LOCUS       AB812070               14143 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 745-56.
ACCESSION   AB812070
VERSION     AB812070.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 14143)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="745-56"
                     /db_xref="taxon:562"
                     /note="serogroup:O170"
     gene            <1..690
                     /gene="wcaM"
     CDS             <1..690
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01872.1"
                     /translation="DNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARN
                     ITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVN
                     NIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQE
                     SSVGPALSMNFDMRKGVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIVNVE
                     KINFRLPERRE"
     gene            853..1758
                     /gene="galF"
     CDS             853..1758
                     /gene="galF"
                     /note="O170_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01873.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMMMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2092..3339
                     /gene="wzx"
     CDS             2092..3339
                     /gene="wzx"
                     /note="O170_02"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01874.1"
                     /translation="MKLNTTLNKNILYLAVVQGSSYILPLITFPYLVRVLGPELFGVL
                     GFCQASMQYLVLLTDYGFNWTATQQVAKNKKDIEKLTRIFWSVFFAKIFLASLAFIFL
                     AACCFLIEKYKELWVVLFSFSPLVLGNVIYPVWFFQGMEKMKWITICTITARCLVIPL
                     TFIFVKNGQDVWVAALIQGAVNLLAGLMGLCLIRKKRWVDKIIVDYNDIKQCLKDGWH
                     VFISTSAISLYTTSTTVILGFVSGPIAVGYFNVANTIRNAAQGLLNPITQAIYPRINA
                     MYDSDYPQAILLIKKTLRYVGALALLGSIFLYMLAPTIINIGVGYDYEQAISILRWMS
                     FLPFIIVLSNIFGVQTMLVHNFKKEFSRILVISGVTNLIIIYPLIRLFSGDGAAISLL
                     ITELMVSTLMYLFLRSKKIHLIY"
     gene            3351..4457
                     /gene="glf"
     CDS             3351..4457
                     /gene="glf"
                     /note="O170_03"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="BAQ01875.1"
                     /translation="MYDYLIVGAGLFGAVCAHELNKKGKKTLVIDRRDHIAGNAYTEN
                     NDGIQVHKYGAHIFHTNDKQIWEYVNSFVEFNRFTNSPLASYKNKLYNLPFNMNTFYQ
                     LWGVKTPTDAIDIINEQRKVLNGKVPGNLEEKAISLVGYDIYEKLIKGYTEKQWGRKA
                     TDLPAFIINRLPVRFTFDNNYFSDRYQGIPIGGYTNLVAKMLEGVEVNLGLDFLKDKE
                     YYSKLAKKIIFTGPIDEFFDYCFGMLEYRSLRFETETLDIDNFQGNAVINYTESTVPY
                     TRIIEHKHFDPVETLHTVITKEYPAEWCKGDEPYYPVNDVKNMSLFKKYKELADKSNV
                     IFGGRLGEYKYYDMHQVIRSALNCSNALIESDAK"
     CDS             4454..5386
                     /note="O170_04"
                     /gene="wfdK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01876.1"
                     /translation="MIKKKVIAIIVTFNRKNYLLRTINSLYQQTYKVDEIICVDNCST
                     DGTQKYLLDNDIIGRKNFHYYNTGANLGGAGGFKYGIDCLKDFEYEHVWLMDDDVELE
