LOCUS       EU296420               14006 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O164 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296420
VERSION     EU296420.1  GI:187880637
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O164"
     gene            2557..4011
                     /gene="wzx"
     CDS             2557..4011
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37142.1"
                     /db_xref="GI:187880638"
                     /translation="MFLATRTLVSLSVSFYTTRIVIQQLGAADYGLFNIIYGVVTFFT
                     FVVTAMNDSVQRYIAIGVGSQKISVIRDAVKNSMFIFVISAFILAVCLLLARGVIIHN
                     ILNIPKDSIENASVLYLVAVFSITILIIQTPLNAMVLAYEKMSFYAYMMIFEMVAKMS
                     MALLLTLLEKDKVIVYSILLGSISFFNLLVYLCYCLICFKKSMFGGRIKFRVLKEIST
                     FSFWNIFGNFSYMCRVQGVNIVINMFYAIAVNAAYAISITVLNAINTLTQSLITALRP
                     QIFKSYGECDLKRYNHLVLFGSKYTFSILFLLSSPVILCADELLKIWLDIVPDYTVEF
                     VRLVIVVAFIDSFSYSMIAGIQATGRIKTYQLVVSLIVLINLPLTFILFKAGNNVLSM
                     FYPFIVTAIINQGLRLYFIYINAGFDYKKYFTVVIYPCLLAVCLSLVTDISIKKMLPF
                     NSVIDVLIVCIFIFSFNTIIFYWVVVSKKEKNGY"
     misc_feature    2557..12618
                     /note="O-antigen gene cluster"
     gene            4001..5383
                     /gene="wfeO"
     CDS             4001..5383
                     /gene="wfeO"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeO"
                     /protein_id="ACD37143.1"
                     /db_xref="GI:187880639"
                     /translation="MVIRNPEKKSEITIKDVIDNELCTGCGVCISEDSSKTSFMKWNS
                     EGFYEPCFSPVSTLFNMQRVCPFNLSRDTLVNEDELAHEFFDGKGYLDSEVGFYKKIY
                     VGYSKHFRETSSSGGIATYVFEQLLRRKYVDALFIVRELGGSYGYQVFDNPEHIKDMS
                     KTRYYPVTLEKLFDNIYKLNGRVAVSGVACFIKAIRLKQHYHPELKEKIPFLVGIICG
                     GLKSRYYTDYLSQSAGCVSEYQNAEYRVKKKDSHALDYRFTCVEKSNNIIHSVDMQRM
                     GDMWGSGLFKANACDYCDDVTTELADISLGDAWISPYNMDGAGNNVVVCRSTTAHEII
                     LSGIEKKDLELTELELEQLKLSQQGSFNHRHKGLLYRIKNAEKNNRLVPVKRKRFLRS
                     ISFLLKLIQKQRSVTRRKSIEIWMETQNSATFDKKMKGYLFTLRWLTVVNRKLSRMFK
                     IVSLNRMGKK"
     gene            6561..7565
                     /gene="wfeQ"
     CDS             6561..7565
                     /gene="wfeQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeQ"
                     /protein_id="ACD37144.1"
                     /db_xref="GI:187880640"
                     /translation="MKNCKVSVIIPVYNAEKYIQRCILSLLKQTLDDVEIIIIDDGST
                     DNSLSIIKETVALHTASRARQKRINIISRENKGVAYTRSQGLRLSQGEFIIHFDSDDW
                     AKSNMLEEMYKTIVSNNADMVICDYFLVKNNKEILIKQRVEYEPKKCIRYLLTGELEG
                     FTWNKLIRKKYIDKNKIDFVNKITYMEDFLFILSVLLHNPKIIFQDCAYYYYQKSNPK
                     SLTSHASVDRLSEMIKAVSEIEKKINKYNLQKYLNNEFQLFKLKQKIWFISISKLNVN
                     DNVWNLFPETNPFISKVNVLFYYKIVLFLDSIKLRFFSNKIIYLIGLVQALLQERKEK
                     "
     gene            7562..8350
                     /gene="wfeR"
     CDS             7562..8350
