LOCUS       AY657020               12755 bp    DNA     linear   BCT 05-JUL-2005
DEFINITION  Escherichia coli O155 O-antigen gene cluster, partial sequence.
ACCESSION   AY657020
VERSION     AY657020.1  GI:56123313
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Guo,H., Kong,Q., Cheng,J., Wang,L. and Feng,L.
  TITLE     Characterization of the Escherichia coli O59 and O155 O-antigen
            gene clusters: the atypical wzx genes are evolutionary related
  JOURNAL   FEMS Microbiol. Lett. 248 (2), 153-161 (2005)
   PUBMED   15990253
REFERENCE   2  (bases 1 to 12755)
  AUTHORS   Guo,H., Kong,Q., Cheng,J., Wang,L. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2004) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 HongDa Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serovar="O155"
                     /db_xref="taxon:562"
     gene            1213..2229
                     /gene="wclE"
     CDS             1213..2229
                     /gene="wclE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclE"
                     /protein_id="AAV74549.1"
                     /db_xref="GI:56123314"
                     /translation="MGFILLSFLVATAYYATFKRGNDMGLNIELISVVITFFSNDESS
                     GTYLNNSVLSALNQTYRNIEVLVIDDCSPIKATSCLNGINDSRLSIIRLPQNGGMAAA
                     INHGIGLSSGSFIAFLDFDDLWYDFKLKEQVELIKDYIDKKLAEPEKICCYSKVLIKH
                     DNYEYTKPYRSIEEGEHVADYLFANGQFMQTSGMLISKSLLIKVKGMDSYKRHTDYQL
                     VLKLYQENAVFIYYDRVSYMYKITDKKGDYKFSQDWLCANKWMMTKRSIRYFKINTIL
                     RSMIDNHAFGTAFIYSVSNRFIWSFGSYLIRFSLKKLLPTRLLSTLIKHKNKAAFSVK
                     GREY"
     gene            2232..3185
                     /gene="wzy"
     CDS             2232..3185
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAV74550.1"
                     /db_xref="GI:56123315"
                     /translation="MGGFITRLVLLLPLFLLFYFPADHNKDYFNYQTDYDYAIGQFDY
                     LYELSVRFFRDILNYSFSSFWVIVIIVEIIFLTIYYNKVKYILIAYPGIISMSQFFYG
                     TQIRYALAMLIGLVVLKNSRRFIKVFGVLIASLFHYGAVISLLPLFICRYINERYFIF
                     LSKRNIFLIISVLTLLFFFFYSFESFVSMTRFSYYLGAEKFSDSKSTSSIIYAVVSLL
                     FILFLYSKNTAFRTNISKQSIVILILVLIFSPIAVLSGRTMLVYFIMEPLLIASAFTS
                     IKKNSPDYFISLSLSLSLFLFYIARCIYYIVTADFYFYDFL"
     gene            3195..4244
                     /gene="wclF"
     CDS             3195..4244
                     /gene="wclF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclF"
                     /protein_id="AAV74551.1"
                     /db_xref="GI:56123316"
                     /translation="MRIAHIYKKHYDSGDGITNVVENLYKLQKNNNADEYIDLPFMFD
                     NCSKGKAQYRYSGFIGMLYHLFKCKPNVIYLHGFYYLHYIILGVCFYFIKSKVVLIPH
                     CSLLNRAITYKKIRKLIYYKIFSLIYYDSIILIQYLNHEEQIGSKKFLFSPPESIIPN
                     GVNYTEKQCRSFDFLSLFYLGRCDINHKGIDILFEYLTKINQKIKLYGADPNEKIKLK
                     KLIQLKKLEQKVEIYDPVFNEDKERVFLNNNIFILLSRYEGLPISVLEALSYGCICLV
                     SSGTNMADEIVAANCGFKIDSVEDLISVWNKLRVMSIYELKEMSENSKALIKHKYAWQ
                     KIIISSEIELRKYVT"
     gene            4358..5173
                     /gene="wclG"
     CDS             4358..5173
                     /gene="wbwC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwC"
