LOCUS       HM204926               15673 bp    DNA     linear   BCT 01-JUN-2011
DEFINITION  Escherichia coli serogroup O151 O-antigen gene cluster, complete
            sequence.
ACCESSION   HM204926
VERSION     HM204926.1  GI:312162700
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Liu,B., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Feng,L., Shashkov,A.S., Wang,L. and Knirel,Y.A.
  TITLE     Structural and genetic relationships between the O-antigens of
            Escherichia coli O118 and O151
  JOURNAL   FEMS Immunol. Med. Microbiol. 60 (3), 199-207 (2010)
   PUBMED   21039922
REFERENCE   2  (bases 1 to 15673)
  AUTHORS   Liu,B., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Feng,L., Shashkov,A.S., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin,
            Tianjin 300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O151"
     gene            1198..2556
                     /gene="rib"
     CDS             1198..2556
                     /gene="rib"
                     /note="bifunctional ribulose 5-phosphate
                     reductase/CDP-ribitol pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="Rib"
                     /protein_id="ADQ37319.1"
                     /db_xref="GI:312162701"
                     /translation="MKNIAVILASGTGSRFGAKIPKQFVKLAGKPVIQYTLHAFQNAK
                     KIDEIIVVTNEEYVDFVSALTMEHNYFKVTKIIHGGKERYESSWAALQSIELDECNVI
                     FHDAVRPFVSQKIIDDCIDGLNNYNAIDVVVDPTDTIVKIKNKEIMSIPDRRFLARGQ
                     TPQGFKKSLIEKAYIDFLKEEKKVASDDCGIFLKYMPNEPIGIIKGDESNFKITHQQD
                     IYLADNLIKDGLLELISENNDSIARTISEKVIVIFGGNSGIGKTLLVKAKSLDGNVYS
                     FSRSNGIDISNAMNVRKALQSVFEKEGRIDYIINTAAVLIKKPINFMSDEDIEQSCNT
                     NYLGAINIAREGYGYLKKTKGMLINFTSSSFTRGRASYSLYSSSKAAVVNLTQALSEE
                     WLNLGIKVNCINPERTDTPMRRVNFGIEPPESLLTSEQVADMTLSIMSSDYTGQVVAV
                     KK"
     misc_feature    1198..14216
                     /note="O-antigen gene cluster"
     gene            2561..3781
                     /gene="wzx"
     CDS             2561..3781
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADQ37320.1"
                     /db_xref="GI:312162702"
                     /translation="MSLRKNISGLAIAQIFSYIAPLLQFPYLSRIVGDSYLGAIIFIL
                     SISQMAMVLTDYGFDLSIGEQIARGKRNNKWLSVFYCQTGVIKSFLLVVAFVFIISVI
                     RLSKSDIPEQLIIGLMICVTLNAYNPYWIFQGLEKIYIYSALVIISRLIGLVLIYLLI
                     RTPDDYVYYSWILAFQAGMTTIISHIIISRWGVRVCRIKVKGIIKQFKYSFDFFLSRA
                     CVSLYSSGCSVFLATFGGSLNQVAIYGVAEQLYKAGVQVFSPVISALTPYMVRTKNYI
                     IFYKIIVVCITVTVIGMCVGFLFGAQIITLIYGNGYQESKSVLDVFMVVILASVFGML
                     LGYPALLPLGLGKVANYSVIASGILQIIMFCILYIGLFPITAISVCLTYLICDWFMFF
                     IRLYNFMKYRKING"
     gene            3774..4592
                     /gene="wdbI"
     CDS             3774..4592
                     /gene="wbdI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdI"
                     /protein_id="ADQ37321.1"
                     /db_xref="GI:312162703"
                     /translation="MDNQLRAAQMRMLAILNEVDRICRLNKINYWLDAGTLLGAKRHG
                     GFIPWDDDIDICMLRDDYNKFIMACDKQLNKEIFFLQTAYSDEYYVDFNTPCKIRMNN
                     TEIIEKSEVKYKYYDERSHHGLFLDIFPYDKYSSNKYIRKYVERFISQLCKLKVLSKY
                     SGFPITKKILIKCLSSIICKRRLLAFVDLVSFRMSERKHDYLLGAGIETPSHRAYFSE
                     DTIFPLSEIKFGGRMYLCPNNVDKYLCMMFGDNYMTIPPAAQRVWHYHSINIGK"
     gene            4599..5699
                     /gene="wzy"
     CDS             4599..5699
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADQ37322.1"
                     /db_xref="GI:312162704"
                     /translation="MVEGFVIYNSIVLLALLFCYLKRFFLPRYFTVCLFLVLWVPLAL
