LOCUS       EU294170               11575 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serotype O152 O antigen gene cluster, complete
            sequence.
ACCESSION   EU294170
VERSION     EU294170.1  GI:168481326
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O152"
                     /db_xref="taxon:562"
     gene            1177..2262
                     /gene="rmlB"
     CDS             1177..2262
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24814.1"
                     /db_xref="GI:168481327"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSIVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDDEEKNNFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KGLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLAICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1177..10828
                     /note="O antigen gene cluster"
     gene            2262..3161
                     /gene="rmlD"
     CDS             2262..3161
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24815.1"
                     /db_xref="GI:168481328"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTSAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAT"
     gene            3219..4097
                     /gene="rmlA"
     CDS             3219..4097
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24816.1"
                     /db_xref="GI:168481329"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFISG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEIAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLIKMIKGY"
     gene            4102..4650
                     /gene="rmlC"
     CDS             4102..4650
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24817.1"
                     /db_xref="GI:168481330"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDPYIGIKWENE
                     YNVKLSDKDKKNPTMKDVFLFM"
     gene            4666..5703
                     /gene="wfgB"
     CDS             4666..5703
                     /gene="wfgB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgB"
                     /protein_id="ACA24818.1"
                     /db_xref="GI:168481331"
                     /translation="MKKNIIAYYLPQYHEVKENNEWWGKGFTEWTALKKAKKYFPSQK
                     IRLPTDFLGYYDLTESRIIEKQFELAEENGVSGFCLWTYWFGNGEKILEKPLTLILEN
                     KLNVRYCVAWANHSWYNKSKGLLLKEQKYLGEKDYTDFFYYLLPHFKSDNYLKKDNKP
                     IVTIFDPNSIPDLFLFISLWNNLAKENGYEGIYFIGDFTSYNSNYVGVFDGYLDSRVM
                     YRKRTFIQKVREKLVRKHRVKFLGPIRYNYEKMISSLWHNQTKDIKEIPIIFSGWDTT
                     IRHGKQGVFYSDFSEHSFEVNVKNAINYNPQQDIVFLKSWNEWAEGNTVEPDTIFSDK
                     LLRIISKYNKY"
     gene            5703..6914
                     /gene="wzx"
     CDS             5703..6914
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACA24819.1"
                     /db_xref="GI:168481332"
                     /translation="MRKNIFYLLLVQLSNYIFPLITLPYLVRTLGVDKFGYLMLGQAL
                     IQYFILMTDYGFNFSATKKIATSTSIDERNEVYTETLNAKMILVTLCLFLMLIIINII
