LOCUS       GU068044               14597 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain E1020-72 serogroup O158 O antigen gene
            cluster, complete sequence.
ACCESSION   GU068044
VERSION     GU068044.1  GI:307340804
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 14597)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1020-72"
                     /db_xref="taxon:562"
                     /note="serogroup: O158"
     gene            143..1252
                     /gene="gne"
     CDS             143..1252
                     /gene="gne"
                     /note="UDP-N-acetylglucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADN43865.1"
                     /db_xref="GI:307340805"
                     /translation="MVVFGTRPEAIKMAPLVHEIKKNPELELKVCVTAQHREMLDQVL
                     DLFNIKPDYDLNLMKTGQTLNDITSSILIHIKSILKNYNPDIVLVHGDTATTFAVSLA
                     AYYEQIVIGHIEAGLRTGNIYSPWPEEANRKLTSVLAEYHFAPTETAKINLLREGCPE
                     YKITVTGNTVIDALHMVKDKIDKNLSLRAKLDNQFPFLKNDKKIILVTGHRRESFGRG
                     FENICEALALIAKNHDDVQIVYPVHMNPNVQEPVNRILSGIDNIHLIEPQQYLQFIYL
                     MDRSYLIITDSGGIQEEAPSLGKPVLVMRETTERPEAIKAGTVKLVGANKENIVREVN
                     ELLDCKEAYNAMSFAHNPYGNGTSSKIIISKLISS"
     misc_feature    143..13063
                     /note="O antigen gene cluster"
     gene            1256..2341
                     /gene="rmlB"
     CDS             1256..2341
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6 dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADN43866.1"
                     /db_xref="GI:307340806"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMVRIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVPLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNENKNIDVVLAICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2341..3240
                     /gene="rmlD"
     CDS             2341..3240
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADN43867.1"
                     /db_xref="GI:307340807"
                     /translation="MNILLFGKTGRVGWELQRALAPLGNLIAFDIHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELEVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYLTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3298..4176
                     /gene="rmlA"
     CDS             3298..4176
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADN43868.1"
                     /db_xref="GI:307340808"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGINLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNNVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKEFIDANQIKVLAEPL
                     KKNVYGQYLLKMIKDY"
     gene            4181..4723
                     /gene="rmlC"
     CDS             4181..4723
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADN43869.1"
                     /db_xref="GI:307340809"
                     /translation="MNIIKTDIPDVLIFEPKVFYDERGFFFESFNQKVFEEAVAHKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFITLSDIAEFLYKTTSFYSPKHEVCIRFNDENIGIHWPNI
                     TDNTLISPKDKKGLNFNSLV"
     gene            5103..6356
                     /gene="wzx"
     CDS             5103..6356
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43870.1"
                     /db_xref="GI:307340810"