                     /protein_id="AAV74552.1"
                     /db_xref="GI:56123317"
                     /translation="MKFTVLLSLYNRESKHNLFDCLESIKSNTIKPEQIVIVYDGPIS
                     EELDSIVTGFIKYLPIEKIKISENIGLGNALNYGLNFCRHEIVFRMDTDDKCKSTRFE
                     RQLEIIKIKNDIVLMGSAIEEFNESMTVSHGVRFSAEYHDDIINYAKRRNPFNHMSVV
                     FKKSIIQGLGGYQHHYFMEDYNLWLRVIAAGYKTYNIPEVLVYVRGGDKMLGRRKGIP
                     YVISEVKLAKLKYNLKIDSNAGVIISATMRILPRLLPVFLLKQIYKVLRKSKS"
     CDS             5285..6691
                     /note="6-phosphogluconate dehydrogenase"
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="AAV74553.1"
                     /db_xref="GI:56123318"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPEKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAESYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPIPTFSAAVTYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            6827..8113
                     /gene="wzx"
     CDS             6827..8113
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAV74554.1"
                     /db_xref="GI:56123319"
                     /translation="MSNYIKNAGWIFCEKFVRIAVGFILFALISRVLNPGEFGLLSYY
                     QTIATMFLAITNLGFDNVLINEFNQNKKHNEIFSTAFWSRIIVSVFVIMLFAVGLYFD
                     DVVLKNKLVLLLCISSLVFQSQNTYIPFFQSQLQASVVTKISFCALIISSVFKVYLLL
                     INGDIMWFAFSYSFDFAVSFLFFAFFSYKRNYISINPKHFRLSVLKRLLRSSWPIIAS
                     SIIIVLYTRLDQLMIMRMLGADSVAIFSVALKISEAYLFVPAALVTSYYPLISKSPTR
                     ENIRFYFDVVFSSSVIMAVVVAIASYFVIPYMFGANYITSYNILIILLFGSIFSILGS
                     ACTNLMIVYDLSYLRLVRAVYGLLINFSLNILLIPKYGIIGAAYASFVSQIFASWLSN
                     SLNKKTIECFRIQSMTVVSLGLIGFRQLFIMLFRRG"
     gene            8123..8590
                     /gene="wclD"
     CDS             8123..8590
                     /gene="wclD"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclD"
                     /protein_id="AAV74555.1"
                     /db_xref="GI:56123320"
                     /translation="MFDSYERKKGIMQRIIFYFKTKLRGCREYYYSLKYYHQFKIPIS
                     NHLTIGIKTLGENKTTLPHPVGIVIGKNVNIGKKCIVYQNVTIGVSNNKFEDYPVIGN
                     NVTIYAGAIIIGKVIIGDNAIIGAGCIVTKDVPEGKIAIGSPMKIMDKKHNNY"
     gene            8689..9855
                     /gene="ugd"
     CDS             8689..9855
                     /gene="ugd"
                     /note="dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="AAV74556.1"
                     /db_xref="GI:56123321"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     IVDKEIEEYLATKDLNFRATTDKYEAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(9921..10925)
                     /gene="gla"
     CDS             complement(9921..10925)
                     /gene="gla"
                     /note="nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gla"
                     /protein_id="AAV74557.1"
                     /db_xref="GI:56123322"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMATLFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNVLEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRIYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            11277..12311
