LOCUS       AB812067               25001 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 10B-1.
ACCESSION   AB812067
VERSION     AB812067.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 25001)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..25001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10B-1"
                     /db_xref="taxon:562"
                     /note="serogroup:O162"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01819.1"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNINVMQESSVGSALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPQRRE"
     gene            1068..1973
                     /gene="galF"
     CDS             1068..1973
                     /gene="galF"
                     /note="O162_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01820.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVI
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVIIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLGK"
     CDS             2416..3432
                     /note="O162_02"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ01821.1"
                     /translation="MTILVTGGAGYIGSHTVLQLIEGGYDVVVLDNLSNSSQESLHRV
                     GSLVSKNIPFIKADINDKQKLEEVFKNYDIDAVIHFAGLKSVGESVKKPLEYYINNVS
                     GTLTLLEVMKQANVYKFIFSSSATVYGNHSPVPNLEEYPIGNASCPYGTTKVMLEQVL
                     SDYAKSDDKLKIIALRYFNPTGAHPSGLIGEDPNGIPNNLVPYISQVAIGKLEKLSVY
                     GGDYETIDGTGVRDFIHVVDLAAGHVSALRYIDKLQGYDIFNLGTGTGVSVLQVIDAF
                     EKASNQKVNYEIVARREGDIGSSWADVSKAERILGWKSKYNIEDMMVHTWNWQKQNPN
                     GYGS"
     CDS             3466..5682
                     /note="O162_03"
                     /codon_start=1
                     /gene="werR"
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01822.1"
                     /translation="MNLFFIVDKLDWAFGRIAQNIATELSSFNVKIVCTDDFKTVADF
                     AMHMDKNVKEKSILHFFWRDYLMEVMNFTQRNPQYKEILIRNIITTHIPDHLFTYDDI
                     ANYERRIPLMNFVDGYFVTSGKLKELYSELSDFPPPHSVIYDNPEIPMSEVKSYDNHD
                     RKKIVWIGNSKWGEYLGYQDYKGLNTIVLPALDKLKKSGYTFDYIEFDSSKGKTDREV
                     ILKTLSDADILLISSKCEGTPLPLLEAMANSCAVVSTDVGIANEVLPEKQLQYIVSRD
                     AEAFYYSLKGLLDDQRGLEELQRMNYIAYRQQFVDDSLIGDKWALFFKDMCSKSQNEF
                     KNIFLREQKIPSFKERLISKAIYNLSRLALRLNLMNTLKQYSLLRKIYYKVAGTTTNQ
                     VDYDLLDEFYSNAISDKEVLAFYSAYWSGVATSTASFFKDDSIQYPYYSYEFPQVSNH
                     VYLNRLAEKLAESKTLKSVIMSGGTQLQMELAKKIKALSPKIKIFFAWHGSPAQWVDS
                     SQYSTFDGWYELYRERIADGVISFKPELDEILNAYGIRAFSVSNYAVSGSSFSKKLAA
                     PDSANFRVGLFAAMFSWYKNPFPQLLAIGSLKGCELVTNLHLEHNMKWVTSNIKLTTL
                     SENLNNKRFIELLSRLHVICYVTNTECSPMIALESASVGTPCIVGPAGNIYKGFPELE
                     LYLVEKEVDNPTAIRNRLQLVRDNYEEITVLLNAFVLEYNSRLELIKEKMYKELKQ"
     gene            5679..6458
                     /gene="wzm"
     CDS             5679..6458
                     /gene="wzm"
                     /note="O162_04"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="BAQ01823.1"
                     /translation="MKESLTDIYLSIKSWHLWTTLGWLELRQRYKRSIVGPFWITLSM
                     AVLIIALGVIYGELFKLEVKEYLPMLAIGLVFWSFISGVINEGCVTFISSSSFISQLP
                     TKKFIYIMQMLWRNFMMLCHNIIIVIGVLIIFNVFSLRNFPLFILGFMIVAVNLIWIA
                     TLFAMISVRFRDFPQMVMSFMQVVFYITPILFSGTMLAKYSLLLKLNPFAWFIEIIRS
                     PLVGKMPELYCYYNSIIFACVGTIISLITFAKCRNRIAYWV"
     gene            6465..7217
                     /gene="wzt"
     CDS             6465..7217
                     /gene="wzt"
                     /note="O162_05"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="BAQ01824.1"
                     /translation="MSHLIDLKDVGVEFPIYDAKSRSFKSQVANLTRGVISSKKESST
                     VTVKSLDGLTFSLKEGDKLGLIGNNGAGKSTLLKLLAGIYEPTSGTIKRKGKIVPLLD
                     IALGMDDDSTGYQNIKLRGLLLGMTNAEIESKIEEIACFTELGDYLNLPIRVYSTGMR
                     VRLAFAVSTSVDPDVLLLDEIIGTGDASFLNKAKKRFEELQERAKVVVLSSHDNNVIL
                     KTCNKVLWLEGGKPKMIGDPEEVLNKYMDSVI"
     CDS             7234..8313
                     /note="O162_06"
                     /gene="wekV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01825.1"
                     /translation="MKIDYVITGMNVGGAEMQVVELLLELKRCGHSVRLISLTPPVDL
                     IDRLIRNNIPVVSLGMKSKLGLPFAAFKLARLMRQNSPDIVHSHMFHANILVRLVRIF
                     LVDSTSIICTAHSIREGGSARDWLYRLTNKLSDLNTVVSDAARLRFINDKVFPSEKTL
                     TVYNCINIDHFRIIDRQSRKRFRWVTVGRLVKVKNQLLILQAMMKLPLSELIIIGEGE
                     ERLSLQTFIDQNSLSERVTLLGKKDNVVDYYHDSDAFVLASDYEGFALVVAEAMACGL
                     PVVATNCGGPAEIIGNGQDFGITISVNDVHHLTAAMQQIESLDVEQRRGSGYRARERV
                     IEKFSTKKIVSQWEDIYLRIQKNRI"
     CDS             8323..9477
                     /note="O162_07"
                     /gene="werS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01826.1"
                     /translation="MKIALIGTTAESLIQFRKELINLLHKNGHVVYAFAIDYDDVTKE
                     KVKSFGAIPVHYCFSRTGLNPFSDIINTYKLYKILKSLKLDLVFSYFSKPAIFGTLAA
                     KFARIQKRYAMLEGLGFFFTNSGGQVSLRNKLLKKILVSLYKLSFRHIESLILLNADD
                     KRDLLVNAKIQVKKVHILGGIGLNMSDYPYCPPPVDPVSFIFVARFLQEKGVYEFIEA
                     AQVVKKKFPETHFCMLGHLDLHNPGSLTTERLNDLKCNNIIELPGHVDNVQEWLAKAS
                     VFVLPSWREGFPRSTQEAMAMGRAVITSDVPGCRDTVVDGVNGFLIKPRSSHALAEKM
                     LCFLHQPELITQMGNASHQIALQQFDSYIVNSKLMKILRVDDENTKSNDC"
     CDS             9526..10674
                     /note="O162_08"
