LOCUS       DQ109551               14139 bp    DNA     linear   BCT 06-DEC-2005
DEFINITION  Escherichia coli O138 O-antigen gene cluster, complete sequence.
ACCESSION   DQ109551
VERSION     DQ109551.1  GI:73809576
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Wang,L., Liu,B., Kong,Q., Steinruck,H., Krause,G., Beutin,L. and
            Feng,L.
  TITLE     Molecular markers for detection of pathogenic Escherichia coli
            strains belonging to serogroups O 138 and O 139
  JOURNAL   Vet. Microbiol. 111 (3-4), 181-190 (2005)
   PUBMED   16280204
REFERENCE   2  (bases 1 to 14139)
  AUTHORS   Wang,L., Liu,B., Kong,Q., Steinrck,H., Krause,G., Beutin,L. and
            Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O138"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AAZ85703.1"
                     /db_xref="GI:73809577"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1137..12680
                     /note="O-antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AAZ85704.1"
                     /db_xref="GI:73809578"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4057
                     /gene="rmlA"
     CDS             3179..4057
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AAZ85705.1"
                     /db_xref="GI:73809579"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEKVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4062..4598
                     /gene="rmlC"
     CDS             4062..4598
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAZ85706.1"
                     /db_xref="GI:73809580"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETADFLYKTTNYYHPESESGLLWNDYTIDIAWPIE
                     NEPLLSAKDLKARRFLEI"
     gene            4632..5909
                     /gene="wzx"
     CDS             4632..5909
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAZ85707.1"
                     /db_xref="GI:73809581"
                     /translation="MSLIRNSSWNMVGYAIPSVVAIPALGYLARMLGTQQFGLFTLAL
                     AIVGYASIFDAGITRAVIREISFFRDNSIEKNKVIATSIICIFCLGGGGGLIFFLISP
                     FAVELLNITTNNYTMIQNSIIIVGLCIPVFLVNQLLMGIMEGEERFGAVNVQKVIANI
                     FIAAMPAVFVYFNQNIIYAVIGLLFGRVIGMIMAIYIFRLYIHVDKIHFYKNVFYRLL
                     KFGSWITISNIISPVMTISDRFILAHLMGANHIAFYTAPSEGIARLTIIPAALSRAIF
                     PKLSYAKSKKERKMYQRLGYLVLMLTCLPVVLFVFTFSSDILRIWMGPEFAADEPTLI
                     LRILVVGFLFNALAQIPFSSIQAEGKSHYTAAIHLCELVPYIYLLFFMINKYGIIGVA
                     YAWLARTLVDFVLLMITKTILDTRLKDLSINGG"
     gene            5899..6855
                     /gene="wfaF"
     CDS             5899..6855
                     /gene="wfaF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85708.1"
                     /db_xref="GI:73809582"
                     /translation="MEDKMAIGIVIVNWNAGEQLKNCLCSIIQYHSDVVDKVIIIDNN
                     SSDNSIENILMLETEAFDIIIIRENENLGFGKACNIASQKVTSKYILFLNPDAQIYSG
                     TLKQVVGCMEACDDIGICGIKLIDSFGNIQRHCTVQPKLKHYFNHATGLSTISSRLFP