                     /gene="wzz"
     CDS             11277..12311
                     /gene="wzz"
                     /note="chain length determinant protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="AAV74558.1"
                     /db_xref="GI:56123323"
                     /translation="MTIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGP
                     AVPKVSDIQTSLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
BASE COUNT     3875 a   2193 c   2617 g   4070 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtgca atctatctgc ccgccgggcg tgactattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggtcactcc attttgtgtg cacgacccgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgcta
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccgctg
      421 gaccgtgaag gcaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgttggact cagacatcat ggccgtaggt cgttatgtgc tttctgctga tatttggccg
      541 gaacttgaac gcacgcagcc aggggcatgg ggacgtattc agctgacaga tgccatcgct
      601 gaactggcga aaaaacaatc tgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg ggctacgtaa cctgaaagaa
      721 ggggcgaagt tccgcaaagg tattgagaag ctgttaagcg aataatgaaa atctgaccga
      781 atgttacggt tgataagaaa attataacgg cagtgaagat tcgtggcgag agtaatttgt
      841 tgcgaatatt cctgccgtta ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggcaacgc tcgtcacatc gtagacatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgaaaattaa ggatgctaat
     1081 aaataattgg ttgtatgtat accacaactt attaacttat ttatgtgtaa gtctaaatag
     1141 tgtggtaatt cttatcagtt ggaagatttt tacacatata aactgaaaac atcacaatat
     1201 agtaaagtta aagtgggctt tattttattg tcatttttgg ttgcaacagc atattatgct
     1261 acctttaaaa gaggaaacga catggggtta aatatagaat taataagtgt tgtgattact
     1321 ttctttagta atgatgaatc ttcgggtaca tatttaaata atagtgtttt aagcgcatta
     1381 aaccaaacat atagaaacat tgaagtcttg gtcattgatg attgctcccc aataaaagca
     1441 actagctgtc ttaatggtat taatgatagt agattgtcaa ttataagact tccacaaaat
     1501 ggtggaatgg ccgcagctat taatcatgga attggtctgt catctggaag ttttattgca
     1561 tttcttgatt ttgatgacct ttggtacgat ttcaaactta aagagcaagt tgagttgatc
     1621 aaagattata ttgataaaaa gctggctgaa cctgaaaaaa tatgttgcta ttctaaggtt
     1681 ttaataaaac atgataatta cgaatatact aaaccttaca gaagtataga ggagggtgag
     1741 cacgtagcag attatttgtt tgctaatgga caatttatgc aaacaagtgg gatgcttata
     1801 tcaaaatcat tacttataaa agtcaaagga atggattctt ataaaagaca tacggattat
     1861 cagttagtat taaaactata tcaagaaaat gctgtgttta tttattatga tcgagtttca
     1921 tatatgtata aaataactga caaaaaagga gattacaaat tttcgcaaga ttggctttgt
     1981 gcaaataagt ggatgatgac aaaacgttct ataagatatt tcaaaattaa tacaatatta
     2041 agaagcatga ttgataatca tgcttttggt actgcgttta tatattcagt atctaacaga
     2101 tttatatggt cttttggctc gtatttaata cgattttctt taaaaaaatt attacctaca
     2161 cgcttattat caacgctgat taaacataaa aataaagcag cgttttctgt aaaaggaaga
     2221 gagtattgaa aatgggaggc tttattacaa gattagtttt attgctgcca ttatttttat
     2281 tattttattt tcctgcggac cataacaaag attattttaa ttatcaaaca gattatgact
     2341 atgccatagg gcaatttgat tacctatatg agctatccgt gagatttttt agagatatat
     2401 tgaactatag ttttagttca ttttgggtga ttgtgattat agtagaaatt attttcttga
     2461 cgatatatta taacaaggtg aaatatatcc ttattgcata cccgggtata atttcgatga
