LOCUS       DQ109552               12507 bp    DNA     linear   BCT 06-DEC-2005
DEFINITION  Escherichia coli O139 O-antigen gene cluster, complete sequence.
ACCESSION   DQ109552
VERSION     DQ109552.1  GI:73809588
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Wang,L., Liu,B., Kong,Q., Steinruck,H., Krause,G., Beutin,L. and
            Feng,L.
  TITLE     Molecular markers for detection of pathogenic Escherichia coli
            strains belonging to serogroups O 138 and O 139
  JOURNAL   Vet. Microbiol. 111 (3-4), 181-190 (2005)
   PUBMED   16280204
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Wang,L., Liu,B., Kong,Q., Steinrck,H., Krause,G., Beutin,L. and
            Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O139"
     gene            1140..2225
                     /gene="rmlB"
     CDS             1140..2225
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AAZ85714.1"
                     /db_xref="GI:73809589"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1140..11188
                     /note="O-antigen gene cluster"
     gene            2225..3124
                     /gene="rmlD"
     CDS             2225..3124
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAZ85715.1"
                     /db_xref="GI:73809590"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAT
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3182..4063
                     /gene="rmlA"
     CDS             3182..4063
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AAZ85716.1"
                     /db_xref="GI:73809591"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFVDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGYS"
     gene            4065..4595
                     /gene="rmlC"
     CDS             4065..4595
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AAZ85717.1"
                     /db_xref="GI:73809592"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKIFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVYDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESERGIIWDDKNINIHWPIV
                     DIPILSKKDHNNTSLL"
     gene            4922..6160
                     /gene="wzy"
     CDS             4922..6160
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAZ85718.1"
                     /db_xref="GI:73809593"
                     /translation="MSFIHILLLIILFYLPFIFLKFGLRKKITHPPTLFCAIFSFVLS
                     MAYIAHITLGFYEISYESYAIYGLGNLSFIAGGMITDALNRRNIKNSTQLVINKQEIT
                     LLKILIFTLLLYFPIAYSEFKHATPDMPLALKILRIRERGLEEQVYSTITNNMIILSS
                     CLVMYMTFIFSIKKINVYYYSLTFVLFCAYNFMTGTRAAIILISIACIFIYLLNTNKN
                     NKTSKLFLLSVGMTAIILGALVAIFMGKDGMERDESLLANLNKVVDNYFSYTVQGVIL
                     FDNYVIGREKITPNWDILSGSAEVINKITSSNIFKTDSKFSEFSHFAKDKDGNVCTIY
