LOCUS       GU299794               10140 bp    DNA     linear   BCT 30-SEP-2010
DEFINITION  Escherichia coli strain G1216 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299794
VERSION     GU299794.1  GI:288816233
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Liu,B., Perepelov,A.V., Li,D., Senchenkova,S.N., Han,Y.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Structure of the O-antigen of Salmonella O66 and the genetic basis
            for similarity and differences between the closely related
            O-antigens of Escherichia coli O166 and Salmonella O66
  JOURNAL   Microbiology (Reading, Engl.) 156 (PT 6), 1642-1649 (2010)
   PUBMED   20185508
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Liu,B., Perepelov,A.V., Li,D., Senchenkova,S.N., Han,Y.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1216"
                     /db_xref="taxon:562"
                     /note="serogroup: O166"
     misc_feature    1..10140
                     /note="O antigen gene cluster"
     gene            1175..2428
                     /gene="wzx"
     CDS             1175..2428
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54955.1"
                     /db_xref="GI:288816234"
                     /translation="MSGLNIKRLFANDIVINIIWLVLEKLYFIILVFLCEGLISRTLG
                     VSDYGKWIYAINTIIIISSIALVAGSEVMVPALSKHKKIVGELLSSAFIIRMLGALTS
                     FFFINIYIFFAIKDEVLQQMLMSLSLILLFNEPFGVITNYCQSRINMKGIVLARCVSL
                     GVRTIFVLVSFKLLDNKFIYFSRAAESISLAIILTMLFLSYKIKIKLSKKIILIIFNR
                     GISFWLPLICMMVYFRVDRYFVEKYFSYDTLAMYGVAVQFIEQAFLLIVIIIQSISPR
                     YIFPILSKRDLIENIKKITLILFLVVIFMQVFSYLFLPFIITLVFGHEYFNAGQIAVT
                     LLPSLLFYAIDSILMQVLYKDKNAIAILFKWLSMMMISCSLYYVWYGLLSEMNMAMIF
                     NINYVIMTAITFLFVKKGLKVRDYD"
     gene            2421..3230
                     /gene="weiA"
     CDS             2421..3230
                     /gene="weiA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiA"
                     /protein_id="ADC54956.1"
                     /db_xref="GI:288816235"
                     /translation="MINFTIFTPTYNRADTLERCYRSILLQKRIDIEWLIIDDGSTDN
                     TKFLVEKFMAEGKIRIVYIYQNNAGKQVAWNVAIQKANGQYFIGLDSDDALYEDVLSV
                     LFPKINEINADSSIVGLRCLSQKVPKGSNRIKDFSSGHDSIHYWYDEFASGISQERID
                     ILKTKIIKEYPYPVQDGVKFIPEVWFYSTISKKYKFYYVNKVVRVFYDDLSTNRLSRS
                     SFKKHFKGHLIARKAMLHDIPIIYFLKNPIGLFKTITRYLQACICNFFYYK"
     gene            3319..4392
                     /gene="wzy"
     CDS             3319..4392
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54957.1"
                     /db_xref="GI:288816236"
                     /translation="MYHIAIALPLLFFVTGVFYRKLTNILVFFTILTIGTIVSFRDIS
                     VDRDSIVYFTVFNDLSNINSLYELWIFSLSYGQEIGFMLLMKLFHLFGASYYVFRGCF
                     EFLTLFFIWSFIKKTCDDLFYLIAFFIYISMFLYFRDFTQIRFSFSCVMALNSIYYRL
                     KNNNSRSIAYFIAGLLVHNSILIIAPILLLIKFKFIYTAKFYFSSIVVALLLFKINIF
                     GVLINTGYLPHQITRYINQDEMMGNTFGFTAIITIVVSFLMCLRFREYYGIDYKLLYL
                     NMLFSSITALVFMNIPIMMRMQLLFLTAIIILPNVIYTYWIRKDSANRLLLKIFFVSV
