LOCUS       AB812059               12191 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 1111-55.
ACCESSION   AB812059
VERSION     AB812059.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1111-55"
                     /db_xref="taxon:562"
                     /note="serogroup:O136"
     gene            <1..793
                     /gene="galF"
     CDS             <1..793
                     /gene="galF"
                     /note="O136_01"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01694.1"
                     /translation="PMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLE
                     QRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVID
                     DASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVE
                     FIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVD
                     AMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             1164..2162
                     /note="O136_02"
                     /gene="psb1"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-N-acetylglucosamine
                     4,6-dehydratase"
                     /protein_id="BAQ01695.1"
                     /translation="MFDNKTILVTGGTGSFGNKFVRMTLENYNPKKIIIYSRDEMKQW
                     EMAKKFKDENRIRFFIGDVRDKDRLYRALDGVDFVVHAAATKIVPTAEYNPFECVKTN
                     INGAMNVIDACIDKGIERVVALSTDKASSPANLYGATKLASDKLFVAGNSYSGATKTR
                     FAVVRYGNVMGSRGSVIPFFLSIKENGELPITDERMTRFMITLEQGVELVWHAFKDMV
                     GGEVYVKKIPSMKVTDIATAVAPNAKQKIIGIRPGEKLHEQMISAEDSYYTYEYPEHF
                     KILPAIHNWCNSPERIKDGKKVPEGFVYESDSNAEWMSIEELRQWIDDNREKVGNI"
     CDS             2162..3316
                     /note="O136_03"
                     /gene="psb2"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ01696.1"
                     /translation="MKFIPYGRQDISDEDISAVVDVLKSEFLTQGPYVPKFEKTIANY
                     VNVKHAVAVNSATSALHIACLALGMKKGDWLWTSPNTFVASANCALYCGANVSFVDID
                     ARTYNMSVSALEAKLMSAKNNGTLPKVVVPVAFAGQSCEMEAIYKLSKEYGFSIIEDA
                     SHAIGGSYQGEKIGNSHFADITILSFHPVKIITTAEGGMALTNNDSLAEKMQLFRSHG
                     ITRDINHMTKVSEGDWYYQQIDLGLNYRMTEIQAALGMSQLKRIDGFVTRRHELAERY
                     KKALEGIPISLPFQAEGGYSAFHLYPITVKNSKLRKSLFDYLRNKNIGVNVHYIPVHT
                     QPYYEKLGHKVGDYPVAEDYYSRALSIPMYSALTNEDQDYVIQCIREFFK"
     CDS             3313..4023
                     /note="O136_04"
                     /gene="psb3"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01697.1"
                     /translation="MNVAIIPARGGSKRIPQKNIKMFCGKPMIAWSINAARKSGVFDR
                     IIVSTDDAEIAAVARKYGAEVPFTRPEELSNDFAATIPVIRHAVEWLVNNGCIADFVC
                     CIYATAPFIRSEDIIRGLTIIREQQADYAFTVTRYPYPIQRALKIGHENQISMFSPEM
                     FHVRSQDLEESWHDAGQFYWGTVSAWLQEKPIFSANSYSIVLPRERVQDIDTPEDWRV
                     AEWLYKTMEFKNESVSAG"