                     /gene="wfeR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeR"
                     /protein_id="ACD37145.1"
                     /db_xref="GI:187880641"
                     /translation="MIPKIIHYCWFGRSPLSELTKQCIASWEKYCPEYKIIRWDENNV
                     DLNSCSFVRQAYKEKKWAFVSDYVRLKVVNEYGGIYLDTDVELIKPLDDLLIYPAYIG
                     FEINKEWYVNSGLGFGSVNNNPVLESLIMEYENINFVNEDGTLNITPCPIRETKALTK
                     IGLIPDGQCQSFDNIVIFSADYFCPVSITGERNFSDKTYSIHHYDASWFSEQKRKGLQ
                     RKKRFIKLFGNLIGTYINKPFIFVDECREFGLVKAIKNMRSNFP"
     gene            8424..9527
                     /gene="wzy"
     CDS             8424..9527
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37146.1"
                     /db_xref="GI:187880642"
                     /translation="MYFLLFFSIFYFVYVILIGTLCHNYKALLIFSLIPLTIVSGIRY
                     NVGFDFMSYVDYFNQLKYSNDIYLDSTFKYISYFTYYIGGNEQILFLIYAVFYSVALY
                     FLIKLVLENYITEINNFYTIGLVLSFYSFYFLLSFNQIRAVLSALILCYGLLKNKIDF
                     SFIITITLSILFHSAAMFILPLYFVLRKINVTVLLVIFPFLVVASFFNIFSDIVRFIL
                     TLLNSRFLTYFNSEYFVPRTGMEKMYSMISMIIVLGMVVCLSKLLPKKFDLMIKFVIL
                     FVLLRAMSIDILIFARFSDFLKPMAIILIFTAVYFSSNKVRPRIILPFYLIMTLFLCI
                     FNIIIGSNITKGNYYTYGYNICFFGNKCIDKFY"
     gene            9556..10605
                     /gene="wfeS"
     CDS             9556..10605
                     /gene="wfeS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeS"
                     /protein_id="ACD37147.1"
                     /db_xref="GI:187880643"
                     /translation="MEKYMNNFSCKEPPSFIENKIILSIILPVYNVSEYLIECLNSLL
                     LDVHERYLNRCEVIVVDDGSTDNSFELMREYSLKYPESIKIYSKFNGGLSDARNYGLL
                     KSSGKYISFVDSDDVVNRGFIKEIINFIDAYDFDILSFDFMKFFNNNDALILSQIDEF
                     SKMAERVDSEFYKSKPVFAWNKVYRRSLFDNEVFPKGWYYEDVALIPLLLDRAKVLYH
                     INSVCYFYRQRQGAITFFYDNKYLDILKGVSFLYDRSQSSFIKTIIINQFFTLTLLSL
                     RLPTDNYFMNMRGIIDTYCEKFDLDSFEPKLILKHIPFLLLKKLKSLCVYPLYLFKPA
                     VFLHKQIKHFRGKLK"
     gene            10602..11711
                     /gene="glf"
     CDS             10602..11711
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37148.1"
                     /db_xref="GI:187880644"
                     /translation="MKYDYIIIGSGLFGSVFAHELGKMKKKVKVIERRDHVGGNVYCE
                     NIEGINVHKYGAHIFHTNDKEIWDYVNQFVEFNRYTNSPLAFSKGKIYNLPFNMNTFN
                     QLWGAITPQHAKEIIKKQSGEISGNKPRNLEEQAISLVGRDIYNCLIKEYTEKQWGRP
                     CTELPSFIIKRLPVRFTYDNNYFNDQYQGIPVGGYNKLIDALLNGIEVQTCTDYFEEK
                     KYWDSIAKKIIYTGPIDKFYCYKFGALDYRSLKFEHELLNVDNFQGNAVVNYIDKKFP
                     FTRIIEHKFFEFGEQHNTLITREYPKEFEEGDEYYYPVNDEKNMQIYKMYYNLSKQES
                     NVIFGGRLAEYKYYDMHQVIKSALNAFRKEVRLGE"
     gene            11704..12618
                     /gene="wfeT"
     CDS             11704..12618
                     /gene="wfeT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeT"
