LOCUS       AY647261               11893 bp    DNA     linear   BCT 07-FEB-2005
DEFINITION  Escherichia coli O15 O-antigen gene cluster, partial sequence.
ACCESSION   AY647261
VERSION     AY647261.1  GI:56123303
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Beutin,L., Tao,J., Feng,L., Krause,G., Zimmermann,S., Gleier,K.,
            Xia,Q. and Wang,L.
  TITLE     Sequence analysis of the Escherichia coli O15 antigen gene cluster
            and development of a PCR assay for rapid detection of intestinal
            and extraintestinal pathogenic E. coli O15 strains
  JOURNAL   J. Clin. Microbiol. 43 (2), 703-710 (2005)
   PUBMED   15695667
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   Beutin,L., Tao,J., Feng,L., Krause,G., Zimmermann,S., Gleier,K.,
            Xia,Q. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2004) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 HongDa Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serovar="O15"
                     /db_xref="taxon:562"
     CDS             1215..2351
                     /note="transposase"
                     /codon_start=1
                     /transl_table=11
                     /gene="IS"
                     /product="H-repeat protein"
                     /protein_id="AAV74540.1"
                     /db_xref="GI:56123304"
                     /translation="MELKKLMEHISVIPDYRQAWKVEHNLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELIN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGCDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAASFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            2430..3674
                     /gene="wzx"
     CDS             2430..3674
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAV74541.1"
                     /db_xref="GI:56123305"
                     /translation="MSKTKLNVLYLAISQGANYLLPLLIFPYLVRVIGVSNFGDLSFS
                     LITIQVLLMVVEYGFGYSGTREIALNNDKKYHSEFFCGVVLARFILMLIAAIILIILC
                     FFYVFNDVKYLLCVGFLSVIAGVFNPNWFLQGKEMMSVMAVLSLFSRGIAVVAVYLII
                     KPATPMYISALLLSMPYILYSFCGVAYLLIIKEIFLCRPPIKKIQVILKNGFHFFCST
                     LATSAYTMLTPLVLGGVSGKFDVGIFNSANMIKQGLAGLASPLVQAFYPRINILQREN
                     PYIANLKSRMILKYLLVFYMALAMPFLLFANQLSLLIFGMKGEVIAGAMQLMTLLPIF
                     IGFNTVVGLLVLVPNGMQKQYFKSIFLGTITCLSIVYPACKYYGATGAIVSLIVAEIF
                     VGMGMLKQFIKVNKTVCRPHKL"
     gene            3671..4657
                     /gene="wbuS"
     CDS             3671..4657
                     /gene="wbuS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuS"
                     /protein_id="AAV74542.1"
                     /db_xref="GI:56123306"
                     /translation="MNISVIISVWKRPVQLELILSELDSQAKDNSLHLEVIVSDSHSG
                     KEIDDVVADNIHKKKNINIIHQHTKNILSAKRNFGASLAHGDYLIFLDDDCIPASGYI
                     SSLLNYLKKMNSKSVLCGEVRFENELIETSNYYRYRNSLHPKFSDSHDISMNAWTFVA
                     MNCVLDRKAFSSGIVSYNENFIGYGCEDHEFGWQLEKNDFKIIFADFKILHHEYSGDI
                     EGYTKKIRATARDGMNVLSKVRPEMFSTNKKLFLVEKIFSKHKMFSKICQSIFFNKFI