     2521 gccagttttt ttatggcact caaatacgtt atgctttggc aatgttaatt ggattagtag
     2581 tattaaaaaa tagtagacgc tttattaaag tttttggagt gttaattgct tcattattcc
     2641 actatggcgc cgtgatatca ttattaccat tatttatttg taggtatata aatgaaagat
     2701 atttcatttt tttgtctaaa cgaaatatat tcttgattat atctgtatta acattactgt
     2761 tttttttctt ctattctttc gagtcatttg tttcaatgac tcgtttttct tattatttag
     2821 gtgctgaaaa attttccgat tcaaaatcga cttcatctat aatatatgcg gttgtgagtc
     2881 tattgtttat tctgttttta tattcaaaaa atacagcgtt cagaaccaat ataagtaaac
     2941 aatctatagt aatactgatt ttagttttga ttttttcacc tatagcagtg ctatcaggtc
     3001 gcacgatgct cgtttatttt ataatggaac ctttgttaat agcatctgca ttcacctcta
     3061 taaagaaaaa tagtcctgat tattttatat ctttatcgtt atctttgtct ttatttttat
     3121 tctatattgc gagatgtatt tattatatcg taacggcaga tttttatttt tatgatttct
     3181 tataaggaga acaaatgagg atagctcata tatataaaaa acattatgat agcggagatg
     3241 gtatcactaa tgttgttgag aatttatata aacttcaaaa aaacaataat gcagatgaat
     3301 atatagattt accattcatg tttgataatt gctcaaaagg taaggctcaa tatagatatt
     3361 cagggtttat tgggatgcta tatcatttgt tcaaatgcaa gccaaatgta atatacttgc
     3421 atggtttcta ctatttgcat tatattattc ttggtgtttg tttttatttt atcaaatcaa
     3481 aagttgtttt aataccacat tgctctcttt taaatagagc aattacttat aaaaaaataa
     3541 gaaagttgat atattataaa atattctcat taatttatta tgattctata atattgattc
     3601 agtatttgaa ccacgaagag caaataggtt caaaaaaatt tttattctca cctcctgaat
     3661 ccattatacc aaatggtgtt aactatactg aaaaacaatg tcgatctttt gattttctat
     3721 ctctattcta tttagggcga tgtgatataa atcataaagg tattgacata ctatttgagt
     3781 atcttactaa gattaatcaa aaaataaaat tatatggtgc agatccaaat gaaaaaataa
     3841 aactaaagaa attaattcaa ttaaagaaat tagagcaaaa agttgaaata tatgatcctg
     3901 tctttaatga ggataaggag agggtattct tgaataataa tatatttata ttactttcga
     3961 gatatgaagg attacctatt agtgtgcttg aagcactttc ttatggatgt atttgtctag
     4021 tttcatctgg aacaaatatg gctgatgaaa ttgtagctgc gaactgcgga tttaaaattg
     4081 attctgtaga ggatcttatt tcagtatgga ataagctacg ggtaatgagt atatatgaat
     4141 taaaagaaat gtctgaaaat tcaaaagcat taattaaaca taaatacgct tggcaaaaaa
     4201 taatcatatc ttctgagatt gaattaagaa aatatgtaac ataagtcttt gtgcgtagat
     4261 tttgaatgtt ctatttattc tggttagaat tttatagtca attcgatgac atatattcac
     4321 acaatatttc atacagaaga ttaattagga acttaatgtg aaatttactg ttttactttc
     4381 cttatataat agagagtcta aacacaactt gttcgattgt cttgaaagta ttaagtcaaa
     4441 cactataaag ccagaacaga tagtcatagt ttatgatgga ccgatcagtg aagaactgga
     4501 tagtattgtt actggtttta tcaaatactt accgattgag aaaataaaaa tatcagaaaa
     4561 tattggacta ggcaacgcac ttaattatgg cttgaatttt tgtagacatg aaattgtttt
     4621 caggatggat acagacgata aatgtaaaag cacaagattt gaacggcaac ttgaaattat
     4681 aaaaataaaa aatgatatcg tgttgatggg gagtgctata gaagaattta acgaatcgat
     4741 gacagtctct catggtgtaa gattttcagc cgaatatcat gatgatataa taaattatgc
     4801 aaaaagaaga aacccattta atcatatgtc agtggttttt aaaaaatcaa taatacaggg
     4861 gctaggaggg tatcagcatc attattttat ggaagattat aacttgtggc ttcgggttat
     4921 cgctgctggg tataaaactt ataatattcc agaagtgctt gtctatgttc gaggtggaga
     4981 taaaatgctt gggcgtagaa aaggaattcc ctatgtgatt agtgaggtaa aattggctaa