                     FAIYPLYGLVGVILFFLLYGTVCTILYNHGPGIVSVLVGYINATLCLNIFNEQVFINI
                     IFTIKFICFLLLIRCFERIK"
     gene            6157..7029
                     /gene="wfaI"
     CDS             6157..7029
                     /gene="wfaI"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85719.1"
                     /db_xref="GI:73809594"
                     /translation="MNVGVVIVWYNPTKAQIENVYSLANTSELKICIVDNSNDENLFE
                     KKTLSSKNISYIHNSNKGGIAGAFNRGINKLFAERTMKHIFTMDQDSELSEDYFLSMW
                     KFVRDNNCEIACPNFYDRNSKTYGTFINLTPFFYKAVNNGTTTFCISSGMIFSRKAWE
                     ILGDFDERLIIDHVDTDYCLKANNHKVTILVNYEQCLNHAIGERESHRILGVTLKPNH
                     HNYIRKYYIVRNGTYLALKYFKFSKGYFNLNILRVIHETVCVILYENDKIRKLSYMLR
                     GLRDGIKGKLGAIK"
     gene            7016..8236
                     /gene="wzx"
     CDS             7016..8236
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAZ85720.1"
                     /db_xref="GI:73809595"
                     /translation="MPLSKSIYAILSISCLLLGLYIQFLFAEFYQGDFLNLFYLGVSF
                     CSVVISMSSGGLGGFLISRFSNLSETLRLDIAVFSFTIVFSIIGVGVFCALSYTSLFV
                     LKMALHQSLIIAFLFFLYLLSLALNLIFQSYSYSKKGISGPIFYEFCSLISYILVAFL
                     LVINKEDYIICCILFCVRGYFQLILYYIFCKDKIACRLINGDEVKSFFNEIKYLMSGS
                     AYYKSEPLIDRFFLVASSHNLAAYHLISQIYNAALGLWFKISVSPLVNKLTQNTTNTI
                     VFMKLFRRGLLSQCIISIVSAIILFFTPTLHYLESINIFRPIAQNADVVYLLFIFFVA
                     SLLGQVVSNAYYSIGHHKTPVIVSCITYTLFLPAKYYLVKRYGVSGLCILVSLYHTVN
                     FIILWLMFRVKDES"
     gene            8226..9284
                     /gene="wfaJ"
     CDS             8226..9284
                     /gene="wfaJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85721.1"
                     /db_xref="GI:73809596"
                     /translation="MKVNNIVRIMAVTSGIKTPSSRFRIRQHIEQLRKYNLYVDEIIP
                     PIDKNKSVPFLSKISPKYYIPLYIPWQIIKILQRLPVLLKQTRYKYIWLNRELLTGIP
                     TLEVFFCKKIILDVDDAIWKNPPMGEITARFLAKKAYKIICGNNYLAEWFRRYNNNVY
                     VIPTAVDVHKFIPRCNKRKDIVLGWIGTHGNLKYLEKIYPTLLKILNSNRNVYLNIVS
                     DKRPEFCIDNSRANYIEWSAEDEVQNFQSIDIGLMPLDDNEWTRGKCSYKLLQHMACG
                     SLVVGSPVGMNKEILLEDNGAYPANTENEWMDTLSYLISNFEEIYCIQSKKARRFIEC
                     EYSADTIREKLARVMIDD"
     gene            9277..10434
                     /gene="wfaK"
     CDS             9277..10434
                     /gene="wfaK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85722.1"
                     /db_xref="GI:73809597"
                     /translation="MTKVIMVLPRYLPIIGGAEVQCSRLIREFNKTGKVNIVSIVTRR
                     VSNTLEKKEYIDNIPVTRIPPSGTGMLVEYIFCLLLIFYLLLNHKKYDVIHCHASSIF
                     GLSCSLVGFLLKKKVIIKISTNGEIGSMQSSSIKRCISRFLSKYCTYIALNSEGYSEV
