LOCUS       GU068043                9205 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain RVC1787 serogroup O137 O antigen gene
            cluster, complete sequence.
ACCESSION   GU068043
VERSION     GU068043.1  GI:307340793
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RVC1787"
                     /db_xref="taxon:562"
                     /note="serogroup: O137"
     gene            69..440
                     /gene="wcnH"
     CDS             69..440
                     /gene="wcnH"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnH"
                     /protein_id="ADN43855.1"
                     /db_xref="GI:307340794"
                     /translation="MNKIIVDLDGTLTEEDTNDYTKVKPKLKVVKKLYEFKKMGFSIV
                     IMTARNMRTFDGNIGKINIHTLPVIIDWLNENNIPYDEVIVGKPWCGNKGFYVDDRAL
                     RPSEFARLNLQEINELFDKDK"
     misc_feature    69..9131
                     /note="O antigen gene cluster"
     gene            440..2029
                     /gene="wcnI"
     CDS             440..2029
                     /gene="wcnI"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnI"
                     /protein_id="ADN43856.1"
                     /db_xref="GI:307340795"
                     /translation="MLLILSSAYIDIDLQSEFGRLPPSMLPLGNKRLVQHQKKLLNTN
                     ELMIITLPKSYKISEWDRKWLDNNNIKIVFVNDGGALGESVLYAIEYIKTLNISEHLR
                     ILYGDTLILSNIKDDSNVIAVSNSEDNYNWARVENQNAYIKISTENDVDILGGDILCG
                     YFSFSDIDLLRECLISNNYNFIEAIKSYHAKSFLFVRKIDEWLDFGHVNTYYKSKMKY
                     TTQRAFNELYINSDWVCKSSQDYEKILAEANWFEKIPLELKKYIPQYLGKESKNNKSA
                     SYKLEYLCHTALNELYVFSELPLTVWKSILEKCLDFVRESKSYIPSDKERFSTINELL
                     IDKTNARLVSFCSEKQFSINDKWDFKGKRISVSDILDVAAKYIPNDNNPSLMHGDLCF
                     SNILFDFRAGRIKLIDPRGLSVDRNYTIYGSYYYDLAKLSHSILGMYDWIVAGYCDAN
                     ICERKIELSFVNDKKIENIKSIFIDMITAQFDITLNELYAMQIYLFLSMLPLHSDDVQ
                     RQKALFANVFRLYELLQEIEK"
     gene            2026..2781
                     /gene="wcnJ"
     CDS             2026..2781
                     /gene="wcnJ"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnJ"
                     /protein_id="ADN43857.1"
                     /db_xref="GI:307340796"
                     /translation="MIVIPMAGMSSRFRNAGYKKPKYMLKAHGITLFSHSVSSFKSYF
                     NNEVFIFIVKDIDGSEFVKTEVKKLGILHYHIILLEQTTRGQAESVYNGLKQLQEKIM
                     ISPTESLTIFNIDTIRCNFMYPKLHERGDGYLEVFSGEGDNWSFVKPVNNESTLVIET
                     AEKKPISKLCCTGLYYFSRLQYFFEAYDLYILTPKDSWDKGELYVAPLYNILIAQKKE
                     IHYHKINVGDVFFAGVPKEYEDFCKIDYESFVG"
     gene            2787..4013
                     /gene="wzy"
     CDS             2787..4013