                     YIFLKNFNYDSDGDVPHTIGAFYCIRNNLFKELNGFDELFFVYLEDLDLSRRVKQKGY
                     RIRYLASASAYHRGGGTSEKVKSLRLFYSLRSRILFGFKHFSLLQAYILVFLTLCVEF
                     ITRSIRCLIHFSLSDFHNTCEAYKILIKELPKILKKGLEIND"
     gene            6848..7924
                     /gene="wfaG"
     CDS             6848..7924
                     /gene="wfaG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85709.1"
                     /db_xref="GI:73809583"
                     /translation="MIDLLIFSKYGNMGASSRLRSLDYIECLEQENIRVTVSTLFDNE
                     YLENLYRKKKVSFISIFKYYIKRLVIVLFRVKKYDVVFIEKELFPYLPGIFEKLINIS
                     RVPYIVDYDDAIFHNYDMQQSWFKKMMLSGKLKPLLSRAAYVTVGNRYLEELSRENGA
                     KNVKIIPTVININRYHNQPTILFDEIKIGWIGSPSTTKYLKIIWPVLEKLSSKKHIKL
                     ITIGADKINDCPIPLEQYPWTLDTECELLNDISFGVMPLEDGLWERGKCGYKLIQYMA
                     SCKPVIASNVGVNTEIVTPKVGFLASDETEWLEAFNKFIDNPELISLMGVNARRRVEE
                     HYTLQINSNILIGLLKDVVEGRQI"
     gene            7985..9262
                     /gene="gna"
     CDS             7985..9262
                     /gene="gna"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-galactosamine dehydrogenase"
                     /protein_id="AAZ85710.1"
                     /db_xref="GI:73809584"
                     /translation="MIIAVVGLGYVGLPLAVEFGKSNQTIGFDLSESKINSYKEHIDP
                     TGEVSTEDLRKADKLVCTTDANSLKNADFIIIAVPTPVDESHQPDFTPLIKSSETVGK
                     NLKRGAIVVYESTVYPGATEEICIPILERFSGLKWKEDFNVGYSPERINPGDKERTVT
                     KIVKVVSGDTPVTLKKVRDTYEKIITAGVYCANSIKVAEAAKVIENTQRDLNIALMNE
                     LAIIFDKLSIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKADMIGYHPQV
                     ILAGRRINDSMGKYIAEQTVKKMIAANISIKGSKILVMGLTFKENCPDLRNSKVIDVI
                     KELESYGVNIIVHDPLVSSIDAYNEYGVILHEWDQIEPCDAVILCVCHNDYINAGFDA
                     IKSKLIPGGVFSDIKSIWTTEEVEAHGINSWRL"
     gene            9264..10289
                     /gene="gne"
     CDS             9264..10289
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-4-epimerase"
                     /protein_id="AAZ85711.1"
                     /db_xref="GI:73809585"
                     /translation="MNSYEIIKKNLLTSKKKWLITGVAGFIGSNLLEQLLKLDQDVVG
                     LDNFSTGHQHNLDLVKELVTPVQWSRFKFIKGDIRNLEDCRAACCEAQYILHQAALGS
                     VPRSIKDPIGTNDNNITGFLNMLVAANEQKVKRFVYAASSSTYGTHPGLPKVEDTIGD
                     PLSPYAVTKYVNELYAGVFSRTYGLESVGLRYFNVFGPRQDPNGAYAAVIPKWVASIL
                     NNEVIYINGDGSTSRDFCYIDNAVQANLLAATTLQPDAVNTIYNVAVGQQTSLNELYK
                     YIVDELVRYGVEINSNNLKYRDFREGDVKHSLANISKIKNKLGYEPLFTLKDGLVPSI
                     QWYYSNH"
     gene            10333..11484
                     /gene="wfaH"
     CDS             10333..11484
                     /gene="wfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85712.1"
                     /db_xref="GI:73809586"
                     /translation="MKKILFIVNVDKFFISHRLPIALECIKNGYEVHLTCAMTDSISY