                     RYGIGRDYFSYIEIYKNVLVARDGIELGFYYINYILAYFNFHYQYLFALSSFITVYFC
                     VKSFDRDYTLVSIILFCALIYLQAFSIIRQMIAVSLCLYSCSLWNKGCKCKSSVFLIL
                     APLFHFSAIIILLLAIASRYIKINTTRCYFVFVFALVFIFILNGVDFIFSNAILLDSK
                     YGYYVTSAFNRPTEIGSGIGVAIALFLPFMVFIKSTQLYEYNKNYNFMLLINLVYVIS
                     YTLSLKIYIFARFTDALSFVQILLIPVILKISSPKGIYYNLMMAVILLHILLFEANLN
                     ANTISAGNISNSGLGIMPYKSILNTKIVGIYD"
     gene            5692..6801
                     /gene="wdbJ"
     CDS             5692..6801
                     /gene="wdbJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdbJ"
                     /protein_id="ADQ37323.1"
                     /db_xref="GI:312162705"
                     /translation="MTSKILFITPLPPPVTGQSLACIELYNYLDKDNNCIIDVINLSK
                     QSFISGKGSFKRVAEIAGVLLRLLFLRRDYDLIYFTPAESKMGSYKDQLIYLFMFAKL
                     KKMYIHLHGGAGMKKLLEKDKSLIFKLNKYFISRLAGVIVLGERLKSIYNGIIESDKI
                     YAVENFSQDYLFIRQSELKLKFNNRNKIKILFLSNLLPGKGYIELLKSADFLDDSCVE
                     FHFAGGFEDEQAKKIFLEAINLKNNIFYHGIVSGDEKRNLLIKSDIFCLPTYYPYEGQ
                     PISILEAYAAGCVVLTTNHSGIFDVFEPWRNGIEVDKKSTSDLVNAINYFNSNREEIR
                     KIAINNIVDARKKYRLKHHLEKMMTVLMNHEVIQQ"
     gene            6798..7934
                     /gene="wbuX"
     CDS             6798..7934
                     /gene="wbuX"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuX"
                     /protein_id="ADQ37324.1"
                     /db_xref="GI:312162706"
                     /translation="MKKYKICNRCVMDTSDPNISFDDNGQCDYCSNFDKNILPNWNAN
                     NSGLSKLLEMSESIKKDSKGSDFDCIIGLSGGLDSSYAAYIAKEKMGLRPLLFHVDAG
                     WNTDQAVGNIEKLIEGLGLDLYTEVINWEEMKDLQLSFLKSGIPDQDLVQDAAFFSSL
                     YKFARKHKIKHVITGSNFSTECCREPEEWGGYLGIDTMLFNDIHRQFGTRPLNSFPLV
                     DILIYKVFYQKILGMKVHHPLNLVPFTKKDAENELSEKFGWQPFQHKHHESRFTRFYE
                     DYWLPRRFGYEKRRAHFSSLIMTGQMTREQALERLLKPEMDEHFLEQEFEYIAHKLGI
                     SVDDLKELFSIPKKTYKDYKNKRWLISLGTNILRTLGLEKRYFR"
     gene            7931..8557
                     /gene="wbuY"
     CDS             7931..8557
                     /gene="wbuY"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuY"
                     /protein_id="ADQ37325.1"
                     /db_xref="GI:312162707"
                     /translation="MIKIIDYGIGNIQAFLNVYKRLGINSGIAHSIDDIKSATHLILP
                     GVGAFDQAMTLFNNSGLRESIEKRIFEDRVPIIGICVGMQMLATSSEEGSLPGLGWIP
                     GTVRAFSSNISSRSLPMPHMGWNSLIKKVNTPLLKDFKEESSFYFLHSFYYECDDAGD
                     VLATAEYGHNFHCVIGRNNIYGIQCHPEKSHSSGYQLLKNFAEIQYNA"
     gene            8550..9338
                     /gene="wbuZ"
     CDS             8550..9338
                     /gene="wbuZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuZ"
                     /protein_id="ADQ37326.1"
                     /db_xref="GI:312162708"
                     /translation="MLRPRIIPCLLVHDSGLVKTVNFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSRGLEPNYALIKKLAAECRMPLCYGGGVTSVAQAVKIISLGVEKVSI
                     SSAAVENPNIVKDLAEAVGKQSVVVVLDVIKRKGLFSKGYELSTRNNTRTYKIDPISF
                     AQKMVALGAGEIVINFVDNDGVMGGYDVEYSSTIKSQINVPVTFLGGAGNYEHLSALI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTPEQKDIICNNLKKNY"
     gene            9348..10385
                     /gene="fnlA"
     CDS             9348..10385
                     /gene="fnlA"
                     /note="4,6-dehydratase, 3- and 5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ADQ37327.1"
                     /db_xref="GI:312162709"
                     /translation="MMFKDKILLITGGTGSFGNAVLNRFLSTDIKEIRIFSRDEKKQD
