LOCUS       DQ167407               10241 bp    DNA     linear   BCT 27-DEC-2006
DEFINITION  Escherichia coli O148 O-antigen gene cluster, complete sequence.
ACCESSION   DQ167407
VERSION     DQ167407.1  GI:76366001
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Feng,L., Perepelov,A.V., Zhao,G., Shevelev,S.D., Wang,Q.,
            Senchenkova,S.N., Shashkov,A.S., Geng,Y., Reeves,P.R., Knirel,Y.A.
            and Wang,L.
  TITLE     Structural and genetic evidence that the Escherichia coli O148 O
            antigen is the precursor of the Shigella dysenteriae type 1 O
            antigen and identification of a glucosyltransferase gene
  JOURNAL   Microbiology (Reading, Engl.) 153 (PT 1), 139-147 (2007)
   PUBMED   17185542
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Feng,L., Perepelov,A.V., Zhao,G., Shevelev,S.D., Wang,Q.,
            Senchenkova,S.N., Shashkov,A.S., Geng,Y., Reeves,P.R., Knirel,Y.A.
            and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O148"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABA42227.1"
                     /db_xref="GI:76366002"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTHDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSISGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNDTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPNHFPEKLIPLVILNALEG
                     KGLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    108..8794
                     /note="O-antigen gene cluster"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ABA42228.1"
                     /db_xref="GI:76366003"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNCDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQLGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABA42229.1"
                     /db_xref="GI:76366004"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNGVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAESL
                     KKNAYGKYLLKMIKGY"
     gene            3033..3590
                     /gene="rmlC"
     CDS             3033..3590
                     /gene="rmlC"
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ABA42230.1"
                     /db_xref="GI:76366005"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKIFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYNPSFEKSISYSDPTIKIQWPNL
                     QDMHFKLSNKDLNAKNFFNNNSLMQ"
     gene            3587..4777
                     /gene="wzx"
     CDS             3587..4777
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABA42231.1"
                     /db_xref="GI:76366006"
                     /translation="MKKNILLLFLVHGANYLFPFIVLPYQTRILSIETFADVAKIQAA
                     VMLLSLIVNYGYNLSSTRAIARAVSQAEINKIYSETLIVKLLLATICLALGYVYLMYV
                     KEYSLIYPFIISSIYLYGSALFATWLFQGLEKMKAIVIATTIAKLTGVILTFILVKSP
                     NDIDAALFTQNIGMFISGIISIYLVRKNKYATVIYFRLKNIILSLKEAWPFFLSLAAT
                     SAYTYFNVILLSFYAGDYVVANFNAADKLRMAAQGLLIPIGQAVFPRLSKLEGYEYSS
                     KLKIYAIRYAIFGVCISAGLVFLGPMLTTIYLGKEYSLSGEYLQSMFLLPAIISISTI