                     CIIYYYSVLSTGIVSGYKTFFSI"
     gene            4403..5485
                     /gene="weiB"
     CDS             4403..5485
                     /gene="weiB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiB"
                     /protein_id="ADC54958.1"
                     /db_xref="GI:288816237"
                     /translation="MKNICFFVGDISRSGGTERVTCKLANYLTLNGFDVSIISIYGTN
                     SAFFPLDEKVKKYKLFDRYLKGTLSFPLIIFRLRKLLKKKCIHKLISVESMLALYSLP
                     ATFNLPIENICWEHFNFKINLGKKTRNIARRMAAEYCSYVITLTDRDKAYWENNLKCK
                     AIIKRIYNPIPFDNMYLPKFEKKKRIAIAVGRLTHQKGFDVLIKIWADLYHYNANWTL
                     KIIGGGEEEHSLKTFCQNHDISNIEFISVTDNIQDYYSEASLYLMTSRYEGFPMVLLE
                     AQEYGIPVLAFDCETGPSELILHNKTGWIINNNNVREFEERLIYALNLFEKDHDTYNK
                     MCHEAFLNSKNYHIENIGSQWINILK"
     gene            5496..6587
                     /gene="weiC"
     CDS             5496..6587
                     /gene="weiC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiC"
                     /protein_id="ADC54959.1"
                     /db_xref="GI:288816238"
                     /translation="MKNICLITTGLGMGGAERQVCDLANSFYDKGNNVIIISLNNEII
                     NSPANENIIVYSLKLKKSFVGFFRAILTARKILKKYSPDVIHSHMFHANIFSRILRLI
                     YPKVPLICTAHNTNEGGRLRMFLYRITDFLATISTNVSQEAVESFVQKGAVKRGRMIA
                     FYNGIDTNLYKYSPSYRINKRKELNLNDEDVLLLAVGRLTDAKNYKNMLKALQIVIEN
                     KRVHLAIIGNGPDRNKLIDYASRLGITQNVHWLEVRFDVYEWMSAMDLYVMSSSWEGM
                     PLVICEAMSCGGIVVATDCGGVKEIVGNSGFIVPKNDSKALSKSISDALALTEEERVS
                     ITSSARDRIERYYSLNIISGKWLSLYDEF"
     gene            6574..7650
                     /gene="weiD"
     CDS             6574..7650
                     /gene="weiD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiD"
                     /protein_id="ADC54960.1"
                     /db_xref="GI:288816239"
                     /translation="MTNSDTSDLYNNQKNIVFVITKSEVGGAQTWVSQLKFILEEKHN
                     IFLITSETGWLTDYFEPEKVKIVPGLVSMLKPGAIFKISSALKEFNADLVISNSATAG
                     LYSRLAKIFYRHKHIYVSHGWSCIYNGGRFEKIFCLIERFLSLLTNKVLCVSINDQYN
                     ATHVIGINADKTVVIRNSINPMEKKNFINKKFKILFVGRMSYPKRPDLLAEAAVYFPD
                     VVFDFVGGGPLLSELKKKYQSSPNIRFLGEIGSFKNFKDYELFVLASESEGLPMSALE
                     AATAGLPLLLSDVGGCNELILESITGDKNGLLFNNKLSELVSQIREIINNYELFYYAA
                     NDISDKFDINKHKDYYYQLIDNLC"
     gene            7699..8712
                     /gene="gne"
     CDS             7699..8712
                     /gene="gne"
                     /note="UDP-GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADC54961.1"
                     /db_xref="GI:288816240"
                     /translation="MAILITGGAGYIGSHTAVALLKKNYEVVIIDNLVNSHYSVIEKI
                     QKITGKTIKFYEGSISNKSLLHEIFSKNRIESVLHFAGLKSVAESTRLPLHYYNTNVS
                     ATLNLVEAMLKNDVCNFIFSSSATVYGEPENIPLNESSRVGNTTNPYGTSKLMVEKIL
                     QDISSSNQKFRTTILRYFNPVGAHKSGEIGEDPNGTPNNLMPYICQVAIGKRKELFIY
                     GNDYKTKDGTGIRDFIHVMDLAEGHIAALKKCDNGSNYKIFNLGTGKGYSVLDLISAF
                     ERVTSVTIPYVITSRRPGDIAECWSDPKKAFLELGWKAMYNLDDMVRDAWNWQKKNPS
