LOCUS       AB812064               20962 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E1541-68.
ACCESSION   AB812064
VERSION     AB812064.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 20962)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1541-68"
                     /db_xref="taxon:562"
                     /note="serogroup:O154"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01767.1"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1064..2059
                     /gene="gne"
     CDS             1064..2059
                     /gene="gnu"
                     /note="O154_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01768.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     CDS             2177..2485
                     /note="O154_02"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS3"
                     /protein_id="BAQ01769.1"
                     /translation="MTHMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSL
                     YESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK
                     "
     CDS             2482..3348
                     /note="O154_03"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS3"
                     /protein_id="BAQ01770.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRELRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGG
                     LSPEQFENKNLA"
     gene            3563..4456
                     /gene="galF"
     CDS             3563..4456
                     /gene="galF"
                     /note="O154_04"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01771.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDVMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            4825..5910
                     /gene="rmlB"
     CDS             4825..5910
                     /gene="rmlB"
                     /note="O154_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01772.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALES
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            5910..6809
                     /gene="rmlD"
     CDS             5910..6809
                     /gene="rmlD"
                     /note="O154_06"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ01773.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHF
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAMRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            6867..7745
                     /gene="rmlA"
     CDS             6867..7745
                     /gene="rmlA"
                     /note="O154_07"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01774.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFINAEQVQVLAESL
                     KKNAYGQYLLKVVEGY"
     gene            7732..8310
                     /gene="rmlC"
     CDS             7732..8310
                     /gene="rmlC"
                     /note="O154_08"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ01775.1"
                     /translation="MKVINKMKVIKTSIPDVLIFEPKVYGDERGFFMESFNQKVFDEA
                     VGRKIEFVQDNHSQSSKGVLRGLHYQLEPYAQGKLVRCVEGEVFDVAVDIRKSSPTFG
                     QWVGVYLNAKNKRQLWIPEGFAHGFIVTSEKATFLYKATNYYMPSHEGGIIWNDKSLN
                     IQWPLMREMIISAKDQQQISLTEYLEIESSKK"
     gene            8344..9636
                     /gene="wzx"
     CDS             8344..9636
                     /gene="wzx"
                     /note="O154_09"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01776.1"
                     /translation="MIRDFMLRFFSNKVKDHDFLKVFTNASYLTISQGVSYLIPLITL
                     SYVISLLGLEKFGRYSVILITATLLQVVTDYGFMFISPKEISIAGDNKQRVSEVYIST
                     TILKSLFLLLIFVFYTIVCYLIIDEYDLLINFLLGYLIVISQSLYPTWFFQGIQKMKI
                     IAGLSIIAKIINCLLVIFFLKISAEINILILSQAFPMFVVSFYANLIIIKKYIYLVKP
                     SWTSIRTLFLEGSGLFLANISSAILTNSTIPILSIYATPTQLGAYAAIERIIKSIYTI
                     FFPISQAIYPYNCKRFSYSLSDGITAVKKTGIPMVVFALITSVSIVITFYVMRNYIDI
                     YIYQYVLVVIFLSLWLIVGVINNVLGIQFLCAYNCKDFYSKAFFVSSSLTIPFMFWFS
                     SWIPAIGTSFAVLMGESLLGVILFIKIYNINKRVKLSD"
     CDS             9629..10735
                     /note="O154_10"
                     /gene="wffU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01777.1"
                     /translation="MINVVFCIRKDWKERPGGDVIQLVETKNAIESAYKCSINIISDP
                     DEILNIHPDIVHIFNMQTFEESKLFLTKAKQIGAFCVLSTVYWDMHDAFFVNAMQKMH