                     /gene="wbxB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative methyltransferase"
                     /protein_id="BAQ01827.1"
                     /translation="MERLNLSEKPAHSLQEAAIHCARYANILHLVKDKVVLDIACGEG
                     YGSALLMKAGAKRVVGVDISQESIEHAKKLFGGYNVEFIVSDACTISERYGEDFFDMV
                     VSIETIEHINTPEVFLSAIKKTAKENAIFYITCPNDYWYYPNDEQSNPYHVRKYTFQE
                     FTELSTVILGKNVQWAYGCSAFGFCTVSANNRGFEKIGSSWMEISDSENSITVLNTDI
                     DAVSENNCSFFVGLWNAPETNFSNSTFPISMNAYSRMTEEFESNVSLVLREEQAENKK
                     AISILKQDMKKLTMDLRKSNLLLFALKIENDALRTNISELQQQLARKIAMYDEVTNTN
                     NNLVTTNSSLMERVSQMEIPYYRYVRLSALLPDFLKKMILKIMRLIRK"
     CDS             10677..12758
                     /note="O162_09"
                     /gene="werT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01828.1"
                     /translation="MSARLKLLPGVKSTEYVYSDLHALLDSNAESSFGPNIGSDGSNL
                     TITCLSMSRVFLTEKLVNSIYQHIPNFKGDILIVDNGSTLEELSVLQTLSDRIPLHIR
                     VIELGNNFGVSGGRNKALEHIKTEWAMFLDNDIYFINNPFPRLQKDISKLGCHFINMP
                     LLDSDGETLFANGGNIFLHIDNDSVHVGAGSACVQEKITTYDGEGFLSTFLFGGASVI
                     KIDSLKSLGKYDENMFIGFEDIDLSLRLYQSGMKVGTCGAISLIHDHPKPSSNKDKDY
                     EKIRFTKSILKESADYLENKWGMVFWSAPVDDWLEEKQRSFELITQKESNYSQIDDNK
                     QNVICDFPVAKPRIALIIDTENWAFSNIANQIVNHLSDSYDFTIIPTEIIDNISQVIM
                     MTKNHDITHFFWRESLRLIYDECYINYNRAIGFDELSFKKQYLDGRIITSSVYDHLFL
                     DEQAIKLRKTFYNDLITAYTVSSSKLYDIYSSIAEYPNPSVLAEDGVDLKLFYPLDLE
                     RFDNIESRTLRVGWAGNSKWAGEVEDFKGYHSLLKPAVEQLQSEGLNIELVLADRQLG
                     FIPHDEMVNYYSQIDVYVCPSKIEGTPNPVLESMACGVPVISTDVGVVNDAFGDMQKE
                     WILPVRSKDVLIDKLRDFYNRRNTVVKCLSSENLQQIKKWDWKVKSENFRIFFDKILE
                     SKKHSKINTEM"
     gene            12848..14254
                     /gene="gnd"
     CDS             12848..14254
                     /gene="gnd"
                     /note="O162_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01829.1"
                     /translation="MSKQQIGVIGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAVIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AIAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELADT
                     FTKWNEGELSSYLIDITKDIFTQKDEEGKFLIDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQLAGDKVEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYKWDLQYGEIAKIFRAGCIIRAQFLQKITDAYANNDKIENLLLDPY
                     FKNIADEYQQALRDVVTYAVQNGIPVPTFGAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLN"
     CDS             14395..15510
                     /note="O162_11"
                     /gene="wbxC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase"
                     /protein_id="BAQ01830.1"
                     /translation="MRKNIIIVGGGFFGMYLAEQFSLLGHGVKLFEKENNYMSRASYN
                     NQARVHNGYHYPRSILTALRSRISFPRFVDEFRDCVDSDFDKYYLISEILGKVSASQF
                     RMFCDRIGADCDYAPAKIQNMVNPKMINACFTVKEFAFDAFKLRDIMLKRLDSSNAEF
                     YCGYSVDKIEQLENGIKTLIREVSTGEVQEYVADHVFNCTYSHLNYLLDNSGIELIPL
                     KHEMTEMCLVDVPEVCKNAGFTIMCGPFFSVMPFPSVGLHSFSHVRYTPHYEWYDKPG
                     EKYLDSHDKFDSDTKNSAWQYMIRDASRYIPVLNECRYERSLWEVKTLLPRSETDDSR
                     PILFRPNHCIQGFHCIMGGKIDNVYDAFEAIKSMNVI"
     CDS             15532..16542
                     /note="O162_12"
                     /gene="werU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01831.1"
                     /translation="MKSESFVSVVLSTHDLSSELTNKLKQIQSLLDDNYTDYEILIVS
                     KGPYRDVPRTSAIDNILKTIPSVRFLQLSGCVHDDVVRAAGLENAIGDFVVLYDHQTD
                     PVYVIHQAVEECKSGYDVVVGVSTQSLPCAYRIMRDTASHILKAIDYHIPKNSTGLRC
                     LSRRAVNAVTDTGRFHHQFYMRIQKTGYPVSELTYQPVSETHIKRSITYGFRNLVRLM
                     VFNSSRPLRWMSIVGTVGSLSALLFAVYSILVNLINGHVVEGWTTTVLFMSTLFMLQF
                     IMLAFFGEYLSRLLDDRSEQAAYSVVFEKNSAVMINKDRVNVLNDATVEEANLVQTGR
                     DR"
     CDS             16557..17339
                     /note="O162_13"
                     /gene="wbxD"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01832.1"
                     /translation="MTKALIGYTGFVGTTLLRQTKFDDFYRSTNINDIKGKKFDLVVC
                     AGAPAKKWIANADPKGDQETIDSLIECLNNIECKTLILISTVDVFKSPNGVDENSVID
                     TQGLHAYGLNRYRLERFVAKRFQQHLIVRLPGLVGPGLRKNIIFDFLNDNNVEKIESR
                     SIFQFYPMVNLWFDIKVALENKINLIHLTAAPVSVAEIALDGFGINFDMQQAAQPVFY
                     DMRSVYGALFGGSAHYQYSVKDTLQAVRHYAQSEPKKRAGIQ"
     CDS             17340..18152
                     /note="O162_14"
                     /gene="wbxE"
                     /codon_start=1
                     /transl_table=11
                     /product="putative xylose isomerase"
                     /protein_id="BAQ01833.1"
                     /translation="MRVSISNLAWEVTEDVEVANLLSAFNIDAIDIAPGKYFPEPTNA
                     TIADIQTVKEWWQERGIDIIGMQALLFGTTGLNVFGSKSVQKKLLNHLVAVCRIGAGL
                     GATRLVFGSPKNRDCSGLSVSQVQNISQKFFRQLGDIAADHGVIVCLEPNPSVYGANF
                     MTTSFETCRVVHEIDHPAIKMQLDTGAVIINQEDVSDVVTQAKDVIGHVHISEAQLAP
                     PGDSYFRHDDIAAVILSQLPAHTATIEMLATRNEPHLVSIRRALQVVTSLYR"
     CDS             18455..19339
                     /note="O162_15"
                     /gene="werV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01834.1"
                     /translation="MDDNCWRGVCHRRCCINYVKSCLIMDLSMKHEKNWEVPSYDVTL
                     WTEKKHDACVVIPVINEGSRIISLLTKMSNLKINNIADIIIVDGGSTDGSLEKDRLLS
                     LGVSGLVLKIGPGKLSAQLRCGYAFVLEQGYKNIITIDGNDKDDPSPIPEFLNALEKG