                     GYK"
BASE COUNT     3245 a   1571 c   2013 g   3311 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtgactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gagtctctcc ttgaacagcg cgtgaagcgt
      121 caactgttgg cggaagtgca gtctatctgt ccgcctggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttatgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgcagccat gattgcgcgc ttcaacgaaa cgggacgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agagccgctg
      421 gatcgtgaag gcaaagtcag ccgcattgtt gaatttatcg aaaaacctga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttggac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgct
      601 gaactggcga aaaaacagtc cgttgatgca acgctgatga ctggggacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcattt gtgaagtatg gattacgcaa cctgaaagaa
      721 ggtgcgaagt tccgcaaagg tattgagaag ctgttaagcg aataaagata tgacctagtg
      781 tagtggctga taaggcaatt atggcggcgg aggagctcat ttagtgtaaa tattcgtgcc
      841 gtatttttat atggatatgc agtataacaa cgattaataa aaggattctg atcacaaggt
      901 ttcgcggaaa tgattgttct aaaagttaac aatagctaat cagtaagaga atctagggat
      961 aattttagag gaataggagt atatacaatt cgttggaagt gcgtagcgtg ctggtagctt
     1021 taaagccagg ggcggtagcg ttgtattctt cgaataatta atcgcaactg tgtgtaatga
     1081 gtcgttcgtt tacgtttaat gaatgttaaa aaagttagag tcgttcatat tgaacacaga
     1141 aaaatgaatt cttactggtt aataaaaata aattatgagt ggtttgaata taaaaaggtt
     1201 atttgctaat gatattgtta ttaatatcat atggttagtt ttagaaaagt tatatttcat
     1261 cattctcgtt ttcttatgtg agggcttaat tagcagaact ttaggcgtta gtgattatgg
     1321 gaagtggatt tacgcaatta acaccattat cataatatct tcgatcgctt tagttgcagg
     1381 gagtgaagtt atggtccctg ccttaagtaa acataaaaaa atagtcggag agttgttaag
     1441 ttctgcattt atcattagaa tgttaggggc actaacttca ttttttttta ttaacatata
     1501 tatttttttc gcgatcaagg atgaagtttt acaacaaatg ttgatgtcct tgtcactaat
     1561 tcttttgttt aatgaaccgt ttggcgttat tacaaattat tgtcaatcac gaattaatat
     1621 gaaaggtatt gtgttggcaa gatgtgtatc cttaggagtg agaacaatat ttgtgcttgt
     1681 ttcatttaaa ttattagata ataagtttat atatttttca agagcggcag aaagcatttc
     1741 tttagctatt atattaacaa tgcttttttt aagttataag attaaaataa aactctcaaa
     1801 aaaaataatc ttgataatat ttaatcgtgg gatttcattt tggctccctc taatatgcat
     1861 gatggtttat tttagagtgg atcggtattt tgttgagaag tatttttcat atgatacatt
     1921 ggctatgtat ggagttgcag ttcagtttat tgaacaagca tttttattaa tagttatcat
     1981 aatacaatct atatcaccta ggtatatatt ccctatatta tctaaacgtg atttgattga
     2041 gaacataaaa aaaatcacat taattctttt tttagtagtg attttcatgc aagtgttttc
     2101 ttatttgttt ttgcctttta ttataacatt agtttttggt catgaatact tcaatgccgg
     2161 acaaatcgca gtaactcttt taccatcatt actgttttat gcaatagatt caattttaat
     2221 gcaggtatta tataaagata aaaatgctat agctatttta ttcaaatggt tatcaatgat
     2281 gatgatttct tgttcattat attatgtatg gtatggcctg ttatcagaaa tgaacatggc
     2341 aatgatattt aatataaatt acgttataat gactgctata acctttcttt ttgttaaaaa
     2401 aggactaaaa gttagagact atgattaact ttacaatttt tactcccaca tataatagag
     2461 cggacacatt agaaagatgt tatcgaagca tcctacttca gaaacgaata gacatagaat
     2521 ggctaattat tgatgacggt tctactgata atactaagtt tttagtagaa aaatttatgg