                     /translation="MSGVKKKLLKNFFSLSMLQGLNYLIPIITMPYLFRTLGPYYFGV
                     LAIATAAINYLVVITDYGFNFIATKDISVNRENKEKLIEIYSSVLTIKLLLCLLCFFI
                     LLLILFFSKQDCFFIQVYIFTYGIVLGQSLFSTWFFQGVEEMKYITLINVVFKLSATL
                     FIFIIVRSKDDVLFVPIISSLGYILSGLASFFIIQRKYLISFQPQTLMTIKVYFSKNW
                     NMFISNFFTLLYRNSNVLILGFFSSASIVASYAIAEKIIKAIQSIQMIFGTACYPYLA
                     KKFNNDHSSFYEISKKYWLIILLAYFLLTLIVYFLSPVAIYYSTGYSDFMAIQLMKIM
                     SFLILFGGLDYTLGVLGLIPMGYSKYFSNSVVITGIFSITLSALLSKYYGGYGASISI
                     VLCEVLLLVALSLKIKSLYLSRGRK"
     gene            6358..7509
                     /gene="wzy"
     CDS             6358..7509
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43871.1"
                     /db_xref="GI:307340811"
                     /translation="MPYIVISLSPFLAAPSFLSTLGTLSFFSLIIANLYFYFKMSTSA
                     VLPRTITPMICISFLFIICLIFSVLYHDVASKTINYFFFYVFMISCIIAISFISYTKF
                     IDLKKINKLTLYILIFLFIFAFIEYYYIITNNGYISWVPRFERPDYASNSFSGLYRIR
                     LFTYESSSAGALLYSLYLIFLYNIKITYQKNIFVLLISLALHLMIFSSTQFVFFMIII
                     IAQFFSSNLISRKAKIFLLILVLVGIVVASHEVGILINYISNKLDGFITGNASNASAS
                     ERNKLLLIGVNAFLENPIFGGGLASFYNYVDTGLVNFHLQTLQQLGIFGYLLFLTFLL
                     YPLLFFKQYTLEIKLLFILTWFTLFFSGSIWDSNLYLPYLFIAGKSNER"
     gene            7499..8569
                     /gene="wcmQ"
     CDS             7499..8569
                     /gene="wcmQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmQ"
                     /protein_id="ADN43872.1"
                     /db_xref="GI:307340812"
                     /translation="MKDKSFIMLCTPGYIKKTGYYFRAERDKDVLQSLNFKEKGTIVI
                     NSLKKIINTVLLIKKNKNTLFICENIAAALPIALFFLMYKKTSLKVILVYHGSLEDLK
                     AFKFYKLKRFIYEKIERIMIHRETTVLFVSNEMKEYYNIKYGDLESFHVSPNIPSDLF
                     FESINDAKNKEKNELRNLLSLPEEKKIVCYCGNSQSWQKVELLTKIINFNTDGDLFFL
                     ILTRDTDYFIKNISKSKVNNTIIKTVPNDEVPYYLVASDMLYILRDDNETNRFSCPTK
                     LVEYLWSQKPVLISENIGDFSQKIKCYNSGIVLNSNHYNDIDLITSLIKCFNGAAFPD
                     DELDNYTFSFQHNLFKAILSDI"
     gene            8584..9312
                     /gene="wcmR"
     CDS             8584..9312
                     /gene="wcmR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmR"
                     /protein_id="ADN43873.1"
                     /db_xref="GI:307340813"
                     /translation="MIKILFGIVLYSYTPKNSPTYSHLINNNINPDDIYLHDNREENI
                     KLSTVYNDFLNFANENSYDYLVILDDDTFLSDEYLKSLHSNLNCSVLIPNLTYEHKII
                     SPLKFKGILRFCMSGIRLHLSKLNSLKFSENYSLYGIDDYLVIFLIKNKIKVKISGAY
                     IEHELSTIRGIQGANSYSDFKCIELLRAHILNFKEAPLFLKIFYFKKISFFLFPLFKR
                     RNFLEPMKIFFSPAQRSMYIQEKD"
     gene            9315..10448
                     /gene="wcmS"
     CDS             9315..10448
                     /gene="wcmS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmS"
                     /protein_id="ADN43874.1"
                     /db_xref="GI:307340814"
                     /translation="MKIIFLSSYDPQDNKSWSGTPKRMLDALKSMDAEIIIIGPYDSK
                     IKKILKYMSFAVKKILKYNYAHSILASLEYKLFFQSVIKKHSDANFIFAPASSTEIAL
                     LNTSIPIIYSSDATFALIDSYYDNFSNLLKISRKESNFIEKKALDVSKFIIYPSVWAK
