LOCUS       AB812063               12998 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 14097.
ACCESSION   AB812063
VERSION     AB812063.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 12998)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="14097"
                     /db_xref="taxon:562"
                     /note="serogroup:O153"
     gene            <1..898
                     /gene="wcaM"
     CDS             <1..898
                     /gene="wcaM"
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01756.1"
                     /translation="ILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDH
                     VIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGK
                     HFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGK
                     YLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKRASLELHNKPQH
                     LFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDI
                     DRINHHIVNVEKINFRLPERRE"
     gene            1056..1304
                     /gene="gne"
                     /pseudogene="unknown"
     CDS             1056..1304
                     /gene="gne"
                     /note="O153_01; UDP-N-acetylglucosamine 4-epimerase"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             1995..3065
                     /note="O153_02"
                     /gene="wetO"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01757.1"
                     /translation="MKKVTFLINSLTQGGAEKIALQLYLNLSNSVNVINFVTLTNEDF
                     YKKEIKKHVINHSNKITKTTKFMSLAKYFRGNNDVVLCFSLDLACYLYILRVLNIFKG
                     EVICRFINNPDNEIGTSLINKIKKRFLFYVLKKCDLVICQSDAMKDLLISKYEFNNNK
                     TVRIYNPISIANNNESSFEQTDKEILKLLFVGRLSEQKNLDDIIRISQLLRDKGILFL
                     WKVVGKGPLENTFKDLIREHNFQESIVMCGSSHDIENYYKWADITTLVSHYEGLPNVL
                     LESISHRTPCVSYDCPTGPSEIIIHEKNGVLVPQYDVDNFYQSLIRVKELNLKNDNIE
                     NTLMKFSEKKVCDEYYKILFKR"
     gene            3071..4300
                     /gene="wzx"
     CDS             3071..4300
                     /gene="wzx"
                     /note="O153_03"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01758.1"
                     /translation="MKKVLGNFLNLTLLQLSTMVAQILTYPYLNRTLGSEIYGEIILQ
                     QIIVFHLQMIVFFGTDLSVVRLAAKYSTSYKKLKILVSKIILGRFYISLIISLSYVIY
                     IYCSGLNDWFYFFVFSIFEAVITLRWFYHGIQQLNKFTLPFCLVRFLGVLLLFIFVNT
                     QDDWYKVIIITVLSSLLSVIVSFYSYYVKYNLTYIKFRQIYIIFSDSFSLFATNIVSV
                     IKDRSGGIFISYFMGTSSLVYYDFCMKIVGVISSITSSISASLYPMFSGNYNHSTFKR
                     YNLLILLFSIIPFIITLLADKYVIFLIDYIFSINIFDIKLLLPVFGFMIFVRSHGYFI
                     GLCYLMALNYKKSYASSLIYSGMFYLFFMSGMIFLGYKNILTLAVGLSLSLLLEYFHR
                     IYKFISTRKNANESHAE"
     gene            4305..5378
                     /gene="wzy"