     5041 attaaaatat aacttaaaaa ttgacagtaa tgcaggtgtt attatttctg caaccatgag
     5101 gattttacct cgtttattgc ccgtcttttt gttaaaacaa atatataaag ttttaaggaa
     5161 aagcaagtct tgaattctct gtgaaattta tgaatacaat ccacattgat tacatatact
     5221 gaacattatc cgtttgttat aataccgcac aattctaagt caattcccct gacaggagta
     5281 aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc
     5341 gcttaatatc gaaagccgtg gttataccgt ctctattttc aaccgttctc gtgaaaagac
     5401 ggaagaagtg attgccgaaa atccagagaa gaaactggtt ccttactata cggtaaaaga
     5461 gtttgttgaa tctctggaaa cacctcgtcg catcctgtta atggtgaaag caggtgcagg
     5521 cacggatgct gctattgatt ccctcaagcc atatctcgat aaaggcgaca tcatcattga
     5581 tggtggtaat accttcttcc aggacaccat tcgtcgtaac cgtgagcttt ctgcggaagg
     5641 ctttaacttc attggtaccg gtgtttccgg tggtgaagag ggtgcgctga aaggtccttc
     5701 cattatgccg ggtggtcaga aagaagccta tgagctggtt gctccgatcc tgaccaaaat
     5761 cgctgctgtg gctgaagacg gtgagccatg cgttacctat atcggtgctg atggcgcagg
     5821 tcactatgtg aagatggttc acaacggtat tgaatacggc gatatgcagt tgattgctga
     5881 atcctattct ctgttgaaag gtggcctaaa cctctccaac gaagaactgg cgcagacctt
     5941 taccgagtgg aataacggtg aactgagcag ctacctgatc gacatcacca aagacatctt
     6001 cactaaaaaa gatgaagacg gtaattacct ggttgatgta attctggatg aagcggctaa
     6061 caaaggtacc ggtaaatgga ccagccagag tgcgctggat ctcggcgaac cgctgtcgct
     6121 gatcaccgag tctgtgtttg cacgttatat ctcttctttg aaagagcagc gtgttgccgc
     6181 gtctaaagtt ctctctggtc cacaagcaca gccagcaggc gataaggctg agtttatcga
     6241 gaaagttcgt cgtgcgctgt atctgggcaa aatcgtttct tacgctcagg gcttctctca
     6301 gctgcgtgct gcgtctgaag agtacaactg ggatctgaat tacggcgaaa tcgcgaagat
     6361 tttccgtgct ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcatatgc
     6421 cgaaaatccg cagatcgcta acctgctgct ggctccgtac tttaaacaaa ttgccgatga
     6481 ttaccagcag gcgctgcgcg atgtcgttgc ttatgcagta caaaacggta tcccgattcc
     6541 gaccttttcc gctgcggtta cctattacga cagctaccgt gcggctgttc tgcctgcaaa
     6601 cctgatccag gcacagcgtg actatttcgg tgcgcatact tataagcgca ttgataaaga
     6661 aggtgtgttc cataccgaat ggctggatta atttttttct agtttttaaa gagcctgctt
     6721 acgcggctct ttaatatata taaaaattat aattcagtcg attcaatcta gataaaacta
     6781 ttatttttat tatttcttag ttctaagagt ctacgtgaga tattagatgt ctaactatat
     6841 caaaaatgct ggttggattt tttgcgaaaa atttgtacga atagccgtag gctttatatt
     6901 atttgcatta atttcaagag ttttgaatcc tggtgaattt ggcttgctat cttattatca
     6961 aacaatagca accatgtttt tagcaataac caatttaggt ttcgataatg ttctgattaa
     7021 cgagtttaat cagaataaaa agcataatga aatattttca acggcatttt ggtcaagaat
     7081 tatagtttca gtatttgtaa taatgttatt tgctgtggga ttgtattttg atgatgtcgt
     7141 gctgaagaat aaattggttt tgctgttgtg tataagtagc ttggtttttc aaagtcaaaa
     7201 tacatacata ccattttttc aatctcagtt acaagcctca gttgtcacga aaattagttt
     7261 ttgcgcactt attatatcct cggtatttaa agtctatttg ttgttaatca atggggatat
     7321 aatgtggttt gctttttcat atagttttga ttttgctgtg tcttttttat ttttcgcttt
     7381 tttttcatat aaaagaaatt atatatcaat taatccaaaa catttccggt taagtgtgct
     7441 aaaacgttta ttacgctcgt cttggccaat tattgcatca tcaataatta ttgttctcta
     7501 tactcgatta gatcaattaa tgattatgcg aatgttaggg gctgattcag ttgcaatatt
     7561 tagtgttgct ttgaaaatat cagaggcata tttatttgtc cctgcagcat tagttacatc
