LOCUS       DQ868765               15601 bp    DNA     linear   BCT 19-JUN-2007
DEFINITION  Escherichia coli serogroup O141 O-antigen gene cluster, complete
            sequence.
ACCESSION   DQ868765
VERSION     DQ868765.1  GI:115503044
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Han,W., Liu,B., Cao,B., Beutin,L., Kruger,U., Liu,H., Li,Y.,
            Liu,Y., Feng,L. and Wang,L.
  TITLE     DNA microarray-based identification of serogroups and virulence
            gene patterns of Escherichia coli isolates associated with porcine
            postweaning diarrhea and edema disease
  JOURNAL   Appl. Environ. Microbiol. 73 (12), 4082-4088 (2007)
   PUBMED   17449692
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Han,W., Liu,B., Cao,B., Beutin,L., Kruger,U., Liu,H., Li,Y.,
            Liu,Y., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2006) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O141"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABI98969.1"
                     /db_xref="GI:115503045"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1137..14065
                     /note="O-antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ABI98970.1"
                     /db_xref="GI:115503046"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVGETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQESDATAPLNVYGETKLAGEKALQEHCVKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEACKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4057
                     /gene="rmlA"
     CDS             3179..4057
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABI98971.1"
                     /db_xref="GI:115503047"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGR
                     DDCALILGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKVLADPL
                     KKNAYGQYLLKMIKGY"
     gene            4062..4595
                     /gene="rmlC"
     CDS             4062..4595
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ABI98972.1"
                     /db_xref="GI:115503048"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEVAVGRKVE
                     FVQDNHSKSRKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDKFINVEWPPY
                     ENYILSAKDKTLPPLTK"
     gene            4624..5865
                     /gene="wzx"
     CDS             4624..5865
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABI98973.1"
                     /db_xref="GI:115503049"
                     /translation="MSKLKSNLVYLTFAQAISYIFPLLTLPYLSHKLDPSDFGMLGVC
                     QAIVQYFIIVTDYGFNITTTKEISINRNNKKKISKIFSTTLLAKIFLLFVSAIGLIIS
                     GWAVKSISGYELLLICCFVGVIGNTFYPLWLFQGTETMKIPVIMSSGAKLLLLLSIFI
                     FVKDRADLNIAALLLNSGNFFAGILGLIYIKTRKLVFWYRPTLQEVYIVLLEGWPVFI
                     STVAISFYTTFNTVLLSYKVSSAEVGFYNAADKIRIAIQSMFGPITQAFYPRIVHLYK
                     VEREAAINLLNKGMLLILGLTIPSALLFFNCSDYIVAHYLSSSFSQTASYLKLLSFLP
                     IIIGIATVYCNWGLLGDGYGKVVSKIYVSFGLIHFLYATPLIIIFKVNGLILSVYITQ
                     TLITLTTIYHYYRKVYNGFNK"
     gene            5849..6946
                     /gene="wfcA"
     CDS             5849..6946
                     /gene="wfcA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfcA"
                     /protein_id="ABI98974.1"
                     /db_xref="GI:115503050"
                     /translation="MDLINRIVILHEYGEPSHYTGLVRYAELNRIPVEFRDFNFFKKI