     CDS             4305..5378
                     /gene="wzy"
                     /note="O153_04"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01759.1"
                     /translation="MAYILAITMVIQSYSKVFFASDTIFQNIFFYALVFFSILIVSIS
                     KFRLIDIFLIITSLIIYLAFGNGFALKLFLVSLAVRCLDINKLLRCYLLLATIAFITV
                     ILFNVGGDNSLIYFKGDGVFRIARETLGFDNPNKPFYYLLPIFCCFVFLYFKRYPISC
                     ITAIIIVTYAVYIKTLTTTGLFSNALLVLMLLIYYFLPNKTNRILGNPLLITCSIIVL
                     YFASFFIAFTYHSDSNVNFFLSHRPEYWYEIIRTTNIYLLIFGQALDLQLVPLDNSYI
                     HSVIYMGAFFCIIMIFCYWLGLTRAKLRGVNISIISILCIYVFFYSFGETLLVEPTLN
                     ITFIIIFNYIRDHNKYESIDLQP"
     CDS             5362..6504
                     /note="O153_05"
                     /gene="wetP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01760.1"
                     /translation="MIFSHEYPPIGGGAGVVANQLIDHFCEDSAIEQIDLLTRFSHQY
                     NINKRVHNIFMVNIHNIIWPLEYFFYLKKTINLSLYDLIICNDSISIYIAGMLFSSRE
                     LEKTVCFLHGSEPEFIYQNNNIQKRLLNLRFFFHRALIKCSFIGAHSEFMKEKFLKNL
                     PKKLTINSDKIIPLYFGYDASLFNTDNKIENKNTIRNKYKIKESDILLLTVSRVEEKK
                     GFVKMLNTFEIMKSMNENLKWMIVGDGGFLESLKEIAFRKGIFDSLIIVGKIPRSELC
                     MFYNSADIFWLLSEYQEAFGLVYIESQACGVPAIGYNNSGVREAVVDGVTGYLINNLD
                     EMLDIVVTKKFQIITNESLYDFSKKFDSKQCYLKILKAFNEKVINE"
     CDS             6497..7336
                     /note="O153_06"
                     /gene="wbbD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01761.1"
                     /translation="MSDVIMHDKKTEEFSVLMSLYCNEKPEFLNQCLISIHEQSVKPN
                     EIIIVYDGYIPAELDNVVIEWSKVLPVKVCKLHTNMGLGDALNFGIKHCNNELIARMD
                     TDDICAKDRFKLQLQYFNENPTLTLIGGGIEEYDEEMQVLRGTRFTKEKHADIVKYAC
                     FKNPFNHMTVMFKKKDIQSVGGYKKHLLMEDYNLWLRLLNNGYKTYNLPEILVYARTG
                     INMVRKRRGMSYFKSEIQLFKLKRMLNCNSYSKNCVIFLIRVLPRFLPVSVLSIVYKI
                     MRK"
     gene            7448..8854
                     /gene="gnd"
     CDS             7448..8854
                     /gene="gnd"
                     /note="O153_07"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01762.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            9103..10269
                     /gene="ugd"
     CDS             9103..10269
                     /gene="ugd"
                     /note="O153_08"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01763.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKELNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAQRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(10335..11339)
                     /note="O153_09"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01764.1"