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43858.1"
                     /db_xref="GI:307340797"
                     /translation="MNWKIKKNVELLILFLVLNQLVIDTFNGYFIFSSQGGGNISVIY
                     KIIIFFLFLFYELVSRDRFIVAFSIVTWGVFLTFFHLLSNNYTEVVMDLSEYIKLATT
                     FIVFLGVSKFSYINPLAFIKYLSVYSFCIILFNILLSAFGVGETSYSDFGYKGFFYAA
                     NALSGVFCIVACFLMYITLKKSLTYYIICAGVLALCSFAIGTKTGIVFVAISLVTIPL
                     LMEGKKAKIRVSLLSLVGFLCALVIYADKIYTSNLVLRIVHFYEVGGWSKMLFSDRDL
                     FLKYNIDQFLNSDPVSLLIGIGYAGINAFPKPLTEIDIADITIIYGMLTTSAYISFYI
                     GILFYSNGYLKKNCPRDIIAISRYICLVLMIVSSIAGHILFNGMVTLYLGIAMALPWW
                     MANYRTRHIKGLKINV"
     gene            4006..5388
                     /gene="wzx"
     CDS             4006..5388
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43859.1"
                     /db_xref="GI:307340798"
                     /translation="MFKSVGVGFLSRIISVLSNIIIIPIILKHLTPEQFSLWMLFVSF
                     YSLVIIFDFGFSATISRYVSYVLSGATLQQLQNGFYENVWLGNGGGIDYKALNELSRI
                     NKIIFYYLVLIASVFLLCIYLYYCNFVGINISKDDRIAWGLFSISILIQLASIRYNGF
                     LHGSGNVAKIYRNTICSNFVFLLFFIFFIYLQWSLVGICIARLLSSLMVFILNAISSS
                     KLTCVYPRLRCNEVKGHDNKRLFKRIRDKSFHLGIGGLGNFICNRMTIMVMTATVAIQ
                     NIAGVSFIINLSITILSISLILINNAMPELVRFRVEGAYDKLFNSFKRICFISIVFFI
                     SSYVVMVISTPYIIKITHSKVILPSNSIVLLCFCIFFIELIQSLSISFISTSNNTDFA
                     KYQIITGLSFLLICFVFSYFKWTSLVNILLVQLIVQCIYNGWKWPFVIFCEYNKVRHL
                     IRKKNDVAAK"
     gene            5369..6430
                     /gene="wcnK"
     CDS             5369..6430
                     /gene="wcnK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnK"
                     /protein_id="ADN43860.1"
                     /db_xref="GI:307340799"
                     /translation="MMLLLSRNSLPVLKEYRGLEIKCLKNYIVDMDFFAYKDSMHIYE
                     ESTVNEYISNKGWVSKRAKNHPDLGFCYNKASIDKNGSILYSKQKYVTRNNDCDFFLF
                     DYSWGHNYQHWLISSLGRLFVYCELKKYNPKLKLLIGDKMPKYKSEAITLLGISDDDL
                     ICHREVTKYRKIYTCNFNSGSGLRVSHLSYYYYEKMANLINFTDDFGRKIYIARDDSR
                     GKRPLINRSELNALMTKRGFNVVFLEELSFYEKIALFKSADIIIGDFSSGWGHIVFCK
                     KFTKLILLEHDIYKFQGFYKEIANSKCLDLKVVETKNIFRLMYLKFLKLIWTLLKDVD
                     KRANSLSWKVNIKELMDFL"
     gene            6427..7173
                     /gene="wcnL"
     CDS             6427..7173
                     /gene="wcnL"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnL"
                     /protein_id="ADN43861.1"
                     /db_xref="GI:307340800"
                     /translation="MIKIYTINLFTENARREKILSLAKKLELPIKIENAIIGSNLSKE
                     YLDNLTNESIDKIGRNLSPGEVGCYLSHIKCLKNFLSSGDEFAIILEDDVTLDYRIKD