                     LSQWMLIPQGKEKILSRIYILGAIFHLLYAFPLVYYYGAWGMVISILFTEVLIVLFML
                     KAVK"
     gene            4774..5916
                     /gene="wzy"
     CDS             4774..5916
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABA42232.1"
                     /db_xref="GI:76366007"
                     /translation="MTYFTGFILILFAIIIKRLTPSQSKKNIVLIANAFWGILLVGYA
                     FNEQYFVPLSATTLFFILAFLFFFSMTYILIARSGRVVFSFGTGFIESKYIYWFAGMI
                     NIISICFGIILLYNNHFSLKVMREGILDGSVSGFGLGISLPLSFCCMYLARHENKKNY
                     FYCFTLFSFLLAVLSTSKIFLILFLVYIVGINSYVSKKKLLIYGTFVFGLFALSSIIL
                     GKFSSDPEGKIISAIFDTLRVYLFSGLAAFNLYVEKNVTLPENLLLYPFKEVWGTTKD
                     IPKTDILPWINTGVWDTNVYTAFAPWYQSLGLYAAIIIGILLGFYYGIWFSFRQNLAV
                     GFYQTFLCFPLLMLFFQEHYLLSWKMHFIYFLCAILLAMRKALEYE"
     gene            5909..6757
                     /gene="wbbR"
     CDS             5909..6757
                     /gene="wbbR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbbR"
                     /protein_id="ABA42233.1"
                     /db_xref="GI:76366008"
                     /translation="MNKYCILVLFNPDISVFIDNVKKILSLDVSLFVYDNSANKHAFL
                     ALSSQEQTKINYFSICENIGLSKAYNETLRHILEFNKNVKNKSINDSVLFLDQDSEVD
                     LNSINILFETISAAESNVMIVAGNPIRRDGLPYIDYPHTVNNVKFVISSYAVYRLDAF
                     SNIGLFQEDFFIDHIDSDFCSRLIKSNYQILLRKDAFFYQPIGIKPFILCGRYLFPIP
                     SQHRTYFQIRNAFLSYRRNGVTFNFLFREILNRLIMSIFSGLNEKDLFKRLHLYLKGI
                     KDGLKM"
     gene            6782..7693
                     /gene="wbbQ"
     CDS             6782..7693
                     /gene="wbbQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbbQ"
                     /protein_id="ABA42234.1"
                     /db_xref="GI:76366009"
                     /translation="MIQKKVAAIIITYNPDLTILRESYTSLYKQVDKIILIDNNSTNY
                     QELKKLFEKKEKIKIVPLSDNIGLAAAQNLGLNLAIKNNYTYAILFDQDSVLQDNGIN
                     SFFFEFEKLVSEEKLNIVAIGPSFFDEKTGRRFRPTKFIGPFLYPFRKITTKNPLTEV
                     DFLIASGCFIKLECIKSAGMMTESLFIDYIDVEWSYRMRSYGYKLYIHNDIHMSHLVG
                     ESRVNLGLKTISLHGPLRRYYLFRNYISILKVSYIPLGYKIREGFFNIGRFLVSMIIT
                     KNRKTLILYTIKAIKDGINNEMGKYKG"
     gene            7703..8794
                     /gene="wbbG"
     CDS             7703..8794
                     /gene="wbbG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbbG"
                     /protein_id="ABA42235.1"
                     /db_xref="GI:76366010"
                     /translation="MKKIIHIQVLPKMSGVQQISFDILSGLKDKDVQKFILCGLPDGC
                     SDNEFKKKFTDIGVRVITIPTLKRNIGWHDFRCFIDLYNFFKKEKFDIVHTNSTKPGI
                     IARIAARLAGTKLIIHTVHGIAFHRKENTVRKIFYYCLENFATLFGSINVTVNENYLK
                     YYPFVKSHIIYNGVDFNVLCCNKKDHDFLHIAFMARLDKQKNPLEFIRAVNIIKKKLP
                     NERLKFTLAGCGELENECKKLIEHFHLTDVIDMPGWIVDKNTFYNSVDIICQPSNWEA
                     FGLVFVEAAFFEIPSVSRNIEGIPEVILDNETGLLYEGGEAELSEKLISLIHDKKKIS