                     PMFHSISIVCIILFIGVLGNIFFPVYLFQGMEKMKSIAWVTVLARTLMLLSVFLFVKD
                     NNDINAAALAFSIALIVPGMMSIYLIRKNHIVAYKGFSFSKAFIAIKASTPLFISQIA
                     ITFYTTFNSILIGHVFNARQVGIYSAADKLRGAVQSLFIPIQQVVFPRINVERGNIKE
                     KLKVYGGLFILFSLIICVSIFLIGDKIVILYFGTQYAESANLFKWMSVLIFIVSVAIV
                     FSQWGMITLGREKILTKIYIVGALLHCTYAPFFTKNFGLFGTLTAVILTELTITVVMG
                     VFLYKIIKRHK"
     gene            6917..7909
                     /gene="wfgC"
     CDS             6917..7909
                     /gene="wfgC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgC"
                     /protein_id="ACA24820.1"
                     /db_xref="GI:168481333"
                     /translation="MKSNTIDIVIPWVDSSDPKWQEDYMKFACEQRNVDANIHRYRDS
                     GLLKFWFRGIEKNAPWIRKVHFITYGHVPAWLKQDHPKLHIVKHSDYIPQKYLPTFNS
                     HVIELNLHKIDGLSDKFIYYNDDTFLINPVDENFYFKNGKPRDCAILNAVQPDGLSHV
                     ILNNLSIIHKHFCKKQVIRKNILKWINVKYGAALSRTICLMPWPKFVGIKDTHLPIPY
                     LKSTFDKLWELEPEILNATNRSKFRQNSDINQYLFRYWQLVSGEFECSNTMASSAYLD
                     IGKDSIIKIRKELFCSRNKLVVINDADTLEFDSKTKKIQEEFAKIFPFKSTFEI"
     gene            7922..8995
                     /gene="wzy"
     CDS             7922..8995
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACA24821.1"
                     /db_xref="GI:168481334"
                     /translation="MLFTYASYLCILVFSTSVAFCYEHSKSNFKVLLWSVLFLSLWIP
                     AAIRFGVGADYFRYLSMVESVRLGYISTEVGFYFINYFVVWFDLNPQWSIAISSFIVI
                     FLSLKAIPRNYFSISVFVLVCTFYLPSFSLVRQAIAIAFIAYAVKNYIDGNKKNYIIF
                     ILIGSLFHLSALLLVPIYFIARFRVRSSILFLGILIISLLLFLGSGLNALLNSEFFLQ
                     SKYGYYATSDFIQDAKIGSGVGVFIKMLLPLIFITNAKKILKTNQNLNILLWMSVGCV
                     LANVCSMKIHIFNRLADIFLFVNFMVTSYIINSLEKNVIKQLSSMAIIIVFIVFYCRT
                     IQANYNDDLGGIGISPYKTILSK"
     gene            9000..9761
                     /gene="wfgD"
     CDS             9000..9761
                     /gene="wfgD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgD"
                     /protein_id="ACA24822.1"
                     /db_xref="GI:168481335"
                     /translation="MDDYLVSIIMPSYNAEHTISASISSVLKQTYANWELLVCDDDSS
                     DNTRFKVLEFSDSRIKLLTNEYAKGAAGARNTALKYASGRFIAFLDSDDIWIANKLEM
                     QISMMLKNNISFMYGNYEIINNNSIVGKFVAPQKITYNKLLKNCGIGCLTVVLDRTLL
                     NPFSFPFVHKEDYYLWLSILKDNNISAINCGFICSKYRLSQSSISSNKFKELKRQWDV
                     LGDFVENPLARIYYLLNYIVIGIKKHAFDYKNGKK"
     gene            9758..10828
                     /gene="wfgE"
     CDS             9758..10828
                     /gene="wfgE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgE"
                     /protein_id="ACA24823.1"
                     /db_xref="GI:168481336"
                     /translation="MKKIAFVIADITFVGGIERVNTILANKFISEGYSVEIISLYKTN
                     LKINYELDNHIKISFVNDGSYLGEPGSFGRLKKHIGSQFKLFKAIKKSNADFYIINTF