                     YDFVQASRFINGGVAVNTPKLRDFAIKYIHAPALSIASGFHWTDTTQGFRAYSSKLLL
                     DGRVAPFRNIFMTYELLAYLSYIAPKLGFRCIELPTSRIYPNGDVPTKISGFNGNLSV
                     LIILFKTCFGRYNSNRCN"
     CDS             19392..20762
                     /note="O162_16"
                     /gene="wexK"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01835.1"
                     /translation="MGVVMNIFGFDHERVKIEVVLLIICVGGMLCSIYAFYPGWMSPD
                     SIFQYNNAVTGYYNSWHPVIMAWWWRVLGYITDGPGPFLVQNLMLFWIGFYFIAKTLS
                     RYIGWGSIVVLLLAATPSVLIIMAQLWKDAVFTSLAIFGLGIIFRSIDTGRFYKSVFL
                     LFVFVLSLAVGCKPNGLFVAIILIGWWFIANPKVSTKRSKLILSLTSVFLVMAIPFSI
                     SKCLPVYKISPIQYIQSYDLLGISVIENKTLLPDYITKKVGLSKDNAREFYFPGGNNL
                     MFYNSPAGNIATTDPEYLKDLQSRWISAIKDYPMAYIKVRFDNFIELLRWGAIHPASV
                     AADVIVNNPWGYVFIPNKLSDNYIDSIDKYPFLYFPWIYLVTSALLFLLTKVLPRELR
                     FFNVVLVLVVFSFSLPHFFIAPAADYRYLHFSVVCAVVQLGMFLGFFFMRLYKRVYFG
                     AIKLLG"
     gene            21085..22251
                     /gene="ugd"
     CDS             21085..22251
                     /gene="ugd"
                     /note="O162_17"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01836.1"
                     /translation="MKITISGTGYVGLSNGILIAQSHEVVALDIIQTKIEMLNQKQSP
                     IVDKEIEEYLATRSLNFRATTDKCDAYLDADYVIIATPTDYDPKTNYFNTSSVESVIR
                     DVVAINPDAVMVIKSTIPVGFTNSIKEKLGTENIFFSPEFLREGKALYDNLYPSRIVI
                     GERSERAERFAALLQEGALKKDIPTLFTDSTEAEAIKLFANTYLALRIAYFNELDSYA
                     ETLGLNSLQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLSNYESVPNNLIGAI
                     VEANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGITVII
                     YEPVMQEDEFFNSRVVRDFEAFKLEADVIISNRMAEELVDVGDKVYTRDLFGND"
     CDS             22372..22842
                     /note="O162_18; transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             23098..23733
                     /note="O162_19; transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     gene            complement(24067..24678)
                     /gene="hisI"
     CDS             complement(24067..24678)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01837.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQNLDLTTVIENLRKRHQ"
BASE COUNT     7348 a   4488 c   5556 g   7609 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaac
       61 tgtaagttca gcgacttaca aggcgacgcc attgaatgga acgtggcgat taacgacagt
      121 gatattttga tatctgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac
      181 tggggcattg gcataggtct tgcgggaagc acttatgata acaactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttatccgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggcat tgataacgcg acagttgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataac
      541 acccatcttg cttataaatt gcgcggcatc caaatctccg ccgggaatgc tgtctccttt
      601 gtggcactga ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
      661 catcttttta tgcgtaatat caatgtgatg caggaatcct cagttggatc agcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg
      781 ctgtcacttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc gcaacggaga
      901 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag gtaattgatg
     1021 ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg gatgaaataa
     1081 tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg ttgcctgcca
     1141 ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa accaatgatt cagtacattg
     1201 ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcac gcgtccaaga
     1261 atgcggtcga aaaccacttc gacacctctt atgaattaga atctctcctt gagcagcgcg
     1321 tgaagcgtca actgcttgcg gaagtgcagt ccatctgtcc accgggcgtg atcattatga
     1381 acgtgcgtca gggcgaacct ttaggtttgg gccactccat tttatgtgca cgacctgcta
     1441 ttggtgacaa cccgtttgtc gtggtgctgc cggacgttat tatcgatgac gccagtgccg
     1501 atccgctgcg ttacaacctg gctgcaatga ttgcgcgctt taacgaaacg ggccgcagcc
     1561 aggtcctggc aaaacgtatg ccgggcgatc tctctgaata ctccgtcatc cagactaaag
     1621 aaccgctgga tcgtgaaggc aaagtcagcc gcattgttga atttatcgaa aaaccggatc
     1681 agccgcagac cctggactca gacatcatgg ccgttggtcg ctatgtcctt tctgccgata
     1741 tttggccgga gctggaacgt actcaacccg gtgcatgggg acgtatacaa ctgactgatg
     1801 ccattgcaga actggcgaaa aaacagtccg ttgatgcaat gctgatgacc ggcgacagct
     1861 atgactgcgg taaaaaaatg ggctatatgc aggcgtttgt gaagtatggg ctacgtaacc
     1921 tgaaggaagg ggcgaagttc cgcaaaggta ttgagaagtt gctcgggaaa taatcaagac
     1981 aacagtttat ttgaattggc aatacttcat attaaatggc aggagaagtc tcgtggtgct
     2041 atcggtaggc agcatgactc tactgccttt tttgtgataa aaatcaagca gtaaaaaacc
     2101 aatattctac cttttcttgt agcaaaaatc gatttttcct tgtttcttgg tgagattaat
     2161 acgacaatat ctggaatgtt ctggggctaa atgtgtcaaa atgtaatcga taattggtag
     2221 attgagcccc gcaaaaattg gagtgtatag tatgctggta gctgttgagc caggggcggt
     2281 agcgtgcctg aataaattaa catttcggtg ttaaaataag gtaacgttat gttaaaggct
     2341 aacttacaac aaatataaga ccaaataatt gaatacgtaa ttagcataaa agattaatac
     2401 aatttcggag acgtcatgac tatacttgta acaggcggcg ctggttatat tggttcacat
     2461 accgttctac aattaattga gggcgggtat gatgttgttg tgcttgataa cctgagtaat
     2521 tcatcccaag agtcactaca tcgcgttggg tctttggttt cgaaaaatat tccattcatt
     2581 aaggctgata tcaacgataa acaaaagctt gaagaagttt ttaaaaacta cgatattgat
     2641 gcagtgattc attttgcagg tttaaaatct gttggtgaat cagtaaagaa accactggaa
     2701 tattacatta ataatgtcag tggaaccctt accctgcttg aagttatgaa gcaagcaaat
     2761 gtttacaaat tcatttttag ttcgtctgcg actgtttatg gtaatcattc tccggttccc