     CDS             4001..5539
                     /note="O136_05"
                     /gene="psb4"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01698.1"
                     /translation="MKVFLRVDSSLSIGSGHIIRCLNLAIALRNVGAECIFISKKHRG
                     NILFKIEQAEFLYQVIPTPEEYDIYVSEEKYWLNGSQNDDALQFSLLIKKQCENPDII
                     IVDHYSLDYEWELIIKRNFPEAKLIVIDDLCNRPHCCDLLIDQTYLRHEKEYTCLNIW
                     GGKILTGPKYALLDPVFSKLREQSINRKTQLEFPQRLMITMGGVDVNNITGKVLRYIE
                     NKNLKSIEKITVILGSACPHREEIEALVADSKYPINILTNVENMAELMLEHDFAIGAM
                     GGTTWERCVMALPAVNIAIANNQSTIATNFSKAGAIVLHSDNFTKNDFYNAFNRLITD
                     YHQQRDLVMSICDGQGLIRDIQEIIPCFSTDGINVTLRFATLDDINFVYQLQCEPQTR
                     KFARNPNIPSYDSHTEWMRRKLVEQNSFFYIIEHIGACGVLRLDPIEHELAQYEISIF
                     LTTASMGKGIAKAAIRRATMLHKDTVILATVLYENFASHRLFEQIGFNKISSCEYINR
                     GGNE"
     CDS             5532..6581
                     /note="O136_06"
                     /gene="psb6"
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylneuraminate synthase"
                     /protein_id="BAQ01699.1"
                     /translation="MSKYITIDGRKIGKEYSPYVIAELSANHNGDINRAFKIMEAAKL
                     AGADAIKLQTYRADTITIDCNSEDFQIHGGLWDGQTLFNLYKGAQMPWEWHKPLFEKA
                     KELGITIFSSPFDFTAVDLLEELGAPAYKIASFEAIDIPLIKYVARTGKPMIISTGMA
                     NEQEIQEAIDAAKDGGCKELVVLHCVSGYPAPAEDYNLVTILDMAERYGVITGLSDHT
                     IDNTTAIASVSLGANVIEKHVTLDRKGGGPDDSFSLEPSELRALCKDVKTAWRALGKI
                     SYNHKESEKGNVKFRRSLYVVKDVAKGEEITTQNVRSIRPGFGLAPKHLEHVLGKKFL
                     TDLPAGTALTFDIIE"
     gene            6637..7875
                     /gene="wzx"
     CDS             6637..7875
                     /gene="wzx"
                     /note="O136_07"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01700.1"
                     /translation="MECRTVIMLLLRGATLASKFLLVIFLAKFATYKNLADYTIIAVT
                     ISYLLFFLGFDFYTYSTREIIKKGFTKSGELLCNQLYLYISMYLILIPIVYGLNYFSV
                     LSVGVLFYFVVITEHFAQECMRIIIINNKPVKANFQFFLRSSFWIYIYIAYCYWKATY
                     SLDILLLFWLISNVCSIVYSVSEFKMINYKDDKIYRVDFKWIKKGVAIAIPLLVTTLM
                     LRGGYVTDRYILKYLSSTEVLAVYSFYSNMSNALIAFIDAAVIMIFYPKVISAYNELN
                     IEEYNKTLVLFKRNVVKVGCCSFFILSVAVPVICLFLNKSEFINSIIMFYILLTSAFI
                     YSFSLVYHYELYARHKDFILFKCTFFSFLLTVIGQYVFASLFGGIGMAISILLFSLML
                     LSTKYLSIINIKKNECFGNS"
     CDS             7924..8952
                     /note="O136_08"
                     /gene="wetM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01701.1"
                     /translation="MNLYIIQNDTHFKNFIELATEGDFFIDLARFTGQDINYELFYKK
                     GVSEINLGLHDANFYLFSSYQKIKFCFNFEMIVKKIISAYRFEKIITGNDGALQKIII
                     KQALKNNSKGRVEMWLDGLISLNKNRVINSAKVFMSYIADKIGLSSYVPSVIGTSSYV