                     SNCLEILISSDHSSKGIVQPTRYYEDGEFFSYDYKYFNFKNPLREFKLGRVTSEDIEN
                     NDSIKIAAVPFEGPLIASDVINAIGNVNDEYFIIADDTDYSIRASRAGFYCFLIPEAK
                     LKRLIKPCSGQDPSWKVFFFHRNVTLVDKYYAPKYVLYIRACVRVMRYVITVSKRKKT
                     ISNFVAMIHGILAAMKNDITNNITDIQKKYADKK"
     CDS             5370..6176
                     /note="O170_05"
                     /gene="wbsG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01877.1"
                     /translation="MQIKNNINDLTIAISTYGERIVNAKKIALEFSRNGFRVDIIHQA
                     DNPDIIEEEIIDNKQIRYFYQNSKGVTKSRNYAIDVCTTKYIWFMDDDITFDYDNIHQ
                     LYGVLKKINCDNVSVCLTRIKDENGQPRKKYPQSGKIYNKRKIISVGTIEIIADATFL
                     KRNDIKFDENFGAGAAYPVGDEALFLNDVLDSNGSIYHFDLYFLFHPKDSSGIYRSKL
                     HFYSKGIVFRRLYGVIGLFYILYLILFRFNFFKEVSSLYLLKGYLVGGRR"
     gene            6176..7348
                     /gene="wzy"
     CDS             6176..7348
                     /gene="wzy"
                     /note="O170_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01878.1"
                     /translation="MRNILNSTIGIFIVFTIFFPLSPFVFSSGNAGALTTICSIIIIF
                     LLLQQKINVTNNLYMLLFPVLLSFIISALYWQEIMLLNYPKYLIFSIIIVSLLQKNIV
                     YIIGKYSTFVIVFALCCAVIGFIYAAIGGSAIYTITNPNGREFYFYLTTFTVTNYSGF
                     IRPSAFFDEPGALSFYICFVVMLREILWPRKRALNFALLAFGIVTLSLAHIVFTILYI
                     IYCLITKPTTKLIVGLVIILILSFVFLAVLPNKDVVFEYLISRSSSDTDGQGRSALLQ
                     AAIDTLQNAGVGLYIFGIDSNCLVNYDEACRYIYPRMGENILSPVVFGGLLTSWLFYF
                     SYIYVGYKSLRKFIPNFIIFSFLLLYLQRPYIYLLSYSFMFMIALRLSIGFFDKEK"
     CDS             7332..8186
                     /note="O170_07"
                     /gene="weuC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01879.1"
                     /translation="MIRKNKMSSRPLIDVVITSYNRLNLLLLALESVLFQKAHVNTII
                     IVDDNSSFTKDDFFDKAKKIIENDNTEIIFHKNDKNYGACYCRNLGANLSKAELIAFL
                     DDDDSWDPNHLNSLVDCFHEHVVLAYSGKNIINYQTGKIRRSLSKIQDDNQYEQMLRK
                     NYPGSTSSILVKKSALMAVNGFDVSLPAIQDYDFYLRICRLGLLKSSGMYTLNYRNDT
                     PLKITNQLQKARAAYNIIIQKYHGRERMILNRTIYKQNIIKCIRYFDFIYLFKFTRDF
                     INFIVEWR"
     CDS             8188..9105
                     /note="O170_08"
                     /gene="wfdK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01880.1"
                     /translation="MNERANVVAIVVTFKRKEFLCKILDSLIRQTFPINKIIVIDNNS
                     LDGTKELVEDFCRINTEVNRKIKYYDTGENLGGAGGFKCGFEIARNFDYSHLWLMDDD
                     LLPEDNCLEILLKCNIEGIVQPIRMNMDGTCAELSPINYDLKNPFRLRAKTKTVADIY
                     IKDKTPYLFDIHSVPFEGPLITKSVVEKIGFPNEKFFIFNDDLDYSLRARQKKFRICC
                     SSQAKATRLLKNNIKNDLSSWKGYYMLRNYFYIMRKYGDNFMVRLKPHWAIWIFIFYF
                     FLTGKKNINRIFSAYLDSKELTNSKKFRP"
     gene            9167..10573
                     /gene="gnd"
     CDS             9167..10573
                     /gene="gnd"
                     /note="O170_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01881.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAIAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10822..11988