                     DMRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNI
                     LGTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDRNKTVI
                     CGTRYGNVMASRGSVIPLFVDLIRAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGN
                     NGDIFVQKAPAATIDTLAIALKELLNVPDHPVNVIGTRHGEKLYEALLSREEMIAAID
                     MGDYYRVPPDLRDLNYGKYVEQGDSRISEIEDYNSHNTQRLDVEGMKELLLKLAFIRA
                     IRAGEKYNLDS"
     gene            10387..11490
                     /gene="fnlB"
     CDS             10387..11490
                     /gene="fnlB"
                     /note="reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="ADQ37328.1"
                     /db_xref="GI:312162710"
                     /translation="MRILVTGANGFIGRNLCLRLEELGYKDLIRIDRESTKQDLEQGL
                     QDADFIYHLAGINRPKTDDEFISGNSDLTKHIVEYLLSIGKNTSIMLSSSIQAELNNA
                     YGVSKAVAESYVEKYAAASGSSYYIFRYPNVFGKWCKPNYNSFIATFCYNISNDIEIT
                     INDASAPVNLVYIDDVCTDAIALLSGTVESGYKVVAPIYSTTVGEVAELIYSFKNSRS
                     TLITEAVGTGFTRALYSTWLSYLPAEKFAYKVPFYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELKVSSGEFKIVETVPGWT
                     HDITNIGTEELIVMLWANEIFNRDEPDTIARPL"
     gene            11490..12620
                     /gene="fnlC"
     CDS             11490..12620
                     /gene="fnlC"
                     /note="C-2 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="ADQ37329.1"
                     /db_xref="GI:312162711"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHIIVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMYEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            12620..13831
                     /gene="wbuB"
     CDS             12620..13831
                     /gene="wbuB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuB"
                     /protein_id="ADQ37330.1"
                     /db_xref="GI:312162712"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRAVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGHLVKKIKARCQCPAYLILRDMFPQWVIDAGMLNAGSPIERYFRLFEKI
                     SYRQANRIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAVYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGLGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            13818..14216
                     /gene="wbuC"
     CDS             13818..14216
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ADQ37331.1"
                     /db_xref="GI:312162713"
                     /translation="MRLIDNDQLDKLYDQAGQSERLRSHLMMHGSHQEKVQRLLIALV
                     KGSYVEPHYHALPHQWEMFIVMEGQLQVCLYGRNGEVIKQFIAGDNTGISIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDSSFAKLFV"
BASE COUNT     4827 a   2547 c   3299 g   5000 t
ORIGIN
        1 gctgcaggga tcaaagaaat cctcctggta acacatgcgt ccaagaacgc agtcgaaaac
       61 cacttcgaca cctcttatga attagaatct ctccttgagc agcgcgtgaa gcgtcaactg
      121 cttgcggaag tgcagtccat ctgtccgccg ggcgtgacca ttatgaacgt gcgtcagggc
      181 gaacctttag gtttgggcca ctccatttta tgtgcacgac ctgccattgg tgacaaccca
      241 tttgtcgtgg tactgccaga cgttgttatc gatgacgcca gcgccgaccc gctgcgctac
      301 aacctggctg ccatgattgc gcgcttcaac gaaacgggcc gcagccaggt gctggcaaaa
      361 cgtatgccgg gcgatctctc tgaatactcc gttattcaga ccaaagaacc actggatcgt
      421 gaaggtaaag tcagccgcat tgttgaattt atcgaaaaac cggatcagcc gcagacgctg
      481 gactcagaca tcatggccgt tggtcgctat gtgctttctg ccgatatttg gccggaactt
      541 gaacgcactc agcctggtgc atggggacgt atccagctga ctgatgccat tgccgaactg
      601 gcgaaaaaac agtctgttga cgccatgctg atgactggtg acagctacga ctgcggtaaa
      661 aaaatgggct atatgcaggc gtttgtgaag tatggactgc gtaacctaaa agaaggggcg
      721 aagttccgta aagggattga gaagctgtta agcgaataat gaaaatctga ccggatgtaa