                     NCYLSKAHKCIIPNGIDFNINHEKNLELATVIRKKLHNMYGEDIYICVFVGRFVTRKG
                     IREIYECGADLTANGKKIIFVLIGDSELQRDAINFEYDHLHNFYIAGRKKNVFPYLIA
                     GDLFISPSHKEGLPNTVLEALSVGKVCILSDILPHQEIAYEHPKNVILFKTGDAQELK
                     QTIINYLELNPHKSSLHSYVQIEDNVLLDKYSISCIAEQYCLLYKDKSNAS"
     gene            10424..11188
                     /gene="wfaL"
     CDS             10424..11188
                     /gene="wfaL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ85723.1"
                     /db_xref="GI:73809598"
                     /translation="MPRDDYKIYISIVSHNHGELIKNLDCIKNLSQEFIVVIKNNCYD
                     EVLLDYLKDSNAYLINEEYNKGFGYNNNVVFSYCRLQLNMQPSDYFIVLNPDLVIKNS
                     SIKELVNKMHCSNVKAATINLYLDDKLHDYDYSVRHFPRLYDFILSLVLGKNKTKIDK
                     SIITNPTEVDWCAGSFMALSSHVYQSIQGFDTGLFYVLRGYRSLFSFKEKNIGIIYYP
                     EITGIHKAQHKNRSFFRNILFGMLKVYSDLCLLKTG"
BASE COUNT     3837 a   2098 c   2606 g   3966 t
ORIGIN
        1 atcattgtgg ctgcagggat caaagaaatc ctcctggtaa ctcatgcgtc caagaacgca
       61 gtcgaaaacc acttcgacac ctcttatgaa ttagaatctc tccttgaaca gcgcgtgaag
      121 cgtcaactgc tggcggaagt acagtccatt tgcccgccgg gcgtgacaat tatgaacgtg
      181 cgtcagggcg aacctttagg tttgggccac tccattttat gtgcacgacc tgccattggt
      241 gacaatccat ttgtcgtggt gctgccagac gttgtgatcg acgacgccag cgccgacccg
      301 ctgcgctaca accttgctgc catgattgcg cgcttcaacg aaacgggccg cagccaggtg
      361 ctggcaaaac gtatgccggg tgacctctct gaatactccg tcattcagac caaagagccg
      421 ctggaccgtg aaggcaaagt cagccgcatt gttgaattca tcgaaaaacc ggatcaaccg
      481 cagacgctgg actcagacat catggccgtt ggtcgctatg tgctttctgc cgatatttgg
      541 cctgaactgg agcgtactca acctggagca tggggacgta ttcagttgac tgatgccatt
      601 gctgagttgg caaaaaaaca agcagttgac gcaatgctga tgactggggg acagttacga
      661 ctgcggaaag aaaatgggtt atatgcaagc gtttgtgaag tatgggctgc gtaacctgaa
      721 agaaggggcg aagttccgta aagggattga gaagctgtta agcgaataat gaaatctgac
      781 cggatgtaac ggttgataag aaaattataa cggcagtgaa gattcgtggc gaaagtaatt
      841 tgttgcgaat attcctgccg ttgttttata taaacaatca gaataacaac gagttagcaa
      901 taggatttta gtcaaagttt tccaggattt tccttgtttc cagagcggat tggtaagaca
      961 attagcgttt gagtttttcg agttaagcgc gagtgggtaa cgctcgtcac atcgtaggca
     1021 tgcatgcagt gctctggtag ctgtaaagcc aggggcggta gcgtgcatta atacctctat
     1081 taatcaacct aagagccgct tatttcacag catgctctga agtaatatgg aataataaag
     1141 tgaagatact tgttactggt ggtgcaggat ttattggttc tgctgtagtt cgtcacatta
     1201 taaataatac gcaagatagt gttgttaatg tcgataaatt aacgtacgcc ggaaacctgg
     1261 aatcacttgc tgatgtttct gattctgaac gctatgtttt tgaacatgcg gatatttgcg
     1321 atgcagctgc aatggcacgg atttttgctc agcatcagcc agatgcagta atgcacctgg
     1381 ctgctgaaag ccatgttgac cgttcaatta caggtcctgc ggcatttatt gaaaccaata
