LOCUS       AF061251               14002 bp    DNA     linear   BCT 20-AUG-1998
DEFINITION  Eschericia coli serotype O157:H7 O antigen gene cluster.
ACCESSION   AF061251
VERSION     AF061251.1  GI:3435170
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Wang,L. and Reeves,P.R.
  TITLE     Organization of Escherichia coli O157 O antigen gene cluster and
            identification of its specific genes
  JOURNAL   Infect. Immun. 66 (8), 3545-3551 (1998)
   PUBMED   9673232
REFERENCE   2  (bases 1 to 14002)
  AUTHORS   Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-1998) Microbiology, The University of Sydney, NSW
            2006, Australia
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C664-1992"
                     /serotype="O157:H7"
                     /db_xref="taxon:562"
     gene            60..842
                     /gene="wbdN"
     CDS             60..842
                     /gene="wbdN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAC32339.1"
                     /db_xref="GI:3435171"
                     /translation="MNKETVSIIMPVYNGAKTIISSVESIIHQSYQDFVLYIIDDCST
                     DDTFSLINSRYKNNQKIRILRNKTNLGVAESRNYGIEMATGKYISFCDADDLWHEKKL
                     ERQIEVLNNECVDVVCSNYYVIDNNRNIVGEVNAPHVINYRKMLMKNYIGNLTGIYNA
                     NKLGKFYQKKIGHEDYLMWLEIINKTNGAICIQDNLAYYMRSNNSLSGNKIKAAKWTW
                     SIYREHLHLSFPKTLYYFLLYASNGVMKKITHSLLRRKETKK"
     gene            839..2023
                     /gene="wzy"
     CDS             839..2023
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAC32340.1"
                     /db_xref="GI:3435172"
                     /translation="MKSAAKLIFLFLFTLYSLQLYGVIIDDRITNFDTKVLTSIIIIF
                     QIFFVLLFYLTIINERKQQKKFIVNWELKLILVFLFVTIEIAAVVLFLKEGIPIFDDD
                     PGGAKLRIAEGNGLYIRYIKYFGNIVVFALIILYDEHKFKQRTIIFVYFTTIALFGYR
                     SELVLLILQYILITNILSKDNRNPKIKRIIGYFLLVGVVCSLFYLSLGQDGEQNDSYN
                     NMLRIINRLTIEQVEGVPYVVSESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGE
                     RLHKQVFGDMGANFLSVTTYGAELLVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSIN
                     CAFYSYIIMILLQYLVAGNASAFFFGPFLSVLIMCTPLILLHDTLKRLSRNENISYNC
                     DL"
     gene            1992..2738
                     /gene="wbdO"
     CDS             1992..2738
                     /gene="wbdO"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAC32341.1"
                     /db_xref="GI:3435173"
                     /translation="MKISVITVTYNNAEGLEKTLSSLSILKIKPFEIIIVDGGSTDGT
                     NRVISRFTSMNITHVYEKDEGIYDAMNKGRMLAKGDLIHYLNAGDSVIGDIYKNIKEP
                     CLIKVGLFENDKLLGFSSITHSNTGYCHQGVIFPKNHSEYDLRYKICADYKLIQEVFP
                     EGLRSLSLITSGYVKYDMGGVSSKKRILRDKELAKIMFEKNKKNLIKFIPISIIKILF
                     PERLRRVLRKMQYICLTLFFMKNSSPYDNE"
     gene            2725..4116
                     /gene="wzx"
     CDS             2725..4116
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAC32342.1"
                     /db_xref="GI:3435174"
                     /translation="MIMNKIKKILKFCTLKKYDTSSALGREQERYRIISLSVISSLIS
                     KILSLLSLILTVSLTLPYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFAC
                     GKNLKMSRQISGGLTLLAGLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKV
                     FVIIFGLGIYSNGVQKVYMGIQKAYISNIVNAIFILLSIITLVISSKLHAGLPVLIVS
                     TLGIQYISGIYLTINLIIKRLIKFTKVNIHAKREAPYLILNGFFFFILQLGTLATWSG
                     DNFIISITLGVTYVAVFSITQRLFQISTVPLTIYNIPLWAAYADAHARNDTQFIKKTL
                     RTSLKIVGISSFLLAFILVVFGSEVVNIWTEGKIQVPRTFIIAYALWSVIDAFSNTFA
                     SFLNGLNIVKQQMLAVVTLILIAIPAKYIIVSHFGLTVMLYCFIFIYIVNYFIWYKCS
                     FKKHIDRQLNIRG"
     gene            4113..5213
                     /gene="per"
     CDS             4113..5213
                     /gene="per"
                     /codon_start=1
                     /transl_table=11
                     /product="perosamine synthetase Per"
                     /protein_id="AAC32343.1"
                     /db_xref="GI:3435175"
                     /translation="MKMKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKF
                     AEQNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFV
                     DSDNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEA
                     FGSKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHR
                     QYWHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKD
                     VFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDL
                     GWRGINLPSFPSLSNEQVIYICESINEFYSDK"
     gene            5238..6452
                     /gene="wbdP"
     CDS             5238..6452
                     /gene="wbdP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAC32344.1"
                     /db_xref="GI:3435176"
                     /translation="MKIALNSDGFYEWGGGIDFIKYILSILETKPEICIDILLPRNDI
                     HSLIREKAFPFKSILKAILKRERPRWISLNRFNEQYYRDAFTQNNIETNLTFIKSKSS
                     AFYSYFDSSDCDVILPCMRVPSGNLNKKAWIGYIYDFQHCYYPSFFSKREIDQRNVFF
                     KLMLNCANNIIVNAHSVITDANKYVGNYSAKLHSLPFSPCPQLKWFADYSGNIAKYNI
                     DKDYFIICNQFWKHKDHATAFRAFKIYTEYNPDVYLVCTGATQDYRFPGYFNELMVLA
                     KKLGIESKIKILGHIPKLEQIELIKNCIAVIQPTLFEGGPGGGVTFDAIALGKKVILS
                     DIDVNKEVNCGDVYFFQAKNHYSLNDAMVKADESKIFYEPTTLIELGLKRRNACADFL
                     LDVVKQEIESRS"
     gene            6472..7590
                     /gene="gmd"
     CDS             6472..7590
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-D-mannose dehydratase Gmd"
                     /protein_id="AAC32345.1"
                     /db_xref="GI:3435177"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            7587..8558
                     /gene="fcl"
     CDS             7587..8558
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="fucose synthetase Fcl"
                     /protein_id="AAC32346.1"
                     /db_xref="GI:3435178"
                     /translation="MMMNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLL
                     DSSAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVN
                     KLLFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDY
                     RSVMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDD
                     MASASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDT
                     TKPDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            8561..9070
                     /gene="wbdQ"
     CDS             8561..9070
                     /gene="wbdQ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdQ"
                     /protein_id="AAC32347.1"
                     /db_xref="GI:3435179"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            9052..10500
                     /gene="manC"
     CDS             9052..10500
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-P guanosyltransferase ManC"
                     /protein_id="AAC32348.1"
                     /db_xref="GI:3435180"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            10504..11874
                     /gene="manB"
     CDS             10504..11874
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase ManB"
                     /protein_id="AAC32349.1"
                     /db_xref="GI:3435181"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
     gene            13137..13802
                     /gene="wbdR"
     CDS             13137..13802
                     /gene="wbdR"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdR"
                     /protein_id="AAC32350.1"
                     /db_xref="GI:3435182"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
BASE COUNT     4540 a   2259 c   2824 g   4379 t
ORIGIN
        1 gcataaattt taatgcttat caaaactatt agcattaaaa atatataaga aattctcaaa
       61 tgaacaaaga aaccgtttca ataattatgc ccgtttacaa tggggccaaa actataatct
      121 catcagtaga atcaattata catcaatctt atcaagattt tgttttgtat atcattgacg
      181 attgtagcac cgatgataca ttttcattaa tcaacagtcg atacaaaaac aatcagaaaa
      241 taagaatatt gcgtaacaag acaaatttag gtgttgcaga aagtcgaaat tatggaatag
      301 aaatggccac ggggaaatat atttcttttt gtgatgcgga tgatttgtgg cacgagaaaa
      361 aattagagcg tcaaatcgaa gtgttaaata atgaatgtgt agatgtggta tgttctaatt
      421 attatgttat agataacaat agaaatattg ttggcgaagt taatgctcct catgtgataa
      481 attatagaaa aatgctcatg aaaaactaca tagggaattt gacaggaatc tataatgcca
      541 acaaattggg taagttttat caaaaaaaga ttggtcacga ggattatttg atgtggctgg
      601 aaataattaa taaaacaaat ggtgctattt gtattcaaga taatctggcg tattacatgc
      661 gttcaaataa ttcactatcg ggtaataaaa ttaaagctgc aaaatggaca tggagtatat
      721 atagagaaca tttacatttg tcctttccaa aaacattata ttatttttta ttatatgctt
      781 caaatggagt catgaaaaaa ataacacatt cactattaag gagaaaggag actaaaaagt
      841 gaagtcagcg gctaagttga tttttttatt cctatttaca ctttatagtc tccagttgta
      901 tggggttatc atagatgatc gtataacaaa ttttgataca aaggtattaa ctagtattat
      961 aattatattt cagatttttt ttgttttatt attttatcta acgattataa atgaaagaaa
     1021 acagcagaaa aaatttatcg tgaactggga gctaaagtta atactcgttt tcctttttgt
     1081 gactatagaa attgctgctg tagttttatt tcttaaagaa ggtattccta tatttgatga
     1141 tgatccaggg ggggctaaac ttagaatagc tgaaggtaat ggactttaca ttagatatat
     1201 taagtatttt ggtaatatag ttgtgtttgc attaattatt ctttatgatg agcataaatt
     1261 caaacagagg accatcatat ttgtatattt tacaacgatt gctttatttg gttatcgttc
     1321 tgaattggtg ttgctcattc ttcaatatat attgattacc aatatcctgt caaaggataa