                     /gene="ugd"
     CDS             10822..11988
                     /gene="ugd"
                     /note="O170_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01882.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            12078..13112
                     /gene="wzz"
     CDS             12078..13112
                     /gene="wzz"
                     /note="O170_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01883.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(13209..13820)
                     /gene="hisI"
     CDS             complement(13209..13820)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01884.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLVYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4271 a   2509 c   3049 g   4314 t
ORIGIN
        1 gataataact acccggaaga ccaggcagtg aaaaactttg tcgtggcgaa tatcacggga
       61 tcggattgtc gacaactgat ccatgttgaa aatggtaaac attttgttat tcgtaatatc
      121 aaagcccgca atatcacgcc ggatttcagt aagaaagcag gtattgataa cgccacagtc
      181 gctatttacg gttgtgacaa tttcgtgatt gataatattg aaatgattaa tagcgccggg
      241 atgttaatcg gctatggggt aattaaaggc aaatatctct cgataccgca aaatttccga
      301 gtgaataata ttcagctgga taacacccat cttgcttaca aattgcgcgg tatccaaatc
      361 tccgccggga atgctgtctc ctttgtggcg ctgactaaca ttgagatgaa gcgtgcgtcg
      421 ctggagttac ataacaaacc gcaacacctt tttatgcgta atatcaaggt gatgcaggaa
      481 tcctcagttg gaccagcatt gagcatgaac tttgacatgc gcaaaggcgt tcgcggcgtg
      541 tttatggcga aaaaagaaac actgctgtct cttgcaaatg ttcatgcggt gaatgaaaaa
      601 ggacaaagct ccgtcgatat cgacaggatt aatcaccata ttgttaatgt ggaaaagatt
      661 aactttagat tgccggaacg gagagagtag atttgcgacc attcctggaa aaatggagcc
      721 atacttagga acagtgctac tgcaatccac aacgaagcgg cgtaacatca caagtaattc
      781 agtcatcaat tcagggtaat tgatgctggc gaaaaaaatc gaacaagcta taattcagca
      841 accattttac aggtggatga aataatgacg aatttaaaag cagttattcc tgtagcgggt
      901 cttgggatgc atatgttgcc tgccactaag gcgattccca aagagatgct accgatcgtc
      961 gacaagccaa tgattcagta cattgttgac gagattgtgg ctgcagggat caaagaaatc
     1021 ctcctggtaa ctcacgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgag
     1081 ttagagtctc tccttgaaca gcgcgtgaag cgtcagctgc tggcggaagt gcagtccatc
     1141 tgtccaccgg gcgtgaccat tatgaacgtg cgtcagggcg aacccttagg tttaggccac
     1201 tccattttgt gtgcacgacc cgccattggt gacaatccat ttgtcgtggt gctgccagac
     1261 gttgtgatcg acgacgccag tgccgacccg ctgcgttaca accttgctgc catgattgca
     1321 cgtttcaatg aaacgggccg cagccaggtg ctggcaaaac gtatgccggg tgacctctct
     1381 gaatactcgg tcatccagac caaagagccg ctggaccgtg aaggtaaagt cagccgcatc
     1441 gtcgaattta tcgaaaaacc ggatcagccg cagacgctgg actcagatat catggccgtg
     1501 ggccgttatg tgctttctgc cgatatttgg ccggagcttg aacgcactca gcctggtgca
     1561 tgggggcgta ttcagctgac tgatgccatt gccgaactgg cgaaaaaaca gtccgttgat
     1621 gccatgatga tgactggtga cagctacgac tgcggtaaaa aaatgggtta tatgcaggcg
     1681 ttcgtgaagt atggactacg caacctgaaa gaaggggcga agttccgtaa agggattgag
     1741 aagctgttaa gcgaataatg aaaatctgac cggatgtaac ggttgataag aaaattataa
