LOCUS       GU068046               13697 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain L119B/10 serogroup O173 O antigen gene
            cluster, complete sequence.
ACCESSION   GU068046
VERSION     GU068046.1  GI:307340827
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13697)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 13697)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..13697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L119B/10"
                     /db_xref="taxon:562"
                     /note="serogroup: O173"
     gene            2015..3214
                     /gene="wzx"
     CDS             2015..3214
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43886.1"
                     /db_xref="GI:307340828"
                     /translation="MNKKVLLNIFSLLSIQGVGYILPLITLPYLVRILGPNNYGVYGY
                     SLAIIQYSILIIDFGFNLTVTKKISLNRDDKLIVSSVFWNVMACKLLLCIFTFLITVA
                     WSRIDNTFAGDNLAVLMSSYTMVIGILIFPIWLFQGKEEITKIALINISSKFLTVPLI
                     FILIDKPSDGWKVGLINGSGYIVAGIIGLHIVSRRNWIVWVKPTFSQMFREYASAWHI
                     FISNASINIYTSSITVILGALTSPVTVGYFVAADKIRLAFQGLINPVSQALYPRINAL
                     MKESPAAGFKTIRLLLRTQGVFFFFCSGFIFYLSPNIISFLYGESYKESIPILKILAW
                     LPFIIAISNVLGYQTLLVLNMERIFSRLVLVGGGIALVIIFPLIIYFGGYGAALTILI
                     AEVSVNL"
     misc_feature    2015..13498
                     /note="O antigen gene cluster"
     gene            3265..4026
                     /gene="wcmU"
     CDS             3265..4026
                     /gene="wcmU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmU"
                     /protein_id="ADN43887.1"
                     /db_xref="GI:307340829"
                     /translation="MIKFSVVTVVFNSAEAFSKTINSVKKQTYSNVEYIVIDGGSTDG
                     TLSLIKENINFISRFVSEEDKGIYDAMNKAINLASGDWIIFINAGDVFYDERILEKTA
                     SFIRLNENKQPDLIGGEVMYDVSGKYEKGEVKSLSKRWVCMPSCHQSLFIRVSIHKKY
                     LYNIDYTICADHELVIRLLNEGCTFLPYRKIISVFSWDGVSALNRISLYKEKLQIALK
                     YNAPLLDIFKLRLLILRVKLSGFLRSIKIKLSKLL"
     gene            4097..5218
                     /gene="gmd"
     CDS             4097..5218
                     /gene="gmd"
                     /note="GDP-mannose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ADN43888.1"
                     /db_xref="GI:307340830"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTGNSKFHLHYGDLTDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAEQVGIKLRF
                     EGTGVDEKGIVVSVTGEDASAVKPGDVIVKVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            5221..6189
                     /gene="fcl"
     CDS             5221..6189
                     /gene="fcl"
                     /note="GDP-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ADN43889.1"
                     /db_xref="GI:307340831"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNPHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDCFRGE"
     gene            6189..6689
                     /gene="gmm"
     CDS             6189..6689
                     /gene="gmm"