      781 cggttgataa gaaaattata acggcagtga agattcgtgg tgaaagtaat ttattgcgaa
      841 tcttcactgc cgttgtttta tataaaaatc cagaataaca acaagttagc aataggattt
      901 tagtcaaagt tgtccaggat tttccttgtt tttgagttca tttgataaga gaattagcgc
      961 ataagtttta gatgctttgc tatgcgttgt agagcacatt gttggtgttt tgtaaggtag
     1021 tactctggtt gctgtaatgc caggggcggt agcgtatttt ttgattatgt gcgattaaat
     1081 taaaatcgta tatcatttag agttatatct gttctggcac tcaactccat ttttggagta
     1141 agattatata ggttatttcg atatcttgat aacagactta atagattaag gggtcttatg
     1201 aaaaatattg cagtaattct tgcatcaggc actggaagtc ggtttggggc taaaatacct
     1261 aaacaattcg ttaaacttgc aggaaaacct gtaattcaat atactctaca tgccttccaa
     1321 aatgcaaaga aaattgatga aattatagtt gttacaaatg aagaatatgt ggactttgtg
     1381 tctgctttga ctatggaaca taactacttt aaggttacga aaataatcca tggtggtaag
     1441 gaacgctatg aatcttcgtg ggcagcgtta caaagtatag aattagatga atgtaatgtg
     1501 atatttcatg acgccgtgcg accgtttgtt tctcaaaaaa tcatcgatga ctgtattgat
     1561 ggtcttaata attacaatgc aattgacgtg gtcgtagatc caaccgatac gattgtaaaa
     1621 atcaaaaata aagagattat gtctattcct gacagaaggt ttttagctag agggcaaact
     1681 ccacaaggtt ttaaaaaatc attaattgaa aaagcatata ttgatttttt aaaggaagaa
     1741 aaaaaagttg cttctgatga ttgtggtatt ttcctcaaat acatgccaaa cgaacccata
     1801 gggattatta aaggcgatga atctaatttt aaaataacgc accagcaaga catttatctt
     1861 gcggataatt taattaagga tggtttgctg gagttaatat ctgagaataa tgattcaatt
     1921 gcgcgaacga tatcagaaaa agtcatagtt atatttggag ggaatagtgg tatagggaaa
     1981 actcttttag taaaagctaa atctcttgac ggaaatgttt attcattcag tcgtagcaat
     2041 ggaattgaca tcagtaatgc catgaacgta agaaaggcat tacagtctgt attcgaaaaa
     2101 gaaggtcgaa ttgattatat aataaataca gctgcagttt tgataaaaaa accaattaat
     2161 tttatgtcag atgaggatat agaacaaagc tgtaatacaa actatttagg ggcaattaat
     2221 atagctagag aaggctatgg ttatttaaag aagacaaaag ggatgttaat taactttact
     2281 tctagctcat ttacacgtgg aagagcttca tatagtcttt attcatcatc taaagcagct
     2341 gtggtgaatt taacacaggc cttatcagaa gagtggttaa atttgggaat taaagttaac
     2401 tgtataaatc cagagcgtac agatactcct atgagaagag tcaattttgg tattgaacca
     2461 ccagaatcct tactaacctc agaacaggtt gcggacatga ctttatcaat tatgtcctct
     2521 gattatacag ggcaagtagt agctgtgaaa aaataacttc atgtcattgc gaaaaaatat
     2581 tagcggctta gctatagctc aaatatttag ctatatagca ccattactgc aattccctta
     2641 tctaagtagg attgtagggg attcatattt aggtgctatt atttttattc tctcaatatc
     2701 acaaatggca atggtgttga cagattatgg gtttgattta tcgattggag aacagatagc
     2761 aagagggaaa agaaataata aatggttaag cgttttttat tgtcaaactg gagtaattaa
     2821 atcatttcta ttagtggtag cattcgtatt tattataagc gtgattcgcc tgtcaaaaag
     2881 tgatattcct gagcaactga taattggatt aatgatttgt gttacattaa atgcttataa
     2941 tccatattgg atatttcagg gccttgaaaa gatatatata tatagtgcat tagtaatcat
     3001 ttcaagatta atagggcttg ttttaatcta tttgctaatt aggactccag atgattatgt
     3061 atattactct tggattttgg catttcaagc ggggatgaca acgataatca gccatattat
     3121 tatatccaga tggggggttc gtgtttgtcg gataaaagta aaaggtataa ttaagcaatt
     3181 taaatatagt tttgactttt ttttatcaag agcttgtgtt tcattatact catctggttg
     3241 cagtgttttc ttagcaacat ttggtgggag tctgaatcaa gttgcgatct atggcgttgc
     3301 agaacagtta tataaagcgg gagtacaggt atttagcccc gttatttcag cgctaactcc
     3361 ttacatggta agaacaaaaa actatattat tttttacaaa attatagtgg tatgcataac
     3421 tgtcacagtg ataggtatgt gtgtcgggtt tttatttggt gctcaaatca ttacattgat
     3481 ttatggtaat ggatatcagg aatccaaatc tgtactagat gtctttatgg ttgtaattct
     3541 tgcaagtgta tttggtatgc ttttaggata tcctgcattg cttcccttag gattgggtaa
     3601 ggttgctaat tatagtgtca ttgcttctgg tatattgcaa ataataatgt tctgtatttt
     3661 gtatattggt ttatttccta ttacagctat tagtgtatgt ctgacatatc taatttgtga
     3721 ttggtttatg ttctttattc gtctttataa ctttatgaaa tataggaaaa ttaatggata