                     IYPSGKYFELLKRVFHLTCKVSVSIINKPYSLTNKYRKDMANFLGEFDAWLPNSEEEY
                     EIIQREFRLKKEKYIALNAVSNNVFCFNYNENRNGILCVGRFEPIKNQLSVCKALLNT
                     ELNLILIGAAHAHNKDYLKKIQKVNAVNNNIHIITKNIEQYELNSYYNKCRVHVLASF
                     RESPGLSSLEALATGANIVVSSSQFCPIDTYFKEIIGKHVFLCNPYSISSIRKAIEDA
                     YNAPLMRKSINRFSWDLTAKQTYLAYQEIINNRD"
     CDS             10743..11888
                     /note="O154_11"
                     /gene="mnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="BAQ01778.1"
                     /translation="MTTKIMLVFGTRPEAIKMAPLVNLLKRDSRYECKVCVTGQHREM
                     LHSVLDLFDITPDYNLNIMRPEQSLSTITVDIINGMSEIFKNFKPELIAIHGDTSTSF
                     AASLSAYYHQIKIAHIEAGLRTGNLYSPWPEEGNRKLTGVLADFHFCPTELTKQNLIR
                     EGVELNKLFITGNTVIDSLIYIINKIKKDGELLNYLHEKFSFIDQYRKMILITGHRRE
                     NFGTGFKNICQAIKNLAISNPDIIFVYPVHLNPNVQEPVNYYLKDVDNVKLIPPQEYL
                     PFVYLMERSYIILTDSGGIQEEAPSLGKPVLVMRETTERLEALNAGTVKLVGTNYDTI
                     IKEFEYLMNDKSHYESMSKSTNPYGNGNSSIKIKEIIDNYFFNSLQG"
     CDS             11854..12786
                     /note="O154_12"
                     /gene="wfgK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01779.1"
                     /translation="MIIIFLIPYKDENMNKPKVAVLLATYNGECWISAQIDSILAQRD
                     VDVTIYISDDCSTDKTKEICSNYSERFNNIKLLSNSERMGGASANFFRLLLSNEYDQH
                     DFVALSDQDDIWKENKLIHAIKQIGTDGFYSSNVTAFYSDGRQVLINKSQKMTSYDYF
                     FEGGGPGCTYVFSVENAFKLREFLVKHVNILKDITYHDWFIYAFARSNNYKWIIDPWP
                     SMYYRQHSNNQIGANSSFGGIIKRLKMIRSKWYRKQVTLIANTLEAADSSKIQKNLNR
                     NYYGNLLFSVNFYKLRRSYKDKIFILIFMLLYLF"
     gene            12805..13845
                     /gene="wzy"
     CDS             12805..13845
                     /gene="wzy"
                     /note="O154_13"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01780.1"
                     /translation="MEHMNRVYCLNTTFLNPLIPILLMVAVFILSSFLYKVDSSSLVI
                     IISTFLVISFIPLSFCFSSKYYVNTSNLSLKSFPLPYTTTIIIILSSSVEYFKLGIPL
                     FGHVIYAEFGYPFLHHVSVMSWLLLFTCNSFKNRIIFILVMVFAFVNPLLMINRDLML
                     LTCFVFIFILLGKRKVSLKPILILGSIFLFVFGLIGEYRSPGVIHTVDLPFSFNYEKM
                     PATIAWLFLYFTSSSFNMYYNIKTLGLNLYASNINVFPEPYYWSSFFDSFIFFPIVII
                     LYFILLLSIRHYLLTKKTSELTLALYLYCIYQVYTGLFAVKVFNTHTLFVMLFLGVIQ
                     FCNILLPKKRRS"
     CDS             13847..14746
                     /note="O154_14"
                     /gene="wekN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01781.1"
                     /translation="MHKQRVAILLSHYNGSDYLREQLNSILNQNADNFSIDIYVRDDG
                     SNNVNLVNLRQMTSCLPINVFEGSNIGVTKSFFTLLEQVDSYDYYAFCDQDDVWLDDK
                     ISSAVTMMKDSEGPVLYCSAYTLVDAKLNLINFENHVNDSFENALFKNFCTGCTCLIN
                     NKLRSIILKTDYTKNVPMHDWWMLMVAYLTGTVIFDQQSHILYRQHGGNVVGGRTSFI
                     KKTRRFMFNILSNDETRSKMYQQLLISKELYNSEKVALLELILNSKRNIFLRIKLVMG
                     LGVKYAKKYESILVKFLILIGRF"
     CDS             15181..15699
                     /note="O154_15; transposase of IS630 family"
                     /gene="IS630"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     gene            15979..17385
                     /gene="gnd"
     CDS             15979..17385
                     /gene="gnd"
                     /note="O154_16"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01782.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVVPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAVVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17634..18800
                     /gene="ugd"
     CDS             17634..18800
                     /gene="ugd"
                     /note="O154_17"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01783.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRGADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKENSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            18910..19926
                     /gene="wzz"
     CDS             18910..19926
                     /gene="wzz"
                     /note="O154_18"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01784.1"
                     /translation="MRTWKFPSVRVMMRVENNNVSGQNHDPEQIDLIDLLVQLWRGKM
                     TIIISVIVAIALAIGYLVVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVPD
