LOCUS       EU294164               14105 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O143 O antigen gene cluster, complete
            sequence.
ACCESSION   EU294164
VERSION     EU294164.1  GI:168481263
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O143"
     gene            1569..2654
                     /gene="wfdP"
     CDS             1569..2654
                     /gene="wfdP"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdP"
                     /protein_id="ACA24757.1"
                     /db_xref="GI:168481264"
                     /translation="MKPYKLASHAIWESENKETILKLDWNESTIEPPERVKNALMNSL
                     VNNLIRYYPDVSNKDLIIKISNYVGVPKEYIQYFCSSDSAHESIIRTLINENDKILML
                     APTYDNFRTTGEAQGATICYSIFEDNYYWSLKKFESDLNKFQPQMAYLCNPNNPTGTL
                     IPKGDIHYLVEKYKGIYFIIDEAYYEFAKLTCADFVNSFNNIIITRTFSKAFSLAGMR
                     MGYIIASQDLLGAINKVRNAKNIPQLSQIAALNALDEVEYMNNYVKEVNQAKRFFYDK
                     LKNINIGQKNIVYGEGNFLVIEFKSMNDKKAFIERLKSNLIFVRDLSHMKEVELCVRI
                     TIGTKKQMLYVIRILEQMYEVKKDSII"
     misc_feature    1569..12998
                     /note="O antigen gene cluster"
     gene            2626..3309
                     /gene="wfdQ"
     CDS             2626..3309
                     /gene="wfdQ"
                     /note="unknown"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdQ"
                     /protein_id="ACA24758.1"
                     /db_xref="GI:168481265"
                     /translation="MKLKKIALFDLCETLVDFQTGDGFIYYVIDRLGICNNIKIQTLN
                     RVASSLSIFKIEQIINKIRLGESLRKRILLYMLKNIERDILNVMARDYVNEIILRRIN
                     PAIIKILNKHLKDGDEVIIISGGYDIYIQYLVKALEINGFVCTKIKFDNRGRCLGRIS
                     GKNCMGYNKVLMLKGVLSLDVSEYHITTYSDSISDLPILKMSNVGVVVSKGQAREWVR
                     QHKLFEILI"
     gene            3306..4223
                     /gene="wfdR"
     CDS             3306..4223
                     /gene="wfdR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdR"
                     /protein_id="ACA24759.1"
                     /db_xref="GI:168481266"
                     /translation="MNRKNLSMKLMYILYNIKYLLKCFVVLITPDSLIIKKQFLEYQG
                     YPLGLNNPKALNQKLQWLKLNDRTLLHTICADKIRVREYVENRLGTRDNIIDLLKVYD
                     TPFDITFESLPQGKFVIKSNHYCGDYFIVRDKNLLNIDEVKWKFIRTLNCNIYHHGRE
                     WQYKNIDRRIIVERLLEDEAGKVPNDIKIHCFNGEPKFIYVSLDREGGNYRSIYDTSW
                     NMLDFCWARRGKDLSKFNVKNISKPSQLENALNISKKLSQGFKYLRVDLYLVQEKIYV
                     GELTFHQGSGYDKITPFEWDERLGKELKL"
     gene            4220..4666
                     /gene="wfdS"
     CDS             4220..4666
                     /gene="wfdS"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdS"
                     /protein_id="ACA24760.1"
                     /db_xref="GI:168481267"
                     /translation="MSIFSRIQLIWYKLCFICEFGVKRYKQSHIYGPYKIVEPSNLKF
                     GNSFSINDFVYINASGGVILGHNVVLSAGSKILSTGLVFEKKIFKYKKDKVVIKDNVQ
                     LGAGAIVLPGVEICSNIIVGAGAIVTKNLEEPGVYVGQPARKIKDV"
     gene            4659..5864