                     /note="GDP-mannose mannosyl hydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="ADN43890.1"
                     /db_xref="GI:307340832"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFNHDPSAVIGLDK
                     KEVKNV"
     gene            6682..8130
                     /gene="manC"
     CDS             6682..8130
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADN43891.1"
                     /db_xref="GI:307340833"
                     /translation="MSNVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQST
                     LLRLSSLSCETPLVITNEQHRFVVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFTA
                     LKRNEKQDPVLLILAADHIIRKADIFCDAIQKSISVAEKGHIVTFGIIPEYAETGYGY
                     IEKGDVFKITSNVTNDSFYHVQQFVEKPNRSKAEEYVSSEKYLWNSGMFMFKASVYLE
                     ELKKHRPDIYKICEQAISSSYNDLDFIRLPKNIFKDCPSESIDFAVMEKTKNCVVCPV
                     DIGWSDVGSWHSLWDVSEKTQTGDACKGDIITYKTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKTDVQDVKKIVEMLKEQERSEYISHREEFRPWGKFDAIDQGDRYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNDKTVLLTANESIYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYRIDD"
     gene            8135..9502
                     /gene="manB"
     CDS             8135..9502
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADN43892.1"
                     /db_xref="GI:307340834"
                     /translation="MKNLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKRKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDFQLMEKKTNDI
                     LSILK"
     gene            9551..10567
                     /gene="wcmV"
     CDS             9551..10567
                     /gene="wcmV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmV"
                     /protein_id="ADN43893.1"
                     /db_xref="GI:307340835"
                     /translation="MLNMYNMNIKNSEIDIVVLWVDSEDVKWRTEFELYSSKLIDGDK
                     DNIRFRDYNLIKYWFRGIEKFCPWARKVHFVTSGHYPNWLNLNNKRLNFVKHSDYIPS
                     EFLPTFNSHVIELNLHRIKELSENFVYFNDDFFVIDHISDEHFFSGDIPNDCAIFSPI
                     VPGGLSHILINNVEIINKYYHKSNVIKKDFWKWYSPKYGLNLLRTICLTPWPHFVGFK
                     DTHLPVSFKKSTFVELWEKEPEIMEKTCFSKFRSNQDVSQYLVRYWQLVKGVFNPKNT
                     MKYASYNVLSNSSLYEIKQVIEKQKKQIIIINDGDDIDFNYAKLELEKSFELILPEKS
                     SFEN"
     gene            10570..11649
                     /gene="wzy"
     CDS             10570..11649
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43894.1"
                     /db_xref="GI:307340836"
                     /translation="MLETYFIYNFIMLCGIFFAFVKENTHSIIFKVASSLMLIFVIWI
                     PSGIRYGIGTDYFSYLRIFQSALEGRVTTEIGYYLINYLVVVTGGEGYYIFLISSLII
                     ALVIVINMPRHYSCLYVLIFFCIIYLPSFSLVRQSLAVVFLLVSIKHAIENKILLSYF
                     FALVAISFHLSSLLILPFIILRKVRVPPLLALCMIVFCIIFFLSTNILQDIINSSFIA
                     STKYGFYADTKFGGEAIIGTGGGVLIKALLPLMIILQSSKIVKSQPNCNIIILLSIVA
                     ICASIASLRIHIFSRVSDLFMFLPLLGVTYLMNSFSKKITKQICACLIFLSMIVLFER
                     TIATNLSENNSGLGVSPYTTIFDVR"
     gene            11639..12424
                     /gene="wcmW"