                     LQEALIGRFSSAFSALAETLDNQKEPEKLTIEPSVKNQQLPLTVSYVGQTAEDAQMKL
                     AQYIQQVDDKVNQELEKDLKDNLALGRKNLQDSLRTQEVVAQAQKDLRIRQIQEALQY
                     ANQAQVTKPQIQQTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPDYYQTRQNLLD
                     IENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRN
                     YNSK"
     gene            complement(20023..20634)
                     /gene="hisI"
     CDS             complement(20023..20634)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01785.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6205 a   4027 c   4568 g   6162 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tatctgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac
      181 tggggcatcg gcataggcct tgcgggaagc acttacgata ataattaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacaccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca gctggataac
      541 acccatcttg cttataaatt gcgcggcatt caaatctctg ccgggaatgc cgtctccttt
      601 gtggcactga ctaacattga gatgaagcgt gcgtctctgg agttacacaa caaaccgcaa
      661 catcttttta tgcgtaatat caaagtgatg caggaatcct ctgttggacc agcattgagc
      721 atgaactttg acatgcgcaa agacgttcgc ggtgtcttta tggcgaaaaa agaaacactg
      781 ctgtcacttg caaatgttca tgcgatgaat gaaagagggc aaagctccgt cgacatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc
      961 tcacactaac tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc
     1021 tttatcttgt gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt
     1081 gctcatagga gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt
     1141 taatatcaag aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg
     1201 cgatgttcgt gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtact
     1261 actggcagcg gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt
     1321 tcagggtacc cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt
     1381 taccagttcc gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca
     1441 cgaccctttc aaccactacg gcaaaagtaa gtggcaggca gaggaagtgc tgcgtgaatg
     1501 gtataacaaa gcaccaacag aacgttcatt aaccatcatc cgtcctaccg ttatcttcgg
     1561 tgaacgcaac cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat
     1621 gatggtgggc gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt
     1681 tatcaagtac aaactgaaga atgttgccgc aggttatgag gtttataatt acgttgataa
     1741 gccagatctg aacatgaacc agttggttgc tgaagttgaa caaagtctga acaaaaagat
     1801 cccttctatg cacttgcctt acccactagg aatgttgggt ggatattgct ttgatatcct
     1861 gagcaaaatt acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa aattctgcgc
     1921 aacaacacag tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct
     1981 gtcgcaaggt ctggatcgaa ctctgcagta tgaattcgtt catgccaaaa aagacgacat
     2041 aacgtttgtt tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga
     2101 ttattcggaa agaggtactg tgatcttacc cagcaatagt ggacacgcgg ctaagtgagt
     2161 aaactctcag tcagaggtga ctcacatgac aaaaacagta tcaaccagta aaaaaccccg
     2221 taaacagcat tcgcctgaat ttcgcagtga agccctgaag cttgctgaac gcatcggtgt
     2281 tactgccgca gcccgtgaac tcagcctgta tgaatcacaa ctctacaact ggcgcagtaa
     2341 acagcaaaat cagcagacgt cttctgaacg tgaactggag atgtctaccg agattgcacg
     2401 tctcaaacgc cagctggcag aacgggatga agagctggct atcctccaaa aggccgcgac
     2461 atacttcgcg aagcgcctga aatgaagtat gtctttattg aaaaacatca ggctgagttc
     2521 agcatcaaag caatgtgccg cgagctccgg gtggcccgca gcggctggta tacgtggtgt
     2581 cagcggcgga caaggataag cacgcgtcag cagttccgcc aacactgcga cagcgttgtc
     2641 ctcgcggctt ttacccggtc aaaacagcgt tacggtgccc cacgcctgac ggatgaactg
     2701 cgtgctcagg gttacccctt taacgtaaaa accgtggcgg caagcctgcg ccgtcaggga
     2761 ctgagggcaa aggcctcccg gaagttcagc ccggtcagct accgcgcaca cggcctgcct
     2821 gtgtcagaaa atctgttgga gcaggatttt tacgccagtg gcccgaacca gaagtgggca
     2881 ggagacatca cgtacttacg tacagatgaa ggctggctgt atctggcagt ggtcattgac
     2941 ctgtggtcac gtgccgttat tggctggtca atgtcgccac gcatgacggc gcaactggcc
     3001 tgcgatgccc tgcagatggc gctgtggcgg cgtaagaggc cccggaacgt tatcgttcac
     3061 acggaccgtg gaggccagta ctgttcagca gattatcagg cgcaactgaa gcggcataat
     3121 ctgcgtggaa gtatgagcgc aaaaggttgc tgctacgata atgcctgcgt ggaaagcttc
     3181 tttcattcgc tgaaagtgga atgtatccat ggagaacact ttatcagccg ggaaataatg
     3241 cgggcaacgg tgtttaatta tatcgaatgt gattacaatc ggtggcggcg gcacagttgg
     3301 tgtggcggcc tcagtccgga acaatttgaa aacaagaacc tcgcttaggc ctgtgtccat