                     /gene="wzx"
     CDS             4659..5864
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACA24761.1"
                     /db_xref="GI:168481268"
                     /translation="MSSFLRSIISVSALKLVDYIVPIIIMPLVLTRFNLEGYGVYSYV
                     LTISTFISFIYDACLNTVGVQQLNKLKGNEIAQQSFVISAIFFKLVFFFVCAMVFIII
                     SETAGKSDYFCVVIFSFGFGMLNSWYYIAKDNFRFIIIVSLVVKILAVLFVLYYAFEL
                     HMFIFIQAIASALVGLISLIKIKNDNRLGKISFRHIKNYLKNIKHMYIYSGVNALIQP
                     IINSAILASGNASLLGIYNVVMRFVTVSVTFSNSIITVLNKCNSEVFYSVGHKTNYVE
                     VKYRSQLLKLLFYSIVVFICVAAISYVSIYLTKGEISRFFIFLIFSFSLAVIPSILNQ
                     YLTQLIHMIHSSKNIVKYVICSNVLSLISIFLFSSLFPYYYIVYAVVLSPIIITICCL
                     WDLKCKNLL"
     gene            5846..6739
                     /gene="wfdT"
     CDS             5846..6739
                     /gene="wfdT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdT"
                     /protein_id="ACA24762.1"
                     /db_xref="GI:168481269"
                     /translation="MQKLAVIMTYYNESYIILKKAVDSTLQDLERLEKKNVKYKLIIG
                     VDRRNEDIPHVESLYLKEVSARNDVCVVFFNENEGLAASLNKLIFGACNGYDFIARMD
                     ADDIVINGRFKKQLEYLNKHSDVSLVGGQAVVIDENDNVVRDYFNMNPVDFSWGNEII
                     HPTWLLRSELYYKLGGYRRLDAAQDYDFLCRAVLSGCIVRNIDDVVLFYRIRHGSIGS
                     CKKFIQIKNKTILSKFFRKNEVAPIHLFNATNFSVEFFLFSVIESIKIKHNIVGKCIG
                     WLSPLHAKNYYLNLRKRMGKK"
     gene            6736..7851
                     /gene="wfdU"
     CDS             6736..7851
                     /gene="wfdU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdU"
                     /protein_id="ACA24763.1"
                     /db_xref="GI:168481270"
                     /translation="MRVLVFGPMISGHISNWIHGSKEYIDYRIVTLHYDKSISENVGI
                     GEIYKIPRLTKTKFDFIYAPVFLLFYFFLLKPELVHVHFFSSYGLISCVLPRKVKKIL
                     SVWGSDINNTIKKRGILGKIYVKAMKSFCVVNSPAKHITNKIISYFNVNQERIHTFQY
                     GVDVGFFSDEKTSSIRDDKCITITSVRNWDNIYNIESFLEAVIDMKDYDCKGYFFNIN
                     ILGRSSSKTTHDKIIELTKKCEGDNYKVNLIGFIQKEELRSILHKSDFVLSIPSNDGA
                     PLSLMEGVLCNCFPIVSDIDANRELLGNHAMYLNLHDDIDMKSVFSRCVKLIDSNQYR
                     CSIEYNRDTIIKMFDINKNTKRMVDIYYRVVSQKEFL"
     gene            7848..8930
                     /gene="wzy"
     CDS             7848..8930
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACA24764.1"
                     /db_xref="GI:168481271"
                     /translation="MKVFFDVFLLFLFVFAFSIPVFFNSGYLIYIFSLIYLITNIRIR
                     TVFFSLISTRIFFIAFLSLACMLFFSCISLVLNGTGDLSIMGVIFNNFAALFSSVIIA
                     SHLIQHNNDNVLKLFFLIALLQSFFIILMMFFPSINAYIQSIIRTKEEIEFMSNTYGG
                     IRGLGISGSVAFGLAIIMSLLSFLSIVWFNEEDCNIPFFFKLFFIILLLFASISAGRT
                     AVLGFVLGGVYSFICSDYKKKFYRALQILIYVSLFLFIFWRFFLNEHVYDILKKYSDY
                     SFQMLYYYLDSGKLSTTSTDALFNMYFPLTEKQIFIGDGYYTVKGWDILSRDRCWIYA
                     VSIIFGCGTIGYFLFFIFYFFDYLCC"
     gene            10861..11874
                     /gene="gne"