     3781 atcagttacg tgcagcccaa atgaggatgc tggcaattct taacgaagtt gatcgaatat
     3841 gcagactgaa taaaataaat tattggctag atgcagggac cttgttaggt gccaaaagac
     3901 atggtggttt tattccttgg gatgatgata tagatatttg tatgttaagg gatgattata
     3961 ataaatttat tatggcatgt gacaaacaac ttaataaaga gatctttttt cttcaaacag
     4021 cttattcaga tgaatattat gtggacttta atacaccatg taaaataaga atgaataata
     4081 cggagattat agaaaagagt gaagtgaaat ataaatacta cgatgaaaga tctcatcatg
     4141 gtttgtttct tgatatattc ccctatgata aatattcatc taataaatat attaggaagt
     4201 atgttgaacg gtttatatct caactatgta aacttaaagt attatcaaaa tactctggat
     4261 ttcctattac aaaaaaaata cttataaaat gtttaagtag tatcatttgt aaaagacgat
     4321 tattagcttt tgtagattta gttagttttc ggatgagtga gaggaaacat gattatttac
     4381 taggcgctgg aattgaaaca ccatctcata gagcctactt ttctgaggat acaatctttc
     4441 cattaagtga aataaaattt ggaggaagga tgtacttatg cccaaataat gttgataagt
     4501 atctctgtat gatgtttggg gacaattata tgactattcc acctgcagct cagagggtgt
     4561 ggcactacca tagcattaat attgggaaat agactactat ggttgaaggg ttcgttattt
     4621 acaatagcat agtcttgctt gctttattat tttgttattt gaagagattt tttttacccc
     4681 gttactttac tgtctgctta tttttggttc tttgggttcc attagcgctt aggtatggta
     4741 tagggcgtga ttattttagc tatattgaaa tttataaaaa tgttctagtt gcaagagatg
     4801 gtattgagct gggtttttat tatataaatt atatacttgc ttattttaat tttcactatc
     4861 aatatttatt tgcactgtca tccttcatta cagtttattt ttgtgtaaaa agctttgata
     4921 gagattatac gttagtatct attatattat tttgtgcttt aatttacttg caggcattca
     4981 gtattattag acaaatgatt gcagtgtcac tctgtctcta tagttgctca ttatggaata
     5041 aaggttgtaa atgtaaatca tcagtattcc ttatattggc acctttattt catttctctg
     5101 caataataat attactttta gctatagcaa gccgatatat aaaaataaat acgacaagat
     5161 gttattttgt atttgttttt gctcttgtat tcatatttat attgaatggg gtagatttta
     5221 tattttcaaa cgctatatta ttagatagta agtatggata ctatgtaaca tcagcattta
     5281 atcgacctac agaaattggc tcaggaatag gtgttgctat agcattattt ctaccgttca
     5341 tggtatttat aaaatcaact cagttatatg agtataataa aaactataat tttatgctgc
     5401 ttataaatct agtatatgtt atttcttata cactttctct gaaaatatat atatttgcac
     5461 gatttacaga tgctctatct tttgttcaga tattattgat accagtaata cttaaaatct
     5521 cttcccctaa agggatttat tataatttaa tgatggctgt tattttgctt catattttat
     5581 tatttgaagc aaaccttaat gcgaatacaa tttctgcagg aaatatttcg aatagcggat
     5641 taggcatcat gccatataaa agcatactaa atactaaaat agtaggtata tatgactagt
     5701 aaaatattgt ttataactcc attgcctcca ccagttactg gacaatcttt agcttgtatc
     5761 gagttatata attatctaga taaagataat aattgtatta ttgatgtaat aaatctttca
     5821 aaacagtcct ttatatcagg gaaaggctcg ttcaaaagag ttgctgaaat agctggtgtg
     5881 ttattacgtt tattatttct tagacgagat tatgatttaa tttactttac accagctgaa
     5941 tctaaaatgg gaagttataa agatcaattg atatatcttt ttatgtttgc aaagctaaag
     6001 aaaatgtata ttcacttgca tggcggtgca ggcatgaaaa aattattaga aaaagataaa
     6061 tcattaatct tcaaattaaa taaatatttc atctctaggc ttgctggagt gatagtgctg
     6121 ggtgaaagat taaagagtat ttataatgga attattgaaa gtgataaaat ttatgcagtt
     6181 gagaactttt ctcaagatta tttatttata aggcaaagtg agttaaaatt aaaatttaat
     6241 aatcggaaca aaataaaaat actttttctt agtaatttgt tgccaggaaa aggatatatt
     6301 gaattgttaa aatctgcaga ttttttagat gattcttgtg tggaattcca tttcgctggt
     6361 ggtttcgagg atgaacaagc aaaaaaaatt tttttagagg ctattaattt aaagaacaat
     6421 attttctatc atgggattgt cagtggcgat gaaaaacgga atctactaat taaatctgat
     6481 attttttgtt taccaacata ttatccttat gaggggcaac ctataagcat acttgaggca
     6541 tatgctgctg ggtgtgttgt tcttactacc aaccacagtg gtatatttga tgtgtttgaa
     6601 ccttggcgta atggaataga agttgataaa aaaagcacat ctgatttagt aaatgcaata
     6661 aattatttta attcgaacag agaagagatt cgtaaaattg ccatcaataa tattgttgat
     6721 gcaagaaaaa aatatagact taaacatcat ttagaaaaga tgatgacagt attaatgaat