     1441 ttgttggtac atatgtcctt ttggaagccg ctcgcaatta ctggtctgct cttgatagcg
     1501 acaagaaaac tagcttccgt tttcatcata tttctactga cgaagtctat ggtgatttgc
     1561 ctcatccaga tgaagtaaat aataacgaag aattaccctt atttactgag acgacagctt
     1621 acgcgccaag cagtccttat tccgcatcaa aagcatccag cgatcattta gttcgcgcgt
     1681 ggaaacgtac ctatggttta ccgaccattg tgactaactg ttcgaataac tacggtcctt
     1741 atcactttcc ggaaaaattg attccactag taattcttaa tgctctggaa ggtaaggcat
     1801 tacctattta tggtaaaggg gatcaaattc gtgactggct gtatgttgaa gatcatgcgc
     1861 gtgcgttata taccgtcgta accgaaggta aagcgggtga aacttataac attggtggac
     1921 acaacgaaaa gaaaaacatc gatgtagtgc tcactatttg tgatttgttg gatgagattg
     1981 tcccgaaaga gaaatcttac cgcgagcaaa ttacttatgt taccgatcgt ccgggacacg
     2041 atcgccgtta tgcgattgat gctgagaaga ttggtcgcga attgggttgg aaaccacagg
     2101 aaacgtttga gagcgggatt cggaagacag tggaatggta cctgtccaat acaaaatggg
     2161 ttgataatgt gaaaagtggt gcctatcaat cgtggattga acagaactat gagggccgcc
     2221 agtaatgaat atcctccttt tcggcaaaac agggcaggta ggttgggaac tacagcgtgc
     2281 gctggcacct ttgggtaatt tgattgctct tgatgttcac tctactgatt attgtggtga
     2341 ttttagtaac ccagaaggtg tggctgaaac cgtcaaaaaa attcgtcctg acgttattgt
     2401 taatgctgct gctcataccg cggtagataa ggctgagtca gaacccgaat ttgcacaatt
     2461 actcaatgcg acaagtgttg aagcgattgc aaaagcggct aatgaagttg gggcttgggt
     2521 aattcattac tcaactgact acgtattccc tggaaatggc gacacgccat ggctggagac
     2581 ggatgcaacc gcaccgctaa atgtttacgg tgaaaccaag ttagccggag aaaaagcgtt
     2641 acaggaacat tgcgcgaagc atcttatttt ccggaccagc tgggtatatg caggtaaagg
     2701 aaacaatttt gccaagacaa tgttacgtct ggcaaaagag cgcgaagaat tagctgtcat
     2761 taacgatcag tttggtgcac caacaggtgc tgaactgctg gctgattgta cggcacatgc
     2821 aattcgtatg gcactgaata aaccagaagt cgcaggcttg taccatctgg tagccactgg
     2881 taccacaacc tggcacgatt atgctgcgct ggtttttgaa gaggcacgaa aagcaggtat
     2941 tccccttgca ctcaacaagc tcaacgcagt accaacaaca gcctatccaa caccagctcg
     3001 tcgtccacat aactctcgcc ttaatacaga aaaatttcag caaaattttg cgcttgtttt
     3061 gcctgactgg caggttggcg tgaaacgaat gctcaacgaa ttatttacga ctacagcaat
     3121 ttaatagttt ttgcatcttg ttcgtgatga tggagcaaga tgaattaaaa ggaatgatga
     3181 aatgaaaacg cgtaaaggta ttattttagc gggtggttct ggtactcgtc tttatcctgt
     3241 gactatggca gtcagtaaac agctgttacc tatttatgat aaaccgatga tctattaccc
     3301 gctttctaca ctgatgttag cgggtattcg cgatattctg attatcagta caccacagga
     3361 tactcctcgt tttcaacaac tgctgggtga cgggagccag tgggggctaa atcttcagta
     3421 caaagtgcaa ccgactccag atggacttgc gcaggcgttt attatcggtg aagagtttat
     3481 tggtggtgat gattgcgctt tggttcttgg tgataatatc ttctacggcc acgatctgcc
     3541 gaagttaatg gacacagcag ttaataaaga aagtggcgca acggtatttg cctatcacgt
     3601 taatgatcct gaacgctacg gtgtcgttga gtttgataaa aacggtacgg caataagcct