     CDS             10861..11874
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACA24765.1"
                     /db_xref="GI:168481272"
                     /translation="MTILVTGGAGYIGSHTVLSLLSYGKNVVVLDNLSNSSSKSLERV
                     EKICGRRPLFYHGDVRDRKKLKHIFQYHDITDVIHFAGLKAVAESVSNPLEYYDHNVN
                     GTIVLLEEMMRAGVISFIFSSSATVYGKPETIPLKEDSSVGGTTNPYGFSKLIVERVL
                     KDIALAEPTMRITILRYFNPVGAHSSGLLGEDPNGVPNNLIPYITQVAIGNLKYLSVF
                     GNDYPTPDGTGIRDYIHVMDLAEGHIAALEHRNFGGNYKVYNLGTGVGYSVLDVINTF
                     KRCTGIDIPYCFKPRRVGDIAECWSDPSLAFLELGWSSRYDLDTMIIDSWRWQKNNPK
                     GYK"
     gene            12195..12998
                     /gene="wfdV"
     CDS             12195..12998
                     /gene="wfdV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdV"
                     /protein_id="ACA24766.1"
                     /db_xref="GI:168481273"
                     /translation="MSTDKYLVSVITPTYNSERTIRCTIDSVLSQSYNNIEMIIVDDC
                     SSDNTVSICREYQKKDSRVVLISNEKNYGAGMSRNIAISKAKGRFIAFLDSDDFWHKE
                     KIKKQIDFMLSNNYAFTYTSYQKVSFSGKLLSQINPVSKVNYSELLKTNVIGCLTAVY
                     DTSSLGKMFMPTIRKRQDMALWLSILDKIDYAYCLNETLAYYRVGTDTLSSNKLKIIF
                     SQWAFYRKYLKFGLFKSLYYFSHYAVNAVAKHHINPLCLFFKRHFASWR"
BASE COUNT     4568 a   1854 c   2722 g   4961 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtaaagcgt
      121 caactactgg cggaagtaca gtccatttgc ccgccgggcg tgaccattat gaacgtgcga
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacggcctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcatcggc gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc aggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaattggcga aaaaacagtc cgttgatgcc atgctgatga caggtcacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactgcgtaa cctaaaagaa
      721 ggggcgaagt tccgtaaagg tattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg ctgtgaagat ttgcggcgaa ggtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaaagag ttagcaatag
      901 gattttcgtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcatataag agaaaacatg
     1081 tggcactgat tttttactaa acaattggcg ataaatattc aataatggag attttcctta
     1141 acatcaaacg ctattatttt atatattata aatgtccaaa tactcagtat atatacaaaa
     1201 agagtaagat aaaacttaaa gatttaataa ggtgtctcta tatttctgac tacagacaag
     1261 catggaaagt ataatatatt gaatattctg tgtctgatta cttatgctct caatttgcag
     1321 aagttagtga agatattcag tatgtggaat agatatatat tttttttgac acaatatgtc
     1381 gattattctt cacgagatta tattaagaat aaaataaatg tatgggctta tatacgaatt
     1441 atgaaacata ggagcaattc tgaaaattat ctaagataag atattgtgtt ttcttgagca
     1501 tgtttaaatt ctttaaatgt tatgcacaga acgtaatgga gtaaaaattg aataagttcg
     1561 taaaaaaaat gaaaccatat aaacttgcat cccacgctat atgggagagt gaaaataaag
     1621 aaacaatatt aaaattagat tggaacgaat ctactattga accgccagaa agagtaaaaa
     1681 atgcattaat gaattcgttg gtaaataatc taattaggta ttatccagat gtgagcaata
     1741 aagatttaat aataaagata tcaaactatg tgggggtgcc aaaggaatat atccaatatt
     1801 tttgtagttc agactcagca cacgagtcta tcattcgtac attaattaat gaaaatgata