                     /protein_id="ACD37149.1"
                     /db_xref="GI:187880645"
                     /translation="MNKEVIAVIVTYNRKALLLKVIDAVINQSYPLKKILIIDNNSTD
                     GTEMFISNRLSDVVKYKNTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDFMPTVNCL
                     EILISNSPDGIVQPVRYNLDETCAELSPLTYDLSNPFKLNPKGTPLKNYLNTIGNKSA
                     KVDIEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRKGIPIQCNLKAKAY
                     RLLVNNQGNDLLSWKGYFMLRNLFYLHKTYGTNFLVRQKPIVLGLGYALSCVLKCNFS
                     QLPVIWRAFWDSSTLRNTEKFRPEMRSK"
BASE COUNT     4487 a   2161 c   2821 g   4537 t
ORIGIN
        1 ctcctggtaa ctcacgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgaa
       61 ttagaatctc tccttgagca gcgcgtgaag cgtcaactgt tggcggaagt acaatctatc
      121 tgtccgccgg gcgtgaccat tatgaacgtg cgtcagggtg aacctttagg tttaggccac
      181 tccatcttat gtgcgcgacc tgccattggt gacaacccat ttatcgtggt actgccagac
      241 gtagtgatcg acgacgccag cgccgacccg ctgcgctaca accttgctgc catgattgcg
      301 cgcttcaacg aaacgggccg tagccaggtg ctggcaaaac gtatgccggg tgacctctct
      361 gaatactccg tcatccagac caaagaaccg ctggaccgtg aaggtaaagt cagccgcatt
      421 gttgaattta tcgaaaaacc ggatcagccg cagacgctgg actcagacat catggccgta
      481 ggtcgctatg tgctttctgc cgatatttgg ccggaactag aacgcactca gcctggtgca
      541 tgggggcgta ttcagctgac tgatgctatt gccgaactgg cgaaaaaaca gtccgttgat
      601 gccatgctga tgactggaga cagctacgac tgcggtaaaa aaatgggcta tatgcaggcg
      661 tttgtgaagt acggactgcg taacctgaaa gaaggggcga agttccgcaa aggtattgag
      721 aggttgttga atgattaaaa acatgaacaa atataacggt aaataagaga aattgtaacg
      781 gcagtgagga tttgcggcga agctcaaacg tggcgaatat ccctgtcgtt atttattaat
      841 aaatcatcgt actaacaatg gaataaaaaa gtgccttgtt ttaagtttta caggatattc
      901 cttgtttctg gaggagattg ataagacaat cagcatctga atttatcaga acttgtgtgt
      961 cttgctgatt acattgttat attcatgcag tgtactggta gctgtggagc caggggcggt
     1021 agcgtgcatt aatattcaca atatattctt ttaaatgtaa gcagaattaa tcgaacctaa
     1081 ataatgtcac gtaacaaaat aatcataaaa atgtaatgaa cgcatcccac acaattaaac
     1141 acggagcagt atgatggtta aaagggtgaa actggcaggt gtggagcctc gcagattccg
     1201 gcatcatagt gcccaatacg ggatgcataa agctccctca gactggagac tccacaaatg
     1261 aaatatacac cggttggcgt tgatatcgca aaacatgtca ttcagattca cttcatcaat
     1321 gagcacacag gtgaagtggt tgataaacag ttgcgtagac aggattttct gacgttcttc
     1381 ggcaaccgtg agccatgcct gattggtatg gaggcctgtg gaggttctca gcactgggca
     1441 cgggaactga caaaacttgg tcataaagtc cggttgttgc aggcccgctt cgttaaggca
     1501 ttcgtcatgg gcaataagaa tgatgtgatg gatgcccggg ctatctggat ggcggttcag
     1561 cagccgggta aagaaatcgc cgtaaaaaca gaagaacagc agtcggtact ggttctgcac
     1621 cgtacccgca tgcaactggt gaagttccgg accgcacaaa ttaatgccct gcacgggacg
     1681 ttactggagt ttggtgaaac catccacaaa ggccgggcag cgatggagcg ggagttcccc
     1741 gaagcactgg aacggatgaa agagagactg ccaccgtatc tcattatggt tctggaaaac
     1801 cagtacaacc gactgaatga gctggactca ctgatagagg atattgaaaa acagcttacc
     1861 agcgtggcga ggcagaatga aacctgtaag cggttgctgg atattcctgg cgttggacca