                     PMAFLSFFSLFSKKKYVVIEHVHYDYYSAIVRALRNLLYRFFYKVICINKNDLYKFSK
                     HLDNVVKISNPLSFSCSKVADLSAKKIMAVGRLEHQKGFDLLIDIFAKVNKSNPGWEL
                     HIYGVGTCEKFLTDKINKHGLNNVKLMGSVDHIQQYYPKYSIFAFSSRFEGFGMVLLE
                     AMECGLPCISFDCPTGPSEILGDGEYGILVENGNLIKFSAELANLMSDEEKKIKFSIL
                     SKSRAQEFNIDKIFDEWRKLIS"
BASE COUNT     3590 a   1887 c   2434 g   3664 t
ORIGIN
        1 atcaaagaaa ttattgtggc tgcagggatc aaagagatta ttgtggctgc agggatcaaa
       61 gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg aaaaccactt cgacacctct
      121 tatgaattag aatctctcct tgagcagcgc gtaaagcgtc aactactggc ggaagtacag
      181 tccatttgcc cgccgggcgt gaccattatg aacgtgcgtc agggcgaacc cttaggttta
      241 ggccactcca ttttgtgtgc acgacccgcc attggtgaca acccatttgt cgtggtattg
      301 ccagacgttg tgatcgacga cgccagcgcc gacccgctgc gctacaacct tgctgccatg
      361 attgcgcgtt tcaatgaaac gggccgtagc caggtgctgg caaaacgtat gccgggtgac
      421 ctctctgaat actccgttat tcagaccaaa gaaccactgg atcgtgaagg taaagtcagc
      481 cgcattgttg aatttatcga aaaaccggat cagccgcaga cgctggactc agagatcatg
      541 gccgttggtc gctatgtgct ttctgcagat atttggccgg aacttgaacg cacgcagcca
      601 ggtgcatggg ggcgtattca gctgactgat gccatcgctg aactggcgaa aaaacagtcc
      661 gttgacgcca tgctgatgac tggagacagc tacgactgtg gtaaaaaaat gggctatatg
      721 caagcgttcg tgaagtatgg gctgcgcaac ctgaaagaag gggcgaagtt ccgtaaaggc
      781 attgagaagc tgttaagcga ataatgaaaa tctgaccgga tgtaacggtt gataagaaaa
      841 ttataacggc agtgaagatt cgtggcgaaa gtaatttgtt gcgaattttc ctgccgttgt
      901 tttatataaa caatcagaat aacaacgagt tagcaatagg attttagtca aagttttcca
      961 ggattttcct tgtttccaga gcggattggt aagacaatta gcgtttgaat ttttcgggtt
     1021 tagcgcgagt gggtaacgct cgtcacatcg taggcatgca tgcagtgctc tggtagctgt
     1081 aaagccaggg gcggtagcgt gcattaatac ctctattaat caaactgaga gccgcttatt
     1141 tcacagcatg ctctgaagta atatggaata aattaagtga aaatacttgt tactggtggc
     1201 gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca ggatagtatt
     1261 gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga tgtttctgat
     1321 tctgaacgct atgtttttga acatgcggat atttgcgatg ctgctgcaat ggcgcggatt
     1381 tttgctctgc atcaaccgga tgcagtgatg cacctggctg ctgaaagcca tgtggatcgt
     1441 tcaattacag gccctgcggc atttattgaa accaatattg ttggtactta tgtccttttg
     1501 gaagccgctc gcaattactg gtcagcgttg gatgatgaag agaaaaataa tttccgcttc
     1561 catcatattt ctactgacga agtctatggt gatctgcctc atcctgacga agtaaataat
     1621 aaagaagaat tacccctctt tactgagacg acagcttacg cgccaagtag cccatattct
     1681 gcttcgaaag catcaagcga tcatttagtc cgcgcttgga aacgtaccta tggtttaccg
     1741 accattgtga ctaattgctc taacaattat ggtccttatc atttcccgga aaaattgatt
     1801 ccattggtta ttctgaatgc tctggaaggt aaaggattac ctatttatgg taaaggggat
     1861 caaattcgcg actggctgta tgtagaggat catgctcgag cgttatatac cgtcgtaacc
     1921 gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa aaacatcgat
     1981 gtagtgctcg ctatttgtga tttgttggat gagattgtcc cgaaagagaa atcttaccgc