                     FISTIEFKETTLFFDVMLLGYRNGYGSYWGKKKWNSHKLLRFPDCGYGAHAYLVTRKG
                     AEKIVYNNAIPIWPYDYVTGGRADKTIRVYGVEKKIVDLDFYNSSCSSLEAERNYLGA
                     YSSEYKVKTPFVLYVLKKLIKGLKPIRVYK"
     gene            7170..8225
                     /gene="wcnM"
     CDS             7170..8225
                     /gene="wcnM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnM"
                     /protein_id="ADN43862.1"
                     /db_xref="GI:307340801"
                     /translation="MKVLIVSNMYPSIENPSLGVFVKNTEEMLVNANIDVDRAVIDRI
                     YSNSFEKVFGYFIFYLKVIYKILFCKCDIVYAHYVSHVAIPIFFVNLFKHIVVFSHVH
                     GGDVKQLKGTSAVFFKIKQALSKKIMDISKVIFSPSASYKRHIIELYSQDESKIIIFP
                     SGGIDTTLFSFGSGRKKNILGYAGRLEDSKNVDLIIKSLIDNKYNLEIVGDGKKKGQL
                     QELVSKYNLSERVIFHSSKTQKELSEWYKRVSILIYPSSSESLGLVPLEALACGTDVL
                     LSPIDAFFEFRDIGLQFEFLSELTPQAINVGIEKIISTRCFRNNMSNNIIINSVYARN
                     VVCKEFIDVFKQKCHCF"
     gene            8194..8676
                     /gene="wcnN"
     CDS             8194..8676
                     /gene="wcnN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnN"
                     /protein_id="ADN43863.1"
                     /db_xref="GI:307340802"
                     /translation="MYSNKNAIVFSYGAGGHSAQANRLAALLIPRLDSFYIISISDDC
                     ITPKWSHIHFVTGEVRKKNSYFDTLTNMGPIKIIQTLLKIKKYNVRCVISNGPGISVI
                     TALFFKVFGAKIIHVETWSRFVSKSLTGMAMYYIADVFYVQNKELCKIYKNAIYSGRL
                     "
     gene            8673..9131
                     /gene="wcnO"
     CDS             8673..9131
                     /gene="wcnO"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnO"
                     /protein_id="ADN43864.1"
                     /db_xref="GI:307340803"
                     /translation="MKIFTTVGTTSFDLLIKSVDEFAATNKDNDFIFQIANGQFHPRN
                     GCYFRFENDIVHYYEWADVVITHAGAGTIYKLLEQRKKVIIVPNLVRIDKHQRDIAQY
                     MCDHHYSLVLWDLSSLSSIMKDIEKFSPAVYKKTPFFRSDEIVNFINNIR"
BASE COUNT     3053 a   1130 c   1700 g   3322 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tatttgctta tttttatcgt gtaatcaaag
       61 tgattgttat gaataagata atagttgatc ttgacggtac tttaactgag gaagatacaa
      121 atgattatac taaagttaaa ccaaaactaa aagttgttaa aaaattgtat gaatttaaaa
      181 aaatgggatt cagtattgta ataatgacag caaggaatat gcgaacattt gatggtaata
      241 ttggaaagat taatatacat acacttcctg taatcattga ctggcttaat gaaaataata
      301 tcccgtacga tgaagttatt gtggggaagc catggtgcgg taataaggga ttttatgttg
      361 atgatcgggc actaaggcct tctgaatttg caagattaaa tcttcaagag ataaacgaat
      421 tatttgataa ggataaataa tgcttttaat actatcgtct gcgtatattg atattgattt