     3361 attacgtggg taggatcact gattaaatta tcagtatgcg gaattccgag taatccgagt
     3421 aacccagatg ctgatggtgt gtagtgttat cctaaggttt aagtaaagtt atgcggaact
     3481 aagctttggt atatagccat aacgtttggc taacctatgg caataattta cgcactatca
     3541 gccacttaga ggtaggaaaa gaatgacgaa tttaaaagca gttataccgg tagcaggtct
     3601 tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga
     3661 caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct
     3721 cctggtaact cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct catatgaatt
     3781 agaatctctc cttgagcagc gcgtgaagcg tcaactgctt gcggaagtgc agtccatctg
     3841 tccaccgggc gtgaccatta tgaacgtgcg tcagggcgaa cctttaggtt taggccactc
     3901 cattttgtgt gcacgacccg ccattggtga caacccattt gtcgtggtgc tgccagacgt
     3961 tgtgatcgac gatgccagcg ccgacccgct acgctacaac cttgctgcca tgattgcgcg
     4021 cttcaatgaa acgggccgta gccaagtgct ggcaaaacgt atgccgggtg acctctctga
     4081 atactccgtc atccagacca aagaaccgct ggaccgtgaa ggcaaagtca gccgcattgt
     4141 tgaattcatc gaaaagccgg atcagccgca gacgctggac tcagacgtca tggccgttgg
     4201 tcgctatgtg ctttctgccg atatttggcc ggaacttgaa cgtactcagc ctggtgcgtg
     4261 gggacgtatt cagctgacag atgcaattgc tgagctggcg aaaaaacagt ccgttgatgc
     4321 aatgctgatg accggcgaca gttacgactg cggtaaaaaa atgggctata tgcaggcgtt
     4381 tgtgaagtat ggactacgca acctcaaaga aggggcgaag ttccgtaaag gtattgagaa
     4441 gctgttaagc gaataatgaa aatctgaccg aatgtaacgg ttgataggaa aattataacg
     4501 gcggtgaaga ttcgtgacga aagtaatttg gagcgaatct tcgggacgtt atttgtaaat
     4561 tttcataata acaaagagtt atcaatagta ttttagtcaa agttttcagg attttccttg
     4621 tttccagagc ggattggtaa gacaattagc ttttgaatct ttcgggtttg gcgcgggtgg
     4681 acaacgctcg tcacatcgta gacatgcatg cagtgctctg gtagctgtaa agccaggggc
     4741 ggtagcatgc attaatacct ctatcaatca aactgagagc cgcttatttc acagcatgct
     4801 ctgaagtaat atggaataaa ttaagtgaaa atacttgtta ctggtggcgc aggatttatt
     4861 ggttctgctg tagttcgtca cattataaat aatacgcagg atagtgttgt taatgtcgat
     4921 aaattaacgt acgccggaaa cctggaatca cttgctgatg tgtctgattc tgaacgctat
     4981 atttttgaac atgcggatat ttgcgatgct gctgcaatgg cacggatttt tgctcagcat
     5041 cagccggatg cagtgatgca cttggctgct gaaagccatg ttgaccgttc aattacaggt
     5101 cctgcggcat ttattgaaac caatattgtt ggtacttatg tccttttgga agccgctcgc
     5161 aattactggt ctgctcttga tagcgacaag aaaaatagct tccgttttca tcatatttct
     5221 actgacgaag tctatggtga tttgcctcat ccagatgaag taaataataa cgaagaatta
     5281 cccttattta ctgagacgac agcttacgca ccaagcagcc cttattccgc atcaaaagca
     5341 tccagcgatc atttagtccg cgcgtggaaa cgtacctatg gtttaccgac cattgtgact
     5401 aactgttcga ataactacgg tccttatcac tttccggaaa aattgattcc actagtaatt
     5461 cttaatgctc tggaaagtaa ggcattacct atttatggca aaggggatca aattcgtgac
     5521 tggctgtatg ttgaagatca tgcgcgtgcg ttatataccg tcgtaaccga aggtaaagcg
     5581 ggtgaaactt ataacattgg tggacacaac gaaaagaaaa acatcgatgt agtgctcact
     5641 atttgtgatt tgttggatga gattgtaccg aaagagaaat cttatcgtga gcaaattact
     5701 tatgttgccg atcgcccggg acacgatcgc cgttatgcga ttgatgcaga gaagattagc
     5761 cgcgaattgg gctggaaacc gcaggaaacg tttgagagcg ggattcggaa gacagtggaa
     5821 tggtacctgt ccaatacaaa atgggttgat aatgtgaaaa gtggtgccta tcaatcgtgg
     5881 attgaacaga actatgaggg ccgccagtaa tgaatatcct ccttttcggc aaaacagggc
     5941 aggtaggttg ggaactacag cgtgctctgg cacctctggg taatttgatt gctcttgatg
     6001 ttcattccac tgattactgt ggtgatttta gtaatcctga aggtgtagct gaaaccgtca
     6061 aaaaaattcg ccctgatgtt attgttaatg ctgctgctca taccgcagta gataaggctg
     6121 agtcagaacc cgaatttgca caattactca atgcgaccag cgttgaatca attgcaaaag
     6181 cggctaatga agttggggcc tgggtaattc atttctcaac tgattacgta ttccctggaa
     6241 atggcgacac gccatggctg gagactgatg caactgcacc gcttaatgtt tacggtgaaa
     6301 ccaagttagc tggagaaaaa gcattacaag agcagtgtgc gaagcacctt attttccgta
     6361 ccagctgggt atatgcaggt aaaggaaaca attttgccaa gacaatgtta cgtctggcaa
     6421 aagagcgtga agaattagcc gttattaatg atcagtttgg tgcgccaact ggcgcagagt
     6481 tgctggctga ttgtacggca catgccatgc gtgtggcact gaataaaccg gaagtcgcag
     6541 gtttgtacca tctggtagcc agtggtacca caacctggca cgattatgct gcgctggttt
     6601 ttgaagaggc gcgcaaagca ggcatacccc ttgcactcaa caagctcaac gcagtagcaa
     6661 caacagccta tcctacacca gctcgtcgtc cacataactc tcgccttaat acagaaaaat
     6721 ttcagcagaa ttttgcgctt gttttgcctg actggcaggt tggcgtgaaa cgaatgctca
     6781 acgaattatt tacgactaca gtaatttaat agtttttgca tcttgttcgt gatgatggag
     6841 caagatgaat tgaaaggaat gatgaaatga aaacgcgtaa aggtattatt ttagcgggtg