     2821 aatctggagg aatatcctat tggaaatgct tcatgcccct atggaactac taaagtcatg
     2881 ctggagcagg ttttatcaga ctatgcgaag tcagatgata aattaaaaat tattgctctg
     2941 cgttatttta atcctacagg tgcgcatcct agtggtctta ttggggagga cccgaacggc
     3001 attccaaaca atcttgttcc ttatatttct caggttgcta ttggcaaatt agaaaaactg
     3061 tccgtttatg gtggcgatta tgaaactatt gatggaacag gagtacgcga ctttatccat
     3121 gtggtagatc ttgccgcagg ccatgttagt gctttaaggt atattgataa attacaagga
     3181 tatgacattt ttaatcttgg tactggcact ggtgtatcag ttttacaagt aatcgatgcc
     3241 tttgaaaaag caagtaatca gaaagttaat tatgaaattg tcgccagacg tgagggcgat
     3301 ataggatctt cttgggctga tgtatctaaa gcagaaagga ttttaggctg gaaatccaag
     3361 tacaatattg aagatatgat ggtccatacc tggaattggc aaaaacaaaa ccctaatggc
     3421 tatggaagtt agtacctatt gatttaatat tataatggcg tatcaatgaa tttatttttc
     3481 atagtcgata agttggattg ggcattcggt agaatagctc aaaatatagc aacagaatta
     3541 agtagcttta atgtaaaaat agtttgcacc gatgatttta aaactgtggc agactttgct
     3601 atgcacatgg ataaaaacgt aaaagaaaag agtatattac acttcttttg gcgtgattat
     3661 cttatggaag tgatgaactt cactcagcgg aatccccaat ataaagaaat tttgatcaga
     3721 aatattatca caacgcatat tcctgatcat ctttttactt atgatgatat cgcaaactat
     3781 gagagaagaa ttcctcttat gaattttgtg gatgggtatt ttgttacatc cggaaaattg
     3841 aaagagctat attctgaact gtctgatttt ccgcctcctc atagcgttat ctatgataat
     3901 ccagaaattc ctatgagcga ggtaaaaagc tatgataatc atgatagaaa aaaaatcgta
     3961 tggattggca atagtaaatg gggagaatac ctcggttacc aagattataa aggcctgaat
     4021 acaatagttt tgccggctct agataaatta aaaaaatctg gatatacatt tgattatatt
     4081 gagtttgata gttctaaggg caagaccgat cgtgaggtaa ttcttaaaac actttctgat
     4141 gcagatattc tactgatcag ttcgaaatgt gaaggtacac cattgccact tcttgaagca
     4201 atggcaaaca gttgtgctgt tgtgtcgaca gatgttggta tagcaaatga agtattacct
     4261 gaaaagcagt tacagtatat tgtgtctcgg gatgcagagg cattttatta ttcattgaaa
     4321 ggtcttcttg acgatcaacg cggtcttgaa gagttacaaa gaatgaatta tatagcgtat
     4381 cgtcagcaat ttgtagatga tagccttata ggtgacaaat gggcattgtt cttcaaagat
     4441 atgtgttcaa aatcgcaaaa tgaatttaag aatatattcc ttcgcgagca gaaaattcca
     4501 tcatttaaag aacgattgat atcaaaagct atttataatc taagcagact agctcttcgt
     4561 ttgaatttga tgaatacgct aaaacaatat agcctgctac gaaaaatata ttataaggtt
     4621 gcaggaacaa ctacaaatca agttgattat gatttgttgg atgaatttta tagtaatgca
     4681 atatcagata aagaagtatt agcattttac tctgcatatt ggtctggtgt tgccacatca
     4741 acagctagtt tctttaaaga tgatagtatc caatatccat attattcata tgagttccca
     4801 caggtttcta atcatgtcta tctaaacaga ttagcagaaa aacttgctga atctaaaaca
     4861 ctaaaaagtg tgatcatgag cggtggaaca caattacaaa tggaacttgc caaaaaaatt
     4921 aaggcattaa gtccgaaaat aaaaatattt tttgcatggc atggttcacc tgctcagtgg
     4981 gttgactcct cgcaatattc aacgtttgat ggatggtatg aactttaccg agagcgaatt
     5041 gctgatgggg tcatctcgtt taagcctgag ctagatgaaa tactcaatgc ttacggtatt
     5101 cgtgcattct cagtatcgaa ttatgctgta tcaggctcct cgttttctaa aaaattagct
     5161 gcacctgatt ctgccaattt ccgcgtaggt ttgttcgctg caatgttcag ttggtataaa
     5221 aatccatttc ctcaattact agcaattggt tcattaaaag gatgtgagct agttacaaac
     5281 ttgcatcttg aacataatat gaagtgggtt acttcaaata tcaagcttac gacattatca
     5341 gaaaatttaa ataataagcg gtttatcgaa ctgctatctc gtttacatgt tatatgctat
     5401 gtaacgaata cggaatgttc gccaatgata gcgttggaat ctgcaagtgt cggaactcca
     5461 tgcattgtgg ggccagctgg aaacatatat aaaggttttc ctgagttaga attgtatttg
     5521 gttgaaaaag aggttgataa tccaactgca atcaggaatc ggttacaatt agttcgtgat
     5581 aattacgagg aaattacagt tttgcttaat gcatttgttt tggaatacaa ttcccggcta
     5641 gaattaatta aagaaaagat gtataaagag ttgaagcaat gaaagaatca ctaacagata
     5701 tatatttgtc aataaaatcg tggcatcttt ggaccacatt aggttggtta gagttaagac
     5761 agagatataa aagatccatt gttggaccat tttggattac actaagtatg gcggtcctta
     5821 ttattgcctt aggggtgatt tatggagaac tatttaaact agaagttaaa gaatatcttc
     5881 cgatgcttgc aattggtttg gttttctgga gctttattag tggcgttata aatgaaggat
     5941 gtgttacttt catatcgtcc agctccttta taagtcaatt gccaactaaa aaattcatat
     6001 atattatgca aatgctatgg cgaaatttta tgatgctttg ccataatata ataatagtga
     6061 ttggtgtgtt gattatattc aacgtttttt cgttgaggaa tttccctctt tttatacttg
     6121 gctttatgat cgtagctgtt aacttgatct ggattgcaac tttgttcgcg atgatttctg
     6181 tacgttttag agattttccg caaatggtca tgagctttat gcaggttgtc ttttacataa
     6241 ctccaatatt attttctgga acaatgctgg ctaaatatag tttactgctg aagttaaatc
     6301 catttgcttg gtttattgag attattcgat cgcctttagt gggcaaaatg ccagagctat
     6361 actgctatta taattccatt atctttgcat gcgtaggaac aattatctct ctaattacat
     6421 ttgcaaaatg tagaaaccgc attgcatatt gggtgtaaat tattatgtct catttgattg
     6481 acttgaaaga tgtgggtgtt gaatttccca tttacgacgc taaaagtaga tcgtttaaat
     6541 cgcaggtggc aaatttaacc cgtggtgtaa tttcttcaaa aaaagaaagc agcacagtta
     6601 cggttaaatc attggacggt ttgacgttta gtctgaaaga aggtgataaa ctcggactta
     6661 ttggaaataa tggtgcggga aaatctacgc tactgaaact cttggctggt atttatgaac
     6721 caacatccgg cacaattaaa cgaaagggta agatagttcc tctgttggac atcgctctag
     6781 ggatggatga tgattccaca ggataccaaa atataaagct acgtggttta ttactaggta
     6841 tgacgaatgc agaaatcgag tccaaaatag aagagatcgc ttgttttact gaactaggtg
     6901 attatctgaa tctaccaatt cgtgtctact ctaccggcat gcgcgtgaga ttagcattcg
     6961 cagtatcaac ttcagttgat ccagatgtac ttctactcga cgaaattatt ggtactggcg
     7021 atgcttcctt cttaaataaa gcaaagaaaa gattcgagga attgcaggag agggcaaagg
     7081 tagttgtgtt gtcttcgcat gacaataatg tcattctgaa aacttgtaat aaagtcctgt
     7141 ggttagaggg cggtaaacca aagatgattg gagatccgga agaagtacta aataaatata
     7201 tggatagtgt gatttaaaaa acaggtggta tgagtgaaaa ttgattatgt cattacaggc