     1381 ccgtaatcct aaaataaaaa gaataatagg gtatttttta ttggtagggg ttgtatgctc
     1441 gttgttttat ctaagtttag gacaagacgg agaacaaaat gactcatata ataatatgtt
     1501 aaggataatt aataggttaa caatagagca agttgaaggt gttccatatg ttgtttctga
     1561 atctattaag aacgatttct ttccgacacc agagttagaa aaggaattaa aagcaataat
     1621 aaatagaata cagggaataa agcatcaaga cttattttat ggagaacggt tacataaaca
     1681 agtatttgga gacatgggag caaatttttt atcagttact acgtatggag cagaactgtt
     1741 agtttttttt ggttttctct gtgtattcat tatcccttta gggatatata taccttttta
     1801 tcttttaaag agaatgaaaa aaacccatag ctcgataaat tgcgcattct attcatatat
     1861 cattatgatt ttattgcaat acttagtggc tgggaatgca tcggccttct tttttggtcc
     1921 ttttctctcc gtattgataa tgtgtactcc tctgatctta ttgcatgata cgttaaagag
     1981 attatcacga aatgaaaata tcagttataa ctgtgactta taataatgct gaagggttag
     2041 aaaaaacttt aagtagttta tcaattttaa aaataaaacc ttttgagatt attatagttg
     2101 atggcggctc tacagatgga acgaatcgtg tcattagtag atttactagt atgaatatta
     2161 cacatgttta tgaaaaagat gaagggatat atgatgcgat gaataagggc cgaatgttgg
     2221 ccaaaggcga cttaatacat tatttaaacg ccggcgatag cgtaattgga gatatatata
     2281 aaaatatcaa agagccatgt ttgattaaag ttggcctttt cgaaaatgat aaacttctgg
     2341 gattttcttc tataacccat tcaaatacag ggtattgtca tcaaggggtg attttcccaa
     2401 agaatcattc agaatatgat ctaaggtata aaatatgtgc tgattataag cttattcaag
     2461 aggtgtttcc tgaagggtta agatctctat ctttgattac ttcgggttat gtaaaatatg
     2521 atatgggggg agtatcttca aaaaaaagaa ttttaagaga taaagagctt gccaaaatta
     2581 tgtttgaaaa aaataaaaaa aaccttatta agtttattcc aatttcaata atcaaaattt
     2641 tattccctga acgtttaaga agagtattgc ggaaaatgca atatatttgt ctaactttat
     2701 tcttcatgaa gaatagttca ccatatgata atgaataaaa tcaaaaaaat acttaaattt
     2761 tgcactttaa aaaaatatga tacatcaagt gctttaggta gagaacagga aaggtacagg
     2821 attatatcct tgtctgttat ttcaagtttg attagtaaaa tactctcact actttctctt
     2881 atattaactg taagtttaac tttaccttat ttaggacaag agagatttgg tgtatggatg
     2941 actattacca gtcttggtgc tgctctgaca tttttggact taggtatagg aaatgcatta
     3001 acaaacagga tcgcacattc atttgcgtgt ggcaaaaatt taaagatgag tcggcaaatt
     3061 agtggtgggc tcactttgct ggctggatta tcgtttgtca taactgcaat atgctatatt
     3121 acttctggca tgattgattg gcaactagta ataaaaggta taaacgagaa tgtgtatgca
     3181 gagttacaac actcaattaa agtctttgta atcatatttg gacttggaat ttattcaaat
     3241 ggtgtgcaaa aagtttatat gggaatacaa aaagcctata taagtaatat tgttaatgcc
     3301 atatttatat tgttatctat tattactcta gtaatatcgt cgaaactaca tgcgggacta
     3361 ccagttttaa ttgtcagcac tcttggtatt caatacatat cgggaatcta tttaacaatt
     3421 aatcttatta taaagcgatt aataaagttt acaaaagtta acatacatgc taaaagagaa
     3481 gctccatatt tgatattaaa cggttttttc ttttttattt tacagttagg cactctggca
     3541 acatggagtg gtgataactt tataatatct ataacattgg gtgttactta tgttgctgtt
     3601 tttagcatta cacagagatt atttcaaata tctacggtcc ctcttacgat ttataacatc
     3661 ccgttatggg ctgcttatgc agatgctcat gcacgcaatg atactcaatt tataaaaaag
     3721 acgctcagaa catcattgaa aatagtgggt atttcatcat tcttattggc cttcatatta
     3781 gtagtgttcg gtagtgaagt cgttaatatt tggacagaag gaaagattca ggtacctcga
     3841 acattcataa tagcttatgc tttatggtct gttattgatg ctttttcgaa tacatttgca