                     ESAINDYLIDKDKIKVIPWGANFDYIENTFNEKKITKSGKIKILFVGVDWYRKGGDIV
                     YKTCENIREKYNLDIELLICGCTPIGVDSNVNWIKCFGRLNKNLKEDLDMLIDLYSNA
                     HLLFVPSRNECYGMVFCEASAYSLPIISHNTGGIGSVVSHNVNGYLLDVDSPLQEYEA
                     KIMYLVNNPDIYRLFSINSRNIYEKKLNWRVWVKDLFSYINNY"
     gene            11945..13063
                     /gene="wcmT"
     CDS             11945..13063
                     /gene="wcmT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmT"
                     /protein_id="ADN43875.1"
                     /db_xref="GI:307340815"
                     /translation="MKTMASSANTRWYLYNFRVNTIRCLVKEGYRVLAIAPKDDYSEQ
                     LELLGSEFINIFIDQSGKNPIKDLITLFKFYSIFKRENVNCVLNFTPKNTIYSTLAAS
                     LANVKAINNIAGLGLLFIEECITSKIAGMLYKLSQRRASKIFFQNEEDRVFFLSQKIV
                     DISMTDRLPGSGVDLKRFYHSPSPDDGVVRFLLVARMLYDKGIGQYIEAARHLKSKYG
                     NNVEFRLLGFLDVNNPSAVTKEDMQSWVNGGIVIYLGTSDNVEREIAKADCMVLPSYY
                     REGIPKSLLESGAMGKPIVTTNNVGCRETVDDGINGFLCIPRSSESLIEKLDLIINMS
                     HEQRVEMGLASRKKIECEFNEEIVIKKYLDAIVQCLEN"
BASE COUNT     4733 a   2330 c   2811 g   4723 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgggtaatta gattgagaca atccgttgaa
       61 tgcgagatta ttaggttctg ttgtaatcct ttaatcgtat tcaactataa ttgaagagat
      121 aacaaaagtg attaaaaaga taatggttgt ttttggtaca agaccagaag caatcaaaat
      181 ggctcctctt gtgcatgaaa taaaaaagaa tcccgaatta gaactgaaag tatgtgtaac
      241 agcgcagcat cgtgaaatgt tggatcaagt tcttgatttg tttaatatta aaccagatta
      301 cgatctgaat cttatgaaga cgggtcaaac attaaatgat attacttcct ctattttaat
      361 tcacatcaaa tcgattctaa agaactataa tcctgatatt gtgcttgttc acggtgatac
      421 agcaactaca tttgcagtta gcttggctgc atattatgaa caaatagtca ttgggcatat
      481 agaggctggg ttacggacag gaaatatcta ttcaccttgg ccagaggaag cgaatcgtaa
      541 gttaacatca gtgttagcag aatatcattt cgctccaacg gaaacagcaa aaataaattt
      601 attaagagaa gggtgtcctg agtataaaat tactgttact gggaatactg ttattgatgc
      661 tctacatatg gtaaaagaca aaatagataa aaatttaagt cttagagcaa aattagataa
      721 tcaatttcct tttctaaaga acgataaaaa aataattctt gtcacaggtc atcgtcgtga
      781 aagttttggc agaggatttg aaaacatttg tgaagcatta gcattaattg caaaaaatca
      841 tgacgatgta cagatagttt atcctgtaca tatgaatcct aatgtacaag aacctgtgaa
      901 tagaattctt tcgggaattg acaatattca cttgattgaa ccgcagcagt acctgcagtt
      961 tatatattta atggaccgtt cttatctgat tataactgat tcgggaggta ttcaggaaga
     1021 agctccatct ttaggtaaac ctgttttggt tatgcgggaa accacagaac gtcccgaagc
     1081 tattaaggcg ggcacagtta aactggtcgg tgcgaataaa gaaaacatcg ttcgcgaagt
     1141 aaatgagctg ttggattgca aagaagctta taatgcaatg agttttgcac ataacccata
     1201 tggcaatggt acatcatcta aaataatcat ctctaaattg atatcttcat aaattatgaa
     1261 aatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa
     1321 taatacgcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa acttggaatc
     1381 acttgctgat gtttctgatt ctgaacgcta tgtttttgaa catgcggata tttgcgatgc
     1441 tgctgcaatg gtgcggattt ttgctctgca tcagccggat gcagtgatgc acttggctgc
     1501 tgaaagccat gtggatcgtt caattacagg ccctgcggca tttattgaaa ccaatattgt
     1561 tggtacttat gtccctttgg aagcggctcg caattactgg tctgctcttg atggcgacaa