     2041 gagcaaatta cttatgttgc cgatcgcccg ggacacgatc gccgttatgc gattgatgct
     2101 gagaagattg gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag cgggattcgg
     2161 aagacagtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa aagtggtgcc
     2221 tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc ctcctttttg
     2281 gcaaaacagg gcaggtaggt tgggagctac agcgtgctct ggcacctctg ggtaatttga
     2341 ttgctcttga tgttcactcc actgattact gtggtgattt tagtaatcct gaaggtgtag
     2401 ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa cgcagccgct cacaccgcag
     2461 tagacaaagc agaatcagaa ccggagtttg cacaattact taacgcgacg agtgtcgaag
     2521 cgatcgcgaa agcagccaat gaagtcggcg cctgggttat tcactactct actgactacg
     2581 tatttccggg aaccggtgaa ataccatggc aggaggcgga tgcaaccgca ccgctgaacg
     2641 tttatggtga aaccaagtta gccggagaaa aagcattaca agagcattgt gcgaagcacc
     2701 ttattttccg taccagctgg gtctatgcag gtaaaggaaa taacttcgcc aaaacaatgt
     2761 tgcgtctggc aaaagagcgt gaagaattag ctgttattaa cgatcagttt ggcgcaccaa
     2821 ctggcgcaga gttgctggct gattgtacgg cacatgctat tcgtgttgca ctgaaaaaac
     2881 cagaagtcgc aggcttgtac catctggtag ccagtggtac cacaacctgg cacgattatg
     2941 ctgcgctggt ttttgaagag gcgcgcaaag caggcattcc ccttgcactc aacaagctca
     3001 acgcagtacc aacatcagcc tatcctacac cagctcgtcg tccacataac tcacgtctta
     3061 atacagaaaa atttcagcag aattttgcgc ttgtattgcc tgactggcag gttggtgtga
     3121 aacgcatgct caacgaatta tttacgacta cagcaactta atagtttttg catcttgttc
     3181 gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt aaaggtatta
     3241 ttttagcggg tggctctggt actcgtcttt atcctgtgac tatggctgtc agtaaacagc
     3301 tattacctat ttatgataag ccgatgatct attacccgct ctctacacta atgttagcgg
     3361 gtattcgcga tattctgatt ataagtacgc cacaggatac tcctcgtttt caacaactgc
     3421 tgggtgacgg gagccagtgg ggcctgaatc ttcagtacaa agtgcaaccg agtcccgatg
     3481 gtcttgcgca ggcatttatc atcggcgaag agttcatcag tggtgatgat tgtgccttgg
     3541 ttctcggaga taatatcttc tacggtcatg acctgccgaa gttaatggac gcagcagtta
     3601 acaaagaaag tggcgcaacg gtatttgctt atcacgttaa tgaccctgaa cgttatggtg
     3661 tcgttgagtt tgataaaaac ggtacggcaa ttagtctgga agaaaaaccg ttacaaccaa
     3721 aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc gaaatagcga
     3781 aaaaccttaa gccttctgcc cgtggtgaac tagaaattac cgatattaac cgtatttata
     3841 tggaacaggg gcgtttatcc gttgccatga tggggcgtgg ttatgcatgg ctggatacgg
     3901 ggacacatca gagtcttatt gaagcaagca acttcattgc caccattgaa gagcgccagg
     3961 gactaaaggt ttcctgccca gaagaaattg cttaccgtaa agggtttatt gatgctgagc
     4021 aggtgaaagc attagctgaa cctctgaaaa aaaatgctta tggtcagtac ctgataaaaa
     4081 tgattaaagg ttattaataa aatgaatgtt attaaaacag aaatcccaga tgtattgatt
     4141 tttgaaccga aagtttttgg tgatgagcgt ggtttctttt ttgagagctt taaccagaag
     4201 gtttttgagg aagctgtagg ccgcaaagtt gaatttgttc aggataacca ttcgaagtct
     4261 agtaaaggcg ttttacgcgg actgcattat cagttagaac cttatgcgca aggaaaattg
     4321 gtacgctgtg ttgttggtga ggtttttgat gttgcggttg atattcgtaa atcgtcccct