      481 acagtctgaa tttggtcggt tgccaccgag catgttaccg ctaggaaata aacgattagt
      541 tcaacatcaa aaaaaacttc ttaacacgaa tgagttgatg ataatcactt tacctaaatc
      601 atataaaata tcagaatggg acaggaagtg gttagataat aataatataa aaatagtctt
      661 tgttaatgat ggtggggcgc ttggtgaatc ggtattatat gctattgaat atattaaaac
      721 attaaacatt tctgaacatc tcaggatttt gtatggagat actttgattc tttcaaatat
      781 aaaagatgac tcaaatgtaa tcgctgtatc taactctgag gataattata actgggctag
      841 agtcgaaaat cagaatgcat atataaaaat atccaccgaa aatgatgtgg acattttagg
      901 aggagatata ctttgtggct atttttcatt tagtgatata gatcttcttc gggagtgttt
      961 gatttctaat aattataact ttattgaagc cataaaaagt taccatgcaa aaagttttct
     1021 atttgttaga aaaattgatg aatggttgga ttttggtcat gtgaatactt attacaagtc
     1081 aaagatgaaa tatacaacgc aaagagcatt caatgagtta tatattaact cagattgggt
     1141 atgcaaatct agtcaggatt atgagaagat tttagctgag gcgaactggt ttgaaaaaat
     1201 accactggaa ttaaaaaaat atataccaca gtacttaggt aaagagtcta aaaataataa
     1261 gagtgcaagt tataaactag aatatttgtg tcatactgca ctaaatgaat tatatgtctt
     1321 tagtgaatta ccgctaactg tatggaaatc aatattggaa aaatgccttg acttcgttcg
     1381 agagagtaaa agttatatac catctgataa agaacgattc tcaacaatta atgagttatt
     1441 gattgacaag accaatgcta ggcttgtatc tttctgttca gaaaaacaat tttctattaa
     1501 tgataaatgg gactttaagg ggaaaaggat tagtgtctct gatatacttg atgtagcggc
     1561 taaatatatc cctaatgata ataatccatc tttgatgcac ggagacttat gctttagtaa
     1621 tatattattt gattttagag caggtagaat taaattaatt gatcctcgag gattatctgt
     1681 tgatagaaat tatacaattt atggttcata ctattatgat ttagctaaac ttagtcattc
     1741 aatattggga atgtatgatt ggatagtggc aggctattgt gatgcgaaca tctgtgaacg
     1801 aaaaatagaa ttgagttttg ttaatgataa aaaaatcgaa aatataaaaa gtatttttat
     1861 tgacatgatt actgcacaat tcgatattac tctaaatgaa ttgtacgcaa tgcaaattta
     1921 tctattctta tcaatgttac cactccattc agatgatgtg caacggcaaa aagcattgtt
     1981 cgcaaatgtc tttagattat atgaactttt acaggagatt gaaaaatgat cgttatacca
     2041 atggctggta tgagttctcg atttcggaat gcaggctata aaaagccgaa atatatgcta
     2101 aaagctcatg gtatcactct gttctctcat tctgtttcta gctttaaaag ttattttaat
     2161 aatgaggttt ttatctttat tgtaaaagat attgacggaa gtgaatttgt taagactgaa
     2221 gtgaaaaaac tcggaatttt gcattatcat attattttgt tagagcagac aactcgaggt
     2281 caagctgaat cagtatataa tggattgaaa caactccaag aaaaaataat gatcagtcca
     2341 accgagtctt taactatttt taatattgat actataagat gtaattttat gtatccaaaa
     2401 cttcatgaaa gaggtgatgg ttatcttgag gttttttcag gagaagggga taattggtca
     2461 tttgtgaaac ctgttaataa tgagtcaaca ttagtaatag aaacagctga aaaaaaacca
     2521 atatcaaaat tatgctgtac tgggttgtat tatttctcta gactccagta ttttttcgaa
     2581 gcttatgatt tatatatttt aacacccaaa gatagttggg ataaagggga gctttatgta