     1621 gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtttatggcg atctgcctca
     1681 tcctgacgaa gtaaataata aagaagaatt acccttattt actgagacaa cagcttacgc
     1741 tccaagcagc ccttattctg catcaaaagc gtccagcgat catttagtcc gcgcgtggaa
     1801 acgtacctat ggtttaccga ccattgtgac taattgctct aacaattatg gtccttatca
     1861 tttcccggaa aaattgattc cattggttat tctgaatgct ctggaaggta aagcattacc
     1921 tatttacggc aaaggggatc aaattcgcga ctggctgtat gtagaggatc atgctcgagc
     1981 gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa
     2041 cgaaaataaa aacatcgatg tagtgctcgc tatttgtgat ttgttggatg agattgtccc
     2101 gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgcccgg gacacgatcg
     2161 ccgttatgcg attgatgctg agaagatcgg tcgcgaattg ggatggaaac cacaggaaac
     2221 gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa aatgggttga
     2281 taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta
     2341 atgaatatcc tccttttcgg caaaacaggg cgggtcggtt gggaactaca gcgtgctctg
     2401 gcacctctgg gtaatttgat tgcttttgat atccactcca ctgattattg tggtgatttt
     2461 agtaaccctg aaggtgtggc tgaaacagtc aaaagaattc gacctgatgt tattgttaat
     2521 gctgcggctc ataccgcagt agataaggct gagtcagaac ccgaatttgc acaattactc
     2581 aatgcgacta gcgttgaatc aattgcaaaa gcggctaatg aagttggggc ttgggtaatt
     2641 cattactcaa ctgactacgt attccctgga aatggcgaca cgccatggct ggagacggat
     2701 gcaaccgcac cgctaaatgt ttacggtgaa accaagttag ccggagaaaa agcgttacag
     2761 gaacattgcg cgaagcatct tattttccgt accagctggg tctatgcagg taaaggaaac
     2821 aattttgcca agacaatgtt acgtctggca aaagagcgtg aagaattaga ggttattaac
     2881 gatcagtttg gtgcgccaac aggtgctgaa ctgctggctg attgtacagc acatgccatt
     2941 cgtgtcgcac tgaataaacc ggatgtcgca ggcttgtacc atttggtagc cagtggtacc
     3001 acaacctggt acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc aggcattccc
     3061 cttgcactca acaagctcaa cgcagtacca acaactgcct atcttacacc agctcgtcgt
     3121 ccacataact ctcgccttaa tacagaaaaa tttcagcaga actttgcgct tgtcttgcct
     3181 gactggcagg ttggcgtgaa acgaatgctc aacgaattat ttacgactac agcaatttaa
     3241 tattttttgc atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa tgatgaaatg
     3301 aaaacgcgta aaggtattat tttagcgggt ggttctggta ctcgtcttta tcctgtaact
     3361 atggctgtca gtaaacagct gttaccgatt tatgataaac cgatgatcta ttatccgctc
     3421 tctacactga tgttagcggg tattcgcgat attctgatta tcagtacgcc acaggatacc
     3481 cctcgttttc aacaactgct gggtgatggt agccagtggg ggataaatct tcagtacaaa
     3541 gtgcaaccga gtccagatgg tcttgctcag gcatttatca tcggtgaaga gtttatcggt
     3601 ggtgatgatt gtgctttggt tctaggtgat aatatctttt acggtcacga tctgccgaag
     3661 ttaatggata ccgctgttaa caaagaaagt ggtgcaacgg tatttgccta tcacgttaat
     3721 gatcctgaac gctacggtgt cgttgagttt gataaaaacg gtacggcaat tagcctagaa
     3781 gaaaaaccgt tacaaccaaa aagtaattat gcagtaactg ggctttattt ctatgacaac
     3841 aacgttgtcg aaatggcgaa aaaccttaag ccttctgccc gtggtgaact ggaaattacc
     3901 gatattaacc gtatttacat ggaacaaggg cgcttgtctg ttgccatgat gggtcgtggt
     3961 tatgcatggc tggacacggg gacacatcaa agtcttattg aggcaagcaa cttcattgca
     4021 acaattgagg agcgtcaggg gctaaaagtt tcctgcccag aagaaattgc ttaccgtaaa
     4081 gagtttatcg atgctaatca gataaaagtg ttagctgaac cgttgaaaaa aaatgtgtat
     4141 ggtcagtatt tgctgaaaat gattaaggac tattaagaaa atgaacataa ttaaaacaga
