LOCUS       AY647260               15475 bp    DNA     linear   BCT 05-JAN-2005
DEFINITION  Escherichia coli O145 O-antigen gene cluster, partial sequence.
ACCESSION   AY647260
VERSION     AY647260.1  GI:56123287
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Feng,L., Senchenkova,S.N., Tao,J., Shashkov,A.S., Liu,B.,
            Shevelev,S.D., Reeves,P.R., Xu,J., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic characterization of enterohemorrhagic
            Escherichia coli O145 O antigen and development of an O145
            serogroup-specific PCR assay
  JOURNAL   J. Bacteriol. 187 (2), 758-764 (2005)
   PUBMED   15629947
REFERENCE   2  (bases 1 to 15475)
  AUTHORS   Feng,L., Senchenkova,S.N., Tao,J., Shashkov,A.S., Liu,B.,
            Shevelev,S.D., Reeves,P.R., Xu,J., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2004) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 HongDa Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serovar="O145"
                     /db_xref="taxon:562"
     gene            118..744
                     /gene="wckD"
     CDS             118..744
                     /gene="wckD"
                     /note="role in Neu5Ac synthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="WckD"
                     /protein_id="AAV74525.1"
                     /db_xref="GI:56123288"
                     /translation="MNMKQKLIIIGAGGFAKTVIDSLDHEKYEIEGFIDTFKTGEHQG
                     YPILGDTLGVIDEPNQYLYFIAIGDPDYRALWMKLIEEMKLSTINVIDRTSIISEHSR
                     LGTCIYIGKMAIINCDSELEDGVVINTRALVEHGNYISYCTNISTNVVLNGDVFVGEK
                     SFIGSCTVVNGQLKIGNSSIIGSGSVVIRDVPDNVVVAGAPTKFIRAR"
     gene            753..1793
                     /gene="nanB"
     CDS             753..1793
                     /gene="nanB"
                     /note="Neu5Ac condensing enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaB"
                     /protein_id="AAV74526.1"
                     /db_xref="GI:56123289"
                     /translation="MSRIYIVAEIGCNHNGDFELAKKMVKEAKSAGVDAVKFQTFKAE
                     QLISKYAPKAEYQIKVTGNEETQLEMTRKLELPYDEFIKLEEYAKELGLDVFSTPFDF
                     DSIDFLASRNQKVWKIPSGELLNLPYLEKIAKLPIEDKKIVLSTGMATVDEINLALKV
                     FIDNGITHRNITILHCNTEYPTPFEDVNLNTISGFKKIFSQYNIGFSDHSPGYFAGIA
                     SVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEISLGSYEKLVTNSE
                     RKNKVVARKSIVAKCAIKKGDIFTTENITTKRPGNGISPMYWYEVLGKIAEKDFDEDQ
                     LIEHSDFSKQEV"
     gene            1797..3062
                     /gene="nnaC"
     CDS             1797..3062
                     /gene="nnaC"
                     /note="CMP-Neu5Ac synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaC"
                     /protein_id="AAV74527.1"
                     /db_xref="GI:56123290"
                     /translation="MSLKKIAIIPARSGSKGLPNKNILMLLDRPLIAYTIEAAISSNI
                     FDKIIVSTDSLEYKYIAEKYGAEVILRTKELSLDSATSFMVVQDVLEKCPGYDYFVLL
                     QPTSPFRNYKHIKNAVEQFENNHEAKFLVSVVESDKSSALIKPIDNSLSLRNFDCDFS
                     TYRRQNKKEYCPNGAIFIGYVSNYLRQKHFFGADSIAYIMNKEDSIDIDDQLDFELAI
                     LIQTKKNKKNLLDNAIIKRIVDKKDLFNKVEQITLIGHSIFDYWDLSNICGIKVNNLG
                     IAGIDSEKYYKYIIEKNMLTNIGKYVLLISGTNDIVNDGWTIEYTIKWTKNLINRVKI
                     INPDVTIILLAVPPVRGRVDRDNNTINKLNLAMKQYFSKLDNVIWMPLSPSFYDEFGN
                     LNENYTYDGLHFTLQAYKQLENDISSILK"
     gene            3059..4237
                     /gene="nnaA"
     CDS             3059..4237
                     /gene="nnaA"
                     /note="GlcNAc-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaA"
                     /protein_id="AAV74528.1"
                     /db_xref="GI:56123291"
                     /translation="MKRKLLYVTGSRAEYGIMKRLLKSLKDDPDIDLSIIATGMHCDS
                     EYGYTYKTIENDGFLIEKLIDLQLKNKTNADVLKTMSICQQAFGEHFQEKKYDAVIIL
                     GDRYEIFSVAVAASMHNLPIIHLHGGEKTLGNYDEFIRHSITKMSRLHLVSTDEYRKR