     1861 aaatattgat gttagcacct acgtatgata atttcagaac aacaggtgaa gctcaagggg
     1921 caacaatttg ttattcaatt tttgaagata attactattg gtcattaaag aagtttgaaa
     1981 gcgatctcaa taagtttcaa cctcaaatgg cctatttatg taacccaaat aatcctacag
     2041 gtactcttat tcctaaagga gatatacatt atcttgtaga aaaatataaa ggaatatatt
     2101 ttattatcga tgaagcctat tatgaatttg caaaattaac gtgtgctgat tttgtaaact
     2161 cctttaataa cataattatt acgcgaacat tttccaaagc attttcattg gcagggatgc
     2221 gaatgggata tataatagcg tcacaagatt tgttaggtgc aataaataaa gtcagaaatg
     2281 caaaaaatat acctcagcta agtcagattg ccgctttgaa tgcattagac gaagttgaat
     2341 atatgaataa ttatgttaag gaagttaatc aggctaagcg ttttttttat gataagttga
     2401 aaaatataaa tataggtcaa aaaaatattg tatatggaga ggggaacttt ttggttattg
     2461 agtttaaatc gatgaatgat aaaaaagcat tcattgaacg tttaaaatct aatttaatat
     2521 ttgttcgtga tttatctcat atgaaagagg tcgagttatg tgtcagaatt actattggaa
     2581 cgaaaaaaca aatgttgtat gtaatcagaa ttttagagca gatgtatgaa gttaaaaaag
     2641 atagcattat ttgatctgtg tgaaacctta gttgattttc aaactggcga tgggtttata
     2701 tactatgtaa tagatcgttt ggggatatgt aataacatca aaatacagac tttaaatagg
     2761 gtggcatctt cattatccat tttcaaaatc gaacaaataa taaataaaat aagattgggg
     2821 gaaagcttaa ggaaaagaat tttattgtat atgttaaaaa atattgaaag ggatattctt
     2881 aatgtaatgg ctcgtgatta cgttaatgaa attatattgc gcagaattaa tcctgccata
     2941 attaagatat tgaataagca tttaaaagat ggagatgagg ttattattat ttctggaggc
     3001 tatgatattt atattcaata tttagttaaa gcattagaga ttaatggctt tgtatgcaca
     3061 aaaattaaat ttgataatag aggcaggtgt ttagggagaa ttagtggcaa gaattgcatg
     3121 ggatataata aagtattaat gttaaaaggt gttttgtcgt tagatgtttc tgagtatcat
     3181 ataacaactt actctgattc tattagcgat ttaccaatat taaaaatgag caacgttggg
     3241 gtagtagtat cgaaaggtca ggcaagagaa tgggtaagac agcataaatt atttgagatt
     3301 ctaatatgaa tagaaaaaat ctttccatga aattgatgta tatattgtat aatataaagt
     3361 atttattgaa atgctttgtc gttctgatta ctccagatag cttgattata aaaaagcagt
     3421 ttcttgaata tcagggatac cctctcggat tgaacaatcc gaaggcatta aatcaaaagt
     3481 tacaatggtt gaaactaaat gacagaacac tattacatac aatatgtgct gataaaatta
     3541 gagtgcgaga atatgtggaa aataggctgg gaacgagaga taatataatt gatttgttaa
     3601 aggtttatga tacccctttt gatattacct ttgagtcttt accacagggt aagtttgtta
     3661 taaaatcgaa ccactattgt ggcgattatt ttattgttag agacaagaat ttattgaata
     3721 ttgatgaggt taagtggaaa tttattagaa cacttaattg taatatttac catcacggaa
     3781 gagaatggca atataaaaat attgataggc gtataattgt tgaaaggtta ttagaggatg
     3841 aggcagggaa agttcctaat gatataaaaa ttcattgttt taatggagaa ccgaaattta
     3901 tttatgtgtc gttagatcgt gagggaggga attatcgttc tatatatgat actagttgga
     3961 atatgttaga tttttgctgg gcgagaaggg gaaaagatct ttcaaaattt aatgtgaaga
     4021 atattagtaa accaagtcag ttagaaaacg ctctaaatat atctaaaaaa ctaagtcaag
     4081 gtttcaaata tcttagggtt gatttatatc ttgttcaaga aaaaatatat gtaggcgaat
     4141 taacatttca tcaaggatcg gggtatgata agataactcc atttgaatgg gatgaaaggc
     4201 tcggaaagga gttaaagcta tgagcatatt ctctagaatt caattaatat ggtataaatt
     4261 atgttttatc tgtgagtttg gagtaaaacg ttataagcaa tcccatattt atggtcctta