     6901 gttctggtac tcgtctttat cctgtgacta tggctgtcag taaacagcta ttacctatat
     6961 atgataagcc gatgatctat tacccgcttt ctacactgat gttagcgggt attcgcgata
     7021 ttttgattat cagtacgcca caggatactc ctcgttttca acaactgctt ggtgacggta
     7081 gccagtgggg gctaaatctt cagtacaaag tgcaaccgag tccagatggt cttgcgcagg
     7141 catttatcat cggtgaagag tttatcggtg gtgatgattg tgctttggtt ctcggtgata
     7201 atatctttta cggtcacgat ctgccgaagt taatggatgt cgctgttaac aaagaaagtg
     7261 gcgcaacggt atttgcctat cacgttaatg atcctgaacg ctacggtgtc gttgagtttg
     7321 ataaaaacgg tacggcaata agcctggaag aaaaaccgtt acaaccaaaa agtaattatg
     7381 cggtaactgg gctttatttc tatgataatg acgttgtcga aatggcgaaa aaccttaagc
     7441 cttctgcccg tggcgaactg gaaattaccg atattaaccg tatatatatg gagcaggggc
     7501 gtttatctgt tgccatgatg ggacgtggtt atgcatggct ggacacgggg acacatcaaa
     7561 gtcttattga agcaagtaac ttcattgcaa caattgaaga gcgtcagggg ctgaaagttt
     7621 cctgtccgga agaaattgct taccgtaaag gctttattaa tgctgaacaa gtgcaagtgt
     7681 tagctgaatc gctaaagaaa aatgcttatg ggcagtattt attaaaggtg gttgaaggtt
     7741 attaataaaa tgaaagtcat aaaaactagt attccggatg ttttaatttt tgaaccaaaa
     7801 gtatatggag atgaacgtgg cttttttatg gaaagcttta atcaaaaagt tttcgatgag
     7861 gctgtagggc gtaaaattga atttgtgcag gataaccact ctcaatctag taaaggcgtg
     7921 ttgcgagggt tacattatca attagaacct tatgctcaag gaaagcttgt tcgctgtgtt
     7981 gaaggtgaag tatttgacgt cgcagttgac atacgtaaat catcacccac ttttggacag
     8041 tgggtaggtg tatatttaaa tgctaaaaac aagcgacaat tatggatacc ggaaggattt
     8101 gctcatggat ttattgtaac tagcgaaaaa gccacattcc tttataaagc aactaattac
     8161 tatatgccct ctcatgaagg tgggataata tggaacgata aatcattgaa tatacaatgg
     8221 cctttaatga gagaaatgat tatatcagca aaagatcaac agcaaatatc gctgactgaa
     8281 tatttagaaa ttgaatcatc aaaaaaatga tgagttggta taaacccaaa ccaaaactaa
     8341 ctaatgatta gagatttcat gttaagattc ttttctaaca aagtaaaaga tcatgatttt
     8401 ttgaaggtgt ttaccaatgc gtcatattta accatatcac aaggggtatc ttatttgata
     8461 cccttaatta ctttaagtta tgttatatct ctattaggat tagaaaaatt tggtcgttac
     8521 tctgtaattc taataactgc gacattactt caagtcgtga cagattatgg ttttatgttt
     8581 atctcaccaa aagaaatatc tattgcaggt gataataagc aaagagtatc agaagtatat
     8641 atatcaacca ctatattaaa gtcgttattt ctactgttga tttttgtttt ttatacaata
     8701 gtatgttatt taataataga tgaatatgat ttattaatta attttctact tggttatttg
     8761 attgtaatat cacaatcttt atatcctact tggtttttcc aagggataca gaaaatgaaa
     8821 ataatagcag gactttctat aattgcaaaa attataaact gcttattagt tatatttttt
     8881 ttgaaaatta gtgcagagat aaatattcta atattatcac aagcttttcc aatgtttgtt
     8941 gtgtcatttt atgcaaattt aattataata aaaaaatata tatatctcgt gaaaccttct
     9001 tggacttcga ttcgaacgtt atttcttgaa ggtagtggtt tatttttggc caatatatca
     9061 tcagcaattc ttacaaactc aacaattcct atactatcta tatatgcaac tccgacacag
     9121 ttaggtgcgt atgcagcaat tgaaaggata ataaaatcaa tttatacaat tttttttcct
     9181 atatcacaag cgatatatcc ttataattgt aaacgtttct catatagcct gagtgatgga
     9241 attactgctg taaaaaaaac cggcataccg atggtggtat ttgctttgat cactagtgtt
     9301 tctattgtga ttacttttta tgtcatgaga aattatattg atatatacat ttatcaatat
     9361 gtattagttg taattttcct aagtctatgg cttattgtcg gggtgattaa taatgtctta
     9421 gggatacaat ttctatgcgc atacaattgt aaggattttt atagtaaagc tttttttgta
     9481 tcatcttcat taacgatacc ttttatgttt tggtttagtt cttggatacc tgcaatcggt
     9541 acttcgtttg ccgttttgat gggtgaatca ttactagggg taatactttt tataaagatc
     9601 tacaatatta ataaaagggt gaaattaagt gattaatgta gttttctgta taaggaaaga
     9661 ctggaaggag cgtcctggag gagatgtgat tcaattggtt gagactaaga acgcaattga
     9721 gtcagcgtat aaatgctcta ttaacattat cagtgatccg gatgaaattt tgaatattca
     9781 ccctgatatt gtccacatat tcaatatgca aacttttgag gaaagtaagt tatttcttac
     9841 aaaagcaaag caaatcggcg cattttgtgt tctttctacc gtttattggg atatgcatga
     9901 tgcattcttt gttaatgcaa tgcaaaaaat gcatatttat cctagtggaa aatattttga
     9961 acttttaaaa agagtatttc atttaacgtg taaagtaagt gtttctatta ttaataagcc
    10021 atattcgctt acaaataagt atagaaaaga tatggctaac tttctagggg agtttgatgc
    10081 atggttaccg aattcagaag aggagtatga aataattcag agagagttca gattaaaaaa
    10141 ggaaaaatat atagccttaa atgccgtaag taataatgtt ttttgtttta attataatga
    10201 gaatagaaat ggcatattat gtgttggaag atttgaacca ataaaaaatc agttatctgt
    10261 atgcaaagca ttattaaata cagaactcaa tttgatatta attggagctg ctcacgcaca
    10321 taataaagat tacttaaaaa aaatacaaaa agtcaatgct gttaataata atattcacat
    10381 aatcactaaa aatatcgagc aatatgaatt aaatagttat tacaataaat gccgtgtcca