     1921 cttattgcga cggcagcggt ggccaccatg ggggaagcat cagcgtttaa atcggggcga
     1981 gagttcgccg catatgttgg tctggttcca aaacaaactg gctccggagg gaaagtacgt
     2041 ctgctgggga taagcaaacg tggtgacact tatctcagga cattatttat ccacggtgca
     2101 agagcggtgg cattagtagc taaagagcct ggcccgtgga taaccgaact gaaaaaacgt
     2161 cgtccagcca gtgtggcaat cgtcgccatg gcaaacaagc tggcacgaac agtatgggcg
     2221 ataaccgccc atgaccgtaa gtatgacagg aaccacgtca gtatcagacc atattaatcg
     2281 ctgataccat taaacaatga actcttaaca aaagggtgaa tgctgaaagg ttgctatggc
     2341 ggccagagtg atgacaaaga caggtaagac cgtgactcac taaacctgaa cagtattttg
     2401 ggcttgaagt ccgccgtgaa aataaggggt gagtcggcga attacatagg ggctcgcagc
     2461 gttacggctg caataaagcc ggatataaag ctgcaaccta cccgtcatgt caaaacaatg
     2521 gatgccttgc aaacgggatg cgttcatata aatactatgt ttttggcaac aagaacctta
     2581 gtttctttaa gtgtttcatt ttacacaaca agaatagtta tacaacagtt gggggcggca
     2641 gactatggct tatttaatat catttatgga gttgtaacat tttttacatt cgtagtcacc
     2701 gcgatgaatg attctgtaca gcgttatatt gcaataggtg ttggttcaca aaaaatatcg
     2761 gttattagag atgctgtaaa aaatagtatg tttatatttg ttatatctgc tttcatattg
     2821 gcagtatgtc ttcttttggc aaggggggtg atcatacata atattttaaa tatacctaaa
     2881 gattctattg aaaatgcaag tgtgttatat ttggttgcag tattttcaat aaccatactt
     2941 ataattcaga cgccacttaa tgcgatggtg ttagcatacg agaaaatgtc attttacgca
     3001 tacatgatga tatttgaaat ggtagctaaa atgagtatgg ctttgttact aactttactg
     3061 gaaaaagata aggtcattgt atattcaata cttttaggtt ctatttcttt ttttaacttg
     3121 ttggtttatt tatgctactg cttgatatgt tttaaaaaat caatgtttgg tgggagaata
     3181 aaatttagag tgctgaaaga aatttcaact ttttcatttt ggaatatatt tggtaatttc
     3241 tcttacatgt gcagagttca gggagtgaac attgtaatta atatgtttta cgctatagcc
     3301 gtgaatgcag catatgcgat ttcgattact gttttaaatg caattaatac acttacgcaa
     3361 tcattaatta cagcattaag gcctcaaata tttaaatcat atggcgaatg tgatttaaaa
     3421 agatataatc atctagttct ctttggttca aaatatacat tttcaatatt gtttctgtta
     3481 agtagccctg taattctatg tgcggatgag ttgttaaaga tatggcttga tattgtacca
     3541 gattatacag ttgaatttgt gaggttggtc attgttgtgg cttttattga tagtttttca
     3601 tatagcatga ttgctggtat tcaggctacg ggtaggatta aaacatatca actagttgtt
     3661 agtttaattg tcctaattaa tttgccgtta acctttattt tgtttaaggc aggaaataat
     3721 gttttaagta tgttttatcc atttattgtt actgccatta tcaatcaagg tttaaggttg
     3781 tattttatct atatcaatgc aggttttgat tataagaagt atttcactgt tgtgatctat
     3841 ccttgcttgt tagctgtatg cttatcgtta gtgacagata tttcaattaa aaaaatgctt
     3901 ccatttaaca gtgttattga tgttttaatt gtgtgtattt ttattttttc attcaataca
     3961 ataatttttt attgggttgt agtttctaaa aaggaaaaaa atggttatta gaaaccctga
     4021 aaagaaaagt gaaataacaa taaaagatgt tatagacaat gaactctgta ctggttgtgg
     4081 agtttgtatt tcggaagata gtagcaaaac ctcctttatg aaatggaatt ctgaaggttt
     4141 ttatgagcca tgtttctctc ctgtatcaac attattcaat atgcagcgtg tttgtccctt
     4201 taacttaagt agggatacat tagtgaatga ggatgaactt gcgcatgagt tttttgatgg
     4261 taagggatat ttagattctg aggttgggtt ttataaaaaa atatatgtgg gttattctaa