                     WLGLNAKEYVLKHFTKDIMVEKYSKLYDL"
BASE COUNT     3106 a   1709 c   2151 g   3275 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagtat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 atgatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgcag
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgtgat gcagctgcaa
      301 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggca gctgaaagcc
      361 atgttgaccg ttcaatttca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt agaagcggct cggaattact ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct catccagatg
      541 aagtaaatga tacagaagaa ttacccttat ttactgaaac aacagcttac gcaccaagca
      601 gcccttattc tgcatcaaaa gcatctagcg atcatttagt ccgtgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgct ctaacaatta tggtcctaat catttcccgg
      721 aaaaattgat tccattggtt attttgaatg ctctggaagg taaaggatta cctatttatg
      781 gaaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgtc ccgaaagaga
      961 aatcttaccg cgagcaaatt acttatgttg ccgatcgtcc gggacacgat cgacgttatg
     1021 cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgcgc tggcaccttt
     1261 gggtaatttg attgctcttg atgttcactc tactgattat tgtggtgatt ttagtaaccc
     1321 agaaggtgtg gctgaaaccg tcaaaaaaat tcgtcctgac gttattgtta atgctgctgc
     1381 tcataccgcg gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 aagtgttgaa gcgattgcaa aagcggctaa cgaagttggg gcttgggtaa ttcattactc
     1501 aactgactac gtattccctg gaaattgcga cacgccatgg ctggagacgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agccggagaa aaagcgttac aggaacattg
     1621 cgcgaaacat cttattttcc ggaccagctg ggtatatgca ggtaaaggaa acaattttgc
     1681 caagacaatg ttacgtctgg caaaagagcg cgaagaatta gcggttatta atgatcagtt
     1741 tggtgcgcca acgggcgcag agttgctggc tgattgtacg gcacatgcca ttcgtgtggc
     1801 actgaataaa ccagaagtcg caggcttgta ccatttggta gccagtggta ccacaacctg
     1861 gtacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgctct
     1921 caacaagctc aacgcagtac caacaactgc ctatcctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 gcttggcgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aactaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga ctatggccgt
     2221 cagtaaacag ctgttaccga tttatgataa accgatgatc tattacccgc tttctacact
     2281 gatgttagcg ggtattcgcg atattctgat tatcagtacg ccgcaggata ctcctcgttt
     2341 tcaacaactt ctgggtgacg ggagccagtg ggggctaaat cttcagtata aagtgcaacc
     2401 gagtccagat ggtcttgcgc aggcatttat catcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctctg gttctcggtg ataatatctt ttacggtcat gatctgccga agttaatgga
     2521 tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg aagaaaaacc
     2641 gttacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata acggcgttgt
     2701 cgaaatggcg aaaaatctta agccttctgc ccgtggtgaa ctggaaatta ccgatattaa
     2761 ccgtatctat atggaacagg ggcgtttatc tgttgccatg atgggccgtg gctatgcatg
     2821 gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg caacaattga
     2881 agaacgtcag gggctgaaag tttcctgccc ggaagaaatt gcttaccgta aaggatttat
     2941 cgatgctgag caggtgaaag tattagctga atcattgaag aaaaatgctt atggtaagta