     1801 cggcagtgaa gattcgtggc gaaagtaatt tgttgcgaat attcctgccg ttgttttata
     1861 taaacaatca gaataacaac aagttagcaa taggatttta atcaaagttt tccaggattt
     1921 tccttgtttc cagagcggat tggtaagaca attagcgttt gaatttttcg ggtttagcgc
     1981 gagtgggtaa cactcgtcac atcgtaggca tgcatgcagt gctctggtag ctgtaaagcc
     2041 aggggcggta gcgtgtgtaa aaacacatta acattatttt gatggatacg tgtgaaatta
     2101 aatacgactt taaataaaaa tattttatat ttagctgttg tccaaggaag ttcatatatt
     2161 ctgccattaa ttacttttcc ttatttagtg agagtgttag ggcccgagtt atttggggtg
     2221 ctaggttttt gtcaggcatc aatgcaatac ctagtgttat taactgatta tggatttaat
     2281 tggacggcaa ctcagcaagt agctaaaaat aaaaaagata ttgaaaaatt aactagaatt
     2341 ttttggtctg ttttttttgc aaaaatattc ttggcaagtc ttgcgtttat atttttagct
     2401 gcatgttgtt ttcttattga aaaatataag gaactgtggg ttgtattatt ttcatttagt
     2461 cccttggtat tggggaatgt gatttatcct gtatggtttt tccaaggtat ggaaaaaatg
     2521 aaatggataa ctatatgtac tattacggct cgatgcttag tgataccttt aacattcatc
     2581 tttgttaaaa atggacaaga cgtatgggtt gctgccctta tccaaggggc ggttaactta
     2641 ttagcaggcc taatgggatt atgtttgatt aggaaaaaaa gatgggttga taagattatt
     2701 gttgattata atgatattaa acagtgctta aaggatggtt ggcatgtatt tatttcaaca
     2761 tcagctataa gtctttatac aacaagcact actgtgattc tcggatttgt ctcgggtcca
     2821 atagctgttg gttattttaa tgtggctaat acgatacgta atgctgctca ggggttactt
     2881 aacccaatta ctcaagctat atatcctcgc attaatgcta tgtatgatag tgactatccc
     2941 caggcgattc tattaataaa aaaaacgcta cgttatgtag gtgcattggc cttgttgggt
     3001 tcaatttttt tatatatgtt agccccaacg ataattaata taggtgttgg atatgattat
     3061 gaacaagcta tatctatact gcgctggatg tcttttttac catttattat tgttttaagt
     3121 aatatttttg gtgtgcaaac aatgcttgtt cataatttta aaaaagaatt tagcagaata
     3181 ttggtaatta gtggggtgac aaatcttata attatttacc cattaattcg tttattttct
     3241 ggtgatggtg cggctatttc actattaata acggaattaa tggtatcgac gttaatgtat
     3301 ttatttttac gctcgaaaaa aattcattta atttactaac aggagagcaa atgtacgact
     3361 atcttatcgt tggagctggt ttatttgggg cggtttgtgc tcacgaactt aataaaaaag
     3421 ggaaaaaaac gcttgttatt gatagaagag accatattgc aggtaatgca tataccgaaa
     3481 ataatgatgg cattcaggta cataagtatg gtgctcatat tttccatacc aatgataaac
     3541 aaatatggga atatgtaaat tcatttgtcg agtttaatcg gtttactaac tctcctctcg
     3601 cgagttataa aaataaactt tataatttgc catttaatat gaatacattc tatcaacttt
     3661 ggggtgttaa aacaccaaca gacgccatag atattatcaa tgagcaacgt aaagttttaa
     3721 atggtaaagt tcctggtaat cttgaagaaa aggcaatatc tctagtcggt tatgatattt
     3781 atgaaaaatt gataaaaggc tatactgaaa aacagtgggg cagaaaagca acagaccttc
     3841 ctgcatttat tattaatcga ttgccagtcc gctttacttt tgataataat tatttctctg
     3901 atcgttacca aggtattcct attggggggt atactaatct ggtagcaaaa atgcttgaag
     3961 gagtggaagt taatttaggt ttggattttt taaaagataa agaatattat tcaaaactag
     4021 ccaagaaaat aatattcaca ggaccgattg atgagttttt tgattattgc tttggtatgt
     4081 tagaatatag atcacttcga tttgaaactg aaacattaga tatagataat tttcaaggta
     4141 acgccgttat aaattataca gagtcaacgg ttccatatac aagaataatt gagcataaac
     4201 attttgatcc agtagaaact ttacacactg tgataacaaa agaatatcca gcagaatggt