     4321 acattttaga gagacttcat cctccggtgg tatagctaca tatgtatttg aacaacttct
     4381 tagacgtaag tatgttgatg ctctatttat agttagggag cttggtggtt catatggata
     4441 tcaagtcttc gataatccag aacatattaa ggatatgtct aaaacaagat attatcctgt
     4501 aacgctagaa aagttatttg ataatattta taagcttaat ggtagagtgg ccgtttcagg
     4561 agtggcatgt tttattaaag ccattaggct caaacaacat taccatccag aactgaaaga
     4621 gaaaatacca ttccttgttg gtataatttg tggaggactc aaaagtcgat attacactga
     4681 ttatttatca cagtctgctg gttgtgtcag tgagtaccaa aatgctgagt atcgagtgaa
     4741 aaagaaagat agtcatgctc ttgactatcg tttcacatgt gttgagaaat caaataatat
     4801 aattcattct gttgatatgc aaagaatggg ggatatgtgg ggttcagggc tatttaaggc
     4861 aaacgcatgt gactattgtg atgatgttac aaccgaactc gcagacatat cattgggtga
     4921 tgcctggatt tcgccataca atatggatgg agctggtaat aatgtagttg tatgtcgctc
     4981 caccacagct catgaaatta ttttatctgg gattgagaaa aaagacttag aattaacgga
     5041 actcgaatta gagcagttga aattgtcaca acaaggaagt tttaaccaca ggcataaagg
     5101 attattatat agaataaaaa atgcagagaa aaataatcgc ttagtgcctg ttaagaggaa
     5161 aagattctta cgctctatat cttttttatt aaagttaatt caaaaacaac gatcagttac
     5221 aaggagaaaa agcatagaga tatggatgga aactcaaaat tcagcaactt tcgataagaa
     5281 aatgaaggga tatttattca cgttgcgttg gttaacagtg gtcaatagga aattaagtcg
     5341 gatgttcaag attgtttctc taaatagaat gggtaaaaaa tgaaagttgc aatattaact
     5401 caaccattac atacgaatta tggtggcaca ttacaagcct atgcattaca aaaagtcttg
     5461 attaatttgg gacatgaacc agagactatt aattataggt cgaaaattaa gaggccacta
     5521 tttatccgtg ttgtgttgtc aaaaattaaa aggatagtat tatgccgaaa aatcacattt
     5581 gattttacaa cacaagatag aattaatatt aggaagcatc atcaatcttt cattgataca
     5641 agattaaact attcagagga gattaacggt actgaggggt tgagagatta tattttaaaa
     5701 aataattacg gtgctgtaat aataggtagt gatcagacat ggagaccaat atattcgcct
     5761 agaatagatt cattttttct cgatttttta agtgatgtaa acgatataaa aaatggcata
     5821 tgcagcatct tttggtactg ataaatggga gttcacggag tcacaaacta atttatttaa
     5881 attgttactt tcaaaatttg actatgtttc agttagagag tcctcaggag tcaaattatg
     5941 tagtgaaaaa tttggtttga aagcagagtt ggttttagat ccgacattgc tattgacatt
     6001 cgaagattat actcatttat tagacaatga ctatattaat cttcataagg gaaaggtgtt
     6061 tagttatgta cttgatgaga attacgacaa aaaaaacttt atagacacag tagcaagtac
     6121 tctccgcaca acgtcatttt atacttaccc taaaaaaatc actaaagatg aatatgtgat
     6181 tagaaattat tccgagtatg aatatccgcc aatagaatat tggatttctt cgtttaaggc
     6241 cgctgaattt attgtaacag attcatttca tggaacagta ttttctatta tttttaacaa
     6301 acccttcatc gccattgcaa atgaagagag aggaaaggct agatttacgt cactattgga
     6361 aatgtttggt cttgaaagac gacttgtgag taatttagat gatgttgatt taaagcttgt
     6421 aaatgaaaaa attaactatg gccccataaa tgaaaaaatt gcttatttta gagaagagtg
     6481 ccttaaaaag ttgcagttaa tgctaataaa ataatcagat tatgtatgtc tctctacaat
     6541 taaaatatga atgttgatcg atgaagaatt gtaaagtatc tgtaattatt ccggtctata
     6601 atgcggaaaa atatatacaa cgttgtattt taagcttatt aaagcagaca ttagatgatg
     6661 ttgaaattat catcattgat gatggttcta ctgataattc actttctata ataaaagaaa
     6721 ctgttgcatt gcatactgct agtagagctc gccaaaagag aatcaatata atctctcgag