     3001 tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaaca gaaattccag
     3061 atgtactgat ttttgaaccg aaagtttttg gtgatgagcg tggtttcttt atggaaagct
     3121 ttaatcagaa aattttcgaa gaggctgtag ggcggaaggt tgaatttgtt caggataacc
     3181 attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa ccatatgctc
     3241 aaggtaaatt agttcgttgt gttgtcggtg aagtatttga tgtagcagtt gatattcgta
     3301 aatcgtcacc tacatttggg aaatgggttg gggtgaattt gtctgctgag aataagcgtc
     3361 agttgtggat acctgaagga tttgcgcatg gatttttggt gctgagtgaa acggctgagt
     3421 ttgtttataa aacaacaaac tattacaatc caagttttga aaaaagtatt tcatactcag
     3481 atcctaccat taaaattcag tggcccaatt tacaggatat gcattttaaa ttatcaaata
     3541 aggatttgaa tgctaagaat ttttttaata acaatagttt aatgcaatga agaaaaatat
     3601 attgctcttg ttcttagtac atggggcaaa ttatttgttc ccgtttatag ttcttccata
     3661 tcaaactcga atactaagca tcgagacatt cgcagatgta gcaaaaattc aagccgctgt
     3721 gatgctttta tctttaatcg taaattatgg atataactta tcaagtacaa gagctatagc
     3781 tagggctgta tctcaagcag aaataaataa gatctatagt gagactctta ttgtaaaatt
     3841 attattggca accatttgtc ttgcacttgg ttacgtatat ttgatgtatg tcaaagagta
     3901 ctcattgata tatcctttta taatcagttc gatatatctt tatggtagtg cattatttgc
     3961 tacttggtta ttccaaggac ttgagaaaat gaaagcgatc gttatagcaa caacaatcgc
     4021 taaactgaca ggtgtgatac ttacttttat tttagttaag tctccaaatg atatagatgc
     4081 agctcttttt acacaaaaca ttgggatgtt tataagtggt ataatatcta tttatttggt
     4141 aaggaaaaac aaatatgcaa ccgtaatata ttttcgactt aaaaatatta ttttaagctt
     4201 aaaagaagcg tggccgtttt ttttatcatt agctgcaaca agtgcatata catattttaa
     4261 tgtgatttta ttatcttttt atgctggtga ctatgttgtg gcaaatttta atgctgctga
     4321 taaattaaga atggctgctc aagggttgct tattccaata ggacaggctg ttttcccacg
     4381 attatctaaa ctagagggct atgaatatag ttctaaactt aaaatttatg caattaggta
     4441 cgctattttt ggtgtttgca ttagtgcggg acttgtattt ttaggtccca tgttaactac
     4501 tatttattta ggcaaagaat attctttgtc aggagaatat cttcaaagta tgtttttact
     4561 acctgccatt atttcaatat cgactatact gagtcaatgg atgttgatac ctcaaggcaa
     4621 agaaaaaata ttaagcagaa tctatattct aggcgccatt ttccatttat tatatgcctt
     4681 tcctttagtt tactattatg gggcttgggg catggtaata tcaattttat ttactgaagt
     4741 cttaattgta ttatttatgc ttaaggctgt gaaatgactt actttactgg ttttatttta
     4801 atattgtttg ctattataat taaaagatta actccaagtc aaagcaagaa aaatattgtc
     4861 ttaatagcca atgcgttttg gggaatatta ttggtaggtt atgctttcaa tgagcaatat
     4921 ttcgtaccat taagtgcaac aaccttgttt tttatacttg cattcttatt tttctttagt
     4981 atgacttata ttttaattgc taggagtgga agggttgttt tttctttcgg tactggtttt
     5041 atagaaagca aatatattta ctggtttgct gggatgatta atattattag tatctgcttt
     5101 ggcattatcc ttttatataa taatcatttt tctttaaaag taatgagaga aggaatttta
     5161 gatggttctg ttagtgggtt tggtttgggg ataagtttgc cactttcctt ctgctgtatg
     5221 tatttagcaa gacatgagaa taaaaaaaat tatttctatt gttttacact attttcattc
     5281 ttgcttgcgg tgttatcaac ttcaaagatc ttcttaatat tattccttgt atatattgtt
     5341 ggaataaata gttatgtaag caaaaaaaaa ttgcttattt atggaacgtt tgtatttgga