     6781 catgaggtaa tacagcaatg aaaaaatata agatatgcaa tagatgtgtt atggatacat
     6841 ctgatccaaa catttcattt gacgataatg gccagtgtga ttattgttca aactttgata
     6901 aaaatatttt accaaattgg aatgctaata attcaggctt atcaaaattg cttgaaatgt
     6961 cagaatcaat taaaaaagac agtaagggaa gtgattttga ttgtattatt ggcttatcag
     7021 gaggtttaga tagctcttat gcagcgtata ttgcgaaaga aaaaatggga ctacgcccat
     7081 tattatttca tgttgatgct ggatggaata ctgatcaggc agttggtaat attgaaaagc
     7141 ttatcgaagg acttggttta gatctttaca cagaagttat aaactgggaa gaaatgaaag
     7201 atcttcaact gtctttctta aaatcaggaa ttccagatca agatttagtt caggatgctg
     7261 catttttttc atctttatat aagtttgctc ggaaacataa aattaaacat gttattactg
     7321 gttcaaattt ttctacagag tgctgtcgcg aaccagaaga atggggaggg tatttaggta
     7381 ttgatactat gttatttaat gatatccaca gacagtttgg aacccggcca ttaaattcat
     7441 tccccttagt agatatactc atctataagg ttttttatca gaaaatcctt ggaatgaaag
     7501 tacatcatcc attgaattta gtaccattta ctaagaagga tgccgaaaat gaacttagtg
     7561 aaaaatttgg ctggcaacct tttcaacata agcaccatga gtcccgtttt actcgtttct
     7621 atgaagatta ttggttacca cgtaggtttg gttacgaaaa acgtagagct catttctcaa
     7681 gtctaattat gacagggcaa atgactcgcg aacaagctct tgagaggctt ttaaagccag
     7741 aaatggatga acattttctt gaacaagagt ttgagtatat tgcacacaaa ctaggcatta
     7801 gtgttgacga tttaaaagaa ctatttagta ttcccaaaaa aacctacaag gattataaaa
     7861 ataagcgttg gttgattagt ttaggtacaa atatcctacg aacacttgga ttggaaaaaa
     7921 ggtatttcag atgatcaaaa taattgatta tggtattgga aatatacaag cattccttaa
     7981 tgtatataag cgattaggta ttaattcagg tattgctcat tctatcgatg atattaaaag
     8041 tgctacacat ttgatattac ctggagttgg agcttttgac caagctatga cactttttaa
     8101 taactcaggt ttacgagaaa gtattgaaaa acgtattttt gaggatcggg ttccaattat
     8161 tggtatctgt gtaggtatgc agatgcttgc tacctctagt gaggagggga gtttgcctgg
     8221 tttaggatgg attcctggta cggttcgagc attcagcagt aatatctctt caagaagcct
     8281 cccgatgcct catatgggat ggaatagttt aattaaaaaa gtaaatactc cattactgaa
     8341 agattttaaa gaagagtctt ccttctattt tttgcactca ttctattacg aatgtgatga
     8401 cgccggtgat gtattagcaa cagctgaata tggtcataat tttcattgtg tcattggcag
     8461 gaataatata tatggcattc aatgtcatcc ggagaaaagt cattcatcag gttatcaact
     8521 cttaaaaaat tttgcagaga tccaatataa tgcttagacc aagaatcata ccctgtttac
     8581 ttgtccacga ttctggctta gtcaaaacag ttaatttcaa atcaccgaag tatgttggtg
     8641 atccaattaa tgccgttaaa atttttaatg agaaagaagc tgatgaattg atggtccttg
     8701 atattgatgc aacttcaagg ggacttgaac ctaattatgc tctaataaaa aaactcgcag
     8761 cggaatgccg aatgccatta tgctatggag gaggtgtaac tagtgtagca caggctgtaa
     8821 aaattatctc tcttggtgtt gaaaaggtat cgataagttc agcggccgtt gaaaatccaa
     8881 atattgtaaa agatttggca gaagccgttg gtaaacaaag tgttgtagtt gtgctagatg
     8941 ttattaaacg taaaggtctt ttttcaaagg gttatgagct ttcgactagg aataacacgc
     9001 gaacgtataa aatcgatcct atctcgtttg ctcaaaaaat ggttgctttg ggtgcaggcg
     9061 aaatcgtcat caattttgta gataatgatg gtgttatggg gggatatgat gttgaatatt
     9121 cttcgactat caaatcgcaa ataaacgtac ctgttacatt tctcggtggt gcgggaaact
     9181 atgaacatct ctctgcattg attgaccaat gtggcatcgt tggtgcagca gctggtagtc
     9241 tttttgtatt taaaggtaag tatagagctg tgttgataag ctaccctaca cctgagcaga
     9301 aagatattat ttgcaataat ttaaagaaga attattaata aggggttatg atgttcaaag
     9361 ataaaatact gttaattact ggtggtacag gttcatttgg taatgctgta ttaaaccgtt
     9421 ttctttctac agatataaaa gaaattcgta ttttttctcg cgatgaaaag aaacaagatg
     9481 atatgcggaa aaaatataat aatcagaagc tgaaatttta tattggtgat gtcagagatt
     9541 accgtagtat tttgaatgcg actcgcggtg ttgattttat atatcatgca gcggcactta
     9601 agcaagttcc atcatgtgaa tttcatccta tggaagccgt taaaactaat atccttggta
     9661 cggaaaatgt tcttgaagca gctatagcga atgaagtgaa gagggttgta tgcctaagta