     3901 agctttttaa atggtttgaa catagttaaa caacaaatgc ttgctgttgt aacattgata
     3961 ttgatcgcaa ttccagcaaa atacatcata gttagccatt ttgggttaac tgttatgttg
     4021 tactgcttca tttttatata tattgtaaat tactttatat ggtataaatg tagttttaaa
     4081 aaacatatcg atagacagtt aaatataaga ggatgaaaat gaaatatata ccagtttacc
     4141 aaccgtcatt gacaggaaaa gaaaaagaat atgtaaatga atgtctggac tcaacgtgga
     4201 tttcatcaaa aggaaactat attcagaagt ttgaaaataa atttgcggaa caaaaccatg
     4261 tgcaatatgc aactactgta agtaatggaa cggttgctct tcatttagct ttgttagcgt
     4321 taggtatatc ggaaggagat gaagttattg ttccaacact gacatatata gcatcagtta
     4381 atgctataaa atacacagga gccaccccca ttttcgttga ttcagataat gaaacttggc
     4441 aaatgtctgt tagtgacata gaacaaaaaa tcactaataa aactaaagct attatgtgtg
     4501 tccatttata cggacatcca tgtgatatgg aacaaattgt agaactggcc aaaagtagaa
     4561 atttgtttgt aattgaagat tgcgctgaag cctttggttc taaatataaa ggtaaatatg
     4621 tgggaacatt tggagatatt tctactttta gcttttttgg aaataaaact attactacag
     4681 gtgaaggtgg aatggttgtc acgaatgaca aaacacttta tgaccgttgt ttacatttta
     4741 aaggccaagg attagctgta cataggcaat attggcatga cgttataggc tacaattata
     4801 ggatgacaaa tatctgcgct gctataggat tagcccagtt agaacaagct gatgatttta
     4861 tatcacgaaa acgtgaaatt gctgatattt ataaaaaaaa tatcaacagt cttgtacaag
     4921 tccacaagga aagtaaagat gtttttcaca cttattggat ggtctcaatt ctaactagga
     4981 ccgcagagga aagagaggaa ttaaggaatc accttgcaga taaactcatc gaaacaaggc
     5041 cagtttttta ccctgtccac acgatgccaa tgtactcgga aaaatatcaa aagcacccta
     5101 tagctgagga tcttggttgg cgtggaatta atttacctag tttccccagc ctatcgaatg
     5161 agcaagttat ttatatttgt gaatctatta acgaatttta tagtgataaa tagcctaaaa
     5221 tattgtaaag gtcattcatg aaaattgcgt tgaattcaga tggattttac gagtggggcg
     5281 gtggaattga ttttattaaa tatattctgt caatattaga aacgaaacca gaaatatgta
     5341 tcgatattct tttaccgaga aatgatatac attctcttat aagagaaaaa gcatttcctt
     5401 ttaaaagtat attaaaagca attttaaaga gggaaaggcc tcgatggatt tcattaaata
     5461 gatttaatga gcaatactat agagatgcct ttacacaaaa taatatagag acgaatctta
     5521 cctttattaa aagtaagagc tctgcctttt attcatattt tgatagtagc gattgtgatg
     5581 ttattcttcc ttgcatgcgt gttccttcgg gaaatttgaa taaaaaagca tggattggtt
     5641 atatttatga ctttcaacac tgttactatc cttcattttt tagtaagcga gaaatagatc
     5701 aaaggaatgt gttttttaaa ttgatgctca attgcgctaa caatattatt gttaatgcac
     5761 attcagttat taccgatgca aataaatatg ttgggaatta ttctgcaaaa ctacattctc
     5821 ttccatttag tccatgccct caattaaaat ggttcgctga ttactctggt aatattgcca
     5881 aatataatat tgacaaggat tattttataa tttgcaatca attttggaaa cataaagatc
     5941 atgcaactgc ttttagggca tttaaaattt atactgaata taatcctgat gtttatttag
     6001 tatgcacggg agctactcaa gattatcgat tccctggata ttttaatgaa ttgatggttt
     6061 tggcaaaaaa gctcggaatt gaatcgaaaa ttaagatatt agggcatata cctaaacttg
     6121 aacaaattga attaatcaaa aattgcattg ctgtaataca accaacctta tttgaaggcg
     6181 ggcctggagg gggggtaaca tttgacgcta ttgcattagg gaaaaaagtt atactatctg
     6241 acatagatgt caataaagaa gttaattgcg gtgatgtata tttctttcag gcaaaaaacc
     6301 attattcatt aaatgacgcg atggtaaaag ctgatgaatc taaaattttt tatgaaccta
     6361 caactctgat agaattgggt ctcaaaagac gcaatgcgtg tgcagatttt cttttagatg