                     FKKIIQFLKKTDANKKLYFPILYRYVLISAYIHGIGERGTSKTDELLKNWYI"
     gene            4707..5885
                     /gene="wzy"
     CDS             4707..5885
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAV74543.1"
                     /db_xref="GI:56123307"
                     /translation="MKYNALMAFLLFFVVFFRLSLIIPFLYLAFIPAFFGIMYLVRNF
                     MITMGNGLVSIDRKNLLLLSIFIIIFLFCMVFDSFQKSQSFQSYFTVRLFMLFLFSFV
                     PAYYLVNRFIKGDLKLMERILVYSLWVQIVIFFGMYISPELKRLLYTFFGMSDSVNLW
                     EQNAKVRGFGLSGEINFMTPFLMIYMSFFMMKRRYALITLICLTQIVNSNMAVIAAII
                     GIGCSRLNINIKIATVLVLGVLVYSLGAVFFPRFYDEFVSGDGTRTLDILLQQHVFVV
                     GNLDFFNIIFGLQQNISSSIPDIKQSSDMGWVILFNYGGLTFITLFLFLIFTISIATF
                     GMTYQAIIWMLIGIIFNTKGLVLGSNGYFFLSFIYMFLNRVTLSGQSSITNKLGQVSK
                     "
     gene            5927..6961
                     /gene="fnlA"
     CDS             5927..6961
                     /gene="fnlA"
                     /note="L-fucosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="AAV74544.1"
                     /db_xref="GI:56123308"
                     /translation="MFSSKTLLITGGTGSFGNAVLNRFLDTDIAEIRIFSRDEKKQDD
                     MRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNIL
                     GTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDRNKTVIC
                     GTRYGNVMASRGSVIPLFVDLIRAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIDTLAIALKELLNVPDHPVNVIGTRHGEKLYEALLSREEMIAAIDM
                     GDYYRVPPDLRDLNYGKYVEQGDSRISEIEDYNSHNTQRLDVEGMKELLLKLAFIRAI
                     RAGEKYNLDS"
     gene            6963..8066
                     /gene="fnlB"
     CDS             6963..8066
                     /gene="fnlB"
                     /note="L-fucosamine"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="AAV74545.1"
                     /db_xref="GI:56123309"
                     /translation="MKILVTGANGFIGRNLCLRLEELGYKDLIRIDRESTKQDLEQGL
                     QDADFIYHLAGINRPKTDDEFISGNSDLTKHIVEYLLSIGKNTPIMLSSSIQAELNNA
                     YGVSKAVAESYVQKYAAASGSSYYIFRYPNVFGKWCKPNYNSFIATFCYNISNDIEIT
                     INDASAPVKLVYIDDVCTDAIALLSGTVESGYKVVAPIYSTTVGEVAELIYSFKNSRS
                     TLITEAVGAGFTRALYSTWLSYLPAEKFAYKVPFYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELQVSSGEFKIVETVPGWT
                     HDITNIGTDELIVMLWANEIFNRDEPDTIARPL"
     gene            8066..9196
                     /gene="fnlC"
     CDS             8066..9196
                     /gene="fnlC"
                     /note="L-fucosamine"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="AAV74546.1"
                     /db_xref="GI:56123310"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHIIVHTGQSYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMFEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            9196..10407
                     /gene="wbuB"
     CDS             9196..10407
                     /gene="wbuB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuB"