     5401 ctgttcgctt tatcaagtat tatcttgggt aagttctctt cagaccctga aggcaagatt
     5461 atttcagcaa tatttgatac gttaagggtt tatcttttct cgggattggc agcctttaat
     5521 ctttatgttg aaaagaatgt cacgctcccc gaaaatttac ttttgtatcc atttaaggag
     5581 gtttggggga cgacaaaaga tattcccaaa actgatattt tgccttggat caacactggt
     5641 gtatgggaca cgaatgtata tacagctttt gcaccatggt atcagtcatt gggattatat
     5701 gcagctataa ttattggtat tctcttaggg ttttattacg ggatatggtt tagctttcgt
     5761 caaaatttag ctgtgggttt ttatcaaaca ttcttgtgtt ttcctctttt aatgttgttt
     5821 ttccaggagc attatttgtt gtcatggaaa atgcatttta tttatttttt atgtgcaatt
     5881 ttattagcga tgagaaaagc attagagtat gaataaatat tgtatcttag tactatttaa
     5941 tccagatata agtgttttca ttgataatgt caaaaagatt ttatctttgg atgtgagttt
     6001 atttgtatat gacaattcag caaataaaca tgcattcctt gctctatcct cacaagagca
     6061 aacaaagata aattactttt cgatatgtga aaatatcgga ttgtcgaaag cttataatga
     6121 aacactaagg catattcttg aatttaataa gaatgtgaaa aataaaagca ttaacgatag
     6181 tgtgcttttt ctcgaccaag actctgaagt tgatttaaat tccatcaata ttttgtttga
     6241 aactatatca gcagcagagt ctaatgtaat gatagttgca gggaatccca taaggagaga
     6301 tggactaccg tatatagatt acccccacac tgtaaacaat gtaaaatttg taattagtag
     6361 ttatgctgtg tatcgcttag acgcatttag taacatcggc ttgtttcaag aagatttttt
     6421 tatagatcat atcgatagtg atttttgttc aaggctgata aaaagtaatt accaaattct
     6481 ccttagaaaa gatgcctttt tttatcaacc aataggaata aaaccattca ttctctgtgg
     6541 tagatattta ttccctatcc catcacaaca ccgaacatat tttcaaatta gaaatgcgtt
     6601 tttaagttac aggcgcaatg gtgttacatt taatttttta tttagggaaa ttttaaatag
     6661 attgattatg agtatattct caggccttaa cgagaaagac ttatttaaac gattgcattt
     6721 atatttaaaa ggaataaaag atggtcttaa aatgtaattc ttggctagaa gtggggacgt
     6781 tgtgattcaa aaaaaagtgg cggcgataat tataacatat aatccagatc taacaattct
     6841 gcgagaaagt tatacgagtc tatataagca agtcgataaa ataattctta ttgataacaa
     6901 ctctacaaac tatcaagaac ttaagaagtt attcgaaaaa aaagaaaaaa taaaaatagt
     6961 gcccttgagt gataatatag gtctagcagc agctcaaaat ttaggtttga acttagctat
     7021 taaaaataac tatacttatg ctattttatt cgatcaggat agcgtcttac aagacaatgg
     7081 aattaacagt ttcttttttg aatttgagaa attagttagt gaagagaaat taaatatagt
     7141 tgccattggg ccaagttttt ttgacgaaaa gacaggaaga cgctttcggc ctacaaaatt
     7201 tatcggtccc tttttatatc cctttcgtaa aataaccaca aaaaatcctc taacagaagt
     7261 tgacttcttg attgcttctg gttgtttcat aaaattggag tgtattaaat cagccggaat
     7321 gatgactgaa tcgttattca tcgattatat tgatgttgaa tggtcatatc gtatgcgttc
     7381 gtatggctat aagctatata ttcataatga tattcacatg agtcatttag tgggagaatc
     7441 tcgagttaat ttaggattga aaactatttc tttacatggg ccgctaagac gatattactt
     7501 atttaggaat tatatttcaa ttttaaaagt gagttatata ccgttaggat ataaaatacg
     7561 tgagggtttt tttaatatcg gaagattttt ggtgagtatg attataacta aaaatagaaa
     7621 aactttaatt ttatacacta taaaagcaat taaggacgga ataaataatg aaatggggaa
     7681 atataaaggc taacaacata ttatgaaaaa aataatacat atccaagtgt tgccgaaaat
     7741 gtctggggtt cagcaaatct cttttgatat tttgtcaggt cttaaagaca aggatgtaca