     7261 atgaatgtcg gaggcgcgga aatgcaggta gtagaactgc ttctcgagct taaacggtgt
     7321 gggcattctg ttcggttaat ttcgcttaca ccaccagttg atttgatcga taggctaatt
     7381 agaaataaca tccctgttgt ttcattgggg atgaagtcca aattaggttt accatttgct
     7441 gcatttaaat tagccaggtt gatgagacag aattctccag atattgtgca ttcgcatatg
     7501 tttcatgcca atatcctagt acgtcttgta agaatatttt tggtggatag tacatctatt
     7561 atttgtactg cgcacagcat tcgtgaaggt ggcagtgcta gagactggct ttatcgtttg
     7621 actaataaat taagtgactt gaatacagtc gtaagcgatg cggcacggct gcggtttatt
     7681 aacgacaaag tatttccatc agagaaaaca cttactgttt ataattgtat caatattgat
     7741 cattttcgta tcattgatag acaaagcagg aaacgatttc gctgggtaac cgttgggcgg
     7801 cttgtaaaag taaaaaatca acttttgatt ttgcaagcga tgatgaagtt gccacttagt
     7861 gaactcatca taattggaga aggcgaggaa cgactctcat tacaaacatt tatcgatcaa
     7921 aattctctaa gcgagcgagt tactctcttg ggtaaaaaag ataatgttgt tgattattat
     7981 catgattctg atgcttttgt gctggcctca gactatgaag gatttgcact cgtcgtggcc
     8041 gaagctatgg catgcggatt gccagttgtc gcaacaaact gtggtgggcc tgctgaaatt
     8101 attggaaatg gacaggattt tggcataaca atttcggtta atgatgtaca tcacttaacc
     8161 gctgcgatgc aacaaattga atcattagat gttgaacagc gtagaggaag tggctatcgt
     8221 gctcgggaaa gagtcattga gaaattttct actaaaaaaa tcgtatctca atgggaagat
     8281 atctacctgc gcatacagaa aaacagaatt tagaattcac ctatgaaaat agcattaatc
     8341 ggtactacag cagaaagtct aatccaattc agaaaagaac tgattaattt attacataaa
     8401 aatggccatg ttgtgtatgc gtttgcaatt gattatgatg atgtaacaaa agaaaaagtt
     8461 aaatcatttg gtgctatacc agtacattac tgtttcagtc gaacagggct aaatccattt
     8521 tctgatatta tcaataccta taaattatat aagattctaa aatcacttaa gttggacttg
     8581 gtattttcat atttttcaaa gccagccatt tttggtactt tagcagcaaa atttgcaaga
     8641 atacagaagc gttatgcgat gcttgaaggg cttggttttt tctttaccaa ttctggaggg
     8701 caggtttcat tacggaataa attactaaag aaaatattag tatcacttta caaattaagt
     8761 ttcagacata ttgaaagtct tatcttgctt aatgcggatg ataagcgaga ccttttagtt
     8821 aatgcaaaaa ttcaagtcaa aaaggttcat atattagggg ggattggatt aaatatgtct
     8881 gattacccct actgcccacc gcctgttgat ccagtgtctt tcatttttgt cgctagattt
     8941 ttacaagaaa agggagttta tgaatttatt gaggctgcac aagtagtcaa aaagaaattt
     9001 cctgaaactc atttttgcat gctgggtcat ctggatctcc ataatccggg gagcttaact
     9061 acagagcggc ttaatgattt gaagtgtaat aacatcattg agttacctgg ccacgttgat
     9121 aacgtgcagg agtggcttgc taaagcgagt gtatttgtat tgccttcctg gcgtgaaggg
     9181 tttccgagga gtacacagga ggcgatggca atgggacgtg ctgtcataac cagcgatgtg
     9241 ccaggctgcc gagatacagt agttgatggt gttaatggat ttttgattaa gccacgttct
     9301 tctcatgcgt tagcggaaaa aatgctctgt tttttacatc aacctgagct aattacccaa
     9361 atggggaatg caagtcacca aatagcttta caacaatttg actcatatat agttaattca
     9421 aaacttatga aaatactgcg cgtagatgat gaaaatacaa agtctaatga ctgttaaatc
     9481 atataaataa aacgttataa taaatacaat ttagggggga atattatgga aagattaaat
     9541 ttgagtgaaa aacctgctca ctctttacaa gaagcggcta ttcattgtgc cagatatgct
     9601 aatatcttac atcttgttaa agataaagta gttctcgaca ttgcctgcgg cgaaggatat
     9661 ggctcagcac tattaatgaa agctggtgct aaacgagttg tgggggtgga catctctcaa
     9721 gagtcaattg agcacgcaaa gaaacttttt gggggatata atgttgagtt tattgtaagt
     9781 gatgcatgta ccatctcaga gcgttatggt gaagattttt ttgatatggt tgtttcaata
     9841 gagacaatag aacacatcaa tactcctgaa gtttttctta gtgctatcaa aaaaactgct
     9901 aaagaaaatg cgattttcta tataacttgc cctaatgatt attggtatta tccaaatgat
     9961 gaacaatcca atccgtacca tgtacgaaaa tatacttttc aagagttcac cgaacttagt
    10021 acagtgattt tgggaaagaa tgttcaatgg gcatatggtt gttctgcttt tggattttgt
    10081 acagtctcag caaataatcg aggttttgaa aaaataggtt cttcatggat ggaaataagc
    10141 gattcggaaa attcaattac tgttttaaat acagatattg acgcagttag tgaaaataat
    10201 tgcagttttt ttgtgggctt atggaatgca cctgaaacaa attttagcaa tagtacattc
    10261 ccaataagta tgaatgctta ttcgcgcatg acagaagagt tcgagtcgaa tgtaagtctg
    10321 gtacttcgtg aggaacaggc agaaaataaa aaagctatat caatattaaa acaagatatg
    10381 aaaaagttaa caatggacct gagaaaatca aaccttttat tattcgcatt aaaaatcgaa
    10441 aatgatgcat tgcgtactaa tatttccgaa ttgcaacaac agcttgccag gaaaattgcg
    10501 atgtatgatg aagtaacaaa tacaaataat aatttagtaa ctacaaattc atcattgatg
    10561 gaaagagtgt cacagatgga aattccatat tatcgatacg taaggctatc cgcccttctt
    10621 ccggattttt tgaaaaaaat gatacttaaa attatgcgtt tgatcaggaa ataaaaatgt
    10681 cggcacgttt aaaattatta ccaggggtaa aatcaactga atatgtgtat tcagaccttc
    10741 atgctttgct cgattctaat gctgaaagct cgtttggtcc gaacattggt agtgatggtt
    10801 ctaacctaac aataacatgt ttatcgatga gtagagtttt tcttactgaa aaacttgtta
    10861 attctatata tcagcatata cctaatttca aaggtgatat cctgattgtt gataacggca
    10921 gtacattaga agaactttca gttttacaaa ctttaagtga taggattcca ttacatatta
    10981 gggttatcga acttggtaat aattttggcg taagtggcgg aagaaataaa gcgttagagc
    11041 atataaaaac agaatgggct atgtttcttg ataatgatat ttattttata aacaacccgt
    11101 tcccgagatt gcaaaaggat atttcaaaac ttggctgtca tttcattaat atgccattgc
    11161 ttgattctga tggagaaaca ttattcgcaa atggagggaa tattttcctc catattgata
    11221 atgattcggt gcatgttggt gcaggaagtg catgcgtcca ggaaaaaata actacatatg
    11281 atggtgaggg cttcctttct acatttctct ttggcggtgc gagtgttatt aaaattgact
    11341 ctctgaaaag tttagggaag tacgatgaaa atatgttcat tggatttgag gatattgatt
    11401 tatcgcttcg tctttaccag tcaggaatga aagtaggtac atgtggtgca atatctttaa
    11461 tccatgatca ccccaaacct agttctaata aagataagga ttatgagaaa attcgcttca
    11521 cgaaaagtat attaaaagag tctgcggatt atcttgaaaa caaatgggga atggttttct
    11581 ggtcagctcc agttgatgat tggttggaag agaagcaacg tagttttgaa ctgataactc
    11641 aaaaagaaag taactattcg caaattgatg ataataaaca aaatgtcatt tgtgattttc