     CDS             11639..12424
                     /gene="wcmW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmW"
                     /protein_id="ADN43895.1"
                     /db_xref="GI:307340837"
                     /translation="MLGKEMYNPLISVIMPAYNAENTIRKSVLSVLNQSYTNFELIIC
                     DDGSTDETKKIILTFSDARIKFINNKYEKGAAGARNSAIDICSGEYVSFLDSDDIWAS
                     DKIEKQLSFMIKNDCDFSYGDYFTFKDDDNTGLFLAPVKTSYEDLLKTCSIGCLTVML
                     HKKLLINQRFPSGYKEDYQLWLRILKDGIIAANYGSADSYYRLGKKHVVHQIKLMNLK
                     KQWYVIRLQHVGYIKTVCYLLTYVYFGLKKHCQAYRSGLGVKI"
     gene            12425..13498
                     /gene="wcmX"
     CDS             12425..13498
                     /gene="wcmX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmX"
                     /protein_id="ADN43896.1"
                     /db_xref="GI:307340838"
                     /translation="MQKVSILIADITCVGGIERVICLLANALANYGFKVEIISLYRTN
                     ANINYALNKDIKVTFINKLKYSGKPGGYFRLFKHILSAVKLKKHLLANKNSFFIINTF
                     PMAFISMPFILFSKNTCVVEHVYHSYYSKPIQYIRQFIYKFITTIVALTDTDAEKYKR
                     GHRHVVKIPNPLSFVECGESTLRNHKIIAVGRLEYQKGFDLLIQAFARASKDTDWSLD
                     IYGDGTLRKELEEIIQFNEISNINLLGNVSNIDEIYKDYSLFVFSSRFEGFGMVLLEA
                     MRAGLPCISFNCPTGPAEIFDNGEYGILVDNGNIDELSNVMKMFMDSFELRSKFSKLS
                     IIRTENYTLDKILPLWVDLINRN"
BASE COUNT     4199 a   2416 c   2985 g   4097 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgggtaatta atacgaaacc attgatagca
       61 atatatctaa aaattttgaa ctggagacgt tgagtataga atgaccgaaa taatagcttt
      121 tttataccaa ataataaaac gctctgctta aaaattaaaa tgtaccttaa ttaaggacga
      181 aatctttaag atttatctat cgccaagttc aattgattag aaaaaacata gaaaccaggg
      241 aaagatcacg taacgctgcc agggaaagat catcagaagt tcactttttg tactaaataa
      301 ttcgcatttt atgtttaaaa attgagatat tccttattac ctaaagctgt ttttcacagc
      361 ttacatatga tcaaatactc cttacataaa taaggagaac aaaatggaac ttgaaaaatt
      421 gatggaacat atttctatta tccccgatta cagacaagac tggaaagtag aacataaatt
      481 gtcagatatt ctactgttga ctatttgcgc cgttatttct ggtgcagaag gttgggaaga
      541 tatagaggat tttggggaaa cacatctcga ttttttgaag caatatggtg attttgaaaa
      601 tggtattcct gttcacgata ccattgccag agttgtatcc tgtatcagtc ctgcaaaatt
      661 tcacgagtgc tttattaact ggatgcgtga ctgccattct tcagatgata aagacgtcat
      721 tgcaattgat ggaaaaacgc tccggcattc ttatgacaag agtcgccgca ggggagcgat
      781 tcatgtcatt agtgcgttct caacaatgca cagtctggtc atcggacaga tcaagacgga
      841 tgagaaatct aatgagatta cagctatccc agaacttctt aacatgctgg atattaaagg
      901 aaaaatcatc acaactgatg cgatgggttg ccagaaagat attgcagaga agatacaaaa
      961 acagggaggt gattatttat tcgcggtaaa aggaaaccag gggcggctaa ataaagcctt
     1021 tgaggaaaaa tttccgctga aaaaattaaa taatccagag catgacagtt acgcaattag
     1081 tgaaaagagt tacggcagag aagaaatccg tcttcatatt gtttgcgatg tccctgatga
     1141 acttattgat ttcacgtttg aatggaaagg gctgaagaaa ttatgcgtgg cagtctcctt
     1201 tcggtccata atagcagaac aaaagaaaga gccagaaatg acggtcagat attatatcag
     1261 ttctgctgat ttaaccgctg agaaattcgc cacagcgatc cgaaatcact ggcacgtgga