                     VIQLGEDPNCVFNVGALGAENSLQLELPSKKELENKYGGLNRKYFVVVFHPETLSTLP
                     VREQYLELLEALASFSEEYDYIFIGSNADTGSEQIREMTLDFCRVYGCRYMISVRPEE
                     YLALIKYSYGLIGNSSSGLIEVPSLKVPTINIGDRQKGRVRGDSVIDTICKKESIECA
                     IAYSQQKSFIERVKNAHNPYYNNDVMNSIVKIIKDFLTYEKLGYKDFYDVAYDYSHLK
                     "
     gene            4369..5607
                     /gene="wzx"
     CDS             4369..5607
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAV74529.1"
                     /db_xref="GI:56123292"
                     /translation="MFNTMLKYYSSVGLRGITLLTKFIFIVLLARLLPSTDLGVYGLI
                     NAAVGYGIFVVGFEFYTYSTREIINSQKNRLFFILKNQALFTVISYILCIPAFIFLLY
                     LEILPSGSEYWFILLLFFEHLSQEINRVLITIESQSIASFILFVRQGVWCWLAIAVML
                     VYPNLRNITVVFIFWFGGTVSASVLGVAYILNKKKQSDITNWDWTWIKKGIKLSVPML
                     IAALALRGFFTFDRFAVEKISGLEVLGGYTLFVSMTSAIQSFLDTILISFSFPKLALL
                     YSGKKYIKFKSELRKFTYKLILLLSFLSICCFFTGIILVKWLDKRDYIQLFPVFILLI
                     AATYIYCISLIPHIALYAMREDRYILVSQLISFLSFLLFVFFSVYQSDIYYLLIGMIA
                     SFVLLLILKMIPLYKILKKV"
     gene            5615..6802
                     /gene="wzy"
     CDS             5615..6802
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAV74530.1"
                     /db_xref="GI:56123293"
                     /translation="MNIKKDKFINGVIFFWLIISSLYYLNAIFSGVDTLKYNEDLTQK
                     IIKYIVCLVISLSILFIYKKFNYFFVLFFFLFLSVASALFSGAVTIYATTMLIIATMI
                     SFCLIIPLFSYNMVKVNRVLLWTGVIVGTISVLELTVFYNYMVSYWAATDGIRSISSL
                     LNPTNSGAYSAIIILIALVTNIKSLFKRALFLIMPMITLISSGSRTAWLSLGMTLLLT
                     VVLRDSASIRLRKKIFTLASIGTVCGALYAIFYMGSISGIESQYRGLNTYTASIRVEN
                     FLTYLNLVDLNMLLPDFLDKNINLISDNFYLVMFNYAGLIGFFIVLLILLLLIFWNIQ
                     FKIFNELMAEDIAIWRVVFIYFLISGLSNSFINSFPVNQLFFISCGYYIYKYKLVKSS
                     IGR"
     gene            6805..8061
                     /gene="wbuW"
     CDS             6805..8061
                     /gene="wbuW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuW"
                     /protein_id="AAV74531.1"
                     /db_xref="GI:56123294"
                     /translation="MQLSMSQIIECYLHATQNEIDIDKSLSKSITKKMPPLPRTSKWA
                     GERWSSSSLQIIYAVIMFAFIFGGFLLYVILFFAKYILAKFKNSISEIKVANNKEGKF
                     YYFSFSDLEMRQTCYFFKDNDSYKLSRDELIIIKLPWVNYTPTSCDFNAINLYELTSF
                     LDVARAFLLSIFSYIYYLKPSRIKWLLHIYTAPSWFLVAMGMNNIKGNLASSEHYDRW
                     AVLTDFICRIKRKRYILIQHGSLLALKTKGYEFFSLSYKLKAVSELAIFNEIELELFL
                     EHIISQANDYNIKIHFYQQPFYVSSINNKGLSILIIGHSLCERGQLSLGSQLSTLSDN
                     IVLYYKEHPKARASEKAKKTKWNFITDDDYFPDVDIVISYPSTLAYQYKELNKIVILH
                     ELDNIDQNKIDEILMTIRKNKGVYGK"
     gene            8051..9193
                     /gene="wbuX"
     CDS             8051..9193
                     /gene="wbuX"
                     /note="amidotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuX"
                     /protein_id="AAV74532.1"
                     /db_xref="GI:56123295"
                     /translation="MENKNYQICNHCIMDTSDPHITFDDSGVCNYCANYKNNILPTWR
                     KGLFSDVSLSEMASKIKADAKNSDFDCIIGLSGGLDSSYAAYIAKEKMGLRPLLFHVD
                     AGWNTDQAVGNIEKLIEGLGLELYTEVVNWEEMKDLQLSFLKSGIPDQDLVQDASFFS
                     SLYKFARQHRIKHVITGSNFSTECCREPEEWGGYLGIDTLLFNDIHTKFGEKPLKTFP
                     LVDILVYKIYYQKILGMKVHHPLNLVPFNKKDAENELNKKFGWQPFQHKHHESRFTRF
                     YEDYWLPRRFGYEKRRAHFSSLIMTGQMSREQALERISKPEMDEHFLKQEFEYVAHKL
                     GISVENLQELFFMPKKTYKDYKNKRWLIGLGANVLRALGLEKRYFR"
     gene            9190..9804
                     /gene="wbuY"
     CDS             9190..9804
                     /gene="wbuY"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuY"
                     /protein_id="AAV74533.1"
                     /db_xref="GI:56123296"