                     /protein_id="AAV74547.1"
                     /db_xref="GI:56123311"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRAINETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGHLVKKIKARCQCPAYLILRDMFPQWVIDAGMLNAGSPIERYFRLFEKI
                     SYRQANFIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAAYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNSPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGSGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            10394..10792
                     /gene="wbuC"
     CDS             10394..10792
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="AAV74548.1"
                     /db_xref="GI:56123312"
                     /translation="MRLIDNDQLDELYDQAGQSERLRSHLMMHGSHQEKVQRLLIALV
                     KGSYVEPHYHELPHQREMFIVMEGQLKVCLYGRNGEVIKQFIAGDNTGISIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDPSFAKSFV"
BASE COUNT     3536 a   2049 c   2578 g   3730 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttatgtg cacgacccgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcacga ttcaacgaaa cgggtcgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agaaccactg
      421 gaccgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgttggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcacgcagcc aggtgcatgg gggcgtattc agctgactga tgccatcgct
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcattt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccataaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgagc taagcgcgag tgggtaacgc tcgtcacatc gtagacatgc
     1021 atgcagtgca ctggtagctg taaagccagg agcggtagcg tgtctagggg cagggaaaga
     1081 ttatcagaag tacacttttt gtactaaata attcgcattt tatgttcaaa aattgagata
     1141 tccctcatta cctaaaactg tttttcacag cttatatatg atcaaatact ccttgcttaa
     1201 ataaggagaa caaaatggaa cttaaaaaat tgatggaaca tatttctgtt attcccgatt
     1261 acagacaagc ctggaaagtg gaacataatt tgtcggatat tctactgttg actatttgtg
     1321 ccgtcatttc tggtgcagaa ggctgggaag atatagagga ttttggggaa acacatctcg
     1381 attttttgaa gcaatatggt gattttgaaa atggtattcc tgttcacgat accattgcca
     1441 gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac tggatgcgtg
     1501 actgccattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg ctccggcact
     1561 cttatgacaa gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc
     1621 acagtctggt catcggacag atcaggacgg atgagaaatc caatgagatt acagctatcc
     1681 ctgaacttat taacatgctg gatattaaag gaaaaatcat cacaactgat gcgatgggtt
     1741 gccagaaaga tattgcagag aagatacaaa aacagggatg tgattattta ttcgcggtaa
     1801 aaggaaacca ggggcggcta aataaagcct ttgaggaaaa atttccgctg aaagaattaa
     1861 ataatccaga gcatgacagt tacgcaatta gtgaaaagag tcacggcaga gaagaaatcc
     1921 gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag
     1981 gactgaaaaa attatgtgtg gcagcctcct ttcggtcaat aatagcagaa caaaagaaag
     2041 agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgca gagaagttcg
     2101 ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg
     2161 taatgaatga agacgactgc aaaataagaa gaggaaatgc agcagaatta ttttcaggga