     4321 taagatagta gaaccatcga atcttaaatt cggtaattcc ttttcaatta atgattttgt
     4381 gtatataaat gctagtggag gagtcatttt aggccataat gtagtactgt ctgctggtag
     4441 taaaattctc agtacaggtt tggtatttga aaagaaaatc tttaaatata aaaaagataa
     4501 ggtagtaata aaagataatg ttcagttagg tgctggagca attgttttac cgggagtgga
     4561 gatctgtagt aatattattg ttggtgccgg agcgatagtt acaaaaaact tagaggaacc
     4621 aggtgtatat gttgggcaac cggctagaaa aataaaagat gtctagtttt ttaagaagta
     4681 ttatctctgt aagtgctcta aagctagtgg attatattgt cccaataata attatgccgc
     4741 tggttttaac tagatttaat ttagagggat atggagttta ttcgtatgtg ttaactatat
     4801 caacttttat ttcatttatt tatgatgctt gtctaaatac agtaggcgta caacaattaa
     4861 ataagcttaa gggtaatgaa attgctcagc agtcgtttgt tatttcagcg attttcttta
     4921 agttagtgtt tttttttgta tgtgcgatgg tgtttattat aatttcagag actgcaggga
     4981 agagtgatta tttttgtgtc gtaatattct cgtttggatt tggcatgctt aatagttggt
     5041 actatatagc aaaggataac tttaggttta taataatagt gtccttggta gttaaaattt
     5101 tagcggtttt atttgtttta tattatgctt ttgaattaca catgtttatt tttattcagg
     5161 cgatagcatc tgctctggtt ggacttatta gtttgataaa aataaagaat gataataggt
     5221 tgggaaaaat atcatttaga cacattaaaa attatttgaa aaacattaag catatgtaca
     5281 tatatagcgg ggtaaatgca cttattcagc ctataattaa ttctgctata ttggcatcag
     5341 gaaatgctag cttgttaggg atatataacg tcgttatgcg atttgtgaca gtatccgtaa
     5401 cattttccaa ttcaattatc actgtcttaa ataaatgtaa ttcagaggtt ttttattccg
     5461 tagggcacaa aactaactat gtggaagtaa aatacagaag tcagctactc aaattgttgt
     5521 tttacagcat cgttgttttt atatgtgtgg ctgcaatatc atatgtctct atttatttga
     5581 caaagggaga aatatctcgt ttttttattt ttctaatatt ttcatttagc ctagctgtta
     5641 tcccaagtat actcaatcaa tatttaactc aattaattca tatgatacac tcatcaaaaa
     5701 atatagtaaa atatgtgatt tgttcgaatg tattatcgtt aatatctatt tttttatttt
     5761 cttcattatt cccatattat tatatagtat atgctgtagt tctatcgcca attattatta
     5821 caatttgttg tctgtgggat ttgaaatgca aaaacttgct gtaatcatga cttactataa
     5881 tgagagttac ataatactaa aaaaggcagt agacagtact cttcaggatc tggaaagatt
     5941 ggaaaagaag aatgtgaaat ataaattaat aattggagtc gataggagaa atgaagatat
     6001 tcctcatgtt gagagtttat atttaaaaga agttagtgcc agaaatgatg tgtgcgttgt
     6061 tttttttaat gagaacgaag ggttggcggc aagtttaaat aaattaattt ttggcgcgtg
     6121 taatggctat gattttattg ccagaatgga tgctgatgat attgttatta acgggcgttt
     6181 taaaaagcaa ttagaatatc tgaataagca ttctgatgtc agtttagtcg gtggacaggc
     6241 tgttgttatt gatgagaacg ataatgttgt tagggattat tttaatatga acccagtaga
     6301 tttttcgtgg gggaatgaaa taattcatcc aacttggttg ttaaggtcag agctttatta
     6361 taaattaggt ggttacaggc gtttagatgc tgctcaagat tatgattttt tgtgtagggc
     6421 tgtattgagt ggatgtattg taaggaatat agatgacgtg gttttatttt ataggataag
     6481 gcatggctca attggcagtt gtaagaagtt tatccaaata aaaaacaaga ctattttatc
     6541 taaatttttc agaaaaaatg aagttgcacc tattcattta tttaatgcaa ccaacttttc
     6601 agttgagttt tttttattct ctgtcattga atcaataaaa atcaagcaca atattgtggg
     6661 gaaatgtatt gggtggttgt ctccactcca tgctaaaaat tattatttaa atttaagaaa