                     /translation="MITIIDYGIGNIQAFLNVYKRLGINAGVARSVDDIFSATHLIPP
                     GVGAFDQAMTLFNNSGLRDSIEKRVYEEKIPIIGICVGMQMLATSSEEGSMPGLGWIP
                     GTVRAFSSNISSQNLPMPHMGWNNLIKRTSTPLLKDFAVEPSFYFLHSYYYECDDTGD
                     VLATANYGHNFHCIISRNNIYGIQCHPEKSHSSGSQLLKNFAEI"
     gene            9809..10600
                     /gene="wbuZ"
     CDS             9809..10600
                     /gene="wbuZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuZ"
                     /protein_id="AAV74534.1"
                     /db_xref="GI:56123297"
                     /translation="MLRPRIIPCLLIHDSGLVKTVNFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSRGLEPNYDLIKKLAAECRMPLCYGGGVTSVAQATKIISLGVEKVSI
                     SSAAVENPNLVRELAEAVGKQSVVVVLDIIKRKGLFSKGYELSTRNNTRKHKIDPVSF
                     AKEMADLGAGEIVINFVDNDGVMGGYDVAYCSTIKSQINIPVTFLGGAGSYDHLSALI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTPEQKDIICNGVMSNRL"
     gene            10607..11623
                     /gene="fnlA"
     CDS             10607..11623
                     /gene="fnlA"
                     /note="4,6-dehydratase, 3-and 5-epimerizase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="AAV74535.1"
                     /db_xref="GI:56123298"
                     /translation="MMFKDKILLITGGTGSFGNAVLNRFLSTDVKEIRIFSRDEKKQD
                     DMRKNYNNKKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPVEAVKTNV
                     LGTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDCNKTVI
                     CGTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGN
                     NGDIFVQKAPAATIETLATALKDLLNAAHHPVNIIGTRHGEKLFEALLSREEMLSAED
                     MGDYYRIPPDLRDLNYGKYVEQGDSRISEVEDYNSHNTQRLNVEGMKTLLLKLDFIRA
                     LHAG"
     gene            11628..12749
                     /gene="fnlB"
     CDS             11628..12749
                     /gene="fnlB"
                     /note="reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="AAV74536.1"
                     /db_xref="GI:56123299"
                     /translation="MAKTHDIKILITGANGFIGRNLSLRLEELGYKNLIRIDRDSTEQ
                     DLEQGLQDADFIYHLAGVNRPKTDDEFKSGNSDLTKYIVEYLISIGKKIPIMLSSSIQ
                     AELDNAYGVSKALAERYIEKYATTSGSPYYIFRYPNVFGKWCKPNYNSFVATFCYNIA
                     NDLAITINDASAPVNLVYIDDVCAKAIELLSGATESGYKAVAPIYSTTVGEVAELIYR
                     FKNSRSTLITEAVGTGFTRALYSTWLTYLPAEKFAYKVPFYGDARGVFCEMLKTPSAG
                     QFSFFTAHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELNVSSDEFKIVE
                     TVPGWTHDITNIGTEELIVMLWANEIFNRDEPDTIARPL"
     gene            12749..13879
                     /gene="fnlC"
     CDS             12749..13879
                     /gene="fnlC"
                     /note="C-2 epimerizase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="AAV74537.1"
                     /db_xref="GI:56123300"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHIIVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRHKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMFEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            13879..15090
                     /gene="wbuB"
     CDS             13879..15090
                     /gene="wbuB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuB"
                     /protein_id="AAV74538.1"
                     /db_xref="GI:56123301"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRVVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGYLVKKIKARCQCPAYLILRDMFPQWVIDAGILNAGSPIERYFRLFEKI
                     SCRQANRIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAAYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGSGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            15077..15475
                     /gene="wbuC"
     CDS             15077..15475
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="AAV74539.1"
                     /db_xref="GI:56123302"