     9721 ctgataaagc tgtatacccg attaacgcaa tgggtatttc aaaagctatg atggaaaagg
     9781 tcatggtcgc gaaatcccgt aatgttgatc gcaataaaac agtaatatgt ggtacccgtt
     9841 atgggaatgt tatggcatct cgcggttcag ttattccatt atttgttgat cttattagag
     9901 cgggcaagcc actcacaata actgatccta atatgacccg ctttatgatg actcttgagg
     9961 atgcggtaga tttagttctt tatgcgtttg aacatggtaa taatggtgat atctttgtgc
    10021 aaaaagcacc tgcagcaact attgacacat tagctattgc tttaaaggaa ctactaaatg
    10081 ttccagacca tccggtaaat gtcattggaa cgcgtcatgg cgagaaatta tatgaagctc
    10141 tacttagtcg tgaggaaatg atcgctgcta tagatatggg cgattattac cgtgtcccgc
    10201 cagatcttcg ggaccttaat tatggaaaat atgttgagca aggtgatagc cgaatatctg
    10261 aaatagaaga ttataactct cataatactc aacggttaga tgttgaaggc atgaaagagc
    10321 tcttgctaaa attagccttt attcgagcaa ttcgtgctgg tgaaaaatat aatctggatt
    10381 catgatatga gaatattagt tactggtgca aatggtttta ttggtcgtaa tttatgtttg
    10441 aggcttgagg aacttggtta taaagatctt attagaattg atcgagaatc aacgaagcaa
    10501 gatcttgaac aaggcttaca ggatgccgat tttatttatc acttagctgg tatcaataga
    10561 cctaagactg atgatgagtt tatttctgga aacagtgatt taacaaagca tatagttgag
    10621 tatctccttt ctattggtaa gaatacatca attatgctaa gttcttcgat acaagctgaa
    10681 cttaataatg cttatggggt tagcaaagct gtagctgaaa gctatgtcga aaaatatgct
    10741 gctgctagtg gttcttcgta ttatattttc agatatccaa acgtttttgg taaatggtgt
    10801 aagccaaact ataattcttt tatagcaact ttttgctaca atatttccaa tgatattgag
    10861 attactatca atgatgcatc agcgccagtt aatctggtgt atattgatga tgtttgtact
    10921 gatgctatag ctcttctctc tgggacggtt gaaagcggat ataaagttgt tgcaccaatt
    10981 tattcaacaa cagttggtga ggttgcagaa ttaatttata gcttcaaaaa tagccgttcc
    11041 accctgatca cagaagctgt cgggacggga tttacccgtg cattgtattc tacatggctg
    11101 agttatttac cagcagagaa gtttgcgtac aaggtacctt tttatgggga tgcccgcgga
    11161 gtcttttgtg agatgttgaa aacgccttca gcggggcagt tttcattttt tactgctcac
    11221 cctggtatta cgcgtggcgg acattaccat cacagtaaaa atgagaagtt tttggtcatt
    11281 cgaggtcagg catgctttaa atttgaacat gtgattaccg gtgagcgata tgaactgaaa
    11341 gtttcatcgg gtgagtttaa gatcgttgaa acagttcctg gttggacaca tgacattaca
    11401 aatattggaa ctgaggaatt aatagtcatg ctctgggcaa atgaaatttt caaccgtgat
    11461 gagcccgata ctattgcgag gcctctataa tgaaaaaatt aaaagttatg tctgttgttg
    11521 gaacccgtcc tgagattatc cgtttgtcga gggttcttgc taagtttgat gaatactgcg
    11581 agcatattat tgtccatact ggtcaaaatt atgattacga attaaatgag gtgttcttca
    11641 atgacttggg tgttcgaaaa cctgattatt ttttaaatgc agcgggtaaa aatgcggcgg
    11701 aaaccattgg ccaggttatt attaaggtag atgaagtatt agaaatcgaa aaacctgaag
    11761 caatactggt attgggcgat acgaattcat gtatttctgc cattccggcc aaacgccgta
    11821 aagtgcctat atttcatatg gaagcaggta accgttgttt cgatcaacgc gtgcctgaag
    11881 aaaccaatag acgtattgtt gaccatacgg ctgatatcaa tatgacctac agtgatattg
    11941 ctcgtgaata tctcttggct gaaggtatcc cagctgatcg gatcataaaa actggtagcc
    12001 ctatgtatga ggttctttca tattatatgc cccaaattga tggttcagat gtgctatcgc
    12061 gtttgaatct acagtctggt gagttttttg tagtaagtgc gcatcgtgaa gagaatgttg
    12121 attctccaaa acagctcgta aagcttgcga acattctaaa tactgttgct gaaaaatata
    12181 atcttccagt tattgtctcc acacacccaa ggacacgtaa ccgaatccgt gagcaaggaa
    12241 ttgaatttca ttcaaatata aatttactga aaccattggg tttccatgat tataaccact
    12301 tgcagaagaa ctcacgagct gtgctttcag atagcggtac tatcactgaa gagtcatcca
    12361 tcatgaattt cccagcggta aacatccggg aagcgcatga gcgtccggaa ggctttgagg
    12421 aagcatccgt catgatggtg gggttagagt gtgaacgcgt attacaagcg ctggatattc
    12481 tggcaacaca accgcgaggt gaagtccgtc ttttacgtca ggttagtgat tacagcatgc
    12541 caaatgtgtc ggataaagtt gtcagaattg ttcactctta cacagattat gttaagagag
    12601 tcgtctggaa agaatattga tgaaacttgc tttaatcata gatgattacc tgcccaacag
    12661 tactcgtgtt ggtgcaaaaa tgtttcatga acttgctcaa gaatttatcc agcgtgggca