     1321 gaataagctg cactggcgtc tggacgtggt aatgaatgaa gacgactgca aaataagaag
     1381 aggaaatgca gcagaattat tttcagggat acggcacatt gctattaata ttttgacgaa
     1441 tgataaggta ttcaaggcag ggttaagacg taagatgcga aaagcagcca tggacagaaa
     1501 ctatctggcg tcagtccttg cggggagcgg gctttcgtaa tcttgccctg gaaatcaggg
     1561 acggttaaaa aattctttga gttttattga tttcacgtga atgaaaaaga ctgtagaaat
     1621 tatgcatggc agtctccttt tggtcaataa tagctgaaca aaagaagaag ccagacatgg
     1681 cgctcagata ttatagcagt tctgctgatt taacagcaga caagtttggc aaagcgatcc
     1741 gaaatcattg gcacgtagag cataagctgt actggtgcct gacgtagtaa taaatgaaga
     1801 cgattgtata ataagaagag gaaacgcagc agaattattt tcagagatca ggcatatcgc
     1861 cattaatttt tgacgaaaga taaagtgctc aaggcagtgt taagacgtaa gatgagaaaa
     1921 gaagctatga atagaaaata cccagtgtca gtccttgcgg agggcgggct tttgtaatct
     1981 tttcctggcc atgcgagctg tatagggctg taaaatgaat aaaaaagtat tattaaatat
     2041 attttcatta ttaagtattc aaggggttgg ttatatcctg ccattaatta ctttgccata
     2101 tcttgttcgg attttgggac ctaataacta tggtgtatat gggtattcct tagctataat
     2161 acagtattcg atattaataa tcgattttgg atttaacctg acggtaacaa aaaaaatatc
     2221 tttaaataga gatgataagc taatagttag ttccgttttt tggaatgtaa tggcatgcaa
     2281 gttattactt tgtattttta catttttgat tactgttgct tggtctagaa tagacaacac
     2341 ttttgcagga gataatctag ctgtactaat gtcatcatac acgatggtaa ttggtatttt
     2401 aatatttcct atatggttgt ttcagggaaa ggaagaaatt acaaaaattg cacttatcaa
     2461 tatatcttcg aaatttttaa cggtgccatt aatttttatt ctaattgata aaccgagtga
     2521 tggttggaaa gtggggctta tcaatggtag tgggtatatt gttgctggaa ttattggttt
     2581 acatatagta tctagaagaa attggattgt atgggtaaaa cccactttta gtcagatgtt
     2641 tcgagagtat gcctctgctt ggcatatttt tatttcaaat gcatccataa atatatatac
     2701 cagtagtatt acagtaatac ttggagcgtt aacgtcacca gttactgtag gatattttgt
     2761 tgcagcagat aaaataagat tagccttcca ggggcttata aatcccgtat cacaagcgtt
     2821 gtacccgagg attaatgcgt taatgaaaga gtcaccggca gcaggtttta aaacaataag
     2881 attattgtta aggactcagg gcgtattttt ctttttttgt agcggtttca ttttttattt
     2941 aagcccaaat ataatttcgt tcttgtatgg agaaagttat aaagaatcca ttcctatatt
     3001 aaaaatatta gcatggctac catttatcat agcaattagt aatgtgttag gatatcaaac
     3061 tttactagtg ttaaatatgg aaagaatatt tagtcgatta gttttagttg gcggtgggat
     3121 tgcgttagtt attatatttc cattaataat ttatttcggt ggttacggcg ctgcattaac
     3181 tattcttatt gctgaagtgt ctgttaactt ataaatgctt acaactctaa tcaaaaaaaa
     3241 ggttcctata tttaaaggcc aataatgatt aaattctcag tagtaacggt tgtatttaat
     3301 tcagcggaag cattttcgaa aaccattaat agtgttaaga aacaaacata ctctaatgtc
     3361 gaatatattg ttatcgacgg tggttctaca gatggtactc tgtcgctaat aaaggaaaat
     3421 attaatttta ttagccgctt tgtcagcgaa gaagataaag ggatttatga tgccatgaat
     3481 aaagcgatta atttggcgtc aggtgattgg attattttta taaatgctgg tgatgttttt
     3541 tatgatgagc gtatcttgga aaaaacggca tcttttatta gattgaatga gaataaacaa
     3601 cctgatctta ttggtgggga agtaatgtat gacgtctctg gtaagtatga aaaaggtgaa
     3661 gtaaagtcat tgtcaaaaag atgggtttgt atgccttcat gtcatcaatc tttatttata
     3721 agagtctcga tacataaaaa gtatctatac aatatagatt atactatctg tgctgatcat
     3781 gagttagtta ttcgccttct taacgaagga tgcacatttt tgccatatag gaaaataatt