                     /translation="MRLIDNDQLDKLYDQAEQSERLRSHLIMHGSHQEKVQRLLIALV
                     KGSYVEPHYHKLPHQWEMFIVMEGQLQVCLYGRNGEVIKQFIAGDNIGISIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDPSFAKSFV"
BASE COUNT     5033 a   2292 c   3025 g   5125 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgtgtttttg agatatttat gtattatcgt
       61 tgtcatgaat acaagtaaca acaaagttaa tgtgatctaa tcaaaccatt agatggtatg
      121 aatatgaagc aaaaattaat aattattggt gctggtggtt ttgctaaaac tgtaattgac
      181 agtttggatc atgaaaagta tgaaatagaa gggttcattg atacatttaa aacaggtgag
      241 catcaaggat accctatttt aggtgatact ttaggtgtca tagacgaacc taaccaatac
      301 ctatacttta ttgcaattgg agatcccgat tacagagcct tatggatgaa gttaatagaa
      361 gaaatgaaac tttcaactat caatgtaatt gacagaacat ctataatttc tgaacattct
      421 aggcttggta cctgcattta tatagggaaa atggctatca ttaactgtga ttcagaacta
      481 gaagatggag tggtgataaa cacaagagca ttagtagagc atgggaatta tatatcatat
      541 tgtacaaata tctctactaa tgttgttctt aatggtgatg tattcgttgg ggaaaaatcc
      601 tttataggaa gttgtaccgt tgttaatggg caactaaaga taggtaattc atcaattatt
      661 ggttctggtt ctgtagttat tcgtgatgtt cctgacaatg tagtagtggc cggggctcct
      721 acaaagttta ttagagcaag gtgattttta ttatgtcgag aatttatatt gttgctgaaa
      781 ttggttgtaa tcataacgga gatttcgagt tagctaagaa aatggtaaaa gaggcaaaat
      841 ctgccggggt agatgcagta aaattccaaa cttttaaagc tgagcaactt atctcaaaat
      901 atgctcctaa agcagaatat caaataaaag ttaccggtaa tgaagagact caattagaga
      961 tgactcgtaa gttagagttg ccatatgatg agtttatcaa gctggaagaa tatgcaaaag
     1021 aacttgggtt ggatgtcttc tcaacaccat ttgattttga ttcaatcgat tttcttgcat
     1081 ccagaaatca aaaagtttgg aagattccat caggcgaatt attaaattta ccatatttag
     1141 agaaaatagc caaattgcca atcgaagata aaaaaattgt actatcaact ggtatggcaa
     1201 cagttgatga aatcaatttg gcattaaagg tttttattga taatggcatt acacatagaa
     1261 atataacaat attacattgt aatacagagt atccaacgcc atttgaagat gtaaatctaa
     1321 atacaatttc tggattcaaa aaaatattta gtcaatataa tattggattt tctgatcatt
     1381 ctccaggtta ttttgctggt attgcatcag tgccttatgg gatcactttt attgagaaac
     1441 attttacact ggataaaaat tttgaaggtc cagatcataa agcatcagtt acacctgagg
     1501 aacttaaatt actatgcgaa ggaatacgtg cagtagaaat ttcacttggt agctatgaaa
     1561 agttagtaac aaattcagaa cgaaagaata aagtagtagc tcgaaaatca attgttgcta
     1621 aatgtgcaat aaaaaaagga gatattttta cgacggaaaa tattacgaca aagcgaccag
     1681 gaaacggaat tagtccaatg tactggtatg aggtcttagg taaaatagct gaaaaggatt
     1741 ttgatgagga tcaactaatt gagcactctg attttagtaa acaagaggtt tgagttgtgt
     1801 cattaaaaaa aattgccatt atacctgctc ggtctggatc taagggctta ccaaataaaa
     1861 atatactaat gttattggat cgcccattaa ttgcttatac tatcgaagca gcgattagct
     1921 ctaatatatt tgataaaatt attgtttcaa cagattcatt agaatataaa tatattgcag
     1981 agaaatatgg cgcggaagta atattacgca caaaagaatt atcgttggat tcggcaacat
     2041 cttttatggt agttcaagat gtgcttgaaa aatgtccagg atatgattat tttgtgcttc
     2101 tacaaccaac atcaccattt cgtaattata agcatattaa gaatgctgtt gagcaatttg
     2161 aaaataatca cgaagcaaaa tttttggtgt ctgtggtaga aagtgataaa agttcggcgt
     2221 taattaaacc aattgataat tcactgtcat taaggaactt tgactgtgat tttagtacat
     2281 atcgtagaca aaataaaaaa gaatactgtc caaatggtgc gatttttatt ggttatgttt
     2341 ctaattattt aagacaaaaa catttctttg gtgctgacag tatagcctat attatgaata
     2401 aagaagattc tattgatatc gatgatcagt tagattttga attggcaatt ctaatccaaa
     2461 caaaaaaaaa taaaaaaaat ttattggata atgctattat caaacgaatt gtcgataaaa
     2521 aagatttatt taacaaagtt gagcaaatta cactgattgg acattcaatt tttgattatt
     2581 gggatctcag caacatatgt ggaattaagg ttaacaattt aggcattgct ggcattgata
     2641 gcgaaaaata ttacaaatac atcattgaaa aaaacatgct caccaatata ggtaagtatg
     2701 tgctattaat ttcaggcaca aacgacattg tgaacgatgg ttggactatt gaatacacta
     2761 taaaatggac aaaaaatctg atcaatagag taaaaattat aaatcctgat gttactatca
     2821 ttctgctagc agtgccgcct gttcgaggaa gagttgatag ggataataat actatcaata
     2881 aattaaatct ggcaatgaaa caatatttta gtaaattaga caatgttatt tggatgccgt