     4381 acttttggca aatgggttgg ggtgaatttg tctgctgaga ataagcgcca gttgtggata
     4441 cctgaaggat ttgcgcatgg atttttggtg ctgagtgaga ctgcggagtt tgtttataag
     4501 acaactaatt attatcatcc ggaaagtgac agaggtatta gatgggatga cccctatata
     4561 ggaattaaat gggagaatga gtacaatgta aaattgtcag ataaagataa aaaaaatcca
     4621 acaatgaaag acgttttttt gtttatgtaa gtaataggct tttatatgaa aaaaaatatt
     4681 atcgcatatt atcttcctca atatcatgag gttaaagaaa ataatgagtg gtgggggaaa
     4741 ggatttactg aatggacagc tttaaaaaaa gcaaagaaat atttcccttc tcaaaaaata
     4801 aggttgccta ctgattttct tggatattat gatttgacag aatcacgtat tatcgagaag
     4861 cagtttgaac tagctgaaga aaatggtgta agtgggtttt gtctatggac atattggttc
     4921 ggtaatggag aaaaaatttt agaaaaacct cttactctta ttcttgaaaa caaacttaat
     4981 gttcgatact gtgtggcatg ggcaaaccac tcttggtaca ataaatcaaa agggctactt
     5041 ttaaaagagc aaaaatatct tggggaaaaa gattatactg atttcttcta ttacttactt
     5101 cctcatttta agtcagataa ttatctaaaa aaagataata agccaattgt aacaatcttt
     5161 gatcctaatt ccataccaga tttgtttttg tttatatccc tttggaataa tcttgcaaaa
     5221 gagaacggat acgagggtat ctattttatc ggggatttca catcgtataa ttcaaattat
     5281 gtaggagtat ttgatggtta tttggattct agagtaatgt acaggaagag aacctttatt
     5341 caaaaggtga gggagaaatt agttcgaaag catagagtga aatttttggg gcctataagg
     5401 tataattatg aaaaaatgat aagctcatta tggcacaatc agacaaagga tataaaagag
     5461 attccaatta tattttctgg atgggataca acgatacgac atggaaaaca gggggttttt
     5521 tatagtgatt tctctgagca ttcgtttgag gttaatgtta aaaatgctat aaattataac
     5581 ccacaacaag atattgtttt cttaaaatca tggaatgaat gggctgaggg aaatacagtt
     5641 gaaccagaca ctatattctc agataaatta cttagaataa tttcaaaata caataaatac
     5701 tgatgagaaa aaatatattt tatttactgt tggtgcagtt atctaactat attttcccac
     5761 tgattacatt accatatctt gtcagaactt taggagtaga taaatttgga tatcttatgc
     5821 ttggtcaagc attgattcaa tactttattt tgatgactga ttacggtttt aatttcagtg
     5881 caacaaaaaa aatagctaca tcaacaagta ttgatgaaag aaatgaagtc tacacagaga
     5941 cattaaatgc caaaatgata ttggtgacat tatgtttatt cttgatgtta atcattatta
     6001 atatcatacc aatgtttcac tctatatcta tagtttgcat aatacttttt attggggttc
     6061 tgggtaatat tttttttcca gtctatctgt ttcaaggtat ggagaaaatg aaaagtattg
     6121 catgggttac tgtactagca aggaccctga tgttgttatc tgtctttctg tttgtaaaag
     6181 ataataatga tatcaatgct gctgcactag ctttttctat tgcgcttatc gttccaggga
     6241 tgatgtcaat ttatctaata cgaaaaaacc acattgttgc ttataaaggg ttttcttttt
     6301 caaaagcctt tatcgctatt aaagcaagta cacctttgtt tatttctcaa atcgctataa
     6361 cattttatac tacttttaat tcgatactta tagggcatgt atttaatgca aggcaagtag
     6421 gtatttattc tgcagcagac aagttaagag gcgcggttca atctttattt attcctatac
     6481 aacaggttgt gtttccgaga ataaatgttg agcgtggaaa tataaaggaa aaactaaagg
     6541 tttacggtgg tctttttatt ttattttcat taattatatg tgtgtcgata tttttaatcg
     6601 gagataagat tgttatttta tacttcggaa ctcagtatgc tgagtcagct aatttattta
     6661 aatggatgtc agtccttatt tttattgtct ctgtcgctat tgtcttttca caatggggaa
     6721 tgataacttt aggtagagaa aaaatattaa caaaaattta tattgtcggt gccttattgc