    11701 cagtagcaaa accgagaatt gcattgatta ttgatactga aaactgggca ttctcaaata
    11761 tagcaaatca aatagtcaat catctgtcag attcgtatga ttttacaata ataccaactg
    11821 aaatcattga taatatcagt caggttatta tgatgaccaa aaatcatgat ataactcatt
    11881 tcttctggcg tgaatcgctt agacttatat atgatgaatg ctatataaac tataaccggg
    11941 ctattggatt tgatgagctt agttttaaaa aacaatactt agatggaagg ataataacaa
    12001 gtagtgttta tgatcatcta tttcttgatg aacaagctat aaagttaaga aaaacatttt
    12061 ataatgattt aataactgct tatacagtta gttcatcaaa attatatgat atatactcat
    12121 caatagccga gtatcctaat ccgtcagttc ttgctgaaga tggtgtcgat ttaaaactat
    12181 tttatcctct cgatcttgaa cgctttgaca atattgagtc gagaacattg cgcgtaggtt
    12241 gggctggtaa ttctaaatgg gcgggagaag tagaggattt taaaggttac cattctttat
    12301 taaagccagc agttgagcaa ttacaatcag aaggattgaa tatagagtta gtccttgctg
    12361 atcgtcagct cggatttatt cctcatgatg aaatggtaaa ttattatagc caaattgatg
    12421 tctatgtctg tccttcaaaa attgagggca caccaaaccc tgtactcgaa tctatggcat
    12481 gtggcgttcc cgtgataagt actgatgtcg gtgtggttaa tgatgcgttt ggtgatatgc
    12541 aaaaagaatg gattttgcca gttagaagca aggatgtttt gatagataaa cttcgggatt
    12601 tttataatag gcgtaacaca gtggtaaaat gtttatcttc agaaaatctg cagcaaataa
    12661 aaaaatggga ttggaaagtt aaatctgaga acttccgcat ttttttcgac aaaatattag
    12721 aatctaaaaa gcacagcaag ataaacactg aaatgtgatc aaaatatcat tttaacctta
    12781 acacaggttt tttatcatta tcgtgagtta ataagatcgt agtatctaca cctgacagga
    12841 gtatgtaatg tctaagcaac agatcggcgt catcggtatg gcagtaatgg ggcgcaactt
    12901 ggcgctcaat attgaaagcc gtggttatac cgtttccgta ttcaaccgct cccgtgaaaa
    12961 gaccgaagaa gtcattgctg agaatccgga taagaaactg gttccttatt atacggtaaa
    13021 ggagtttgtt gaatcacttg aaactcctcg tcgtatcctg ttaatggtga aggcgggcgc
    13081 gggtacagat gctgtcattg attcacttaa gccgtatctg gaaaaaggtg acatcatcat
    13141 tgatggcggt aatactttct tccaagatac tattcgccgt aaccgggaac tatctgccga
    13201 aggttttaat ttcatcggca ccggtgtgtc cggtggtgaa gaaggggcgt tgaaaggccc
    13261 atctattatg cctggaggtc agaaagaagc gtacgagtta gttgctccaa tattgactaa
    13321 aatcgcggcc attgctgaag atggcgaacc gtgcgtaact tatatcggcg ctgatggtgc
    13381 aggccattac gtgaaaatgg tccacaacgg cattgagtac ggcgatatgc agttgattgc
    13441 tgaagcctat tctctgctaa aaggtggtct taacctgtca aacgaagagc tggcagacac
    13501 ttttaccaag tggaatgaag gtgagctcag cagttacctg atcgacatta ccaaagacat
    13561 tttcacccaa aaagatgaag agggtaaatt cctgatcgat gtgatcttgg atgaagcggc
    13621 taacaaaggc actggtaaat ggaccagtca gagttctctg gacttgggcg aaccgctatc
    13681 tctgattacc gaatctgtat tcgctcggta tatctcctcc ctgaaagacc aacgcgtagc
    13741 agcttctaag gtcttgaccg gacctaaagc tcagcttgca ggtgacaaag tagagtttat
    13801 tgagaaagta cgtcgtgcat tatatctggg gaaaattgtg tcttatgcac aaggattctc
    13861 acaattgcgt gctgcatctg atgaatataa gtgggatctg caatacggtg agatagcgaa
    13921 gattttccgt gctggctgta tcattcgtgc acagttcttg cagaaaatca ctgatgctta
    13981 tgctaacaat gataaaatcg agaacctgct cctggatccc tacttcaaga atattgctga
    14041 tgaataccag caagctctgc gtgatgtggt aacctatgcg gtacaaaatg gtattcctgt
    14101 accgacgttt ggcgcagctg tcgcttatta tgacagctac cgtgcagcgg tactaccggc
    14161 gaacctaata caggcgcagc gcgattattt tggtgcacat acctataagc gtactgataa
    14221 agaaggcgta ttccatacgg aatggttgaa ttgaataata aattttcata ttgtctattt
    14281 taataacaag gccatcttac agggtggcct tattgtcagc aaaatttctt gctgtattaa
    14341 atatttatat tattctatat ataaatattc ctcattaaag gcattggttc gaacatgcga
    14401 aaaaatataa taattgtagg tggtggtttt tttggaatgt acctcgctga gcaattttct
    14461 cttttaggtc atggagtaaa gctattcgaa aaggagaata attatatgtc tcgagcgtca
    14521 tataacaacc aggcgcgtgt gcacaatggc taccattatc caagaagtat tttgacggct
    14581 ttacgttcac gtatctcttt cccgcgtttt gttgatgaat ttcgtgattg cgttgattca
    14641 gactttgata agtattattt aattagcgaa atactgggga aagtttcggc cagccaattc
    14701 cgcatgttct gtgaccgaat tggtgcagac tgtgattatg ctccagcgaa aatacagaac
    14761 atggttaatc caaaaatgat taatgcatgt tttacggtta aagagtttgc ctttgatgcc
    14821 tttaagttgc gcgatataat gctaaagaga ctggacagtt caaatgccga attctattgt
    14881 ggctattcgg ttgataaaat cgagcaactg gaaaatggta ttaagacatt gatcagagag
    14941 gtaagcacag gtgaagtgca ggagtatgtt gctgaccacg tgtttaattg tacttattca
    15001 catctgaact atcttctgga taactctggt attgaactga tccctctgaa gcatgaaatg
    15061 accgaaatgt gtctggtaga cgtgcctgaa gtatgtaaga atgcaggttt taccataatg
    15121 tgtggtccat ttttctccgt gatgccgttc ccttcagttg gattacattc atttagtcat
    15181 gtgcgttata cgccgcatta tgagtggtac gataaacctg gtgaaaaata tctcgattca
    15241 catgataaat ttgattcaga tacaaaaaat agcgcttggc aatacatgat tagagatgca
    15301 tcacgttata taccagtttt aaacgaatgc cgttatgagc gttccctatg ggaagtgaag
    15361 acccttcttc cacgcagtga aacagatgac tcccgcccaa tcctctttag gcctaatcat
    15421 tgtatacagg gattccactg tattatgggt ggtaaaatag ataacgtata tgatgctttt
    15481 gaagccataa aaagtatgaa cgttatttaa aatactatag agactaaaca agtgaaaagt
    15541 gaatcttttg tttcggttgt tttaagtacg cacgatttat cgagtgaatt aacaaataaa
    15601 ttaaagcaga ttcagtcttt actggacgat aattacactg attatgaaat tttaattgtt
    15661 tctaaaggac cgtatcgtga tgtaccgaga acatctgcga tcgacaatat attaaaaacc
    15721 attccgagcg tgcgctttct gcaactttcg ggctgcgttc atgatgatgt ggtgcgggct
    15781 gctggtcttg agaatgcaat tggtgatttt gtggttttat atgatcatca gactgatcct
    15841 gtttatgtca ttcatcaagc agtggaagag tgtaagtccg ggtacgatgt tgttgtgggg
    15901 gtctcaacgc agtcgcttcc gtgtgcgtat cgtattatgc gggatacggc gtcgcatata
    15961 ttaaaagcga tcgattacca tattcctaaa aactctacag gtttgcgctg tctcagtcgt
    16021 cgtgcggtaa atgcagtaac agatactggg cgtttccacc accagttcta tatgcgcatt
    16081 caaaaaacgg gctatcccgt cagcgaactg acttatcagc ccgttagcga aacgcatatt