     2581 ctgaagggaa gattagaata gtatatattt atcagaacaa tgcaggaaaa caagttgctt
     2641 ggaacgtcgc gatacaaaag gctaatggtc agtattttat aggacttgat tctgatgatg
     2701 ctttatatga ggatgtatta tcagttctat ttccaaaaat aaatgaaata aatgcagatt
     2761 caagtattgt cggactacgt tgtctgtcac aaaaagttcc aaagggaagc aatagaatta
     2821 aagatttttc atcaggacat gattcaatac attactggta tgatgaattt gctagtggta
     2881 tttcacaaga aagaattgat atattgaaaa caaagatcat taaagaatat ccgtatcctg
     2941 ttcaagatgg tgttaaattt ataccagagg tatggtttta cagtacgata tcaaaaaaat
     3001 acaaatttta ctatgtaaat aaagttgtta gagtatttta tgatgacctt agtactaata
     3061 ggttaagccg aagttctttc aaaaagcatt tcaaaggcca tttaattgca cgtaaagcta
     3121 tgttgcacga tatacctata atatactttc taaaaaatcc tattggttta tttaaaacaa
     3181 ttacgagata tctccaggca tgtatatgca atttttttta ttataaataa taatctttca
     3241 aacatgtttg ttactaagat caatatgtta tagcattatg caaataaaca tatactttaa
     3301 atattataag agattagtat gtaccatata gcaatagcgt tacctttatt attttttgtt
     3361 actggagttt tctatagaaa attaacaaat atattggttt tttttacgat tttaaccata
     3421 ggaacaatag tttcgtttcg agatataagc gttgatcggg atagtattgt ttattttaca
     3481 gtatttaatg acctttctaa tattaactca ttatatgaat tatggatatt ttcactctct
     3541 tacggtcaag aaattggctt catgttatta atgaagttgt tccatctatt tggtgcctcc
     3601 tactatgtat tccgtggttg ctttgagttt ttaactttat tttttatttg gtcctttata
     3661 aaaaaaactt gtgatgattt attctatctt attgcatttt ttatatatat atcaatgttt
     3721 ctgtatttca gagattttac gcaaataaga ttttcattta gttgtgtgat ggcattaaat
     3781 tccatttact atagattgaa aaataataat tctcggtcta ttgcttattt catagctggc
     3841 ctccttgttc ataattctat tctaataata gcgccaatct tactgctaat aaaatttaaa
     3901 ttcatttaca ctgccaaatt ttatttttcg agcatagtgg ttgcattatt gttatttaaa
     3961 attaatatat tcggcgtgct aattaataca gggtatctcc cacatcaaat aacaagatat
     4021 attaatcagg atgagatgat gggaaatact ttcggcttta ctgcgataat taccattgtt
     4081 gtttcatttt taatgtgctt acgatttaga gagtattatg gtattgacta taaacttcta
     4141 tatttaaata tgttgttctc aagtattaca gctttagttt ttatgaatat tccaataatg
     4201 atgagaatgc agttgttatt ccttacagca ataattatat tgccaaatgt aatctatact
     4261 tattggataa gaaaggattc ggcgaataga cttttactga aaatattctt tgtgtctgtc
     4321 tgtattattt attattactc agtgttatca actggaattg tatcagggta taaaacattt
     4381 tttagtattt aagagggtta ttgtgaaaaa tatttgtttt ttcgtcggag acataagccg
     4441 gtcggggggg acagaaagag ttacatgtaa attagctaat tatcttacat taaatggctt
     4501 tgatgtttct atcataagta tttacggtac aaatagtgcc ttttttccgt tagatgaaaa
     4561 agtaaaaaaa tataaactct ttgataggta tttaaaagga actttaagct ttccattgat
     4621 aatatttaga ctaaggaaat tattgaaaaa gaaatgtata cataaactaa tttcagttga
     4681 atcaatgctt gctttatact cgctaccagc aacttttaat ctaccgattg agaatatttg
     4741 ttgggaacat tttaatttta aaattaattt aggaaaaaaa acgagaaata tagctaggcg
     4801 aatggccgcc gaatattgta gctatgtcat tacattaaca gacagagata aagcttattg
     4861 ggagaataac ttaaagtgta aagcgataat aaaaagaatt tataatccta tcccctttga
     4921 taatatgtat ttgccaaaat ttgagaaaaa aaaacgaata gctatagctg ttggtcgatt
     4981 aactcaccag aagggatttg atgtgctaat taaaatttgg gcggacttat atcattataa
     5041 tgccaactgg actttaaaaa taataggtgg cggtgaggaa gaacacagtt taaagacatt