                     LISIRRRNYMGILKAFEDLYWMLKMLFFPRLLRGSYCIVGIAPFDIMIIPMRRILSYS
                     KYIYHTSWLYWDGADHPKKTYFFSTFIRRSWRLFLKKASSVATVTDDARKSLNVNYPE
                     TRGKTNVVYHSFNDQIFRYKGNKNKRLRVTYLGRMEKYKGINTIIRLVGERPNIDFCF
                     IGSGQEVSKIKELSYEFDNLEYAGYLKNKIEIADYLNATDVLIMPSIKIDGWEELFGI
                     ALIEAMACGCVPIATKHKGPVTILGNTVFKTHLFDEDCIEKGAIEIFDYLEDNHDYLT
                     KLKEEAVKISRQYNMEAISKLWEGILSNERR"
     gene            6933..7904
                     /gene="wfcB"
     CDS             6933..7904
                     /gene="wfcB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfcB"
                     /protein_id="ABI98975.1"
                     /db_xref="GI:115503051"
                     /translation="MKEDNVYIVVLNWENALETIKCLSSLFALEYDNYRIVVCDNNSQ
                     DSSVAEIINWIENKNINLQVLSEEDIDNFDRLCDNKIYIIKNNKNYGYAGGNNVGIKF
                     ALNDKTIKYIWILNNDTTVSPTALYSLCKKMHNHPNLGICGSLLLHQNNKSIIQAAGG
                     VFNKVFCTTKHLYENVQWLELSESEKEHLNPDYIVGASMFIDKKVFETEGLLFEDYFL
                     YYEEIDFCLRVRDKYKISVDFDSHVYHKLGDSIKKGKSELADYLSIRNRLIIARRFFP
                     ISFLTVWLSLFVVMFNRIKRMQFSRAFKVLKVIFIDSWKILLKYKTV"
     gene            7906..9012
                     /gene="wzy"
     CDS             7906..9012
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABI98976.1"
                     /db_xref="GI:115503052"
                     /translation="MEYMLLCFWLAISCCSVKVSLSKINILLIFIFLSGAYSWGYDWI
                     RYREYYENVFLISDLVTWIYEPGFTFILFVNKLLNLDYHWVVVTCTLILSVNVYKYIK
                     VKNHPNFCLLFVFSIFGFMEFAEQIRQGVAISFILLAVDNLLVKKNKKFIFYVICAAM
                     FHISAIVCLLFLYLVKLLDRKNKAFIMLSLIIWAVFLSLHLMFNIYRFFGVSGFIATK
                     LKGYAESDGSESGILSAGLLLNIMVIVICLLSIQGRHLKYYLSSVFSFFVIQSKIVSI
                     AYRFSYYGYAFIYESFEWLYMANKGRLFNKLAMIIVLLVFAFKPLLNPVYLDMFNDYH
                     FFWFGFLDTLPDVNITQVKRCSTLLNHGIQYCRR"
     gene            8987..10075
                     /gene="wfcC"
     CDS             8987..10075
                     /gene="wfcC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfcC"
                     /protein_id="ABI98977.1"
                     /db_xref="GI:115503053"
                     /translation="MVYSIVDDNMKKIYIDGYFLAQRITGVQRYAEELVKNINNKGYD
                     IIILAPKNIKRTIPGVVIKRSKMFSGLLWQQFILPIQLFFLGKPIITSLSGLGPILYP
                     NKLLAIHDASLYRYPSYFSNLYRIVYKKLYPITIFFSKAIITVSEFSKQEIKELINIS
                     KDILVVNNVTPQSYEGSLRDNIYEQMEPYILVVGTLDKRKNLKNVLEAFQQSKLKGVY
                     KLLVAGGTAKSFSDNEWITNLCSDDVIFLGYVSDAELANLYNRAHFFIYMSIYEGFGI
                     PPLESVANNCPVLLSDIDVFKEIYGDSFEYANPLSVESIAMGLERVVSSDRHELYEKQ
                     RIALSKYTKENQVKQSEEIIKRYIHAAY"
     gene            10062..11210
                     /gene="wfcD"
     CDS             10062..11210
                     /gene="wfcD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfcD"
                     /protein_id="ABI98978.1"
                     /db_xref="GI:115503054"
                     /translation="MQPIKVLQFSKFYPPAVGGIEQVVYDIVQGCSKYPHIHNDVLCF
                     NHDLGQPDHEITVENLRIMRMHTAKIVASTPLSFNIFKRFLKLRDEYDLVHFHVPNPI
                     ATFASFFVKKNKYIVHWHSDIVKQKKLLCLFKPFQNIMLRRAARIIVTSDKYGRESQQ
                     LKDYSEKLITIPIGISPIQKTINQELFQALKKEFPFKKIVFSLGRLAYYKGFEYLIKS
