agtttggggg
     7021 ggcaggtaaa aatttttata gattaattaa agatgtcgat cttgaaaact atgattatat
     7081 ctgttttagt gatcaggacg atatttggta taaagataaa ataaaaaatg caattgactg
     7141 cttggtattt aataatgcga attgttattc cagcaatgta attgcatatt atccatcagg
     7201 gcaaaaaaat ttagttgata aggctcagtc gcaaactcaa ttcgattatt tttttgaagc
     7261 agcaggtccg gggtgtacat atgttataaa aaaagaaacg cttattgaat ttaagaagtt
     7321 catcataaat aataaaaatg ctgcacaaga tatctgtctg catgattggt ttttatattc
     7381 atttgcgcgg acgagaaatt attcgtggta tattgataga aaaccaacaa tgctgtacag
     7441 acagcatgaa aacaaccaag tgggagcaaa cattagtttt aaagcaaaat acaaacggct
     7501 tggtttggtg agaaacaaat ggtatcgaaa agaagtaact aaaatagcta atgccttagc
     7561 tgatgatagc tttgtgaata atcaattggg taaaggttat ataggcaatt tgatcttggc
     7621 tttaagtttt tggaagttaa ggcggaaaaa ggctgataaa atttatattt tattaatgtt
     7681 gattttaaac atattttaaa aaaacatcac cttacgaata tgaataatat aaataaaatt
     7741 tttataacat ttttatgtat tgaactgatt attggtggtg gtggaagatt actggagcca
     7801 ttgggaatat tccctttgcg atatttatta tttgtattta gttttatact tttaattttt
     7861 aatttagtta cattcaattt ttcaatcacc caaaaatgtg tcagtctttt tatatggttg
     7921 cttttattcc ctttttatgg cttctttgtc ggcttattag ctggtaataa aataaatgac
     7981 atactgtttg atgtgcaacc ataccttttt atgctgtcac ttatatatct atttacacta
     8041 agatatactt taaaattatt tttatgtgag atttttatta aaatagttaa tgcatttgca
     8101 ttatatggat cactgttata tatttcatac ataattttgt tgaatttcgg tttgttaaat
     8161 tttaatttaa tttatgaaca cttatcattg actagcgagt tcttttttcg tcccgatggg
     8221 gctttttttt ccaaatcctt ctactttttt ggtgtcggtg cgattatcag ttttgtcgac
     8281 aaaaaatatt taaaatgtct cataatagtg cttgcgatat tattgacaga atcaagaggt
     8341 gtattacttt ttacaacatt atcactgtta ttagccagtt ttaaattaca taagctatat
     8401 ttaaatacta ttataataat attgggcagc gttctattta taattatgct ttacatggtc
     8461 ggatcacgca gtgaagattc tgactctgtt agatttaatg atttatattt ttattataaa
     8521 aatgttgatt tagcgacgtt cttgtttgga agaggatttg gttcatttat attagatcga
     8581 ttaaggattg aaatagtacc tcttgagata cttcagaaaa caggcgttat tggtgtattt
     8641 atatcattag ttcctatgtt gcttatcttt gtgaaaggct attttttaaa tagtacaaaa
     8701 acatcattaa tgatgtcgtt aatacttttt ttcagtatta ccgtttctat aactaatcca
     8761 tttcttttta cacccatggg aatttttatt ataggcgttg tagttttatg ggtattttct
     8821 atagaaaata tccaaattag taataacctc acttctggag caaaataaag gatgttaaaa
     8881 atagggaagt tattgaccag ttcttttttt agttattttt taattggaat tgtcaatact
     8941 gctttacatt ggggggtatt ttatgcgtgt tataataatt tagcttttgg tcaaggccga
     9001 agtaacattg ttggttttat atgtgcagca accttttctt tttttgcaaa tgcaagatac
     9061 tcatttaaag tatctgcaac caaggctaga tattttatat ttatattttt tatgggggcg
     9121 atgagttatt tattcggcgt tttatttgat ctcttagctt tatctccaat atttacattg