     5101 ttgccaaaat catgatatta gcaatataga atttatttcc gtcacagata atatacagga
     5161 ttattatagt gaagcctcat tatatttgat gacatctaga tacgaagggt ttccgatggt
     5221 acttttagag gcgcaggaat atggtatacc tgtattagct tttgattgtg agactggccc
     5281 tagtgagtta attttgcata ataagactgg ttggataata aataataata atgtgcgtga
     5341 gtttgaagaa agattaattt atgcgttaaa tttatttgaa aaagatcatg atacctacaa
     5401 taaaatgtgt catgaagcat tcttaaactc aaagaattat catatcgaga atataggttc
     5461 tcagtggatt aatatcttga aataaaggga ataatatgaa aaatatttgt cttattacaa
     5521 ccggccttgg tatggggggg gccgagagac aagtttgtga cctggcaaat agtttctatg
     5581 ataagggaaa caacgtgatt ataataagct tgaataatga aattataaat tctcctgcta
     5641 atgaaaatat aatagtttat tctctgaaat taaaaaaatc tttcgttggt ttttttagag
     5701 ctattcttac agcaagaaaa attttaaaaa aatattcacc tgatgttatt catagtcata
     5761 tgtttcatgc gaatattttt tcacggatac taaggttgat atatccaaaa gtccctctga
     5821 tttgtacggc gcacaataca aatgaaggtg gcagactgag aatgttcttg tacagaatta
     5881 ctgatttttt agcaacaatt tcaacaaatg ttagccaaga agccgttgaa agctttgttc
     5941 agaagggggc tgtcaaaaga ggcagaatga ttgcatttta taatgggata gatacgaatt
     6001 tatataaata ttctcccagt tatagaatta ataaaagaaa agaattaaat ttaaacgatg
     6061 aggatgtatt attactagcc gttggtcgat taactgatgc gaaaaattat aaaaatatgc
     6121 taaaggcact gcaaatagtt attgaaaata aacgtgtgca tctcgccatt ataggtaatg
     6181 gtcctgatag aaataaactc atagattatg cttctcgatt aggaataacc caaaatgtgc
     6241 attggttaga agttcgtttt gatgtttatg aatggatgtc agcgatggac ttgtatgtta
     6301 tgtcttcttc atgggaaggt atgcctttag ttatatgtga ggctatgtct tgtggcggaa
     6361 ttgttgtagc gacagattgc ggaggagtta aagaaatagt tggcaattct ggctttattg
     6421 tgccaaaaaa tgattccaaa gcattatcaa aatcaatatc cgatgcatta gctcttacag
     6481 aggaagagag agtttcaatc acatcttctg caagagatag gattgaacgg tactattcct
     6541 taaatattat ttcgggaaaa tggttaagtt tatatgacga attctgatac tagcgacctg
     6601 tacaataatc aaaaaaatat tgtgtttgtt attactaagt ctgaagtcgg tggggcccaa
     6661 acttgggttt cacaacttaa atttatactt gaagagaagc ataatatttt tctaataact
     6721 tcagagactg gatggcttac agattatttt gaacctgaaa aagtaaaaat tgtacccggc
     6781 ttagtcagta tgttaaaacc gggggcaatt tttaagataa gttctgcatt aaaagagttc
     6841 aatgcagacc ttgtgatatc taattctgct actgctggac tgtattctcg acttgcaaaa
     6901 atattctata ggcataaaca tatatatgta tctcatggat ggtcttgcat atataatgga
     6961 gggcggtttg aaaaaatatt ttgcctgata gaacgctttt tatcgctttt gacaaataag
     7021 gttctatgtg tttctataaa tgatcaatat aatgcaaccc atgtcattgg aataaatgca
     7081 gataaaaccg ttgtcattag aaactcgatt aatcctatgg agaaaaaaaa cttcattaat
     7141 aagaaattta aaattttgtt tgtaggccga atgtcttatc ctaaaagacc ggacttgtta
     7201 gctgaggctg cggtatactt tcctgatgtg gtttttgatt tcgtcggtgg tggtccctta
     7261 ttatcagagc taaaaaagaa ataccaatct agccctaata ttagattttt gggtgaaatc
     7321 gggagtttca agaattttaa agattacgaa ttgtttgtat tggcctcaga aagtgagggc
     7381 ttacctatgt cagctcttga ggccgctact gctggattac cattattatt gagtgatgtc
     7441 ggcggatgca atgaattaat ccttgaaagt attacaggag ataaaaatgg acttttattt
     7501 aataataaat taagtgaact tgtgtctcag ataagagaaa taattaataa ctatgaactt
     7561 ttttattatg cagctaatga tatcagtgat aaattcgaca ttaataaaca taaggattat