                     /translation="MKFLVTGAVGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFVDERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            11691..12725
                     /gene="wzz"
     CDS             11691..12725
                     /gene="wzz"
                     /note="O153_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01765.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(12822..>12998)
                     /gene="hisI"
     CDS             complement(12822..>12998)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01766.1"
                     /translation="IAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQDL
                     DLTTVIENLRKRHQ"
BASE COUNT     4021 a   2253 c   2569 g   4155 t
ORIGIN
        1 catcttacgg cagggatttc ataaccagat aatcggtgcc aacatcacca attgtaagtt
       61 cagcgactta cagggggacg ctattgaatg gaacgtggca attaacgaca gtgatatttt
      121 gatctccgac catgtcatcg agcgcatcaa ctgtactaac ggcaaaatca actggggcat
      181 tggcataggc cttgcgggaa gcacttacga taataattac ccggaagatc aggcagtgaa
      241 aaactttgtc gtggcgaata tcacgggatc ggattgtcgg cagttgatcc atgttgaaaa
      301 tggtaaacat tttgttattc gtaatatcaa agcccgcaat atcacgccgg atttcagtaa
      361 gaaagcaggt attgataacg cgacagtcgc tatttacggt tgtgacaatt tcgtgattga
      421 taatattgaa atgattaata gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa
      481 atatctctcg ataccgcaaa atttccgagt gaataatatt caactggata atacccacct
      541 tgcttacaaa ttgcgcggta tccaaatctc tgccgggaat gccgtctcct ttgtggcact
      601 gactaacatt gaggtgaagc gtgcgtcgct ggagttacac aacaaaccgc aacatctttt
      661 tatgcgtaat atcaaagtga tgcaggaatc ctcagttgga cccgcattga gcatgaactt
      721 cgacatgcgc aaagacgttc gcggcgtctt tatggcgaaa aaagaaacac tgctgtctct
      781 tgcaaatgtt catgcggtga atgaaagagg gcaaagctcc gtcgatatcg acaggataaa
      841 tcaccatatt gttaatgtgg aaaagattaa ctttagattg ccggaacgga gggagtagat
      901 ttgcgaccat tcataaactt actggatata atgttctaaa agattataat cctcacacta
      961 actgttaaag ctggggctgg gcagcattga ctactcaggg aaggtactca tctttatctt
     1021 gtgtttctta cgtaacaacc agactaaagt gatttatgaa cgataacgtt ttgctcatag
     1081 gagcttccgg attcgtagga acccgactac ttgaaacggc aattgctgac tttaatatca
     1141 agaacctgga caaacagcag agccactttt atccagaaat cacacagatt ggcgatgttc
     1201 gtgatcaaca ggcactcgac caggcgttag ccggttttga cactgttgta ctactggcag
     1261 cggaacaccg cgatgacgtc agccctactt tcgatttttc ataaagtaaa atcaaatcat
     1321 aacaaatgga gagtctttta ttattttatg gcgaccacta ttactggatt agccctttat
     1381 acgaaaatgt tatctgaata cgtggttttc atatgttgtt tgtatatgct ggtatataga
     1441 gttttatcac ccttattgat tcagaaaaaa aattgactat aagattatat aaataataaa
     1501 gtaccttatg ctaaggagat tgaactgaag aaggtgattt aaaaccttct atactatact
     1561 ttattaaata ctaagataac gcgtaaatgg ttaaaatgac agtgattaaa atgataaata
     1621 tgttatcttt caattaattc ctaatttgaa ttctattcag tacctaaata ttatcttcat
     1681 ttaaattcaa tggatggttt tggaaattta atatgactta ttctgatttc tagaaaaata