     2221 tacggcacat cgctattaat attttgacga atgataaggt attcaaggca gggttaagac
     2281 gtaagatgcg aaaagcagcc atggacagaa actatctggc gtcagtcctt gcggggagcg
     2341 ggctttcgta atcttgccct gaataaacaa cttattgatt catgtgaata agttactcga
     2401 atgatatctg ttgtatatgg ggcacttcat tgagcaaaac taaactaaat gttctttacc
     2461 ttgcaataag tcagggtgcc aattacctac tgccattatt aatttttcct tatcttgtta
     2521 gagtcattgg tgtatcgaat tttggtgatc tgagtttttc attgataact atacaagtgt
     2581 tgttaatggt tgttgaatat ggttttggat atagtgggac aagagaaata gcactaaata
     2641 acgataaaaa ataccattct gaattttttt gcggtgtggt gcttgctcgt tttatattaa
     2701 tgctaattgc agctattata ctcataatac tctgtttttt ttatgttttt aacgacgtta
     2761 agtatttgtt atgtgttggt tttctgtccg taattgcagg tgttttcaat ccaaattggt
     2821 ttttgcaagg taaggaaatg atgagtgtga tggctgtgct gtcactattt tcacgaggca
     2881 tagcagtcgt tgcagtttat ctaattataa aacccgcaac gccgatgtac atcagtgcct
     2941 tattattgag catgccatat attttgtatt cattctgtgg cgttgcctac ttacttatta
     3001 tcaaggagat ttttttatgt aggccaccga taaagaaaat tcaagtaatt ttaaaaaatg
     3061 gatttcattt tttttgttca acacttgcga ctagtgcata cacaatgttg acccctcttg
     3121 tattgggtgg cgtatctgga aagtttgatg taggcatctt taactcagct aacatgatca
     3181 aacaaggttt ggctggactt gcatcaccat tagtccaagc tttttatcca agaattaaca
     3241 ttttgcaaag agagaatcca tatattgcaa acttaaaatc tagaatgatt cttaaatact
     3301 tgcttgtttt ttacatggct ttagcaatgc catttttact ttttgccaac caattatcat
     3361 tattaatatt cggcatgaaa ggtgaagtaa ttgcaggtgc aatgcaatta atgacattgc
     3421 ttcctatatt cataggtttt aatacagttg tcgggttact tgtattagta cctaatggga
     3481 tgcaaaaaca gtatttcaaa tctattttcc taggaactat tacttgttta agcatagttt
     3541 atccagcatg taaatattat ggagcaacgg gtgcgattgt gagtcttatt gtagctgaaa
     3601 ttttcgttgg catgggaatg cttaaacaat tcattaaagt aaataaaact gtatgtaggc
     3661 ctcataaatt atgaatatct cggtaataat atctgtttgg aaacgcccag ttcaattaga
     3721 attgattctc tctgagctcg attctcaggc taaagacaat agtctacacc tagaagtaat
     3781 tgtttccgat agtcatagtg gtaaagaaat tgatgatgta gttgctgata atattcataa
     3841 aaagaaaaat attaatatta tccatcaaca tactaaaaat atactctccg ctaagcgcaa
     3901 tttcggagca tccctagccc atggggatta tttaatattt cttgatgatg attgtatacc
     3961 cgcaagtgga tatatatcat cgttgctgaa ctatttaaaa aaaatgaata gtaaaagcgt
     4021 attatgtggg gaagttagat tcgaaaatga actcattgag accagcaatt actatcgcta
     4081 caggaactct ttacacccta agtttagtga tagtcatgat atctctatga atgcctggac
     4141 ttttgtcgca atgaattgtg ttcttgatag aaaggcattt tcatcaggta tagtttcata
     4201 taatgaaaat tttattggtt atggttgtga agatcatgag tttgggtggc aacttgaaaa
     4261 aaatgacttc aaaattattt ttgctgattt taaaatatta catcacgaat acagtggcga
     4321 tatagaagga tatacaaaaa aaattcgtgc tacagcacgt gatggtatga atgtattaag
     4381 caaagtaaga cctgaaatgt tttctactaa taaaaaatta ttcctggttg agaaaatatt
     4441 tagtaaacac aaaatgttta gtaaaatatg ccaatcaata tttttcaata aatttatttt
     4501 taaaaaaata atacaatttt taaaaaaaac agatgcaaat aaaaaactct atttcccaat
     4561 tctttacaga tatgtgttga tttcggcata tatacatggt attggagagc gtggcacctc