     2941 tatctccatc tttttatgat gagttcggca atctgaatga aaattatact tatgatggtt
     3001 tacatttcac gttacaagct tacaaacagt tagaaaatga tatttcgagt atcttaaaat
     3061 gaaaagaaaa ttgctctatg tcaccggctc aagagctgag tacggtatta tgaaacgact
     3121 attaaaaagt ctaaaagatg atcctgatat tgatttatca attattgcaa caggtatgca
     3181 ttgcgattct gaatatggtt atacatataa aacaatcgag aatgatggtt tcttaattga
     3241 aaaattaatt gatttgcagc tgaaaaataa aaccaacgca gatgttttga aaacgatgtc
     3301 gatatgccaa caagcatttg gtgagcattt ccaagagaaa aaatatgatg ctgtcattat
     3361 attaggtgat cgatacgaaa tattttcagt ggcagtagca gcttctatgc ataacctacc
     3421 tattattcat cttcatggtg gagagaaaac gttagggaat tatgatgaat ttatacgaca
     3481 ttcaataacg aaaatgagtc gtctacatct agtatcaact gatgaatatc ggaaaagggt
     3541 tatccaactt ggtgaagatc ctaattgtgt atttaatgta ggtgcgctag gggctgaaaa
     3601 cagtttgcaa ctagaattac cctctaaaaa ggagcttgaa aataagtacg gtgggttaaa
     3661 tagaaaatat ttcgtagtcg tatttcatcc tgaaacgtta tcaactttac ctgttagaga
     3721 gcagtattta gaattactag aagcattagc tagttttagc gaagaatacg attatatctt
     3781 tattggctca aatgctgata ctggttcaga acaaataaga gaaatgactc tcgatttttg
     3841 tagagtatat ggctgtcgat atatgatttc tgtgagacct gaagaatatc tagctttgat
     3901 aaaatattca tatggcttaa taggcaattc gtcatctggg ttaattgagg ttccaagctt
     3961 aaaggttccg acgattaata taggcgatcg ccaaaaagga agggttagag gtgattcggt
     4021 aatagatacc atatgtaaaa aagaaagtat agaatgtgcc attgcttatt cacaacagaa
     4081 aagtttcatt gaacgtgtaa aaaatgctca taatccttac tataataatg atgtaatgaa
     4141 tagtattgtt aaaatcatta aagatttttt aacttatgaa aagttgggtt ataaagactt
     4201 ctatgatgtt gcatatgact atagtcattt aaaataaaca acatgggtat tgtttttaat
     4261 aagttatttg tatgatattt taaatttaaa aacctcatat taatatttaa attattgcaa
     4321 atttaataat cacagattaa aaacttccga ttttaacatt ggccttggat gtttaatact
     4381 atgcttaagt attactcaag tgttggatta agagggatta ctctacttac taaatttatt
     4441 ttcattgttt tgcttgctcg acttttacca tcaacagatt taggagtgta tggattaatt
     4501 aatgcagctg taggatatgg tattttcgtt gtaggttttg agttttatac gtattcaacg
     4561 agagaaataa ttaactcgca aaaaaatagg ctttttttta tactaaaaaa tcaagctcta
     4621 tttactgtta tatcttatat actatgtata ccggcattta tttttttatt atatttagaa
     4681 atattaccat ctggaagtga atactggttt atcctacttt tattttttga gcacttatca
     4741 caagagatta atagagttct aataacaata gaaagtcaat cgattgcaag ttttattctt
     4801 tttgtaagac aaggtgtatg gtgttggtta gctatagctg tgatgctagt gtatccgaac
     4861 ttaagaaata taacagttgt atttattttt tggtttggtg gtactgtgtc cgcgagtgtg
     4921 cttggagtgg cttatatttt aaataaaaaa aaacaaagcg atattacaaa ctgggattgg
     4981 acgtggataa aaaaaggtat aaagctgtct gtaccaatgc taattgcagc ccttgcacta
     5041 cgaggctttt tcacgtttga tagattcgcg gtagaaaaaa tatcgggcct agaagttttg
     5101 ggaggatata cattatttgt tagtatgact tcagctattc aatcattttt ggatactatt
     5161 ttgatatctt tttcatttcc aaagcttgcc ttgttatatt cagggaaaaa atatataaaa
     5221 tttaaatctg agttaagaaa attcacttat aaattaattt tactactatc tttcttgagc
     5281 atctgttgct tttttactgg gattatattg gttaagtggt tggataaacg agattacata
     5341 caattatttc ctgtatttat attattaata gcagcgactt atatctattg tataagtctt
     5401 attccacata ttgctttata cgcgatgaga gaagatcgtt acatattagt aagtcaactg
     5461 atatcatttt tatctttttt actatttgtt ttttttagcg tatatcaaag tgatatctat
     5521 tacttgctaa ttggtatgat agctagtttt gtattacttt tgatcttaaa aatgatcccg
     5581 ttatataaaa ttctaaaaaa ggtttaaaat aaacgtgaat ataaagaaag ataagtttat
     5641 aaatggagtg attttttttt ggttaattat ttcttcgtta tattacttaa atgctatttt
     5701 ttctggtgtt gacacattaa aatataatga agatttaacg caaaaaatta taaaatatat
     5761 agtttgctta gttataagtc taagtatctt atttatttac aagaaattta attatttttt
     5821 tgtattgttt tttttcttgt tcctgtctgt tgcttcagcc cttttcagtg gtgcggtaac
     5881 aatttacgca acaacaatgt tgattattgc aactatgatc agcttttgcc tgattattcc
     5941 tctattttct tataatatgg tgaaagttaa tagagttctt ttatggacag gagttattgt
     6001 aggcacgatt tctgtattag aattaacggt attttataat tatatggttt catattgggc