     7621 atattatcca ctgatttcaa agtcacctac cagagaaaat attagatttt attttgatgt
     7681 tgttttttca agctctgtaa taatggctgt agtggtagct atagcgtctt attttgtaat
     7741 accatatatg tttggggcta attatatcac ctcatataat atattaatta ttttgctatt
     7801 tggttcaata ttttctatac tcggctcagc gtgtacaaat ttaatgatag tgtatgactt
     7861 atcttattta cggttagttc gagccgttta tgggctatta ataaatttct ccctgaatat
     7921 tttacttatt cctaaatatg gtattattgg tgctgcttat gcgtcttttg taagtcagat
     7981 ctttgcaagt tggttaagca atagccttaa taaaaaaacg attgaatgct tcagaataca
     8041 gtcaatgaca gttgtatctt tgggactaat aggatttaga caattattta tcatgctatt
     8101 tagaaggggg taggcatatt atatgtttga cagttatgaa agaaagaaag gtataatgca
     8161 aaggataatc ttttatttta aaacgaaatt gagaggatgt cgtgaatact attacagtct
     8221 taaatattat catcagttta aaattccaat ctcgaatcat cttaccatcg gcattaagac
     8281 attaggggaa aacaaaacta ctttacctca cccggtaggt atagttatag gtaagaacgt
     8341 taacatcggt aaaaaatgta ttgtttatca aaatgtcact ataggggtga gtaataataa
     8401 atttgaggat tatcctgtta ttggaaataa tgttactatt tatgccggag caatcataat
     8461 aggtaaagta attatagggg ataatgcaat aattggtgca ggttgcatcg ttactaaaga
     8521 tgtacctgaa ggaaagattg caattggttc accaatgaaa attatggata aaaaacataa
     8581 taactactaa ttatctcagt gaattcttaa taattttaaa atgtgttgac tgtaatgttc
     8641 gttttttgct attaatgtga aaaaacttca ttaaaaaaga atgcaaatat gaaaataaca
     8701 atttcaggaa caggttatgt tggtctttca aatggtattc tgattgcgca aaatcacgaa
     8761 gtggttgcac tggatatcgt tcaggccaaa gtggacatgc ttaacaagag gcagtcaccg
     8821 attgttgata aggagattga agagtatctg gcgactaaag atctcaattt ccgcgctacg
     8881 acagataagt atgaggcgta taaaaatgcc gattacgtta ttattgccac acctaccgat
     8941 tatgatccga aaacaaatta ttttaatacc tcaagtgtgg aagcggtcat tcgtgatgtg
     9001 acagaaatta atcccaacgc ggtaatgatt ataaaatcaa ctatccctgt tggttttaca
     9061 gagtccatta aagaacggtt tggtattgaa aatgtgatct tttcgccgga gtttttacgc
     9121 gaagggaaag cactttatga taacttgcat ccatcacgca ttgtgattgg tgagcaatcc
     9181 gatcgcgctg aacgttttgc agcattatta caagaaggag ctattaagca agatattcca
     9241 acgttgttta ctgactcaac ggaagctgag gcgattaaac tttttgccaa cacctatctg
     9301 gcgatgcgtg tagcgtattt caatgaactt gatagttatg ctgaaagctt ggggcttaat
     9361 tcacgccaga ttattgaggg cgtatgcctt gatccgcgta taggtaatca ctacaacaat
     9421 ccgtcattcg gttatggtgg ttattgtctg ccaaaagata ctaagcagtt actggcaaac
     9481 tatcagtcag taccgaataa cttgatctca gcaattgtcg atgccaatcg cacgcgaaaa
     9541 gattttattg ccgattctat ccttgcacgt aaaccgaaag ttgttggcgt ctatcgtctg
     9601 attatgaaga gtggttcgga caatttccgt gcttcatcga ttcagggtat tatgaagcga
     9661 atcaaggcga aaggtgtgcc agtaatcgtc tatgagccag cgatgaaaga ggacgatttt
     9721 ttccgttcgc gcgtggtacg tgatctggat gcgttcaaac aagaagctga tgttattatt
     9781 tctaaccgta tgtctgccga tctggctgat gtagcagata aggtttatac gcgcgacttg
     9841 tttggcaatg attaattatt ttgtttcata ctaagaaaag gccctaataa attagggcct
     9901 tttcttatgg ttttgtaaaa tcatacttta tagaaattac gataccattc tacaaagttc
     9961 tttaccccct ctttaactga cgtttcaggt ttgaatccta ttacgtcata tagtgcttta
    10021 gtatcagcac tggtttccag tacatcaccg ggttggagag gcatcatatt tttgttggct
    10081 tcaataccca gagcctcttc taaagcattg atatagtcca tcaactccac aggcgaacta
    10141 ttaccaatgt tataaatacg atatggtgct gaacttgttg caggcgaccc agtttctaca