                     LQKLGIITHPIPLSRAGISILNEINTCFHIYNTVKKIKPDIVHMVTVKPVAYGGLVCK
                     LLKIKKRVASISGLGYAFIDQSIKAKIIKNIVVLLYRISLKNKNTSVIFQNLNDKDYF
                     INCGIIKKHQSVLIRGSGVDLVRYKVKPEPLGKPVVMLVSRLLYDKGIREFIDAVRLV
                     KQKIDFSAVLVGSIDANPNSAKENEIMQWQNEGIIEYWGYRDDIPEVLCQSNLVVLPS
                     YREGLPKCLIEAAACGRAVVTTDVPGCRDAIIPNVTGILVEVKDHITLANAIETLIIN
                     PELRHQMAVKGRELAEQVFDIDNVVKLHLEIYKFIIPSCQRKINLCTSE"
     gene            11469..12680
                     /gene="wzy"
     CDS             11469..12680
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAZ85713.1"
                     /db_xref="GI:73809587"
                     /translation="MYLRMNNVLYTLSGLLLFLSILLPTTLSIVKIVLLLLLLFSCMF
                     ACKNAYSLSRQMVLLILFYSAIGILWVLYGAIRGNPGYLSMSTVLIFYPIIFGILSGL
                     YKERLNEELYKLFIYSAWFLSVITLLIIIDFIWLHFGVFDIATGLYDLDSVVVDNSSG
                     YFKFTIPNISSLIFLIPFLISAIFFRKPPKAHYILLLLLCVLAILSGRRALMLTMFLA
                     PVLAVVITSDRLSNNKIFCRFLLVFVLIGMAIYLFYIAYPDYFIDRVTNILNFDSNTS
                     NLERKYQFDALMAGFYEHPLFGNGAGAAASYLRSDSQPWAYELSYIAFLFQFGLVGFF
                     IYLMGILFIIYTLIRRVKFVGRNSMEFCFLSGFISFMIANATNPYLLKFDYMWLIFIP
                     LGIANSILVKK"
BASE COUNT     4224 a   2400 c   3076 g   4439 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcccc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 cagctgttgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgttacaacc ttgctgccat gattgcacgt ttcaatgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agagccgctg
      421 gaccgtgaag gtaaagtcag tcgcatcgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgatgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcgttc gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaaacg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatatgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg
     1321 ctgctgcaat ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacttggctg
     1381 ctgaaagcca tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagcggctc gcaattactg gtctgctctt gatggcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc
     1561 atcctgacga agtaaataat aaagaacaat tacccctctt tactgagacg acagcttacg
     1621 cgccaagcag tccttattcc gcatcaaaag catccagcga tcatttagtt cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc
     1741 actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg taaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc
     1981 cgaaagagaa atcttaccgc gagcaaatta cttatgttac cgatcgtccg ggacacgatc
     2041 gccgttatgc gattgatgct gagaagattg gtcgcgaatt gggttggaaa ccacaggaaa
     2101 cgtttgagag cgggattcgg aagacagtgg aatggtacct gtccaataca aaatgggttg
     2161 ataatgtgaa aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt
     2221 aatgaatatc ctcctttttg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct
     2281 ggcacctttg ggtaatttga ttgctcttga tgttcactcc actgattact gtggtgattt