     6781 aaaacaaagg agttgcctat acacgaagtc agggattgag actttcgcag ggggaattta
     6841 tcattcattt tgatagtgat gattgggcaa aatccaatat gctggaggaa atgtataaga
     6901 caattgtttc caataatgca gacatggtta tatgcgatta ttttttagtg aagaataata
     6961 aagaaatact tataaaacag agagtggagt atgagccaaa gaaatgtatt agatatctac
     7021 tgactggtga gctagaaggg tttacatgga acaaactaat tagaaaaaaa tatatcgata
     7081 aaaataaaat tgattttgtt aataaaatta cttatatgga ggattttttg ttcatattaa
     7141 gtgtgttact acacaatcct aaaattattt ttcaggattg tgcatattat tattatcaaa
     7201 aaagcaatcc taaatctcta actagccatg ctagtgtgga taggctttct gaaatgatta
     7261 aagccgtaag tgaaatagaa aagaagataa ataaatataa tttacaaaag tatctgaata
     7321 acgagtttca attgtttaag ttaaaacaaa aaatttggtt tatatctata agtaagttaa
     7381 atgttaatga taatgtatgg aatctctttc ctgaaacaaa tccatttata tcaaaggtaa
     7441 atgtgctttt ttattataaa atagtgcttt ttcttgatag tattaaactg cgtttttttt
     7501 caaataagat catttatctc attggattgg tgcaggcttt attacaagaa aggaaagaga
     7561 agtgatacca aaaataattc attactgctg gttcggtcgc tctccattgt cggaattaac
     7621 aaagcagtgc attgcaagct gggagaaata ttgtcctgaa tataaaatca taagatggga
     7681 tgaaaataat gtcgatttga attcttgttc ttttgttaga caagcttata aagaaaaaaa
     7741 atgggcgttc gtttctgatt atgtaaggct taaagtggtt aatgaatatg gcggtatcta
     7801 tcttgataca gatgttgaat taataaagcc attagatgat ttgttaatat atcctgcata
     7861 tataggcttt gaaattaaca aggaatggta tgtaaattct ggtttggggt tcggttctgt
     7921 taataataat ccggttttag aatcattaat tatggagtat gaaaatatta attttgtaaa
     7981 tgaggatggg actctaaata taacgccgtg ccccataaga gaaacaaaag cattaacaaa
     8041 aataggttta attcctgatg gtcaatgtca atcttttgat aatatagtga ttttttctgc
     8101 agattatttt tgtcctgtat ctataacagg tgagagaaat ttttctgata aaacatattc
     8161 aatacatcat tacgatgcta gttggttttc tgagcaaaag agaaaaggcc tgcaacgtaa
     8221 aaagagattt ataaaactat tcggcaacct aataggcaca tatattaata aaccttttat
     8281 ttttgtagat gaatgtcgcg agttcggatt agttaaagca atcaagaata tgcgttccaa
     8341 ttttccttaa tttttagtgt taaggaataa gtttcgttaa aatactttat tcatttaatt
     8401 aataaaggtt taatggtgat tagatgtact ttcttttgtt cttctcaata ttttattttg
     8461 tatacgtaat cttaatagga acattatgtc ataactataa agcccttttg attttttcac
     8521 taatccctct gacaatagtg tcaggtataa gatataatgt cggctttgat tttatgtcat
     8581 atgttgatta tttcaatcaa ctcaaatatt caaatgatat atatctagat tctaccttta
     8641 agtatataag ctactttacc tattatatag gaggtaacga acagattttg tttctgatat
     8701 atgcggtttt ttattcggtt gcactttatt ttttaataaa actggtattg gaaaattata
     8761 taacggaaat aaataatttt tatactattg gacttgtgtt atcattttat tcgttttatt
     8821 ttttattatc atttaatcaa attagggctg tattatcagc tctaatacta tgttatgggc
     8881 tattaaaaaa taaaatagat ttttctttta taataacaat tacattaagt attctttttc
     8941 actctgcagc aatgttcatt ttacctcttt attttgtcct aagaaaaata aatgtaacag
     9001 ttctgttggt gatattccct tttttggttg ttgcttcatt ctttaatatt ttttcagata
     9061 ttgtgagatt cattctgaca ttgctaaata gtcgtttttt aacatatttt aactctgaat
     9121 attttgttcc aaggacggga atggaaaaaa tgtatagtat gatctcaatg attattgttt