     6061 tgccactgat gggattaggt caatatcttc tcttctgaat cctacgaata gtggtgctta
     6121 ttcagcgatt attattttaa tcgccttggt gacaaatata aaaagtcttt ttaaaagagc
     6181 tttatttctt ataatgccga tgataacgtt aattagcagt ggttcgcgca cagcatggtt
     6241 atcacttggt atgacacttt tattaacagt agtattgaga gacagtgcca gcattcgctt
     6301 gcgaaaaaaa atatttactc ttgcaagcat tggcactgtt tgcggtgcat tgtacgccat
     6361 attttatatg ggcagtatct ctggtattga atcacaatat cgaggtctta atacgtatac
     6421 tgcatcaatt cgagttgaaa actttctgac atatttaaat ttagttgatc tgaatatgtt
     6481 gctacctgat tttttagata aaaatataaa tctcatttca gataactttt atctcgtaat
     6541 gtttaattat gccggtctaa tcggcttttt tattgtttta ttaattttat tgctgcttat
     6601 cttctggaac atacaattta aaatatttaa tgagttaatg gctgaagata tagccatttg
     6661 gagagttgtt tttatttatt tcctaatatc cgggctttca aattcattta taaattcttt
     6721 tcctgtaaat caattgttct ttatctcatg cggatattat atatataaat ataaattagt
     6781 taaaagctct ataggaagat aaatatgcaa ttatcaatgt cgcaaataat agaatgttat
     6841 ttacacgcaa cacaaaatga aatagatatc gataagtctt tatcaaaaag cattacaaaa
     6901 aaaatgccac cacttcccag gacaagtaaa tgggctgggg aaaggtggag ttcatcgagt
     6961 ttgcaaatta tttatgcagt gattatgttt gcctttattt ttggggggtt tttattatat
     7021 gttatattat tttttgctaa atatatattg gctaaattta aaaattccat ttcagaaata
     7081 aaggttgcta ataataagga aggtaaattt tactattttt cgttttcaga tttagaaatg
     7141 aggcaaactt gctatttttt taaagataat gatagttaca aattatctcg agatgaactt
     7201 attataataa aattaccatg ggtaaattat acaccaacga gttgtgactt taatgctatt
     7261 aatttatatg aattaacatc gtttctagat gtggcaagag cattcttgtt atctattttt
     7321 agttatatct attatttaaa accatcacgt ataaaatggc ttcttcatat ttatacagca
     7381 ccttcttggt ttttggttgc tatgggaatg aataatatta aaggcaatct cgcaagtagt
     7441 gaacattatg atcgttgggc tgttttaact gattttatat gcagaataaa aaggaaaaga
     7501 tatatattaa ttcaacatgg ctcgttactg gcgttaaaga ctaagggtta tgaatttttc
     7561 tctctctcat ataaactgaa agctgtatca gagctcgcta tctttaacga aattgagctt
     7621 gaattatttt tagagcatat aatctcacag gctaatgatt acaatataaa aattcatttc
     7681 tatcaacagc cattttatgt ttcatcaatt aataataaag gattatcaat tctaattatt
     7741 gggcattcat tgtgtgaacg aggacaacta agtcttgggt cacaactttc aacgttatca
     7801 gataatattg ttctttatta taaagaacat ccaaaagcta gagcatctga aaaagccaaa
     7861 aagacaaaat ggaatttcat tacagatgat gattatttcc ctgatgtaga tattgtcatt
     7921 tcgtatcctt ccactttggc atatcaatac aaagagttga ataaaatagt tattcttcat
     7981 gaattagata atattgatca aaataaaatt gacgagatac ttatgaccat tagaaaaaat
     8041 aagggcgtgt atggaaaata aaaattatca aatttgtaat cattgtatta tggatacatc
     8101 tgatcctcat attacttttg atgatagcgg agtatgcaat tactgcgcca attataaaaa
     8161 caatatattg ccgacatgga gaaaaggttt gttttcagat gtttctttat ctgaaatggc
     8221 aagtaaaata aaggcggacg caaagaatag tgattttgat tgtattattg gactatcagg
     8281 ggggcttgat agttcatatg ctgcatatat tgcaaaagaa aagatggggc tacgcccatt
     8341 attattccat gttgatgccg gctggaatac agatcaggct gttggtaata ttgaaaaact
     8401 tattgaaggc cttggtttag aactttatac agaggtagta aactgggagg aaatgaaaga
     8461 tcttcaactg tctttcttaa aatcaggaat tccagatcaa gatctagttc aggacgcttc
     8521 ctttttttca tcattatata agtttgctcg acaacataga attaaacatg tcattacggg
     8581 gtccaatttt tctactgagt gttgtcgtga accagaagaa tggggaggat atttaggcat
     8641 tgatactctg ttatttaatg atatccatac gaaatttgga gaaaaaccgt taaaaacttt
     8701 tcctttagtg gatatattgg tttataaaat ttactatcag aaaattcttg gtatgaaagt
     8761 gcatcatcca ctaaatttag ttccttttaa taagaaggat gcagaaaatg aacttaataa
     8821 aaaatttggc tggcaaccct tccagcataa acatcatgag tcccgtttca cccggtttta
     8881 tgaagattat tggctgccac gcagatttgg ttatgagaaa cgaagagctc acttttcgag
     8941 tttaattatg acgggacaaa tgtctcgtga acaagctctt gagaggattt caaaaccaga
     9001 aatggatgag cactttctta aacaagagtt tgagtatgta gcgcacaaac ttggtattag
     9061 tgttgaaaat ttgcaagagc tattttttat gcccaaaaaa acatataagg attataagaa
     9121 taagcgttgg ttgattggtt taggtgcgaa tgtcttacgt gcacttggat tagaaaaaag