     6421 ttgtgaaaca agaaattgaa tcccgatctt aatatattca agaggtatat aatgactaaa
     6481 gtcgctctta ttacaggtgt aactggacaa gatggatctt atctagctga gtttttgctt
     6541 gataaagggt atgaagttca tggtatcaaa cgccgagcct catcttttaa tacagaacgc
     6601 atagaccata tttatcaaga tccacatggt tctaacccaa attttcactt gcactatgga
     6661 gatctgactg attcatctaa cctcactaga attctaaagg aggtacagcc agatgaagta
     6721 tataatttag ctgctatgag tcacgtagca gtttcttttg agtctccaga atatacagcc
     6781 gatgtcgatg caattggtac attacgttta ctggaagcaa ttcgcttttt aggattggaa
     6841 aacaaaacgc gtttctatca agcttcaacc tcagaattat atggacttgt tcaggaaatc
     6901 cctcaaaaag aatccacccc tttttatcct cgttcccctt atgcagttgc aaaactttac
     6961 gcatattgga tcacggtaaa ttatcgagag tcatatggta tttatgcatg taatggtata
     7021 ttgttcaatc atgaatctcc acgccgtgga gaaacgtttg taacaaggaa aattactcga
     7081 ggacttgcaa atattgcaca aggcttggaa tcatgtttgt atttagggaa tatggattcg
     7141 ttacgagatt ggggacatgc aaaagattat gttagaatgc aatggttgat gttacaacag
     7201 gagcaacccg aagattttgt gattgcaaca ggagtccaat actcagtccg tcagtttgtc
     7261 gaaatggcag cagcacaact tggtattaag atgagctttg ttggtaaagg aatcgaagaa
     7321 aaaggcattg tagattcggt tgaaggacag gatgctccag gtgtgaaacc aggtgatgtc
     7381 attgttgctg ttgatcctcg ttatttccga ccagctgaag ttgatacttt gcttggagat
     7441 ccgagcaaag ctaatctcaa acttggttgg agaccagaaa ttactcttgc tgaaatgatt
     7501 tctgaaatgg ttgccaaaga tcttgaagcc gctaaaaaac attctctttt aaaatcgcat
     7561 ggtttttctg taagcttagc tctggaatga tgatgaataa gcaacgtatt tttattgctg
     7621 gtcaccaagg aatggttgga tcagctatta cccgacgcct caaacaacgt gatgatgttg
     7681 agttggtttt acgtactcgg gatgaattga acttgttgga tagtagcgct gttttggatt
     7741 ttttttcttc acagaaaatc gaccaggttt atttggcagc agcaaaagtc ggaggtattt
     7801 tagctaacag ttcttatcct gccgatttta tatatgagaa tataatgata gaggcgaatg
     7861 tcattcatgc tgcccacaaa aataatgtaa ataaactgct tttcctcggt tcgtcgtgta
     7921 tttatcctaa gttagcacac caaccgatta tggaagacga attattacaa gggaaacttg
     7981 agccaacaaa tgaaccttat gctatcgcaa aaattgcagg tattaaatta tgtgaatctt
     8041 ataaccgtca gtttgggcgt gattaccgtt cagtaatgcc aaccaatctt tatggtccaa
     8101 atgacaattt tcatccaagt aattctcatg tgattccggc gcttttgcgc cgctttcatg
     8161 atgctgtgga aaacaattct ccgaatgttg ttgtttgggg aagtggtact ccaaagcgtg
     8221 aattcttaca tgtagatgat atggcttctg caagcattta tgtcatggag atgccatacg
     8281 atatatggca aaaaaatact aaagtaatgt tgtctcatat caatattgga acaggtattg
     8341 actgcacgat ttgtgagctt gcggaaacaa tagcaaaagt tgtaggttat aaagggcata
     8401 ttacgttcga tacaacaaag cccgatggag cccctcgaaa actacttgat gtaacgcttc
     8461 ttcatcaact aggttggaat cataaaatta cccttcacaa gggtcttgaa aatacataca
     8521 actggtttct tgaaaaccaa cttcaatatc gggggtaata atgtttttac attcccaaga
     8581 ctttgccaca attgtaaggt ctactcctct tatttctata gatttgattg tggaaaacga
     8641 gtttggcgaa attttgctag gaaaacgaat caaccgcccg gcacagggct attggttcgt
     8701 tcctggtggt agggtgttga aagatgaaaa attgcagaca gcctttgaac gattgacaga
     8761 aattgaacta ggaattcgtt tgcctctctc tgtgggtaag ttttatggta tctggcagca
     8821 cttctacgaa gacaatagta tggggggaga cttttcaacg cattatatag ttatagcatt
     8881 ccttcttaaa ttacaaccaa acattttgaa attaccgaag tcacaacata atgcttattg