     3661 ggaagaaaaa ccgctacaac caaaaagtaa ttatgcggta accgggcttt atttctatga
     3721 taacgacgtt gtcgaaatgg cgaaaaacct taagccttct gcccgtggtg aactggaaat
     3781 taccgatatt aaccgtattt atatggaaca ggggcgttta tccgttgcca tgatggggcg
     3841 tggttatgcg tggctggaca cggggacaca tcaaagcctg attgaggcaa gcaacttcat
     3901 tgctactatt gaagaacgtc aggggctgaa agtctcctgc ccggaagaaa ttgcttaccg
     3961 taaagggttt gttgatgctg agcaggtgaa agtattagct gaacctctga aaaaaaatgc
     4021 ttatggtcag tatctgctga aaatgattaa aggttattca taaaatgaac gtaattaaaa
     4081 cagaaattcc tgatgtactg atttttgaac cgaaagtttt tggtgatgag cgtggtttct
     4141 tttttgagag ctttaaccaa aagatttttg aggaagctgt aggccgcaaa gttgaatttg
     4201 tccaggataa ccattcgaag tccacgaaag gtgttttacg cggactgcat tatcagttag
     4261 aaccttatgc gcaagggaaa ttggtgcgct gcgttgttgg tgaggtttat gatgttgcgg
     4321 ttgatattcg taaatcgtca cctacttttg gcaagtgggt tggggtgaat ttatctgctg
     4381 agaataagcg ccagttgtgg atccctgagg gatttgcaca tggttttttg gtgctgagtg
     4441 agacggcgga atttgtttat aagacgacga attattatca tcctgagagt gaaagaggaa
     4501 ttatttggga tgataaaaat ataaatatcc attggcctat agttgatatt cctatattgt
     4561 caaagaaaga tcataataac acttcattat tatgattagt aaatttggga catgattttt
     4621 taaatgccgc cttttaaaag gcggtaaaaa actactgacc ggatgcttta atagaaaata
     4681 taaaaaaata aatttcattt tacaaaattt gaatgtcaag ttagagaaat aaaactgtta
     4741 tatgttagaa atactggtca cagcatgtaa attttcttga gtcagaatga attatatgtg
     4801 tactatttct taaaaactat tgacattttt tagttggaaa taaaggttaa aaataaaaaa
     4861 ctattcatat tgagagttaa atattacgta tgctaaaaat tttcctgtca gaggatacat
     4921 tgtgagtttt attcatatct tattgctaat tattttattt tatttaccgt ttattttttt
     4981 aaaatttggt ttacgaaaga aaataacgca tccgccaact ttattttgtg caatattttc
     5041 atttgtacta tcaatggctt atatagctca cataactttg gggttttatg aaatatcata
     5101 tgaaagctat gctatttatg gattagggaa tttatcattt attgctgggg ggatgattac
     5161 tgatgcttta aatagacgaa acataaaaaa cagcactcaa cttgttatta acaagcaaga
     5221 aataacccta ctaaagattc ttatttttac gctattgtta tattttccaa ttgcatatag
     5281 cgagtttaaa catgcgacac ctgatatgcc tctcgcgtta aaaatattac gcattcgtga
     5341 acgaggatta gaagagcagg tatatagtac cataacaaac aacatgataa tattgtcgtc
     5401 gtgtttagtt atgtatatga catttatttt ctcaatcaaa aagataaatg tttattatta
     5461 ttctctgaca tttgtcctct tctgtgctta taatttcatg actggcacga gggcagcaat
     5521 tattcttatt tcaatcgcgt gtatttttat ttacttacta aataccaata aaaataataa
     5581 aaccagtaag ctttttcttt tatcggtcgg gatgactgcc ataattctag gtgctctagt
     5641 tgcgattttt atgggcaaag atggaatgga acgtgatgaa agtttattag ctaatttaaa
     5701 taaggttgtt gataattatt tttcatatac tgttcaaggg gtgattttat ttgataatta
     5761 tgtaattgga agagaaaaga ttactcctaa ttgggatata ttatcaggct cagctgaagt
     5821 aattaataaa ataacaagct caaacatatt caaaactgac tcgaaattca gtgaattcag