     9181 gtatttcaga tgattacaat cattgattat ggtattggaa atatacaagc atttttgaat
     9241 gtatataagc gactaggcat taatgcgggt gttgcccgtt ctgttgatga tatttttagt
     9301 gctacacatt taataccgcc tggagttggg gcttttgatc aagctatgac tctttttaat
     9361 aattcaggtt tacgagatag tattgaaaaa cgtgtatatg aagaaaagat tccaattatt
     9421 ggtatctgtg tgggtatgca aatgcttgcg acctctagtg aggaggggag catgccaggt
     9481 ttaggatgga ttccaggtac ggttcgagca tttagtagca atatctcttc acaaaacctc
     9541 ccgatgcccc acatgggatg gaataattta attaaaagaa caagtacacc attgctgaaa
     9601 gatttcgcag tagaaccttc attttatttt ttacactcat actattacga atgtgatgac
     9661 acaggtgatg tattggcaac tgctaattat ggtcataatt ttcattgtat aattagcagg
     9721 aataacatat atggtattca atgtcatcca gagaaaagcc attcatcggg atcccaacta
     9781 ctaaaaaatt ttgcagagat ctaatagcat gcttagacca agaatcatac cctgtttact
     9841 tatccacgat tctggcttag tcaaaacagt taattttaaa tcccctaagt atgttggtga
     9901 tccaattaat gccgtcaaaa ttttcaatga gaaagaagct gatgaattga tggtcctaga
     9961 tattgatgcg acatccaggg gactcgaacc taattatgat ttaataaaaa aactcgcagc
    10021 ggaatgccgt atgcctttat gctatggagg aggcgtaacc agcgtagcac aggctacaaa
    10081 aattatctct cttggtgttg aaaaggtatc aataagttca gcagctgttg aaaatcctaa
    10141 tcttgttaga gaattggcag aagcggttgg aaaacaaagt gttgttgttg tgctggatat
    10201 tatcaaacga aaaggtctat tctcaaaggg ctatgaactt tcgacaagga ataacactcg
    10261 caagcataaa atcgatcccg tctcgtttgc aaaggaaatg gccgatctgg gagctggaga
    10321 aattgtcatc aattttgtag ataatgatgg tgtcatggga ggatatgacg ttgcatattg
    10381 ctcaactatc aaatcgcaaa taaatattcc tgttacgttt ctcgggggcg ctggtagtta
    10441 tgaccatctt tctgcactga ttgaccaatg tggcatcgtt ggtgcggcag ctggtagtct
    10501 ttttgtattt aaagggaagt atagggcagt attgataagc tatccaacac ctgaacagaa
    10561 agatattatt tgtaatggtg taatgagtaa tcgtttataa ggggttatga tgttcaaaga
    10621 taaaatactg ttaatcactg gtggtactgg ttcatttggg aatgctgtat taaatcgctt
    10681 tctttccaca gatgtaaaag aaatacgcat atttagtcgt gatgaaaaaa agcaagatga
    10741 tatgcgtaaa aattataaca ataaaaaatt gaaattctat attggtgatg ttagagatta
    10801 tcgtagtatt ttgaatgcga ctcgcggagt tgattttata tatcacgcag cggcacttaa
    10861 gcaagttcca tcatgtgagt ttcatcctgt ggaggctgtt aaaactaatg ttcttggtac
    10921 agaaaatgtg cttgaagctg ctatagcgaa tgaagttaaa agggttgtgt gtcttagtac
    10981 tgataaagct gtatatccga tcaacgctat gggaatttca aaagctatga tggaaaaagt
    11041 catggttgcg aaatcgcgta atgttgactg caacaaaaca gtaatatgtg gtacccgtta
    11101 tgggaatgtc atggcctctc gtggatctgt tattccatta tttgttgatc ttatcaaatc
    11161 tggcaagcca ctaacaatta ctgaccctaa catgacccgt tttatgatga ctcttgagga
    11221 tgctgtagat ttagtacttt atgcattcga acatggtaat aacggagata tttttgtaca
    11281 aaaagctcca gctgcaacta tcgaaacatt agcaactgct ttaaaggatt tactaaatgc
    11341 tgctcatcac ccagtaaata ttattggtac gcgtcatggt gagaagttat ttgaagcttt
    11401 gctcagtcgt gaggaaatgc tctctgctga agatatgggg gattattatc gtataccacc
    11461 agatcttcgt gaccttaatt atggaaaata tgttgaacaa ggtgatagtc gtatatccga
    11521 ggttgaagat tacaactctc ataatacaca acggttgaac gttgaaggca tgaaaacgct
    11581 tttgctcaaa ttggatttta ttcgtgcact tcatgcaggt taacaacatg gctaagactc
    11641 atgatataaa aatattgatt actggtgcaa atggttttat tggccgtaat ttaagtttga
    11701 ggcttgagga actaggttac aagaatctta ttagaattga tcgggattca acagaacaag
    11761 atcttgaaca aggcttacag gacgctgatt tcatttatca tttggctgga gtcaatagac
    11821 caaagactga tgatgaattt aaatccggaa acagtgattt aactaaatac atagttgagt
    11881 atcttatttc tataggaaag aagataccaa ttatgctaag ttcttctata caagcagaac
    11941 ttgacaatgc ttatggggta agcaaagctt tagctgaacg ttatattgaa aaatatgcta
    12001 ctactagtgg ttctccatat tatattttca gatatccaaa cgtttttggc aaatggtgca
    12061 aaccaaatta taattctttt gttgcgactt tttgctataa tattgcgaat gatcttgcca
    12121 taactatcaa tgatgcatct gcgccagtta atttggttta tattgatgat gtttgtgcta
    12181 aagcgataga gcttctctcg ggggcaactg aaagcggata taaagctgtt gcaccaattt
    12241 attcaactac tgttggtgaa gttgcagaat taatttatag attcaagaat agccgttcca
    12301 ccctgatcac agaggctgtc gggacgggat ttacccgtgc attgtattct acatggctga