     4621 aaaaacagat gaattgctta agaactggta tatatagatg atgctatctt catttattaa
     4681 gacatttgta tggaaggtaa aagacaatga agtataatgc attgatggct tttttattat
     4741 tttttgttgt tttttttaga ttgtcgctga taataccttt cttatatttg gcatttattc
     4801 ctgcattttt tggtattatg tatttagtgc gtaattttat gattactatg ggcaatggat
     4861 tggtatctat agatcgtaaa aatttgttgc tgttatctat attcataatt atttttttat
     4921 tttgtatggt tttcgattcg tttcaaaaaa gccaatcttt tcaaagctat tttaccgtta
     4981 gattatttat gttgttttta ttttcatttg ttcctgcgta ttatttagta aatagattca
     5041 taaagggtga cttgaaatta atggagcgaa tattagtgta ttctctctgg gttcaaatag
     5101 ttattttttt tggtatgtat ataagtccag agttaaaaag attgttatat actttctttg
     5161 gtatgtctga ctcagttaat ctttgggaac aaaatgctaa agtaagagga tttgggttgt
     5221 cgggtgaaat aaatttcatg acaccatttt tgatgatcta tatgtcattt tttatgatga
     5281 aaaggcgtta tgctttaatt actttaattt gtcttactca aatcgtaaat tctaacatgg
     5341 ctgtgattgc agccattatt ggtatcggtt gctctagact taatattaat ataaaaattg
     5401 caacagtatt ggttttggga gttttagttt atagcttagg agcggtgttc ttccctcgat
     5461 tttatgatga gttcgtttct ggagatggca caagaactct ggatatctta ttacagcaac
     5521 atgtgtttgt tgtaggtaat ttagattttt ttaatattat atttggatta cagcaaaaca
     5581 tatcttcatc aatccccgat attaaacaaa gttcggatat gggctgggtt atactgttta
     5641 attacggtgg gttaacattt attacactct ttttattttt aatctttact atttctattg
     5701 cgacatttgg aatgacatat caagcaatta tatggatgtt aattgggata attttcaata
     5761 ccaaaggttt agttttagga tctaacggct atttctttct atcttttata tatatgtttt
     5821 tgaatagagt aacacttagt ggacagagtt caattactaa taagttaggc caagtaagta
     5881 aatagcttcc agagtatatt tgtcaataat ttgaggttcg gttattatgt tttcatctaa
     5941 aacactgtta attactggtg gtactggctc tttcgggaat gctgtattaa atagatttct
     6001 tgatacagat attgcagaaa tccgtatatt tagtcgtgat gaaaaaaaac aagatgatat
     6061 gcggaaaaaa tacaataatc aaaaattaaa gttctatatt ggtgatgtca gagattaccg
     6121 tagtattttg aatgcgactc gcggtgttga ttttatatat catgcagcgg cacttaagca
     6181 agttccatca tgtgaatttc atcctatgga agccgttaaa actaatatcc ttggtacgga
     6241 aaatgttctt gaagcagcta tagcgaatga agtgaagagg gttgtatgcc taagtactga
     6301 taaagctgta tacccgatta acgcaatggg tatttcaaaa gctatgatgg aaaaggtcat
     6361 ggtcgcgaaa tcccgtaatg ttgatcgcaa taaaacagta atatgtggta cccgttatgg
     6421 gaatgttatg gcatctcgcg gttcagttat tccattattt gttgatctta ttagagcggg
     6481 caagccactc acaataactg atcctaatat gacccgcttt atgatgactc ttgaggatgc
     6541 ggtagattta gttctttatg cgtttgaaca tggtaataat ggtgatatct ttgtgcaaaa
     6601 agcacctgca gcaactattg acacattagc tattgcttta aaggaattac taaatgttcc
     6661 agaccatccg gtaaatgtca ttggaacgcg tcatggcgag aaattatatg aagctctact
     6721 tagtcgtgag gaaatgatcg ctgctataga tatgggtgat tattaccgtg tcccgccaga
     6781 tcttcgtgac cttaattatg gcaaatatgt tgagcaaggt gatagccgaa tatctgaaat
     6841 agaagattat aactctcata atactcaacg gttagatgtt gaaggcatga aagagctctt
     6901 gctaaaatta gcctttattc gagcaattcg tgctggtgaa aaatataatc tggattcatg
     6961 atatgaaaat attagttact ggtgcaaatg gttttattgg tcgtaattta tgtttgaggc