     4261 gtaaaggtga tgagccttat tatccagtta atgatgttaa aaatatgagt ttattcaaaa
     4321 aatacaagga gttagcagat aaaagtaatg tgatttttgg ggggcgttta ggcgaatata
     4381 aatattatga tatgcaccag gttattcgct cggctttaaa ttgttctaat gcattaatag
     4441 aaagtgacgc aaaatgataa agaaaaaagt aatagctatt attgtcacat tcaacaggaa
     4501 gaattattta ttaaggacaa ttaactcatt atatcagcag acttataaag tcgatgagat
     4561 tatttgtgtg gataattgta gtacagatgg gacgcaaaaa tatcttctgg ataatgatat
     4621 tattggaaga aaaaatttcc attattacaa tacaggagca aatcttggtg gggcaggagg
     4681 atttaaatat ggaatagact gtctcaaaga cttcgaatat gaacatgtgt ggctcatgga
     4741 tgacgatgtc gaactcgaat ctaactgttt agaaatttta attagtagtg accattccag
     4801 taaaggaata gtacagccaa ctagatatta tgaggacggt gagttttttt cctatgatta
     4861 taaatatttt aactttaaga acccattgag ggagtttaag ttaggaaggg tcacctctga
     4921 agatatagaa aataatgaca gcattaaaat tgcagctgtt ccttttgaag gtcctttaat
     4981 tgcttcagat gttattaatg ctataggtaa tgtgaatgat gaatacttta tcatagctga
     5041 tgatacagat tattctataa gagctagccg agctggattt tattgtttcc tgatccctga
     5101 ggcaaaattg aagaggttaa taaaaccttg ttcaggtcaa gatccaagct ggaaagtatt
     5161 tttttttcac cgaaatgtaa ctttagtcga caaatattat gcacctaaat atgtgctata
     5221 tattagggct tgtgttaggg ttatgagata tgttattacg gtttctaaaa ggaagaaaac
     5281 gataagcaat tttgtggcta tgatacatgg gattctagca gcaatgaaaa atgatattac
     5341 aaacaacata actgacatac aaaaaaaata tgcagataaa aaataatatt aacgacttga
     5401 ctatagctat atcgacgtat ggcgaaagaa tagttaatgc gaagaaaatt gctttggaat
     5461 ttagtcggaa tggtttcaga gttgatatta tacatcaggc tgataaccca gatatcattg
     5521 aggaagagat aatagataat aaacaaataa ggtattttta tcagaacagt aaaggtgtaa
     5581 caaaaagtag aaattatgct atagatgttt gtacaaccaa atatatttgg tttatggatg
     5641 atgatattac ttttgattat gataatattc atcagttgta tggagtttta aaaaaaatta
     5701 attgtgataa tgtttccgtg tgtttaacaa gaattaaaga tgaaaatggt caacctcgga
     5761 aaaaataccc tcaaagcggc aaaatatata ataaaagaaa aattatatcg gttggtacta
     5821 tagagataat tgcagatgca acctttctca agaggaatga tataaaattt gatgaaaatt
     5881 ttggcgctgg agctgcttat ccagtgggag atgaagcgtt gtttttaaat gatgtattag
     5941 atagcaatgg ttcaatttat cattttgatt tatactttct ttttcatcct aaggattcta
     6001 gtggaatata tagaagtaaa cttcattttt acagcaaagg tatagttttt agacgactgt
     6061 atggtgttat aggattattc tatatattgt atttgatttt gttccgattt aattttttta
     6121 aagaggtgtc gtctttatat ttacttaaag gatacttagt tggtggaagg cgataatgag
     6181 aaatatctta aactcaacca ttggtatatt tattgttttt actatattct ttccattaag
     6241 tccgtttgtt ttttcgagtg gtaatgcagg tgctttgaca acaatttgct caataataat
     6301 aattttttta ttattacaac agaaaataaa tgtgactaat aatctatata tgcttttatt
     6361 tcctgttttg ttatcattta taatctcagc cttatattgg caagagatta tgttgttaaa
     6421 ttatcctaag tatttaatat tttccataat aattgtttct ttattacaaa aaaacatcgt
     6481 atatattatt ggtaaatatt caacatttgt tatagtattc gctttgtgtt gcgctgttat
     6541 tggatttata tatgcagcaa tcggcgggag tgcgatttat acaataacaa atcccaatgg
     6601 acgtgagttt tatttttatc taacaacatt tacggttact aattattctg gattcattag
     6661 accgtcagca ttttttgatg agcctggggc cttatcattc tatatatgct ttgtagtgat