    10441 tgtattagct tcttttcgtg aatctcctgg tttaagttca ttagaagctt tagccaccgg
    10501 tgcaaatatt gttgtatctt caagccagtt ttgtccaatt gatacttact ttaaagagat
    10561 aattgggaaa catgtctttt tatgtaatcc atactccata agttctataa gaaaagccat
    10621 tgaggatgct tataatgcac cgctaatgcg taaatccatt aatagatttt catgggatct
    10681 aacagcaaag cagacctatt tggcttatca agagataata aataacagag attgatatta
    10741 aaatgactac aaaaataatg ttagtatttg gtacaagacc tgaagccatt aaaatggctc
    10801 ctttagtaaa tttattaaaa agagattctc gatatgaatg taaggtttgt gtaactggtc
    10861 agcatagaga aatgttacat agtgtattgg atttatttga tattacacca gattacaatt
    10921 taaatataat gcggccggaa caaagtttat cgacaattac agtcgatata attaatggta
    10981 tgtcagaaat atttaaaaat ttcaagccag agctaatagc aattcatggt gacacgtcaa
    11041 cttcattcgc tgcctcgtta tcagcatact atcatcaaat taaaattgca catattgaag
    11101 ctgggctcag aactggcaat ttatattctc cttggcctga agagggtaat agaaaattaa
    11161 caggagtgct tgcggacttc catttttgcc ctaccgaatt aactaaacag aatttaatta
    11221 gagaaggcgt tgagttaaac aaactgttta taacaggaaa tacggtaatt gattctttaa
    11281 tttatataat taataaaatt aaaaaagatg gtgaattgct aaattactta catgagaaat
    11341 tttcatttat tgatcaatat cgcaaaatga tactcattac cggtcatcgg cgtgaaaatt
    11401 ttggtactgg atttaaaaat atttgtcagg ctataaaaaa tctcgcaatt agtaatccag
    11461 atataatatt tgtttatccg gttcatttaa atcctaatgt tcaagaacca gtcaattatt
    11521 acttaaaaga tgttgataat gtaaaattaa ttccacctca agaatatctt ccatttgttt
    11581 atttaatgga acgttcatat attatcttaa ctgattcagg aggtattcaa gaagaagctc
    11641 cttccttggg caaacctgtt ctcgttatgc gtgaaactac tgaaaggcta gaagctttaa
    11701 atgcgggtac cgtgaagtta gttggtacaa attatgatac tatcattaag gaatttgagt
    11761 atctaatgaa cgataagtca cattatgaaa gtatgtcaaa atctactaat ccatacggaa
    11821 atggtaattc atcaattaaa attaaagaaa taattgataa ttattttttt aattccttac
    11881 aaggatgaga atatgaataa acctaaagtt gctgttttac tggctacata caatggagag
    11941 tgttggatca gtgcacaaat cgatagcatt cttgcacaaa gagatgtcga cgttacaatt
    12001 tatataagtg atgattgctc aactgataaa acaaaagaga tttgcagtaa ctattcagaa
    12061 agatttaata atattaagct actttcaaat tcagaacgga tggggggcgc aagtgctaat
    12121 tttttccgat tattgctttc aaatgagtat gatcaacatg attttgttgc tttatctgat
    12181 caagatgata tttggaaaga aaacaaactt attcatgcaa ttaaacaaat tggaactgac
    12241 ggcttttatt ccagtaatgt aactgcattt tatagcgatg gaagacaagt tttaataaat
    12301 aaatcccaga agatgacctc ctatgattat ttttttgaag gtggggggcc cggttgtact
    12361 tacgtctttt cagttgaaaa tgcattcaaa cttagggaat ttctagttaa acatgttaat
    12421 attcttaaag atataaccta tcatgattgg tttatatatg cttttgcaag atcaaataat
    12481 tataaatgga tcattgatcc ttggccctca atgtattacc gacagcacag caataatcaa
    12541 ataggtgcta actcttcttt tggtggaatc attaaaagat taaaaatgat tagatcaaaa
    12601 tggtatagaa aacaagtaac tttgatcgct aatactttag aagcagcgga ttcttctaaa
    12661 atacaaaaaa atctcaatag aaattactat ggaaatctat tgttttcagt taacttttat
    12721 aagttgcgac gctcatataa agataagatt tttattttaa tctttatgtt gttgtatcta
    12781 ttttgattat gataattata taagatggaa catatgaaca gagtttactg tttgaataca
    12841 actttcttaa acccattaat tcctatatta ttaatggtag ctgttttcat attatcatct
    12901 tttttgtata aggttgatag tagttcactt gttattatta tctcaacctt cttggttatt
    12961 tcctttatcc cattatcatt ctgtttttca agtaaatatt atgtcaatac atcaaaccta
    13021 agtctgaagt catttccgtt accgtatacg acaacaatca ttataattct atcaagcagt
    13081 gttgaatatt ttaagttagg aattcctctt tttggacatg ttatatatgc agagtttggg
    13141 tatccttttt tgcaccatgt ttcagttatg tcatggttat tattatttac atgtaattcc
    13201 tttaagaata gaataatttt tatacttgta atggtttttg catttgttaa tccattgttg
    13261 atgattaatc gtgatttaat gctattaact tgttttgttt ttatttttat ccttttagga
    13321 aaacgaaagg ttagtctaaa accgatactt attttaggga gtatatttct ttttgttttt
    13381 ggtttgatcg gtgaatacag aagtccaggt gtaatacata cagttgattt acctttttct
    13441 ttcaactatg aaaaaatgcc agcaacgata gcatggcttt ttctatattt tacttcttct
    13501 agttttaata tgtattataa tattaaaaca ttaggcttga atttatatgc aagtaatata
    13561 aatgttttcc cagaacccta ttactggtca tctttttttg atagctttat tttttttcct
    13621 attgtcatta ttttgtattt tattttactg ttatcaatta gacattatct tttaacaaaa
    13681 aagacttcag aattaacatt ggcattatac ctttattgta tctaccaagt gtatacggga
    13741 ttatttgctg taaaagtttt taatacacat acattatttg ttatgctttt tctaggagta
    13801 atccaattct gtaatattct tttacctaaa aagagacgtt cataacatgc ataaacaaag
    13861 agtagctatt ctcctatctc attataatgg aagtgattat ttaagagaac agttaaattc