     6721 acgaatgggg aaaaaatgag agtacttgtt tttggcccta tgatatctgg tcatattagt
     6781 aactggattc atggttccaa agaatatatt gattatagaa ttgtcacact acactatgac
     6841 aaaagtattt ctgagaatgt tggaatagga gagatatata agataccaag acttacaaaa
     6901 actaaatttg attttatata cgcacctgtt tttcttcttt tttatttttt tttattaaag
     6961 cctgagttgg ttcatgtcca tttttttagt tcttatggtt taatttcatg tgttttgcct
     7021 agaaaagtaa agaagatact ctcagtatgg ggaagtgaca ttaacaatac tataaaaaag
     7081 agagggatat taggaaaaat atatgtaaaa gcaatgaaaa gtttttgtgt tgtaaattca
     7141 cccgccaagc atataacaaa taaaataata tcgtatttca atgtgaatca ggagcgaata
     7201 catacttttc aatatggtgt agatgtgggg ttcttttcag atgaaaaaac gtcaagcata
     7261 agggacgata aatgtataac aataacttct gtaagaaatt gggataatat ttataacata
     7321 gagtcctttt tagaagctgt tattgatatg aaagattatg attgtaaagg gtactttttt
     7381 aatatcaata tactaggccg ctcatcgtca aagaccacac atgataagat aatagaactc
     7441 acaaaaaaat gtgagggaga taattataaa gtaaatttaa tagggtttat tcaaaaagag
     7501 gaattaaggt cgattcttca taaatctgat tttgttctta gtattcctag caatgatggt
     7561 gctccattat ctttaatgga gggcgtttta tgtaattgtt ttccgatagt atcagacatc
     7621 gatgcaaaca gagagctatt aggaaatcat gcaatgtatt tgaatctgca tgatgatatt
     7681 gacatgaaat ctgttttttc aaggtgtgtg aaattaatag atagtaatca atataggtgt
     7741 tcgattgaat ataaccgaga tacaataata aaaatgtttg atataaataa aaacacaaag
     7801 cgtatggttg atatttatta tcgggtcgtg tcacaaaagg aatttttatg aaggtttttt
     7861 ttgatgtatt tttgttgttt ttatttgtgt ttgcttttag catccctgtt ttttttaatt
     7921 caggctatct tatatatatt ttctccttga tttatttaat tacaaatatc aggataagga
     7981 ctgttttttt tagtctaata agtacaagaa tattttttat tgcgtttttg tctttggcct
     8041 gcatgctctt tttttcttgt atttcattgg tattaaatgg tacaggagat ttatctatca
     8101 tgggggttat ctttaataat ttcgctgcat tattcagctc tgttataatt gcgtcgcact
     8161 taatccagca taacaatgat aatgtgttga aattgttttt tctaattgcc ctcttacaat
     8221 ccttttttat tattctgatg atgttttttc ccagtataaa tgcttatatt caaagtatta
     8281 ttagaacaaa agaagaaata gaatttatga gtaatacata tggaggcatc aggggattag
     8341 gtatatcagg ttctgttgcc tttggtcttg ctataattat gtcattatta tcttttcttt
     8401 caattgtttg gtttaatgag gaagactgta atattccttt cttttttaaa ttatttttta
     8461 tcatactctt gctgttcgcg tcaatttctg ctgggcgtac ggctgttttg gggtttgtcc
     8521 tcggaggggt atatagtttt atttgctctg attataaaaa gaagttttat cgggcgcttc
     8581 aaattttgat ttatgtatct ctgtttttat ttattttttg gagatttttt ttaaatgaac
     8641 atgtttatga tatcttgaaa aaatattcgg attattcttt tcaaatgtta tattattatt
     8701 tagattcagg aaagttatca acaacatcta cagatgcatt atttaatatg tattttccgt
     8761 taacagaaaa acagatcttc attggtgatg ggtactatac cgttaaagga tgggacatat
     8821 tatctcggga ccgatgctgg atttatgcgg ttagcattat ttttgggtgc ggtaccatcg
     8881 gttatttttt attttttatt ttttattttt ttgactattt atgctgctaa tagcatcaaa
     8941 cctaaaatag ttgtattaat gattacgata ctcagttttt cttttcatta taaaggcgag
     9001 gttatatttt tgaatgttgc atatatgaaa ataatatata tatatatatt ttcatctttt
     9061 tatatttgtc ataaaaaaag attaataaga aggggttcgg ttaatgcatg attattattt
     9121 taattgcagg tttttattaa caaacaatag tagtaacttg ttaattgttg acatattatc