    12361 cttatttacc agcagagaag tttgcgtaca aggtaccttt ttatggggat gcccgcggag
    12421 tcttttgtga gatgttgaaa acgccttcag cggggcagtt ttcatttttt actgctcacc
    12481 ctggtattac gcgtggcgga cattaccatc acagtaaaaa tgagaagttt ttggtcattc
    12541 gaggtcaggc atgctttaaa tttgaacatg tgattaccgg tgagcgatat gaactgaatg
    12601 tttcatcgga tgagttcaag attgttgaaa cagttcctgg ttggacacat gacattacaa
    12661 atattggaac tgaggaatta atagtcatgc tctgggcaaa tgaaattttc aaccgtgatg
    12721 agcccgatac tattgcgaga cctctataat gaaaaaatta aaagttatgt ctgttgttgg
    12781 aacccgtcct gagattatcc gtttgtcgag ggttcttgct aagtttgatg aatactgcga
    12841 gcatattatt gtccatactg gtcaaaatta tgattacgaa ttaaatgaag tattcttcaa
    12901 tgacttgggt gttcgaaaac ctgattattt tttaaatgca gcgggaaaaa atgcggcgga
    12961 aaccattggc caagttatta ttaaggtaga tgaagtatta gaaatcgaaa aacctgaagc
    13021 aatactggta ttgggcgata cgaattcatg tatttctgcc attccggcca aacgccataa
    13081 agttcctata tttcatatgg aagcaggtaa ccgttgtttc gatcaacgcg tgcctgaaga
    13141 aaccaacaga cgtattgttg accatacggc tgatatcaat atgacctaca gtgatattgc
    13201 tcgtgaatat ctcttggctg aaggtatccc agctgatcgg atcataaaaa ctggtagccc
    13261 tatgtttgag gttctttcat attatatgcc ccaaattgat ggttcagatg tgctatcgcg
    13321 tttgaatcta cagtctggtg agttttttgt agtaagtgcg catcgtgaag agaatgttga
    13381 ttctccaaaa cagctcgtaa agcttgcgaa cattctaaat actgttgctg aaaaatataa
    13441 tcttccagtt attgtctcca cacacccaag gacacgtaac cgaatccgtg agcaaggaat
    13501 tgaatttcat tcaaatataa atctactgaa accattgggt ttccatgatt ataaccactt
    13561 gcagaagaac tcacgagctg tgctttcaga tagcggtact atcactgaag agtcatccat
    13621 catgaatttc ccagcggtaa acatccggga agcgcatgag cgtccggaag gctttgagga
    13681 agcatccgtc atgatggtgg ggttagagtg tgaacgcgta ttacaagcgc tggatattct
    13741 ggcaacacaa ccgcgaggtg aagtccgtct tttacgtcag gttagtgatt acagcatgcc
    13801 aaatgtgtcg gataaagttg tcagaattgt tcactcttac acagattatg ttaagagagt
    13861 tgtctggaaa gaatattgat gaaacttgct ttaatcatag atgattacct gcccaacagt
    13921 actcgtgttg gtgcaaaaat gtttcatgaa cttgctcaag aatttatcca gcgtgggcac
    13981 gatgttacgg taattactcc tggtacgggc atgcaagaag agatttcttt tgataccttt
    14041 cagggggtaa aaacatggcg ttttaaaagc gggccgctca aggatgtaag taaaattcag
    14101 cgagtggtca atgaaacgct tttgtcctat cgggcgtgga aagccatcaa aaaatgggta
    14161 aaaaaagaga cctttgaggg tgtgatttat tattcacctt ccatattctg ggggtattta
    14221 gttaaaaaaa ttaaagctcg ttgccaatgt cctgcttatc ttattttaag agatatgttt
    14281 ccacaatggg taattgatgc aggaatcctt aatgctggtt ccccaataga acgctacttt
    14341 cgtctttttg aaaaaatatc ttgtcgtcag gcaaatcgta ttggacttat gtctgataag
    14401 aatcttgatg tttttcggaa agataataaa ggctatccgt gcgaagtttt gcgtaattgg
    14461 gcatccctaa caccaacgat catacccaag gattacatac cactacgtaa gcgacttggc
    14521 ctagaggata aaaccatttt cttctatggt ggaaacatag gtcatgcaca ggacatgaca
    14581 aacttgatgc gacttgtgag aaacatggca gcatatcctc aagctcattt cctatttatt
    14641 ggccagggtg atgaagttga attaattaat tcattagcct ctgagtgggc attgacgaat
    14701 ttcacctatt tgccctcggt taatcaggat gaatttaagt tcattttgtc ggaaatggat
    14761 atcggcttgt tttctctttc cgctagacac tcttcccata attttcctgg taagttatta
    14821 ggctatatgg ttcagtcgct acctatttta ggtagcgtaa atgccggaaa tgatttgctc
    14881 gacattgtca atcaaaataa tgccggatta atccatgtca atggtgagga cgataaatta
    14941 tgtcaatctg cgctattaat gttgcatgat attgatgtgc gccggcaact tggttcgggg
    15001 gcgaatatat tgttgaaaga acaattctcc gttgagtctg cggcacagac gatagaaatg
    15061 aggttggagg catgcaatgc gattaattga taatgaccaa ctcgacaaat tatatgatca
    15121 agccgagcaa tcggaacgtt tacgttccca ccttattatg cacggctcgc atcaagaaaa
    15181 ggtacagcgt ttacttattg cattagtaaa gggcagctat gttgaaccgc attatcacaa
    15241 acttcctcat cagtgggaaa tgttcattgt tatggagggg caacttcagg tttgtttgta
    15301 tggtagaaat ggtgaggtta taaagcaatt tatagcagga gataatattg gaataagcat
    15361 tgtggagttt tctccgggcg atatacacag tgtcgaatgc ctatctccgc gtgctcttat
    15421 ggtggaagtt aaagaggggc catttgaccc ttcttttgca aaatcgttcg tgtga
//