     9181 tgggaatggt tgtttgtttg tctaaattat tacctaaaaa gtttgattta atgataaagt
     9241 ttgttattct ttttgttcta cttagagcga tgtcaattga tatattgatt tttgctaggt
     9301 ttagtgattt tcttaaacca atggctataa ttcttatatt taccgctgtt tatttttctt
     9361 cgaataaagt gcgacctaga attatccttc ctttttattt aattatgaca ttgtttctct
     9421 gtatttttaa tataataatc ggatcgaata taactaaggg gaactattat acttatggtt
     9481 ataacatttg cttttttggc aataaatgta tagataaatt ttattagtaa ttgtcatttg
     9541 atacttctgg tatttatgga aaaatatatg aataactttt cttgtaaaga acccccttca
     9601 tttattgaaa ataaaattat attatcaatt attcttcctg tatataacgt atcagagtat
     9661 ttaattgagt gcctaaacag tttgctgtta gacgttcatg aacgttatct taatagatgt
     9721 gaagtaattg tggttgatga tggttcaact gataattcat ttgaattaat gagagagtat
     9781 tctttgaaat atcctgaatc tattaagatt tattcgaaat tcaatggtgg tctctcagat
     9841 gcaagaaact atggattatt aaaatcttca ggtaaatata tttcttttgt tgattcagat
     9901 gatgttgtta atagaggttt tataaaggaa atcatcaatt tcattgatgc ctatgatttt
     9961 gatatattgt catttgactt catgaagttt tttaataata atgatgctct tattctttca
    10021 caaatagatg aattctccaa aatggcagaa agagtagatt cggagttcta caagtccaag
    10081 cctgtttttg cttggaataa agtttatcga cgttctcttt tcgacaatga agtatttcct
    10141 aaggggtggt attatgaaga tgttgcatta attccgttat tattagatag agcaaaagtt
    10201 ttataccata ttaatagcgt ttgttatttt tatagacaga gacaaggagc aatcactttt
    10261 ttttatgaca ataaatatct tgatatttta aaaggagtgt cttttttata tgacaggtca
    10321 caatcttcat tcattaaaac gataattata aatcagtttt ttactttaac cttactatct
    10381 ttaagattgc cgactgataa ttatttcatg aatatgaggg ggattattga tacctattgt
    10441 gaaaaatttg acttggattc ctttgagcct aaactaatat taaaacacat tcctttttta
    10501 ttgcttaaaa aattgaagtc gctgtgtgtt tatccactgt atttatttaa acctgcagtt
    10561 tttttacata aacaaattaa acattttaga ggaaaactaa agtgaaatat gattatataa
    10621 ttataggtag tggtcttttt gggagtgtgt ttgcacatga attagggaaa atgaaaaaaa
    10681 aggtcaaggt tattgagcgt cgtgatcatg ttggtggtaa tgtatattgt gagaatattg
    10741 aaggaattaa tgttcataaa tatggcgcac atatatttca tactaacgat aaagaaatat
    10801 gggattatgt aaatcaattt gtagagttta atagatatac aaattcacca ttagcttttt
    10861 caaaaggtaa aatttataat ttacctttta acatgaatac ctttaatcaa ctctgggggg
    10921 ctattactcc tcaacatgcg aaagaaatta ttaaaaagca aagcggtgaa ataagtggaa
    10981 ataaaccaag gaatcttgaa gagcaagcta tttctttggt gggacgagat atttataatt
    11041 gtttgattaa ggagtacact gagaaacaat ggggacgacc ttgcactgaa ttgccatcat
    11101 ttattataaa acgactccct gttaggttta catacgataa taactatttt aatgaccagt
    11161 atcaaggaat accagtcggt ggttacaata aattaatcga tgcattatta aatggtattg
    11221 aagttcaaac atgcactgac tattttgaag agaagaaata ttgggattct attgcaaaga
    11281 aaattattta tacagggcct attgataagt tttattgcta taaatttgga gctttggatt
    11341 atcgatcatt aaaatttgag catgaattac tgaatgtcga taattttcaa gggaatgctg
    11401 ttgtaaatta tatagacaaa aaattcccat tcactcgtat aatagaacat aaattttttg
    11461 aatttggtga acagcataat actctcatta ctcgagagta cccaaaagaa ttcgaagaag
    11521 gcgatgagta ttactatcct gtcaatgatg aaaaaaatat gcaaatctat aaaatgtatt