     7021 ttgaggaact tggttataaa gatcttatta gaatcgatcg agaatcaacg aagcaagatc
     7081 ttgaacaagg cttacaggat gccgatttta tttatcactt agctggtatc aatagaccta
     7141 agactgatga tgagtttatt tctggaaaca gtgatttaac aaagcatata gttgagtatc
     7201 tcctttctat tggtaagaat acaccaatta tgctaagttc ttcgatacaa gctgaactta
     7261 ataatgctta tggggttagc aaagctgtag ctgaaagcta tgtccaaaaa tatgctgctg
     7321 ctagtggttc ttcgtattat attttcagat atccaaacgt ttttggtaaa tggtgtaagc
     7381 caaactataa ttcttttata gcaacttttt gctacaatat ttccaatgat attgagatta
     7441 ctatcaatga tgcatcagcg ccagtcaaac tggtttatat tgatgatgtt tgtactgatg
     7501 ctatagctct tctctctggg acggttgaaa gcggatataa agttgttgca ccaatttatt
     7561 caacaacagt tggtgaagtt gcagaattaa tttatagctt caaaaatagc cgttccaccc
     7621 tgatcacaga ggctgtcggg gcgggattta cccgtgcatt gtattctaca tggctgagtt
     7681 atttaccagc agagaagttt gcgtacaagg taccttttta tggggatgcc cgcggagtct
     7741 tttgtgagat gttgaaaacg ccttcagcgg ggcagttttc attttttact gctcaccctg
     7801 gtattacgcg tggcggacat taccatcaca gtaaaaatga gaagtttttg gtcattcgag
     7861 gtcaggcatg ctttaaattt gaacatgtga ttaccggtga gcgatatgaa ctgcaagttt
     7921 catcgggtga gtttaagatt gttgaaacag ttcctggttg gacacatgac attacaaata
     7981 ttggaactga tgaattaata gtcatgctct gggcaaatga aattttcaac cgtgatgagc
     8041 ccgatactat tgcgagacct ctataatgaa aaaattaaaa gttatgtctg ttgttggaac
     8101 ccgtcctgag attatccgtt tgtcgagggt tcttgctaag tttgatgaat actgcgagca
     8161 tattattgtc catactggtc aaagttatga ttacgaatta aatgaagtgt tcttcaatga
     8221 cttgggtgtt cgaaaacctg attatttttt aaatgcagcg ggtaaaaatg cggcggaaac
     8281 cattggccag gttattatta aggtagatga agtattagaa atcgaaaaac ctgaagcaat
     8341 actggtattg ggcgatacga attcatgtat ttctgccatt ccggccaaac gccgtaaagt
     8401 gcctatattt catatggaag caggtaaccg gtgtttcgat caacgcgtgc ctgaagaaac
     8461 caacagacgt attgttgacc atacggctga tatcaatatg acctacagtg atattgctcg
     8521 tgaatatctc ttggctgaag gtatcccagc tgatcggatc ataaaaactg gtagccctat
     8581 gtttgaggtt ctttcatatt atatgcccca aattgatggt tcagatgtgc tatcgcgttt
     8641 gaatctacag tctggtgagt tttttgtagt aagtgcgcat cgtgaagaga atgttgattc
     8701 gccaaaacag ctcgtaaagc ttgcgaacat tctaaatact gttgctgaaa aatataacct
     8761 tccagttatt gtctccacac acccaaggac acgtaaccga atccgtgagc aaggaattga
     8821 atttcattca aatataaatc tactaaaacc attgggtttc catgattata accacttgca
     8881 gaagaactca cgagctgtgc tttcagatag cggtactatc actgaagagt catccatcat
     8941 gaatttccca gcggtaaaca tccgggaagc gcatgagcgt ccggaaggct ttgaggaagc
     9001 atccgtcatg atggtggggt tagagtgtga acgcgtatta caagcgctgg atattctggc
     9061 aacacaaccg agaggtgaag tccgtctttt acgtcaggtt agtgattaca gcatgccaaa
     9121 tgtgtcggat aaagttgtca gaattgttca ctcttacaca gattatgtta agagagtcgt
     9181 ctggaaagaa tattgatgaa acttgcttta atcatagatg attacctgcc caacagtact
     9241 cgtgttggtg caaaaatgtt tcatgaactt gctcaagaat ttatccagcg tgggcacgat
     9301 gttacggtaa ttactcctgg tacgggcatg caagaagaga tttcttttga tacctttcag
     9361 ggggtaaaaa catggcgttt taaaagcggg ccgctcaagg atgtaagtaa aattcagcga
     9421 gcgatcaatg aaacgctttt gtcctatcgg gcgtggaaag ccatcaaaaa atgggtaaaa