     2341 tagtaatcct gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa
     2401 cgcagccgct cacaccgcag tagacaaagc agaatcagaa ccggagttcg cacaattact
     2461 taacgcgacg agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg cctgggttat
     2521 tcactactct actgactacg tatttccggg gaccggtgaa ataccatggc tggagacgga
     2581 tgcaaccgca ccactaaatg tttacggtga aaccaagtta gctggagaaa aagcattaca
     2641 agagcattgt gcgaagcacc taattttccg gaccagctgg gtctatgcag gaaaaggaaa
     2701 taacttcgcc aaaacgatgt tacgtctggc aaaagagcgt gaagaattag cggttattaa
     2761 cgatcagttt ggtgcgccaa caggtgctga actgctggct gattgtacag cacatgccat
     2821 tcgtgtcgca ctgaataaac cggatgtcgc aggcttgtac catctggtag ctagtggtac
     2881 cacaacctgg tacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggcattcc
     2941 ccttgcactc aacaaactca acgcagtacc aacaactgcc tatcctacac cagctcgtcg
     3001 tccacataac tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc
     3061 tgactggcag gttggcgtga aacgaatgct taatgaatta tttacgacta cagcaattta
     3121 atagtttttg catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac
     3241 tatggctgtc agtaaacagt tattacctat ttatgataaa ccgatgatct attacccgct
     3301 ctctacactg atgttagcgg gtattcgtga tattctgatt attagtacgc cacaggatac
     3361 tcctcgtttt caacaactgt tgggtgatgg tagccagtgg gggctaaatc ttcagtacaa
     3421 agtgcaaccg actccagatg gtcttgcgca ggcatttatc atcggtgaag agtttatcgg
     3481 tggtgatgat tgtgctttgg ttctaggtga taacatcttt tacggtcacg atctgccgaa
     3541 gttaatggac gcagctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa
     3601 tgatcctgaa cgctacggtg tcgttgagtt tgataaaaac ggtacggcaa ttagtctgga
     3661 agaaaaaccg ctacagccaa aaagtaatta tgcggtaacc gggctttatt tttatgataa
     3721 cgacgttgtc gaaatggcga aaaatcttaa gccttctgcc cgcggtgaac tggaaattac
     3781 cgatattaac cgtatctata tggaacaagg gcgtttatct gttgccatga tggggcgtgg
     3841 ttatgcgtgg ttagacacgg ggacacatca gagcctgatt gaggcaagca actttattgc
     3901 aacaattgaa gagcgtcagg ggttgaaagt ttcctgcccg gaagaaattg cttaccgtaa
     3961 agggtttatt gatgctgaga aggtgaaagt attagctgaa ccgctgaaaa aaaatgctta
     4021 tggtcagtat ctgctgaaaa tgattaaagg ttattaataa aatgaatgta attaaaacag
     4081 aaattccaga tgtactgatt tttgaaccga aagtttttgg tgatgagcgt ggtttcttta
     4141 tggaaagctt taatcagaaa gttttcgaag aagctgtagg acgtaaggtt gaatttgttc
     4201 aggataacca ttcgaagtct agtaaaggtg ttttacgcgg gctgcattat cagttagaac
     4261 cttatgcgca agggaaattg gtacgttgcg ttgttggtga ggtttttgat gttgcggttg
     4321 atattcgtaa atcgtcacca acttttggaa aatgggttgg ggtgaattta tctgctgaga
     4381 ataagcgtca attgtggata cctgaagggt ttgcgcatgg atttttggtg ttgagtgaaa
     4441 cggctgattt tttgtataag acgactaatt attatcatcc tgagagtgaa agtgggcttc
     4501 tctggaatga ttatactata gatatagcct ggcctattga aaatgaaccg ctgctttctg
     4561 caaaagatct gaaagcacgt aggtttttag aaatataaaa atttctcaga aaagttgatt
     4621 ctggatttat aatgagttta attcgtaaca gtagctggaa tatggtgggg tatgcgatcc
     4681 cttcagtggt agccatacct gctcttggtt accttgcaag gatgttaggt actcagcaat