                     ARYLNDDCVILIGGAGELKDDLQALIQKYNLHDKVRLLGRIDDVDLSTYYIFSDVFCM
                     PSIEKSEAFGVVQLEAMLYSLPIVSTKIPGSGVDWVNADGVSGITVEPKNEHAIAEAI
                     NKLLSDDSLRHSLGKNAHNRLLELFTVDRMSSQLSELYCHLILTQKAN"
     gene            11215..12624
                     /gene="manC"
     CDS             11215..12624
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ABI98979.1"
                     /db_xref="GI:115503055"
                     /translation="MKRNVTAVILAGGVGSRLWPLSREKRPKQFISIDGKNSLLSNTL
                     KRLPELNIENVIVVCNFEHRFLVAETLRKHDLLNNNIILEPCGRNTAAAIAMAALHVN
                     KIFGSEQHLLILAADHVINNIANFSKAYFDGYDLSQLNKLVTFGVVPTYPETGYGYIR
                     IGAEINKSSYEIECFVEKPDLALAEQFLATKNYLWNSGMFLFKANFFLEELRKYRDDI
                     LACCIESYDCSVIDEDFIKIPTAVFSKCASESIDYAIMEKSSARAVVPLDAGWSDVGS
                     WDSIWHLASKDSLGNVLDGDILALETHGSYIRSESRLIATYGVNDLIVVDSDDALLIT
                     SKNKSQHVKYIVELLKKKNRPEIISGLKELRYWGEITTVLSQNEYKINLLTLKPGKEI
                     SYQIHSQRIECWYILKGEASIIVDDKSFKYNASDFVLIKPGCKHKVQNLTPEPILIIE
                     IQHGNLNDECDVERLIAEG"
     gene            12629..14065
                     /gene="manB"
     CDS             12629..14065
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ABI98980.1"
                     /db_xref="GI:115503056"
                     /translation="MKSVYNISANVIRNCNIVFGTSGARGLVVDFTHDVCAAFTHAFL
                     SVIGDKYNFNKVALAIDNRPSSYEIAQACAFAIKKHGFAVEYHGVIPTPALAHYSMQK
                     NIPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIVNSEYAFSPVGVLPNLETSTQG
                     ADYYLERYVSLFNPDILKGKRIGVYEHSSAGRDLYAPLFNQLGAEVISLGRSDEFVPI
                     DTEAVSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLSAIEL
                     NIKALAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFL
                     LASDLRINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPS
                     DSSQQIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPEL
                     RCYAEASDENQAKQYVTNVLGNITSLIS"
BASE COUNT     4795 a   2576 c   3297 g   4933 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aacacatttg tcgtggtgct gccagacgtt gtgatcgacg acgcgagtgc cgacccgctg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agaaccactg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgctattgct
      601 gaactggcaa aaaaacagtc tgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag taggtaatgc tcgtcacatc gtaggcatgc
     1021 acgcagtgct ctggtagctg gaaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactaag agccgcttat ttcacagcat gctctgaagt aatatggaat aaaagagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cgcttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgatg
     1321 cagctgcaat ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg
     1381 ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtatatggt gatttgcctc
     1561 atcctgacga ggtaaataat acagaagaat tacccttatt tactgagaca acagcttacg
     1621 cgccaagcag cccttattcc gcatccaaag catccagcga tcatttagtc cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaattgctc taacaattat ggtccttatc
     1741 atttcccgga aaaattgatt ccattggtta ttctgaatgc tctggaaggt aaagcattac
     1801 ctatttatgg taaaggggat caaattcgcg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgctggat gagattgtac
     1981 cgaaagagaa atcttatcgt gagcaaatca cttatgttgc cgatcgtccg ggacacgatc
     2041 gccgttatgc cattgatgct gataagattg gtcgtgaatt gggatggaaa ccacaggaaa