     4201 tattccagac gtactgattt ttgaaccgaa agtattttat gatgaacgtg gtttcttttt
     4261 tgagagcttt aaccagaagg tttttgagga ggctgtagcc cacaaagttg aatttgtcca
     4321 agataatcat tcgaagtcta gtaaaggtgt tttacgcggg ttgcattatc agttagaacc
     4381 ttatgcgcaa ggaaagttgg tacgatgtgt tgttggtgaa gtttttgatg ttgcggttga
     4441 tattcgtaaa tcgtcaccta cctttggcaa atgggttgga gtgaatttgt ctgctgagaa
     4501 taagcgccag ttgtggatac ctgaaggatt tgcacatgga ttcataacat tgtcagatat
     4561 agcggaattt ctctataaaa caactagttt ttattctccg aaacacgaag tttgtattag
     4621 atttaatgac gaaaatattg gtattcattg gcccaatata actgataata cgttaatatc
     4681 tccaaaagat aaaaaaggtt taaattttaa ttcgttagtt tgattaagat tttctattta
     4741 tatatttaat gattgccccg caaattcact gacgcgtaga gaataagtta cactgacgac
     4801 tggacgcggt aatgaatgac gacgagtgca aagtaagaag aggaaacgca gcagaattat
     4861 tttcagggat acggcacatt gctattaata ttttgacgaa tgataaggta ttcaaggcag
     4921 ggttaagacg taagatgcga aaagcagcca tggacagaaa ctacctggca tcactccttg
     4981 cggggagctg cctttcgtaa tcttgccctg ctagcgtcag tccttgggga gagaaggctt
     5041 tcgtactctt tccttatcca gtaagcgagt ttttgaaatt attactttca ggttaaatca
     5101 taatgagtgg tgtaaaaaaa aagctgctaa aaaatttctt ttcgctttct atgttacagg
     5161 gattaaatta tttaattcca ataataacaa tgccttattt attccggact cttggacctt
     5221 attattttgg ggttttggca atagcaactg ccgctataaa ttatttagtg gttataactg
     5281 actatggttt taattttata gcgacaaaag atatatcagt aaatagagaa aataaggaaa
     5341 agcttataga aatttactct tctgtactta caattaaatt gttgctgtgt cttttatgtt
     5401 tttttatttt attattaatt ctttttttta gtaaacagga ttgttttttt atacaagtat
     5461 atatttttac atatggtatt gtgttagggc aaagcctatt ctccacttgg tttttccagg
     5521 gtgttgagga aatgaaatac attactctta ttaatgttgt ttttaaattg tccgcaactc
     5581 tatttatatt tattatcgtc cgaagcaaag atgatgtttt atttgttccg ataattagtt
     5641 cattgggtta tattctatca ggtcttgcat ctttttttat aattcagagg aaatatttaa
     5701 tcagttttca gccacaaaca ctaatgacta taaaagttta cttttcaaaa aattggaata
     5761 tgtttatttc taatttcttt actctattat atagaaactc aaatgtttta attcttggct
     5821 ttttttcaag tgcatcaatc gttgcaagtt atgcgattgc agaaaaaatc atcaaggcta
     5881 ttcaatccat acaaatgatt tttggtactg catgctatcc atatctggct aaaaaattca
     5941 ataatgatca tagttcgttt tatgaaatta gcaaaaaata ttggttaatt attttgcttg
     6001 cgtacttttt attaacctta attgtgtatt ttttatctcc agtggctatt tattattcaa
     6061 ctgggtatag tgattttatg gcaattcaat taatgaaaat aatgtctttc ctcattttat
     6121 tcggtggact agattacaca ttaggtgttt tagggctcat ccctatggga tattcaaaat
     6181 acttctctaa tagtgtagtt atcacgggca tattcagtat tacattgagt gcattacttt
     6241 caaaatatta tggcggttat ggggctagta tttctattgt tttatgtgag gtgttacttt
     6301 tagttgctct atctctaaaa ataaaatctc tatacttaag taggggaagg aaataaaatg
     6361 ccatatatag ttatatcgtt aagccctttt ttagctgctc caagtttttt atctaccctt
     6421 ggcactctgt cttttttttc attaattatt gcaaatttgt atttttattt taaaatgtcg
     6481 acatctgcgg tattacccag aacaataaca ccgatgatat gcatttcatt tttatttata
     6541 atatgcctta ttttttcagt tttatatcat gatgttgcat caaaaaccat aaactatttt
     6601 ttcttttacg ttttcatgat aagttgtatt attgcaataa gtttcatatc ctatacgaaa
     6661 tttatagatt taaaaaaaat aaataaacta acgttatata tactgatatt tctatttatt
     6721 ttcgcattca ttgaatatta ttatattatc acgaataatg gatatatctc atgggtacct
     6781 agatttgagc gaccagacta tgcgtcgaat tcattttctg gtttatatcg cattcgatta