     9181 ttcacctttt ctctttttag tttggtttta ggatattgcg catcctgtct gtctttacac
     9241 ttaggaataa taaaatactt tgggtatttt tctcgattgt tttaacatat gtcttatttt
     9301 ggttttctgt tacgcaagtt gacaggtatt tatcacccat ttacccattg tcagtattgc
     9361 tggttgcgat ctcaattgca agtttaataa aagatatcaa cattacaaat tacaagttgg
     9421 gacgatattt tatccttgcg tgtattattt tttttcgggg cgatcgttgt attttgaatt
     9481 caaatatttt tattctgaat acttgaattt taattcacgc ttagaaagtc gctatagcta
     9541 tgaactgatg aagaaagcaa gtgaatatag tgagttatat ggagataatc ttatccaatt
     9601 aggcttggag gatggtattt acttctataa agggatggca attggtgacg tctttggatt
     9661 agcaagatat agtgattgga ctatttgtaa tcccgagtgt gaggtaatcc cacaggatga
     9721 tttaatagag aagatgaaat cttttaatag ttcgtttatc gttatatcta aacgaagcta
     9781 tgctaatttt aatccagaaa agtatccaaa attcaaagtt cttatggata cacccaatgg
     9841 catacttatc gctataaagt aagaagcatg ctgtaaccat atgtattcct tgattagaag
     9901 atgaaaatca cgacggttaa taatataacg gttaatagac ggttaataat atagaagaca
     9961 ggagtaaaca atgtcaaagc aacagatcgg cgtagtcggt atggcagtga tggggcgcaa
    10021 ccttgcgctc aacatcgaaa gccgtggtta taccgtctct attttcaacc gttcccgtga
    10081 aaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt actatacggt
    10141 gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg
    10201 tgcaggcacg gatgctgcta ttgattccct taagccatac ctcgataaag gtgacatcat
    10261 cattgatggt ggtaatacct tcttccagga caccattcgt cgtaaccgtg agctttctgc
    10321 cgaaggcttt aacttcatcg gtaccggtgt ttccggcgga gaagaggggg cgctgaaagg
    10381 gccgtccatc atgcctggtg gccagaaaga agcctatgaa ctggttgcgc cgatcctgac
    10441 caaaatcgcc gccgttgctg aagatggcga accgtgcgtt acctatatcg gtgctgatgg
    10501 cgcaggccat tatgtgaaga tggttcacaa cggtattgaa tacggtgata tgcaactgat
    10561 tgctgaagcc tattctctcc tgaaaggcgg cctgaatctc tctaacgaag aactggcaca
    10621 gacctttacc gagtggaata acggtgaact gagcagctac ctgatcgaca tcaccaaaga
    10681 catcttcact aaaaaagatg aagacggtaa ctacctggtt gatgtgatcc tggatgaagc
    10741 ggctaacaaa ggtaccggta aatggaccag ccagagcgcg ctggatctcg gagaaccgct
    10801 gtcgctgatt accgagtctg tgtttgcacg ttatatctct tctctgaaag atcagcgcgt
    10861 tgccgcatct aaagttctct ctggtccgca agctcagtca gcaggcgaca aggctgagtt
    10921 catcgaaaaa gttcgccgtg cgctgtatct gggcaaaatc gtttcttacg ctcagggctt
    10981 ctctcagctg cgcgcagcgt ctgaagagta caactgggat ctgaactacg gcgaaatcgc
    11041 gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc
    11101 ctatgccgaa aatccgcaga tcgctaacct gctgctggct ccgtacttca agcagattgc
    11161 cgatgactac cagcaggcgc tgcgcgatgt cgtcgcttac gcagtacaga acggtattcc
    11221 ggttccgacc ttcgccgctg cggttgccta ttatgacagc taccgttctg ctgttctgcc
    11281 tgcgaactta atccaggccc agcgcgacta gggcaattgt ggccgcaggg atcaaagaaa
    11341 cctcataaat aaaagttata caat
//