                     DTLYVMDQSVIKEYNFLMIKPKVKKIEKRLFPRHKKLISLAHNHERRAQVCKVLYLTS
                     AWSFHGHNKCQNIQYHQILNLLSNFEGNKDIDFQIRIHPRDIITNYSEIPVGVISRVK
                     SFEEDILSASIIISARSTGLFEADMIGKKVIVYDEGFEGDFMNEYFDTLPRCVDLNEM
                     NSFISQQG"
     gene            8961..10193
                     /gene="wzy"
     CDS             8961..10193
                     /gene="wzy"
                     /note="O136_09"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01702.1"
                     /translation="MSSTQVFSLLLGFYLVSVNLLIIPLDYFNVERSILIIPYVFIFS
                     LSLFFFALATIGDFYKSPKQIFYVQVCFVFVIIIPSCLSFIQNTLLEVVPSMMRYMSY
                     LFTFVFVYYFSAKNWFTVKNLDVSIKFISIILFIFSILQIVKGEMIYMNGAYRLSSIY
                     GTTPAGFALITLVFSVYFYSQLHVSDSGKFKKIFPFAFFMINVSMMALTQSRQSIMTL
                     LLLICIFHFVRAKNIFKFFSLIFILLLIYVFYLLVVNTDFFPRLTQMLFNYSSDSSTH
                     TRISIITQSYEHLDVNDLIYGIGLGGFNHFYYKISGEIGVAAHNDFFLFFVEGGVIAL
                     FFYCLFLCGGVSFWGRAIKQQGDSYFTPLLVFCALYVFSFLNNPYYYPQVQVVASALM
                     GVYLSEYIKKQKVNYVLP"
     CDS             10180..10920
                     /note="O136_10"
                     /gene="wfeD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01703.1"
                     /translation="MFCHELENKMKSQEFFVETLKNSQLNKTRISFLNPYSYPLISRN
                     KKIIKGFDYWFADGLTLCIVTNLFREKSDIIKRGSFDFSSLAGICFDYLQTHSLSVAI
                     IGGSNDEIHQACEYFSIRYPSLKICFKHHGYIDIDKCDHIINELKKSEVQALIVGMGT
                     PKQEEFILKVEKHIISCKLFLTCGGFISQTASSGDYYHPLIKRLGLRWLQRAVLHKHV
                     RRRLLLNYPCFYFRYICSAILNILYAKK"
     gene            11045..>12191
                     /gene="gnd"
     CDS             11045..>12191
                     /gene="gnd"
                     /note="O136_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01704.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFLDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENP"
BASE COUNT     3756 a   1993 c   2528 g   3914 t
ORIGIN
        1 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
       61 ggtaactcac gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga
      121 atctcttctt gagcagcgcg tgaagcgtca actgcttgcg gaagtgcagt ccatctgtcc
      181 accgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttag gccactccat
      241 tttgtgtgca cgacccgcca ttggtgacaa cccatttgtc gtggtgctgc cagacgttgt
      301 gatcgacgat gccagcgccg acccgctgcg ctacaacctt gcagccatga ttgcgcgctt
      361 caacgaaacg ggccgtagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata
      421 ctcggtcatc cagaccaaag aaccactgga ccgtgaaggt aaagtcagcc gcattgttga
      481 atttatcgaa aaaccggatc agccgcagac gttggactca gacatcatgg ccgttggtcg
      541 ctatgtgctt tctgccgata tttggccgga acttgaacgc actcagccag gtgcatgggg
      601 gcgtattcag ctgactgatg ccatcgctga actggcgaaa aaacagtccg ttgatgccat
      661 gctgatgaca ggtgacagct acgactgcgg taaaaaaatg ggttatatgc aggcatttgt