    12721 cgatgttacg gtaattactc ctggtacggg catgcaagaa gagatttctt ttgatacctt
    12781 tcagggggta aaaacatggc gttttaaaag cgggccgctc aaggatgtaa gtaaaattca
    12841 gcgagcggtc aatgaaacgc ttttgtccta tcgggcgtgg aaagccatca aaaaatgggt
    12901 aaaaaaagag acctttgagg gtgtgattta ttattcacct tccatattct gggggcattt
    12961 agttaaaaaa attaaagctc gttgccaatg tcctgcttat cttattttaa gagatatgtt
    13021 tccacaatgg gtaattgatg caggaatgct taatgctggt tccccaatag aacgctactt
    13081 tcgtcttttt gaaaaaatat cttatcgtca ggcaaatcgt attggactta tgtctgataa
    13141 gaatcttgat gtttttcgga aagataataa aggctatccg tgcgaagttt tgcgtaactg
    13201 ggcatcccta acaccaacga tcatacccaa ggattatata ccactacgta agcgacttgg
    13261 cctagaggat aaaaccattt tcttctatgg tggaaacata ggtcatgcac aggacatgac
    13321 aaacttgatg cgacttgtga gaaacatggc agtatatcct caagctcatt tcctatttat
    13381 tggccagggt gatgaagttg aattaattaa ttcattagcc tctgagtggg cattgacgaa
    13441 tttcacctat ttgccctcgg ttaatcagga tgaatttaag ttcattttgt cggaaatgga
    13501 tatcggcttg ttttctcttt ccgctagaca ctcttcccat aattttcctg gtaagttatt
    13561 aggctatatg gttcagtcgc tacctatttt aggtagcgta aatgccggaa atgatttgct
    13621 cgacattgtc aatcaaaata atgccggatt aatccatgtc aatggtgagg acgataaatt
    13681 atgtcaatct gcgctattaa tgttgcatga tattgatgtg cgccggcaac ttggtttggg
    13741 agcgaatata ttgttgaaag aacaattctc cgttgagtct gcggcacaga cgatagaaat
    13801 gaggttggag gcatgcaatg cgattaattg ataatgacca actcgacaaa ttatatgatc
    13861 aagccgggca atcggaacgt ttacgttccc accttatgat gcacggctcg catcaagaaa
    13921 aggtacagcg tttacttatt gcattagtaa agggcagcta tgttgaaccg cattatcacg
    13981 cacttcctca tcagtgggaa atgttcatag ttatggaggg gcaacttcag gtttgtttgt
    14041 atggtagaaa tggtgaggtt ataaagcaat ttatagcagg agataatact gggataagca
    14101 ttgtggagtt ttctccgggc gatatacaca gtgtcgaatg cctatctcct cgtgctctta
    14161 tggtggaagt taaagagggg ccgtttgact cttcttttgc aaaattgttc gtgtgatgct
    14221 cgtctaaaat gcatcttctg ctatctaccc aagctaaacc tgagttaaca tccataccat
    14281 atttcaagcc gcgaatatct tgcgcggtga ccacccctga caggagtata caatgtcaaa
    14341 gcaacagatc ggcgtcgttg gtatggctgt gatgggacgc aatcttgcgc tcaacatcga
    14401 aagccgtggt tataccgtct ctattttcaa ccgttcccgt gaaaaaacgg aagaagtgat
    14461 tgctgaaaac ccaggcaaga aactggttcc ttactatacg gtgaaagagt tcgttgaatc
    14521 tctggaaacg cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc
    14581 tattgattcc ctcaagccgt acctcgataa aggtgacatc atcattgatg gtggtaacac
    14641 cttcttccag gacaccattc gtcgtaaccg tgagctttct gcagaaggct ttaacttcat
    14701 tggtaccggt gtttccggcg gtgaagaggg ggcgctgaaa gggccgtcca tcatgcctgg
    14761 tggccagaaa gaagcctatg aactggttgc accgatcctg accaaaatcg ccgcagtagc
    14821 tgaagacggg gagccatgcg ttacctatat tggtgccgat ggtgcaggtc actatgtgaa
    14881 gatggttcac aacggtattg aatatggtga tatgcaactg attgctgaag cctattctct
    14941 gcttaaaggt ggcctgaacc tcaccaacga agaactggcg cagaccttta ccgagtggaa
    15001 taacggtgaa ctgagcagct acctgatcga catcactaaa gacatcttca ctaaaaaaga
    15061 tgaagacggt aactacctgg ttgatgtgat tctggatgaa gcagcaaaca aaggtaccgg
    15121 taaatggact agccagagcg cgctggatct cggcgaaccg ctgtcgctga ttaccgagtc
    15181 tgtgtttgca cgttatatct cttctctgaa agatcagcgc gttgccgcgt ctaaagttct
    15241 cactggtccg caagctcagt cagcaggcga caaggctgag ttcatcgaaa aagttcgccg
    15301 tgcgctgtat ctgggcaaaa tcgtttctta cgctcagggc ttctctcagc tacgcgccgc
    15361 gtctgaagag tacaattggg atctgaacta cggcgaaatc gcgaagattt tccgtgctgg
    15421 ttgcatcatc cgtgcgcagt tcctgcagaa aatcaccgat gcatatgccg aaaatccgca
    15481 gatcgctaac ctgctgctgg ctccgtactt caagcaaatt gccgatgact accagcaggc
    15541 gctgcgtgat gtcgttgctt atgcggtaca gaacggtatc ccggttccga ccttcgccgc
    15601 tgcggttgcc tattatgaca gctaccgtgc cgctgttctg cctgctaacc tgatccaggc
    15661 ccagcgcgac tag
//