     1741 ataagaattg ttattatatc ttggggggat taaagaattc atattagtaa tcaatgagaa
     1801 taaaataaaa gagggaatta atttacgtta ttttgataaa catttttcac aattaaaatt
     1861 tttaaatgcg ttaagtattt atcaatacaa aatataaaag ggttatcccg aaagctactg
     1921 ctcacgttag tgttgaggga atatattttt tactcttaca aagagtgatg aacttcggtt
     1981 aaaagtgaat ataaatgaaa aaagttactt ttcttattaa tagcttaaca caaggtggag
     2041 cagaaaaaat tgcattgcaa ctttacctaa atttgagtaa ttcagttaat gttattaatt
     2101 ttgttacatt gacaaatgaa gatttttata agaaggaaat aaaaaaacat gtgataaatc
     2161 atagcaataa aataactaaa acaacaaaat ttatgtcatt agcaaagtat tttagaggta
     2221 ataatgacgt tgtcttatgt ttttcattag atcttgcttg ttatttatat atccttcgcg
     2281 ttctaaatat ttttaaagga gaggtgattt gtagattcat aaataatcct gacaatgaaa
     2341 taggaacttc attaattaat aaaataaaaa aaagatttct tttttatgtg ttaaaaaaat
     2401 gcgatcttgt tatttgccaa tcagatgcta tgaaggatct tttgataagc aaatatgaat
     2461 tcaataataa taaaacagta aggatttata atccaatatc tattgcaaat aataatgaat
     2521 ctagtttcga gcaaactgat aaagaaattt taaaattact ttttgttgga aggcttagcg
     2581 aacaaaaaaa tttagatgat ataattcgta tttctcaact attgagagat aaaggaatat
     2641 tatttttgtg gaaggttgtc ggaaaagggc cgctggaaaa cacattcaag gatttgataa
     2701 gagaacacaa tttccaagag agtatcgtaa tgtgtggttc gtcacatgat atagaaaact
     2761 actataagtg ggcagatatc acaactttag tttctcatta tgaagggctt ccgaatgtat
     2821 tgttagaatc catctcgcat agaacgccat gcgtaagtta tgactgtccc actggtcctt
     2881 ctgaaataat tattcatgaa aaaaatggtg ttttagtacc tcaatacgat gtggacaatt
     2941 tttatcaatc tcttattcga gttaaagagc taaatttaaa aaatgataat attgagaata
     3001 cattaatgaa attttcggag aaaaaagttt gtgatgaata ctataaaatt ctttttaaga
     3061 gataatcaga ttgaagaaag tattaggaaa tttcttaaat ttgacacttt tacagttgtc
     3121 aacaatggtt gcacaaatcc ttacttaccc ttacttaaat aggactctgg gcagtgagat
     3181 atatggggaa ataattcttc agcagataat agtttttcat cttcaaatga tagttttttt
     3241 tggtacagac ctgtccgtag tgaggttggc agcaaaatat agtacaagct ataaaaaact
     3301 gaaaattttg gtgagtaaaa taatccttgg tcgattttat atttctttaa tcatttcttt
     3361 atcttacgtt atttatattt attgtagcgg tttaaatgat tggttttatt tttttgtatt
     3421 ttctattttt gaagcagtta ttactttgag atggttttat catggtattc agcaacttaa
     3481 taaatttacc ttaccttttt gtttagtcag atttttagga gtgttgcttc tatttatctt
     3541 tgttaatact caggatgatt ggtataaggt tataataata actgtattgt cgtcattact
     3601 cagtgttata gtatctttct attcctacta tgttaaatat aatcttactt atattaagtt
     3661 tcgtcaaata tatattatat tttctgattc attttcgctc tttgctacaa acattgtttc
     3721 ggtgattaag gaccgatctg gaggtatttt tatcagctat tttatgggaa catcttcact
     3781 agtttattac gatttttgca tgaaaattgt tggtgtaatt tcaagtataa cttctagcat
     3841 atcagcttct ttgtacccga tgttctcagg aaattataac cattcaacat tcaaaagata
     3901 taatttacta atattgttgt tttcgataat tccatttatt attactctgc tcgcagataa