     3841 tccgtattct cgtgggatgg tgtgtccgca cttaatagaa tttctttata caaagagaaa
     3901 ttacaaatcg cacttaaata caacgcaccg ttattagata tttttaaatt gcgcctgctt
     3961 attttaagag tgaaattaag tggtttctta aggtccatta aaatcaaact ctcaaagtta
     4021 ttatgatacc acataataat ttggtttttt atttaatgtg ttgtaaatta actcaagaaa
     4081 attgaatgga gaaataatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaggacgg
     4141 ttcttacctt gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg
     4201 tgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatcctc acaccggtaa
     4261 ctctaaattc catttgcatt acggggattt gacagatacc tctaacctga cgcgcatttt
     4321 gcgtgaagtg cagccggatg aagtgtacaa cctgggcgca atgagccacg ttgcggtctc
     4381 ttttgagtca ccagaatata ccgcagatgt tgatgcgatg ggtacgctgc gcctgctgga
     4441 ggcgatccgc ttcctcggtc tggaaaagaa aactcgtttc tatcaggctt ccacttctga
     4501 actgtacggt ctggtgcagg aaattccgca gaaagaaacc acgccgttct acccacgctc
     4561 tccgtatgcg gtcgccaaac tgtatgccta ctggatcacc gttaactacc gcgaatccta
     4621 cggcatgtac gcctgtaacg gaattctctt caaccatgaa tccccgcgcc gcggcgaaac
     4681 cttcgttacc cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg
     4741 cctgtacctc ggcaatatgg attccctgcg tgactggggc catgccaaag actacgtaaa
     4801 aatgcagtgg atgatgctgc aacaggaaca gccggaagat ttcgttattg ctaccggcgt
     4861 tcagtactcc gtacgtcagt tcgtggaaat ggcggcggag caagtcggaa tcaaactgcg
     4921 ttttgaaggc acgggtgtag atgagaaggg gatagtggtt tctgttactg gtgaggatgc
     4981 ctcagcagtt aaacccggag acgtaatagt taaggttgac ccgcgttact tccgtccggc
     5041 agaagttgaa acgctgctcg gcgacccgac caaagcgcac gaaaaactgg gctggaaacc
     5101 agaaatcacc ctcagagaga tggtgtctga aatggtggct aatgacctcg aagcggcgaa
     5161 aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc
     5221 atgagtaaac aacgcatttt tatcgctggt catcgcggga tggtcggttc tgccatcagg
     5281 cggcagctcg aacagcgcgg tgatgtggag ctggtattac gtacccgcga tgaactgaac
     5341 ctgctggaca gccgcgcggt gcatgatttc tttgccagcg aaagcattga ccaggtctat
     5401 ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca cctatccggc ggatttcatc
     5461 tatcagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa cgacgtgaac
     5521 aaactgctgt ttctcggatc gtcctgcatt tacccgaaac tggcaaaaca accgatggca
     5581 gaaagcgagt tgttgcaggg cacgctggag ccgaccaacg agccttacgc cattgccaaa
     5641 atcgccggga tcaaactgtg cgaatcttac aatcgccagt acggacgcga ttaccgttca
     5701 gtcatgccga ccaacctgta cgggccgcac gacaacttcc acccgagtaa cccgcatgtg
     5761 atccctgcat tgctgcgccg cttccacgag gcgacggcac agaatgcacc ggacgtggtg
     5821 gtgtggggca gcggtacacc gatgcgcgaa tttctgcacg tcgatgatat ggcggcggcg
     5881 agcattcatg tgatggagct ggcgcacgaa gtctggctgg agaacaccca accgatgctg
     5941 tcgcacatta acgttggtac gggcgttgac tgcactatcc gcgagctggc gcaaaccatc
     6001 gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg
     6061 ccgcgcaaac tgctggatgt gacgcgcctg catcagcttg gctggtatca cgaaatctca
     6121 ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcgaga ctgctttcgg
     6181 ggggaatgat gtttttacgt caggaagact ttgccactgt agtgcgctcc acaccgcttg
     6241 tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc aaaagaacca