     6841 tttacttatg agtcatccag tgcaggagcg ttactttatt cattgtatct gattttttta
     6901 tacaacatca aaatcacata tcaaaagaat atctttgtat tactaataag tttagcacta
     6961 cacctgatga ttttttcttc aacacaattt gtgtttttca tgattataat aatcgctcaa
     7021 tttttttcca gtaatctaat ttcaagaaaa gcaaagattt ttcttttaat tttagttctc
     7081 gtcggaatag tggttgcaag ccatgaagtt ggaatattaa taaattatat atcaaataaa
     7141 ctagatggat ttataacagg aaatgcatcg aatgcgtcag cttcagaacg aaataagcta
     7201 ttattgatag gggtaaatgc atttttagag aaccctatat ttgggggggg gttagcatca
     7261 ttttataatt atgtggatac tggattagtt aattttcatt tacagacatt acagcaattg
     7321 ggaatttttg gttatttact atttttaacc tttcttttat accctttgct attttttaaa
     7381 caatacacac tggaaataaa attattattt atactgacat ggttcactct tttcttttct
     7441 ggttctattt gggattctaa tttatattta ccatacttat tcatagcggg aaaatcaaat
     7501 gaaagataaa agctttataa tgctctgcac ccctgggtat attaaaaaaa ccgggtatta
     7561 tttccgagcc gaaagagata aagacgtatt acagagctta aactttaaag aaaagggcac
     7621 aatagttata aatagcttaa aaaagatcat aaatactgta ttgcttataa aaaaaaataa
     7681 aaatacctta tttatttgtg aaaacattgc ggcagcttta ccaatcgcgc tattctttct
     7741 catgtataaa aaaacaagtt taaaagtgat tttagtatat catggtagtt tggaggattt
     7801 aaaagcattt aaattttata agttaaaacg ttttatttat gagaaaatag aacgtattat
     7861 gattcataga gaaacaacag ttttgtttgt ttcaaatgaa atgaaagagt attataatat
     7921 taaatatggt gacttggaaa gttttcatgt gtcgccgaat atacctagcg atttattttt
     7981 tgaatccata aacgatgcta aaaataagga gaaaaatgaa ttaagaaacc tcctgagctt
     8041 acctgaagaa aaaaaaatag tatgttactg tgggaattct caatcttggc aaaaagttga
     8101 gttgcttaca aaaataataa attttaatac agatggcgat ttgtttttct tgatattgac
     8161 ccgagacact gattacttca taaaaaacat atctaaaagt aaagtaaata atacaattat
     8221 taaaactgtc ccaaacgatg aggttcctta ttatctggtt gcatcggata tgctttatat
     8281 attacgagat gataatgaaa ctaatagatt ttcctgccca acgaaacttg ttgaatatct
     8341 ttggtctcaa aagcctgttt taatttcaga aaatataggt gatttttctc aaaaaataaa
     8401 atgttataac tctggaatag tgctaaatag taatcattat aatgacattg atttaattac
     8461 ctctttaatt aaatgcttta atggggctgc ttttccagat gatgagctag acaactatac
     8521 tttcagcttt caacataatc tctttaaagc gattttatct gatatttagt ggaaaggatt
     8581 tttatgataa agatactatt tggaattgtt ttatattctt acacacctaa aaattctcca
     8641 acatatagtc acttaataaa caataacatt aatcctgatg atatatacct tcatgataat
     8701 agagaggaaa atataaaatt aagtaccgtt tataatgatt ttttaaattt tgcaaatgaa
     8761 aacagttatg attatctagt gattttagat gatgatacct tcctgtctga cgagtacctc
     8821 aaatcattac actcaaatct taattgttca gtccttattc ctaacttaac ctacgagcat
     8881 aaaataatca gccccttaaa attcaaaggt atacttagat tttgtatgag tggaattaga
     8941 cttcacttat caaaactcaa ttctcttaaa ttttctgaaa attattcact gtatggcatt
     9001 gatgattatt tggttatttt tttaataaag aataaaataa aagtaaaaat aagcggcgca
     9061 tatattgagc atgaactatc aactattcgt gggatacaag gagctaactc atactctgat
     9121 tttaagtgta ttgaacttct tcgcgcgcat atattaaact tcaaagaggc accacttttt
     9181 ttgaaaatct tttatttcaa gaaaatatca ttttttttat ttcctttatt taaaagacga
     9241 aatttcttag aacccatgaa aatatttttc tctcctgctc aacggtcaat gtatattcaa
     9301 gaaaaagatt aaatatgaaa ataatatttc tgtcttctta tgatcctcaa gataataaaa
     9361 gctggtcggg tactcctaaa aggatgctcg atgctttaaa aagtatggat gctgaaatta