      721 gaagtatgga ctacgcaatc tgaaagaagg ggcgaagttc cgtaaaggga ttgagaagct
      781 gttaagcgaa taatgaaaat ctgaccgtat gtaacggttg ataagaaaat tataacggca
      841 gtgaagattt gtggcgaaag taatttgttg cgaattttcc tgccgttgtt ttatataaac
      901 aatcaggata acaacgagtt agcaatagga ttttagtcaa agttttccag gattttcctt
      961 gtttccagag cggattggta agacaattag cgtttgaatt tttcgggttt agcgcgagtg
     1021 ggtaacgctc gctacatcgt aggcatgcat gcagtgctct ggtagctgta aagccagggg
     1081 cggtagcgtg cataaactta gggaagttgt tacgaattat aaagatcgca gcaatttcgt
     1141 attgtgtctt atctggtgaa aaaatgtttg ataataaaac gattcttgta accggcggta
     1201 ccggttcatt tggcaataag ttcgttcgca tgacattaga aaactataat cctaaaaaga
     1261 ttattattta ttctcgtgat gaaatgaaac agtgggaaat ggcaaaaaaa ttcaaggatg
     1321 aaaatcggat ccgcttcttt attggtgatg ttcgtgataa agatcgtctt tatcgcgcat
     1381 tggatggcgt tgattttgtt gttcatgctg cagcaacaaa aatcgtccct acagccgagt
     1441 ataatccctt tgaatgtgta aaaacgaata tcaatggtgc tatgaacgtg attgatgctt
     1501 gtattgacaa aggcatcgaa cgcgtagttg cgctttctac cgataaagca agtagccctg
     1561 cgaatctgta tggtgcaact aagctagcat ctgataaact ttttgttgca ggtaactcct
     1621 attctggcgc gacaaaaacc cgttttgctg ttgttcgcta tggaaatgtt atggggtctc
     1681 gtggctcagt aataccattc ttcttatcta ttaaggagaa cggtgaactg ccgataactg
     1741 atgagcgtat gacacgcttt atgattactc tggagcaggg agttgaattg gtttggcatg
     1801 cgtttaaaga tatggtcggt ggtgaagttt atgtaaaaaa aataccttct atgaaagtca
     1861 ctgatatagc tacagctgtt gcacctaatg ctaaacaaaa aataatcggc attcgacctg
     1921 gcgagaaact ccacgaacaa atgatcagcg ctgaagattc ttattacacc tatgaatatc
     1981 cagagcattt taaaattctt ccagctattc acaactggtg taactcacct gaaagaatta
     2041 aagatggcaa aaaagtacca gaaggtttcg tttatgaaag tgatagtaac gcagaatgga
     2101 tgagcattga agaacttcgt caatggatcg acgataatcg tgaaaaagta ggtaacatct
     2161 aatgaaattt attccttacg gacggcagga tatttctgac gaagatataa gtgctgtagt
     2221 ggatgtactt aagtccgagt tcttgactca aggaccatat gttcctaagt ttgagaaaac
     2281 aatagctaat tatgtgaatg ttaagcatgc tgttgcagta aatagtgcga cgtcagcact
     2341 ccatattgct tgtctggcac tggggatgaa aaaaggcgat tggctttgga catcaccaaa
     2401 tacttttgtt gcttctgcga actgtgctct ttattgtggc gctaatgtca gttttgttga
     2461 tatcgatgcg cgaacatata atatgagcgt tagtgcctta gaagcgaaac tcatgtctgc
     2521 gaaaaataat ggaactttac ctaaagttgt tgttcctgtc gcatttgcag gacagtcatg
     2581 tgaaatggag gcaatatata aactctcaaa agagtatgga ttttcaatta tcgaagatgc
     2641 ttcacatgca attggcggaa gttatcaggg agaaaaaata ggtaattcgc actttgcaga
     2701 tattactata ttgagctttc atcctgttaa aattatcaca actgcagaag gggggatggc
     2761 tctaaccaat aacgatagtt tggcggaaaa aatgcagcta ttccgtagcc atggaattac
     2821 ccgtgatatt aatcatatga caaaagtaag tgaaggcgat tggtattatc aacaaattga
     2881 tctcggtctg aattacagaa tgaccgaaat tcaggcagcg ttgggtatga gtcagttgaa
     2941 acgtattgat ggctttgtaa cacgccgtca tgaattggct gagcgttaca aaaaagctct