     5881 tcattttgca aaagataaag atggtaatgt atgtacaatt tattttgcta tttatccatt
     5941 atatggttta gttggagtaa tattgttttt tttgttatat ggaaccgtat gtacaatact
     6001 ttataatcat gggcctggca ttgtttccgt attagtcgga tatattaacg ccactctatg
     6061 tttaaatata tttaatgaac aagtgttcat caatattata ttcacaatta aatttatttg
     6121 ttttctgcta ttaatacgct gtttcgagag gataaaatga acgttggtgt tgtgattgtt
     6181 tggtataacc ctacaaaggc tcagatagaa aatgtttata gtctcgcaaa tactagcgag
     6241 ttaaaaatat gtatagtgga taactcaaat gatgagaatt tatttgaaaa aaaaacgttg
     6301 agcagtaaaa acatatcata tattcataat agtaataaag gtggcatcgc tggagcattt
     6361 aatagaggaa taaataaatt gttcgctgag agaactatga aacatatttt tacaatggat
     6421 caggactcag agttaagcga agactatttc ttaagtatgt ggaagtttgt tagagataat
     6481 aattgcgaaa tagcttgccc aaatttttat gaccggaatt caaaaacata tggtacgttt
     6541 attaatttaa ctccgttttt ctataaggct gtaaataatg gtacaacaac attttgtatt
     6601 tcatctggta tgattttttc ccgaaaagca tgggagatac tcggggattt tgacgaaaga
     6661 cttataattg atcatgtcga tacagattat tgtttaaaag ctaataatca taaagtaaca
     6721 atattggtta attatgagca gtgcttaaat catgctattg gggaacgaga gagtcatcga
     6781 attttagggg ttactcttaa accaaaccat cataattata tcaggaaata ttatattgta
     6841 agaaatggta catatttggc attaaaatat tttaaatttt cgaagggata ctttaacctc
     6901 aatattctgc gcgtaattca tgaaacagta tgtgtgattc tatatgaaaa tgataaaata
     6961 agaaaattat cgtatatgtt acgtggatta cgggatggta ttaaggggaa gttaggtgcc
     7021 attaagtaaa agtatttatg caatcttaag tatttcatgt ttgctgttag gattatatat
     7081 tcaatttctc tttgcggaat tttatcaagg agatttttta aatttattct atttgggggt
     7141 tagtttttgt agtgttgtca taagtatgtc ttctggggga ttaggcggct ttttaatttc
     7201 aagattttct aatctttcag aaacattacg ccttgatatt gcagtgtttt catttactat
     7261 tgtcttttcg ataataggtg tgggtgtttt ctgtgctctt tcatatactt cattatttgt
     7321 gcttaaaatg gcactgcatc agtcattaat tattgctttt ttattttttt tatatctttt
     7381 atctcttgct ttaaatctaa tttttcaatc atactcctac tcgaaaaaag ggatttcagg
     7441 gccaatattt tatgagtttt gtagccttat atcttatatt ctggttgcat ttcttcttgt
     7501 gattaataaa gaagattata ttatttgctg catattgttt tgtgtgcgtg gttattttca
     7561 actgattttg tactatattt tttgcaagga taaaatagct tgcaggctaa ttaacggcga
     7621 cgaagttaaa tcttttttta atgaaattaa atacttaatg agtggtagtg catattataa
     7681 atcagagcca ttaattgata ggttttttct tgttgccagt tctcataatt tagcagcata
     7741 tcacttaatt agccaaattt ataatgcagc ccttggtctc tggtttaaaa tttctgtatc
     7801 acctttagtt aataaactga ctcaaaatac aacaaatacc attgtattta tgaaattatt
     7861 ccgtcgcggt ttattaagtc aatgcataat ctctatagta tctgctataa tccttttttt
     7921 tacaccaaca cttcactatt tagaatcgat aaatatattt cgccccatcg ctcaaaatgc
     7981 tgatgttgtc tatttacttt tcattttttt tgttgcgtct cttttggggc aagtagtttc
     8041 taatgcatac tatagtattg gacaccataa aacaccggtt atagtttctt gtataacata