     3961 atatgtgatt tttttgatag attatatatt tagtattaat atatttgata ttaaattatt
     4021 gctgccagta tttggattta tgatttttgt aagatctcat ggttacttca taggcctctg
     4081 ttatctaatg gcattaaact ataaaaagag ttatgcttca tcattgattt attcgggtat
     4141 gttttatttg ttttttatga gcggtatgat ttttttaggc tataaaaata ttttaacact
     4201 tgctgtaggg ttatcattat cattgttact agagtatttt catcggatat ataaatttat
     4261 tagtacaagg aaaaatgcca atgaatctca cgctgaataa ttttatggca tatatactcg
     4321 ccattacaat ggtaattcaa tcttatagta aggttttctt tgcatctgat actatctttc
     4381 agaatatctt tttttatgct ttagtgtttt tttcaatatt aattgtaagt atatcaaagt
     4441 tcagattgat tgatatattt ttgattatta catcattaat aatttattta gcattcggta
     4501 acggctttgc attaaagctt ttcttagttt cattagcagt acgttgttta gatataaaca
     4561 aattgctccg ttgttatctc ttactagcga ctattgcatt tataacagtt atattattca
     4621 atgtgggagg tgataactct ttaatttatt ttaaaggaga tggtgtattt agaattgcaa
     4681 gggaaacatt aggttttgat aatcctaaca aaccatttta ttatcttttg ccgatatttt
     4741 gttgttttgt ttttctatat ttcaaacgtt atccaatatc atgcataaca gctattataa
     4801 ttgttacata tgcagtatat ataaaaacac taacaactac aggacttttc agtaatgctt
     4861 tgcttgtttt aatgttgttg atatattatt ttcttcccaa taaaacaaat cgaatattag
     4921 gtaatcctct tttaattact tgctcaatta ttgtattata ttttgctagt ttttttattg
     4981 ccttcactta tcattctgat agtaatgtga atttctttct ttctcacagg ccagaatatt
     5041 ggtatgagat aattagaaca acaaatattt atttgctaat atttggacaa gcattagatc
     5101 ttcaattggt tccgttagat aattcctata tacattctgt catttatatg ggggcgtttt
     5161 tctgtattat aatgattttt tgctattggc tggggttaac cagagctaaa ctcagagggg
     5221 taaacatttc aattataagt atattatgca tatatgtgtt tttttattct tttggtgaga
     5281 ctttgttagt tgagccaaca ctcaacatca catttattat tatatttaac tatatacggg
     5341 atcataataa atatgaaagt attgatcttc agccatgagt atccacctat aggaggaggg
     5401 gctggtgtag tagctaatca actaatcgac catttttgtg aagacagcgc gatcgaacaa
     5461 attgatctgc ttactcgttt ttcgcatcag tacaatatta ataagagagt gcataatatt
     5521 tttatggtta acatccataa tattatatgg cctctagagt acttttttta tcttaagaaa
     5581 actataaatc tttctcttta tgatcttatt atctgtaatg actccatatc tatatatatt
     5641 gcaggtatgc tgttctctag tagagaacta gaaaaaaccg tatgtttttt acatggttct
     5701 gaacctgagt ttatatatca aaataataat atccaaaaaa ggttacttaa tttaaggttt
     5761 ttttttcaca gagctctaat taagtgtagc tttatcggag ctcatagtga atttatgaaa
     5821 gaaaaattcc tgaagaactt gcctaaaaaa ttaactatta attcagataa aataatcccc
     5881 ttatactttg gctatgatgc atcattgttc aatactgata ataaaataga aaataaaaat
     5941 acaattcgta ataaatataa aataaaagaa agtgatatat tattacttac tgtttcaagg
     6001 gtggaagaaa aaaagggatt tgtaaaaatg ttaaatacct ttgaaataat gaaatccatg
     6061 aatgagaatc taaaatggat gatagttggg gatgggggat tccttgaaag tttgaaagaa
     6121 attgcattta ggaaagggat ttttgattca ctcattatcg taggtaaaat tccaaggagt
     6181 gagttgtgca tgttttataa ttctgcagat attttttggt tattatcaga ataccaagag