     3001 tgaaggtatt cctatttctc tgccattcca ggccgaaggc ggctacagtg cttttcacct
     3061 ttatcctata actgttaaaa attccaaact gcggaaatca ctatttgatt atctacggaa
     3121 taaaaatatc ggggtcaatg tccattatat tccagttcat actcagccgt attatgaaaa
     3181 gttagggcat aaagtcgggg attatcctgt tgctgaggac tactactccc gggccctcag
     3241 tataccgatg tattctgctt taacaaacga agatcaggat tatgtaatcc aatgcattcg
     3301 ggagtttttt aagtgaacgt ggcaatcatt ccagctcgcg gtggcagtaa acgtatcccg
     3361 caaaaaaata tcaaaatgtt ttgtgggaaa ccaatgattg cttggtcgat taatgccgct
     3421 cgaaagagcg gcgtgtttga tcgaattatt gtttcgacgg atgatgcaga aattgcggct
     3481 gtagcgagaa aatatggcgc tgaagttccg tttacgcgcc cagaagaact ttcaaatgat
     3541 tttgctgcaa caattcctgt catacgacac gctgttgaat ggcttgttaa taatgggtgt
     3601 atagcagatt ttgtgtgctg tatatatgca actgcaccat ttattcgttc tgaagatatt
     3661 attcgtgggt tgacaataat tagggaacaa caggcagact atgcttttac tgttactcgg
     3721 tacccttatc cgatccaacg cgcactcaaa atagggcatg aaaaccagat tagtatgttt
     3781 tctcctgaaa tgttccatgt tcgttctcag gatctcgaag aatcttggca tgatgccgga
     3841 cagttttact ggggaacagt atctgcatgg ttgcaagaaa agccaatatt tagcgctaac
     3901 tcctattcaa ttgttctacc gcgtgagcgt gtccaagata ttgatactcc agaagactgg
     3961 cgagtagctg agtggttata taaaaccatg gagtttaaaa atgaaagtgt ttctgcgggt
     4021 tgattcgtct ctatctatcg gctccggtca tattattaga tgtttaaatt tagcaatagc
     4081 actaaggaac gtaggggctg aatgtatttt tatatctaaa aaacatcgtg gcaatatttt
     4141 gtttaagatc gaacaggccg aatttttata ccaagtgatt ccgacacctg aagagtatga
     4201 tatctatgta agcgaagaaa aatattggct caatggtagt caaaatgatg atgctttgca
     4261 attcagttta ctgataaaaa aacaatgtga aaatccagat attattatcg ttgatcatta
     4321 ttcactcgat tatgaatggg aattaataat taagcgtaat ttccctgaag ctaaacttat
     4381 agttatcgat gatctttgta atcgccccca ctgttgtgat ctattgatag atcagacata
     4441 tttacgtcat gaaaaagaat atacatgctt aaacatttgg ggagggaaga tattaacggg
     4501 gcccaagtat gcactactag atcctgtttt ttcaaaatta agggagcagt caataaatcg
     4561 taaaactcag ctagaatttc ctcaaaggct aatgataaca atgggaggag ttgatgtaaa
     4621 taatataact gggaaagtct tgcgttacat tgaaaataaa aacctaaaaa gcatcgaaaa
     4681 aattactgtt atactcggta gcgcgtgtcc gcatcgggag gaaattgagg cattggttgc
     4741 tgattccaaa tatcctataa acatattaac caatgttgaa aacatggctg aacttatgct
     4801 agaacatgat tttgcgattg gtgcaatggg cgggacaaca tgggaacgtt gtgtaatggc
     4861 tttacctgca gtgaatatcg caattgccaa taaccagagc acgattgcca cgaatttttc
     4921 taaagctggc gctattgttt tacattcaga taattttact aagaatgatt tttataatgc
     4981 ttttaatcgg ttgataactg attatcatca acagcgtgat cttgtaatga gtatatgtga
     5041 tggtcaagga ctcatcagag atatacagga aattattcct tgcttttcta cagatggcat
     5101 aaatgtgacg cttagatttg caacgttgga tgacattaat tttgtttacc aacttcagtg
     5161 tgagccacaa actcgaaagt ttgcacgaaa cccaaatatt ccttcatatg atagtcacac
     5221 agaatggatg cgtcgtaaat tagttgaaca aaatagtttt ttctatatca ttgagcatat