     6721 gcttcgtgaa atattgtggc caagaaagag agcattaaat tttgctttac ttgcgttcgg
     6781 aattgttact ctaagtctgg cacatatagt gtttacaatt ctgtatatta tctattgtct
     6841 tattaccaaa ccaactacga aattaatagt tggtcttgta ataattctaa tattatcatt
     6901 tgtttttttg gcagtactac caaataaaga tgttgtattc gagtatttaa tttctcgttc
     6961 atctagtgat acagatggac aggggcgatc agcacttctt caagccgcaa ttgacacctt
     7021 gcaaaatgcg ggtgttggat tatacatttt tggaattgat tctaactgct tagtaaatta
     7081 tgatgaagca tgtcgctata tttatccaag aatgggagag aacattttat ctccagttgt
     7141 ttttgggggc ttattaacat catggctgtt ctatttttct tatatatatg taggttacaa
     7201 aagtttaaga aagttcattc ctaattttat aattttttct tttttattat tgtatttgca
     7261 acgaccatat atttacttat tatcatattc attcatgttt atgatcgcat tgcgactaag
     7321 tataggtttt tttgataagg aaaaataaaa tgagctctag gccattaata gatgttgtca
     7381 ttactagtta taatagattg aatctattgc tattagcttt agaatcagtc ctttttcaaa
     7441 aagctcatgt taatacaatc atcattgtgg atgacaattc atcatttaca aaagatgatt
     7501 tttttgataa agcaaaaaaa attattgaaa atgataatac tgaaataatt ttccataaaa
     7561 atgataaaaa ctatggtgca tgttattgca gaaaccttgg tgctaattta tccaaggccg
     7621 aattaatagc gtttctggat gatgatgata gctgggatcc taatcattta aattcacttg
     7681 ttgactgttt tcatgaacat gttgtacttg catatagcgg aaaaaatata attaattacc
     7741 aaacagggaa aataagacga tctttgtcaa agatccagga tgataatcaa tacgaacaaa
     7801 tgcttagaaa aaattatccg ggttcgactt cgtctattct tgttaagaaa agtgcattaa
     7861 tggcagtaaa tggttttgat gtgtcattac cagcaatcca ggattatgat ttttatctta
     7921 gaatatgccg tcttggacta ctaaaaagta gcggcatgta taccctcaat tataggaatg
     7981 atactccgct taaaataact aatcaattac aaaaagctag agcagcatat aatataataa
     8041 tccaaaaata tcatggtcgt gaaagaatga ttttaaatcg cacaatttat aagcaaaata
     8101 taattaagtg catcagatat tttgatttta tatatttgtt taaatttacg cgtgatttta
     8161 ttaattttat agttgaatgg aggtaacgtg aacgaaaggg caaatgtagt tgcaatagtt
     8221 gtcacattta aacgtaaaga atttttgtgc aaaatacttg attcgttgat tagacaaaca
     8281 tttcctataa ataagataat tgttattgat aataatagct tggatggtac taaagagtta
     8341 gtcgaagatt tttgtcgaat taatacggaa gttaacagaa aaataaaata ctatgatact
     8401 ggagaaaatt tggggggggc aggagggttt aaatgtggtt ttgaaattgc tagaaatttt
     8461 gattattcgc atctctggct tatggatgac gatttgttac cggaagataa ttgtcttgaa
     8521 attctactca aatgtaatat cgagggcata gtccagccaa ttagaatgaa tatggatggg
     8581 acttgcgctg aactttctcc aattaattat gatcttaaaa atccgtttcg tctacgagca
     8641 aagacgaaga cagtagcgga tatttacatt aaagacaaaa ctccatactt gtttgatata
     8701 cacagtgtac cttttgaagg cccattaatt actaaaagcg ttgtcgagaa aattggtttc
     8761 ccgaacgaaa aattctttat atttaatgat gacttggatt attcattaag agcacgtcaa
     8821 aagaagttta ggatttgttg ttcttctcaa gccaaagcaa cgcgtttact gaaaaataat
     8881 atcaaaaacg atctttcctc atggaaagga tattatatgc taaggaatta tttctatata
     8941 atgagaaagt atggggataa tttcatggtc agattaaaac ctcattgggc gatatggata
     9001 tttatttttt atttcttttt gactggaaaa aaaaatataa acagaatatt ttcagcttat
     9061 ttggattcta aagagttaac taactctaaa aaattccgcc cttaataggt taggtaaggg
     9121 tataatataa ttcatgttta tatacacccc ctgacaggag taaacaatgt caaagcaaca