     8941 ctggctatcg cgagcaaagc tgataaatga tgacgatgtg cattataatt gtcgcgcata
     9001 ttttaacaat aaaacaaatg atgcgattgg cttagataat aaggatataa tatgtctgat
     9061 gcgccaataa ttgctgtagt tatggccggt ggtacaggca gtcgtctttg gccactttct
     9121 cgtgaactat atccaaagca gtttttacaa ctctctggtg ataacacctt gttacaaacg
     9181 actttgctac gactttcagg cctatcatgt caaaaaccat tagtgataac aaatgaacag
     9241 catcgctttg ttgtggctga acagttaagg gaaataaata aattaaatgg taatattatt
     9301 ctagaaccat gcgggcgaaa tactgcacca gcaatagcga tatctgcgtt tcatgcgtta
     9361 aaacgtaatc ctcaggaaga tccattgctt ctagttcttg cggcagacca cgttatagct
     9421 aaagaaagtg ttttctgtga tgctattaaa aatgcaactc ccatcgctaa tcaaggtaaa
     9481 attgtaacgt ttggaattat accagaatat gctgaaactg gttatgggta tattgagaga
     9541 ggtgaactat ctgtaccgct tcaagggcat gaaaatactg gtttttatta tgtaaataag
     9601 tttgtcgaaa agcctaatcg tgaaaccgca gaattgtata tgacttctgg taatcactat
     9661 tggaatagtg gaatattcat gtttaaggca tctgtttatc ttgaggaatt gagaaaattt
     9721 agacctgaca tttacaatgt ttgtgaacag gttgcctcat cctcatacat tgatctagat
     9781 tttattcgat tatcaaaaga acaatttcaa gattgtcctg ctgaatctat tgattttgct
     9841 gtaatggaaa aaacagaaaa atgtgttgta tgccctgttg atattggttg gagtgacgtt
     9901 ggatcttggc aatcgttatg ggacattagt ctaaaatcga aaacaggaga tgtatgtaaa
     9961 ggtgatatat taacctatga tactaagaat aattatatct actctgagtc agcgttggta
    10021 gccgccattg gaattgaaga tatggttatc gtgcaaacta aagatgccgt tcttgtgtct
    10081 aaaaagagtg atgtacagca tgtaaaaaaa atagtcgaaa tgcttaaatt gcagcaacgt
    10141 acagagtata ttagtcatcg tgaagttttc cgaccatggg gaaaatttga ttcgattgac
    10201 caaggtgagc gatacaaagt caagaaaatt attgtgaaac ctggtgaggg gctttcttta
    10261 aggatgcatc accatcgttc tgaacattgg atcgtgcttt ctggtacagc aaaagtaacc
    10321 cttggcgata aaactaaact agtcaccgca aatgaatcga tatacattcc ccttggcgca
    10381 gcgtatagtc ttgagaatcc gggcataatc cctcttaatc ttattgaagt cagttcaggg
    10441 gattatttgg gagaggatga tattataaga cagaaagaac gttacaaaca tgaagattaa
    10501 catatgaaat ctttaacctg ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa
    10561 ctgaatgaag atattgcctg gcgcattggg cgtgcctatg gcgaatttct caaaccgaaa
    10621 accattgttt taggcggtga tgtccgcctc accagcgaag cgttaaaact ggcgcttgcg
    10681 aaaggtttac aggatgcggg cgtcgatgtg ctggatatcg gtatgtccgg caccgaagag
    10741 atctatttcg ccacgttcca tctcggagtg gatggcggca tcgaagttac cgccagccat
    10801 aacccgatgg attacaacgg catgaagctg gtgcgcgaag gggctcgccc gatcagcggt
    10861 gataccggac tgcgcgatgt ccagcgtctg gcagaagcca atgacttccc tcctgtcgat
    10921 gaaaccaaac gtggtcgcta tcagcaaatc aatctgcgtg acgcttacgt tgatcacctg
    10981 ttcggttata tcaacgtcaa aaacctcacg ccgctcaagc tggtgatcaa ctccgggaac
    11041 ggcgcagcgg gtccggtggt ggacgccatt gaagcccgat ttaaagccct cggcgcaccg
    11101 gtggaattaa tcaaagtaca caacacgccg gacggcaatt tccccaacgg tattcctaac
    11161 ccgctgctgc cggaatgccg cgacgacacc cgtaatgcgg tcatcaaaca cggcgcggat
    11221 atgggcattg cctttgatgg cgattttgac cgctgtttcc tgtttgacga aaaagggcag
    11281 tttatcgagg gctactacat tgtcggcctg ctggcagaag cgttcctcga aaaaaatccc
    11341 ggcgcgaaga tcatccacga tccacgtctc tcctggaaca ccgttgatgt ggtgactgcc
    11401 gcaggcggca ccccggtaat gtcgaaaacc ggacacgcct ttattaaaga acgtatgcgc