     9481 aaagagacct ttgagggggt gatttattat tcaccttcca tattctgggg gcatttagtt
     9541 aaaaaaatta aagctcgttg ccaatgtcct gcttatctta ttttaagaga tatgtttcca
     9601 caatgggtaa ttgatgcagg aatgcttaat gctggttccc caatagaacg ctactttcgt
     9661 ctttttgaaa aaatatctta tcgtcaggca aattttattg gacttatgtc tgataagaat
     9721 cttgatgttt ttcggaaaga taataaaggc tatccgtgcg aagttttgcg taattgggcc
     9781 tccctaacac caacgatcat acccaaggat tatataccac tacgtaagcg acttggccta
     9841 gaggataaaa ccattttctt ctatggtgga aacataggtc atgcacagga catgacaaac
     9901 ttgatgcgac ttgtgagaaa catggcagca tatcctcaag ctcatttcct atttattggc
     9961 cagggggatg aagttgaatt aattaattca ttagcctctg agtgggcatt gacgaatttc
    10021 acctatttgc cctcggttaa tcaggatgaa tttaagttca ttttgtcgga aatggatatc
    10081 ggcttgtttt ctctttccgc taggcactct tcccataatt ctcctggtaa gttattaggc
    10141 tatatggttc agtcgctacc tattttaggt agcgtaaatg ccggaaatga tttgctcgac
    10201 attgtcaatc aaaataatgc cggattaatc catgtcaatg gtgaggatga taaattatgt
    10261 caatctgcgc tattaatgtt gcatgatatt gatgtgcgcc ggcaacttgg ttcgggggcg
    10321 aatatattgt tgaaagaaca attctccgtt gagtctgcgg cacagacgat agaaatgagg
    10381 ttggaggcat gcaatgcgat taattgataa tgaccaactc gacgaattat atgatcaagc
    10441 cgggcaatcg gaacgtttac gttcccacct tatgatgcac ggctcgcatc aagaaaaggt
    10501 acagcgttta cttattgcat tagtaaaggg cagctatgtt gaaccgcatt atcacgaact
    10561 tcctcatcag cgggaaatgt tcattgttat ggaggggcaa cttaaggttt gtttatatgg
    10621 tagaaatggt gaggttataa agcaatttat agcaggagat aatactggaa taagcattgt
    10681 ggagttttct ccgggcgata tacacagtgt cgaatgccta tctccgcgtg ctcttatggt
    10741 ggaagttaag gaggggccat ttgacccttc ttttgcaaaa tcgttcgtgt gatgcttgtc
    10801 taaagtacat cttctgctat ctacccaagc taaacctgag ttaacatcca taccatattt
    10861 caagctgcgc atatcttgcg cggtgaccac ccctgacagg agtaaacaat gtcaaagcaa
    10921 cagatcggcg tcgtcggtat ggcagtgatg gggcgcaatc ttgcgcttaa catcgaaagc
    10981 cgtggttata ccgtctctat tttcaaccgt tcccgtgaaa agacggaaga agtgattgcc
    11041 gaaaatccag gcaaaaaact ggttccttac tatacggtga aagagttcgt tgaatctctg
    11101 gaaacgcctc gtcgcatcct gttaatggtt aaagcaggtg caggcacgga tgctgctatt
    11161 gattccctca aaccatatct cgataaaggt gacatcatca ttgatggtgg taacaccttc
    11221 ttccaggaca ctattcgtcg taaccgtgag ctttctgcag aaggctttaa cttcatcggt
    11281 accggtgttt ccggcggtga agagggggcg ctgaaagggc cttccattat gcctggtggg
    11341 cagaaagaag cctatgaact ggttgctccg atcctgacca aaatcgccgc cgttgctgaa
    11401 gatggcgaac cgtgcgttac ctatattggt gccgatggcg cgggtcacta tgtgaagatg
    11461 gttcacaacg gtattgaata cggtgatatg caactgattg ctgaagccta ttctctgctt
    11521 aaaggtggcc tgaatctttc caacgaagaa ctggcgcaga cgtttaccga gtggaataac
    11581 ggtgaactga gcagctacct gatcgacatc accaaagaca tcttcactaa aaaagatgaa
    11641 gacggtaact acctggttga tgtgatcctg gatgaagcgg ctaacaaagg taccggtaaa
    11701 tggaccagcc agagcgcgct tgatctcggc gaaccgctgt cgctgattac cgagtctgtg
    11761 tttgcacgat acatctcttc tctgaaagag cagcgtgttg ccgcatttaa agttctttct
    11821 ggtccgcaag cgcagcctgc tggcgacaaa ggtgaattca tcgaaaaagt tcgccgtgcg
    11881 ctgtatctgg gta
//