     7801 aaaatttata ttatgcggtt taccagatgg ctgttcagat aatgaattta aaaagaaatt
     7861 tactgatata ggtgttaggg ttattacgat acctacctta aaacgaaata tcgggtggca
     7921 tgactttcga tgtttcattg atttatacaa tttttttaaa aaggaaaaat ttgatatagt
     7981 tcatacaaac tcaactaagc caggaataat agctagaata gccgctagat tagccgggac
     8041 aaaactcatt attcacacag tacatgggat cgcatttcat agaaaagaaa atactgtaag
     8101 gaaaattttt tactattgtc tggaaaattt tgcgactctt tttggtagta ttaatgtgac
     8161 agtgaacgag aattatttaa aatattatcc attcgttaaa agtcatatta tatataatgg
     8221 agttgatttc aacgttcttt gctgcaataa aaaggatcat gattttttac atattgcatt
     8281 tatggctaga cttgataaac aaaaaaaccc tttggagttt ataagagccg ttaatattat
     8341 taagaaaaaa ttaccaaatg agcgtttgaa atttacattg gctggctgtg gtgagttaga
     8401 aaatgaatgt aaaaaattaa tagaacattt tcatcttaca gatgttattg atatgcctgg
     8461 atggatagta gataaaaaca cattttataa ctctgtcgat attatttgcc agccatccaa
     8521 ttgggaggct tttggcttag tatttgttga ggccgcattt tttgaaattc catctgtttc
     8581 aaggaatatt gaagggattc ctgaggttat tttagataat gaaactgggc ttttgtatga
     8641 gggtggagaa gccgagttga gtgaaaagct gatatcatta attcatgata aaaagaaaat
     8701 ttcttggtta ggtttgaatg ctaaagaata tgtcttaaag catttcacca aagatatcat
     8761 ggttgaaaaa tattcaaaac tgtatgattt ataagtatct aattaaattt cactatgatt
     8821 tttgcattat gatattatag acccgtgtat tttttcaagc cgcacgccct cgcggtgacc
     8881 accccctgac aggagtaaac aatgtcaaag caacagatcg gcgtagtcgg tatggcagtg
     8941 atggggcgca accttgcgct caacatcgaa agtcgtggtt ataccgtctc tattttcaac
     9001 cgttcccgtg aaaagacgga agaagtgatt gccgaaaatc caggcaagaa actggttcct
     9061 tactatacgg tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg
     9121 gtgaaagcag gtgcaggcac ggatgctgct attgattccc tcaagccata cctcgataaa
     9181 ggtgacatca ttattgatgg tggtaatacc ttcttccagg acaccattcg tcgtaaccgt
     9241 gagctttctg ccgaaggttt taacttcatc ggtaccggtg tttccggcgg tgaagagggg
     9301 gcgctgaaag ggccttccat catgcctggt gggcagaaag aagcctatga actggttgcg
     9361 ccgatcctga ccaaaatcgc cgccgttgct gaagatggcg aaccgtgcgt tacctatatt
     9421 ggtgccgatg gcgcaggtca ctatgtgaag atggttcaca acggtattga atatggcgat
     9481 atgcagctga ttgctgaagc ctattctctg cttaaaggtg gcctgaacct caccaacgaa
     9541 gaactggcgc agacctttac cgagtggaat aacggtgaac tgagtagcta cctgatcgac
     9601 atcaccaaag acatcttcac taaaaaagat gaagacggta actacctggt tgatgtgatc
     9661 ctggatgaag cggctaacaa aggtaccggt aaatggacca gccagagcgc gctggatctc
     9721 ggagaaccgc tgtcgctgat taccgagtct gtgtttgcac gttatatctc ttctctgaaa
     9781 gatcagcgcg ttgccgcatc taaagttctc tctggtccgc aagctctgtc agcaggcgac
     9841 aagcctgagt tcatcgaaaa agttcgccgt gcgctgtatc tgggcaaaat cgtttcttac
     9901 gctcagggct tctctcagct gcgtgctgcg tctgaagaat acaactggga tctgaactac
     9961 ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt cctgcagaaa
    10021 atcaccgatg catatgccga aaatccgcag atcgctaacc tgctgctggc tccgtacttc
    10081 aagcaaattg ccgatgacta ccagcaggcg ctgcgtgatg tcgttgctta tgcagtacag
    10141 aacggtatcc cggttccgac cttcgctgct gcggttgcct attacgatag ctaccgtgcc
    10201 gctgttctgc ctgcgaacct aatccaggca cagcgcgact a
//