     6301 accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc
     6361 tggaagccgc atttgagcgg ctgacgatgg cggaactggg gctgcgtctg ccgataacag
     6421 caggccagtt ttacggtgtc tggcagcact tttatgacga taacttctcc ggcacggatt
     6481 tcaccactca ctatgtggtg ctcggttttc gcttcagagt cgctgaagaa gagctgttac
     6541 tgccggatga gcagcatgac gattaccgct ggctgacgcc ggacgcactg ctcgccagtg
     6601 acaatgttca cgctaacagc cgcgcctatt ttaatcatga tccaagtgct gttattggtt
     6661 tagataaaaa agaggttaaa aatgtctaat gtaccgttga ttgctgttgt tatggctggt
     6721 ggtacgggta gccgtctttg gccactctca agagagcact atccaaagca atttctgcaa
     6781 ttatcaggcg agaatacttt attacagtca actttattac gattatcctc cctttcttgc
     6841 gaaactccat tagtaataac taacgagcag catcgttttg tggtcgcaga acaattacgt
     6901 caaattgatc aattaagaga taatattatt ctcgagccat gtggaagaaa tactgcaccc
     6961 gcaatagctc tttcagcatt tactgcttta aagcgaaacg aaaagcaaga ccctgttctc
     7021 ctgattctcg ctgctgatca tatcataaga aaagcagata ttttttgtga cgctattcaa
     7081 aaatcgatct ccgtagctga aaaggggcat attgtaacct ttggaattat acctgaatat
     7141 gcggaaacag gttatggtta tatagaaaaa ggagatgttt ttaaaataac atcaaatgta
     7201 actaatgata gtttttatca tgttcagcaa tttgttgaaa aacctaatcg ctcaaaggca
     7261 gaagagtatg tttcttcaga aaaatacctc tggaatagtg gaatgtttat gtttaaagcc
     7321 tcagtttatt tagaggagtt aaaaaaacac agacctgata tatacaaaat ctgtgagcaa
     7381 gcaatttcct catcctataa tgatcttgac ttcatacgat tgcctaaaaa tatctttaaa
     7441 gattgtccgt cagaatcaat tgattttgcg gttatggaaa aaacaaaaaa ttgcgtagtc
     7501 tgtcctgtag atattgggtg gagtgatgta ggctcctggc attccttatg ggatgttagt
     7561 gaaaagacgc agaccggaga tgcctgtaaa ggtgatatta tcacatataa aacaaagaac
     7621 aattatatat actctgaatc agccttagta gctgctgtag gtgtagaaga catagttatt
     7681 gttcaaacta aagacgcgat tctcgtttca aaaaaaactg atgttcagga tgtaaaaaaa
     7741 atagttgaaa tgcttaagga acaggaacgt tcagaatata tatcgcatcg agaggagttt
     7801 cggccttggg ggaaatttga tgctatagat cagggggatc gatataaagt taaaaagata
     7861 atcgtgaaac ccggggaagg gctatcctta cgtatgcacc atcatcgttc tgagcattgg
     7921 attgtacttt ccggtacagc aaaagtaact ttaaatgata aaacagtatt gcttacagca
     7981 aatgaatcaa tctacattcc tctaggggca acatatagcc ttgaaaatcc tggaattatc
     8041 cctctcaatc ttattgaagt cagttcaggt gattatttag gcgaagatga tattgttcgg
     8101 caaaaagaac gttacagaat agatgactaa atacatgaaa aatttaactt gctttaaagc
     8161 ctatgatatt cgcggaaaat taggcgaaga actgaatgaa gatattgcct ggcgcattgg
     8221 tcgcgcttat ggcgaatttc tcaaaccgaa aaccattgtg ttaggcggtg atgtccgcct
     8281 caccagcgaa accttaaaac tggcgctggc gaaaggttta caggatgcgg gcgttgacgt
     8341 gctggatatt ggtatgtccg gcaccgaaga gatctatttc gccacgttcc atctcggcgt
     8401 ggatggcggc atcgaagtta ccgccagcca taacccgatg gattacaacg gcatgaagct
     8461 ggtgcgcgaa ggggctcgcc cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct
     8521 ggctgaagcc aacgactttc ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat
     8581 caacctgcgt gacgcttacg ttgatcacct gttcggttat atcaacgtca aaaacctcac
     8641 gccgctcaag ctggtgatca actccgggaa cggcgcagcg ggtccggtag ttgacgccat
     8701 cgaagcccgc tttaaagccc tcggcgcacc cgtggaattg atcaaagtgc acaacacgcc