     4741 ttggcttatt tacattagct cttgctattg taggatatgc aagcattttt gatgcaggta
     4801 taactcgagc agtgattcgt gaaatatctt tctttcgtga taactctatt gaaaaaaata
     4861 aagtaattgc cacatctata atctgtatat tttgtttggg aggggggggg gggcttatat
     4921 tttttttaat atcaccattt gcagttgaac tattaaatat aacaaccaac aattatacaa
     4981 tgatacagaa tagtattatt attgtaggtt tatgtatacc tgttttttta gtaaaccagt
     5041 tgcttatggg gatcatggaa ggtgaagaga gatttggtgc agtaaatgtt caaaaggtca
     5101 tagcaaacat ttttattgca gcaatgcctg ctgtttttgt atattttaat caaaatatta
     5161 tatatgcagt gataggatta ttatttggcc gtgttattgg aatgataatg gcaatatata
     5221 tatttaggct atatatacac gttgataaaa tacactttta taaaaatgta ttttatagat
     5281 tgctcaaatt tggtagttgg attacaatca gtaatattat aagtccagta atgacaatct
     5341 cggaccgatt tattcttgcg catttgatgg gagcgaatca tatagcattt tatacagcac
     5401 catcagaagg tattgctcgt ttaacaatta tacccgctgc attatcacga gcaatattcc
     5461 caaagttaag ctatgcaaaa agtaaaaaag aacggaaaat gtatcaacgc ttaggatatc
     5521 tggtattaat gctgacatgt ttacctgttg tactttttgt ctttactttt tcaagtgata
     5581 tattgagaat atggatgggg cctgagtttg ctgcagatga acccacactt atattaagaa
     5641 tattagttgt tggattttta tttaatgctt tggcgcagat tccattttct agcattcaag
     5701 ctgaaggaaa atctcattat actgctgcga ttcatttatg tgagctagtt ccatatatct
     5761 atttgctttt ctttatgatc aataagtatg gcatcattgg ggttgcatac gcttggcttg
     5821 ccagaacatt ggtagatttc gtgttactta tgattactaa aacgatacta gacacacggc
     5881 ttaaggactt atcgattaat ggaggataaa atggcaatag gaattgtaat agtcaactgg
     5941 aatgctggcg aacaactaaa aaattgtctt tgctcgatca ttcagtatca tagtgatgtt
     6001 gtcgataaag ttattattat cgataataat tcgagtgata attcgataga aaatatattg
     6061 atgttagaaa cagaagcgtt tgacataatt ataattcggg aaaacgaaaa tctgggtttc
     6121 ggaaaagctt gtaatatagc aagtcagaaa gtgacttcaa aatatatttt attccttaat
     6181 cctgatgctc aaatatatag tgggacgcta aagcaagtgg ttggttgcat ggaagcctgt
     6241 gatgacattg gtatctgtgg tattaaattg atcgattctt ttggtaatat ccagaggcat
     6301 tgtactgttc aacctaagct caaacattat tttaatcatg caacaggatt atctacaatt
     6361 tcttcgagac tatttccata tatcttcttg aaaaatttta actatgatag cgatggtgat
     6421 gttccccata ctatcggagc cttttactgc ataagaaata atttatttaa agagttaaat
     6481 ggttttgatg agttgttttt tgtttattta gaagatttag acctttctcg aagagttaag
     6541 cagaagggat atagaattcg ttatttagca tctgcatcgg cctatcaccg ggggggagga
     6601 acatctgaaa aagttaagtc attgaggtta ttttattcac tacgtagcag gattttattt
     6661 ggttttaaac atttttcttt attacaagca tacattttag tatttttaac tttatgtgtt
     6721 gaatttatta cacggtcaat tagatgtttg attcattttt cattatcaga ttttcataat
     6781 acatgtgaag catataagat tcttataaag gaattaccta agatattaaa aaaagggctt
     6841 gaaattaatg attgatttat tgattttctc gaaatatgga aatatggggg caagtagtcg
     6901 cttacgttct cttgactata ttgaatgctt agaacaagaa aatattcgag taacggtttc
     6961 aactcttttt gataatgaat atctcgagaa tctttataga aagaaaaaag tatcgtttat
     7021 ctctatattc aaatactata ttaagcgtct tgtaattgtt ctttttagag taaagaagta