     2101 cgtttgagag cggcattcgt aaaacggtgg aatggtacct gtccaataca aaatgggttg
     2161 ataatgtgaa aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt
     2221 aatgaatatt ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct
     2281 ggcacctttg ggtaatttga ttgctcttga tgttcactcc actgattatt gtggtgattt
     2341 tagtaatcct gaaggtgtag gtgaaaccgt aagaagcatt cggcctgata ttattgtcaa
     2401 cgcagccgct cacaccgcag ttgacaaagc agaatcagaa ccggagtttg cacaattact
     2461 taacgcgaca agtgtcgaag cgattgcgaa agcagccaat gaagtcggtg cctgggttat
     2521 tcactactct actgactatg tatttccggg aaccggtgaa ataccatggc aggagtcgga
     2581 tgcaaccgca ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcattaca
     2641 agagcattgt gtgaagcacc ttattttccg gaccagctgg gtctatgcag gtaaaggaaa
     2701 taacttcgcc aaaacgatgt tacgactggc aaaagagcgt gaagaattgg ccgttattaa
     2761 cgatcagttt ggtgcgccaa caggtgctga gctgctggct gattgtacag cacatgccat
     2821 tcgtgtcgca ctgaataaac cggaagtcgc aggcttgtac catttggtag ccagtggtac
     2881 cacaacctgg cacgattatg ctgcgctggt ttttgaagag gcgtgcaaag caggcattcc
     2941 cctcgcactc aacaagctca acgctgtacc aacaacagcc tatcctacac cagcacgtcg
     3001 tccacataac tctcgtctta atacagaaaa atttcagcag aattttgcgc ttgtattgcc
     3061 agactggcag gttggcgtga aacgcatgct caacgaatta tttacgacta ctgcaattta
     3121 atagtttttg catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg gggatctggt actcgtcttt atcctgtcac
     3241 tatggctgtc agtaaacagc tgttaccgat ttatgataaa ccgatgattt attatccgct
     3301 atctacgctg atgttagcgg gtattcgcga tattctgatt atcagtacgc cacaggatac
     3361 tcctcgtttt caacaactgc tgggtgatgg tagccagtgg ggccttaatc ttcagtataa
     3421 agtacaacct agtccagatg gtttggcaca agctttcatt attggagaag attttattgg
     3481 tagggatgat tgcgcattaa ttctaggtga taatatcttt tacggtcatg acctaccaaa
     3541 attaatggat acagctgtta atagagagag tggtgcaacg gtatttgcct atcacgttaa
     3601 tgatccagaa cgttacggtg tggtggaatt tgataaaaac ggcacggcaa taagcctgga
     3661 agaaaaaccg cttcaaccga aaagcaacta tgcagttaca gggctttatt tctatgataa
     3721 tgacgtcgtg gaaatggcga aaaatcttaa gccttctgcc cgtggtgaac tggaaattac
     3781 cgatattaat cgtatctata tggaacaggg gcgtttgtct gttgccatga tgggccgtgg
     3841 ttatgcatgg ctggacacgg ggacacatca aagcctgatt gaggcaagca acttcatcgc
     3901 aacaattgaa gagcgtcagg ggttaaaagt ttcctgcccg gaagaaattg cttaccgtaa
     3961 agggtttatt gattctgagc aggtgaaagt attagctgat ccgctgaaaa aaaatgctta
     4021 tggtcagtat ctgcttaaaa tgattaaagg ttattaataa aatgaacgta attaaaacag
     4081 aaattccaga tgtactgatt tttgaaccga aagtttttgg tgatgagcgt ggtttctttt
     4141 tcgagagctt taaccagaag gtttttgagg tagctgtagg ccgcaaagtt gaatttgttc
     4201 aagataacca ttcgaagtcc aggaaaggtg ttttacgcgg attgcattat cagttagaac
     4261 cttatgcgca aggaaaattg gtacgctgcg ttgttggtga ggtttttgat gttgcggttg
     4321 atattcgtaa atcgtcacct acttttggca agtgggttgg ggtgaattta tctgctgaga
     4381 ataagcgcca gttgtggatc cctgaaggat ttgcgcatgg gtttttggtg ctgagtgaga
     4441 cggcggagtt tttgtataag acgacgaatt attatcatcc tgaaagtgat agaggtatta
     4501 tttgggatga taaattcatt aatgttgaat ggcctccata tgaaaattat attctttcgg
     4561 ctaaagataa aacactacca ccattaacta aataaatgga tatcgaagat attctaggaa
     4621 attatgtcga aattaaaaag caatttagtg tatttaacat ttgctcaagc cataagctat
     4681 atatttcctc ttctgacatt gccttactta agtcacaaac ttgatcctag tgattttgga
     4741 atgttagggg tgtgtcaagc aatagttcaa tattttataa ttgtaaccga ttacggcttt