     6781 attgcacgta tgctcctttc ttcactaaaa atttcgggct ttttggtacg ttaacggcag
     6841 taatattgac agagttaaca attaccgtag ttatgggcgt gtttttgtac aagataatta
     6901 aaaggcataa ataaaaatga aaagcaacac aattgacatt gtaattccat gggtagatag
     6961 ttctgatcct aaatggcaag aggattatat gaagtttgca tgtgaacaaa gaaatgttga
     7021 tgcaaatatt catcgttata gagatagtgg gttattaaaa ttctggtttc gtggcattga
     7081 aaaaaatgcc ccttggatta ggaaagttca ttttattaca tatggacatg ttccggcgtg
     7141 gcttaagcaa gatcacccta aattacatat tgtaaagcac tccgactata taccccaaaa
     7201 atacctacca acatttaata gccatgttat tgaattaaat ttgcataaaa tagatggatt
     7261 gagtgataag ttcatctatt ataatgacga tacatttttg attaatccag tagatgaaaa
     7321 tttttacttc aaaaatggaa agcctcgaga ttgtgcaatc ctgaacgcag ttcaacctga
     7381 tgggctttca catgttattt taaataatct ttcaattatc cataagcatt tttgtaaaaa
     7441 acaggtgatc agaaaaaata ttttaaaatg gataaatgtt aaatatggtg cggcgttaag
     7501 tagaacgata tgtctaatgc cttggccgaa gtttgtaggt atcaaagata ctcatcttcc
     7561 aattccttat cttaagtcaa catttgataa attatgggag ctcgagccag aaatattaaa
     7621 tgccacaaat cggtcaaaat ttagacagaa ttcagatatt aatcaatatc tctttagata
     7681 ttggcagtta gtttcagggg agtttgaatg ctctaataca atggcatctt cagcctattt
     7741 agatataggt aaggatagta ttataaaaat tcgaaaggaa ctattttgtt caagaaataa
     7801 acttgttgtt ataaatgatg ctgatacttt ggaattcgat tccaaaacaa aaaaaataca
     7861 agaagaattt gcaaagatat ttccttttaa atcaacgttt gaaatataat acggtagaaa
     7921 aatgttattt acctacgcct catatttatg tatattggtg ttttctacat cggttgcatt
     7981 ttgttacgaa cattcaaaaa gtaattttaa agtattgcta tggtcagtat tgtttttgtc
     8041 tctttggata cctgcagcaa ttcgttttgg agtaggcgct gattacttcc gatatttaag
     8101 tatggttgaa tcagttaggt tgggatatat ttctactgaa gttggatttt attttataaa
     8161 ttattttgtt gtttggtttg atttaaatcc acaatggtct attgcaatat caagtttcat
     8221 tgtaatcttt ttaagtttga aggcgattcc acgaaattat ttttctattt cagtttttgt
     8281 tttagtatgt accttttatc tgccttcgtt ttctcttgtt agacaagcta ttgctatagc
     8341 tttcatagcg tatgcagtga aaaattatat cgatggtaat aaaaaaaact atattatttt
     8401 tattttgata ggctctctat ttcatctctc ggcattatta cttgtaccca tttattttat
     8461 tgcgcgattt agagtccgta gttcaattct ctttttgggt attttaataa taagtttatt
     8521 gctattcctg ggtagtggat taaatgcact tttgaatagt gagttttttt tacaaagcaa
     8581 atacggttat tacgcaacaa gtgatttcat acaggatgct aaaatcggaa gtggggtagg
     8641 tgtatttatc aaaatgctat tacctttaat ttttataaca aacgcaaaaa aaatactaaa
     8701 aacaaatcag aatcttaata ttctattgtg gatgtctgtt ggatgtgtgc ttgctaatgt
     8761 atgttcaatg aagattcata tttttaatcg tctagcagat atattccttt ttgttaattt
     8821 catggtaacc tcatatatta tcaattcatt agaaaaaaat gttataaaac aattatcttc
     8881 aatggcaatt attattgttt ttattgtgtt ttattgcaga acaattcagg ccaattataa
     8941 tgatgattta ggtggcattg gtatttctcc ctacaaaaca atactttcta agtgatatta
     9001 tggatgatta tttagtatca attattatgc cttcatataa cgcagagcat acgatttctg
     9061 catcaattag ttccgtgtta aagcaaacat atgctaactg ggaattgctc gtttgcgatg
     9121 atgattcaag tgataataca agatttaaag ttttggagtt ttctgattca agaattaaac