     2641 gctccgttat ataatattct gatcgcccaa aagaaagaaa tacactacca caaaataaat
     2701 gtaggtgatg tgttttttgc tggtgttccc aaggaatatg aagatttctg taagatagat
     2761 tatgaatctt ttgttgggta ggttagatga attggaagat taagaaaaac gttgagctgt
     2821 taattttatt cttagtgctt aaccagttgg ttattgatac tttcaatggt tactttattt
     2881 ttagttctca aggggggggg aatatatcag ttatttataa aataattata ttttttctct
     2941 ttttgtttta tgagttagtg agtagggata ggtttattgt tgcattttct atcgttactt
     3001 ggggagtttt cttaactttt tttcatttat taagcaacaa ttatacagaa gttgtaatgg
     3061 acttgtcaga atatataaag ctggcaacaa catttattgt ttttttaggg gtttctaaat
     3121 tttcatatat aaatccactg gcttttataa agtatttgag tgtttattcg ttttgtataa
     3181 ttttatttaa tattttattg agtgcatttg gtgttggtga aacttcatat agtgattttg
     3241 gctataaagg ttttttctac gcagctaatg ctctttctgg tgttttttgc atagttgctt
     3301 gttttttaat gtatataacg ctgaaaaaaa gtttgacata ttatataata tgtgcaggcg
     3361 ttttagcgtt atgctcgttt gctataggaa cgaagacagg catcgtattt gtggctatat
     3421 cattagtgac tattccttta ctgatggagg gtaaaaaagc aaaaatacga gtatctttat
     3481 tatcattagt tggtttttta tgtgccttgg ttatctatgc tgataaaatt tatacatcta
     3541 atttagtttt aagaattgtt catttctatg aagtcggtgg ttggagtaag atgctttttt
     3601 cggatcggga cctttttctt aagtataata ttgatcaatt cttaaacagt gatccagtgt
     3661 cattacttat agggataggc tatgctggta ttaatgcatt cccaaagcct cttacagaga
     3721 tcgatattgc tgatataaca ataatttatg ggatgttaac tacctctgct tacatttctt
     3781 tttatattgg cattttgttc tatagcaatg gatatcttaa aaaaaattgc ccaagagata
     3841 taatagcaat atctcgttat atatgcttgg ttttgatgat tgtgtcgtcc attgctgggc
     3901 atattctttt taatggaatg gttactcttt atttaggtat tgcaatggcg ttaccttggt
     3961 ggatggctaa ctatcgaact cgacatataa aagggttgaa aataaatgtt taagtcggta
     4021 ggtgttggtt ttttatctag aattatatct gtattatcta atattataat cattcctatt
     4081 atattaaagc atttaacgcc agagcaattt tcgttatgga tgttatttgt ttcattttat
     4141 agtttggtta taatatttga ttttggattt agtgctacaa ttagtagata tgttagttat
     4201 gttttaagtg gtgcgacctt acagcaatta caaaatggtt tttatgaaaa tgtttggcta
     4261 gggaatgggg gcggtattga ttataaagcc ctgaatgaat taagtagaat aaataaaatt
     4321 attttttatt atcttgtctt aattgcttca gtttttctac tttgtatata tttatattat
     4381 tgcaactttg tcggaatcaa tatttctaag gatgatagaa ttgcatgggg acttttttct
     4441 atatcgatat tgattcagtt ggcgagtatt agatataacg gattcttaca cggttcaggt
     4501 aatgttgcaa aaatttatag aaatacaata tgctcgaatt ttgtatttct gttgtttttc
     4561 atttttttta tttacttgca gtggtcatta gttggtattt gtattgctag acttctttca
     4621 tcgttgatgg tgtttatttt aaatgccata tcttcatcga aactgacttg cgtgtatccg
     4681 agactaagat gcaatgaagt taagggccat gataataaac gtttgtttaa acggattcga
     4741 gataagtctt ttcatcttgg tataggtggt ttgggaaact ttatatgtaa tcgtatgaca
     4801 attatggtta tgacggcaac agtcgcaatt caaaatattg caggtgtttc cttcatcatc