     4801 aatattacaa caacaaaaga aatatcaata aatcgaaata ataaaaaaaa gatttcaaag
     4861 atattttcca caacattatt agcaaagatt tttttgttgt ttgtctctgc aatagggtta
     4921 attatttctg gatgggctgt gaagtcgatc tctggttatg agttattatt gatttgctgt
     4981 tttgtaggcg ttattggtaa tacgttttat cctttatggt tgtttcaagg gactgaaacc
     5041 atgaaaatac ctgtaataat gtcctctggt gctaagttac ttttactatt aagtattttt
     5101 atttttgtaa aagaccgagc agatctcaat attgcagcat tattattaaa ttcaggaaat
     5161 ttttttgcag gcatattggg attaatttac attaaaacaa ggaagctggt tttttggtat
     5221 aggccaacat tacaagaagt ctatattgtt ttacttgaag gctggccagt atttatctcc
     5281 actgttgcaa ttagttttta tactacattt aacacagtac tgttaagtta taaagtcagt
     5341 tctgcagaag taggctttta taatgctgcg gataaaataa gaatagctat acagtcaatg
     5401 tttggtccca ttactcaagc attttatcct agaatcgtac atttatataa agttgaacga
     5461 gaagctgcta taaacttgtt gaacaaaggc atgctgttaa tcttggggtt gacgattcct
     5521 tcagcgttac tcttctttaa ttgttcagat tatatagtag cacattattt gagtagttcc
     5581 ttttctcaga ctgcttctta cttgaagctt ttgagttttt taccaataat tattggtata
     5641 gcgacagtat attgtaattg gggattacta ggggatggtt atggaaaagt agttagtaag
     5701 atttatgttt catttggcct aatacacttt ctgtatgcaa caccacttat aataatattt
     5761 aaagtcaatg gacttatttt gtcagtatat ataacacaaa cactgataac tttaactaca
     5821 atatatcatt actatagaaa ggtatataat ggatttaata aatagaattg tcattttaca
     5881 cgagtacgga gaaccttcac actatacggg gcttgtaagg tatgcagaat taaatagaat
     5941 acccgtagag tttagggatt ttaatttttt taaaaaaatc ctaataagca tcaggcgaag
     6001 aaattatatg ggaattttga aagcatttga ggatttatat tggatgctga aaatgctttt
     6061 ttttccaagg ttactgagag gaagttattg cattgtagga atagctcctt tcgatataat
     6121 gatcatacct atgagacgta tattatccta tagtaaatat atttatcata catcatggct
     6181 atattgggat ggtgcagatc atcctaaaaa aacatatttt tttagtacat tcatacggcg
     6241 ttcctggagg ctttttctta aaaaagccag ttcagttgcg actgttactg atgatgctag
     6301 aaaaagttta aatgtgaatt atccggaaac aagaggtaaa acaaatgtag tataccactc
     6361 ttttaatgat caaatattta gatataaagg taataaaaat aagagactga gagttactta
     6421 cttgggacgg atggaaaaat acaaaggaat aaacacaatt atcagacttg ttggcgaacg
     6481 tccaaatatt gacttttgtt ttataggcag tggacaagaa gttagtaaaa ttaaagaact
     6541 ctcttatgaa tttgacaatc tggagtatgc tggttatcta aagaacaaaa tagaaatagc
     6601 tgattatctt aatgcaaccg atgtgttaat aatgccatcg ataaaaatag atggatggga
     6661 agaacttttt ggaattgctc ttattgaagc aatggcctgt ggatgcgttc ctatagcgac
     6721 aaaacataag gggccggtaa caatattagg aaatactgta tttaagacac atttatttga
     6781 tgaggattgt attgaaaaag gtgccattga aatattcgat tatcttgaag ataatcacga
     6841 ctatcttaca aaacttaaag aagaagcggt gaagatttct aggcaatata atatggaggc
     6901 aatttctaaa ttatgggagg ggatattaag caatgaaaga agataacgtt tacattgttg
     6961 ttttaaattg ggagaatgct ttagaaacaa taaagtgtct gtcctcactt tttgcacttg
     7021 agtatgataa ttatagaata gttgtatgcg ataataattc tcaagactct tcagtcgcag
     7081 aaataatcaa ttggattgaa aataaaaata ttaatctgca agttctatcc gaagaagata
     7141 tagataattt tgataggcta tgcgataata aaatatacat tataaaaaat aataaaaatt
     7201 atggttatgc aggtggaaat aatgtgggta ttaagttcgc tcttaatgat aaaactatta
     7261 aatatatatg gatattaaat aatgatacga ctgtgtcgcc aacagcactg tatagtttat
     7321 gtaaaaaaat gcataaccat ccaaatctag gaatttgtgg atctttatta ctacaccaaa
     7381 acaacaagtc aataatccaa gctgcgggtg gagtatttaa taaagtattt tgtactacta
     7441 aacatcttta tgaaaatgtt cagtggttag agttatctga aagtgaaaaa gaacatttaa