    16141 aaacgttcta tcacatatgg ttttcgcaat ttagttcgtt tgatggtctt caactcttct
    16201 cgtcctttgc gctggatgtc gattgttggc actgtaggta gtttgagcgc tctgcttttc
    16261 gcggtataca gtattctcgt gaacctgatt aatggccatg ttgtagaagg ttggactact
    16321 acagtcttgt ttatgtccac tttgtttatg ctgcaattta ttatgttggc cttctttggg
    16381 gaatatctca gtcgtttgct cgatgaccga agcgaacagg cagcatattc cgttgtgttt
    16441 gagaaaaaca gtgcagtaat gattaacaaa gaccgcgtta atgtgttgaa tgatgcgact
    16501 gtggaagagg ctaatctggt gcaaaccgga cgtgaccgtt aaatacagga gagatcatga
    16561 ctaaagcttt gataggatat accgggtttg ttggcacaac gctcttaagg caaaccaaat
    16621 ttgatgattt ttatcgttct acaaatatta atgatattaa gggtaaaaaa tttgatctgg
    16681 tggtttgtgc cggtgcgcca gcgaaaaaat ggatcgcgaa tgccgatccc aaaggcgatc
    16741 aggaaaccat tgattcgctg attgaatgct taaataacat tgagtgtaaa acgttgatcc
    16801 tcatcagcac tgtggatgtg tttaaatcgc ccaatggtgt tgatgagaat tccgtgatcg
    16861 atacgcaggg gctgcatgca tatggtttga atcgttatcg ccttgagcgg tttgtcgcga
    16921 agcgattcca acaacatctg atcgtcaggc ttccaggttt ggtcggtcca gggctacgta
    16981 agaatattat ttttgatttt cttaatgaca ataatgtaga gaaaatcgaa agccgcagta
    17041 ttttccagtt ttatccgatg gttaacctct ggttcgatat taaagtcgca cttgagaaca
    17101 aaattaatct tattcatctg acagcagccc cagtttcggt ggcggaaatt gctctcgatg
    17161 gtttcggtat taattttgat atgcagcagg cggcacaacc tgttttttac gatatgcgta
    17221 gtgtgtatgg tgcattgttt ggcggcagcg cgcattacca atattcggta aaagatacgt
    17281 tgcaggctgt caggcattat gcgcagtcag aacccaaaaa aagagcagga atacaataaa
    17341 tgagggtatc aatctccaac cttgcctggg aagttaccga agatgtagaa gttgctaatc
    17401 tattgtctgc atttaacatt gacgcgattg atatcgctcc cggaaagtat tttccggagc
    17461 ctactaacgc gactatagca gatattcaaa cggttaaaga gtggtggcag gagagaggca
    17521 ttgacatcat tggcatgcag gcacttttat ttggaactac aggtctgaat gtatttggta
    17581 gtaaatctgt tcagaaaaaa ctactcaatc acttggttgc agtttgccgt ataggtgcag
    17641 ggctgggggc tacgcgcttg gtatttggtt cgcccaaaaa tcgggactgc tccggtctat
    17701 cagttagtca ggttcagaac atatcacaga aatttttccg tcagttgggc gatatcgccg
    17761 ctgaccatgg tgttattgtt tgccttgagc caaacccaag tgtctatggt gcaaacttta
    17821 tgacaaccag ctttgaaacc tgtcgtgtgg ttcatgagat tgatcatcca gctatcaaaa
    17881 tgcagcttga taccggagcg gttattatca atcaggaaga tgtttctgat gttgtgacac
    17941 aagcaaaaga tgtgattggg catgtgcaca tcagtgaagc gcagcttgcg ccgccagggg
    18001 actcatattt tcgacatgat gatattgcag cggtaatttt atcgcaatta cctgcgcata
    18061 cggctactat tgaaatgctg gcaaccagaa atgagccgca tttagtttct attcgtcgcg
    18121 cgcttcaggt cgtgacttct ctttatcgtt aagcaggtgc atttaacttg aaatggttaa
    18181 ttcttattct gggcattgca tgtaatgcct cggctagcgt tctgatcaaa atggcaatga
    18241 tgcctccgcg taaatttcct tccctgggag atccattagc gtcgttgagt aactggcctt
    18301 tctggctggg cttgactctt tatggtggtg cgttcttatt ctatgcgggt gcgctagccg
    18361 tttttcctct gaacattgca catcctgttt tgacaactgg tgcggttgca gcagtggcag
    18421 ttttatccgt cttggttttc aatgaaacct ttcaatggac gacaattgct ggcgtggtgt
    18481 ttgtcatcgt cggtgttgca ttaattacgt caaaagctgc ttaataatgg atttatcgat
    18541 gaaacatgaa aaaaattggg aagtaccgtc gtacgatgtt accttgtgga cggaaaaaaa
    18601 acatgacgca tgtgtcgtga tcccggtgat taatgaagga agccgaatta ttagtttact
    18661 cacgaaaatg agtaatctta aaattaataa tattgctgat attattatcg tcgatggcgg
    18721 cagcactgac ggttctcttg aaaaagatcg cttgttatcg ctcggagttt ctggattggt
    18781 acttaaaatc ggtccaggaa agctgagtgc gcaattacgt tgtggttatg cgtttgtact
    18841 tgaacagggt tataaaaata tcattactat tgacggtaat gataaggatg atccgtctcc
    18901 aatccctgaa ttccttaacg cactggaaaa aggctatgat tttgttcagg cttcgcgctt
    18961 tatcaatggt ggtgttgcgg taaatacacc aaaactgcgt gatttcgcga tcaaatatat
    19021 tcacgctcca gccctcagca tagcatctgg ttttcactgg accgatacca cacaaggttt
    19081 tcgtgcctat agcagcaaat tgctgctaga tggccgtgtg gctcctttcc gtaatatctt
    19141 tatgacctac gaactattag cctatctttc ttatattgca ccaaaattag gttttcgctg
    19201 cattgagctt ccaacttctc gtatataccc aaatggtgat gtgccaacta aaattagcgg
    19261 ctttaacgga aatctgagtg ttttgataat actttttaaa acatgttttg gacgatacaa
    19321 ttctaatcgg tgtaactaat taaaactatt tttttttgat gcgaattttg gtttttacaa
    19381 tgtatgaata attgggtgtc gttatgaata tttttggctt tgaccacgag cgtgtaaaaa
    19441 ttgaagttgt tcttctgatt atatgtgtgg gaggaatgct gtgttcaatt tatgcatttt
    19501 atcctgggtg gatgagtccg gattctatct ttcaatataa caatgcggtt acaggatatt
    19561 ataattcatg gcatcctgtc attatggctt ggtggtggag agttcttggt tatataacag
    19621 atggtccggg gcctttttta gtccaaaatt taatgctgtt ctggattgga ttctatttta
    19681 tagctaagac cttatcaagg tacatcggtt ggggtagtat agttgtatta ctattggctg
    19741 caacaccttc agttttaata attatggcgc agctatggaa ggatgcagta tttacttcac
    19801 tggcaatatt tggcttaggc atcatctttc gctctataga tacagggaga ttttataaga
    19861 gtgttttttt gctttttgtt tttgtgctct ctcttgctgt gggatgtaaa ccaaatggtc
    19921 tgtttgttgc cattatattg atagggtggt ggtttattgc taatcctaaa gtgagtacta
    19981 aaaggtctaa gttaatactt tcattgacct ctgtattttt agttatggca ataccatttt
    20041 ctatttctaa atgtcttcct gtttataaaa tatcaccgat acaatatata cagtcatatg
    20101 atttacttgg tataagtgta atagaaaata aaacccttct tcctgattat attactaaaa
    20161 aagttgggtt gagtaaggat aatgcccggg agttttactt tccagggggg aataatttga
    20221 tgttttacaa ctcaccagct ggtaacatag cgacgacaga tcctgaatat ttaaaggatt
    20281 tacaatccag atggatatcc gcaataaaag actatcctat ggcatatatt aaggttcgat
    20341 ttgataattt tatagaatta cttcgttggg gcgctattca tcccgcttct gttgcggctg
    20401 atgttatcgt aaataatcca tgggggtatg tttttattcc caacaaatta agtgataatt
    20461 atattgattc aatagataaa tacccttttt tgtatttccc atggatttat ttggttacat
    20521 ctgctttatt attcttgtta acaaaggttt taccaagaga gttgcgattt tttaatgttg