     4861 aatttgagta ttactattct ttcaatttca ttgatactga ttaataatgc aatgcctgaa
     4921 ctggttcgtt ttcgtgtcga gggggcgtat gataaactct ttaattcctt caaacgaata
     4981 tgtttcataa gcattgtttt ttttatatcc tcatatgtcg taatggttat aagcacaccg
     5041 tatattatta aaatcacaca ttctaaagta attcttcctt ctaactcgat tgtactgttg
     5101 tgtttctgca ttttttttat agagttgatc cagagtttat ccatatcatt tatttcgaca
     5161 agtaataaca ctgattttgc taaataccaa attataacag gactttcatt tttgttaata
     5221 tgcttcgttt tcagttattt taaatggact tcattagtta atattttact tgtacaatta
     5281 atagttcagt gtatatacaa tggctggaaa tggccatttg tgatcttttg tgaatataat
     5341 aaagttagac accttattag gaaaaaaaat gatgttgctg ctaagtagaa attcgctgcc
     5401 tgtacttaaa gaatatcgag gactagagat taaatgtctc aagaactata ttgttgatat
     5461 ggattttttt gcctataaag atagcatgca tatttacgaa gagtcgacag taaatgagta
     5521 tatctccaat aaggggtggg tctcaaaaag agctaaaaat cacccagatt tggggttttg
     5581 ttataataag gcaagtattg acaaaaatgg tagtatttta tattcaaaac aaaaatatgt
     5641 aacaagaaat aatgattgtg attttttctt atttgattat tcgtgggggc ataattacca
     5701 acattggtta atatcttcat tggggcgctt gtttgtttac tgtgagctga aaaaatacaa
     5761 cccaaagttg aagttattaa ttggtgataa aatgcctaaa tataagagtg aagctatcac
     5821 tttattaggt attagtgatg atgatttaat atgtcatcgg gaggttacaa aatatagaaa
     5881 aatatacacg tgtaatttca atagtggctc tgggcttaga gtcagtcatt tatcatacta
     5941 ttactatgaa aaaatggcta atctaattaa ttttaccgat gattttggac gtaaaatata
     6001 tatagcgagg gatgactctc gtggtaaaag acctttaata aatagaagtg agttaaatgc
     6061 attaatgact aaacggggat ttaatgttgt ctttttggaa gaattgagct tttatgaaaa
     6121 aattgctctt tttaaaagtg cagatattat tattggcgac tttagttctg gttgggggca
     6181 tattgtattt tgtaagaaat ttacaaagtt aattcttctt gaacatgata tatataaatt
     6241 tcaaggtttt tataaagaaa tagctaactc aaaatgttta gatcttaagg ttgtagaaac
     6301 aaaaaatatt tttcggctca tgtatttgaa gtttttaaaa ttaatttgga ccttgttaaa
     6361 ggatgttgac aagcgtgcta actctctgtc atggaaagta aatattaaag aactaatgga
     6421 ttttctatga ttaagattta tactattaac ttatttactg aaaatgctcg cagagagaaa
     6481 atattgtctt tggcaaaaaa attggaacta ccgataaaaa tagaaaatgc aataataggt
     6541 tcgaatctat ctaaagaata tttggataat cttaccaatg aatccataga taaaataggt
     6601 agaaatttaa gtccaggaga agttggttgc tatttgtcac atattaaatg cttgaaaaat
     6661 tttctatcat caggagatga gtttgctata attttagaag atgatgttac tttagattat
     6721 cgaataaaag attttatcag taccatagaa tttaaagaga caacactttt ttttgatgtt
     6781 atgttgcttg gttatcgtaa tggatatggt tcttattggg ggaaaaaaaa atggaattct
     6841 cataaattac ttcgctttcc tgattgtggt tatggagctc atgcctactt ggttaccagg
     6901 aaaggtgcag aaaaaatagt atataataac gcaattccta tttggccgta tgattatgtc
     6961 actggaggta gggcggataa aacaattcga gtttatggtg ttgagaaaaa aattgttgat