     7501 atccagatta tattgttggt gcatctatgt ttattgacaa aaaggtgttc gaaacagaag
     7561 ggttactctt tgaagattat tttttatatt atgaagaaat agatttttgt ttgcgtgttc
     7621 gggataaata taaaataagt gtcgattttg atagtcatgt ataccataaa ctcggtgact
     7681 caatcaaaaa aggaaagagt gaattggcag attatttgtc aatacgtaat cgtttgatta
     7741 ttgcgcgtcg tttttttccc ataagttttc tgactgtttg gctttctttg tttgttgtga
     7801 tgtttaatag aataaaaagg atgcaattct cgagagcatt taaagttttg aaagttattt
     7861 tcattgactc gtggaaaatt ttactaaaat ataagacggt ctaaaatgga atatatgtta
     7921 ttatgtttct ggcttgctat ttcttgctgt tctgtaaagg tttctttaag taaaataaat
     7981 atactattga tttttatttt tctttcgggt gcttatagtt ggggttatga ttggattcgt
     8041 tatagagagt attatgagaa tgtattttta ataagtgatc tggtaacatg gatatatgaa
     8101 cctgggttta cattcatttt gtttgtaaat aagttgctca atttagacta ccactgggtt
     8161 gttgttacat gcacgcttat tttaagtgta aatgtttata aatatataaa ggttaaaaat
     8221 catcctaatt tctgtttgtt atttgtattc agcatttttg gttttatgga gtttgcagag
     8281 caaattcgac aaggtgtcgc tatatcgttt atacttttag ctgtagataa tttattggtc
     8341 aaaaagaata aaaagtttat tttttatgta atctgtgctg ctatgttcca tatctcggca
     8401 atagtatgtt tattattttt atatcttgtt aaattattgg ataggaaaaa taaagcattt
     8461 ataatgctct ctcttataat ttgggcggtg tttctgtcat tgcatttaat gtttaatata
     8521 tatagatttt ttggagtgag tggttttatt gcaacaaaat taaaaggata cgctgaaagt
     8581 gatggtagtg aatctggtat tttaagtgct gggttgctat taaatattat ggtaatagtc
     8641 atctgtctgc tttccattca aggaagacat cttaaatatt atctatcctc agtattttca
     8701 ttttttgtaa tacaatcaaa aatcgtttcc atagcttacc gattttcgta ctatggttat
     8761 gcttttatat atgagtcgtt tgaatggtta tacatggcta acaaaggtag attgtttaat
     8821 aaactcgcaa tgataattgt actccttgtt tttgctttta agccattatt aaatcctgtt
     8881 tacttagaca tgttcaacga ttatcatttc ttctggttcg gctttttaga tactttacca
     8941 gatgtaaata taactcaagt taaacggtgt tcgacactat taaaccatgg tatacagtat
     9001 tgtagacgat aacatgaaaa aaatttatat tgatggttac tttttagcac aaagaattac
     9061 aggtgttcag cgatatgcag aggaacttgt aaaaaatatt aataacaaag gatatgacat
     9121 tattatatta gctcctaaga atattaaacg aacgatacct ggtgttgtaa ttaaaagaag
     9181 caagatgttc agtggattat tatggcagca atttattcta cctatccagc ttttcttttt
     9241 aggtaaacca ataattacat ccttatctgg attaggaccg atattatatc cgaataaatt
     9301 actagccata catgatgcat ctttatatag atatccatcc tatttttcaa atctctatag
     9361 aattgtatat aaaaagctat atcctataac aatatttttt agcaaagcca taattactgt
     9421 tagtgaattt tccaagcaag aaataaaaga acttataaat atttcaaaag atatattagt
     9481 tgtaaataat gtgacaccac aatcgtatga aggttctctg cgggataata tttatgagca
     9541 aatggagcca tatattcttg tcgtaggaac ccttgataaa aggaaaaatc taaaaaatgt
     9601 ccttgaagca tttcagcaat ctaagttgaa aggagtatat aaactattgg ttgctggtgg
     9661 gacagcaaaa tctttttctg ataatgaatg gattactaac ttatgcagtg atgatgttat
     9721 ttttttgggt tatgtttcgg atgctgagtt ggctaatctc tataataggg cgcatttttt
     9781 tatttatatg tcaatatatg aaggctttgg tattccaccg ttggagtccg ttgcaaataa
     9841 ttgccctgta cttttatctg atatagatgt ctttaaagaa atttatgggg atagttttga
     9901 atatgcaaac cccttaagtg tagagtcaat tgctatgggg cttgaaagag ttgtttcgtc
     9961 ggatagacat gaattatatg agaaacaaag aatagcatta tcaaaatata ctaaggaaaa
    10021 ccaagttaaa caatctgaag agattataaa aaggtatatt catgcagcct attaaagttt
    10081 tgcaatttag taaattctat ccacctgcag taggcggaat agagcaagtg gtttatgata
    10141 ttgtccaagg atgctcaaaa tatccacata ttcataatga tgttctttgc tttaatcatg