     9421 taatcattgg cccatatgat tctaagataa aaaaaattct caagtatatg tcttttgcag
     9481 tgaagaaaat actcaaatat aattatgcac attctattct tgcatcttta gaatataaat
     9541 tattttttca atcagtaatc aaaaaacatt ctgacgctaa tttcattttt gctcctgctt
     9601 catcaactga gattgcttta ttaaatacca gtattcctat aatttattca tctgatgcca
     9661 catttgcact tatcgatagt tactatgata atttttcaaa tttgcttaag atatcaagaa
     9721 aagaatctaa cttcatagaa aaaaaagctc tagatgtcag caagttcata atttatccat
     9781 ctgtttgggc aaaagaatca gccattaatg attatttaat agataaagat aaaattaaag
     9841 ttataccttg gggagctaat tttgattata ttgaaaatac atttaatgaa aaaaaaatca
     9901 caaaatcagg aaaaattaaa attctttttg ttggggtgga ctggtacaga aaaggtggtg
     9961 atattgttta taaaacctgt gaaaatatta gagaaaagta taatttagat attgaattgc
    10021 ttatttgtgg ttgtacccct atcggggttg actctaacgt taattggata aagtgtttcg
    10081 gacggttaaa caaaaactta aaagaagatt tagatatgct tatagattta tattcaaatg
    10141 cacatctttt atttgttccc tcaagaaatg aatgttatgg aatggttttt tgtgaagcaa
    10201 gtgcttattc attgcctata atctcccata acacaggtgg aataggttct gtagtatctc
    10261 ataatgtaaa tggctatttg ctagatgtcg actctccttt acaggaatat gaagccaaaa
    10321 ttatgtatct agtaaataat cctgatatat accggttgtt ctctattaac tcaagaaata
    10381 tttatgaaaa aaaactgaat tggcgagtat gggtcaaaga tttattctca tatattaata
    10441 attattaaaa ataaaattta aaaattgcaa acattatttt cttggttttt tccctctagt
    10501 taaatagctt ttaaattttt aattactggc tatgtaggga aagagcactg tgtgccgtag
    10561 gcacaaagat gcaaaacgga acgttttgca tgctgggaaa gaatattaga agtacacttt
    10621 ttgtactaaa taattagcat tttatgttta aaaattgaga tattcctcat cacctgaagc
    10681 tgttttttta ttgcttatac atgatcaaat actccttaca taattaagga gaacaaaatg
    10741 gaacttaaaa atttgatgga acatatttct gttatccccg attacagaca agcctggaaa
    10801 gtgaacataa attatcgaat attctactgt tgactatgtg tgccgttatt actggtgcag
    10861 aaggttggga agatatagag gattttgggg aaacacatct cgattttttg aagcaatatg
    10921 gtgattttga aaatggtatt cctgttcacg ataccattgc cagatttgta tcctgcatcg
    10981 gtcctgccaa atttcacgag tgctgtatta actggatgca tgactgccat tcttcaaatg
    11041 ataaagacgt ctttgcgatt gatggaaaaa cgctccggca ctcttatgac aagagtcgac
    11101 gcaagggagc gattcatgtc cttattgcgt tctcaacaat gcacagtctg gtcatcggac
    11161 agatcaacac ggatgagaaa tctaatgaga ttacagctat cccagaactt cttaacatgc
    11221 tggatattaa aggaaaaatc atcacaactg atgcgattgg ttgccacaaa gatattgcag
    11281 agaagataca aaaacgggca ggtgattatt tattcgcggt aaatgaaacc caggggcggc
    11341 taagtaaagc ctttgatgaa aaatttccgc tgaaagaatt aaataatcca gagcatgact
    11401 gttactctat gattgaaaag attcccggca gacaacaaat ccgtcttcat attgattgcg
    11461 atgtccctga tgaacttatt gatttcacgt ttgaatggaa agggctgaac aatttatgca
    11521 tgtcagtctc ctttctgtcc ataataccag aacgtgcgat ggagccagaa ctgacgggca
    11581 gatattatat cacttctgct gatttaaccg cagagaagtt cgccacagca atcccaaatc
    11641 actggcacgt ggagaataag ttgcactggc gtctggacgt ggtaatgaat gaagacgact
    11701 gcaaaataag aagaggaaat gcagcagaat tattttcagg gatacggcac attgctatta
    11761 atattttgac gaatgataag gtattcaagg cagggttaag acgtaagatg cgaaaagcag
    11821 ccatggacag aaactacctg gcgtcagtcc ttgcggggag cgggctttcg taatcttgat
    11881 ctggtatagg tactttcggc cgtatcaatc ttattataaa tagtatatta cagtgaaagc
    11941 aaatatgaaa acaatggcaa gttctgcaaa tactagatgg tatttgtata attttagagt
    12001 caatacaatt agatgtcttg taaaagaagg ttaccgggtg ttagctatag cccctaaaga