     5281 aggagcgtgt ggagtattgc gtttagatcc aatagaacat gagttagcac aatacgaaat
     5341 ttcgatattt ttgacgacag ctagtatggg gaaaggaatt gcaaaagcgg ctatcagacg
     5401 tgcaactatg ttacataaag atacagtaat actggcaacg gtactgtacg aaaattttgc
     5461 ttctcatcga ctttttgaac aaattggttt caataagatc tcttcatgtg aatatattaa
     5521 tagggggggg aatgagtaaa tatataacga ttgatggtcg gaaaattggt aaagaatact
     5581 caccatatgt cattgctgaa ttgtccgcaa atcacaatgg tgatataaat cgtgctttca
     5641 aaattatgga ggctgctaaa ttagccggcg ctgatgcaat aaaattacag acatatcgtg
     5701 ctgatacaat tactatcgat tgtaactctg aagattttca aattcatggt ggcctatggg
     5761 atgggcaaac tttatttaat ttatataaag gtgctcaaat gccctgggaa tggcataaac
     5821 cattatttga aaaagcgaaa gaactaggta ttaccatttt cagtagtcca tttgacttta
     5881 ctgcagttga tctactagag gaattaggtg cacccgcata taagattgct tcatttgaag
     5941 caattgatat acctctgatc aaatatgtag ccagaacagg taaaccgatg attatctcta
     6001 caggtatggc taatgaacaa gaaattcagg aagcaattga tgccgcgaaa gatgggggat
     6061 gtaaagagct tgttgtgttg cattgtgtta gcggatatcc agcaccagcg gaagattata
     6121 atttagtaac aatacttgat atggctgaga gatacggtgt gattactggg ttatccgatc
     6181 acacaataga taataccacg gctattgcat ccgtctcact tggagctaat gtaattgaaa
     6241 aacatgttac actggacaga aaaggaggtg gacctgatga tagtttttct ctggaaccta
     6301 gtgagctaag agcactttgc aaagacgtta agacagcttg gcgagcatta ggaaaaatca
     6361 gttataacca taaggaaagt gaaaaaggga atgtcaaatt cagacgatca ctttatgttg
     6421 taaaagatgt tgcaaaaggt gaggaaataa caacgcaaaa tgtgcgtagt attcgccctg
     6481 gttttggttt agctccaaaa catttagagc atgtgttagg aaaaaaattc ttgacagatc
     6541 tacctgcagg cacagcttta actttcgata ttatcgaata atatagtacc ctcttaacaa
     6601 agagggtgaa tcaagctcat tatataaatc gatgatatgg aatgtagaac agtaataatg
     6661 ttgctcctca gaggggcaac actcgcaagt aagtttttac ttgttatttt tttagctaag
     6721 tttgccacgt acaaaaattt agccgactat acaataattg cagtcactat tagttatttg
     6781 ttattttttt tggggtttga tttttataca tactcaacta gggaaattat aaaaaaaggc
     6841 tttaccaaaa gtggtgaact tttatgtaac cagttgtatt tatacatatc aatgtatttg
     6901 atattaattc caatagtata cgggttaaat tatttttctg ttctaagtgt tggtgttttg
     6961 ttttattttg ttgttataac agaacatttc gcacaagaat gcatgagaat cattattata
     7021 aataataaac ctgtaaaagc aaattttcaa ttttttttac gctcttcatt ttggatatat
     7081 atatatatag catattgtta ttggaaagcc acttattcat tggatatttt gcttttattc
     7141 tggttgatat cgaatgtgtg ctctatagtt tattctgtca gtgaatttaa aatgattaat
     7201 tataaagatg ataaaatata tagagttgat ttcaaatgga tcaagaaggg ggtggcaatt
     7261 gcaataccat tactcgttac cacattaatg ctgagggggg gatatgttac ggacaggtat
     7321 attttaaaat atctctcatc tactgaagta ttagctgttt actcatttta tagtaacatg
     7381 tctaatgctt tgattgcatt cattgatgct gctgtaataa tgatatttta ccccaaagtc
     7441 atttctgctt ataatgaact aaatatagag gaatataata aaacattggt gttatttaag
     7501 cgcaatgttg taaaggtcgg ttgctgttcc ttttttattt tgagtgtagc tgtaccagta
     7561 atatgtttat ttttgaataa atcagaattt attaattcta ttattatgtt ttatatatta