     8101 taccctgttt ctaccagcaa aatattatct tgtcaaaaga tacggggtct ctggactgtg
     8161 tattctagtt tctttatatc acacggttaa ttttattatt ctgtggttaa tgtttagagt
     8221 taaagatgaa agttaataat atcgtaagaa ttatggcggt tacctccgga attaaaacgc
     8281 catcaagcag atttagaatt cgtcaacata ttgaacaatt aaggaagtat aatttatatg
     8341 tagatgaaat aatccctcct attgataaaa ataagtctgt gccatttcta tcaaaaatta
     8401 gccctaaata ttatatccct ttatatatac cttggcagat aataaaaatt ctacagcgtc
     8461 tccctgttct tcttaagcaa acgaggtata aatatatatg gttaaataga gagctgctta
     8521 ctggaatacc aacattagaa gtatttttct gcaagaaaat tatacttgat gttgatgatg
     8581 ctatctggaa aaatccccct atgggagaga taacagcgag atttttagca aagaaggcat
     8641 ataaaataat atgtgggaat aattaccttg ccgaatggtt tagaagatat aataataatg
     8701 tttatgtgat ccctacagca gtggatgtgc ataaatttat acctagatgt aataagagga
     8761 aagatatagt tttgggatgg attggaacac atggtaattt aaaatacctt gaaaagattt
     8821 atccaacttt gttaaaaatt ctgaatagca atcgtaatgt atatttgaat attgtttcag
     8881 acaaaagacc cgagttttgt attgataact cacgtgcaaa ttacattgag tggtcagcgg
     8941 aagatgaagt acaaaacttc caatcaatag atattggact tatgcctctt gatgataatg
     9001 aatggacgag aggtaaatgt agctataaat tgttacagca tatggcctgt ggttcattag
     9061 ttgttggatc gccggttggt atgaataagg aaattctgtt agaggataat ggtgcgtatc
     9121 cggcaaatac tgaaaacgag tggatggata cgttatcgta cttaatatcg aattttgaag
     9181 aaatttattg cattcagtct aaaaaagcaa gacgtttcat tgagtgtgaa tattccgcag
     9241 atacaattag ggaaaaattg gcaagggtaa tgattgatga ctaaggttat aatggtgtta
     9301 ccaaggtatt tacccatcat tggtggggct gaagttcaat gtagccgcct catccgcgaa
     9361 tttaataaaa ctggtaaagt taacatagtg agcattgtta caagaagagt aagcaatacc
     9421 ttggaaaaaa aagaatacat tgataatata cctgtaacga gaatcccacc aagcggaact
     9481 ggaatgctcg ttgaatacat tttttgctta ttattgatat tctatctttt gcttaatcat
     9541 aagaagtatg atgtaattca ttgccacgct tcctctattt ttggtctttc ttgttcctta
     9601 gttggatttc tactcaaaaa gaaagtgatt attaagatct ctactaatgg tgaaattggc
     9661 tcaatgcaga gttcgtctat aaaaagatgt atttcacggt ttctatcaaa atattgtacc
     9721 tatatagcgt taaattctga gggctattct gaagtaaatt gttatttatc taaagctcat
     9781 aaatgtatta ttcccaatgg aattgatttc aatatcaacc atgaaaaaaa tttggaattg
     9841 gccactgtga ttagaaaaaa attacataat atgtatgggg aagatatata catatgtgtt
     9901 tttgtcggtc gttttgttac aagaaaaggg ataagggaaa tatatgaatg tggtgcagac
     9961 ctgactgcta atggtaaaaa aatcatattc gtgttaattg gtgattcgga attacaacgt
    10021 gatgctatca atttcgaata tgatcatctt cataatttct atatcgctgg taggaaaaaa
    10081 aatgtgttcc cctatttgat tgctggagat ttattcattt caccatcaca caaagaaggt
    10141 ttgccaaaca ctgtccttga agctctttcc gttggcaagg tttgtattct ttcagatata
    10201 cttccacatc aagagattgc ttatgaacat cctaaaaacg tcatactttt taaaacagga
    10261 gacgctcagg agttgaagca aactataata aattatctgg aattaaatcc tcataaatca