    11461 aaggaagacg ccatctacgg tggcgaaatg agcgctcacc attacttccg tgatttcgct
    11521 tactgcgaca gcggcatgat cccgtggctg ctggtcgccg aactggtgtg cctgaaagga
    11581 aaaacgctgg gcgaaatggt gcgcgaccgg atggcggcgt ttccggcaag cggtgagatc
    11641 aacagcaaac tggcgcaacc cgttgaggca attaatcgcg tggaacagca ttttagccgc
    11701 gaggcgctgg cggtggatcg caccgatggc atcagcatga cctttgccga ctggcgcttt
    11761 aacctgcgct cctccaacac cgaaccggtg gtgcggttga atgtggaatc acgcggtgat
    11821 gtaaagctaa tggaaaagaa aactaaagct cttcttaaat tgctaagtga gtgattattt
    11881 acattaatca ttaagcgtat ttaagattat attaaagtaa tgttattgcg gtatatgatg
    11941 aatatgtggg cttttttatg tataacgact ataccgcaac tttatctagg aaaagattaa
    12001 tagaaataaa gttttgtact gaccaatttg catttcacgt cacgattgag acgttccttt
    12061 gcttaagaca ttttttcatc gcttatgtaa taacaaatgt gccttatata aaaaggagaa
    12121 caaaatggaa cttaaaataa ttgagacaat agatttttat tatccctgtt tacgatatta
    12181 tagccaaagt tgtatcctgc atcagtcctg caatatttca cgagtgcttt gttaactgaa
    12241 tacatgtctg ccattttcca gatgataacg acgtcatcgc aattgatggt aaaacacttc
    12301 ggcacactta tgacaagagt cgtcgcagag gagtggttca tgtcattagt gcgtttcagc
    12361 aatgcacagt ctggtcctcg gatagatcaa gacggatgag aaacctaatg cgttcacagt
    12421 tattcatgaa ctttctaaaa tgatgggtat taaaggaaaa ataatcataa ctgatgcgat
    12481 ggcttgccag aaagatattg cagagaagat ataaaaacag agatgtgatt atttattcgc
    12541 tgtaaaagga aataagagtc ggcttaatag agtctttgag gagatattta cgctgaaaga
    12601 attaaataat ccaaaacatg acagttacgc aattagtgaa aagaggcacg gcagagacga
    12661 tgtccgtctt catattgttt gagatgctcc tgatgagctt attgatttca cgtttgaatg
    12721 gaaagggctg cagaatttat gaatggcagt ccactttctc tcaataatag cagagcaaaa
    12781 gaaagaatcc gaaatgacga tcaaatatta tattagatct gctgctttaa ccgcagagaa
    12841 gttcgccaca gtaaatcgaa atcactggcg catggagaat aagttgcaca gtagcctgat
    12901 gtggtaatga atgaaatcga ctataatata agaaggcgag ttgcattcga atgattttct
    12961 agaatgcggc acatcgctat taatatctga caatgataat gtattcaagg caggattatc
    13021 atgtaagatg cgaaaagcag tcatggacag aaacttccta gcgtcaggca ttgcagcgtg
    13081 cgggctttca taatcttgca ttggttttga taagatattt ctttggagat gggaaaatga
    13141 atttgtatgg tatttttggt gctggaagtt atggtagaga aacaataccc attctaaatc
    13201 aacaaataaa gcaagaatgt ggttctgact atgctctggt ttttgtggat gatgttttgg
    13261 caggaaagaa agttaatggt tttgaagtgc tttcaaccaa ctgctttcta aaagcccctt
    13321 atttaaaaaa gtattttaat gttgctattg ctaatgataa gatacgacag agagtgtctg
    13381 agtcaatatt attacacggg gttgaaccaa taactataaa acatccaaat agcgttgttt
    13441 atgatcatac tatgataggt agtggcgcta ttatttctcc ctttgttaca atatctacta
    13501 atactcatat agggaggttt tttcatgcaa acatatactc atacgttgca catgattgtc
    13561 aaataggaga ctatgttaca tttgctcctg gggctaaatg taatggatat gttgttattg
    13621 aagacaatgc atatataggc tcgggtgcag taattaagca gggtgttcct aatcgcccac
    13681 ttattattgg cgcgggagcc attataggta tgggggctgt tgtcactaaa agtgttcctg
    13741 ccggtataac tgtgtgcgga aatccagcaa gagaaatgaa aagatcgcca acatctattt
    13801 aatgggaatg cgaaaacacg ttccaaatgg gactaatgtt taaaatatat ataatttcgc
    13861 taatttacta aattatggct tctttttaag ctatccttta cttagttatt actgatacag
    13921 catgaaattt ataatactct gatacatttt tatacgttat tcaagccgca tatctagcgg
    13981 taacccctga caggagtaaa ca
//