     9181 ttattctaga tatctgtaaa acaaattggt ttttgccttt gtttttatgg gtatttaaga
     9241 ctatagggta atagcgcaac aatttaattt aagttttttg agaactgtaa gaatagtgaa
     9301 ctacatagat cacaaatcta taactgaacc aggtgataat cccatgaata caatctataa
     9361 taaataatgt tttggattgg taagtgttat aaatttacgt ggaaattcta tttgcgtgtt
     9421 tatgttgcta tttcatggaa tggttttatt gccattgctg tagtgttatt ttgaactaat
     9481 ttatttggtg aaagattact gagtaatctt ttgatactaa atcgattgtg ttttatatct
     9541 ttgaattaaa tatttttgcc aaagcactcc ttcactctta tatattcaga tactccttgt
     9601 gttataagag taaggaatat aaattggaat ttggaaaaag gcgtgacata tttctgttgt
     9661 ctttggttat gtacacgatg ggcagagtgt aagttttcag agatgttgca attgtctcca
     9721 gataagaaag gagagattgt tatgttgaac caacatatcg gcgtcaggtg tttcgggcat
     9781 taccgtgcaa caatctatgc gatggaatac aatgccatgc ctgatattgc aaaattaaga
     9841 atgaaaaccc tgcattttag ggaaagacat ggaatcaatg catccgcaga aaatttttgt
     9901 gtatcaatac gcgtttaata tataggctcg gaagtgctta tctcaagtag taaatctttc
     9961 tagtatgtct tacaaaacta tgacatccag aaattttgaa agaaatatga tgaattagaa
    10021 taggacttct caaccttgga aaagaacaga tttttgaccg tctgaatctg tagtgtgaac
    10081 agtagcattt ggcctgcccg agcgtctcca ctattggagg aatgagctag gcagcacatg
    10141 ataaaatgtg gctaatactg gtgtaactgg gttcccagac ttcaagtcca aggtactatt
    10201 agcccgctaa agaccggtga gcttatattg agctcagaat cggcgttatg ttattaccgt
    10261 gattgacgaa cgcagcaact acgctttggc ggtggcggta acgtcgctta acagtgatgt
    10321 aatcatgagt ttcttctgcc atcaagctcg gttgttacct gttggaatca accagaatat
    10381 acccgattat ggggaagagt tcctgggaaa atttgacaaa aagctacagg aagccgctat
    10441 caggcaccaa taggcttatc tcgatacgcc gaaaatgaac accatctgtg agcgctttaa
    10501 ccgatcactg agatagccgt ttattgagtt taatgaagaa ttgctctttg aaaatctgaa
    10561 tatgtcaatc ataaacaggc tcaatattgc atactgtaca agaataaaag gcctcataaa
    10621 ttgctcgaat tactgacacc actagaacat aatttacacg aaaaaaatgc aatatagtgt
    10681 tagactcata caatatgtcg agatgtttag aaaaataacg tatacttgca tattgtattt
    10741 ttatagtaac attgggctta actattctaa atttttattc tttcacagtt tatactattt
    10801 ataatgtgtt tatattcttg ttttatatcc taggcagata gatgaaatat aaggagtttt
    10861 atgactattt tggttactgg tggtgcaggt tacattggtt cacatactgt attatcctta
    10921 ttatcatacg gaaaaaatgt ggtggtgttg gataatctat cgaactcatc gtctaagtcg
    10981 ttagaaaggg tcgaaaaaat ttgtggacgt aggcctctat tttatcatgg tgatgtgcgt
    11041 gataggaaaa aactcaaaca tattttccag tatcatgata ttacagatgt tattcatttt
    11101 gcaggattaa aagccgtcgc tgaatctgtt tcaaatccat tggaatatta tgatcacaat
    11161 gttaacggaa ctattgtttt acttgaagaa atgatgcgcg ctggcgttat tagttttatt
    11221 tttagctcct ccgcaacagt gtatggtaag cctgaaacta ttcctttgaa ggaagatagt
    11281 agcgttggcg gaacaactaa tccatatggc ttttcaaaac ttatagtaga gagggttttg
    11341 aaggatatag ctttggctga gccaacaatg cgtattacta ttttgcgtta tttcaatcct
    11401 gtaggagccc actcttctgg tctattaggc gaagatccta atggtgttcc aaataattta
    11461 attccttata ttactcaagt ggctattggt aatctaaaat atttatcagt attcggaaat
    11521 gattatccta cacctgatgg aacaggcatt agagattata ttcatgttat ggatttggct
    11581 gaagggcaca tagctgcttt agaacaccgt aattttgggg gaaattataa agtatataat