    10321 agtttgcata gttatgtgca gattgaggac aatgtcttat tagacaaata ttctatcagt
    10381 tgtatagcgg aacaatattg tctactttac aaggacaagt ctaatgcctc gtgatgatta
    10441 taaaatatat atatctatag tttcccataa tcacggtgag ttaataaaga atttagattg
    10501 tattaagaat ttatctcaag agttcatagt ggttattaag aataactgtt atgatgaagt
    10561 attattggac tatttaaaag atagcaatgc ctatctcatc aatgaagaat ataataaagg
    10621 atttggctat aataataatg tcgtttttag ttattgtcgt ttgcaactaa atatgcaacc
    10681 ttccgattat tttatagtac ttaatccaga tttggtcatc aaaaactcgt ctatcaaaga
    10741 gttggttaac aaaatgcatt gcagcaatgt caaagctgca actattaacc tttacctaga
    10801 tgataaactt catgactatg attattctgt gaggcatttt ccccgcttat atgactttat
    10861 tttatcttta gtattaggta agaataaaac taaaatagac aaatcaataa ttaccaatcc
    10921 aacagaagta gattggtgtg caggttcttt tatggcttta agtagtcatg tttatcaatc
    10981 cattcaagga tttgatacag ggttatttta tgtattgcga ggatatcgat ctttgttttc
    11041 gtttaaagaa aaaaatattg gaataattta ttatcctgag attacaggca ttcacaaagc
    11101 tcagcataaa aatagaagtt tttttcgaaa cattttattt ggcatgttaa aagtgtattc
    11161 agatttatgt ttgctaaaaa cgggttaatt aggaataagt ccaatatttg tcactaaaga
    11221 ttgatttgtg cttagaaaga aaaattaaac cagagtcttt tagatatctt ttaaagcagt
    11281 atatgctcta tccccccctg acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc
    11341 ggtatggcag tgatggggcg caaccttgcg ctcaatatcg aaagccgtgg ttataccgtc
    11401 tctattttca accgttcccg tgagaaaacg gaagaagtga ttgccgaaaa tccgggcaag
    11461 aaactggttc cttactatac ggtgaaagaa tttgttgaat ctctggaaac gcctcgtcgc
    11521 atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc tctcaagcca
    11581 tacctcgata aaggtgacat catcattgat ggcggtaaca ccttcttcca ggatactatc
    11641 cgtcgtaacc gtgagctttc tgccgaaggc tttaacttca ttggtaccgg tgtctccggt
    11701 ggtgaagaag gcgcgctgaa aggtccttcc attatgcctg gtgggcagaa agaagcctat
    11761 gaactggttg caccgatcct gaccaaaatc gccgcagtag ctgaagacgg tgagccatgc
    11821 gttacctata ttggtgccga tggcgcaggt cactatgtga agatggttca caacggtatt
    11881 gaatacggcg atatgcagct gattgctgaa gcctattctc tgcttaaagg tggcctgaat
    11941 ctttccaacg aagaactggc gcagacgttt accgagtgga ataacggtga actgagcagc
    12001 tacctgatcg acatcaccaa agatatcttc accaaaaaag atgaagacgg taactacctg
    12061 gttgatgtga ttctggatga agcagcaaac aaaggtacgg gcaaatggac cagccagagc
    12121 gcgctggatc tcggagaacc gctgtcgctg attaccgagt ctgtgtttgc acgttatatc
    12181 tcttctctga aagatcagcg cgttgccgca tctaaagttc tctctggtcc gcaagctcag
    12241 tcagcaggcg acaaggctga gttcatcgaa aaagttcgcc gtgcgctgta tctgggcaaa
    12301 atcgtttctt acgctcaggg cttctctcag ctgcgtgctg cgtctgaaga atacaactgg
    12361 gatctgaact acggcgaaat cgcgaagatt ttccgtgctg gctgcattat ccgtgcgcag
    12421 tcctgcagaa aatcaccgat gcatatgccg aaaatccgca gatcgctaac tgctgctggc
    12481 tccgtacttc aagcaaattg ccgatga
//