    13921 tattctcaac cagaatgcag ataatttttc tattgatata tatgtcaggg atgatggttc
    13981 taataatgtt aatttggtaa accttcggca aatgacatcc tgtttaccta ttaatgtttt
    14041 tgagggaagc aatataggtg tgactaaaag tttttttact ctcttggagc aggttgattc
    14101 atatgattat tatgcctttt gcgatcagga tgatgtttgg cttgatgata aaatttcaag
    14161 cgcagtgacc atgatgaagg attccgaagg tccagtatta tactgttcag cttatacctt
    14221 ggttgatgca aaattaaatt tgataaattt tgaaaatcac gtcaatgatt cttttgaaaa
    14281 tgcattattt aaaaactttt gcacaggttg tacatgtttg ataaataata agttacgctc
    14341 gattattttg aaaactgatt atactaaaaa tgtaccaatg cacgattggt ggatgttgat
    14401 ggttgcatat ttaacaggaa cggttatttt tgatcaacaa tcccatatcc tttatcggca
    14461 acatggaggt aatgttgtcg gtggtcgaac tagttttata aaaaaaacaa gaagatttat
    14521 gtttaatatt ttatctaatg atgaaaccag atcaaaaatg tatcagcaac tattgataag
    14581 taaagaattg tataattctg agaaagtagc gttattggag cttatactga acagtaaaag
    14641 aaatattttt cttagaatta agttggttat gggcttaggg gttaaatatg ctaagaaata
    14701 cgaaagtatt ctagttaagt ttcttatttt aattggtagg ttctaatata ccttaaatag
    14761 ctgcgcggaa tagtagatca ctttaaggga acttagcccg gattgtttga tctgatcaat
    14821 caccaaatca aaacaaatca ccaaccggac tgagcgatgc cgatcatagc accaattccc
    14881 cgtgacgaac gacgcctgat gcagaaagca atccagaaaa cgcacgataa aaattatgcc
    14941 cgcagactga tggccatact gatgctgcat cgggcgacct cgttagcgat gttggcagaa
    15001 tgctctgccg tggcagttcc ttcgtcggac gctgaattaa ttggttcacg ctgtcgggtg
    15061 ttgcaagact gaaatcatta tctgccggac gaccccacgc tggccttttg agcatatctg
    15121 cactttatta cgtgagctgg taaacatgct cacggcgatt ttggctacca gcgctcaggc
    15181 ttgagtacag aacttctggc aataaaaatc aatgagataa ccggctgcca gttatatgcc
    15241 gggaccgttt gccgctggtt gccgtctgca gggcttgtgt ggcgaagggc cgcaccaacc
    15301 ctgcgtatcc gtgacccgca taaagatgaa aagatggcgg caatccacaa agcactggac
    15361 gaatgctgcg cagagcatcc ggtcttttat gaagatgaag tgtatatcca tcttaatccc
    15421 aaaatcggtg cagactggca gctacgtgga caacagaaac gcgtggtaac accggggcag
    15481 aaggaaaaat attatctggc cggcaacagc aaaagttcaa cgctgttcat cagcctgctg
    15541 aagcggctta aagcgacata ccgtcgggcg aaaaccatca cgctgatcgt ggacaactac
    15601 attatccata aaagccggga aacacagcgc tggctgaagg agaaccgaag ttcagagtta
    15661 tttatcagcc ggtttactcg ccattggtgc atcatgtaga acggctatgg caggcacttc
    15721 gcgacacaat aacgcgcaat catcagtgcc ggtcaatatg gcaactgctg aaaaaagttc
    15781 gccatgttat ggaaaccgtc agccatgccc taaggcaaac atgggctggc aaaagtgtag
    15841 cggtattagg cgcagctatt tataagatta taaaactaat ctatttaact taaaatttac
    15901 tacctataaa ctcatagttg aatcgaaatt taattcttaa actacacctc ttcatgatcc
    15961 ccctgactgg agtaaacaat gtcaaagcaa cagatcggcg tagtcggtat ggcagtgatg
    16021 gggcgcaacc ttgcgctcaa catcgaaagc cgtggttata ccgtctctat tttcaaccgt
    16081 tcccgtgaga agacggaaga agtgattgcc gaaaatccag gcaaaaaact ggttccttac
    16141 tatacggtga aagagtttgt tgaatctctg gaaacgcctc gtcgcatcct gttaatggtg
    16201 aaagcaggtg caggcacgga tgctgctatt gattccctca agccttacct cgataaaggt
    16261 gacatcatca ttgatggtgg caataccttc ttccaggaca ccattcgtcg taaccgtgaa
    16321 ctttctgccg aaggctttaa cttcattggt accggtgtct ccggtggtga agaaggcgcg
    16381 ctgaaaggtc cttctattat gcctggtggg cagaaagaag cctatgaact ggttgtacca
    16441 atcctgacca aaatcgccgc agtagctgaa gacggggagc catgcgttac ctatattggt
    16501 gccgatggtg caggtcacta tgtgaagatg gttcacaacg gtattgaata cggcgatatg
    16561 cagctgattg ctgaagccta ttctctgctt aaaggtggcc tgaatctttc caacgaagaa
    16621 ctggcgcaga cctttaccga gtggaataac ggtgaactga gcagctacct gatcgacatc
    16681 accaaagata tcttcaccaa aaaagatgaa gacggtaact acctggttga tgtgatcctg
    16741 gatgaagcag caaacaaagg tacgggcaaa tggaccagcc agagtgcgct ggatcttggc
    16801 gaaccgctgt cgctgattac cgagtctgtt tttgcacgtt atatctcttc tctgaaagat
    16861 cagcgtgttg ccgcgtctaa agttctctct ggtccgcaag cgcagccagc aggcgacaaa
    16921 gctgagttca tcgaaaaagt tcgtcgtgcg ctgtatctgg gcaaaatcgt ttcttacgcg
    16981 cagggtttct ctcagctgcg tgctgcgtct gaagagtaca actgggatct gaactacggc
    17041 gaaatcgcga agattttccg tgctggctgc atcatccgtg cgcagttcct gcagaaaatc
    17101 accgatgctt atgccgaaaa tccgcagatc gctaacctgc tgctggctcc gtacttcaag
    17161 caaattgccg atgactacca gcaggcgctg cgtgatgtcg ttgcttatgc agtacagaac
    17221 ggtatcccgg ttccgacctt cgcggcagcg gttgcctatt atgacagcta ccgcgccgta
    17281 gttctgcctg caaacctgat ccaggcacag cgtgactatt tcggtgcgca tacttataag
    17341 cgcattgata aagaaggtgt gttccatact gaatggctgg attaatctga tttaaccaac
    17401 aataaaattg aggcccggcg tatattgcac cgggcttttt tttgccaaat atcttataga