     7621 tattaccaac tgattgataa tctttgttaa caactattta ttattacttt atctaatctt
     7681 aaattcgaga gagtgcttat ggccatattg ataacaggtg gcgcagggta tattgggtcg
     7741 catactgctg ttgcattact gaaaaaaaat tatgaagtgg taattattga taatttggta
     7801 aattctcatt attctgttat tgaaaaaatt caaaagatta ctggaaaaac aataaagttt
     7861 tatgaaggaa gtattagtaa taaaagttta ttacacgaga ttttctcaaa aaacagaatc
     7921 gagtctgttt tacattttgc tgggttgaaa tccgttgcag aatctactcg acttcctcta
     7981 cattattata atactaatgt ttcagcaact ttaaacttag tagaggcaat gctaaaaaat
     8041 gatgtttgta attttatttt cagctcttct gcaactgtat atggagagcc agagaatata
     8101 ccgctaaacg aatcttcgcg agtcggtaat acaacaaatc cttatggaac atcgaagtta
     8161 atggttgaaa aaatactaca agatatctca agctctaacc aaaaatttcg tacgactata
     8221 ttaagatatt ttaatccagt gggagctcac aaatccggtg agattgggga agatcctaat
     8281 ggtacaccaa ataatttgat gccttacata tgtcaagtcg ccattggtaa gcgaaaggaa
     8341 ttatttattt atggtaatga ttataaaaca aaagatggga cagggattcg tgatttcata
     8401 catgtaatgg atcttgcaga ggggcatatt gctgctttga aaaaatgtga taatggaagc
     8461 aactataaaa tttttaattt aggtacagga aaaggatatt ccgtactcga tcttatttca
     8521 gcttttgagc gtgttacatc tgttaccata ccatatgtca taacatcaag acgtccaggt
     8581 gacattgctg agtgttggtc agaccccaaa aaagccttct tagaattagg ttggaaggcg
     8641 atgtataact tggacgatat ggtaagagat gcatggaatt ggcagaaaaa aaatcctagt
     8701 ggttataaat aaaaatacta ttatcggttc ataacggatt tatattaatc tcagcaatgt
     8761 ttatgtattg tatgtgaaaa tcccctgaca ggagtaaaca atgtcaaagc aacagatcgg
     8821 cgtagtcggt atggcagtga tggggcgcaa cctagcgctc aacatcgaaa gccgtggtta
     8881 taccgtctct attttcaacc gctcccgtga gaagacggaa gaagtgattg ccgaaaatcc
     8941 aggcaagaaa ctggttcctt actatacggt gaaagagttt gttgaatctc tggaaacgcc
     9001 tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta ttgattccct
     9061 caagccgtac ctcgataaag gtgacatcat cattgatggt ggtaacacct tcttccagga
     9121 caccattcgt cgtaaccgtg agctttctgc cgaaggcttt aacttcatcg gtaccggtgt
     9181 ttccggcgga gaagaaggcg cgctgaaagg tccttccatt atgcctggtg ggcagaaaga
     9241 agcctatgaa ctggttgcgc cgatcctgac caaaatcgcc gcagtggctg aagacggtga
     9301 gccatgcgtt acctatattg gtgccgatgg cgcaggtcac tatgtgaaga tggttcacaa
     9361 cggtattgaa tacggagata tgcaactgat tgctgaagcc tattctctgc ttaaaggtgg
     9421 cctgaacctc accaacgaag aactggcgca gacgtttacc gagtggaata acggtgaact
     9481 gagcagctac ctgatcgaca tcaccaaaga tatcttcacc aaaaaagatg aagacggtaa
     9541 ctacctggtt gatgtgatcc tggatgaagc ggctaacaaa ggtaccggta aatggaccag
     9601 ccagagcgca ctggatctcg gcgaaccgct gtcgctgatt accgagtctg tgtttgcacg
     9661 ttatatctct tctctgaaag atcagcgtgt tgccgcatct aaagttctct ctggcccgca
     9721 agcacagcca gcaggcgaca aggctgagtt catcgaaaaa gttcgccgcg cgctgtatct
     9781 gggcaaaatc gtttcttacg cccagggctt ctctcagctg cgtgctgcgt ctgaagagta
     9841 caactgggat ctgaactacg gcgaaatcgc gaagattttc cgtgctggct gcatcattcg
     9901 tgcgcagttc ctgcagaaaa ttaccgatgc ttatgccgaa aatccgcaga tcgctaacct
     9961 gctgctggct ccgtacttca agcaaattgc cgatgattac cagcaggcgc tgcgtgatgt
    10021 cgttgcttat gcggtacaga acggtatccc ggttccgacc ttcgccgctg cggttgccta
    10081 ttacgatagc taccgtgccg ctgttctgcc tgcgaactta attcaggcac agcgcgacta
//