     9181 ttttaacaaa tgaatacgca aaaggtgcag caggtgctag aaataccgct ctaaaatatg
     9241 caagcggccg atttatagct ttcttagata gtgatgatat atggatcgct aacaaattag
     9301 agatgcaaat atctatgatg ctaaagaata atatttcttt tatgtatggt aattatgaaa
     9361 ttataaataa taattcaata gttggtaagt ttgtagctcc ccaaaaaatc acgtacaaca
     9421 aattgctaaa aaattgtggt attggttgtt tgactgttgt tttagacagg actttactaa
     9481 atcctttcag ctttcctttc gttcataaag aagattatta tctatggctg agtattctaa
     9541 aagataacaa tattagtgca attaattgcg gctttatttg ttctaaatat agactttcac
     9601 aaagttcaat ttcatctaat aaatttaaag agttgaaaag gcaatgggat gttttggggg
     9661 attttgttga aaacccattg gctcggatat attatttgct aaattatatt gttatcggta
     9721 taaagaaaca tgcttttgac tataaaaacg gtaaaaaatg aaaaaaatag cttttgtaat
     9781 tgctgatatt acatttgttg gcggtattga gagagtaaac acaatacttg ccaataaatt
     9841 tatcagcgaa ggatactctg tagaaataat atctctatat aaaactaatc taaaaattaa
     9901 ttatgagctt gacaatcaca ttaaaattag ttttgttaat gatggctctt atttaggtga
     9961 gcctggaagt tttggacggc tgaagaaaca catagggtca cagtttaaat tatttaaagc
    10021 cattaaaaag tcaaatgctg atttttatat cattaataca ttccctatgg cttttttgtc
    10081 atttttcagt ctcttttcaa aaaaaaagta tgttgttatt gaacatgtac attatgacta
    10141 ttatagtgca attgttagag cattaagaaa tttattgtat agattttttt ataaagtaat
    10201 ctgtattaat aaaaatgact tgtataagtt ttctaaacat ttagataatg ttgttaaaat
    10261 tagtaacccg ctgagttttt cttgttcaaa agtagctgat ttaagcgcaa aaaaaattat
    10321 ggcggttggc aggctagagc atcaaaaggg ttttgattta ttaatagata tttttgcgaa
    10381 agttaacaaa tcaaatcctg gttgggaact ccatatttat ggtgtcggaa cgtgcgaaaa
    10441 atttctcaca gataaaataa ataagcatgg attaaataat gtaaaactga tgggaagtgt
    10501 cgatcatata caacaatatt atcctaaata ttccattttt gcattctcat caaggtttga
    10561 agggttcggg atggtgttat tggaagcaat ggaatgtggt ttgccatgta tttcatttga
    10621 ttgccctacc ggcccatctg aaatattagg ggatggggag tatggaatct tagttgagaa
    10681 tgggaatctt attaaattta gcgcggaatt agcgaattta atgtccgatg aagagaagaa
    10741 aattaaattt tcaattttat caaagagtcg tgcacaagag tttaatatag acaagatttt
    10801 tgacgaatgg agaaagttaa taagttagta cttatagtaa atgactgaaa acgttgtaca
    10861 aataatttat atcaaaaggt tgcttattcg attaatataa ttaaataagt tacctagccc
    10921 tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatgggg
    10981 cgcaaccttg cgctcaacat cgaaagccgt ggttataccg tctctatttt caaccgttcc
    11041 cgtgaaaaga cggaagaagt gattgccgaa aatccaggca agaaactggt tccttactat
    11101 acggtgaaag agtttgttga atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa
    11161 gcaggtgcag gcacggatgc tgctattgat tccctcaaac catatctcga taaaggcgac
    11221 atcatcattg atggtggtaa caccttcttc caggacacca ttcgtcgtaa ccgtgagctt
    11281 tctgccgaag gctttaactt cattggtacc ggtgtctccg gtggtgagga aggcgcgctg
    11341 aaaggtcctt ccattatgcc tggtgggcag aaagaagcct atgaactggt tgcgccgatc
    11401 ctgaccaaaa tcgccgcagt ggctgaagac ggcgagccat gcgttaccta tattggtgcc
    11461 gatggcgcag gtcactatgt gaagatggtt cataacggta ttgaatacgg tgatatgcag
    11521 ctgattgctg aagcctattc tctgcttaaa ggtggcctga acctcaccaa cgaag
//