    12061 tgattattca gagcagctcg aattattggg tagcgagttt attaatatat ttatagacca
    12121 gagtggcaaa aatcctataa aagatctgat tacgttattc aaattctatt ccatctttaa
    12181 acgagagaat gttaactgcg tccttaactt tacacccaaa aatactattt acagcacatt
    12241 agcagcatcg ctcgctaacg taaaagcaat taataatatt gctggtctgg gtcttttgtt
    12301 tattgaagag tgtataacat caaaaatagc tggtatgtta tataaattaa gtcaaagaag
    12361 agcttcaaaa atattttttc agaatgaaga agatagagtt ttttttctaa gtcaaaaaat
    12421 tgtcgatata tctatgacag atagattgcc tggctctgga gttgatctaa aacgttttta
    12481 ccactctcca tctccagatg atggggttgt acgatttttg ttagttgctc gtatgttata
    12541 cgataaaggt atagggcaat atatagaagc agcacgccat ttaaaatcta aatatggtaa
    12601 taatgtagag tttcgtttgc ttggtttttt agatgttaat aacccttctg cagttactaa
    12661 agaagacatg caatcatggg tgaatggggg gattgttatt taccttggca catctgacaa
    12721 tgtagaaaga gagattgcta aagccgattg tatggtattg ccatcttact acagagaggg
    12781 tatacctaaa tctctattag aatcaggtgc catgggtaaa ccaatagtaa caactaataa
    12841 cgttggttgt agagaaacgg tagatgatgg aattaatggt tttttatgca ttcctcgttc
    12901 ctcagaaagc cttattgaaa aacttgacct aattataaat atgtcgcatg aacagagagt
    12961 cgaaatgggc ttagcaagcc gtaaaaagat tgaatgtgaa tttaatgaag aaattgtgat
    13021 taaaaaatat ttagacgcta tagttcagtg tctggaaaat taatttagtg taattgttgt
    13081 gttaatatcc agtcatatac tttcttgtac gtaacatcta ataaagtaac tttataaaaa
    13141 acctatatat acattatgca gggaaagatc acgtaacgct gcttttttgt actaaataat
    13201 tagcatttta tgtttaaaaa ttgagatatt cctcatcacc tgaagctgtt ttttttattg
    13261 cttatacatg atcaaatact ccttacataa ttaaggagaa caaaatggaa cttaaaaaaa
    13321 tgatggaaca tatttctgtt atccccgatt acagacaagc ctggaaagtg gaacataaat
    13381 tatcggatat tctactgttg actatttgtg ccgttgttac tggtgcagaa ggctggaaag
    13441 atatagagga ttttggggaa acacatctcg attttttaaa gcaatatggt gattttgaaa
    13501 atggtatttc tgttcacgat accatagcca gatttgtatc ctgtatcagt cctgcaaaat
    13561 ttcacgagtg ctttattaac tggatgcgtg actgccattc ttcagatgat aaagacgtca
    13621 ttgcaattga tggaaaaacg ctccggcact cttatgacaa gagtcgccgc aggggagcga
    13681 ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt catcggacag atcaagacgg
    13741 atgagaaatc taatgagatt acagctatcc cagaacttct taacatgctg gatattaaag
    13801 gaaaaatcat cacaactgat gcgattggtt gccagaaaga tattgcagag aagatacaaa
    13861 aacagggagg tgattattta ttcgcggtaa aaggaaacca ggagcagcta aataaagcct
    13921 ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt tacgcaatga
    13981 gtgaaaagag tcacagcaga gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg
    14041 aacttattga tttcacgttt gaatggaaag ggctgaagaa attatgcatg tcagtctcct
    14101 ttcggtccat aatagcagaa caaaagaaag agccagaaat gacggtcaga tattatatca
    14161 gctctgctga tttaaccgca gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg
    14221 agaataagct gcactggcgt ctggacgtgg taatgaatga agacgactgc aaaataagaa
    14281 gaggaaatgc agtagaatta ttttcaggga tacggcacat tgctattaat attttgacga
    14341 atgataaggt attcaaggca gggttaagat gtaagatgcg aaaagcagcc atggacagaa
    14401 actaccttga gtcattcctt gtggggagag ggggctttcg taatctgctc tgttattcgg
    14461 ataaggtaat gctattcatt ttgtcgatga ttgggttaac atctacacca tatttaagcc
    14521 gcaaactcgg cgtgaccacc ccctgacagg agtaaacaat gtccaagcaa cagatcggcg
    14581 tcgtcggtat ggcagtg
//