     7021 ttagattttt acaatagctc ttgttcatcg ttagaggcag agagaaatta tcttggtgca
     7081 tattcttctg aatataaagt gaaaacacct tttgttctat atgtattaaa aaaattgatc
     7141 aaaggattaa aaccaataag ggtatacaaa tgaaggttct tatagtttct aatatgtatc
     7201 caagtattga aaatccttct ctgggtgtct ttgtaaagaa taccgaagaa atgttagtca
     7261 atgccaatat agacgttgat agagctgtca tagacagaat atattcaaat tcatttgaga
     7321 aagtatttgg ctattttatt ttttatttaa aagtcatcta taaaatttta ttttgtaaat
     7381 gtgatattgt ttatgcacat tatgtatcgc atgtagcaat accaattttt tttgttaatt
     7441 tatttaaaca catagttgtt ttctcgcatg tgcatggggg agatgtaaaa cagctgaaag
     7501 gaacgtcagc tgtattcttt aaaataaaac aagcattatc aaaaaagatt atggatatat
     7561 caaaggtgat tttttcccca tcagcttcat acaaaaggca tataattgaa ctttattcgc
     7621 aagacgagag caagattatt atttttcctt ccggagggat tgatacgaca ctattttctt
     7681 ttggttcagg gagaaagaaa aatatattgg ggtatgcagg tcgattagag gacagtaaga
     7741 atgttgattt aattataaaa tctcttattg ataataaata caatcttgag attgtaggag
     7801 atgggaaaaa aaaaggtcaa ttacaagaat tggtctctaa atataactta tctgaaagag
     7861 ttatatttca tagcagcaaa actcaaaaag agttgtcaga atggtataaa cgagtgtcta
     7921 ttttgattta cccgtcttca agtgaaagtt taggtttggt tcctttagaa gctcttgcat
     7981 gtggtacgga tgttctttta tcgccaattg atgcattttt tgaatttaga gatattgggt
     8041 tgcaatttga gtttttaagt gagttaaccc ctcaagcaat taatgtaggg attgaaaaaa
     8101 tcatttccac tagatgtttt cgcaataata tgagtaataa cattattatt aactctgtat
     8161 atgctagaaa tgttgtttgc aaggaattta ttgatgtatt caaacaaaaa tgccattgtt
     8221 tttagttatg gtgcaggcgg gcactcggca caagcaaaca gactggctgc gcttcttata
     8281 cctaggttag atagttttta tataatatca atatccgatg attgtattac acctaagtgg
     8341 tctcatatac attttgttac cggagaggtt agaaaaaaaa atagttattt cgatactctt
     8401 acaaatatgg ggccaataaa aataattcaa acattgttaa aaataaaaaa atataatgtg
     8461 agatgtgtta tttcaaatgg tccaggtata tctgtaatca ctgctttatt ctttaaagta
     8521 tttggggcga aaataattca tgttgaaaca tggtctcggt tcgtgtcaaa atccttaaca
     8581 ggaatggcta tgtattatat tgccgacgtg ttctatgttc aaaacaaaga attatgtaaa
     8641 atatataaaa atgcaattta ttcaggtcga ctatgaaaat atttactaca gttggaacaa
     8701 caagttttga tcttttaatt aagtcagttg atgaattcgc cgctacaaat aaagataatg
     8761 attttatctt ccagatagcg aatggacaat ttcaccctag aaatggttgt tactttcgat
     8821 ttgaaaatga tattgtgcat tattatgagt gggcagatgt tgtcataact catgcaggcg
     8881 caggcactat atataaactc ctggagcaaa gaaaaaaagt aatcattgtt ccaaatcttg
     8941 tgcgtattga taaacatcaa cgcgatattg cacaatatat gtgtgaccat cattattcct
     9001 tggtattgtg ggacctatca tctttatctt cgataatgaa agatattgaa aaattttctc
     9061 cagcggtata taagaaaact ccttttttta gaagtgatga gatagttaat tttattaaca
     9121 atataagata atttatacaa attgtatttt tctgacagga gtaaacaatg ccaagcaaca
     9181 gatcggcgtc gtcggtatgg cagtg
//