     7621 ttaacttcag catttatata ttcatttagt ctagtatatc attatgaatt atatgccaga
     7681 cataaagatt ttatattatt taagtgtaca tttttttcat ttctgcttac tgtcataggg
     7741 caatatgttt ttgcttcttt atttggtggc ataggcatgg cgatttcaat tcttttattt
     7801 tcattaatgt tattatccac aaagtattta tctattatca atattaaaaa aaatgagtgt
     7861 tttggcaact catagtagct aaactagaat caacaagtaa atatcactgg tttggagtaa
     7921 taagtgaatc tttatattat tcaaaatgat acgcatttca aaaattttat tgaattggct
     7981 actgaaggcg atttttttat agatcttgca aggtttacag gtcaggatat caattacgaa
     8041 ttattttata aaaaaggagt gtcagaaatt aatttagggt tacatgatgc taatttttat
     8101 ttgtttagtt cttatcagaa aattaaattt tgtttcaatt ttgagatgat tgtaaaaaaa
     8161 ataatatctg cttaccgctt tgaaaaaatc ataacaggga atgatggtgc attacaaaaa
     8221 ataataataa aacaagcatt aaagaataat agcaaaggaa gagtagaaat gtggttggat
     8281 ggtttaattt cattgaataa aaacagagta attaattcag caaaagtatt tatgtcgtat
     8341 atcgctgaca agattggttt gtcttcttat gtaccttcag ttatagggac aagtagttac
     8401 gttgatacat tgtatgtaat ggatcaatct gtaataaaag agtataattt tctaatgatt
     8461 aaaccaaaag ttaaaaaaat tgaaaagaga ttgtttcctc ggcataaaaa attaatatct
     8521 ttggcacata atcatgagag aagagctcag gtctgtaaag tattgtattt aacaagtgca
     8581 tggtcatttc atggacataa taaatgtcaa aatattcagt atcaccagat tttaaatttg
     8641 ttatctaatt tcgaaggtaa taaagatatt gactttcaaa ttcgaatcca tccaagagac
     8701 attataacaa actattctga aattccggtg ggggttattt ctagagtaaa atcatttgag
     8761 gaagatattt tatcagctag cattattatt tctgcaagat ctacagggct tttcgaggct
     8821 gacatgatag ggaagaaagt gatagtatat gacgaaggtt ttgaagggga ttttatgaat
     8881 gaatattttg atacacttcc cagatgtgtc gatttgaatg aaatgaattc atttattagt
     8941 caacaaggtt aatttatata atgtcttcta cacaagtttt ttcattgcta ctggggtttt
     9001 atttagtatc agtaaatctt ttaattatcc cattagatta tttcaacgta gaacgctcaa
     9061 ttctgattat tccatatgta tttatatttt ctctgagttt gttttttttt gcgttagcaa
     9121 ctattggtga tttttataaa tcgccaaaac aaatttttta tgtgcaagtt tgttttgttt
     9181 ttgtgataat aattccatca tgtttgagtt ttatacaaaa tacattatta gaagtagtgc
     9241 ctagcatgat gaggtacatg tcatatttat tcacatttgt atttgtctac tatttctctg
     9301 ccaaaaattg gtttactgtt aaaaatttag atgtgtccat caaatttata tcgattatct
     9361 tgttcatttt ttcaatactt caaatagtaa aaggcgagat gatatatatg aacggggctt
     9421 atcgtttgtc aagtatttat gggacaacgc ctgctggttt tgcgttgatt actctcgtct
     9481 tttctgttta tttctatagt caattgcacg tttcggatag cggtaagttt aaaaaaatat
     9541 ttccatttgc tttttttatg attaatgtat ctatgatggc gttgacgcaa agccgacaat
     9601 caataatgac attgttattg cttatttgta tatttcattt tgttagggca aagaatatat
     9661 ttaagttttt ttcgcttata tttattttat tgttaatcta tgtattttat ttgttggttg
     9721 ttaatacgga cttttttcca cgattaactc aaatgctttt taattactca tcagattcat
     9781 caacacatac acgtatttct attattactc aatcgtatga acatcttgat gttaatgatt
     9841 taatttatgg gattggttta ggcggattta atcacttcta ctacaagatt agtggtgaaa