    10201 atttgggaca acctgatcat gaaataacag tcgaaaattt gagaattatg cgtatgcata
    10261 cagcaaaaat cgttgcatcc acaccactgt catttaatat ttttaagcgg tttttgaaat
    10321 tacgggatga gtacgatctg gtccattttc atgttccaaa tccgattgct acatttgcat
    10381 ctttttttgt gaaaaagaat aaatatatcg tccattggca tagtgacatc gtaaaacaaa
    10441 aaaaactttt gtgccttttt aaaccatttc agaatattat gctgcgaaga gcagcaagaa
    10501 ttatagttac gagtgataaa tatggtagag agtctcagca attaaaggat tatagtgaaa
    10561 aattaataac gatacccata ggaatatcgc cgatacaaaa aacaataaat caggaattat
    10621 ttcaagcatt aaaaaaagaa tttcctttta aaaagattgt tttctcatta ggtcggttag
    10681 catattataa gggttttgaa tatcttatta agtcggcacg atatttgaat gatgattgtg
    10741 ttattttaat tggtggagca ggggagttaa aggatgattt acaagctcta atccaaaaat
    10801 ataatttaca tgataaagtt agattattgg gtcgaattga tgatgttgat ttaagtacat
    10861 attatatttt ttcagatgtg ttttgcatgc catcaattga aaaatctgaa gcatttggtg
    10921 tagtccagtt agaggcaatg ttatatagcc ttccaattgt atctacaaaa atcccggggt
    10981 caggtgttga ctgggtgaat gctgatggag ttagtggaat tacagtcgaa cctaaaaatg
    11041 aacatgcaat cgcagaggca atcaataaat tattatcaga tgattcttta cgacatagcc
    11101 ttggcaagaa tgctcacaat agacttttag aactttttac tgtggatagg atgagttctc
    11161 agttatctga attatattgt cacttaattc taactcaaaa ggccaattga taatatgaag
    11221 agaaatgtta cagcagtaat tttagccggt ggtgttggtt caagattatg gccactttcg
    11281 agagaaaaaa gaccaaaaca attcatatcg attgatggta aaaactcact attatcaaat
    11341 acattaaagc gccttcctga gttaaatatc gaaaatgtta ttgttgtctg taattttgaa
    11401 catcgttttt tagtcgcaga gactttgagg aaacatgatt tattaaataa taatatcatt
    11461 cttgaacctt gtggtagaaa tactgctgca gcaattgcaa tggctgcgtt acatgtcaat
    11521 aaaatatttg gttctgaaca acatttgtta atacttgctg ctgatcatgt gataaataat
    11581 attgctaact tctcgaaagc atattttgat gggtatgatc tgtcacaatt gaataagctt
    11641 gttacttttg gagtagtacc gacttaccca gagactggat atggatatat aagaatcggt
    11701 gctgaaatta ataaaagttc ctacgaaatt gaatgtttcg tcgagaaacc tgatctagct
    11761 ttagcggagc agtttttagc tacaaaaaat tatctatgga acagtggtat gtttctcttt
    11821 aaggcgaact ttttcctaga agagttacgc aaatatagag atgatatatt agcgtgttgc
    11881 atcgaatctt atgattgttc tgtaattgat gaagatttta tcaaaatccc cacggcggtt
    11941 ttttctaaat gtgcatctga atcgattgat tatgcaatta tggaaaaatc ttcggctaga
    12001 gcagttgttc ctcttgatgc aggatggagt gacgtcggtt cttgggattc gatttggcat
    12061 ttagcgtcaa aggattctct cggcaatgtg ttggatggag atattttggc attagaaaca
    12121 catggaagtt atatacgatc ggagagtaga ttaatagcca cttatggtgt taatgactta
    12181 atcgtggttg attctgatga cgcattgttg ataacgtcta aaaataaatc tcaacatgtt
    12241 aagtatatag ttgaattatt aaaaaagaaa aataggccag aaattatatc aggtcttaaa
    12301 gaacttagat attgggggga gattactaca gttttatctc aaaatgagta taaaattaat
    12361 ttattgactc ttaaacccgg aaaagaaatt tcttatcaaa ttcattcaca acgtatcgaa
    12421 tgctggtata ttcttaaagg cgaggcatcc ataatagtgg atgataagtc ttttaaatat
    12481 aatgctagtg actttgttct aattaaacca ggttgtaaac ataaagttca aaatttaact
    12541 ccagagccaa ttttaattat tgaaattcaa catggcaatt taaatgatga atgtgatgtt
    12601 gaaagattga ttgcagaagg atagtaaaat gaaaagtgtt tacaacatca gcgcaaatgt
    12661 tattcgtaat tgtaatattg tttttggaac tagtggtgct agaggtttag tggtagattt
    12721 cacccatgat gtttgtgccg cattcactca tgcgtttctt tctgttattg gtgacaaata
    12781 caattttaat aaagttgcct tagccatcga caaccgccca agcagttacg aaattgctca
    12841 ggcatgcgcg ttcgctatca aaaagcatgg gttcgctgtt gaatatcatg gtgtaattcc