    10201 gtccactgtg gatctttttc tggaataaca tcctgtaacc gaataatggc ttcggcaata
    10261 tcatcaatat aggtaaagtc acgcttcatt ttgccgaagt tgtatacatc aatgctttta
    10321 ccttccagca tagctttagt gaatttaaat aatgccatat ccggacgtcc ccatggacca
    10381 taaaccgtaa agaaacgcag ccctgtggtc ggtaagccat acaaatgaga ataggtatgg
    10441 gccatgagtt cattcgcttt tttagttgct gcataaagcg aaacaggatg atctacagag
    10501 tcatctgtag agaaaggcat cttgcggttc ataccataaa cagaactgga ggaagcgtag
    10561 agtagatgct gaacattatt atggcggcat ccttccagta cgttcaggaa tccaatcagg
    10621 tttgcatctg catatgcatt gggattttca agggagtaac gtacaccggc ttgcgcagcg
    10681 aggtttatta cgcgatcgaa ccgctcgtct gcaaacagtg tcgccatttt ctcacgatcg
    10741 gccaggtcaa ttttataaaa actgaaattg tcgtgcttga gtaaatcaag tcgtgcttgt
    10801 ttgaggttga catcgtaata atcatttaag ttgtcaatgc ctacaacctg atgaccagct
    10861 gcaagaagcc gtttacttac atagaaaccg ataaagccag cagctcccgt aaccagaaat
    10921 ttcatttata atcctcgctc aggctagaat atagccaatt ttcatctggc ataactgaaa
    10981 gttaaatcat accgttagac aagaaaaaaa gataatcggt atcagttcta aacctggctg
    11041 ttttttctgg taacgtgctc attttacaat caaagctgtt ctaagctgac tatacaagcc
    11101 gacgtcatta tttccaaccg tatggcagaa gagcttaagg atgtggcaga taaggtctac
    11161 acccgcgatc tttttggcag cgactaacat cctgttatca gggcgatttt cgccctgatt
    11221 ctcttatgtt ccctttgtag taattcattc tttttatcat ttatcctata gcattcatga
    11281 cgattatcgc taaattatgg cgacttaaaa ttatttcgtc cgttagggta atgatgagag
    11341 tagaaaataa taatgtttct gggcaaaacc atgacccgga acagattgat ttgattgatt
    11401 tactggtgca gttgtggcgc ggcaagatga caattatcat ctctgtcatt gtcgctattg
    11461 ccctggctat agggtatctg gccgttgcca aagaaaagtg gacttccaca gctatcgtta
    11521 ctcaacctga tgtggggcaa atcgctggtt ataccaacgc gatgaatgtg atttacggtc
    11581 cggctgtacc gaaagtctcc gacattcaga cttcacttat cggacgttac agcactgcat
    11641 tctcagcatt agcggaaacg ttggataatc aggaagaacc tgaaaaactg accattgagc
    11701 ctactgttaa aaatcagtcc ttacctttag cggtgtctta tgttggtgaa acagctgaag
    11761 gtgcgcagaa acagctggcg caatatatcc agcaagttga cgaccaggta aacgaagaac
    11821 tggaaaaaga cctgaaagac aacatcgcgc tgcgtatgaa aaacttgcag gattcgctga
    11881 aaacccagga agtagtcgcg caggagcaga aagatctgcg tatccgtcag attcaggaag
    11941 cgttgcagta tgcgaatcag gcgcaggtga caaagccaca ggttcagcag actgaagatg
    12001 tgacacaaga tacgttgttc cttttgggga gcgaagcgct ggagtcgatg attaagcatg
    12061 aggcgacccg tccgttggtg ttctcatcaa actactatca gactcgtcaa aacctgctgg
    12121 atatcgacaa tcttgacgtt gataaacttg atattcatgc ttaccgctat gtaatgaaac
    12181 cgacgttacc tattcgtcgc gatagcccga aaaaggcaat taccttgatt ctggcggtgc
    12241 tgctgggcgg catggttggc gcggggattg tgctggggcg taacgctctg cgtaattaca
    12301 acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccgtt
    12361 ttttttgccg gatgcgacaa caatatcgca tccgcttacc ccgcaactca ctgatgccgt
    12421 ttacgcaggt tctcaattac cgtagttaaa tccagatcct gatcttgcaa cagcaccagc
    12481 aggtgataca tcaaatcaga cgcctcgttg gtcagctcaa agcggtcatg tacagtcgcg
    12541 gccagtgcgg tttccacgcc ttcttcgccc actttctgcg caatgcgttt ggtgccgctg
    12601 gcatacagtt tggcggtgta ggaggtttcc gggtcggcag atttgcgttc ggcgagcagt
    12661 tgttccagtt gatacaggaa cagccactgg tgagcggtgt cgccgaagca gctgctggtg
    12721 cctttgtggc aggttggtcc gataggattc gccag
//