     7081 tgatgttgtc tttatagaga aagagctatt cccctattta ccaggtatat tcgaaaagtt
     7141 aataaatatt tctcgcgttc catatattgt tgactatgat gatgctattt tccataatta
     7201 tgatatgcaa caatcttggt ttaagaaaat gatgctatca ggtaaactta aacctctctt
     7261 atctagagct gcttatgtta cggtaggaaa taggtattta gaagagttat ctagagagaa
     7321 tggtgctaaa aatgtaaaaa taattccgac agtcattaat ataaacagat atcataatca
     7381 acccacaata ttattcgatg aaataaaaat aggttggata ggttcaccaa gtactactaa
     7441 gtatttaaaa attatttggc ctgttttaga aaaattaagt tcgaaaaagc atataaagct
     7501 tattacaatc ggtgcagata agatcaatga ttgcccaatt cctctcgaac agtatccatg
     7561 gacattagac acagaatgtg aacttctgaa tgatatttcg tttggcgtaa tgccacttga
     7621 agatggatta tgggaaagag gaaagtgtgg ttataaactg atccaatata tggcgagctg
     7681 taaaccagtt atagcttcga atgttggagt aaatacagaa atcgttacac ctaaagtcgg
     7741 ttttcttgct tctgacgaaa ctgaatggtt agaggcattc aataagttca tcgataatcc
     7801 agagttgata tctttaatgg gagtaaatgc tcgacgtcga gttgaggagc attacacact
     7861 gcagattaat agtaacattt taataggttt gctcaaagac gttgttgaag gacgtcagat
     7921 ataaattttg acagtgaaca catatttaac cacatgttta tttgcataac tttaggataa
     7981 taaaatgatt attgctgtag taggtttagg ttatgttgga ttaccgctag ctgttgagtt
     8041 tggtaaatca aaccaaacaa tcggttttga tctatcagaa tcgaagataa actcatataa
     8101 agagcatatt gatccaactg gtgaggtatc tactgaagat ttgcgtaagg cagataaact
     8161 agtctgtaca actgatgcca atagtcttaa aaatgcagat tttattatta ttgctgtacc
     8221 gactccagta gatgagtctc atcagcctga ctttactcca ttgataaaat cttctgaaac
     8281 agttggtaag aatttaaaaa ggggagcgat tgttgtatat gagtcaacag tatatccagg
     8341 tgcaacagag gagatttgta ttcccatttt agaaagattt tctgggctca aatggaaaga
     8401 agatttcaat gttggttata gtcctgagag aattaatccg ggcgataaag aaagaacagt
     8461 aactaaaatt gtcaaagttg tatcaggtga tactccagtt actttgaaaa aagtccgcga
     8521 tacttatgaa aaaattatta cagctggtgt ttactgtgct aactctatta aagttgctga
     8581 agctgcgaaa gtaattgaaa acacccagag agacctcaat atagcattga tgaatgaact
     8641 ggcgatcatt tttgataaat tatctattga tactctagaa gtgcttgagg ctgcaggtac
     8701 taaatggaat ttcctgccat tccggccagg gttagttggt gggcactgta ttggtgttga
     8761 cccttattat ttgacacata aagcagatat gatcggctat catccacagg taatacttgc
     8821 cggacggcgc attaatgatt ctatggggaa gtatatagca gaacaaactg tgaagaaaat
     8881 gattgcagca aatatctcaa ttaagggttc taaaattctt gttatgggct taacttttaa
     8941 agaaaattgt cctgatctgc gtaatagcaa agtcattgat gttatcaaag aattggagtc
     9001 atacggggtc aatatcatag tacatgatcc tctagtatct agtattgatg cctataatga
     9061 atatggtgta attcttcatg aatgggatca aattgaacct tgtgatgcag ttatattgtg
     9121 tgtctgtcat aatgactata tcaatgctgg gtttgatgca attaagtcaa aattaattcc
     9181 tggtggtgta ttctcagata ttaagagtat ttggaccact gaagaagtcg aagcgcacgg
     9241 cataaatagc tggagactgt aaaatgaatt cttatgaaat aataaaaaaa aatctgttaa
     9301 catctaaaaa aaaatggcta attactggag tcgctggatt tattgggagt aaccttttag
     9361 aacaactgtt aaagttagat caggatgtag taggacttga taatttttca acggggcatc