     6241 gcttttggtt tggtatatat tgaatctcag gcatgtgggg tacctgccat tggctataat
     6301 aattcaggag taagagaggc tgttgttgat ggagttacgg ggtatttaat taataatctt
     6361 gatgaaatgt tggatatcgt agtgacaaag aaattccaaa ttataactaa tgaatcccta
     6421 tatgattttt caaaaaaatt cgactcaaaa caatgttact taaaaatact aaaagcgttt
     6481 aacgagaaag ttatcaatga gtgatgttat aatgcacgat aaaaaaactg aggaattctc
     6541 agtattaatg tctttatatt gtaacgaaaa acctgaattt ttaaatcaat gcttaataag
     6601 tatccatgag caatctgtca agcctaatga gatcatcatt gtttatgatg gctatatacc
     6661 ggctgaatta gataatgtcg taatagagtg gagtaaagtt ttaccagtaa aagtttgtaa
     6721 gttacacaca aatatggggc ttggcgatgc acttaacttt ggtatcaaac attgtaataa
     6781 tgaattgatc gcaagaatgg atactgacga tatttgtgct aaggatagat tcaaattgca
     6841 actgcaatat ttcaatgaaa accctactct tactttaatc gggggaggta ttgaagaata
     6901 tgatgaggaa atgcaagtcc tgagaggaac gcgttttaca aaagaaaaac atgctgatat
     6961 tgtcaaatat gcttgcttca aaaacccatt caatcatatg actgtaatgt ttaagaaaaa
     7021 agatattcag tctgtcggtg gatataaaaa acatctatta atggaagatt ataatctttg
     7081 gttgcgtctt ttaaataatg ggtataaaac atataactta ccagaaatac ttgtttacgc
     7141 gcgaacgggt attaacatgg ttagaaaacg tagaggtatg agttatttta aaagtgaaat
     7201 tcagcttttt aaactgaaaa ggatgttgaa ctgtaattca tatagtaaaa actgtgttat
     7261 ctttttaatt agagtactcc cacgattcct tccggtatct gtactttcaa ttgtatataa
     7321 aataatgcga aagtgacaaa cattttttta taaagagaaa attaattgct tcttcctttt
     7381 ttgaaaacaa taaatattgt atattactgt tcttttagaa ctacttcccc gcagacagga
     7441 gtaaacaatg tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct
     7501 tgcgctcaac atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt cccgtgagaa
     7561 gacggaagaa gtgattgccg aaaatccagg caagaaactg gttccttact atacggtgaa
     7621 agaatttgtt gaatctttgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc
     7681 aggcacggat gctgctattg attcccttaa gccatacctc gataaaggtg acatcattat
     7741 tgatggtggt aataccttct tccaggatac catccgtcgt aaccgtgagc tttctgccga
     7801 aggctttaac ttcattggta ccggtgtttc cggcggtgaa gaaggagcgc tgaaaggtcc
     7861 ttctattatg cctggtgggc agaaagaagc ctatgaactg gttgcgccga tcctgaccaa
     7921 aatcgccgcc gttgctgaag atggcgaacc gtgcgttacc tatattggtg ccgatggcgc
     7981 aggccactat gtgaagatgg ttcacaacgg tatcgaatac ggtgatatgc agctgattgc
     8041 tgaagcctat tctctgctta aaggtggcct gaatctttcc aacgaagaac tggcgcagac
     8101 ctttaccgag tggaataacg gtgaactgag cagctacctg atcgacatca ccaaagatat
     8161 cttcaccaaa aaagatgaag agggtaacta cctggttgat gtgattctgg atgaagcggc
     8221 taacaaaggt accggtaaat ggaccagcca gagtgcgctg gatctcggcg aaccgctgtc
     8281 gctgattacc gagtctgtgt ttgcacgata catctcttct ctgaaagatc agcgcgttgc
     8341 cgcatctaaa gttctctctg gtccgcaagc tcagtcagca ggcgacaagg ctgagttcat
     8401 cgaaaaagtt cgccgtgcac tgtatctggg caaaatcgtt tcttacgccc agggcttctc