     8761 cgacggcaat ttccccaacg gtattcctaa cccgctgctg ccggaatgcc gcgacgacac
     8821 ccgcaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgatg gcgattttga
     8881 ccgctgcttc ctgtttgacg aaaaagggca gtttatcgag ggctactaca ttgtcggcct
     8941 gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccacg atccgcgtct
     9001 ctcctggaac accgttgacg tggtgactgc cgcaggtggc acgccggtaa tgtcgaaaac
     9061 cggacacgcc tttattaaag aacgtatgcg caaggaggac gccatctacg gtggcgaaat
     9121 gagcgctcac cattacttcc gtgatttcgc ttactgcgac agcggcatga tcccgtggct
     9181 gctggtcgcc gaactggtat gcctgaaaag aaaaacgctg ggcgaactgg tgcgcgaccg
     9241 gatggcagcg tttccggcaa gcggtgagat caacagcaaa ctggcgcacc ccgttgaggc
     9301 gattaatcgc gtcgaacagc attttagccg cgaggcgctg gcggtggatc gcaccgatgg
     9361 tatcagcatg acctttgccg actggcgctt taacctgcgc tcctccaaca ccgaaccggt
     9421 ggtgcgcctg aatgtggaat cgcgcggtga ttttcagctc atggaaaaaa aaacaaatga
     9481 tatattatca attttaaaat aatatcggct ggaggaaata taatgattag aaatacagta
     9541 aatttattgg atgttaaata tgtataatat gaatataaaa aatagtgaga tagatatcgt
     9601 tgttttatgg gtggatagtg aagatgtgaa gtggagaact gagtttgaac tttattcatc
     9661 gaaattaatt gatggtgata aggataatat tagattcaga gactataact tgataaaata
     9721 ttggtttagg gggatcgaga aattttgtcc gtgggctaga aaagttcatt ttgtaactag
     9781 cggtcattat cctaattggt tgaatttaaa taataaaaga cttaactttg taaagcattc
     9841 tgattatata ccttcagagt ttttacccac gttcaactcg catgttattg agttgaattt
     9901 acatagaata aaagaattgt ccgaaaattt tgtatatttc aacgatgatt tttttgttat
     9961 agatcatata tcagatgaac attttttttc aggtgatatt cccaatgatt gtgctatttt
    10021 tagtccgata gtaccaggtg gattatcaca tattctaata aacaatgttg agataataaa
    10081 caagtattat cataaatcga atgtaatcaa aaaagatttt tggaaatggt attctcccaa
    10141 atatggactt aatttgctcc gtactatatg tctaactcca tggccacatt ttgttggatt
    10201 taaagataca catttaccag ttagttttaa aaaaagcaca tttgttgaat tatgggagaa
    10261 agaacctgag ataatggaaa aaacatgttt ttcaaaattt aggagcaatc aagatgttag
    10321 tcaatattta gtcaggtatt ggcaattagt gaaaggtgtt tttaatccta aaaatactat
    10381 gaaatatgca tcttataatg tactttcaaa ctctagtttg tatgaaataa aacaagtaat
    10441 tgagaaacaa aaaaaacaaa ttatcattat taatgatggc gatgatattg attttaacta
    10501 tgcaaaatta gaactggaga aatcgtttga gttaattctt cctgaaaaat catcatttga
    10561 gaactaataa tgttagagac ttattttatt tataatttta taatgttatg cggtattttt
    10621 tttgcttttg tcaaagaaaa tacccattca ataatattca aagtcgctag ttcattgatg
    10681 ttgatttttg tcatatggat cccgtcggga atacgatacg gcattggaac agattatttt
    10741 agttatttaa gaatatttca aagtgctctg gagggaagag taactactga gatcggctat
    10801 tatctaatta attatttggt ggttgttaca ggcggggaag ggtattatat ttttttaatc
    10861 tcaagtctga ttattgcgtt agttattgtg attaatatgc caaggcatta tagttgcctt
    10921 tatgttttga tctttttttg cataatttat cttccttcct tttccttagt aagacaatcc
    10981 ttggctgtgg tgtttttgct agtgagcata aagcatgcaa tagaaaataa aatactgttg
    11041 tcttattttt ttgcattagt tgcaatttca tttcaccttt catcgttact catacttcca
    11101 ttcataattc ttagaaaagt tagagttcca cctcttttag cattgtgtat gatcgtattt
    11161 tgtataatct tttttttgag tactaatatt ctccaagata taattaatag ttcttttatt