     9421 agcataatct tgatttagtt aaagagttag tgactccagt acaatggtcg cgttttaagt
     9481 tcattaaagg tgatattcgt aatttagaag attgccgtgc tgcttgttgt gaagcacaat
     9541 acattctgca tcaggcagct ttgggatctg ttcctcgctc aatcaaggat ccgataggaa
     9601 ctaatgataa caatattact ggttttttaa atatgctggt tgctgctaat gagcagaagg
     9661 ttaagcgttt tgtctatgct gccagtagct caacttatgg aacccatcca ggtttaccta
     9721 aggtagaaga tacaattgga gacccactct ctccttatgc agtaactaaa tatgttaatg
     9781 aattatatgc tggggtgttt tcacgaactt atggacttga atctgttgga ctgagatatt
     9841 ttaatgtatt tggccctcgt caagatccta atggcgcata tgcagcagta atcccaaaat
     9901 gggtcgcgtc aatacttaat aatgaagtaa tatatatcaa tggtgatggg agtactagtc
     9961 gggatttttg ttatatcgat aatgcggtac aggctaattt gcttgcagct acgacattac
    10021 aaccagatgc agtaaacacg atttataatg ttgctgttgg acaacagaca tcattaaatg
    10081 aattgtataa atatatcgta gatgagttag tcagatatgg agttgagatt aatagtaata
    10141 acttaaaata tcgcgatttt cgtgaaggtg atgttaaaca ttcattagct aatataagta
    10201 aaataaaaaa taagttagga tatgaaccac tattcactct aaaggatgga ctagttccat
    10261 ctattcaatg gtattattct aatcattaac aagaagaggt aatgcatgag cgttacctct
    10321 tagtttaatt atatgaaaaa aattctcttt attgtaaatg ttgataagtt cttcatttct
    10381 catcgcttgc ctatagcatt agaatgtatt aaaaatgggt acgaggtaca tttgacatgt
    10441 gctatgacag attctatatc gtacttacag aaattaggga ttattaccca tccaattcct
    10501 ttatcgcgag cagggatttc aatattaaac gaaattaata catgctttca tatttacaac
    10561 actgtaaaga aaatcaaacc cgatattgtt catatggtga ctgtaaagcc agtagcttat
    10621 ggtggtttgg tatgtaagct actgaagata aagaaacgag ttgcttctat ttctggctta
    10681 ggttatgcat tcattgacca gagcattaaa gcgaaaataa ttaaaaatat agtcgtttta
    10741 ctctacagaa tttctcttaa aaataaaaat acaagcgtta tttttcagaa tttaaatgat
    10801 aaagattatt ttattaattg tggtataata aaaaagcatc aatccgtttt aatcagaggt
    10861 tctggtgttg atctagtgag atataaggtg aagccggagc cattaggtaa accagttgta
    10921 atgttagtat ctcgactatt atatgataaa ggtatccgtg agtttataga tgcagtgcgt
    10981 ttagttaaac aaaaaattga tttttccgct gtgcttgtag gaagtattga tgcaaatcca
    11041 aactctgcaa aagaaaatga aataatgcaa tggcaaaatg aaggaattat tgagtattgg
    11101 ggatatcgtg atgacattcc agaagtcttg tgccagtcta atcttgtcgt tttgccctca
    11161 tatcgtgaag gtcttccaaa atgtttgatt gaagcggcag cttgtggtcg ggcagtagtg
    11221 actacggatg tgcctgggtg tcgtgatgct attataccaa acgttacagg aatattggtt
    11281 gaagttaaag atcacataac gttagcaaat gcaatagaga cacttattat taatcccgag
    11341 ctacggcatc agatggccgt taaaggcaga gaacttgcag aacaggtatt tgatatagat
    11401 aacgtcgtta aattacattt ggaaatttat aaattcatca taccatcctg tcaacgaaaa
    11461 ataaacttat gtacctcaga atgaataatg tactttacac attgagtggt ttgttgttgt
    11521 ttttatcgat tttattgccg acaacattat caattgtaaa gattgtattg ttactattgt
    11581 tgcttttctc gtgtatgttc gcatgtaaga atgcatactc tttatcgaga caaatggttt
    11641 tattaattct attttattca gctattggaa tattgtgggt attatatggt gcaatacgtg
    11701 gaaacccagg ttatcttagt atgtcaactg tattgatctt ttatccaata atttttggga
    11761 tactatctgg attatataag gagaggctta atgaagaact atataagctt tttatttatt