    11641 cttggtacag gggttggtta ttcagttctt gatgtgataa atacctttaa acgttgtact
    11701 ggaattgata tcccttattg tttcaaacct cgtcgagttg gtgatatagc tgaatgttgg
    11761 tctgatccat ctctagcatt tttagaatta gggtggtctt ctcgatatga tttagataca
    11821 atgataattg attcttggcg ttggcaaaaa aacaatccca aaggatataa ataagacgat
    11881 tttaatgtgc tattttattc actgtcaata tttttataaa accatctttg ctttgctttg
    11941 ctttgctttg ctttgctttg ctttgctttg ctttgctttg ctttgctttg ctttgctttg
    12001 ctttgctttg ctttgctttg ctttgctttg ctttgtagtt actctcttaa ttaacggctt
    12061 tttatattaa ggtgggatgt taatgaaata cttgataaat atttaatatg tgaaatttct
    12121 taataagcta ccaaggatgg atatgataaa catgacatta tataatgaaa cttatgtaag
    12181 tttgagatga ttctatgagt accgataaat atttagtttc agtcataaca cccacatata
    12241 attctgaaag aacgattaga tgtacaattg attctgttct atctcaatca tataataata
    12301 tagaaatgat tattgtcgat gattgttcgt cagataatac agtgtctatt tgcagggaat
    12361 atcaaaaaaa agattcaaga gttgttctca tttcaaatga gaagaattat ggggctggta
    12421 tgtcaagaaa tatagcgatc agtaaagcta aaggtcggtt tatcgctttt ttagatagtg
    12481 atgatttttg gcataaagag aaaataaaaa aacagataga ctttatgctt tcaaataatt
    12541 atgctttcac ttatacctca tatcaaaagg ttagttttag cgggaaactt ttatcccaaa
    12601 ttaatcctgt ttctaaagtt aattattctg aattattaaa gactaatgta ataggttgct
    12661 tgacagctgt atatgatact tcatctcttg gtaaaatgtt tatgccaaca atcaggaaaa
    12721 gacaggacat ggcactatgg ctgagcattt tagataaaat tgattatgca tattgtttga
    12781 acgaaacact ggcctattat cgagtaggca ccgatacact ctcgtcaaat aaattaaaaa
    12841 taatattttc acaatgggcc ttttacagga aatatttaaa gtttggtctg tttaaatctc
    12901 tttattattt ctcccactat gctgttaatg ctgttgccaa acatcatata aatccgctgt
    12961 gtttgttttt taagcgccac tttgcctctt ggagataatt cggcaaatct tataaagaaa
    13021 tattggataa tgcttttgat ttgcaaatgt atttttatat ttgatgatgt catggatgag
    13081 ttttaagtga atttcaatag aatagggaaa tgtgggtctg tgtggacaag aagtgtgatt
    13141 aatgtttttg tatcagatta taatctgcaa tctatttagt aaaattatgt ttaccaagtg
    13201 cctctcctga agaattattt ttataaagcc tttagttatc attgaaatga attctgaggt
    13261 taattggtgc gaatgaactt agtattatga taaagattgt ctttatttta cttaaaagta
    13321 tagaatagtt cattgcatta tttgtgctgt ataaaatttc agagtcagga acagtcttaa
    13381 gttttatttt ttctaaattc attggtggct atcatatggt tattagatga ctgtgataag
    13441 atatatttca ttggctttaa ggttttagta catatgacga ataggaccaa tttgagtatt
    13501 ctggaaacac caacagcctc gttgtttgtc aaaattattt tgaggttgat aatttgactc
    13561 tcgattattg taatttataa aatgtaagtg agttgaaaga tatatttgag gcatggcagt
    13621 taaatctctt atatatcgcg gagactatta ataatagtta gtctgagaca ctggaaacta
    13681 gagtgtggag agcaacgtta gttttgagta ttttctgtaa ttaatttaat ataagtttaa
    13741 ttacatatac aattatctgt tgtttgtctt aatgcaatat tttccatatg acatattttt
    13801 atatgtgttt caatacgttt attattctgc taattaaata ggagtgaaaa tgtcaaaaaa
    13861 acagattggg gttatcggta tggcggtaat ggggcgtaat cttgcactaa atatcgaaag
    13921 tcacggctat actgtttcaa tttttaaccg ttctcgtgaa aaaacagaaa aaattatagc
    13981 tactcattcg gataagaagt tatttcctta ctatacaata aaagagtttg ttgaatctct
    14041 ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat
    14101 tgatt
//