     9901 taggtgttgc agcccataat gattttttcc ttttttttgt tgaaggtggc gtaatagctt
     9961 tgttctttta ttgccttttt ttatgcggtg gagtgtcatt ttgggggcgg gcgattaaac
    10021 agcaaggtga tagttatttt acaccgttgc ttgttttttg tgctttatac gtattttctt
    10081 ttttgaataa tccttattat tacccacaag tgcaagttgt tgcttctgca ttgatgggcg
    10141 tgtatttgtc agaatatata aagaaacaaa aggtaaatta tgttttgcca tgaacttgaa
    10201 aacaaaatga aatctcagga attttttgtc gaaactctca aaaatagcca gcttaataaa
    10261 accagaatta gttttcttaa cccctattca taccctttaa tttcaagaaa taaaaaaatc
    10321 atcaagggtt ttgattattg gtttgcagat ggtttaacac tatgtattgt tactaatttg
    10381 tttagagaga agtctgatat aataaaaaga ggcagttttg atttttcatc actggcggga
    10441 atatgtttcg attatttaca aactcattct ctttctgttg ctattattgg tggaagtaat
    10501 gatgagattc atcaagcatg tgaatatttt tccattcgat atccatcctt aaaaatttgt
    10561 tttaagcatc atgggtatat agatatagac aagtgtgatc atataataaa tgagcttaaa
    10621 aaatctgaag tgcaagcatt aattgtagga atgggaacac ctaagcaaga agagtttata
    10681 ttaaaggttg aaaaacatat aataagttgc aagctttttt taacttgtgg aggttttatt
    10741 tcacaaacag cgagtagcgg tgattattat cacccattaa tcaaaagatt aggtcttcgt
    10801 tggctacagc gtgcagtgct tcataagcat gttaggcgca ggcttttact caattatccc
    10861 tgtttttatt ttagatatat atgtagtgca atattaaata tcttatatgc taaaaaatag
    10921 caggataatt atcaatgtag tatgctaaaa ttgaccaata tcgtgaagtt atcaaatgtt
    10981 atatcagata acatgaaaca atatacgaac cgctacacgc agtaacccct gacaggagta
    11041 aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc
    11101 gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgaaaagac
    11161 cgaagaagtg attgccgaaa atccaggcaa gaaactggtt ccttactata cggtgaaaga
    11221 gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg
    11281 cacggatgct gctattgatt ctctcaagcc atatctcgat aaaggtgaca tcatcattga
    11341 tggtggtaat accttcttcc tggacactat tcgtcgtaat cgtgagcttt ctgcagaagg
    11401 ctttaacttc attggtaccg gtgtttccgg tggtgaagaa ggtgcgctga aaggtccttc
    11461 cattatgcct ggtggtcaga aagaagccta tgaactggtt gcaccgatcc tgaccaaaat
    11521 cgccgcagtg gctgaagatg gcgaaccgtg cgttacctat attggtgccg atggtgcagg
    11581 tcattacgtg aagatggttc acaacggtat tgaatacggc gatatgcagc tgattgctga
    11641 agcctattct ctgcttaaag gtggcctgaa tctttccaac gaagaactag cgcagacgtt
    11701 taccgagtgg aataacggtg aactgagcag ctacctgatc gacatcacca aagatatctt
    11761 caccaaaaaa gatgaagacg gtaactacct ggttgatgtg attctggatg aagcggctaa
    11821 caaaggtacc ggtaaatgga ccagccagag tgcgctggat ctcggcgaac cgctgtcgct
    11881 gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagatcagc gtgttgccgc
    11941 atctaaagtt ctctctggcc cgcaagcgca gccagcaggc gataaggctg agtttatcga
    12001 aaaagttcgc cgtgcgctgt atctgggcaa aatcgtttct tacgctcagg gcttctctca
    12061 gctgcgtgct gcgtctgaag aatacaactg ggatctgaac tacggcgaaa tcgcgaagat
    12121 tttccgtgct ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcatatgc
    12181 cgaaaatccg c
//