    20581 ttcttgtact tgttgtcttt tcgttttcgc ttcctcattt ttttattgct ccagctgctg
    20641 attatcgtta tctgcatttt tcagttgttt gtgcagtggt tcaattagga atgtttttgg
    20701 gctttttctt tatgagattg tataaaagag tgtattttgg ggcgattaaa ttgttgggtt
    20761 aagggtgttc ttctgttatc acatatttta tctatatatt tataggttta ttagattggg
    20821 taattaagtg ttgtctgtat cttggtgctc ttgttgaata tgttttttag cccagctcta
    20881 attgtctgtg cttttttgcc taaattatta ttgattttta ctttttcctt aatattaact
    20941 taataaatga ggtcatatag taatgaagta aatctgaatt gtgccgtcgg tcattgacat
    21001 ttcatcaact cagacgttac aacttttaat acatgtcatg atgatgcaaa catgatgagc
    21061 aaaagttaac cctgagaaaa ttaaatgaaa atcactattt ctggtaccgg atatgttggc
    21121 ctgtcaaatg gtattctgat tgctcagagc cacgaagtgg tggcactgga tatcatccag
    21181 acgaaaatcg agatgctgaa tcagaaacaa tccccgattg tggataaaga gatcgaagag
    21241 tatttggcga ctaggtcgct aaacttccgt gcaacgactg ataagtgtga tgcctactta
    21301 gatgcggact acgttattat cgcgacacca actgattatg atccaaagac taattacttt
    21361 aatacctcaa gtgtcgagtc tgttattcgc gacgtggttg caattaatcc agatgcagta
    21421 atggtaatta agtctaccat cccagtaggt tttactaatt ctatcaaaga aaaactgggc
    21481 accgaaaata ttttcttttc cccagaattc ctgcgtgagg gtaaagcact atatgacaac
    21541 ctttacccat ctcgtattgt cattggtgag cgttcagagc gcgccgagcg ttttgcagct
    21601 ctgctacagg aaggggcact aaagaaagat attccgaccc tatttaccga ctcgaccgaa
    21661 gccgaagcta ttaagctgtt cgctaatacc tatctggcgc tgcgtatagc ctactttaat
    21721 gagctggaca gctacgctga aactctaggc ctgaattcac tacagataat tgaaggggta
    21781 tgcttggatc cgcgaatagg aaaccactat aacaacccgt catttggcta cggcggttac
    21841 tgtctgccta aggataccaa gcagttgctg tccaactatg aatcggtacc gaataactta
    21901 atcggtgcga tcgtcgaagc taatcgcact cgtaaagact ttattgccga ctctattctc
    21961 gcgcgcaagc ctaaggtggt gggtgtttat cgcctgatta tgaagtcggg ttcggataac
    22021 ttccgcgcgt cctccattca ggggatcatg aagcgtatta aggccaaggg tatcacggta
    22081 attatttatg aaccggtgat gcaggaagat gagtttttta actcccgcgt ggtacgtgac
    22141 ttcgaagcct ttaagctgga ggcagacgtg attatctcca accgtatggc agaagagctg
    22201 gtggatgttg gagataaagt ctatacccgt gatctgtttg gtaatgatta aacgttagtt
    22261 tttgtgtcgt tctcggcggt gcttcgcttg gccgggctat gttagaaaaa gccctcatct
    22321 tggactgcac cccaaaagtt ggacacccaa ctgattaagg tgcagttttt tatggcaaag
    22381 ccaaaatatt ctctcgaaac cagattagct gtcgttactc attatcttgc cggacacgat
    22441 ggcgcacgtc gtaccgctga acgttttggt gttgaagaaa catcagtgcg acgcagggta
    22501 agagcctggc aactccacgg tattgatggt attacatgga aaaatgaccg ccattctcct
    22561 gagtttcgac tgacggttgt cctgaagtct ctgagtgaag aacttaccat gcgcgaagcc
    22621 gccgcacggt ttaatatctc caatgaatcc gttgtccggc actgggtgaa ggtttacaaa
    22681 gacactagtg aagcaggact tctgaacata aaaccaggcc ggagcaagga catgacaaaa
    22741 cctctaaaaa cacctccact tacgatgaag aattggaaaa gttatctccc gaagaactgc
    22801 gggcggaact ccgttacctt cgcgcagaga atgcctatct aaaaaagctg aaggccttag
    22861 ttcaaagcga gaaaaacggc agaaagcccg gataatcagt gagctaaggc tgaagcatgc
    22921 gctcggtgat cttctgcgtg ccgcgggtat gtcccgcagt acgtggtatc acaatatgaa
    22981 tgccctgaag cgagtggaca ggcatgccgg actgaaagac aaatcagaga gatttatcat
    23041 tatcacaaag ggcattatgg ctaccgcaga atcacgctct cactgagaaa atagggcgtg
    23101 ttggtaaacc ataaaacagt gcagcggctg atggcagagc tgtcgctccg ctctgtgata
    23161 agggtgaaga aatatcgttc ctggaaaggg gaagtaggca aagctgcacc caatatcctg
    23221 atccggaact ttggtgcaac gaagtccaac gaaaaatggg tgacggatgt tacagagttc
    23281 tcggtacagg gtaaaaagct gtacctgtcg acagttctcg atctgtttaa ccgggagatt
    23341 atctcctaca gcctgtcaga aaggctggtg atggagatga ttaatacgat gctgcacgat
    23401 gcattctcag tgtttggacc ggaggatgca ccgttgctgc acacggttca gggctggcaa
    23461 tatcgaatgg caggctatca ggcaacgtta aaggcccatg gaatgacaca aagtatgtcg
    23521 cgcaagggaa actgtctgga taacgcagtg atggagaact tcttcggcgc actgaaatcg
    23581 gagtgttttt acctgaacag gtttagcgat ctgaatgaac tgaggaaagc gatagaggac
    23641 tatatccatt attataacaa cgagcggatc agcctgaaat taaaaggcct gagtccggta
    23701 gaataccgaa ctcaggccct gaaagccgct taacatgaac tgtccaactt tatgggggca
    23761 gtccatccat gagggctttt ttttgtgcac agaaaatccc cagctaggct gagggggggg
    23821 ccggaaagct ttcagctttg agccagttat taaaaccctt ttgatttgtt aaaacacctt
    23881 gcggtctggc aactgcaagt gtcaaacaag aaatcaaaag ggggggctaa tggggaacaa
    23941 agagagctta gcgcacaccc gattgaacga taaatctcac atattttttg cgccaaaata
    24001 ccgaagacag gtggtaaggt aatccccccg aaaaaaccgg tgcgatgtcc gggttaggaa
    24061 agaggattac tgatgtcttt tgcgtaaatt ctcaatcacc gtcgtcaggt ctaggttctg
    24121 atcctgcaac agcaccagca ggtgatacat caagtccgat gcctcgttgg tcagttcgaa
    24181 gcggtcatgt actgtggcgg ccagtgctgt ttccacgcct tcttcgccca ctttctgcgc
    24241 aatgcgtttg gtgccgctgg catacagttt ggcggtatag gaggtttccg gatcggcaga
    24301 tttacgttcg gcgagcagtt gttccagttg atacaggaac agccattggt gagcggtgtc
    24361 gccgaagcag ctgctggtgc ctttgtggca ggtcgggccg atgggattcg ccagcaacag
    24421 taacgtgtcg ttgtcgcagt ccggcgtaat atttactacg ttgaggaaat tgcccgacgt
    24481 ttcgcctttg gtccacagtc gctgtttagt gcgcgagaag aaggtgactt tgccgctttc
    24541 gatggtttta tctaaggctt ccgggttcat atagcccagc attaacactt caccggatac
    24601 cgcgtgttgc acaatcaccg gcataagtcc gtcggttttt tcccagtcca gttcgcggcg
    24661 ttgttgttct gttaacatat cctgatctcc acgccctgtg ttgccaggta cgcttttaat
    24721 tcaccaatat tgattatttg tttgtggaat acggaagctg ccagcgcacc gtcaacgtcg
    24781 gcatcgcgga aagcttcgag gaagtgttcc atggtgcccg cgccaccgga ggcaattagc
    24841 gggacgtggc aaacttcacg tacttttttc agttgttcga ggtcgtaacc gttacgcacg
    24901 ccgtcctgat tcatcatgtt gaggacgatt tctccggcac cgcgtttttg cacttcctgt
    24961 acccagtcga gcgtttccca ctgagtgacg cgggtgcggc t
//