    11821 ctgcatggtt cctttctgtc ataactttat tgataataat tgattttata tggttgcatt
    11881 ttggagtttt cgatatagct acaggtttat atgatttaga ttctgtagtt gtagataatt
    11941 caagtggtta ttttaaattt actataccta acatatcttc tttgattttt ttaattcctt
    12001 tcttaatttc tgccattttt tttagaaagc cacctaaagc acattatatc ttacttttat
    12061 tattatgcgt attggccatt ttgtccgggc gaagagcatt aatgttgaca atgtttttag
    12121 ctccagttct cgctgtagtt atcacctctg atcgattatc taataacaaa attttttgtc
    12181 gatttctgct tgtttttgtg ttgattggta tggcgattta cttgttttat attgcgtacc
    12241 ccgattattt tattgatcgt gttactaata tattaaattt tgatagtaat actagtaatc
    12301 tagagagaaa atatcagttt gatgctttga tggcaggatt ttatgaacat ccgctcttcg
    12361 ggaatggggc tggggccgcg gcgtcatact tgcgatcgga ttcgcaacct tgggcttatg
    12421 aattatcata cattgcattt ttatttcaat ttggtcttgt tggattcttt atttatctta
    12481 tgggaatttt attcattatt tatactctta ttcgtcgggt aaagtttgta ggaagaaaca
    12541 gcatggaatt ctgctttcta tcaggtttca taagttttat gatagcaaat gctacaaatc
    12601 cttatttgct gaagtttgat tatatgtggt tgatttttat tcctttaggt atagctaatt
    12661 caattttagt caagaaatga ccccacatcc agttttaagt tgttgcattt ctaccataaa
    12721 ataatgtgct tttaacatta aagttaaaag cacattattg aaaaaagttc tagatataat
    12781 cacttgacag gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta tggcagtgat
    12841 ggggcgcaac cttgcgctca atatcgaaag ccgtggttat accgtctcta ttttcaaccg
    12901 ttcccgtgaa aagacggaag aagtgattgc cgaaaatcca ggcaaaaaac tggttcctta
    12961 ctatacggtg aaagagtttg ttgaatctct ggaaacgcct cgtcgcatcc tgttaatggt
    13021 gaaagcaggt gcaggcacgg atgctgctat tgattccctc aagccatacc tcgataaagg
    13081 tgacatcatc attgatggtg gtaatacctt cttccaggac accattcgtc gtaaccgtga
    13141 gctttctgcc gaaggcttta acttcattgg taccggtgtt tccggtggtg aagaaggcgc
    13201 gctgaaaggg ccttccatta tgcctggtgg ccagaaagaa gcctatgaac tggttgcacc
    13261 gatcctcacc aaaatcgccg cagtggctga agacggtgag ccatgcgtta cctatattgg
    13321 tgccgatgga gcaggtcact atgtgaagat ggttcacaac ggtattgaat acggcgatat
    13381 gcagctgatt gctgaagcct attctctgtt gaaaggtggt ctaaacctct ccaatgaaga
    13441 actggcacag acctttactg agtggaataa cggtgaactg agcagctacc tgatcgacat
    13501 caccaaagat atcttcacca aaaaagatga agacggtaac tacctggttg atgtgatcct
    13561 ggatgaagcg gctaacaaag gtaccggtaa atggaccagc cagagtgcgc tggatctcgg
    13621 cgaaccgctg tcgcttatta ccgagtctgt gtttgcacgt tatatctctt ctctgaaaga
    13681 tcagcgtgtt gccgcatcta aagttctctc tggcccgcaa gcacagccag caggcgacaa
    13741 ggctgagttc atcgaaaaag ttcgccgtgc gctgtatctg ggcaaaatcg tttcttatgc
    13801 tcagggcttc tctcagctac gcgccgcgtc tgaagagtac aactgggatc tgaactacgg
    13861 cgaaatcgcg aagattttcc gtgctggctg catcatccgt gcgcagttcc tgcagaaaat
    13921 caccgatgca tacgccgaaa atccgcagat cgctaacctg ctgctggctc cgtacttcaa
    13981 gcaaattgcc gatgactacc agcaggcgct gcgcgatgtc gtcgcttatg cagtacagaa
    14041 cggtatcccg gttccgacct tcgccgctgc ggttgcctat tatgacagct accgtgccgc
    14101 tgttctgcct gcgaacttaa tccaggccca gcgcgacta
//