    11581 ataatctttc taaacaggaa agtaatgtga tttttggtgg acgtcttgcg gaatataaat
    11641 attatgacat gcatcaagtt atcaaatcag ctttaaatgc attccgaaaa gaggtacgtt
    11701 taggtgaata aagaagtaat cgcagttatt gtaacctata atagaaaagc attattgctg
    11761 aaagttatag atgctgttat taaccaaagt tatccactaa aaaaaatatt gataatagat
    11821 aataatagta ctgatggtac ggagatgttt atttcaaata ggctctctga cgtggtgaag
    11881 tataaaaata cgggggataa tttagggggg gctggcgggt tttatagagg attcatagag
    11941 gcggaaaaat atggctatga ttatctttgg ttaatggacg atgattttat gcctacagtg
    12001 aattgtcttg aaatattaat atcaaatagc cctgatggta ttgtccaacc tgttagatat
    12061 aacttggatg aaacttgtgc agagctttct ccattaactt atgatttatc taacccattt
    12121 aagttaaacc ctaaaggtac accattaaaa aattacttaa atacaattgg taataaatct
    12181 gcgaaggtag atattgaagc tattccattt gaaggtccat tgatttcacg aaaggttgtt
    12241 gaaaaaatag gctatccaga gcctaagttt tttatttttt gtgatgacat tgaatacgct
    12301 attaaagcta aaagaaaggg gattcccatc cagtgcaatc ttaaagccaa ggcataccgc
    12361 cttttggtca acaatcaagg taatgatctt ctttcctgga aaggatattt tatgcttcga
    12421 aatcttttct acctgcataa aacgtatggg accaatttcc ttgttagaca aaaacctatc
    12481 gtattgggat taggatatgc attgtcgtgt gtattgaaat gcaatttttc tcaacttccc
    12541 gttatatggc gtgctttttg ggattcatct acgttaagaa ataccgaaaa attcaggccc
    12601 gaaatgagaa gtaaataatt tattattatg tttttattaa ttaatgtcga tttttactaa
    12661 aattcttaac acgatattta tccctgacag gagtaaacaa tgtcaaagca acagatcggc
    12721 gtcgtcggta tggcagtgat ggggcgcaac cttgcgctca acatcgaaag ccgtggttat
    12781 accgtctcta ttttcaaccg ttcccgtgaa aaaacggaag aagtgattgc cgaaaatcca
    12841 ggcaaaaaac tggttcctta ctatacggtg aaagagtttg ttgaatctct ggaaacgcct
    12901 cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat tgattctctc
    12961 aagccatacc tcgataaagg tgacatcatc attgatggtg gtaacacctt cttccaggac
    13021 accattcgtc gtaaccgtga gctttctgca gaaggcttta acttcattgg taccggtgtt
    13081 tccggcggtg aagagggggc gctgaaaggg ccgtccatca tgcctggtgg ccagaaagaa
    13141 gcctatgaac tggttgcacc gatcctgacc aaaatcgccg cagtagctga agacggggag
    13201 ccatgcgtta cctatattgg tgccgatggt gcaggtcact atgtgaagat ggttcacaac
    13261 ggtattgaat acggtgatat gcaactgatt gctgaagcct attctctgct taaaggtggc
    13321 ctgaacctca ccaacgaaga actggcgcag acctttaccg agtggaataa cggtgaactg
    13381 agcagctacc tgatcgacat cactaaagac atcttcacta aaaaagatga agacggtaac
    13441 tacctggttg atgtgattct ggatgaagca gcaaacaaag gtaccggtaa atggactagc
    13501 cagagcgcgc tggatctcgg cgaaccgctg tcgctgatta ccgagtctgt gtttgcacgt
    13561 tatatctctt ctctgaaaga tcagcgcgtt gccgcgtcta aagttctcac tggcccgaaa
    13621 gcacagccag ctggcgaaaa ggctgagttc atcgaaaaag ttcgtcgtgc gctgtatctg
    13681 ggcaaaatcg tttcttacgc tcagggcttc tctcagctac gcgccgcgtc tgaagagtac
    13741 aactgggatc tgaactacgg cgaaatcgcg aagattttcc gtgctggttg catcatccgt
    13801 gcgcagttcc tgcagaaaat caccgatgcc tatgccgaaa atccgcagat cgctaacctg
    13861 ctgctggctc cgtacttcaa gcaaattgcc gatgactacc agcaggcgct gcgcgatgtc
    13921 gtcgcttacg cagtacagaa cggtatcccg gttccgacct tcgccgctgc ggttgcctat
    13981 tatgacagct accgttctgc tgttct
//