     9181 gatcggcgtc gtcggtatgg cagtgatggg gcgcaacctt gcgctcaaca tcgaaagccg
     9241 tggttatacc gtctctattt tcaaccgttc ccgtgagaaa acggaagaag tgattgccga
     9301 aaatccaggc aagaaactgg ttccttacta tacggtgaaa gaatttgttg aatctctgga
     9361 aacgcctcgt cgcatcctgt taatggtgaa agcaggtgca ggcacggatg ctgctattga
     9421 ttctctcaag ccatacctcg ataaaggtga catcatcatt gatggtggta ataccttctt
     9481 ccaggacact attcgtcgta accgtgagct ttctgcagaa ggctttaatt tcattggtac
     9541 cggtgtctcc ggtggtgagg aaggcgcact aaaaggtcct tccattatgc ctggtggcca
     9601 gaaagaagcc tatgaactgg ttgcaccgat cctgaccaaa atcgccgcag tagctgaaga
     9661 cggtgagcca tgcgttacct atattggtgc cgatggtgca ggtcactatg tgaagatggt
     9721 tcacaacggt attgaatacg gcgatatgca gctgattgct gaagcctatt ctctgcttaa
     9781 aggtggcctg aatctttcca acgaagaact ggcgcagacg tttaccgagt ggaataacgg
     9841 tgaactgagc agctacctga tcgacatcac caaagatatc ttcaccaaaa aagatgaaga
     9901 cggtaactac ctggttgatg tgattctgga tgaagcagca aacaaaggta cgggcaaatg
     9961 gaccagccag agtgcgctgg atctcggcga accgctgtcg ctgattaccg agtctgtgtt
    10021 tgcacgttat atctcttctc tgaaagatca gcgtgttgcc gcatctaaag ttctctctgg
    10081 tccgcaagca cagccagcag gcgacaaggc tgagttcatc gaaaaagttc gtcgtgcgct
    10141 gtatctgggc aaaatcgttt cttacgccca gggcttctct cagctgcgtg ctgcgtctga
    10201 agagtacaac tgggatctga actacggcga aatcgcgaag attttccgtg ctggctgcat
    10261 catccgtgcg cagttcctgc agaaaatcac tgatgcatat gccgaaaatc cgcagatcgc
    10321 taacctgctg ctggctcctt acttcaagca aattgccgat gactaccagc aggcgctgcg
    10381 cgatgtcgtt gcttatgcag tacagaacgg tatcccagtt ccgaccttcg ccgctgcgat
    10441 tgcctattat gacagctacc gtgccgctgt tctgcctgcg aacctgatcc aggcacagcg
    10501 tgactatttc ggtgcgcata cttataagcg cattgataaa gaaggtgtgt tccatactga
    10561 atggctggat taatctgatt taaatcaatt aaccaacaca aggcccggag caaccctccg
    10621 ggctttttta ttatacaaag cggcaggtta tggccttttt ttataattaa tggttaaaaa
    10681 agcgatataa tacagagtcg cacagcagga tcgttgcctt gacagttcat ctacatcagc
    10741 gttaaaaatc ccgcagtaga tgaagttgtg gtggtggatt aatgaccact ctaaatgttt
    10801 aaccggaaga agtcagagct aatgaaaata acaatttcag gaacaggtta tgttggtctt
    10861 tcaaatggta ttctgattgc gcaaaatcac gaggtggttg cactggatat cgttcaggcc
    10921 aaagtggaca tgcttaacaa gaagcagtca ccgattgttg ataaggagat tgaagagtat
    10981 ctggcgacta aagatctcaa tttccgcgct acgacagata agtatgacgc gtataaaaat
    11041 gccgattacg ttattattgc cacgcctacc gattatgacc cgaaaacaaa ttattttaat
    11101 acctcaagcg tggaagcggt cattcgcgat gtgacagaaa ttaatcccaa cgcggtaatg
    11161 gtgattaaat ctacaattcc ggtaggtttc accaaatcaa ttaaagaacg tctgggtatc
    11221 gataacctta ttttctctcc ggaatttctc cgtgaaggta aagcccttta cgataatctc
    11281 catccttcac gtattgtcat tggtgagcgt tcagaacgcg cagaacgttt tgcggcgtta
    11341 ttacaggaag gcgcgattaa gcaaaatatc ccgaccctgt ttaccgactc cactgaagca
    11401 gaagcgatta aacttttcgc taatacctat ctggcgatgc gcgtagcata ctttaatgaa
    11461 ctggatagct atgcagaaag tttaggtctg aatactcgcc agattatcga aggtgtttgt
    11521 ctcgacccgc gtatcggcaa ccattacaac aatccgtcgt ttggttatgg tggttattgt
    11581 ttgccgaaag ataccaagca gttactggcg aactaccagt ctgtgccgaa taacctgatc
    11641 tcggcaattg tagatgcaaa ccgcacgcgt aaagatttta ttgccgatgc cattttgtca