     8461 tcagctacgc gctgcgtctg aagagtacaa ctgggatctg aactacggtg aaatcgcgaa
     8521 gattttccgt gctggctgca tcattcgtgc gcagttcctg cagaaaatca ccgatgctta
     8581 tgccgaaaat ccgcagatcg ctaacctgct gctggctccg tacttcaagc aaattgccga
     8641 tgactaccag caggcgctgc gtgatgtcgt cgcttatgcg gtacagaacg gtatcccggt
     8701 tccgaccttc gccgctgcgg ttgcctatta tgacagctac cgttctgctg ttctgcctgc
     8761 gaacctgatc caggcacagc gtgactattt cggtgcgcat acttataagc gcattgataa
     8821 agaaggtgtg ttccataccg aatggctgga ttaatctgat ttaaatcaat taatcaaagc
     8881 aaggcccgga gaaaccctcc gggctttttt attatacaaa gcggcaggtt atggcctttt
     8941 tttataattt atgattaaaa aagcgatata atacagagcc gcacagcagg atcgctgcct
     9001 tgacagttca tctacatcag cgttaaaaat cccgcagtag atgaagctgt ggtggtggat
     9061 taatgaccac tctaaatgtt taaccggaag aagtcagagc taatgaaaat aacaatttca
     9121 ggaacaggtt atgttggtct ttcaaatggt attctgattg cgcaaaacca cgaagtggtt
     9181 gcactggata tcgttcaggc caaagtggac atgcttaaca agaagcagtc accgattgtt
     9241 gataaggaga ttgaagagta tctggcgact aaagagctca atttccgcgc tacgacagat
     9301 aagtatgacg cgtataaaaa tgccgattac gttattattg ccacgcctac cgattatgac
     9361 ccaaaaacaa attattttaa tacctcaagc gtggaagcgg tcatccgtga tgtgacagaa
     9421 attaatcccg acgctgtaat gattataaaa tcaactatcc ctgtcggttt tacagagtcc
     9481 attaaagaac ggtttggtat tgaaaatgtg atcttttcgc cggagttttt acgtgaaggg
     9541 aaagcacttt atgataactt acacccatca cgcattgtga ttggcgagca atccgatcgc
     9601 gctcaacgtt ttgcagcgtt attacaggaa ggcgccatta agcaagatat accaacgttg
     9661 tttactgact caaccgaagc tgaggcgatt aaactttttg ctaatactta tctggcgatg
     9721 cgtgtggctt atttcaatga attagatagt tacgctgaaa gtctggggct taattcccgc
     9781 cagattattg agggtgtatg ccttgacccg cgtatcggta atcactacaa caacccgtca
     9841 ttcggatatg gtggttattg tctgccgaaa gatactaagc agttactggc aaactatcag
     9901 tcagtaccga ataacttgat ctcagcaatt gtcgatgcca atcgcacgcg aaaagacttt
     9961 attgccgatt ctatccttgc acgtaaaccg aaagttgttg gcgtctatcg tctgattatg
    10021 aagagtggtt cggacaattt ccgtgcttca tcgattcagg gtattatgaa gcgaatcaag
    10081 gcgaaaggtg tgccagtaat cgtttatgag ccagcgatga aagaggacga ttttttccgg
    10141 tcgcgcgtgg tacgtgatct ggatgcgttc aaacaagaag ctgatgttat tatttctaac
    10201 cgtatgtctg ccgatctggc tgatgtagca gataaagttt atacgcgcga cttgtttggc
    10261 aatgattaat tattttgttt cattctaaga aaaggcccta atatattagg gccttttctt
    10321 atggttttgt aaaatcaaac tttatagaag ttacgatacc attctacaaa gttctttacc
    10381 ccctctttaa ctgacgtttc aggtttgaat cctattacgt catacagtgc ttttgtatca
    10441 gcactggttt ccagtacatc accgggttgg agaggcatca tattcttgtt ggcttcaata
    10501 cccagagcct cttctaaggc attgatatag tccatcaact ccacaggcga actattacca
    10561 atgttataga cacgatatgg tgctgaactt gttgctggcg agcctgtttc tacagcccag
    10621 tgtggatctt tttctggaat aacatcctgt aagcgaataa tagcttcggc aatatcatca
    10681 atataagtaa agtcacgctt cattttgccg aagttgtaaa catcaatgct tttaccttcc