    11221 gcatctacaa aatatggttt ttatgctgat actaagttcg gtggcgaagc gataattgga
    11281 actggtgggg gagtattaat aaaagcatta cttccattaa tgataatttt acagtctagt
    11341 aaaatagtca agtctcagcc aaattgtaac ataataatcc tgttgagcat tgttgctatt
    11401 tgtgcaagta tagcatcatt aagaatacat atatttagtc gagtcagtga tctttttatg
    11461 tttttgccct tgttaggggt tacgtattta atgaattcat tctcaaaaaa aatcacaaag
    11521 caaatctgtg cttgtttgat ttttttatca atgatagttt tatttgaaag aactattgca
    11581 acaaatcttt ctgaaaataa ttccggactt ggagttagtc catatactac aatatttgat
    11641 gttaggtaaa gaaatgtata atcctcttat ttctgtaata atgcctgcgt ataatgctga
    11701 aaatacgatt aggaagtctg tactttctgt attaaaccaa tcttatacaa attttgaact
    11761 cataatatgt gatgatggtt ctactgacga aacaaagaaa ataattctta ctttttcaga
    11821 tgcacgtatt aaatttataa ataataagta tgaaaaaggt gcagcagggg cgcgaaatag
    11881 tgctattgat atctgtagtg gtgaatatgt tagttttctt gatagtgatg atatatgggc
    11941 atctgataaa atagaaaaac aactttcatt tatgataaag aatgactgtg atttctctta
    12001 tggggactac tttactttta aagatgatga taatacaggt ctttttctgg cacctgtaaa
    12061 aaccagttat gaagatcttt tgaaaacatg tagtataggt tgtttgaccg taatgcttca
    12121 taagaaattg ctcattaatc aaagattccc atccggttat aaagaagatt accagttatg
    12181 gctgagaatt ctcaaagatg gcataattgc tgcaaattat gggtcggcag actcttatta
    12241 cagattagga aaaaaacacg ttgttcatca aataaaattg atgaacttaa aaaaacaatg
    12301 gtacgtaatt aggttacaac atgttggcta tattaaaaca gtctgctact tgcttactta
    12361 tgtttatttt ggtttaaaaa aacattgtca ggcgtataga agtggattag gggtgaaaat
    12421 ataaatgcaa aaagtttcaa ttttgattgc cgatattact tgtgtaggtg gcatagaacg
    12481 cgtgatttgc ttattagcca acgcgttagc taattatggg tttaaggtgg aaattatatc
    12541 cttgtaccgt actaatgcta acatcaatta cgctttaaat aaggatataa aagttacttt
    12601 tataaataaa ctaaaatatt cagggaagcc tgggggatat tttcgattat ttaaacatat
    12661 tctgagtgct gtgaaactca aaaaacatct attggctaat aaaaattcat ttttcataat
    12721 aaatacattt ccaatggctt ttatttcaat gccttttatt cttttttcga aaaatacgtg
    12781 tgtagttgag catgtctatc attcatacta ttcaaagcct atacaataca tacgacagtt
    12841 tatttataag tttatcacta caatagtggc gcttacggat acggatgcgg aaaaatataa
    12901 acgtgggcat cgtcatgtag ttaaaatccc aaatccatta agttttgtag aatgtgggga
    12961 aagtactctg agaaatcata agataattgc agttggtagg ctggaatatc aaaaaggttt
    13021 tgatttacta attcaggctt ttgcacgagc atcaaaagat acagattggt cattagatat
    13081 ttatggcgat ggtacattaa gaaaggaatt agaagagatt attcagttta atgaaatttc
    13141 taatataaat ttattaggga atgtaagtaa tattgatgaa atttataagg attattcatt
    13201 gtttgtattt tcttcaaggt ttgaagggtt tggaatggta ctactggagg ccatgagggc
    13261 tggtttgcca tgcatttctt tcaactgccc cacaggacct gcagagatat ttgataatgg
    13321 agaatatgga attctggtag ataatggcaa tattgacgag ttatctaatg taatgaaaat
    13381 gtttatggat agttttgagt tgcgttcaaa gttttctaaa ctatctataa tccgaacaga
    13441 gaactataca ctagataaga tattgccatt gtgggtggat ttaataaata gaaattaagg
    13501 cgtctgggca atatatattt tggttatttt tgtattatgt tattttatgc gttcgtcttc
    13561 atttaaataa aagcatgtta aactgttttt attataacag gtcatactag tatattatat
    13621 agaccgcaca cgcggcaaac ccctgacagg agtaaacaat gtcaaagcaa cagatcggcg
    13681 tcgtcggtat ggcagtg
//