    17461 ctaaatttca ctgcttaata ttaacttaat aaatatcagc tattcttata aagaaaatct
    17521 gaattgtttt tcgttgcgtt gaccatctga caacgtagcg ttaaaacttt tagctcttat
    17581 caggatgcta aaaacatcat gattcacagt taagttaatt ctgagagcat gaaatgaaaa
    17641 tcaccatttc cggtactggc tatgtaggct tatcaaacgg gcttctaatc gcacaaaatc
    17701 atgaggttgt ggcattagat attttaccgt cacgcgttgc tatgctgaat gatcggatat
    17761 ctcctattgt cgataaggaa attcagcagt ttttgcaatc agataaaata cactttaatg
    17821 ccacattaga taaaaatgaa gcctaccggg gtgctgatta tgtcatcatc gccactccaa
    17881 ccgactatga tcctaaaact aattatttca atacatccag tgtagaatca gtaattaaag
    17941 acgtagttga gataaaccct tatgcggtta tggtcatcaa atcaacggtt cccgttggtt
    18001 ttaccgcagc gatgcataaa aaatatcgta ctgaaaatat tatattctcc ccagaatttc
    18061 tccgtgaggg taaagccctt tacgataacc ttcatccgtc acgtattgtc atcggtgagc
    18121 gttcagaacg cgcagaacgt tttgctgcgt tgttacagga aggcgcgatt aagcaaaata
    18181 tcccgaccct gtttaccgac tccactgaag cagaagcgat taaacttttc gctaatacct
    18241 atctggcgat gcgcgtggcg tactttaacg aactggatag ctatgcagaa agtttgggtc
    18301 tgaatacgcg tcagattatc gaaggtgttt gtctcgaccc gcgtatcggc aaccattaca
    18361 acaatccgtc gtttggttat ggtggttatt gtttgccgaa agataccaaa cagttactgg
    18421 cgaattatca gtctgtgccg aataacctga tttcggcaat tgtcgatgct aaccgcacgc
    18481 gtaaagattt tattgccgat gccattttgt cacgcaagcc gcaagtggtg ggtatttatc
    18541 gtctgattat gaagagcggt tcagacaact tccgcgcgtc ttccattcag gggattatga
    18601 aacgtatcaa ggcgaaaggc gttgaagtca tcatttacga accggtgatg aaagaaaact
    18661 cattcttcaa ctctcgcctg gaacgagatc tcgccacttt caaacaacaa gctgacgtca
    18721 ttatctccaa tcgtatggca gaagagctta aggatgtggc agataaggta tacacccgcg
    18781 atctctttgg cagcgactaa catcttgtta tcagggctat ttacgccctg attgtctttt
    18841 gttacttcct caacaattct ttatttttat cacttatcct atagcattca cgaggattat
    18901 cgctaaacta tgcggacttg gaaatttccg tcagttaggg taatgatgag agtagaaaat
    18961 aataatgttt ctgggcaaaa ccatgacccg gaacagattg atttgattga tttactagtg
    19021 cagttgtggc gtggcaagat gacaatcatc atttccgtca ttgtggctat tgccctggct
    19081 attggatatt tggtagtagc gaaggagaaa tggacgtcaa cagcaattat cactcagccc
    19141 gacgtggggc aaattgctgg ctataacaat gccatgaatg ttatctatgg tcaggctgca
    19201 cctaaagtgc cggatttaca ggaggcgtta attggtcgct tcagttctgc cttctctgca
    19261 ttagcagaaa cgctggataa tcagaaagaa ccagaaaaac ttaccatcga accttctgta
    19321 aagaaccagc aattaccatt gactgtttct tatgttgggc aaactgcaga ggacgcacaa
    19381 atgaaattgg cccaatacat tcagcaagtt gatgataaag taaatcaaga gctagaaaag
    19441 gatctcaagg acaaccttgc tttgggacgg aaaaacttgc aggactcttt aagaactcag
    19501 gaagtggttg cgcaggcgca gaaagatctg cgtatccgtc agattcagga agcgttgcag
    19561 tatgcgaatc aggcgcaggt gacaaaaccg cagattcaac agactggcga agatatcaca
    19621 caagatacgt tgttcctttt ggggagcgaa gcgctggagt cgatgattaa gcatgaggca
    19681 actcgtccgt tggtgttttc gccagactac tatcagactc gtcaaaacct gctggatatc
    19741 gaaaacttaa aggttgacga tcttgatatt catgcttacc gttatgtgat gaaaccgacg
    19801 ttacctattc gtcgcgatag cccgaaaaag gcaattacct tgattctggc ggtgctgctg
    19861 ggcggcatgg ttggcgcggg gattgtgttg gggcgtaatg ctctgcgtaa ttacaactcg
    19921 aagtaatctt ttcggtttta aagaaaatgg gcagggtggt gacaccttgc ccgttttttt
    19981 tgccggatgc gacgacaata tcgcatccgc ttaccccgca actcactgat gccgtttacg
    20041 caggttctca atcaccgtcg ttaagtccag atcctgatcc tgcaatagca ccagcaggtg
    20101 atacatcaaa tcagatgcct cgttggtcag ctcaaagcgg tcatgtactg tcgctgccag
    20161 tgcggtttcc acgccttctt cgcccacttt ctgcgcaatg cgcttggtgc cgctggcata
    20221 cagtttggcg gtataggagg tttccgggtc ggcagattta cgttcggcga gcagttgttc
    20281 cagttgatac aggaacagcc actggtgagc ggtgtcgccg aagcagctgc tggtgccttt
    20341 gtggcaggtc gggccgatgg gattcgccag caacagtaac gtgtcgttgt cgcagtccgg
    20401 cgtaatattt actacgttga ggaaattgcc cgacgtttcg cctttggtcc acagtcgctg
    20461 tttagtgcgc gagaagaagg tgactttacc gctttcgatg gttttgtcca gggcttgcgg
    20521 gttcatatag cccagcatta acacttcacc ggataccgcg tgttgcacaa tcaccggcat
    20581 aagtccgtcg gttttttccc agtccagttc gcgacgttgt tgttctgtta acatatcctg
    20641 atctccacgc cctgtgttgc caggtacgct tttaattcac caatattgat tatttgtttg
    20701 tggaatacgg aagctgccag cgcgccgtca acgtcggcat cgcggaaggc ttcgaggaag
    20761 tgttccatgg tgcccgcgcc accggaggca atcagcggga cgtggcaaac ttcacgcact
    20821 tttttcagct gctcgaggtc gtaaccgtta cgcacaccgt cctgattcat catgttgagg
    20881 acgatttctc cggcaccgcg tttttgcact tcctctaccc agtcgagtgt ttcccactga
    20941 gtgacgcggg tgcggctttc at
//