    10741 agcatggctt tagtgaattt aaataatgcc atatccggac gtccccatgg accataaacc
    10801 gtaaagaaac gcagccctgt ggtcggtaag ctatacaaat gagaatacgt atgagccatg
    10861 agttcattcg cttttttagt tgctgcataa agcgaaacag gatgatctac agagtcatct
    10921 gtagagaaag gcatcttgcg gttcatgcca taaacagaac tggaggaagc gtaaagtaga
    10981 tgctgaacat tattatggcg acatccttct agtatgttca ggaatccaat caggtttgca
    11041 tctgcatatg cattgggatt ttcaagggag taacgtacac cggcttgcgc agcgaggttt
    11101 attacgcgat cgaaccgctc gtctacaaac agtgccgcca ttttctcgcg atcggccagg
    11161 tcaattttat aaaaactgaa attgtcgtgc ttgagtaaat caagtcgtgc ttgtttgagg
    11221 ttgacatcgt aataatcatt taagttgtca atgcctacaa cctgatgacc agctgcaaga
    11281 agccgtttac ttacatagaa accgataaag ccaacagctc ccgtaaccag aaatttcatt
    11341 tataatcctc gctcaggcta gaatatagcc aattttcatc tagcataact gaaagttaaa
    11401 tcataccgtt agacaagaaa aaaagataat cggtatcagt tctaaacctg gctgtttttt
    11461 ctggtaacgt gctcatttta caatcaaagc tgttctaagc tgactataca agccgacgtc
    11521 attatctcca accgtatggc agaagagctt aaggatgtgg cagataaggt ctacacccgc
    11581 gatctctttg gcagtgacta acatcctgtt atcagggcga ttttcgccct gattctctta
    11641 tgttcccttt gtaataattc attattttta tcatttatcc tatagcattc atggcgatta
    11701 tcgctaaact atggcgactt aaaattattt cgtccgttag ggtaatgatg agagtagaaa
    11761 ataataatgt ttctgggcaa aaccatgacc cggaacagat tgatttgatt gatttactgg
    11821 tgcagttgtg gcgcggcaag atgacaatta tcatctctgt cattgtcgct attgccctgg
    11881 ctatagggta tctggccgtt gccaaagaaa agtggacttc cacagctatc gttactcaac
    11941 ctgatgtggg gcaaattgct ggttatacca acgcgatgaa tgtgatctac ggatcggctg
    12001 taccgaaagt ctccgacatt caggcttcac ttatcggacg ttacagcact gctttctcag
    12061 cattagcgga aacgctggat aatcaggaag aacctgaaaa actgaccatt gagcctaccg
    12121 ttaaaaatca gtcattacct ttagcggttt cttatgtggg tgaaacacct gaaggtgcgc
    12181 agaaacagct ggcgcagtat atccagcaag ttgacgacca ggtaaatgaa gaactggaaa
    12241 aagacctgaa agacaacatc gcgctgcgta tgaaaaactt gcaggattct ctgaaaaccc
    12301 aggaagtagt cgcgcaggag cagaaagatc tgcgtatccg tcagattcag gaagcgttgc
    12361 agtatgcgaa tcaggcgcag gtgacaaagc cacaggttca gcagactgaa gatgtgacac
    12421 aagatacgtt gttccttttg gggagcgaag cgctggagtc gatgattaag catgaagcga
    12481 cccgtccgtt ggtgttctca ccaaactact atcagacacg tcaaaacctg ctggatatcg
    12541 aaagcttaaa ggttgatgat cttgatattc atgcttaccg ttatgtgatg aaacccacgt
    12601 tacctattcg tcgcgatagc ccgaaaaagg caattacctt gattctggcg gtgctgctgg
    12661 gaggcatggt tggcgcgggg attgtgctgg ggcgtaatgc tctacgcaat tacaacgcga
    12721 aataatcttt gcagcattaa agaaaacggg cagggtggtg acaccttgcc cgtttttttt
    12781 gccggatgcg acaacaacat agcatccggt cagcacgcaa ctcactgatg ccgtttacgc
    12841 aggttctcaa ttaccgtcgt taaatccaga tcctgatcct gcaacagcac cagcaggtga
    12901 tacatcaaat cagacgcctc gttggtcagt tcaaagcggt catgtaccgt tgctgccagt
    12961 gcggtttcca ccccttcttc gcccactttc tgcgcaat
//