    11701 cgcaagccgc aagtggtggg tatttatcgt ctgattatga agagcggttc agataacttc
    11761 cgcgcgtctt ccattcaggg gattatgaag cgtatcaagg cgaaaggcgt tgaagtgatc
    11821 atatacgagc cagtgatgaa agaagactca ttcttcaact ctcgcctgga acgagatctc
    11881 gccaccttca aacaacaagc cgacgtcatt atttccaacc gtatggcaga agagcttaag
    11941 gatgtggcag acaaagtcta cacccgcgat ctctttggca gtgactaaca tcctgttatc
    12001 agggcgattt tcgccctgat tctcttatgt tccctttgta ataattcatt atttttatca
    12061 tttatcctat agcattcatg gcaattatcg ctaaactatg gcgacttaaa attatttcgt
    12121 ccgttagggt aatgatgaga gtagaaaata ataatgtttc tgggcaaaac catgacccgg
    12181 aacagattga tttgattgat ttactggtgc agttgtggcg tggcaagatg acaattatca
    12241 tctctgtcat tgtggctatt gccctggcta tagggtatct ggccgttgcc aaagaaaagt
    12301 ggacttccac agctatcgtt actcaacctg atgtggggca aatcgctggt tataccaacg
    12361 cgatgaatgt gatctacgga tcggctgtac cgaaagtctc cgacattcag gcttcactta
    12421 tcggacgtta cagcactgca ttctcagcat tagcagaaac gctggataac caggaagaag
    12481 cagaaaagct gaccattgag cctaccgtta aaaatcagtc attaccttta gcggtgtctt
    12541 atgtgggtga aacacctgaa ggtgcgcaga aacagctggc gaagtatatc cagcaagttg
    12601 acgaccaggt aaatgaagaa ctggaaaaag acctgaaaga caacatcgcg ctgcgtatga
    12661 aaaacttgca ggattcgctg aagacccagg aagtggtcgc gcaggagcag aaagatctgc
    12721 gtatccgtca gattcaggaa gcgttgcagt atgcgaatca ggcgcaggtg acaaagccac
    12781 aggttcagca gactgaagat gtgacacaag acacgttgtt ccttttgggg agcgaagcgc
    12841 tggagtcgat gattaagcat gaggcgaccc gtccgttggt gttctcgcca aactactatc
    12901 agactcgtca aaacctgctg gatatcgaaa gcttaaaagt tgatgatctt gatattcatg
    12961 cttaccgtta tgtgatgaaa ccgacgttac ctattcgtcg cgatagtccg aaaaaggcaa
    13021 tcaccttgat tctggcggtg ctgctgggcg gcatggttgg cgcggggatt gtgctggggc
    13081 gtaacgctct gcgtaattac aacgcgaagt aatctttttg gttttaaaga aaacgggcag
    13141 ggtggtgaca ccttgcccgt tttttttgcc ggatgcgaca acaatatcgc atccgcttac
    13201 cccgcaactc actgatgccg tttacgcagg ttctcaatta ccgtcgttaa atccagatcc
    13261 tgatcctgca acagcaccag caggtgatat accaagtcag atgcttcgtt ggtcagctca
    13321 aagcggtcat gtacagtcgc ggccagtgcg gtttccacgc cttcttcgcc cactttctgc
    13381 gcaatgcgtt tggtgccgct ggcatacagt ttggcggtat aggaggtttc cgggtcggca
    13441 gatttacgtt cggcgagcag ttgttccagt tgatacagga acagccactg atgagcggtg
    13501 tcgccgaaac agctgctggt gcctttgtgg caggttggcc cgatgggatt cgccagcacc
    13561 agtaacgtgt cgttgtcgca gtccggcgca atactcacca cattgaggaa attgcccgac
    13621 gtttcgcctt tggtccacag tcgctgttta gtgcgcgaga agaaggtgac tttgccgctt
    13681 tcgatggttt tgtctaaggc ttccgggttc atatagccca gcattaacac ttcgccggat
    13741 acagcgtgtt gcacaatcac cggcatcagt ccgtcggttt tttcccagtc cagttcgcga
    13801 cgttgttgtt ctgttaacat atcctgatct ccacgccctg tgttgccagg tacgctttta
    13861 attcaccaat attgattatt tgtttgtgga ataccgaagc tgccagcgca ccgtcaacgt
    13921 cggcatcgcg gaaggcttcg aggaagtgtt ccatggtgcc cgcgccaccg gaggcaatca
    13981 gcgggacgtg gcaaacttca cgtacttttt tcagctgccc gaggtcgtaa ccgttacgca
    14041 cgccgtcctg attcatcatg ttgaggacga tttctccggc accgcgtttt tgcacttcct
    14101 ctacccagtc gagcgtttcc cattgagtga cgcgggtgcg gct
//