    12901 aactcctgca ttggctcatt actccatgca gaaaaatatc ccctgtataa tggttaccgg
    12961 gagccacata ccttttgatc gtaatggttt gaaattctac cgaccagatg gtgaaatcac
    13021 aaaagaggat gagctagcaa tagtaaacag tgaatatgct ttttctcctg taggtgtatt
    13081 acctaatctt gaaacaagca ctcaaggtgc agactactac cttgagcgtt atgtttctct
    13141 ttttaatccc gatattttaa aggggaaaag aataggggta tatgaacatt ctagtgcggg
    13201 acgcgattta tatgctcctc tttttaatca attgggtgca gaggtcattt ccctaggtag
    13261 aagtgatgag ttcgttccga ttgatacgga agcagtaagt gatgaagatc gtatacttgc
    13321 aagagagtgg tctaaaaaat ataatcttga tgctattttc tcaacagatg gcgatggtga
    13381 tcgtccttta gttgccgatg aaaatggtga atggctaaga ggcgatattc tgggattact
    13441 ttctgctatt gaacttaata tcaaggcgtt ggctattcca gtgagttgta atacagcaat
    13501 tgaagagtct aataaatttg caagtataca acgaacgaaa ataggttctc cttatgtaat
    13561 tgcagcgttt gcagatcttg ctaagcaatt tgattcagtc gctggttttg aagctaatgg
    13621 tggttttctc cttgcctccg atttacgaat taatggcaag gaattaaaat cgttacctac
    13681 acgagatgct gtgttaccag cattaatgct cttaatagct tctcgcaata gtactatttc
    13741 tcaactgatt aataatcttc ctcagcgatt cacttggtca gatagagtta aaaacttccc
    13801 ttcagattca agtcaacaaa ttataaaaaa cgccatatcg tcacccaata atttctttaa
    13861 tagtttaggt tacgaatcat tatcctgttc cgctattgat gaaacggatg gtgcaagatt
    13921 tactttaaat aatggtgata ttatacatct ccgtccttcc ggtaatgctc cagaactccg
    13981 ttgttatgct gaggccagtg atgaaaatca ggctaagcaa tatgttacga atgtgctggg
    14041 aaatattacc tctttgattt cttgatgtta taggtatatc tacgcttata tgtgtgcgta
    14101 ggtttgatta cacgtagatg ccggtataca gaattgaaga acggtatttg ttgcattaat
    14161 gaaattcagc actacacaca ttcgtgcaac ttgagataac atctcaatca tattcaagtc
    14221 gcgcatacat cgcggtgaac accccctgac aggagtaaac aatgtcaaag caacagatcg
    14281 gcgtcgtcgg tatggcagtg atggggcgca accttgcgct caatatcgaa agccgtggtt
    14341 ataccgtctc tattttcaac cgttcccgtg aaaagacgga agaagtaatt gctgaaaatc
    14401 caggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct ctggaaacgc
    14461 ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattctc
    14521 tcaagccata cctcgataaa ggtgacatca tcattgatgg cggtaacacc ttcttccagg
    14581 ataccatccg tcgtaaccgt gagctttctg ccgaaggctt taacttcatt ggtaccggtg
    14641 tttccggtgg tgaagaaggt gcgctgaaag gtccttccat tatgcctggt gggcagaaag
    14701 aagcctatga actggttgca ccgatcctga ccaaaatcgc cgcagtggct gaagacggtg
    14761 agccatgcgt tacctatatt ggtgccgatg gtgcaggtca ctatgtgaag atggttcaca
    14821 acggtattga atacggcgat atgcagctga ttgctgaagc ctattctctg cttaagggtg
    14881 gcctgaatct caccaacgaa gaactggcgc agatctttac cgagtggaat aacggtgaac
    14941 tgagcagcta cctgatcgac atcactaaag acatcttcac taaaaaagat gaagacggta
    15001 actacctggt tgatgtgatt ctggatgaag cggctaacaa aggtaccggt aaatggacca
    15061 gccagagtgc gctggatctc ggcgaaccgc tgtcgctgat taccgagtct gtgtttgcac
    15121 gttatatctc ttctctgaaa gatcagcgtg ttgccgcatc taaagttctc tctggcccgc
    15181 aagcacagcc agcaggcgac aaggctgagt tcatcgaaaa acttcgccgt gcgctgtatc
    15241 tgggcaaaat cgtttcttac gctcagggct tctctcagct acgcgccgcg tctgaagagt
    15301 acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc
    15361 gtgcgcagtt cctgcagaaa atcaccgatg cttatgccga aaatccgcag atcgctaacc
    15421 tgctgttggc tccgtacttc aagcaaattg ccgatgacta ccagcaggcg ctgcgtgatg
    15481 tcgttgctta tgcggtacag aacggtatcc cagttccgac cttcgccgct gcggttgcct
    15541 attatgacag ctaccgtgcc gctgttctgc ctgcgaacct gatccaggct cagcgcgact
    15601 a
//
